Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0012346

Physical Location & Seq

Genomic Coordinates Forward (+)

10081045 .. 10085007

Gene Structure 3963 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G010830.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1788 bp

ATGCCTCAGGATTTGCCGTCGGACCAGGAAACGGACGATGCCGAAGCTGAGTTCGTTGAAATTGATCCCTCTGGACGTTACGGACGGTATAAAGAGGTTTTAGGCAAAGGGGCTTTCAAGAAAGTATATAGAGCGTTTGATGAATTGGAAGGAATTGAAGTAGCTTGGAACCAAGTTAAGGTGGCAGATCTATTGCGCAACTCTGAAGATTTGGAGCGCCTTTATTCGGAAGTTCATTTGCTCAAGACTTTGAAGCACAAGAACATTATTAAGTTTTACAACTCTTGGGTTGATACCAAGCATGAAAATATCAACTTCATTACAGAGATTTTCACTTCGGGAAACTTGAGGCAATACCGGAGGAAACATAAGCACGTTGATTTGAGGGCACTAAAAAAGTGGTCTAGGCAGATCTTAGAAGGCCTTCTGTATCTTCACCGGCATGACCCCCCAGTTATTCACCGAGATTTGAAGTGTGACAACATTTTTGTCAATGGGAACCAGGGTGAGGTGAAAATTGGTGACTTAGGACTGGCTGCTATTCTTCGCCAGGCTCGTTCAGCTCACAGTGTTATTGGTACTCCTGAGTTTATGGCGCCAGAGCTTTATGAAGAGGAATATAATGAGCTTGTTGACATTTATGCCTTTGGCATGTGCTTATTAGAGTTGGTGACCTTTGAGTATCCTTATGTTGAGTGTACTAATGCTGCTCAGATATTCAAGAAAGTGACATCAGGAATAAAGCCAGCATCATTGTCAAAAGTGACAGATCCCGGAGTAAAAGCATTTATAGAAAAATGCATTGTGAAAGTATCTGATCGTTTGCCTGCTGAGGCACTTTTGATGGATCCTTTTCTTCAGTCAGATGGGGATCGTGAAAGTATATCTCATTCTCTTCAACCCAGAGCTCATTATCCAGATGCAGAAGGCACTTCTGATCAGAATGTTGGTGTGGATTCTGAAATGAGTAGAGACTTCACGGTACAAGGCCAGAGGAAAGACGACACAATATTTCTGAAACTACGAATAGCAGATTCTACAGGTCATTTTCGAAATATCCACTTTCCATTTGATATTGGTGAAGACACTGCAATTGCTGTTGCTAGTGAAATGGTTCAAGAGTTGGATCTGACAGATCAAGATGTTTCAACAATTGCTGAACTTATTGACTCTGAGATTCAATCCCACATTCCAGATTGGATTTCTGGAGATAATATTGATGTTGATGTGTCAACTTCGAATAGCCCAGTTTCTGACTTTAAGGAAGATGCTTCTTCCCCGATAAGTGAGGTCAGTAATCTAGCATTAGAAAGATTGCCTTCAGGCCGCAAGTATTGGTCCGACTCTCCCAAGGCAGGTAATGGAAACTCTCCAGTGAAGCATGGTTATTCCAACTTGCAGTCTCCAGAGGGATCAGACAATACAAGAGGCACCGATGACAATGATAAATCTCCTGGTAGTCAAGAAAAATCTCCTCGAAACGAAGACGATCCAAATGGTGCAGAAAACTCTGGTTCACTGAATTGCGATAGTCTTGATGCTTCTATTGATCTACATAATAGAAATGGAGCTCATTTATTTGAACCAGAAGAAGTCAAGATCATTGTTGAGAAACTTGAGAAGCTATTGGTGCAACAGAAAAAGGAGCTAGATGAACTCAAAAGGAAGCATGACTCAGAGATATCAGATGTCTTGCATGAACTACCTCCAGCTTTCTGTCAAAAGGTTTTAGATAATTGTAAGTTAAAGATTTCAGACTCTAGAATGCACCATGAGGCACACTGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

OSR1_C Pkinase

Protein Analysis

595

Amino Acids

67.04

Weight (kDa)

5.02

Isoelectric Point (pI)

