Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0010436

Physical Location & Seq

Genomic Coordinates Forward (+)

30624413 .. 30626515

Gene Structure 2103 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1166300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2103 bp

ATGACTAATCTTTCATTAAACGAACATAACACTAGTCACTCCGCCCACAAGCTTTTTCCCGCTCCCCGGCGCCAACCTGAATCTCTACGGATGAGACCTTTCTCAGCCGTTCTCCTCCTTCTCCTCCTCCACCACCGCTTCGTCTCCCCCACCTCCGCTCTCGACTTCCTCTTCAACTCCTTCTCCAACATCACCAATGCCACCAACCTCACTCTCATCAACGACGCCCGACTCGACGGCGCCGCCATCCGCCTCACAAACGATACCAACCAGTTCGCCGTCGGCCGCGTCTTCTACCCAAAGAAAATTCCCATGAAACCCACCTCCAACTCCACCTCCGTCGCCTCCTTCTCCACCTCCTTCGTCTTCTCCGTCCTTCCGGACATTGCCTCGAGCCCTGGATTCGGCCTCTGCTTCGTTCTCTCCAACTCCACCTCGCCGCCCAATTCCCTCGCCAGCCAATACTTCGGCCTCTTCACCAACACCACCGTCCCCTTCGTCGCCCCGTTGCTAGCCGTCGAGTTCGACACGGGTCAAAACCCGGAATTCAACGACCCAGATGGGAATCACATCGGAATCGACCTTAACAACGTCGATTCCGCCTCCACGACCCCCGCCGGGTACTACAACTCGACCGGAGGATTCGTCCCGGTCCAAATGCGGACCGGGCAGAACGTCCGGGCCTGGATTGAATTTGACGGGAGTAATCTCGAATTCAACGTCACGGTCGCCCCTACCAATGTGTCCCGCCCTTCTCTGCCAACGCTTACTTACAGAGAGACCAAGATTGCGAACTACGTATCGGCCGAGATGTTCGTTGGGTTTTCGGCTTCGAAAACGCAGTGGATTGAAGCCCAGAGGGTTTTAGCTTGGAGTTTTAGCGACAACGGAGTCGCCAGAGACATTAACACGACAAATCTGCCCGTTTTCCAGCTGGTTTCTCCGTCGACTTCGCTGTCGAGTGGGGCGATTGCCGGGATTTCAATCGGTTGTGTCGTTTTTGTGTTGATCTTAGCATCCGGGTTTTACTTTTACTGGTGGAAGAAAAGGATGGAAGGCGAAGAAACCGACGAGATCGAAGATTGGGAACTGGAGTTTTGGCCGCACAGATTTTCCAACGAGGAGCTGAGGGAGGCGACGGGAGGTTTTTCGAACGATCAGCTTCTGGGTTCCGGCGGGTTCGGAAAGGTTTACAAGGGGACCTTATCAAACGGCACGGAAATCGCCGTCAAGTGTGTCAACCACGACTCGAAGCAGGGGCTGAGGGAGTTCATGGCGGAGATCGCCAGCATGGGGCGGCTGCAGCACAAGCACTTGGTTCAGATGCGGGGCTGGTGCCGGAAGCGGAACGAGCTGATGCTGGTGTATGATTACATGCCGAATGGGAGCCTCAATCGGTGGATTTTCGACAAGCCGAAGAAGGTTTTGGGGTGGGATAGGCGGCGGCGCGTGCTTGCGGATGTGGCGGAGGGGTTGAATTATCTGCACCACGGGTGGGACCAGATGGTGGTTCACAGGGACATTAAGTCAAGCAATATTTTGCTGGACGCGGAGATGAGGGGGCGGCTCGGGGATTTTGGGCTGGCGAAGTTGTACGAGCATGGGGAGGTACCGAATACGACGCGTGTCGTCGGGACGTTGGGCTACTTGGCGCCGGAGCTGGCGACTGTGGCGGCGCCGACGGCGGCGAGTGATGTGTATAGTTTTGGGGTGGTGTTGTTGGAGGTGGCGTGCGGGAGGAAGCCGATTAAGATGGACGCGGCGGAGGATGAGGAGGTGTTGATTGATTGGGTGAGGGAGTTGTATGTTAGAGGGAGGGTGGGGGAGGCAGCGGACCCGAGAATAAAGGGGGAGTATGAGGCGGAGGAGATGGAGATTGCTTTGAAGCTTGGGCTGGCTTGTTGCCATCCTCATCCACAGAGAAGGCCTAACATGAGGGATATTGTGGCGGTTTTGGTTGGTGAGGCCGCAGCCGCCACCGCACCCGCCGCATTGTTGTCTGAATTGGCAAGGGGTGATAGTAGCGTTGGCGGCGGAGACGGCTGTAGTGATGAAGAGTCAATGGAAGCGGCGCCACTCCAACTCCGGCAGCCTTCAGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002229 GO:0002239 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0016020 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0050896 GO:0051704 GO:0051707 GO:0071944 GO:0098542

