Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0012346

Physical Location & Seq

Genomic Coordinates Forward (+)

26496518 .. 26500540

Gene Structure 4023 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1170800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1899 bp

ATGCCACAGGATTTGTCGTCGGAGCGAGACACCGACGATGCTGATACCGAGTTCGTTGAGGTTGATCCCACTGGTCGATACGGTCGGTATAAAGAGGTTCTAGGAAAAGGGGCTTTCAAGAAAGTATATCGAGCATTTGATGAATTGGAAGGTTTAGAAGTAGCTTGGAATCAGGTTAAGGTGGCGGATCTATTGCGCAACTCTGAAGATTTAGTGCGGCTTTATTCAGAAGTTCATTTGCTTAAGACTTTAAAGCACAAGAACATAATTAAGTTTTACAATTCGTGGGTCGATGCCAAGCATGAGAACATCAACATTATTACAGAGATTTTCACTTCGGGAACTTTACGACAGTACCGGAGAAAGCATAAGCATGTTGATTTGAGGGCGTTGAAGAAATGGTCTAGGCAGATCTTAGAAGGCCTTTGGTATCTTCATAATCATGATCCCCCAGTCATCCATCGAGATTTGAAGTGTGACAACATTTTTGTCAATGGGAACCAAGGTGAGGTCAAAATTGGTGACTTAGGACTCGCTGCTATTCTTCGCCAGGCTCGTTCAGCTCACAGTGTTATTGGTACCCCGGAGTTTATGGCGCCAGAGCTTTACGAAGAGGAATACAACGAGCTTGTAGACATTTATGCCTTTGGCATGTGCTTGTTAGAGTTGGTGACCTTTGAGTATCCATATGTTGAATGTTCCAATGCTGCTCAAATATTCAAGAAAGTGACATCAGGAGTAAAGCCAGCATCGTTGGAAAAAGTGACAGATCCTGGAGTTAAAGCATTCATAGAAAAATGCATTGTGAAAGTTTCAGAACGCTTGCCTGCGAAAGAACTTCTGATGGATCCTTTTCTCCAATCAGATGATAATCGTGAAAGTATATCTCGCTCTCTACGACCCACTCATTATTCAGATGCAGAAGGCAGTTCTGATCAGACTGATATTGAAACAAAGGATACGGACCCTTCTCATGAGACAAGCAGAGACTTCAGTGTGCAAGGTCAGAGGAAAGATGACACGATATTTCTGAAATTACGAATAGCAGATTCTACAGGTCATTTTCGAAATATTCACTTTCCATTTGACATTGGGGAAGACACTGCAATTGCTGTTGCTAGTGAAATGGTTCAAGAGTTAGACCTGACAGATCAAGACGTTTCAACACTTGCTGAAATAATTGACTCCGAAATCCATTCCCACATTCCAGATTGGCCACACGGAGAAGATTCTGGAGACCATATTGATGGTGAGGCCTCAACTCCTTATAGCCATGCTTCTGAGTCTAAGGAGGATGCTTCTCCCTTAATACACGAGGCCCTTCTTTCTAATAGTCTTGCGTTAGAAAGATTGCCTTCAGGCCGCAAGTACTGGTGTGACTCACCCAAGGAAGGTGGTGGAAATTCTCCAGCTAAGTATTTCTTTTCTAATTTGAATTCTCCAGCGGCTTCAGTCACTGCAGGAGGTACTGAAGAAAATGAGAACTCTGCTGGTAGCAGCCCTAGGGAGGAAGATGATGCAAATGGTGCTGGCGCCTCAGATTCACCAAATGGTGGTGTTGAGGAAGATGAGAGATCTCCTGGTGGCCCTAGAGAGGAAAATGATCCAAATGGTGCTGGAGCCTCTGACCCACTGAGTGGTGATAGTCATAATGCAGCTACTGATCCGCATCTTATTAATGGAGTTCTTCCATTTGAATCAGAAGTAAAGATCATTGCCGAGAAACTCGAGAATCTATTGGTAGAACAGAAAAAGGAGTTAGATGAGCTCAAGAAGAAACATGAATTAGCAATATCAGAACCATTGAAAGAACTGCCCCCAGTTTTCCGTCAAAAGGTTTTAGATATATGTAAGATGAGATTTCCAGACTACAAAATGCAGCTTGAGGCAGACTGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0035556 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

