MD06G1170400.v1.1

Neprosin activation peptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0011754
Physical Location & Seq
Reverse (-)
31113930 .. 31118272
4343 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1170400.v1.1.491

Sequence Viewer

Length: 1518 bp
ATGAAGGGAGTAGGAGAAGAAGGTTTTAATCCTAATCCACCACAACTTCCATGGACAAAACTGAGGAATGTGATATCCCACTACACAACAACAACTAGACAACCAGTCCAATGCAACCAAACACAAATCTCTGATTCCGTTTTTGTACCAAACAGTAGACAGAGACAACAGCAACAACAACCTACTCCCCTAATCAAACCCAAACATTCCTCCAAACTGGCTCCTCCTACTTATTCTCACACCTCCACCTCCTTCTCATTCGTCTCCATGCTTCTTGTTCGCTTACTTCTTGTTGCTTCTTCTTCTCTCTTTACTCCTGCATTGTCCCACCCAGTTGTCTCGGATTCCGGCCTACGCCGCCCCTCCCCGGCCAATCGGACATTTCGGACCAGCTCGGAGATCAACAAGTTGAGGGGGGTCCAAGCCCAATCATATTTAAGGAAAATCAACAAGCCTGCTGTCAAGACTATTCAGAGTCCAGATGGCGATGTGATAGATTGTGTTTTATCTCATCTCCAACCAGCTTTTGATCATCCTGAGCTCAAAGGGAAGAAGCCATTGGACGAACCAGACAGGCCCAAAAACCATAACAGCACAGATAAAGTAGGTGAGAGCTATCAGCTATGGACAGACTCAGGTGAATCATGTCCACAAGGAACAGTACCGATCCGGCGCACCGGAGAGAAAGACATTTTACGCGCAAGCTCCATCAGAAGATTCGGCAGAAAACCTAGAAGACACGTGAGGAGAGACTCCTCTGGCAGTGGCCATGAGCATGCAGTTGTGTTTGTAAATGGAGATCAGTACTACGGAGCAAAGGCCAGCATCAATGTGTGGGCGCCACGAGTCACTGATCAGTATGAATTTAGCTTGTCCCAGATTTGGGTTATCTCTGGTTCTTTTGGCCATGATCTCAACACCATTGAAGCTGGATGGCAGGTTAGCCCGGAGTTGTATGGAGATAATTACCCAAGATTCTTTACATACTGGACAACAGATGCATATCAAGCAACTGGATGCTACAACTTGCTTTGCTCTGGATTTGTCCAAACAAATAATAAAATTGCAATTGGAGCAGCAATCTCCCCAAGGTCCTCTTATGGTAGCAGACAATTTGACATTGGCTTGATGGTCTGGAAGGATCCAAGGCATGGACATTGGTGGCTAGAATTTGGAAATGGCCTACTGGTTGGATACTGGCCAGCATTCTTGTTCAGTCACCTGAGGAGCCATGCAAGCATGGTGCAATTTGGGGGTGAAATTGTAAATAGCAGATCTAGAGGGTACCACACATCTACACAAATGGGGAGTGGACAATTTGCAGAAGCAGGGTTTGGAAAAGCATCTTATTTCAGAAACTTGCAGGTTGTGGATTGGGATAACAATTTGCTCCCACTCGCAAATCTCCACCTCCAGGCTGATCACCCTAATTGTTATGATATAAGACAGGGAAGAAATAATGTGTGGGGGACTTACTTTTACTATGGAGGTCCTGGAAGAAATGTGAGGTGTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

506

Amino Acids

56.79

Weight (kDa)

9.55

Isoelectric Point (pI)

46.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin_AP PF14365 156 - 262 1.7e-37 Neprosin activation peptide
Neprosin PF03080 287 - 498 1.4e-84 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011754)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50150
fragaria_vesca FvH4_5g05740
malus_domestica MD06G1170400.v1.1 MD14G1176000.v1.1
prunus_persica Prupe.5G170000_v2.0.a1 Prupe.5G170000_v2.0.a1
pyrus_communis pycom06g15180 pycom14g14710
rosa_chinensis RchiOBHm_Chr7g0193071
