Rorug07G0003200

L-type lectin-domain containing receptor kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0010436
Physical Location & Seq
Forward (+)
297163 .. 300290
3128 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0003200.1

Sequence Viewer

Length: 780 bp
ATGAGCAAGGCCTCCATTTCCAAGGAGCTCAACGACAAGCACACCAAGATTTTAGAAGGTCTTCTTAAGATGCCAGAAAACCGGGAATGTGCTGATTGTCGGAGCAAAGCCCCACGATGGGCAAGTGTGAATCTGGGAATATTTATTTGCATGCAATGTTCAGGAATTCATCGGAGCCTTGGAGTGCACATTTCAAAGGTCCGGTCTACTACTTTGGATACATGGCTGCCAGAGCAGATTGCTTTTATGGAATCTTTGGGTAATGAAAAGGCAAACAATTATTGGGAAAAAAAGTTGCCACTAAATGCAGACAGAAGTGCGATCGAGAAGTTTATTCGTGCCAAGTATGAGGGGAAAAAGTGGGTTGCAAAGGATGCAAAACAGCCCGCTCCTGAACCAGTTGAAATGGGCACCAGTTCTAACAAGTTGGTAGAAGGTGGAGCCACCAGTGGCAAGAAACTAAGGAGGCATTCTCTTGAGGAAGCAATACTTCCTACACATCTTTCAGAGGATGCTCCTCCAGTAGCAAGACCCCGCACGGGTTCTTTAGATTTGAATCGCATTTATACTAATTCACCTTTACCAAAGGGGTCAGCACCGATGACAGATTGCGATACAGCAACAAGAAAGAACAACAGTGCAACAGATCTCTTCAATTTGCTTTATGTCCATGATGCAAAACAGGAATGTCATACTGTACCTCCTGCACGCTGGGCAACTTTTGATTCTGCTGACATTAAAGTCAGAGTGGTAGCTGAAGTGGGCAATGTTTGTTCCTAG

Protein Analysis

259

Amino Acids

28.7

Weight (kDa)

9.01

Isoelectric Point (pI)