OSR1_C Pkinase

Protein Analysis

633

Amino Acids

70.65

Weight (kDa)

4.87

Isoelectric Point (pI)

48.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	31 - 286	1.7e-49	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	31 - 274	1.8e-35	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012346)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G48260 AT3G48260
fragaria_vesca	FvH4_5g06360 FvH4_5g06360
malus_domestica	MD06G1165300.v1.1 MD14G1170800.v1.1
prunus_persica	Prupe.5G164900_v2.0.a1 Prupe.5G164900_v2.0.a1
pyrus_communis	pycom14g14270
rosa_chinensis	RchiOBHm_Chr7g0192481
rosa_laevigata	RLG00000004362
rosa_multiflora	Rmu_sc0003108.1_g000028
rosa_roxburghii	Rroxscaffold_3G00262990
rosa_rugosa	Rorug07G0001700
rosa_samantha	Rh7AG125800 Rh7BG128100 Rh7CG129700 Rh7DG129600
rosa_wichuraiana	Rw7G010830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	197
Acc65I	GGTACC	1 cut(s)	578
AccB1I	GGYRCC	3 cut(s)	578, 595, 1532
AccB7I	CCANNNNNTGG	2 cut(s)	1553, 1637
AccI	GTMKAC	1 cut(s)	633
AciI	CCGC	5 cut(s)	185, 217, 1363, 1445, 1667
AclWI	GGATC	8 cut(s)	59, 195, 440, 764, 842, 855, 1598, 1658
AcoI	YGGCCR	1 cut(s)	1214
AcsI	RAATTY	2 cut(s)	1402, 1435
AcuI	CTGAAG	5 cut(s)	225, 976, 1341, 1434, 1491
AcyI	GRCGYC	2 cut(s)	596, 1533
AdeI	CACNNNGTG	1 cut(s)	1637
AfaI	GTAC	4 cut(s)	356, 580, 1370, 1468
AfiI	CCNNNNNNNGG	7 cut(s)	508, 1391, 1507, 1553, 1594, 1637, 1834
AflII	CTTAAG	1 cut(s)	242
AjnI	CCWGG	3 cut(s)	549, 772, 1579
AluBI	AGCT	8 cut(s)	164, 563, 604, 628, 1412, 1658, 1768, 1882
AluI	AGCT	8 cut(s)	164, 563, 604, 628, 1412, 1658, 1768, 1882
Alw21I	GWGCWC	1 cut(s)	1770
Alw26I	GTCTC	4 cut(s)	21, 971, 981, 1230
AlwI	GGATC	8 cut(s)	59, 195, 440, 764, 842, 855, 1598, 1658
AlwNI	CAGNNNCTG	3 cut(s)	773, 1457, 1661
Ama87I	CYCGRG	1 cut(s)	1727
AoxI	GGCC	6 cut(s)	421, 1214, 1254, 1317, 1360, 1585
ApeKI	GCWGC	5 cut(s)	536, 707, 1497, 1655, 1879
ApoI	RAATTY	2 cut(s)	1402, 1435
ArsI	GACNNNNNNTTYG	2 cut(s)	470, 502
AseI	ATTAAT	1 cut(s)	1677
Asp700I	GAANNNNTTC	1 cut(s)	1128
Asp718I	GGTACC	1 cut(s)	578