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Lectin_legB Pkinase

Protein Analysis

701

Amino Acids

77.13

Weight (kDa)

5.67

Isoelectric Point (pI)

37.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Lectin_legB	PF00139	54 - 308	1.2e-65	Legume lectin domain
Pkinase	PF00069	384 - 649	5e-50	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	386 - 651	1.1e-44	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010436)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G15530
fragaria_vesca	FvH4_5g06170
malus_domestica	MD06G1166300.v1.1
prunus_persica	Prupe.5G166300_v2.0.a1 Prupe.5G166300_v2.0.a1
pyrus_communis	pycom06g14890 pycom10g29130 pycom14g14370
rosa_chinensis	RchiOBHm_Chr7g0192631
rosa_laevigata	RLG00000004340 RLG00000004343 RLG00000036720
rosa_multiflora	Rmu_sc0002040.1_g000001 Rmu_sc0035522.1_g000002
rosa_roxburghii	Rroxscaffold_3G00262790 Rroxscaffold_3G00262820
rosa_rugosa	Rorug07G0003200 Rorug07G0003300
rosa_samantha	Rh7BG129900 Rh7DG130900
rosa_wichuraiana	Rw7G010970

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	890, 955
Acc65I	GGTACC	1 cut(s)	1609
AccB1I	GGYRCC	7 cut(s)	69, 239, 1335, 1609, 1651, 1675, 2071
AccB7I	CCANNNNNTGG	1 cut(s)	1507
AccBSI	CCGCTC	2 cut(s)	62, 158
AccI	GTMKAC	1 cut(s)	947
AccII	CGCG	5 cut(s)	288, 1449, 1550, 1624, 1760
AccIII	TCCGGA	1 cut(s)	379
AcoI	YGGCCR	3 cut(s)	283, 804, 1100
AcsI	RAATTY	4 cut(s)	306, 545, 692, 713
AcuI	CTGAAG	1 cut(s)	2079
AcyI	GRCGYC	6 cut(s)	70, 225, 240, 1652, 1676, 2072
AfaI	GTAC	3 cut(s)	623, 1595, 1611
AfiI	CCNNNNNNNGG	6 cut(s)	66, 404, 618, 1293, 1495, 1507
AflIII	ACRYGT	1 cut(s)	1622
AgsI	TTSAA	8 cut(s)	175, 550, 692, 718, 851, 984, 1477, 1885
AhdI	GACNNNNNGTC	1 cut(s)	1525
AhlI	ACTAGT	1 cut(s)	32
AjnI	CCWGG	2 cut(s)	397, 683
AjuI	GAANNNNNNNTTGG	2 cut(s)	1409, 1441
AluBI	AGCT	8 cut(s)	52, 869, 934, 1126, 1162, 1354, 1660, 1888
AluI	AGCT	8 cut(s)	52, 869, 934, 1126, 1162, 1354, 1660, 1888
Alw26I	GTCTC	5 cut(s)	88, 148, 773, 894, 2031
AlwNI	CAGNNNCTG	2 cut(s)	1165, 1261
Ama87I	CYCGRG	3 cut(s)	391, 1568, 1837
