pycom10g29130

L-type lectin-domain containing receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0010436
Physical Location & Seq
Forward (+)
29517135 .. 29517758
624 bp
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UTR
Exon/CDS
Intron
pycom10g29130.1

Sequence Viewer

Length: 624 bp
ATGCGGAAGGATACCCTTCTCCTCCCCTCATTGCTAACTACCCTCCCAAAGACCTGTACCGTCCACCCACCAATCCTCATCAGCCATCCTCATCGTTCTTCTCCTCAACGACCTCTTCCTCGCCCCTACCTCCGCTCTTCAACTCCTTCAAAAAAAATCACTAATGCCATTGACCTCACTCTCATCAACGACGCCCGACTCGACCCAGCCATTCGCCTCACAAATGACACCAACCCGTTCGCCGTCGGCTGTGTCTTCTACCCAACAAAAATTCCCATGAAACCCACCTCCAACTCCTCTTCCGTCGCCACCTTCACCACCTCCTTTGTCTTCTCCGTCCTCCCGGAAATTAAATCGAGCCCGGGATTCGGCCTCTGCTTCGTCCTCTCCAACTCCACCTCGCCAGCCAATACTTTGGCCGCTTCACCAACGCCACCATCCCCTTCGTCGCAGCGTTGCTCGCCGTCGAGTTCCACACGGGTCAAAACCCAGAATTCAACGACCCAAATGGAAATCACATCGAAATTGACGGTGCCGATTGAGCCGTCGGTGAAGGCCTGGCAGAAATCGAAGAGGCCGTCAATTATTGGGCAGTCCTCGCCGACGTTGAAGTGCTTGAGGTGA

Protein Analysis

208

Amino Acids

22.41

Weight (kDa)

10.3

Isoelectric Point (pI)