rosa_laevigata RLG00000004297
rosa_multiflora Rmu_ssc0000002.1_g000010
rosa_roxburghii Rroxscaffold_3G00262400
rosa_rugosa Rorug07G0007400
rosa_samantha Rh7AG132600 Rh7BG133500 Rh7CG136600 Rh7DG134500
rosa_wichuraiana Rw0G015850 Rw7G011330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 928, 1354
Acc65I GGTACC 1 cut(s) 1284
AccB1I GGYRCC 2 cut(s) 838, 1284
AccB7I CCANNNNNTGG 1 cut(s) 1151
AccI GTMKAC 1 cut(s) 157
AccII CGCG 1 cut(s) 699
AciI CCGC 1 cut(s) 358
AclWI GGATC 3 cut(s) 661, 1136, 1149
AcoI YGGCCR 4 cut(s) 369, 766, 904, 1199
AcsI RAATTY 2 cut(s) 863, 1169
AcvI CACGTG 1 cut(s) 742
AcyI GRCGYC 1 cut(s) 839
AfaI GTAC 4 cut(s) 147, 663, 806, 1286
AfiI CCNNNNNNNGG 5 cut(s) 367, 882, 883, 1151, 1414
AflIII ACRYGT 1 cut(s) 739
AgsI TTSAA 1 cut(s) 926
AhdI GACNNNNNGTC 1 cut(s) 104
AjnI CCWGG 2 cut(s) 1413, 1492
AjuI GAANNNNNNNTTGG 4 cut(s) 542, 574, 1317, 1349
AluBI AGCT 8 cut(s) 393, 524, 541, 615, 622, 705, 870, 929
AluI AGCT 8 cut(s) 393, 524, 541, 615, 622, 705, 870, 929
Alw21I GWGCWC 1 cut(s) 543
Alw26I GTCTC 4 cut(s) 157, 268, 343, 744
AlwI GGATC 3 cut(s) 661, 1136, 1149
AoxI GGCC 8 cut(s) 349, 369, 575, 766, 819, 904, 1180, 1199
ApeKI GCWGC 1 cut(s) 1076
ApoI RAATTY 2 cut(s) 863, 1169
Asp718I GGTACC 1 cut(s) 1284
AspLEI GCGC 3 cut(s) 675, 701, 841
AspS9I GGNCC 5 cut(s) 387, 418, 576, 1092, 1490
AsuC2I CCSGG 2 cut(s) 368, 947
AsuHPI GGTGA 5 cut(s) 620, 650, 1211, 1268, 1415
AvaII GGWCC 4 cut(s) 387, 418, 1092, 1490
AxyI CCTNAGG 1 cut(s) 1223
BalI TGGCCA 3 cut(s) 768, 906, 1201
BamHI GGATCC 1 cut(s) 1141
BanI GGYRCC 2 cut(s) 838, 1284
BanII GRGCYC 1 cut(s) 543
BauI CACGAG 1 cut(s) 843
BbrPI CACGTG 1 cut(s) 742
BbsI GAAGAC 1 cut(s) 742
Bbv12I GWGCWC 1 cut(s) 543
BbvI GCAGC 1 cut(s) 1088
BccI CCATC 4 cut(s) 476, 716, 927, 1123
BciT130I CCWGG 2 cut(s) 1415, 1494
BciVI GTATCC 1 cut(s) 1187
BclI TGATCA 3 cut(s) 529, 853, 1420
BcnI CCSGG 2 cut(s) 368, 947
BcoDI GTCTC 4 cut(s) 157, 268, 343, 744
BfaI CTAG 4 cut(s) 96, 732, 1166, 1278
BfoI RGCGCY 1 cut(s) 842
BfuAI ACCTGC 2 cut(s) 928, 1354
BfuI GTATCC 1 cut(s) 1187
BglII AGATCT 1 cut(s) 1274
BisI GCNGC 2 cut(s) 358, 1077
BlsI GCNGC 2 cut(s) 359, 1078
BmcAI AGTACT 1 cut(s) 806
Bme1390I CCNGG 4 cut(s) 368, 947, 1415, 1494
Bme18I GGWCC 4 cut(s) 387, 418, 1092, 1490
BmeRI GACNNNNNGTC 1 cut(s) 104
BmgT120I GGNCC 5 cut(s) 387, 418, 576, 1092, 1490
BmiI GGNNCC 6 cut(s) 222, 419, 840, 1143, 1229, 1286