Aor13HI	TCCGGA	1 cut(s)	379
AoxI	GGCC	8 cut(s)	283, 406, 469, 681, 804, 1100, 1925, 1965
ApeKI	GCWGC	5 cut(s)	1300, 1303, 1829, 1970, 2089
ApoI	RAATTY	4 cut(s)	306, 545, 692, 713
ArsI	GACNNNNNNTTYG	2 cut(s)	705, 737
Asp718I	GGTACC	1 cut(s)	1609
AspLEI	GCGC	6 cut(s)	72, 242, 1449, 1654, 1678, 2074
AspS9I	GGNCC	6 cut(s)	652, 663, 681, 1200, 1498, 1834
AsuC2I	CCSGG	8 cut(s)	67, 542, 619, 650, 667, 680, 976, 1021
AsuHPI	GGTGA	5 cut(s)	184, 469, 1804, 1973, 2027
AsuII	TTCGAA	2 cut(s)	833, 1151
AsuNHI	GCTAGC	1 cut(s)	511
AvaI	CYCGRG	3 cut(s)	391, 1568, 1837
AvaII	GGWCC	5 cut(s)	652, 663, 1200, 1498, 1834
BanI	GGYRCC	7 cut(s)	69, 239, 1335, 1609, 1651, 1675, 2071
BanII	GRGCYC	1 cut(s)	398
BbsI	GAAGAC	2 cut(s)	283, 358
BbvCI	CCTCAGC	2 cut(s)	1127, 1262
BbvI	GCAGC	4 cut(s)	1287, 1315, 1841, 1982
BccI	CCATC	7 cut(s)	254, 554, 1045, 1498, 1747, 1864, 1914
BceAI	ACGGC	8 cut(s)	92, 253, 263, 500, 1211, 1228, 1698, 2056
BcgI	CGANNNNNNTGC	4 cut(s)	901, 935, 1204, 1238
BciT130I	CCWGG	2 cut(s)	399, 685
BcnI	CCSGG	8 cut(s)	67, 542, 619, 650, 667, 680, 976, 1021
BcoDI	GTCTC	5 cut(s)	88, 148, 773, 894, 2031
BcuI	ACTAGT	1 cut(s)	32
BfaI	CTAG	2 cut(s)	33, 512
BfmI	CTRYAG	2 cut(s)	1301, 2044
BfoI	RGCGCY	5 cut(s)	73, 243, 1655, 1679, 2075
Bme18I	GGWCC	5 cut(s)	652, 663, 1200, 1498, 1834
BmeRI	GACNNNNNGTC	1 cut(s)	1525
BmeT110I	CYCGRG	3 cut(s)	391, 1568, 1837
BmgT120I	GGNCC	6 cut(s)	652, 663, 681, 1200, 1498, 1834
BmsI	GCATC	3 cut(s)	1025, 1314, 1347
BmtI	GCTAGC	1 cut(s)	515
BoxI	GACNNNNGTC	2 cut(s)	531, 1625
BpiI	GAAGAC	2 cut(s)	283, 358
BpmI	CTGGAG	1 cut(s)	1112
Bpu10I	CCTNAGC	2 cut(s)	1127, 1262
Bpu14I	TTCGAA	2 cut(s)	833, 1151
BpuMI	CCSGG	8 cut(s)	67, 542, 619, 650, 667, 680, 976, 1021
BsaAI	YACGTR	1 cut(s)	799
BsaBI	GATNNNNATC	2 cut(s)	564, 983
BsaHI	GRCGYC	6 cut(s)	70, 225, 240, 1652, 1676, 2072
BsaI	GGTCTC	2 cut(s)	88, 773
BsaJI	CCNNGG	3 cut(s)	65, 397, 1489
BsaWI	WCCGGW	2 cut(s)	379, 635
Bsc4I	CCNNNNNNNGG	6 cut(s)	66, 404, 618, 1293, 1495, 1507
Bse1I	ACTGG	3 cut(s)	271, 1040, 1095