45.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	31 - 286	1.6e-49	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	31 - 270	1.6e-35	Protein tyrosine and serine/threonine kinase
OSR1_C	PF12202	339 - 393	6.4e-06	Oxidative-stress-responsive kinase 1 C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012346)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G48260 AT3G48260
fragaria_vesca	FvH4_5g06360 FvH4_5g06360
malus_domestica	MD06G1165300.v1.1 MD14G1170800.v1.1
prunus_persica	Prupe.5G164900_v2.0.a1 Prupe.5G164900_v2.0.a1
pyrus_communis	pycom14g14270
rosa_chinensis	RchiOBHm_Chr7g0192481
rosa_laevigata	RLG00000004362
rosa_multiflora	Rmu_sc0003108.1_g000028
rosa_roxburghii	Rroxscaffold_3G00262990
rosa_rugosa	Rorug07G0001700
rosa_samantha	Rh7AG125800 Rh7BG128100 Rh7CG129700 Rh7DG129600
rosa_wichuraiana	Rw7G010830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	197
Acc36I	ACCTGC	1 cut(s)	1346
AccB1I	GGYRCC	2 cut(s)	595, 1430
AciI	CCGC	1 cut(s)	1327
AclWI	GGATC	8 cut(s)	59, 764, 842, 855, 879, 1134, 1420, 1484
AcuI	CTGAAG	3 cut(s)	225, 842, 1305
AcyI	GRCGYC	1 cut(s)	596
AfaI	GTAC	3 cut(s)	580, 700, 984
AfiI	CCNNNNNNNGG	5 cut(s)	31, 178, 226, 508, 1355
AjnI	CCWGG	4 cut(s)	24, 501, 549, 1453
Alw21I	GWGCWC	2 cut(s)	910, 1573
Alw26I	GTCTC	2 cut(s)	966, 1407
AlwI	GGATC	8 cut(s)	59, 764, 842, 855, 879, 1134, 1420, 1484
AlwNI	CAGNNNCTG	1 cut(s)	1253
AoxI	GGCC	3 cut(s)	421, 988, 1324
ApeKI	GCWGC	2 cut(s)	536, 707
ArsI	GACNNNNNNTTYG	2 cut(s)	470, 502
Asp700I	GAANNNNTTC	2 cut(s)	423, 1113
AspLEI	GCGC	3 cut(s)	198, 219, 598
AspS9I	GGNCC	2 cut(s)	22, 1338
AsuC2I	CCSGG	1 cut(s)	774
AsuHPI	GGTGA	7 cut(s)	428, 452, 518, 523, 533, 682, 1091
AsuII	TTCGAA	2 cut(s)	1051, 1238
AvaII	GGWCC	2 cut(s)	22, 1338
AxyI	CCTNAGG	1 cut(s)	6
BaeGI	GKGCMC	1 cut(s)	391
BamHI	GGATCC	1 cut(s)	847
BanI	GGYRCC	2 cut(s)	595, 1430
BanII	GRGCYC	2 cut(s)	910, 1573
BbsI	GAAGAC	2 cut(s)	1089, 1491
Bbv12I	GWGCWC	2 cut(s)	910, 1573
BbvCI	CCTCAGC	1 cut(s)	831
BbvI	GCAGC	2 cut(s)	523, 694
BccI	CCATC	2 cut(s)	838, 860
BciT130I	CCWGG	4 cut(s)	26, 503, 551, 1455
BciVI	GTATCC	1 cut(s)	693
BclI	TGATCA	1 cut(s)	937
BcnI	CCSGG	1 cut(s)	774
BcoDI	GTCTC	2 cut(s)	966, 1407
BfaI	CTAG	5 cut(s)	405, 1104, 1301, 1649, 1761
BfmI	CTRYAG	1 cut(s)	1038
BfoI	RGCGCY	2 cut(s)	220, 599
BfuAI	ACCTGC	1 cut(s)	1346
BfuI	GTATCC	1 cut(s)	693
