RLG00000036720

L-type lectin-domain containing receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0010436
Physical Location & Seq
Forward (+)
83737796 .. 83738554
759 bp
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UTR
Exon/CDS
Intron
RLM00000036720

Sequence Viewer

Length: 576 bp
ATGGCTATGGTTAATCTACCACGGCCTTCTCTGCCTACGCTGAACTTTAAAGACCCAATAATTGCGAACTACGTGTCATCGGAGATGCTTGTCGGGTTTTTAGCATCAAAAACGGAGTGGATTGAGGCGCAGAGAGTTTTGGCTTGGAATTTCAGTCACAATGGAGTTGCTAAAGAGATCAATATGGCCAATTTGCCTGTTTTCGAGCTCAAGTTTCTTCGAAATCCTTGTCTAAGGGAGCCATTGCTGGGATCACAGTCGGCTTATGGAGTTTACTTGTGTTCGTGGAAGAAGAAGCTGGAGGATGAAGAAGATGATGAGATTGAGGATTGGGAGATGAAGTATTGGCCGTATCAATTTTCGAATGAAGAGCTGAGTGAGACTACTGATGGCTCTTTGAAAGATCACCTTTTGGGTTCTAGTGGATTTGGGAAGGTTTACAGAGGAATTTTATTGAACAAGACGAAGATAGTAGTGAAATGTGTGAACCATGATTCAAAGCAGGGGTTGAGTGAATTCATGGTTGAGATTTCGAGCATGGGGAGGCTGCAGCATAAGAATTTGGTCCAAATGTGA

Protein Analysis

192

Amino Acids

21.88

Weight (kDa)

5.63

Isoelectric Point (pI)