AspA2I	CCTAGG	1 cut(s)	1502
AspLEI	GCGC	3 cut(s)	198, 598, 1535
AspS9I	GGNCC	3 cut(s)	964, 1318, 1586
AsuC2I	CCSGG	1 cut(s)	584
AsuHPI	GGTGA	7 cut(s)	518, 533, 682, 1262, 1374, 1536, 1652
AsuII	TTCGAA	1 cut(s)	1066
AvaI	CYCGRG	1 cut(s)	1727
AvaII	GGWCC	1 cut(s)	964
AvrII	CCTAGG	1 cut(s)	1502
BaeI	ACNNNNGTAYC	2 cut(s)	338, 371
BalI	TGGCCA	1 cut(s)	1216
BamHI	GGATCC	1 cut(s)	847
BanI	GGYRCC	3 cut(s)	578, 595, 1532
BanII	GRGCYC	1 cut(s)	1770
BauI	CACGAG	1 cut(s)	1313
BbsI	GAAGAC	1 cut(s)	1104
Bbv12I	GWGCWC	1 cut(s)	1770
BbvI	GCAGC	5 cut(s)	523, 694, 1509, 1667, 1891
BccI	CCATC	3 cut(s)	468, 838, 1241
BciT130I	CCWGG	3 cut(s)	551, 774, 1581
BciVI	GTATCC	2 cut(s)	693, 952
BclI	TGATCA	1 cut(s)	934
BcnI	CCSGG	1 cut(s)	584
BcoDI	GTCTC	4 cut(s)	21, 971, 981, 1230
BfaI	CTAG	5 cut(s)	101, 405, 1119, 1503, 1590
BfmI	CTRYAG	2 cut(s)	1053, 1458
BfoI	RGCGCY	2 cut(s)	599, 1536
BfrI	CTTAAG	1 cut(s)	242
BfuI	GTATCC	2 cut(s)	693, 952
BglII	AGATCT	2 cut(s)	411, 1574
BisI	GCNGC	8 cut(s)	218, 537, 708, 1363, 1446, 1498, 1656, 1880
BlnI	CCTAGG	1 cut(s)	1502
BlsI	GCNGC	8 cut(s)	219, 538, 709, 1364, 1447, 1499, 1657, 1881
BmcAI	AGTACT	1 cut(s)	1370
Bme1390I	CCNGG	4 cut(s)	551, 584, 774, 1581
Bme18I	GGWCC	1 cut(s)	964
BmeT110I	CYCGRG	1 cut(s)	1727
BmgT120I	GGNCC	3 cut(s)	964, 1318, 1586
BmiI	GGNNCC	7 cut(s)	500, 580, 597, 849, 966, 1534, 1621
BmrFI	CCNGG	4 cut(s)	551, 584, 774, 1581
BmrI	ACTGGG	2 cut(s)	446, 1814
BmsI	GCATC	7 cut(s)	28, 283, 758, 907, 1285, 1507, 1678
BmuI	ACTGGG	2 cut(s)	446, 1814
BpiI	GAAGAC	1 cut(s)	1104
BpmI	CTGGAG	5 cut(s)	795, 1254, 1392, 1425, 1638
Bpu14I	TTCGAA	1 cut(s)	1066
BpuEI	CTTGAG	1 cut(s)	1754
BpuMI	CCSGG	1 cut(s)	584
BsaBI	GATNNNNATC	2 cut(s)	870, 1668
BsaHI	GRCGYC	2 cut(s)	596, 1533
BsaI	GGTCTC	1 cut(s)	1230
BsaJI	CCNNGG	4 cut(s)	502, 582, 1386, 1502
BsaWI	WCCGGW	1 cut(s)	357
Bsc4I	CCNNNNNNNGG	7 cut(s)	508, 1391, 1507, 1553, 1594, 1637, 1834
Bse1I	ACTGG	4 cut(s)	76, 452, 1376, 1820
Bse3DI	GCAATG	1 cut(s)	1713
Bse8I	GATNNNNATC	2 cut(s)	870, 1668
BseBI	CCWGG	3 cut(s)	551, 774, 1581