BmrFI CCNGG 4 cut(s) 368, 947, 1415, 1494
BmrI ACTGGG 1 cut(s) 326
BmsI GCATC 4 cut(s) 834, 988, 1007, 1352
BmuI ACTGGG 1 cut(s) 326
BpiI GAAGAC 1 cut(s) 742
BpmI CTGGAG 1 cut(s) 1397
Bpu10I CCTNAGC 1 cut(s) 537
BpuMI CCSGG 2 cut(s) 368, 947
BsaAI YACGTR 1 cut(s) 742
BsaBI GATNNNNATC 1 cut(s) 1002
BsaHI GRCGYC 1 cut(s) 839
BsaJI CCNNGG 4 cut(s) 50, 366, 1088, 1145
BsaWI WCCGGW 1 cut(s) 677
Bsc4I CCNNNNNNNGG 5 cut(s) 367, 882, 883, 1151, 1414
Bse1I ACTGG 7 cut(s) 104, 222, 332, 992, 1018, 1191, 1202
Bse21I CCTNAGG 1 cut(s) 1223
Bse8I GATNNNNATC 1 cut(s) 1002
BseBI CCWGG 2 cut(s) 1415, 1494
BseDI CCNNGG 4 cut(s) 50, 366, 1088, 1145
BseGI GGATG 3 cut(s) 532, 938, 1022
BseJI GATNNNNATC 1 cut(s) 1002
BseLI CCNNNNNNNGG 5 cut(s) 367, 882, 883, 1151, 1414
BseMII CTCAG 4 cut(s) 53, 528, 648, 1214
BseNI ACTGG 7 cut(s) 104, 222, 332, 992, 1018, 1191, 1202
BseRI GAGGAG 4 cut(s) 213, 745, 760, 1240
BseXI GCAGC 1 cut(s) 1088
Bsh1236I CGCG 1 cut(s) 699
BshFI GGCC 8 cut(s) 351, 371, 577, 768, 821, 906, 1182, 1201
BshNI GGYRCC 2 cut(s) 838, 1284
BsiHKAI GWGCWC 1 cut(s) 543
BsiSI CCGG 5 cut(s) 348, 368, 670, 678, 947
BslFI GGGAC 3 cut(s) 310, 859, 1483
BslI CCNNNNNNNGG 5 cut(s) 367, 882, 883, 1151, 1414
BsmAI GTCTC 4 cut(s) 157, 268, 343, 744
BsmBI CGTCTC 1 cut(s) 268
BsmFI GGGAC 3 cut(s) 310, 859, 1483
BsmI GAATGC 1 cut(s) 1205
BsnI GGCC 8 cut(s) 351, 371, 577, 768, 821, 906, 1182, 1201
Bsp1286I GDGCHC 1 cut(s) 543
Bsp143I GATC 9 cut(s) 399, 529, 666, 799, 853, 910, 1141, 1274, 1420
Bsp19I CCATGG 1 cut(s) 50
BspACI CCGC 1 cut(s) 358
BspANI GGCC 8 cut(s) 351, 371, 577, 768, 821, 906, 1182, 1201
BspCNI CTCAG 4 cut(s) 54, 529, 647, 1215
BspFNI CGCG 1 cut(s) 699
BspLI GGNNCC 6 cut(s) 222, 419, 840, 1143, 1229, 1286
BspMI ACCTGC 2 cut(s) 928, 1354
BspPI GGATC 3 cut(s) 661, 1136, 1149
BspT107I GGYRCC 2 cut(s) 838, 1284
BsrI ACTGG 7 cut(s) 104, 222, 332, 992, 1018, 1191, 1202
BssECI CCNNGG 4 cut(s) 50, 366, 1088, 1145
BssMI GATC 9 cut(s) 399, 529, 666, 799, 853, 910, 1141, 1274, 1420
BssNI GRCGYC 1 cut(s) 839
BssSI CACGAG 1 cut(s) 843
BssT1I CCWWGG 3 cut(s) 50, 1088, 1145
Bst2BI CACGAG 1 cut(s) 843
Bst2UI CCWGG 2 cut(s) 1415, 1494
Bst4CI ACNGT 2 cut(s) 155, 661
BstACI GRCGYC 1 cut(s) 839
BstBAI YACGTR 1 cut(s) 742
BstC8I GCNNGC 6 cut(s) 456, 703, 777, 823, 1203, 1237
BstDEI CTNAG 4 cut(s) 62, 537, 634, 1223
BstDSI CCRYGG 1 cut(s) 50
BstF5I GGATG 3 cut(s) 532, 938, 1022