65.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lectin_legB PF00139 56 - 138 1.6e-15 Legume lectin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 532
AccBSI CCGCTC 1 cut(s) 135
AciI CCGC 3 cut(s) 4, 133, 420
AcoI YGGCCR 1 cut(s) 417
AcsI RAATTY 2 cut(s) 270, 493
AcyI GRCGYC 1 cut(s) 192
AfaI GTAC 1 cut(s) 58
AfiI CCNNNNNNNGG 1 cut(s) 368
AgsI TTSAA 4 cut(s) 141, 150, 498, 610
AjnI CCWGG 1 cut(s) 557
Ama87I CYCGRG 1 cut(s) 361
AoxI GGCC 4 cut(s) 370, 417, 555, 575
ApeKI GCWGC 1 cut(s) 451
ApoI RAATTY 2 cut(s) 270, 493
ArsI GACNNNNNNTTYG 2 cut(s) 563, 595
AsuC2I CCSGG 3 cut(s) 344, 362, 363
AsuHPI GGTGA 3 cut(s) 307, 417, 562
AvaI CYCGRG 1 cut(s) 361
BanI GGYRCC 1 cut(s) 532
BanII GRGCYC 1 cut(s) 362
BbsI GAAGAC 2 cut(s) 247, 322
BbvI GCAGC 1 cut(s) 463
BccI CCATC 2 cut(s) 93, 445
BceAI ACGGC 4 cut(s) 227, 448, 529, 562
BciT130I CCWGG 1 cut(s) 559
BciVI GTATCC 1 cut(s) 4
BcnI CCSGG 3 cut(s) 344, 362, 363
BfuI GTATCC 1 cut(s) 4
BisI GCNGC 2 cut(s) 420, 452
BlsI GCNGC 2 cut(s) 421, 453
Bme1390I CCNGG 4 cut(s) 344, 362, 363, 559
BmeT110I CYCGRG 1 cut(s) 361
BmiI GGNNCC 1 cut(s) 534
BmrFI CCNGG 4 cut(s) 344, 362, 363, 559
BpiI GAAGAC 2 cut(s) 247, 322
BpuMI CCSGG 3 cut(s) 344, 362, 363
BsaHI GRCGYC 1 cut(s) 192
BsaJI CCNNGG 1 cut(s) 361
Bsc4I CCNNNNNNNGG 1 cut(s) 368
Bse3DI GCAATG 1 cut(s) 29
BseBI CCWGG 1 cut(s) 559
BseDI CCNNGG 1 cut(s) 361
BseGI GGATG 2 cut(s) 85, 437
BseLI CCNNNNNNNGG 1 cut(s) 368
BseMI GCAATG 1 cut(s) 29
BseRI GAGGAG 3 cut(s) 11, 93, 286
BseXI GCAGC 1 cut(s) 463
BseYI CCCAGC 1 cut(s) 205
BshFI GGCC 4 cut(s) 372, 419, 557, 577
BshNI GGYRCC 1 cut(s) 532
BsiHKCI CYCGRG 1 cut(s) 361
BsiSI CCGG 2 cut(s) 344, 362
BslI CCNNNNNNNGG 1 cut(s) 368
BsnI GGCC 4 cut(s) 372, 419, 557, 577
BsoBI CYCGRG 1 cut(s) 361
Bsp1286I GDGCHC 1 cut(s) 362
BspACI CCGC 3 cut(s) 4, 133, 420
BspANI GGCC 4 cut(s) 372, 419, 557, 577
BspLI GGNNCC 1 cut(s) 534
BspQI GCTCTTC 1 cut(s) 142
BspT107I GGYRCC 1 cut(s) 532
BsrBI CCGCTC 1 cut(s) 135
BsrDI GCAATG 1 cut(s) 29
BssECI CCNNGG 1 cut(s) 361
BssNI GRCGYC 1 cut(s) 192
Bst2UI CCWGG 1 cut(s) 559
Bst4CI ACNGT 2 cut(s) 61, 532
Bst6I CTCTTC 4 cut(s) 120, 142, 304, 566
BstACI GRCGYC 1 cut(s) 192
BstC8I GCNNGC 2 cut(s) 405, 461
BstF5I GGATG 2 cut(s) 85, 437
BstMWI GCNNNNNNNGC 3 cut(s) 460, 541, 598
BstNI CCWGG 1 cut(s) 559
BstSCI CCNGG 4 cut(s) 342, 360, 361, 557
BstV1I GCAGC 1 cut(s) 463
BstV2I GAAGAC 2 cut(s) 247, 322
BstXI CCANNNNNNTGG 1 cut(s) 415
BsuI GTATCC 1 cut(s) 4
BsuRI GGCC 4 cut(s) 372, 419, 557, 577
BtsCI GGATG 2 cut(s) 85, 437
Cac8I GCNNGC 2 cut(s) 405, 461
Cfr9I CCCGGG 1 cut(s) 361
CseI GACGC 1 cut(s) 200
Csp6I GTAC 1 cut(s) 57
CspCI CAANNNNNGTGG 2 cut(s) 307, 342
CviAII CATG 1 cut(s) 277
CviQI GTAC 1 cut(s) 57
EaeI YGGCCR 1 cut(s) 417
Eam1104I CTCTTC 4 cut(s) 120, 142, 304, 566