BseDI	CCNNGG	4 cut(s)	502, 582, 1386, 1502
BseGI	GGATG	2 cut(s)	456, 1300
BseJI	GATNNNNATC	2 cut(s)	870, 1668
BseLI	CCNNNNNNNGG	7 cut(s)	508, 1391, 1507, 1553, 1594, 1637, 1834
BseMI	GCAATG	1 cut(s)	1713
BseMII	CTCAG	3 cut(s)	1272, 1551, 1625
BseNI	ACTGG	4 cut(s)	76, 452, 1376, 1820
BseXI	GCAGC	5 cut(s)	523, 694, 1509, 1667, 1891
Bsh1285I	CGRYCG	1 cut(s)	85
BshFI	GGCC	6 cut(s)	423, 1216, 1256, 1319, 1362, 1587
BshNI	GGYRCC	3 cut(s)	578, 595, 1532
BsiEI	CGRYCG	1 cut(s)	85
BsiHKAI	GWGCWC	1 cut(s)	1770
BsiHKCI	CYCGRG	1 cut(s)	1727
BsiSI	CCGG	2 cut(s)	358, 584
BslI	CCNNNNNNNGG	7 cut(s)	508, 1391, 1507, 1553, 1594, 1637, 1834
BsmAI	GTCTC	4 cut(s)	21, 971, 981, 1230
BsmI	GAATGC	1 cut(s)	785
BsnI	GGCC	6 cut(s)	423, 1216, 1256, 1319, 1362, 1587
Bso31I	GGTCTC	1 cut(s)	1230
BsoBI	CYCGRG	1 cut(s)	1727
Bsp119I	TTCGAA	1 cut(s)	1066
Bsp1286I	GDGCHC	1 cut(s)	1770
BspACI	CCGC	5 cut(s)	185, 217, 1363, 1445, 1667
BspANI	GGCC	6 cut(s)	423, 1216, 1256, 1319, 1362, 1587
BspCNI	CTCAG	3 cut(s)	1273, 1550, 1626
BspHI	TCATGA	2 cut(s)	442, 973
BspLI	GGNNCC	7 cut(s)	500, 580, 597, 849, 966, 1534, 1621
BspMAI	CTGCAG	1 cut(s)	1462
BspPI	GGATC	8 cut(s)	59, 195, 440, 764, 842, 855, 1598, 1658
BspT104I	TTCGAA	1 cut(s)	1066
BspT107I	GGYRCC	3 cut(s)	578, 595, 1532
BspTI	CTTAAG	1 cut(s)	242
BspTNI	GGTCTC	1 cut(s)	1230
BsrDI	GCAATG	1 cut(s)	1713
BsrI	ACTGG	4 cut(s)	76, 452, 1376, 1820
BssECI	CCNNGG	4 cut(s)	502, 582, 1386, 1502
BssNI	GRCGYC	2 cut(s)	596, 1533
BssSI	CACGAG	1 cut(s)	1313
BssT1I	CCWWGG	3 cut(s)	502, 1386, 1502
Bst2BI	CACGAG	1 cut(s)	1313
Bst2UI	CCWGG	3 cut(s)	551, 774, 1581
Bst4CI	ACNGT	3 cut(s)	83, 354, 569
Bst6I	CTCTTC	1 cut(s)	606
BstACI	GRCGYC	2 cut(s)	596, 1533
BstAFI	CTTAAG	1 cut(s)	242
BstAPI	GCANNNNNTGC	1 cut(s)	1526
BstBI	TTCGAA	1 cut(s)	1066
BstC8I	GCNNGC	4 cut(s)	747, 824, 828, 1531
BstDEI	CTNAG	7 cut(s)	415, 526, 1281, 1287, 1413, 1537, 1634
BstEII	GGTNACC	1 cut(s)	670
BstF5I	GGATG	2 cut(s)	456, 1300
BstH2I	RGCGCY	2 cut(s)	599, 1536
BstHHI	GCGC	3 cut(s)	198, 598, 1535