BstFNI CGCG 1 cut(s) 699
BstH2I RGCGCY 1 cut(s) 842
BstHHI GCGC 3 cut(s) 675, 701, 841
BstKTI GATC 9 cut(s) 402, 532, 669, 802, 856, 913, 1144, 1277, 1423
BstMAI GTCTC 4 cut(s) 157, 268, 343, 744
BstMBI GATC 9 cut(s) 399, 529, 666, 799, 853, 910, 1141, 1274, 1420
BstMWI GCNNNNNNNGC 4 cut(s) 357, 1007, 1073, 1236
BstNI CCWGG 2 cut(s) 1415, 1494
BstNSI RCATGY 1 cut(s) 779
BstSCI CCNGG 4 cut(s) 366, 945, 1413, 1492
BstUI CGCG 1 cut(s) 699
BstV1I GCAGC 1 cut(s) 1088
BstV2I GAAGAC 1 cut(s) 742
BstX2I RGATCY 2 cut(s) 1141, 1274
BstYI RGATCY 2 cut(s) 1141, 1274
Bsu36I CCTNAGG 1 cut(s) 1223
BsuI GTATCC 1 cut(s) 1187
BsuRI GGCC 8 cut(s) 351, 371, 577, 768, 821, 906, 1182, 1201
BtgI CCRYGG 1 cut(s) 50
BtgZI GCGATG 1 cut(s) 501
BtsCI GGATG 3 cut(s) 532, 938, 1022
BtsI GCAGTG 1 cut(s) 769
BtsIMutI CAGTG 2 cut(s) 769, 849
BveI ACCTGC 2 cut(s) 928, 1354
Cac8I GCNNGC 6 cut(s) 456, 703, 777, 823, 1203, 1237
CfoI GCGC 3 cut(s) 675, 701, 841
Cfr13I GGNCC 5 cut(s) 387, 418, 576, 1092, 1490
Csp6I GTAC 4 cut(s) 146, 662, 805, 1285
CviQI GTAC 4 cut(s) 146, 662, 805, 1285
DdeI CTNAG 4 cut(s) 62, 537, 634, 1223
DinI GGCGCC 1 cut(s) 840
DpnI GATC 9 cut(s) 401, 531, 668, 801, 855, 912, 1143, 1276, 1422
DpnII GATC 9 cut(s) 399, 529, 666, 799, 853, 910, 1141, 1274, 1420
DriI GACNNNNNGTC 1 cut(s) 104
EaeI YGGCCR 4 cut(s) 369, 766, 904, 1199
Eam1105I GACNNNNNGTC 1 cut(s) 104
Ecl136II GAGCTC 1 cut(s) 541
Eco130I CCWWGG 3 cut(s) 50, 1088, 1145
Eco24I GRGCYC 1 cut(s) 543
Eco32I GATATC 1 cut(s) 75
Eco47I GGWCC 4 cut(s) 387, 418, 1092, 1490
Eco53kI GAGCTC 1 cut(s) 541
Eco72I CACGTG 1 cut(s) 742
Eco81I CCTNAGG 1 cut(s) 1223
EcoICRI GAGCTC 1 cut(s) 541
EcoO109I RGGNCCY 2 cut(s) 1092, 1490
EcoRII CCWGG 2 cut(s) 1413, 1492
EcoRV GATATC 1 cut(s) 75
EcoT14I CCWWGG 3 cut(s) 50, 1088, 1145
EcoT22I ATGCAT 1 cut(s) 1003
EcoT38I GRGCYC 1 cut(s) 543
EgeI GGCGCC 1 cut(s) 840
EheI GGCGCC 1 cut(s) 840
ErhI CCWWGG 3 cut(s) 50, 1088, 1145
Esp3I CGTCTC 1 cut(s) 268
FalI AAGNNNNNCTT 2 cut(s) 1081, 1113
FaqI GGGAC 3 cut(s) 310, 859, 1483
FbaI TGATCA 3 cut(s) 529, 853, 1420
FblI GTMKAC 1 cut(s) 157
Fnu4HI GCNGC 2 cut(s) 358, 1077
FokI GGATG 3 cut(s) 519, 945, 1029
FriOI GRGCYC 1 cut(s) 543
Fsp4HI GCNGC 2 cut(s) 358, 1077
FspBI CTAG 4 cut(s) 96, 732, 1166, 1278
GlaI GCGC 3 cut(s) 674, 700, 840
GluI GCNGC 2 cut(s) 358, 1077
GsuI CTGGAG 1 cut(s) 1397
HaeII RGCGCY 1 cut(s) 842