BglII	AGATCT	2 cut(s)	187, 411
BisI	GCNGC	3 cut(s)	537, 708, 1327
BlsI	GCNGC	3 cut(s)	538, 709, 1328
Bme1390I	CCNGG	5 cut(s)	26, 503, 551, 774, 1455
Bme18I	GGWCC	2 cut(s)	22, 1338
BmgT120I	GGNCC	2 cut(s)	22, 1338
BmiI	GGNNCC	5 cut(s)	170, 500, 597, 849, 1432
BmrFI	CCNGG	5 cut(s)	26, 503, 551, 774, 1455
BmrI	ACTGGG	2 cut(s)	446, 1241
BmsI	GCATC	5 cut(s)	28, 758, 910, 1258, 1528
BmuI	ACTGGG	2 cut(s)	446, 1241
BpiI	GAAGAC	2 cut(s)	1089, 1491
BpmI	CTGGAG	4 cut(s)	1227, 1356, 1389, 1692
Bpu10I	CCTNAGC	1 cut(s)	831
Bpu14I	TTCGAA	2 cut(s)	1051, 1238
BpuEI	CTTGAG	3 cut(s)	227, 367, 1637
BpuMI	CCSGG	1 cut(s)	774
BsaBI	GATNNNNATC	1 cut(s)	870
BsaHI	GRCGYC	1 cut(s)	596
BsaJI	CCNNGG	2 cut(s)	502, 1350
BsaWI	WCCGGW	1 cut(s)	357
Bsc4I	CCNNNNNNNGG	5 cut(s)	31, 178, 226, 508, 1355
Bse118I	RCCGGY	1 cut(s)	438
Bse1I	ACTGG	4 cut(s)	452, 537, 1247, 1373
Bse21I	CCTNAGG	1 cut(s)	6
Bse8I	GATNNNNATC	1 cut(s)	870
BseBI	CCWGG	4 cut(s)	26, 503, 551, 1455
BseDI	CCNNGG	2 cut(s)	502, 1350
BseJI	GATNNNNATC	1 cut(s)	870
BseLI	CCNNNNNNNGG	5 cut(s)	31, 178, 226, 508, 1355
BseMII	CTCAG	7 cut(s)	20, 39, 576, 725, 822, 1164, 1689
BseNI	ACTGG	4 cut(s)	452, 537, 1247, 1373
BseRI	GAGGAG	1 cut(s)	1464
BseSI	GKGCMC	1 cut(s)	391
BseXI	GCAGC	2 cut(s)	523, 694
BsgI	GTGCAG	1 cut(s)	1521
BshFI	GGCC	3 cut(s)	423, 990, 1326
BshNI	GGYRCC	2 cut(s)	595, 1430
BsiHKAI	GWGCWC	2 cut(s)	910, 1573
BsiSI	CCGG	3 cut(s)	358, 439, 774
BslI	CCNNNNNNNGG	5 cut(s)	31, 178, 226, 508, 1355
BsmAI	GTCTC	2 cut(s)	966, 1407
BsmI	GAATGC	1 cut(s)	1770
BsnI	GGCC	3 cut(s)	423, 990, 1326
Bsp119I	TTCGAA	2 cut(s)	1051, 1238
Bsp1286I	GDGCHC	3 cut(s)	391, 910, 1573
BspACI	CCGC	1 cut(s)	1327
BspANI	GGCC	3 cut(s)	423, 990, 1326
BspCNI	CTCAG	7 cut(s)	19, 40, 577, 724, 823, 1165, 1688
BspLI	GGNNCC	5 cut(s)	170, 500, 597, 849, 1432
BspMI	ACCTGC	1 cut(s)	1346
BspPI	GGATC	8 cut(s)	59, 764, 842, 855, 879, 1134, 1420, 1484
BspT104I	TTCGAA	2 cut(s)	1051, 1238
BspT107I	GGYRCC	2 cut(s)	595, 1430
BsrFI	RCCGGY	1 cut(s)	438
BsrI	ACTGG	4 cut(s)	452, 537, 1247, 1373
BssAI	RCCGGY	1 cut(s)	438
BssECI	CCNNGG	2 cut(s)	502, 1350
BssNI	GRCGYC	1 cut(s)	596
BssT1I	CCWWGG	1 cut(s)	1350
Bst2UI	CCWGG	4 cut(s)	26, 503, 551, 1455
Bst4CI	ACNGT	3 cut(s)	87, 569, 982
Bst6I	CTCTTC	2 cut(s)	606, 900
BstACI	GRCGYC	1 cut(s)	596
BstBI	TTCGAA	2 cut(s)	1051, 1238
BstC8I	GCNNGC	2 cut(s)	747, 828