BstMAI	GTCTC	4 cut(s)	21, 971, 981, 1230
BstMCI	CGRYCG	1 cut(s)	85
BstMWI	GCNNNNNNNGC	2 cut(s)	560, 1526
BstNI	CCWGG	3 cut(s)	551, 774, 1581
BstNSI	RCATGY	2 cut(s)	377, 655
BstPI	GGTNACC	1 cut(s)	670
BstSCI	CCNGG	4 cut(s)	549, 582, 772, 1579
BstSFI	CTRYAG	2 cut(s)	1053, 1458
BstV1I	GCAGC	5 cut(s)	523, 694, 1509, 1667, 1891
BstV2I	GAAGAC	1 cut(s)	1104
BstX2I	RGATCY	5 cut(s)	187, 411, 769, 847, 1574
BstXI	CCANNNNNNTGG	1 cut(s)	1247
BstYI	RGATCY	5 cut(s)	187, 411, 769, 847, 1574
BsuI	GTATCC	2 cut(s)	693, 952
BsuRI	GGCC	6 cut(s)	423, 1216, 1256, 1319, 1362, 1587
BtsCI	GGATG	2 cut(s)	456, 1300
BtsI	GCAGTG	2 cut(s)	1101, 1455
BtsIMutI	CAGTG	6 cut(s)	69, 574, 1000, 1101, 1455, 1631
Cac8I	GCNNGC	4 cut(s)	747, 824, 828, 1531
CaiI	CAGNNNCTG	3 cut(s)	773, 1457, 1661
CciI	TCATGA	2 cut(s)	442, 973
CfoI	GCGC	3 cut(s)	198, 598, 1535
Cfr13I	GGNCC	3 cut(s)	964, 1318, 1586
Csp6I	GTAC	4 cut(s)	355, 579, 1369, 1467
CspCI	CAANNNNNGTGG	3 cut(s)	29, 1375, 1410
CviAII	CATG	7 cut(s)	302, 374, 443, 652, 974, 1274, 1781
CviQI	GTAC	4 cut(s)	355, 579, 1369, 1467
DdeI	CTNAG	7 cut(s)	415, 526, 1281, 1287, 1413, 1537, 1634
DinI	GGCGCC	2 cut(s)	597, 1534
DraI	TTTAAA	1 cut(s)	252
DraIII	CACNNNGTG	1 cut(s)	1637
EaeI	YGGCCR	1 cut(s)	1214
Eam1104I	CTCTTC	1 cut(s)	606
EarI	CTCTTC	1 cut(s)	606
EciI	GGCGGA	1 cut(s)	200
Ecl136II	GAGCTC	1 cut(s)	1768
Eco130I	CCWWGG	3 cut(s)	502, 1386, 1502
Eco147I	AGGCCT	2 cut(s)	423, 1256
Eco24I	GRGCYC	1 cut(s)	1770
Eco31I	GGTCTC	1 cut(s)	1230
Eco47I	GGWCC	1 cut(s)	964
Eco53kI	GAGCTC	1 cut(s)	1768
Eco57I	CTGAAG	5 cut(s)	225, 976, 1341, 1434, 1491
Eco88I	CYCGRG	1 cut(s)	1727
Eco91I	GGTNACC	1 cut(s)	670
EcoICRI	GAGCTC	1 cut(s)	1768
EcoO109I	RGGNCCY	1 cut(s)	1318
EcoO65I	GGTNACC	1 cut(s)	670
EcoRI	GAATTC	1 cut(s)	1435
EcoRII	CCWGG	3 cut(s)	549, 772, 1579
EcoT14I	CCWWGG	3 cut(s)	502, 1386, 1502
EcoT22I	ATGCAT	1 cut(s)	803
EcoT38I	GRGCYC	1 cut(s)	1770
EgeI	GGCGCC	2 cut(s)	597, 1534
EheI	GGCGCC	2 cut(s)	597, 1534
ErhI	CCWWGG	3 cut(s)	502, 1386, 1502
FaeI	CATG	7 cut(s)	305, 377, 446, 655, 977, 1277, 1784