Bse3DI	GCAATG	1 cut(s)	384
Bse8I	GATNNNNATC	2 cut(s)	564, 983
BseAI	TCCGGA	1 cut(s)	379
BseBI	CCWGG	2 cut(s)	399, 685
BseDI	CCNNGG	3 cut(s)	65, 397, 1489
BseGI	GGATG	8 cut(s)	96, 246, 1016, 1056, 1465, 1774, 1906, 1912
BseJI	GATNNNNATC	2 cut(s)	564, 983
BseLI	CCNNNNNNNGG	6 cut(s)	66, 404, 618, 1293, 1495, 1507
BseMI	GCAATG	1 cut(s)	384
BseMII	CTCAG	3 cut(s)	117, 1118, 1253
BseNI	ACTGG	3 cut(s)	271, 1040, 1095
BseRI	GAGGAG	6 cut(s)	104, 113, 116, 1136, 1787, 1880
BseX3I	CGGCCG	2 cut(s)	283, 804
BseXI	GCAGC	4 cut(s)	1287, 1315, 1841, 1982
BsgI	GTGCAG	1 cut(s)	1469
Bsh1236I	CGCG	5 cut(s)	288, 1449, 1550, 1624, 1760
Bsh1285I	CGRYCG	4 cut(s)	286, 636, 729, 807
BshFI	GGCC	8 cut(s)	285, 408, 471, 683, 806, 1102, 1927, 1967
BshNI	GGYRCC	7 cut(s)	69, 239, 1335, 1609, 1651, 1675, 2071
BsiEI	CGRYCG	4 cut(s)	286, 636, 729, 807
BsiHKCI	CYCGRG	3 cut(s)	391, 1568, 1837
BslFI	GGGAC	7 cut(s)	476, 632, 730, 1213, 1511, 1532, 1648
BslI	CCNNNNNNNGG	6 cut(s)	66, 404, 618, 1293, 1495, 1507
BsmAI	GTCTC	5 cut(s)	88, 148, 773, 894, 2031
BsmBI	CGTCTC	2 cut(s)	148, 2031
BsmFI	GGGAC	7 cut(s)	476, 632, 730, 1213, 1511, 1532, 1648
BsnI	GGCC	8 cut(s)	285, 408, 471, 683, 806, 1102, 1927, 1967
Bso31I	GGTCTC	2 cut(s)	88, 773
BsoBI	CYCGRG	3 cut(s)	391, 1568, 1837
Bsp119I	TTCGAA	2 cut(s)	833, 1151
Bsp1286I	GDGCHC	1 cut(s)	398
Bsp13I	TCCGGA	1 cut(s)	379
Bsp143I	GATC	4 cut(s)	1008, 1074, 1156, 1281
BspANI	GGCC	8 cut(s)	285, 408, 471, 683, 806, 1102, 1927, 1967
BspCNI	CTCAG	3 cut(s)	116, 1119, 1254
BspEI	TCCGGA	1 cut(s)	379
BspFNI	CGCG	5 cut(s)	288, 1449, 1550, 1624, 1760
BspMAI	CTGCAG	1 cut(s)	1305
BspOI	GCTAGC	1 cut(s)	515
BspT104I	TTCGAA	2 cut(s)	833, 1151
BspT107I	GGYRCC	7 cut(s)	69, 239, 1335, 1609, 1651, 1675, 2071
BspTNI	GGTCTC	2 cut(s)	88, 773
BsrBI	CCGCTC	2 cut(s)	62, 158
BsrDI	GCAATG	1 cut(s)	384
BsrI	ACTGG	3 cut(s)	271, 1040, 1095
BssECI	CCNNGG	3 cut(s)	65, 397, 1489
BssMI	GATC	4 cut(s)	1008, 1074, 1156, 1281
BssNI	GRCGYC	6 cut(s)	70, 225, 240, 1652, 1676, 2072
Bst2UI	CCWGG	2 cut(s)	399, 685