54.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 16 - 124 4e-43 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 740
AccB1I GGYRCC 1 cut(s) 410
AccBSI CCGCTC 1 cut(s) 389
AccI GTMKAC 1 cut(s) 206
AciI CCGC 2 cut(s) 387, 535
AcsI RAATTY 1 cut(s) 165
AcuI CTGAAG 1 cut(s) 777
AfaI GTAC 1 cut(s) 699
AfiI CCNNNNNNNGG 3 cut(s) 117, 118, 539
AflII CTTAAG 1 cut(s) 65
AgsI TTSAA 4 cut(s) 195, 404, 556, 655
AluBI AGCT 2 cut(s) 28, 755
AluI AGCT 2 cut(s) 28, 755
Alw21I GWGCWC 2 cut(s) 30, 189
Alw44I GTGCAC 1 cut(s) 185
AoxI GGCC 1 cut(s) 9
ApaLI GTGCAC 1 cut(s) 185
ApeKI GCWGC 1 cut(s) 226
ApoI RAATTY 1 cut(s) 165
ArsI GACNNNNNNTTYG 2 cut(s) 188, 220
Asp700I GAANNNNTTC 1 cut(s) 60
AspS9I GGNCC 1 cut(s) 199
AsuC2I CCSGG 1 cut(s) 83
AsuHPI GGTGA 1 cut(s) 567
AvaII GGWCC 1 cut(s) 199
BaeGI GKGCMC 2 cut(s) 189, 413
BanI GGYRCC 1 cut(s) 410
BanII GRGCYC 1 cut(s) 30
BbsI GAAGAC 1 cut(s) 53
Bbv12I GWGCWC 2 cut(s) 30, 189
BbvI GCAGC 1 cut(s) 213
BccI CCATC 1 cut(s) 111
BciVI GTATCC 1 cut(s) 211
BcnI CCSGG 1 cut(s) 83
BfaI CTAG 1 cut(s) 778
BfrI CTTAAG 1 cut(s) 65
BfuI GTATCC 1 cut(s) 211
BglII AGATCT 1 cut(s) 646
BisI GCNGC 1 cut(s) 227
BlsI GCNGC 1 cut(s) 228
Bme1390I CCNGG 1 cut(s) 83
Bme18I GGWCC 1 cut(s) 199
BmgT120I GGNCC 1 cut(s) 199
BmiI GGNNCC 3 cut(s) 176, 412, 442
BmrFI CCNGG 1 cut(s) 83
BmsI GCATC 4 cut(s) 60, 364, 502, 664
BpiI GAAGAC 1 cut(s) 53
BplI GAGNNNNNCTC 2 cut(s) 457, 489
BpmI CTGGAG 1 cut(s) 504
BpuEI CTTGAG 1 cut(s) 497
BpuMI CCSGG 1 cut(s) 83
BsaBI GATNNNNATC 1 cut(s) 555
BsaJI CCNNGG 2 cut(s) 21, 178
BsaWI WCCGGW 1 cut(s) 201
BsaXI ACNNNNNCTCC 2 cut(s) 685, 715
Bsc4I CCNNNNNNNGG 3 cut(s) 117, 118, 539
Bse1I ACTGG 4 cut(s) 398, 414, 447, 521
Bse3DI GCAATG 2 cut(s) 161, 772
Bse8I GATNNNNATC 1 cut(s) 555
BseDI CCNNGG 2 cut(s) 21, 178
BseGI GGATG 2 cut(s) 379, 517
BseJI GATNNNNATC 1 cut(s) 555
BseLI CCNNNNNNNGG 3 cut(s) 117, 118, 539
BseMI GCAATG 2 cut(s) 161, 772
BseNI ACTGG 4 cut(s) 398, 414, 447, 521
BseRI GAGGAG 1 cut(s) 507
BseSI GKGCMC 2 cut(s) 189, 413
BseXI GCAGC 1 cut(s) 213
BseYI CCCAGC 1 cut(s) 711
BsgI GTGCAG 1 cut(s) 690
Bsh1285I CGRYCG 1 cut(s) 324
BshFI GGCC 1 cut(s) 11
BshNI GGYRCC 1 cut(s) 410
BsiEI CGRYCG 1 cut(s) 324
BsiHKAI GWGCWC 2 cut(s) 30, 189
BsiSI CCGG 2 cut(s) 82, 202
BslI CCNNNNNNNGG 3 cut(s) 117, 118, 539
BsmI GAATGC 1 cut(s) 469
BsnI GGCC 1 cut(s) 11
Bsp1286I GDGCHC 3 cut(s) 30, 189, 413
Bsp143I GATC 2 cut(s) 321, 646
BspACI CCGC 2 cut(s) 387, 535
BspANI GGCC 1 cut(s) 11
BspLI GGNNCC 3 cut(s) 176, 412, 442
BspT107I GGYRCC 1 cut(s) 410