FalI	AAGNNNNNCTT	4 cut(s)	1339, 1371, 1406, 1438
FatI	CATG	7 cut(s)	301, 373, 442, 651, 973, 1273, 1780
FauNDI	CATATG	1 cut(s)	688
FbaI	TGATCA	1 cut(s)	934
FblI	GTMKAC	1 cut(s)	633
Fnu4HI	GCNGC	8 cut(s)	218, 537, 708, 1363, 1446, 1498, 1656, 1880
FokI	GGATG	2 cut(s)	443, 1307
FriOI	GRGCYC	1 cut(s)	1770
Fsp4HI	GCNGC	8 cut(s)	218, 537, 708, 1363, 1446, 1498, 1656, 1880
FspBI	CTAG	5 cut(s)	101, 405, 1119, 1503, 1590
FspI	TGCGCA	1 cut(s)	197
GlaI	GCGC	3 cut(s)	197, 597, 1534
GluI	GCNGC	8 cut(s)	218, 537, 708, 1363, 1446, 1498, 1656, 1880
GsuI	CTGGAG	5 cut(s)	795, 1254, 1392, 1425, 1638
HaeII	RGCGCY	2 cut(s)	599, 1536
HaeIII	GGCC	6 cut(s)	423, 1216, 1256, 1319, 1362, 1587
HapII	CCGG	2 cut(s)	358, 584
HhaI	GCGC	3 cut(s)	198, 598, 1535
Hin1I	GRCGYC	2 cut(s)	596, 1533
Hin1II	CATG	7 cut(s)	305, 377, 446, 655, 977, 1277, 1784
Hin6I	GCGC	3 cut(s)	196, 596, 1533
HinP1I	GCGC	3 cut(s)	196, 596, 1533
HpaII	CCGG	2 cut(s)	358, 584
HphI	GGTGA	7 cut(s)	518, 533, 682, 1262, 1374, 1536, 1652
Hpy166II	GTNNAC	1 cut(s)	634
Hpy8I	GTNNAC	1 cut(s)	634
Hpy99I	CGWCG	2 cut(s)	22, 38
HpyAV	CCTTC	7 cut(s)	143, 413, 917, 978, 1331, 1365, 1385
HpyCH4III	ACNGT	3 cut(s)	83, 354, 569
HpyCH4IV	ACGT	1 cut(s)	1158
HpyCH4V	TGCA	8 cut(s)	801, 920, 1000, 1106, 1460, 1520, 1655, 1879
HpyF10VI	GCNNNNNNNGC	2 cut(s)	560, 1526
HpyF3I	CTNAG	7 cut(s)	415, 526, 1281, 1287, 1413, 1537, 1634
HpySE526I	ACGT	1 cut(s)	1158
Hsp92I	GRCGYC	2 cut(s)	596, 1533
Hsp92II	CATG	7 cut(s)	305, 377, 446, 655, 977, 1277, 1784
HspAI	GCGC	3 cut(s)	196, 596, 1533
KasI	GGCGCC	2 cut(s)	595, 1532
KpnI	GGTACC	1 cut(s)	582
Ksp22I	TGATCA	1 cut(s)	934
LmnI	GCTCC	2 cut(s)	22, 1619
Lsp1109I	GCAGC	5 cut(s)	523, 694, 1509, 1667, 1891
LweI	GCATC	7 cut(s)	28, 283, 758, 907, 1285, 1507, 1678
MaeI	CTAG	5 cut(s)	101, 405, 1119, 1503, 1590
MaeII	ACGT	1 cut(s)	1158
MaeIII	GTNAC	7 cut(s)	476, 521, 670, 727, 763, 1376, 1453
MfeI	CAATTG	1 cut(s)	1107
MflI	RGATCY	5 cut(s)	187, 411, 769, 847, 1574
MhlI	GDGCHC	1 cut(s)	1770
MlsI	TGGCCA	1 cut(s)	1216
MluNI	TGGCCA	1 cut(s)	1216
Mly113I	GGCGCC	2 cut(s)	596, 1533