42.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 136 - 191 1.3e-07 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 259
AcoI YGGCCR 2 cut(s) 186, 347
AcsI RAATTY 4 cut(s) 148, 447, 515, 559
AfiI CCNNNNNNNGG 1 cut(s) 248
AflIII ACRYGT 1 cut(s) 72
AgsI TTSAA 3 cut(s) 400, 457, 498
AluBI AGCT 3 cut(s) 208, 298, 373
AluI AGCT 3 cut(s) 208, 298, 373
Alw21I GWGCWC 1 cut(s) 210
Alw26I GTCTC 1 cut(s) 374
AlwI GGATC 1 cut(s) 259
AoxI GGCC 3 cut(s) 23, 186, 347
ApeKI GCWGC 2 cut(s) 547, 550
ApoI RAATTY 4 cut(s) 148, 447, 515, 559
ArsI GACNNNNNNTTYG 4 cut(s) 59, 91, 214, 246
AspLEI GCGC 1 cut(s) 130
AspS9I GGNCC 1 cut(s) 565
AsuHPI GGTGA 1 cut(s) 398
AsuII TTCGAA 2 cut(s) 220, 362
AvaII GGWCC 1 cut(s) 565
BalI TGGCCA 1 cut(s) 188
BanII GRGCYC 1 cut(s) 210
Bbv12I GWGCWC 1 cut(s) 210
BbvI GCAGC 2 cut(s) 534, 562
BccI CCATC 1 cut(s) 383
BceAI ACGGC 2 cut(s) 38, 334
BcoDI GTCTC 1 cut(s) 374
BfaI CTAG 1 cut(s) 420
BfmI CTRYAG 1 cut(s) 548
BisI GCNGC 2 cut(s) 548, 551
BlsI GCNGC 2 cut(s) 549, 552
Bme18I GGWCC 1 cut(s) 565
BmgT120I GGNCC 1 cut(s) 565
BmiI GGNNCC 1 cut(s) 240
BmsI GCATC 2 cut(s) 75, 113
BpmI CTGGAG 1 cut(s) 320
Bpu14I TTCGAA 2 cut(s) 220, 362
BpuEI CTTGAG 1 cut(s) 194
BsaAI YACGTR 1 cut(s) 73
BsaJI CCNNGG 1 cut(s) 20
BsaXI ACNNNNNCTCC 2 cut(s) 326, 356
Bsc4I CCNNNNNNNGG 1 cut(s) 248
Bse3DI GCAATG 1 cut(s) 242
BseDI CCNNGG 1 cut(s) 20
BseGI GGATG 1 cut(s) 310
BseLI CCNNNNNNNGG 1 cut(s) 248
BseMI GCAATG 1 cut(s) 242
BseMII CTCAG 1 cut(s) 365
BseXI GCAGC 2 cut(s) 534, 562
BseYI CCCAGC 1 cut(s) 247
BshFI GGCC 3 cut(s) 25, 188, 349
BsiHKAI GWGCWC 1 cut(s) 210
BslI CCNNNNNNNGG 1 cut(s) 248
BsmAI GTCTC 1 cut(s) 374
BsnI GGCC 3 cut(s) 25, 188, 349
Bsp119I TTCGAA 2 cut(s) 220, 362
Bsp1286I GDGCHC 1 cut(s) 210
Bsp143I GATC 3 cut(s) 177, 251, 403
BspANI GGCC 3 cut(s) 25, 188, 349
BspCNI CTCAG 1 cut(s) 366
BspLI GGNNCC 1 cut(s) 240
BspMAI CTGCAG 1 cut(s) 552
BspPI GGATC 1 cut(s) 259
BspQI GCTCTTC 1 cut(s) 363
BspT104I TTCGAA 2 cut(s) 220, 362
BsrDI GCAATG 1 cut(s) 242
BssECI CCNNGG 1 cut(s) 20
BssMI GATC 3 cut(s) 177, 251, 403
Bst4CI ACNGT 1 cut(s) 258
Bst6I CTCTTC 1 cut(s) 363
BstBAI YACGTR 1 cut(s) 73
BstBI TTCGAA 2 cut(s) 220, 362
BstDEI CTNAG 2 cut(s) 233, 374
BstDSI CCRYGG 1 cut(s) 20
BstF5I GGATG 1 cut(s) 310
BstHHI GCGC 1 cut(s) 130
BstKTI GATC 3 cut(s) 180, 254, 406
BstMAI GTCTC 1 cut(s) 374
BstMBI GATC 3 cut(s) 177, 251, 403
BstMWI GCNNNNNNNGC 1 cut(s) 31
BstSFI CTRYAG 1 cut(s) 548
BstV1I GCAGC 2 cut(s) 534, 562
BsuRI GGCC 3 cut(s) 25, 188, 349
BtgI CCRYGG 1 cut(s) 20
BtsCI GGATG 1 cut(s) 310
CfoI GCGC 1 cut(s) 130
Cfr13I GGNCC 1 cut(s) 565
CviAII CATG 3 cut(s) 491, 520, 538
DdeI CTNAG 2 cut(s) 233, 374
DpnI GATC 3 cut(s) 179, 253, 405
DpnII GATC 3 cut(s) 177, 251, 403
DraI TTTAAA 1 cut(s) 49
EaeI YGGCCR 2 cut(s) 186, 347
Eam1104I CTCTTC 1 cut(s) 363
EarI CTCTTC 1 cut(s) 363
Ecl136II GAGCTC 1 cut(s) 208
Eco24I GRGCYC 1 cut(s) 210
Eco47I GGWCC 1 cut(s) 565
Eco53kI GAGCTC 1 cut(s) 208
EcoICRI GAGCTC 1 cut(s) 208
EcoRI GAATTC 1 cut(s) 515
EcoT38I GRGCYC 1 cut(s) 210