BspTI CTTAAG 1 cut(s) 65
BsrBI CCGCTC 1 cut(s) 389
BsrDI GCAATG 2 cut(s) 161, 772
BsrI ACTGG 4 cut(s) 398, 414, 447, 521
BssECI CCNNGG 2 cut(s) 21, 178
BssMI GATC 2 cut(s) 321, 646
BssT1I CCWWGG 2 cut(s) 21, 178
Bst4CI ACNGT 2 cut(s) 638, 697
Bst6I CTCTTC 1 cut(s) 656
BstAFI CTTAAG 1 cut(s) 65
BstAPI GCANNNNNTGC 1 cut(s) 374
BstC8I GCNNGC 3 cut(s) 152, 387, 709
BstDEI CTNAG 1 cut(s) 461
BstF5I GGATG 2 cut(s) 379, 517
BstKTI GATC 2 cut(s) 324, 649
BstMBI GATC 2 cut(s) 321, 646
BstMCI CGRYCG 1 cut(s) 324
BstMWI GCNNNNNNNGC 3 cut(s) 232, 374, 713
BstNSI RCATGY 1 cut(s) 154
BstSCI CCNGG 1 cut(s) 81
BstSLI GKGCMC 2 cut(s) 189, 413
BstV1I GCAGC 1 cut(s) 213
BstV2I GAAGAC 1 cut(s) 53
BstX2I RGATCY 1 cut(s) 646
BstYI RGATCY 1 cut(s) 646
BsuI GTATCC 1 cut(s) 211
BsuRI GGCC 1 cut(s) 11
BtsCI GGATG 2 cut(s) 379, 517
BtsIMutI CAGTG 2 cut(s) 454, 643
Cac8I GCNNGC 3 cut(s) 152, 387, 709
Cfr13I GGNCC 1 cut(s) 199
Csp6I GTAC 1 cut(s) 698
CviAII CATG 3 cut(s) 151, 222, 671
CviJI RGCY 8 cut(s) 11, 28, 110, 177, 226, 385, 443, 755
CviKI_1 RGCY 8 cut(s) 11, 28, 110, 177, 226, 385, 443, 755
CviQI GTAC 1 cut(s) 698
DdeI CTNAG 1 cut(s) 461
DpnI GATC 2 cut(s) 323, 648
DpnII GATC 2 cut(s) 321, 646
DrdI GACNNNNNNGTC 1 cut(s) 740
DseDI GACNNNNNNGTC 1 cut(s) 740
Eam1104I CTCTTC 1 cut(s) 656
EarI CTCTTC 1 cut(s) 656
Ecl136II GAGCTC 1 cut(s) 28
Eco130I CCWWGG 2 cut(s) 21, 178
Eco147I AGGCCT 1 cut(s) 11
Eco24I GRGCYC 1 cut(s) 30
Eco47I GGWCC 1 cut(s) 199
Eco53kI GAGCTC 1 cut(s) 28
Eco57I CTGAAG 1 cut(s) 777
EcoICRI GAGCTC 1 cut(s) 28
EcoRI GAATTC 1 cut(s) 165
EcoT14I CCWWGG 2 cut(s) 21, 178
EcoT38I GRGCYC 1 cut(s) 30
ErhI CCWWGG 2 cut(s) 21, 178
FaeI CATG 3 cut(s) 154, 225, 674
FaiI YATR 8 cut(s) 152, 223, 248, 348, 567, 666, 672, 693
FalI AAGNNNNNCTT 4 cut(s) 48, 80, 474, 506
FatI CATG 3 cut(s) 150, 221, 670
FauI CCCGC 2 cut(s) 394, 542
FblI GTMKAC 1 cut(s) 206
Fnu4HI GCNGC 1 cut(s) 227
FokI GGATG 2 cut(s) 386, 524
FriOI GRGCYC 1 cut(s) 30
Fsp4HI GCNGC 1 cut(s) 227
FspBI CTAG 1 cut(s) 778
GluI GCNGC 1 cut(s) 227
GsaI CCCAGC 1 cut(s) 715
GsuI CTGGAG 1 cut(s) 504
HaeIII GGCC 1 cut(s) 11
HapII CCGG 2 cut(s) 82, 202
Hin1II CATG 3 cut(s) 154, 225, 674
HinfI GANTC 4 cut(s) 130, 251, 556, 725
HpaII CCGG 2 cut(s) 82, 202
HphI GGTGA 1 cut(s) 567
Hpy166II GTNNAC 2 cut(s) 187, 207
Hpy188I TCNGA 4 cut(s) 102, 174, 508, 746
Hpy188III TCNNGA 4 cut(s) 162, 325, 392, 476
Hpy8I GTNNAC 2 cut(s) 187, 207
HpyAV CCTTC 2 cut(s) 50, 428
HpyCH4III ACNGT 2 cut(s) 638, 697
HpyCH4V TGCA 9 cut(s) 150, 154, 187, 308, 368, 377, 641, 677, 707