BstDEI	CTNAG	9 cut(s)	6, 48, 415, 526, 585, 711, 831, 1173, 1675
BstEII	GGTNACC	1 cut(s)	670
BstH2I	RGCGCY	2 cut(s)	220, 599
BstHHI	GCGC	3 cut(s)	198, 219, 598
BstMAI	GTCTC	2 cut(s)	966, 1407
BstMWI	GCNNNNNNNGC	2 cut(s)	560, 1630
BstNI	CCWGG	4 cut(s)	26, 503, 551, 1455
BstNSI	RCATGY	1 cut(s)	655
BstPI	GGTNACC	1 cut(s)	670
BstSCI	CCNGG	5 cut(s)	24, 501, 549, 772, 1453
BstSFI	CTRYAG	1 cut(s)	1038
BstSLI	GKGCMC	1 cut(s)	391
BstV1I	GCAGC	2 cut(s)	523, 694
BstV2I	GAAGAC	2 cut(s)	1089, 1491
BstX2I	RGATCY	5 cut(s)	187, 411, 769, 847, 1126
BstYI	RGATCY	5 cut(s)	187, 411, 769, 847, 1126
Bsu36I	CCTNAGG	1 cut(s)	6
BsuI	GTATCC	1 cut(s)	693
BsuRI	GGCC	3 cut(s)	423, 990, 1326
BtsI	GCAGTG	2 cut(s)	1086, 1780
BtsIMutI	CAGTG	5 cut(s)	574, 1086, 1380, 1517, 1780
BveI	ACCTGC	1 cut(s)	1346
Cac8I	GCNNGC	2 cut(s)	747, 828
CaiI	CAGNNNCTG	1 cut(s)	1253
CfoI	GCGC	3 cut(s)	198, 219, 598
Cfr10I	RCCGGY	1 cut(s)	438
Cfr13I	GGNCC	2 cut(s)	22, 1338
Csp6I	GTAC	3 cut(s)	579, 699, 983
CviAII	CATG	7 cut(s)	302, 443, 652, 1382, 1670, 1697, 1772
CviQI	GTAC	3 cut(s)	579, 699, 983
DdeI	CTNAG	9 cut(s)	6, 48, 415, 526, 585, 711, 831, 1173, 1675
DinI	GGCGCC	1 cut(s)	597
Eam1104I	CTCTTC	2 cut(s)	606, 900
EarI	CTCTTC	2 cut(s)	606, 900
Ecl136II	GAGCTC	2 cut(s)	908, 1571
Eco130I	CCWWGG	1 cut(s)	1350
Eco147I	AGGCCT	1 cut(s)	423
Eco24I	GRGCYC	2 cut(s)	910, 1573
Eco32I	GATATC	1 cut(s)	1683
Eco47I	GGWCC	2 cut(s)	22, 1338
Eco53kI	GAGCTC	2 cut(s)	908, 1571
Eco57I	CTGAAG	3 cut(s)	225, 842, 1305
Eco81I	CCTNAGG	1 cut(s)	6
Eco91I	GGTNACC	1 cut(s)	670
EcoICRI	GAGCTC	2 cut(s)	908, 1571
EcoO65I	GGTNACC	1 cut(s)	670
EcoRII	CCWGG	4 cut(s)	24, 501, 549, 1453
EcoRV	GATATC	1 cut(s)	1683
EcoT14I	CCWWGG	1 cut(s)	1350
EcoT22I	ATGCAT	1 cut(s)	803
EcoT38I	GRGCYC	2 cut(s)	910, 1573
EgeI	GGCGCC	1 cut(s)	597
EheI	GGCGCC	1 cut(s)	597
ErhI	CCWWGG	1 cut(s)	1350
FaeI	CATG	7 cut(s)	305, 446, 655, 1385, 1673, 1700, 1775
FalI	AAGNNNNNCTT	2 cut(s)	1303, 1335
FatI	CATG	7 cut(s)	301, 442, 651, 1381, 1669, 1696, 1771
FbaI	TGATCA	1 cut(s)	937
Fnu4HI	GCNGC	3 cut(s)	537, 708, 1327
FriOI	GRGCYC	2 cut(s)	910, 1573
Fsp4HI	GCNGC	3 cut(s)	537, 708, 1327
FspBI	CTAG	5 cut(s)	405, 1104, 1301, 1649, 1761
FspI	TGCGCA	1 cut(s)	197
GlaI	GCGC	3 cut(s)	197, 218, 597
GluI	GCNGC	3 cut(s)	537, 708, 1327
GsuI	CTGGAG	4 cut(s)	1227, 1356, 1389, 1692
HaeII	RGCGCY	2 cut(s)	220, 599
HaeIII	GGCC	3 cut(s)	423, 990, 1326