FaeI CATG 3 cut(s) 494, 523, 541
FaiI YATR 7 cut(s) 8, 185, 267, 492, 521, 539, 555
FalI AAGNNNNNCTT 2 cut(s) 393, 425
FatI CATG 3 cut(s) 490, 519, 537
Fnu4HI GCNGC 2 cut(s) 548, 551
FokI GGATG 1 cut(s) 317
FriOI GRGCYC 1 cut(s) 210
Fsp4HI GCNGC 2 cut(s) 548, 551
FspBI CTAG 1 cut(s) 420
GlaI GCGC 1 cut(s) 129
GluI GCNGC 2 cut(s) 548, 551
GsaI CCCAGC 1 cut(s) 251
GsuI CTGGAG 1 cut(s) 320
HaeIII GGCC 3 cut(s) 25, 188, 349
HhaI GCGC 1 cut(s) 130
Hin1II CATG 3 cut(s) 494, 523, 541
Hin6I GCGC 1 cut(s) 128
HinP1I GCGC 1 cut(s) 128
HinfI GANTC 1 cut(s) 494
HphI GGTGA 1 cut(s) 398
Hpy166II GTNNAC 3 cut(s) 274, 439, 487
Hpy188I TCNGA 1 cut(s) 82
Hpy8I GTNNAC 3 cut(s) 274, 439, 487
HpyAV CCTTC 2 cut(s) 36, 427
HpyCH4III ACNGT 1 cut(s) 258
HpyCH4IV ACGT 1 cut(s) 72
HpyCH4V TGCA 1 cut(s) 550
HpyF10VI GCNNNNNNNGC 1 cut(s) 31
HpyF3I CTNAG 2 cut(s) 233, 374
HpySE526I ACGT 1 cut(s) 72
Hsp92II CATG 3 cut(s) 494, 523, 541
HspAI GCGC 1 cut(s) 128
Kzo9I GATC 3 cut(s) 177, 251, 403
LguI GCTCTTC 1 cut(s) 363
LmnI GCTCC 1 cut(s) 238
LpnPI CCDG 4 cut(s) 210, 233, 284, 488
Lsp1109I GCAGC 2 cut(s) 534, 562
LweI GCATC 2 cut(s) 75, 113
MaeI CTAG 1 cut(s) 420
MaeII ACGT 1 cut(s) 72
MaeIII GTNAC 1 cut(s) 155
MalI GATC 3 cut(s) 179, 253, 405
MboI GATC 3 cut(s) 177, 251, 403
MboII GAAGA 7 cut(s) 209, 301, 304, 320, 323, 380, 478
MhlI GDGCHC 1 cut(s) 210
MlsI TGGCCA 1 cut(s) 188
MluCI AATT 7 cut(s) 60, 148, 190, 356, 447, 515, 559
MluNI TGGCCA 1 cut(s) 188
MnlI CCTC 5 cut(s) 118, 295, 319, 437, 537
Mox20I TGGCCA 1 cut(s) 188
MscI TGGCCA 1 cut(s) 188
MseI TTAA 2 cut(s) 12, 48
Msp20I TGGCCA 1 cut(s) 188
MwoI GCNNNNNNNGC 1 cut(s) 31
NdeII GATC 3 cut(s) 177, 251, 403
NlaIII CATG 3 cut(s) 494, 523, 541
NlaIV GGNNCC 1 cut(s) 240
NmuCI GTSAC 1 cut(s) 155
NspV TTCGAA 2 cut(s) 220, 362
PciSI GCTCTTC 1 cut(s) 363
PfeI GAWTC 1 cut(s) 494
PkrI GCNGC 2 cut(s) 549, 552
Ppu21I YACGTR 1 cut(s) 73
Psp124BI GAGCTC 1 cut(s) 210
PspFI CCCAGC 1 cut(s) 247
PspN4I GGNNCC 1 cut(s) 240
PspPI GGNCC 1 cut(s) 565
PstI CTGCAG 1 cut(s) 552
SacI GAGCTC 1 cut(s) 210
SapI GCTCTTC 1 cut(s) 363
SaqAI TTAA 2 cut(s) 12, 48
SatI GCNGC 2 cut(s) 548, 551
Sau3AI GATC 3 cut(s) 177, 251, 403
Sau96I GGNCC 1 cut(s) 565
SduI GDGCHC 1 cut(s) 210
SetI ASST 6 cut(s) 75, 210, 300, 375, 411, 438
SfaNI GCATC 2 cut(s) 75, 113
SfcI CTRYAG 1 cut(s) 548
SfuI TTCGAA 2 cut(s) 220, 362
SinI GGWCC 1 cut(s) 565
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
Sse9I AATT 7 cut(s) 60, 148, 190, 356, 447, 515, 559
SspMI CTAG 1 cut(s) 420
SstI GAGCTC 1 cut(s) 210
TaaI ACNGT 1 cut(s) 258
TaiI ACGT 1 cut(s) 75
TaqI TCGA 4 cut(s) 204, 220, 362, 533
TasI AATT 7 cut(s) 60, 148, 190, 356, 447, 515, 559
TfiI GAWTC 1 cut(s) 494
Tru1I TTAA 2 cut(s) 12, 48
Tru9I TTAA 2 cut(s) 12, 48
TseFI GTSAC 1 cut(s) 155
TseI GCWGC 2 cut(s) 547, 550
Tsp45I GTSAC 1 cut(s) 155
TspDTI ATGAA 4 cut(s) 321, 353, 381, 508
TspGWI ACGGA 1 cut(s) 128
VpaK11BI GGWCC 1 cut(s) 565
XapI RAATTY 4 cut(s) 148, 447, 515, 559
XspI CTAG 1 cut(s) 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.