MlyI	GAGTC	4 cut(s)	525, 1178, 1292, 1373
MmeI	TCCRAC	1 cut(s)	735
Mox20I	TGGCCA	1 cut(s)	1216
Mph1103I	ATGCAT	1 cut(s)	803
MroXI	GAANNNNTTC	1 cut(s)	1128
MscI	TGGCCA	1 cut(s)	1216
MseI	TTAA	7 cut(s)	177, 243, 251, 270, 780, 1307, 1677
MslI	CAYNNNNRTG	3 cut(s)	372, 441, 1245
Msp20I	TGGCCA	1 cut(s)	1216
MspA1I	CMGCKG	1 cut(s)	1445
MspCI	CTTAAG	1 cut(s)	242
MspI	CCGG	2 cut(s)	358, 584
MspR9I	CCNGG	4 cut(s)	551, 584, 774, 1581
MunI	CAATTG	1 cut(s)	1107
Mva1269I	GAATGC	1 cut(s)	785
MvaI	CCWGG	3 cut(s)	551, 774, 1581
MwoI	GCNNNNNNNGC	2 cut(s)	560, 1526
NarI	GGCGCC	2 cut(s)	596, 1533
NciI	CCSGG	1 cut(s)	584
NdeI	CATATG	1 cut(s)	688
NlaIII	CATG	7 cut(s)	305, 377, 446, 655, 977, 1277, 1784
NlaIV	GGNNCC	7 cut(s)	500, 580, 597, 849, 966, 1534, 1621
NmeAIII	GCCGAG	1 cut(s)	1744
NmuCI	GTSAC	7 cut(s)	476, 521, 670, 727, 763, 1376, 1453
NsbI	TGCGCA	1 cut(s)	197
NsiI	ATGCAT	1 cut(s)	803
NspI	RCATGY	2 cut(s)	377, 655
NspV	TTCGAA	1 cut(s)	1066
PaeR7I	CTCGAG	1 cut(s)	1727
PagI	TCATGA	2 cut(s)	442, 973
PceI	AGGCCT	2 cut(s)	423, 1256
PcsI	WCGNNNNNNNCGW	2 cut(s)	553, 895
PctI	GAATGC	1 cut(s)	785
PdmI	GAANNNNTTC	1 cut(s)	1128
PfeI	GAWTC	6 cut(s)	169, 1049, 1229, 1541, 1697, 1732
PflMI	CCANNNNNTGG	2 cut(s)	1553, 1637
PfoI	TCCNGGA	1 cut(s)	772
PkrI	GCNGC	8 cut(s)	219, 538, 709, 1364, 1447, 1499, 1657, 1881
PleI	GAGTC	4 cut(s)	525, 1178, 1291, 1373
PluTI	GGCGCC	2 cut(s)	599, 1536
PpsI	GAGTC	4 cut(s)	525, 1178, 1291, 1373
PshBI	ATTAAT	1 cut(s)	1677
Psp124BI	GAGCTC	1 cut(s)	1770
Psp6I	CCWGG	3 cut(s)	549, 772, 1579
PspEI	GGTNACC	1 cut(s)	670
PspGI	CCWGG	3 cut(s)	549, 772, 1579
PspN4I	GGNNCC	7 cut(s)	500, 580, 597, 849, 966, 1534, 1621
PspPI	GGNCC	3 cut(s)	964, 1318, 1586
PstI	CTGCAG	1 cut(s)	1462
PstNI	CAGNNNCTG	3 cut(s)	773, 1457, 1661
PsuI	RGATCY	5 cut(s)	187, 411, 769, 847, 1574
RsaI	GTAC	4 cut(s)	356, 580, 1370, 1468
RsaNI	GTAC	4 cut(s)	355, 579, 1369, 1467
RseI	CAYNNNNRTG	3 cut(s)	372, 441, 1245
SacI	GAGCTC	1 cut(s)	1770
SaqAI	TTAA	7 cut(s)	177, 243, 251, 270, 780, 1307, 1677