HpyF10VI GCNNNNNNNGC 3 cut(s) 232, 374, 713
HpyF3I CTNAG 1 cut(s) 461
Hsp92II CATG 3 cut(s) 154, 225, 674
Kzo9I GATC 2 cut(s) 321, 646
LmnI GCTCC 6 cut(s) 25, 102, 174, 394, 440, 520
Lsp1109I GCAGC 1 cut(s) 213
LweI GCATC 4 cut(s) 60, 364, 502, 664
MaeI CTAG 1 cut(s) 778
MalI GATC 2 cut(s) 323, 648
MbiI CCGCTC 1 cut(s) 389
MboI GATC 2 cut(s) 321, 646
MboII GAAGA 2 cut(s) 53, 643
MflI RGATCY 1 cut(s) 646
MhlI GDGCHC 3 cut(s) 30, 189, 413
MluCI AATT 4 cut(s) 165, 277, 571, 655
MmeI TCCRAC 1 cut(s) 80
MnlI CCTC 7 cut(s) 22, 343, 459, 472, 502, 528, 711
MroXI GAANNNNTTC 1 cut(s) 60
MseI TTAA 2 cut(s) 66, 738
MspCI CTTAAG 1 cut(s) 65
MspI CCGG 2 cut(s) 82, 202
MspR9I CCNGG 1 cut(s) 83
Mva1269I GAATGC 1 cut(s) 469
MwoI GCNNNNNNNGC 3 cut(s) 232, 374, 713
NciI CCSGG 1 cut(s) 83
NdeII GATC 2 cut(s) 321, 646
NlaIII CATG 3 cut(s) 154, 225, 674
NlaIV GGNNCC 3 cut(s) 176, 412, 442
NspI RCATGY 1 cut(s) 154
PaeI GCATGC 1 cut(s) 154
PceI AGGCCT 1 cut(s) 11
PctI GAATGC 1 cut(s) 469
PdmI GAANNNNTTC 1 cut(s) 60
PfeI GAWTC 4 cut(s) 130, 251, 556, 725
PkrI GCNGC 1 cut(s) 228
Ple19I CGATCG 1 cut(s) 324
Psp124BI GAGCTC 1 cut(s) 30
PspFI CCCAGC 1 cut(s) 711
PspN4I GGNNCC 3 cut(s) 176, 412, 442
PspPI GGNCC 1 cut(s) 199
PsuI RGATCY 1 cut(s) 646
PvuI CGATCG 1 cut(s) 324
RsaI GTAC 1 cut(s) 699
RsaNI GTAC 1 cut(s) 698
SacI GAGCTC 1 cut(s) 30
SaqAI TTAA 2 cut(s) 66, 738
SatI GCNGC 1 cut(s) 227
Sau3AI GATC 2 cut(s) 321, 646
Sau96I GGNCC 1 cut(s) 199
ScrFI CCNGG 1 cut(s) 83
SduI GDGCHC 3 cut(s) 30, 189, 413
SetI ASST 7 cut(s) 30, 61, 201, 439, 580, 703, 757
SfaNI GCATC 4 cut(s) 60, 364, 502, 664
SinI GGWCC 1 cut(s) 199
SmlI CTYRAG 2 cut(s) 65, 476
SmoI CTYRAG 2 cut(s) 65, 476
SphI GCATGC 1 cut(s) 154
Sse9I AATT 4 cut(s) 165, 277, 571, 655
SseBI AGGCCT 1 cut(s) 11
SsiI CCGC 2 cut(s) 387, 535
SspI AATATT 1 cut(s) 141
SspMI CTAG 1 cut(s) 778
SstI GAGCTC 1 cut(s) 30
StuI AGGCCT 1 cut(s) 11
StyD4I CCNGG 1 cut(s) 81
StyI CCWWGG 2 cut(s) 21, 178
TaaI ACNGT 2 cut(s) 638, 697
TaqI TCGA 1 cut(s) 324
TasI AATT 4 cut(s) 165, 277, 571, 655
TfiI GAWTC 4 cut(s) 130, 251, 556, 725
Tru1I TTAA 2 cut(s) 66, 738
Tru9I TTAA 2 cut(s) 66, 738
TscAI CASTG 2 cut(s) 454, 643
TseI GCWGC 1 cut(s) 226
TspDTI ATGAA 2 cut(s) 158, 279
TspRI CASTG 2 cut(s) 454, 643
Vha464I CTTAAG 1 cut(s) 65
VneI GTGCAC 1 cut(s) 185
VpaK11BI GGWCC 1 cut(s) 199
XapI RAATTY 1 cut(s) 165
XceI RCATGY 1 cut(s) 154
XmiI GTMKAC 1 cut(s) 206
XmnI GAANNNNTTC 1 cut(s) 60
XspI CTAG 1 cut(s) 778
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.