EarI CTCTTC 4 cut(s) 120, 142, 304, 566
Eco147I AGGCCT 1 cut(s) 557
Eco24I GRGCYC 1 cut(s) 362
Eco88I CYCGRG 1 cut(s) 361
EcoRI GAATTC 1 cut(s) 493
EcoRII CCWGG 1 cut(s) 557
EcoT38I GRGCYC 1 cut(s) 362
FaeI CATG 1 cut(s) 280
FaiI YATR 1 cut(s) 278
FatI CATG 1 cut(s) 276
Fnu4HI GCNGC 2 cut(s) 420, 452
FokI GGATG 2 cut(s) 72, 424
FriOI GRGCYC 1 cut(s) 362
Fsp4HI GCNGC 2 cut(s) 420, 452
GluI GCNGC 2 cut(s) 420, 452
GsaI CCCAGC 1 cut(s) 209
HaeIII GGCC 4 cut(s) 372, 419, 557, 577
HapII CCGG 2 cut(s) 344, 362
HgaI GACGC 1 cut(s) 200
Hin1I GRCGYC 1 cut(s) 192
Hin1II CATG 1 cut(s) 280
HinfI GANTC 2 cut(s) 198, 366
HpaII CCGG 2 cut(s) 344, 362
HphI GGTGA 3 cut(s) 307, 417, 562
Hpy166II GTNNAC 1 cut(s) 64
Hpy8I GTNNAC 1 cut(s) 64
Hpy99I CGWCG 7 cut(s) 194, 248, 308, 451, 469, 550, 607
HpyAV CCTTC 5 cut(s) 26, 156, 322, 453, 547
HpyCH4III ACNGT 2 cut(s) 61, 532
HpyCH4IV ACGT 1 cut(s) 605
HpyF10VI GCNNNNNNNGC 3 cut(s) 460, 541, 598
HpySE526I ACGT 1 cut(s) 605
Hsp92I GRCGYC 1 cut(s) 192
Hsp92II CATG 1 cut(s) 280
LguI GCTCTTC 1 cut(s) 142
LpnPI CCDG 8 cut(s) 67, 219, 357, 375, 417, 503, 544, 571
Lsp1109I GCAGC 1 cut(s) 463
MaeII ACGT 1 cut(s) 605
MbiI CCGCTC 1 cut(s) 135
MboII GAAGA 7 cut(s) 90, 107, 129, 247, 291, 322, 583
MhlI GDGCHC 1 cut(s) 362
MluCI AATT 5 cut(s) 270, 348, 493, 524, 582
MlyI GAGTC 1 cut(s) 192
MmeI TCCRAC 2 cut(s) 315, 414
MseI TTAA 1 cut(s) 351
MspI CCGG 2 cut(s) 344, 362
MspR9I CCNGG 4 cut(s) 344, 362, 363, 559
MvaI CCWGG 1 cut(s) 559
MwoI GCNNNNNNNGC 3 cut(s) 460, 541, 598
NciI CCSGG 3 cut(s) 344, 362, 363
NlaIII CATG 1 cut(s) 280
NlaIV GGNNCC 1 cut(s) 534
PceI AGGCCT 1 cut(s) 557
PciSI GCTCTTC 1 cut(s) 142
PcsI WCGNNNNNNNCGW 2 cut(s) 198, 575
PfeI GAWTC 1 cut(s) 366
PfoI TCCNGGA 1 cut(s) 342
PkrI GCNGC 2 cut(s) 421, 453
PleI GAGTC 1 cut(s) 192
PpsI GAGTC 1 cut(s) 192
Psp6I CCWGG 1 cut(s) 557
PspFI CCCAGC 1 cut(s) 205
PspGI CCWGG 1 cut(s) 557
PspN4I GGNNCC 1 cut(s) 534
RsaI GTAC 1 cut(s) 58
RsaNI GTAC 1 cut(s) 57
SapI GCTCTTC 1 cut(s) 142
SaqAI TTAA 1 cut(s) 351
SatI GCNGC 2 cut(s) 420, 452
SchI GAGTC 1 cut(s) 192
ScrFI CCNGG 4 cut(s) 344, 362, 363, 559
SduI GDGCHC 1 cut(s) 362
SmaI CCCGGG 1 cut(s) 363
SmlI CTYRAG 1 cut(s) 616
SmoI CTYRAG 1 cut(s) 616
Sse9I AATT 5 cut(s) 270, 348, 493, 524, 582
SseBI AGGCCT 1 cut(s) 557
SsiI CCGC 3 cut(s) 4, 133, 420
StuI AGGCCT 1 cut(s) 557
StyD4I CCNGG 4 cut(s) 342, 360, 361, 557
TaaI ACNGT 2 cut(s) 61, 532
TaiI ACGT 1 cut(s) 608
TaqI TCGA 5 cut(s) 201, 356, 467, 521, 569
TasI AATT 5 cut(s) 270, 348, 493, 524, 582
TauI GCSGC 1 cut(s) 422
TfiI GAWTC 1 cut(s) 366
Tru1I TTAA 1 cut(s) 351
Tru9I TTAA 1 cut(s) 351
TseI GCWGC 1 cut(s) 451
TspDTI ATGAA 1 cut(s) 293
TspGWI ACGGA 2 cut(s) 292, 325
TspMI CCCGGG 1 cut(s) 361
XapI RAATTY 2 cut(s) 270, 493
XmaI CCCGGG 1 cut(s) 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.