Bst4CI	ACNGT	3 cut(s)	490, 727, 1669
Bst6I	CTCTTC	3 cut(s)	176, 479, 2049
BstACI	GRCGYC	6 cut(s)	70, 225, 240, 1652, 1676, 2072
BstBAI	YACGTR	1 cut(s)	799
BstBI	TTCGAA	2 cut(s)	833, 1151
BstC8I	GCNNGC	9 cut(s)	457, 513, 1288, 1451, 1455, 1584, 1662, 1732, 1896
BstDEI	CTNAG	4 cut(s)	103, 1012, 1127, 1262
BstDSI	CCRYGG	1 cut(s)	1489
BstF5I	GGATG	8 cut(s)	96, 246, 1016, 1056, 1465, 1774, 1906, 1912
BstFNI	CGCG	5 cut(s)	288, 1449, 1550, 1624, 1760
BstH2I	RGCGCY	5 cut(s)	73, 243, 1655, 1679, 2075
BstHHI	GCGC	6 cut(s)	72, 242, 1449, 1654, 1678, 2074
BstKTI	GATC	4 cut(s)	1011, 1077, 1159, 1284
BstMAI	GTCTC	5 cut(s)	88, 148, 773, 894, 2031
BstMBI	GATC	4 cut(s)	1008, 1074, 1156, 1281
BstMCI	CGRYCG	4 cut(s)	286, 636, 729, 807
BstNI	CCWGG	2 cut(s)	399, 685
BstNSI	RCATGY	1 cut(s)	1378
BstPAI	GACNNNNGTC	2 cut(s)	531, 1625
BstSFI	CTRYAG	2 cut(s)	1301, 2044
BstSNI	TACGTA	1 cut(s)	799
BstUI	CGCG	5 cut(s)	288, 1449, 1550, 1624, 1760
BstV1I	GCAGC	4 cut(s)	1287, 1315, 1841, 1982
BstV2I	GAAGAC	2 cut(s)	283, 358
BstZI	CGGCCG	2 cut(s)	283, 804
BsuRI	GGCC	8 cut(s)	285, 408, 471, 683, 806, 1102, 1927, 1967
BtgI	CCRYGG	1 cut(s)	1489
BtsCI	GGATG	8 cut(s)	96, 246, 1016, 1056, 1465, 1774, 1906, 1912
BtsI	GCAGTG	1 cut(s)	848
BtsIMutI	CAGTG	1 cut(s)	848
Cac8I	GCNNGC	9 cut(s)	457, 513, 1288, 1451, 1455, 1584, 1662, 1732, 1896
CaiI	CAGNNNCTG	2 cut(s)	1165, 1261
CfoI	GCGC	6 cut(s)	72, 242, 1449, 1654, 1678, 2074
Cfr13I	GGNCC	6 cut(s)	652, 663, 681, 1200, 1498, 1834
CpoI	CGGWCCG	1 cut(s)	663
CseI	GACGC	5 cut(s)	233, 277, 1556, 1630, 1766
Csp6I	GTAC	3 cut(s)	622, 1594, 1610
CspI	CGGWCCG	1 cut(s)	663
CviAII	CATG	6 cut(s)	313, 1273, 1291, 1375, 1601, 1933
CviQI	GTAC	3 cut(s)	622, 1594, 1610
DdeI	CTNAG	4 cut(s)	103, 1012, 1127, 1262
DinI	GGCGCC	5 cut(s)	71, 241, 1653, 1677, 2073
DpnI	GATC	4 cut(s)	1010, 1076, 1158, 1283
DpnII	GATC	4 cut(s)	1008, 1074, 1156, 1281
DrdI	GACNNNNNNGTC	2 cut(s)	890, 955
DriI	GACNNNNNGTC	1 cut(s)	1525
DseDI	GACNNNNNNGTC	2 cut(s)	890, 955
EaeI	YGGCCR	3 cut(s)	283, 804, 1100
EagI	CGGCCG	2 cut(s)	283, 804