HaeIII GGCC 8 cut(s) 351, 371, 577, 768, 821, 906, 1182, 1201
HapII CCGG 5 cut(s) 348, 368, 670, 678, 947
HhaI GCGC 3 cut(s) 675, 701, 841
Hin1I GRCGYC 1 cut(s) 839
Hin6I GCGC 3 cut(s) 673, 699, 839
HinP1I GCGC 3 cut(s) 673, 699, 839
HinfI GANTC 9 cut(s) 134, 344, 475, 632, 641, 717, 752, 846, 975
HpaII CCGG 5 cut(s) 348, 368, 670, 678, 947
HphI GGTGA 5 cut(s) 620, 650, 1211, 1268, 1415
Hpy166II GTNNAC 3 cut(s) 158, 650, 1313
Hpy188I TCNGA 8 cut(s) 133, 343, 378, 387, 397, 474, 713, 1355
Hpy188III TCNNGA 6 cut(s) 463, 479, 536, 1038, 1135, 1278
Hpy8I GTNNAC 3 cut(s) 158, 650, 1313
HpyAV CCTTC 3 cut(s) 14, 262, 1132
HpyCH4III ACNGT 2 cut(s) 155, 661
HpyCH4IV ACGT 1 cut(s) 741
HpyCH4V TGCA 9 cut(s) 114, 320, 779, 1001, 1067, 1235, 1246, 1322, 1363
HpyF10VI GCNNNNNNNGC 4 cut(s) 357, 1007, 1073, 1236
HpyF3I CTNAG 4 cut(s) 62, 537, 634, 1223
HpySE526I ACGT 1 cut(s) 741
Hsp92I GRCGYC 1 cut(s) 839
HspAI GCGC 3 cut(s) 673, 699, 839
KasI GGCGCC 1 cut(s) 838
KpnI GGTACC 1 cut(s) 1288
Ksp22I TGATCA 3 cut(s) 529, 853, 1420
Kzo9I GATC 9 cut(s) 399, 529, 666, 799, 853, 910, 1141, 1274, 1420
LmnI GCTCC 6 cut(s) 226, 710, 812, 1073, 1227, 1395
Lsp1109I GCAGC 1 cut(s) 1088
LweI GCATC 4 cut(s) 834, 988, 1007, 1352
MaeI CTAG 4 cut(s) 96, 732, 1166, 1278
MaeII ACGT 1 cut(s) 741
MaeIII GTNAC 2 cut(s) 847, 1217
MalI GATC 9 cut(s) 401, 531, 668, 801, 855, 912, 1143, 1276, 1422
MboI GATC 9 cut(s) 399, 529, 666, 799, 853, 910, 1141, 1274, 1420
MboII GAAGA 8 cut(s) 29, 291, 294, 562, 726, 747, 1464, 1509
MfeI CAATTG 1 cut(s) 1068
MflI RGATCY 2 cut(s) 1141, 1274
MhlI GDGCHC 1 cut(s) 543
MlsI TGGCCA 3 cut(s) 768, 906, 1201
MluNI TGGCCA 3 cut(s) 768, 906, 1201
Mly113I GGCGCC 1 cut(s) 839
MlyI GAGTC 4 cut(s) 484, 626, 746, 855
MmeI TCCRAC 2 cut(s) 541, 1171
Mox20I TGGCCA 3 cut(s) 768, 906, 1201
Mph1103I ATGCAT 1 cut(s) 1003
MscI TGGCCA 3 cut(s) 768, 906, 1201
MseI TTAA 2 cut(s) 27, 437
MslI CAYNNNNRTG 2 cut(s) 774, 830
Msp20I TGGCCA 3 cut(s) 768, 906, 1201
MspI CCGG 5 cut(s) 348, 368, 670, 678, 947
MspR9I CCNGG 4 cut(s) 368, 947, 1415, 1494
MunI CAATTG 1 cut(s) 1068
Mva1269I GAATGC 1 cut(s) 1205
MvaI CCWGG 2 cut(s) 1415, 1494
MvnI CGCG 1 cut(s) 699
MwoI GCNNNNNNNGC 4 cut(s) 357, 1007, 1073, 1236
NarI GGCGCC 1 cut(s) 839
NciI CCSGG 2 cut(s) 368, 947
NcoI CCATGG 1 cut(s) 50
NdeII GATC 9 cut(s) 399, 529, 666, 799, 853, 910, 1141, 1274, 1420
NlaIV GGNNCC 6 cut(s) 222, 419, 840, 1143, 1229, 1286