HapII	CCGG	3 cut(s)	358, 439, 774
HhaI	GCGC	3 cut(s)	198, 219, 598
Hin1I	GRCGYC	1 cut(s)	596
Hin1II	CATG	7 cut(s)	305, 446, 655, 1385, 1673, 1700, 1775
Hin6I	GCGC	3 cut(s)	196, 217, 596
HinP1I	GCGC	3 cut(s)	196, 217, 596
HincII	GTYRAC	2 cut(s)	634, 1233
HindII	GTYRAC	2 cut(s)	634, 1233
HinfI	GANTC	7 cut(s)	956, 1034, 1169, 1177, 1343, 1673, 1757
HpaII	CCGG	3 cut(s)	358, 439, 774
HphI	GGTGA	7 cut(s)	428, 452, 518, 523, 533, 682, 1091
Hpy166II	GTNNAC	4 cut(s)	634, 699, 1233, 1517
Hpy8I	GTNNAC	4 cut(s)	634, 699, 1233, 1517
Hpy99I	CGWCG	1 cut(s)	22
HpyAV	CCTTC	5 cut(s)	143, 413, 434, 920, 1329
HpyCH4III	ACNGT	3 cut(s)	87, 569, 982
HpyCH4IV	ACGT	2 cut(s)	76, 375
HpyCH4V	TGCA	8 cut(s)	801, 923, 1091, 1399, 1502, 1633, 1696, 1768
HpyF10VI	GCNNNNNNNGC	2 cut(s)	560, 1630
HpyF3I	CTNAG	9 cut(s)	6, 48, 415, 526, 585, 711, 831, 1173, 1675
HpySE526I	ACGT	2 cut(s)	76, 375
Hsp92I	GRCGYC	1 cut(s)	596
Hsp92II	CATG	7 cut(s)	305, 446, 655, 1385, 1673, 1700, 1775
HspAI	GCGC	3 cut(s)	196, 217, 596
KasI	GGCGCC	1 cut(s)	595
Ksp22I	TGATCA	1 cut(s)	937
LmnI	GCTCC	3 cut(s)	214, 1568, 1645
Lsp1109I	GCAGC	2 cut(s)	523, 694
LweI	GCATC	5 cut(s)	28, 758, 910, 1258, 1528
MaeI	CTAG	5 cut(s)	405, 1104, 1301, 1649, 1761
MaeII	ACGT	2 cut(s)	76, 375
MaeIII	GTNAC	6 cut(s)	77, 476, 521, 670, 727, 763
MfeI	CAATTG	2 cut(s)	1092, 1152
MflI	RGATCY	5 cut(s)	187, 411, 769, 847, 1126
MhlI	GDGCHC	3 cut(s)	391, 910, 1573
MluCI	AATT	8 cut(s)	60, 143, 153, 516, 1092, 1152, 1522, 1735
Mly113I	GGCGCC	1 cut(s)	596
MlyI	GAGTC	4 cut(s)	1163, 1337, 1667, 1751
MmeI	TCCRAC	3 cut(s)	1104, 1365, 1416
Mph1103I	ATGCAT	1 cut(s)	803
MroXI	GAANNNNTTC	2 cut(s)	423, 1113
MseI	TTAA	4 cut(s)	177, 270, 1260, 1745
MslI	CAYNNNNRTG	1 cut(s)	441
MspI	CCGG	3 cut(s)	358, 439, 774
MspR9I	CCNGG	5 cut(s)	26, 503, 551, 774, 1455
MunI	CAATTG	2 cut(s)	1092, 1152
Mva1269I	GAATGC	1 cut(s)	1770
MvaI	CCWGG	4 cut(s)	26, 503, 551, 1455
MwoI	GCNNNNNNNGC	2 cut(s)	560, 1630
NarI	GGCGCC	1 cut(s)	596
NciI	CCSGG	1 cut(s)	774
NlaIII	CATG	7 cut(s)	305, 446, 655, 1385, 1673, 1700, 1775
NlaIV	GGNNCC	5 cut(s)	170, 500, 597, 849, 1432
NmuCI	GTSAC	5 cut(s)	476, 521, 670, 727, 763
NsbI	TGCGCA	1 cut(s)	197
NsiI	ATGCAT	1 cut(s)	803
NspI	RCATGY	1 cut(s)	655
NspV	TTCGAA	2 cut(s)	1051, 1238
PceI	AGGCCT	1 cut(s)	423
PcsI	WCGNNNNNNNCGW	1 cut(s)	553
PctI	GAATGC	1 cut(s)	1770
PdmI	GAANNNNTTC	2 cut(s)	423, 1113