Eam1104I	CTCTTC	3 cut(s)	176, 479, 2049
Eam1105I	GACNNNNNGTC	1 cut(s)	1525
EarI	CTCTTC	3 cut(s)	176, 479, 2049
EciI	GGCGGA	8 cut(s)	31, 239, 589, 1292, 1481, 1778, 1877, 2049
EclXI	CGGCCG	2 cut(s)	283, 804
Eco105I	TACGTA	1 cut(s)	799
Eco147I	AGGCCT	1 cut(s)	1927
Eco24I	GRGCYC	1 cut(s)	398
Eco31I	GGTCTC	2 cut(s)	88, 773
Eco47I	GGWCC	5 cut(s)	652, 663, 1200, 1498, 1834
Eco52I	CGGCCG	2 cut(s)	283, 804
Eco57I	CTGAAG	1 cut(s)	2079
Eco88I	CYCGRG	3 cut(s)	391, 1568, 1837
EcoO109I	RGGNCCY	1 cut(s)	1200
EcoRI	GAATTC	2 cut(s)	545, 713
EcoRII	CCWGG	2 cut(s)	397, 683
EcoT38I	GRGCYC	1 cut(s)	398
EgeI	GGCGCC	5 cut(s)	71, 241, 1653, 1677, 2073
EheI	GGCGCC	5 cut(s)	71, 241, 1653, 1677, 2073
Esp3I	CGTCTC	2 cut(s)	148, 2031
FaeI	CATG	6 cut(s)	316, 1276, 1294, 1378, 1604, 1936
FalI	AAGNNNNNCTT	2 cut(s)	1187, 1219
FaqI	GGGAC	7 cut(s)	476, 632, 730, 1213, 1511, 1532, 1648
FatI	CATG	6 cut(s)	312, 1272, 1290, 1374, 1600, 1932
FauI	CCCGC	7 cut(s)	67, 622, 755, 1169, 1320, 1727, 1993
FblI	GTMKAC	1 cut(s)	947
FokI	GGATG	8 cut(s)	103, 233, 1003, 1063, 1472, 1781, 1893, 1899
FriOI	GRGCYC	1 cut(s)	398
FspBI	CTAG	2 cut(s)	33, 512
GlaI	GCGC	6 cut(s)	71, 241, 1448, 1653, 1677, 2073
GsuI	CTGGAG	1 cut(s)	1112
HaeII	RGCGCY	5 cut(s)	73, 243, 1655, 1679, 2075
HaeIII	GGCC	8 cut(s)	285, 408, 471, 683, 806, 1102, 1927, 1967
HgaI	GACGC	5 cut(s)	233, 277, 1556, 1630, 1766
HhaI	GCGC	6 cut(s)	72, 242, 1449, 1654, 1678, 2074
Hin1I	GRCGYC	6 cut(s)	70, 225, 240, 1652, 1676, 2072
Hin1II	CATG	6 cut(s)	316, 1276, 1294, 1378, 1604, 1936
Hin6I	GCGC	6 cut(s)	70, 240, 1447, 1652, 1676, 2072
HinP1I	GCGC	6 cut(s)	70, 240, 1447, 1652, 1676, 2072
HincII	GTYRAC	2 cut(s)	948, 1240
HindII	GTYRAC	2 cut(s)	948, 1240
HindIII	AAGCTT	2 cut(s)	50, 1886
HphI	GGTGA	5 cut(s)	184, 469, 1804, 1973, 2027
Hpy166II	GTNNAC	4 cut(s)	948, 1192, 1240, 1513
Hpy188I	TCNGA	4 cut(s)	575, 1184, 1323, 2002
Hpy188III	TCNNGA	4 cut(s)	161, 380, 710, 1633
Hpy8I	GTNNAC	4 cut(s)	948, 1192, 1240, 1513
HpyCH4III	ACNGT	3 cut(s)	490, 727, 1669
HpyCH4IV	ACGT	5 cut(s)	591, 675, 720, 798, 1637