SatI	GCNGC	8 cut(s)	218, 537, 708, 1363, 1446, 1498, 1656, 1880
Sau96I	GGNCC	3 cut(s)	964, 1318, 1586
ScaI	AGTACT	1 cut(s)	1370
SchI	GAGTC	4 cut(s)	525, 1178, 1292, 1373
ScrFI	CCNGG	4 cut(s)	551, 584, 774, 1581
SduI	GDGCHC	1 cut(s)	1770
SfaNI	GCATC	7 cut(s)	28, 283, 758, 907, 1285, 1507, 1678
SfcI	CTRYAG	2 cut(s)	1053, 1458
SfoI	GGCGCC	2 cut(s)	597, 1534
Sfr274I	CTCGAG	1 cut(s)	1727
SfuI	TTCGAA	1 cut(s)	1066
SinI	GGWCC	1 cut(s)	964
SlaI	CTCGAG	1 cut(s)	1727
SmiMI	CAYNNNNRTG	3 cut(s)	372, 441, 1245
SmlI	CTYRAG	4 cut(s)	242, 1727, 1769, 1883
SmoI	CTYRAG	4 cut(s)	242, 1727, 1769, 1883
SseBI	AGGCCT	2 cut(s)	423, 1256
SsiI	CCGC	5 cut(s)	185, 217, 1363, 1445, 1667
SspDI	GGCGCC	2 cut(s)	595, 1532
SspI	AATATT	2 cut(s)	717, 1072
SspMI	CTAG	5 cut(s)	101, 405, 1119, 1503, 1590
SstI	GAGCTC	1 cut(s)	1770
StuI	AGGCCT	2 cut(s)	423, 1256
StyD4I	CCNGG	4 cut(s)	549, 582, 772, 1579
StyI	CCWWGG	3 cut(s)	502, 1386, 1502
TaaI	ACNGT	3 cut(s)	83, 354, 569
TaiI	ACGT	1 cut(s)	1161
TaqI	TCGA	6 cut(s)	76, 130, 291, 463, 1066, 1728
TatI	WGTACW	1 cut(s)	1368
TauI	GCSGC	3 cut(s)	220, 1365, 1448
TfiI	GAWTC	6 cut(s)	169, 1049, 1229, 1541, 1697, 1732
Tru1I	TTAA	7 cut(s)	177, 243, 251, 270, 780, 1307, 1677
Tru9I	TTAA	7 cut(s)	177, 243, 251, 270, 780, 1307, 1677
TscAI	CASTG	6 cut(s)	76, 574, 1000, 1108, 1462, 1638
TseFI	GTSAC	7 cut(s)	476, 521, 670, 727, 763, 1376, 1453
TseI	GCWGC	5 cut(s)	536, 707, 1497, 1655, 1879
Tsp45I	GTSAC	7 cut(s)	476, 521, 670, 727, 763, 1376, 1453
TspDTI	ATGAA	5 cut(s)	156, 224, 425, 778, 1797
TspGWI	ACGGA	3 cut(s)	977, 1236, 1817
TspRI	CASTG	6 cut(s)	76, 574, 1000, 1108, 1462, 1638
Van91I	CCANNNNNTGG	2 cut(s)	1553, 1637
Vha464I	CTTAAG	1 cut(s)	242
VpaK11BI	GGWCC	1 cut(s)	964
VspI	ATTAAT	1 cut(s)	1677
XapI	RAATTY	2 cut(s)	1402, 1435
XceI	RCATGY	2 cut(s)	377, 655
XhoI	CTCGAG	1 cut(s)	1727
XmaJI	CCTAGG	1 cut(s)	1502
XmiI	GTMKAC	1 cut(s)	633
XmnI	GAANNNNTTC	1 cut(s)	1128
XspI	CTAG	5 cut(s)	101, 405, 1119, 1503, 1590
ZrmI	AGTACT	1 cut(s)	1370
Zsp2I	ATGCAT	1 cut(s)	803

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.