NmuCI GTSAC 2 cut(s) 847, 1217
NsiI ATGCAT 1 cut(s) 1003
NspI RCATGY 1 cut(s) 779
PaeI GCATGC 1 cut(s) 779
PctI GAATGC 1 cut(s) 1205
PfeI GAWTC 5 cut(s) 134, 344, 641, 717, 975
PflMI CCANNNNNTGG 1 cut(s) 1151
PfoI TCCNGGA 1 cut(s) 1492
PkrI GCNGC 2 cut(s) 359, 1078
PleI GAGTC 4 cut(s) 483, 626, 746, 854
PluTI GGCGCC 1 cut(s) 842
PmaCI CACGTG 1 cut(s) 742
PmlI CACGTG 1 cut(s) 742
PpsI GAGTC 4 cut(s) 483, 626, 746, 854
Ppu21I YACGTR 1 cut(s) 742
PpuMI RGGWCCY 2 cut(s) 1092, 1490
Psp124BI GAGCTC 1 cut(s) 543
Psp5II RGGWCCY 2 cut(s) 1092, 1490
Psp6I CCWGG 2 cut(s) 1413, 1492
PspCI CACGTG 1 cut(s) 742
PspGI CCWGG 2 cut(s) 1413, 1492
PspN4I GGNNCC 6 cut(s) 222, 419, 840, 1143, 1229, 1286
PspPI GGNCC 5 cut(s) 387, 418, 576, 1092, 1490
PspPPI RGGWCCY 2 cut(s) 1092, 1490
PsuI RGATCY 2 cut(s) 1141, 1274
RsaI GTAC 4 cut(s) 147, 663, 806, 1286
RsaNI GTAC 4 cut(s) 146, 662, 805, 1285
RseI CAYNNNNRTG 2 cut(s) 774, 830
SacI GAGCTC 1 cut(s) 543
SaqAI TTAA 2 cut(s) 27, 437
SatI GCNGC 2 cut(s) 358, 1077
Sau3AI GATC 9 cut(s) 399, 529, 666, 799, 853, 910, 1141, 1274, 1420
Sau96I GGNCC 5 cut(s) 387, 418, 576, 1092, 1490
ScaI AGTACT 1 cut(s) 806
SchI GAGTC 4 cut(s) 484, 626, 746, 855
ScrFI CCNGG 4 cut(s) 368, 947, 1415, 1494
SduI GDGCHC 1 cut(s) 543
SfaNI GCATC 4 cut(s) 834, 988, 1007, 1352
SfoI GGCGCC 1 cut(s) 840
SinI GGWCC 4 cut(s) 387, 418, 1092, 1490
SmiMI CAYNNNNRTG 2 cut(s) 774, 830
SphI GCATGC 1 cut(s) 779
SsiI CCGC 1 cut(s) 358
SspDI GGCGCC 1 cut(s) 838
SspMI CTAG 4 cut(s) 96, 732, 1166, 1278
SstI GAGCTC 1 cut(s) 543
StyD4I CCNGG 4 cut(s) 366, 945, 1413, 1492
StyI CCWWGG 3 cut(s) 50, 1088, 1145
TaaI ACNGT 2 cut(s) 155, 661
TaiI ACGT 1 cut(s) 744
TatI WGTACW 1 cut(s) 804
TauI GCSGC 1 cut(s) 360
TfiI GAWTC 5 cut(s) 134, 344, 641, 717, 975
Tru1I TTAA 2 cut(s) 27, 437
Tru9I TTAA 2 cut(s) 27, 437
TscAI CASTG 2 cut(s) 769, 856
TseFI GTSAC 2 cut(s) 847, 1217
TseI GCWGC 1 cut(s) 1076
Tsp45I GTSAC 2 cut(s) 847, 1217
TspDTI ATGAA 2 cut(s) 17, 876
TspGWI ACGGA 2 cut(s) 127, 825
TspRI CASTG 2 cut(s) 769, 856
Van91I CCANNNNNTGG 1 cut(s) 1151
VpaK11BI GGWCC 4 cut(s) 387, 418, 1092, 1490
XapI RAATTY 2 cut(s) 863, 1169
XbaI TCTAGA 1 cut(s) 1277
XceI RCATGY 1 cut(s) 779
XcmI CCANNNNNNNNNTGG 1 cut(s) 48
XmiI GTMKAC 1 cut(s) 157
XspI CTAG 4 cut(s) 96, 732, 1166, 1278
ZrmI AGTACT 1 cut(s) 806
Zsp2I ATGCAT 1 cut(s) 1003
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.