PfeI	GAWTC	3 cut(s)	956, 1034, 1177
PfoI	TCCNGGA	1 cut(s)	772
PkrI	GCNGC	3 cut(s)	538, 709, 1328
PleI	GAGTC	4 cut(s)	1163, 1337, 1667, 1751
PluTI	GGCGCC	1 cut(s)	599
PpsI	GAGTC	4 cut(s)	1163, 1337, 1667, 1751
Psp124BI	GAGCTC	2 cut(s)	910, 1573
Psp6I	CCWGG	4 cut(s)	24, 501, 549, 1453
PspEI	GGTNACC	1 cut(s)	670
PspGI	CCWGG	4 cut(s)	24, 501, 549, 1453
PspN4I	GGNNCC	5 cut(s)	170, 500, 597, 849, 1432
PspPI	GGNCC	2 cut(s)	22, 1338
PstNI	CAGNNNCTG	1 cut(s)	1253
PsuI	RGATCY	5 cut(s)	187, 411, 769, 847, 1126
RsaI	GTAC	3 cut(s)	580, 700, 984
RsaNI	GTAC	3 cut(s)	579, 699, 983
RseI	CAYNNNNRTG	1 cut(s)	441
SacI	GAGCTC	2 cut(s)	910, 1573
SaqAI	TTAA	4 cut(s)	177, 270, 1260, 1745
SatI	GCNGC	3 cut(s)	537, 708, 1327
Sau96I	GGNCC	2 cut(s)	22, 1338
SchI	GAGTC	4 cut(s)	1163, 1337, 1667, 1751
ScrFI	CCNGG	5 cut(s)	26, 503, 551, 774, 1455
SduI	GDGCHC	3 cut(s)	391, 910, 1573
SfaNI	GCATC	5 cut(s)	28, 758, 910, 1258, 1528
SfcI	CTRYAG	1 cut(s)	1038
SfoI	GGCGCC	1 cut(s)	597
SfuI	TTCGAA	2 cut(s)	1051, 1238
SinI	GGWCC	2 cut(s)	22, 1338
SmiMI	CAYNNNNRTG	1 cut(s)	441
SmlI	CTYRAG	3 cut(s)	242, 346, 1616
SmoI	CTYRAG	3 cut(s)	242, 346, 1616
Sse9I	AATT	8 cut(s)	60, 143, 153, 516, 1092, 1152, 1522, 1735
SseBI	AGGCCT	1 cut(s)	423
SsiI	CCGC	1 cut(s)	1327
SspDI	GGCGCC	1 cut(s)	595
SspI	AATATT	2 cut(s)	1011, 1216
SspMI	CTAG	5 cut(s)	405, 1104, 1301, 1649, 1761
SstI	GAGCTC	2 cut(s)	910, 1573
StuI	AGGCCT	1 cut(s)	423
StyD4I	CCNGG	5 cut(s)	24, 501, 549, 772, 1453
StyI	CCWWGG	1 cut(s)	1350
TaaI	ACNGT	3 cut(s)	87, 569, 982
TaiI	ACGT	2 cut(s)	79, 378
TaqI	TCGA	3 cut(s)	1051, 1238, 1477
TasI	AATT	8 cut(s)	60, 143, 153, 516, 1092, 1152, 1522, 1735
TatI	WGTACW	1 cut(s)	698
TauI	GCSGC	1 cut(s)	1329
TfiI	GAWTC	3 cut(s)	956, 1034, 1177
Tru1I	TTAA	4 cut(s)	177, 270, 1260, 1745
Tru9I	TTAA	4 cut(s)	177, 270, 1260, 1745
TscAI	CASTG	5 cut(s)	574, 1093, 1380, 1524, 1787
TseFI	GTSAC	5 cut(s)	476, 521, 670, 727, 763
TseI	GCWGC	2 cut(s)	536, 707
Tsp45I	GTSAC	5 cut(s)	476, 521, 670, 727, 763
TspDTI	ATGAA	7 cut(s)	156, 224, 307, 318, 624, 1668, 1713
TspGWI	ACGGA	2 cut(s)	47, 96
TspRI	CASTG	5 cut(s)	574, 1093, 1380, 1524, 1787
VpaK11BI	GGWCC	2 cut(s)	22, 1338
XbaI	TCTAGA	1 cut(s)	1760
XceI	RCATGY	1 cut(s)	655
XmnI	GAANNNNTTC	2 cut(s)	423, 1113
XspI	CTAG	5 cut(s)	405, 1104, 1301, 1649, 1761
Zsp2I	ATGCAT	1 cut(s)	803

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.