HpyCH4V	TGCA	2 cut(s)	1303, 1486
HpyF3I	CTNAG	4 cut(s)	103, 1012, 1127, 1262
HpySE526I	ACGT	5 cut(s)	591, 675, 720, 798, 1637
Hsp92I	GRCGYC	6 cut(s)	70, 225, 240, 1652, 1676, 2072
Hsp92II	CATG	6 cut(s)	316, 1276, 1294, 1378, 1604, 1936
HspAI	GCGC	6 cut(s)	70, 240, 1447, 1652, 1676, 2072
KasI	GGCGCC	5 cut(s)	69, 239, 1651, 1675, 2071
Kpn2I	TCCGGA	1 cut(s)	379
KpnI	GGTACC	1 cut(s)	1613
Kzo9I	GATC	4 cut(s)	1008, 1074, 1156, 1281
LmnI	GCTCC	4 cut(s)	67, 1123, 1386, 1657
Lsp1109I	GCAGC	4 cut(s)	1287, 1315, 1841, 1982
LweI	GCATC	3 cut(s)	1025, 1314, 1347
MaeI	CTAG	2 cut(s)	33, 512
MaeII	ACGT	5 cut(s)	591, 675, 720, 798, 1637
MaeIII	GTNAC	2 cut(s)	35, 721
MalI	GATC	4 cut(s)	1010, 1076, 1158, 1283
MbiI	CCGCTC	2 cut(s)	62, 158
MboI	GATC	4 cut(s)	1008, 1074, 1156, 1281
MboII	GAAGA	9 cut(s)	163, 283, 358, 466, 1054, 1073, 1091, 1429, 2066
MhlI	GDGCHC	1 cut(s)	398
MluCI	AATT	7 cut(s)	306, 445, 545, 692, 713, 1477, 2003
MluI	ACGCGT	1 cut(s)	1622
Mly113I	GGCGCC	5 cut(s)	70, 240, 1652, 1676, 2072
MlyI	GAGTC	4 cut(s)	225, 900, 1241, 2066
MmeI	TCCRAC	5 cut(s)	210, 351, 450, 1140, 1701
MroI	TCCGGA	1 cut(s)	379
MseI	TTAA	5 cut(s)	17, 585, 906, 1524, 1749
MspA1I	CMGCKG	2 cut(s)	934, 1832
MvaI	CCWGG	2 cut(s)	399, 685
MvnI	CGCG	5 cut(s)	288, 1449, 1550, 1624, 1760
NarI	GGCGCC	5 cut(s)	70, 240, 1652, 1676, 2072
NciI	CCSGG	8 cut(s)	67, 542, 619, 650, 667, 680, 976, 1021
NdeII	GATC	4 cut(s)	1008, 1074, 1156, 1281
NheI	GCTAGC	1 cut(s)	511
NlaIII	CATG	6 cut(s)	316, 1276, 1294, 1378, 1604, 1936
NmeAIII	GCCGAG	1 cut(s)	832
NmuCI	GTSAC	2 cut(s)	35, 721
NspI	RCATGY	1 cut(s)	1378
NspV	TTCGAA	2 cut(s)	833, 1151
PaeR7I	CTCGAG	1 cut(s)	391
PceI	AGGCCT	1 cut(s)	1927
PcsI	WCGNNNNNNNCGW	6 cut(s)	231, 369, 717, 804, 965, 993
PfeI	GAWTC	6 cut(s)	80, 402, 565, 576, 596, 642
PflMI	CCANNNNNTGG	1 cut(s)	1507
PleI	GAGTC	4 cut(s)	225, 899, 1241, 2065
PluTI	GGCGCC	5 cut(s)	73, 243, 1655, 1679, 2075
PpsI	GAGTC	4 cut(s)	225, 899, 1241, 2065
Ppu21I	YACGTR	1 cut(s)	799
PpuMI	RGGWCCY	1 cut(s)	1200
PshAI	GACNNNNGTC	2 cut(s)	531, 1625
Psp5II	RGGWCCY	1 cut(s)	1200
Psp6I	CCWGG	2 cut(s)	397, 683
PspGI	CCWGG	2 cut(s)	397, 683
PspPI	GGNCC	6 cut(s)	652, 663, 681, 1200, 1498, 1834
PspPPI	RGGWCCY	1 cut(s)	1200
PspXI	VCTCGAGB	1 cut(s)	391
PstI	CTGCAG	1 cut(s)	1305
PstNI	CAGNNNCTG	2 cut(s)	1165, 1261
PvuII	CAGCTG	1 cut(s)	934
RsaI	GTAC	3 cut(s)	623, 1595, 1611
RsaNI	GTAC	3 cut(s)	622, 1594, 1610
Rsr2I	CGGWCCG	1 cut(s)	663
RsrII	CGGWCCG	1 cut(s)	663
SalI	GTCGAC	1 cut(s)	946
SaqAI	TTAA	5 cut(s)	17, 585, 906, 1524, 1749
Sau3AI	GATC	4 cut(s)	1008, 1074, 1156, 1281
Sau96I	GGNCC	6 cut(s)	652, 663, 681, 1200, 1498, 1834
SchI	GAGTC	4 cut(s)	225, 900, 1241, 2066
SduI	GDGCHC	1 cut(s)	398
SfaNI	GCATC	3 cut(s)	1025, 1314, 1347
SfcI	CTRYAG	2 cut(s)	1301, 2044
SfoI	GGCGCC	5 cut(s)	71, 241, 1653, 1677, 2073
Sfr274I	CTCGAG	1 cut(s)	391
SfuI	TTCGAA	2 cut(s)	833, 1151
SinI	GGWCC	5 cut(s)	652, 663, 1200, 1498, 1834
SlaI	CTCGAG	1 cut(s)	391
SmlI	CTYRAG	1 cut(s)	391
SmoI	CTYRAG	1 cut(s)	391
SnaBI	TACGTA	1 cut(s)	799
SpeI	ACTAGT	1 cut(s)	32
Sse9I	AATT	7 cut(s)	306, 445, 545, 692, 713, 1477, 2003
SseBI	AGGCCT	1 cut(s)	1927
SspDI	GGCGCC	5 cut(s)	69, 239, 1651, 1675, 2071
SspI	AATATT	1 cut(s)	1537
SspMI	CTAG	2 cut(s)	33, 512
StuI	AGGCCT	1 cut(s)	1927
TaaI	ACNGT	3 cut(s)	490, 727, 1669
TaiI	ACGT	5 cut(s)	594, 678, 723, 801, 1640
TasI	AATT	7 cut(s)	306, 445, 545, 692, 713, 1477, 2003
TfiI	GAWTC	6 cut(s)	80, 402, 565, 576, 596, 642
Tru1I	TTAA	5 cut(s)	17, 585, 906, 1524, 1749
Tru9I	TTAA	5 cut(s)	17, 585, 906, 1524, 1749
TscAI	CASTG	1 cut(s)	848
TseFI	GTSAC	2 cut(s)	35, 721
TseI	GCWGC	5 cut(s)	1300, 1303, 1829, 1970, 2089
Tsp45I	GTSAC	2 cut(s)	35, 721
TspDTI	ATGAA	3 cut(s)	329, 1261, 2067
TspGWI	ACGGA	6 cut(s)	103, 328, 361, 903, 933, 1232
TspRI	CASTG	1 cut(s)	848
Van91I	CCANNNNNTGG	1 cut(s)	1507
VpaK11BI	GGWCC	5 cut(s)	652, 663, 1200, 1498, 1834
XapI	RAATTY	4 cut(s)	306, 545, 692, 713
XceI	RCATGY	1 cut(s)	1378
XhoI	CTCGAG	1 cut(s)	391
XmiI	GTMKAC	1 cut(s)	947
XspI	CTAG	2 cut(s)	33, 512

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.