Rw0G015850

Neprosin activation peptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00742
OG0011754
Physical Location & Seq
Forward (+)
37496 .. 38732
1237 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G015850.1

Sequence Viewer

Length: 885 bp
ATGAAAGGAGCTTATTCAGCTGTTCTTGTTGGTTCTGCTCTGTCGACAAAAGAGAGGAATATTGAAGCAGAATCATCCTCATCATCTTCTTGTGCAATTCAGCACACAACAGGACAAGAGCAATGCATACAAGCACGTACTGCTGAAACATTACAAACAACTCCACTCAAACATTCCTCTAACCTGGTTGCTCATACTTTCCACAGCACCCCTTTCTCATTCACATCCATGCTTCTTGTTCTCTTACTTCTTGTTGCTTTTGCTCCTGGACTTTCCTCCCTCCCCATTGCCTCGGATTCCGGGCACCGCCCACCGGCCAATCAGACATTCCGGCCTGATGGGGAAGAGGTGCAGAACTTGAAGAGGGTCAGCTCTTATTTAAGCAAGATCAACAAGCCTGCTGTCAAGACTATTAAGAGTCCTGATGGTGATGTGATAGACTGTGTTTTAGCCAATCTCCAACCAGCTTTTGACCATCCTGAGCTTAAAGGCCAAAAACCATTGGATCTACCAGAAAGGCCTAAATCCCATAACAGCACGGATATAGTGGAAGAGACTTATCAGCTATGGTCGGAATCCGGTGAGGCATGCCCGGAGGGAACTGTTCCGATTCGGAGGACCACAGACAAAGACATTTTGAGAGCAAGTTCAGTTCAAAGATTTGGAAGGAAATTACCAAGAAGACATGTGAAAAGGGACTCATCAGGCAGTGGTCATGAGCATGCAGTTGTTTTTGTAAATGGAGACCAGTACTATGGAGCAAAGGCCAGTATAAATGTGTGGTCGCCAAGAGTGACTGATCAATATGAATTTAGCTTATCACAGATTTGGGTCATCTCTGGTACATTTGGCCATGACCTAAATACCATTGAAGCTGGTTGGCAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

295

Amino Acids

32.35

Weight (kDa)

6.83

Isoelectric Point (pI)

49.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin_AP PF14365 137 - 244 1.4e-37 Neprosin activation peptide
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011754)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50150
fragaria_vesca FvH4_5g05740
malus_domestica MD06G1170400.v1.1 MD14G1176000.v1.1
prunus_persica Prupe.5G170000_v2.0.a1 Prupe.5G170000_v2.0.a1
pyrus_communis pycom06g15180 pycom14g14710
rosa_chinensis RchiOBHm_Chr7g0193071
rosa_laevigata RLG00000004297
rosa_multiflora Rmu_ssc0000002.1_g000010
rosa_roxburghii Rroxscaffold_3G00262400
rosa_rugosa Rorug07G0007400
rosa_samantha Rh7AG132600 Rh7BG133500 Rh7CG136600 Rh7DG134500
rosa_wichuraiana Rw0G015850 Rw7G011330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 303
AccI GTMKAC 1 cut(s) 44
AciI CCGC 1 cut(s) 307
AclWI GGATC 1 cut(s) 513
AcoI YGGCCR 2 cut(s) 315, 850
AcsI RAATTY 1 cut(s) 809
AfaI GTAC 3 cut(s) 139, 752, 844
AfiI CCNNNNNNNGG 1 cut(s) 313
AflIII ACRYGT 1 cut(s) 685
AgsI TTSAA 4 cut(s) 65, 361, 656, 872
AjnI CCWGG 2 cut(s) 183, 265
AjuI GAANNNNNNNTTGG 2 cut(s) 311, 343
AluBI AGCT 8 cut(s) 11, 20, 372, 467, 484, 565, 816, 875
AluI AGCT 8 cut(s) 11, 20, 372, 467, 484, 565, 816, 875
Alw26I GTCTC 2 cut(s) 548, 738
AlwI GGATC 1 cut(s) 513
AoxI GGCC 6 cut(s) 315, 332, 490, 518, 765, 850
ApoI RAATTY 1 cut(s) 809
AspS9I GGNCC 1 cut(s) 618
AsuC2I CCSGG 2 cut(s) 301, 593
AsuHPI GGTGA 2 cut(s) 440, 593
AvaII GGWCC 1 cut(s) 618
BaeGI GKGCMC 1 cut(s) 306
BalI TGGCCA 1 cut(s) 852
BanI GGYRCC 1 cut(s) 303
BbsI GAAGAC 1 cut(s) 688
BccI CCATC 3 cut(s) 332, 419, 483
BciT130I CCWGG 2 cut(s) 185, 267
BclI TGATCA 1 cut(s) 799
BcnI CCSGG 2 cut(s) 301, 593
BcoDI GTCTC 2 cut(s) 548, 738
BmcAI AGTACT 1 cut(s) 752
Bme1390I CCNGG 4 cut(s) 185, 267, 301, 593
Bme18I GGWCC 1 cut(s) 618
BmgT120I GGNCC 1 cut(s) 618
BmiI GGNNCC 1 cut(s) 305
BmrFI CCNGG 4 cut(s) 185, 267, 301, 593
BpiI GAAGAC 1 cut(s) 688
Bpu10I CCTNAGC 1 cut(s) 480
BpuMI CCSGG 2 cut(s) 301, 593
BsaAI YACGTR 1 cut(s) 137
BsaI GGTCTC 1 cut(s) 738
BsaJI CCNNGG 1 cut(s) 291
BsaWI WCCGGW 1 cut(s) 578
Bsc4I CCNNNNNNNGG 1 cut(s) 313
Bse118I RCCGGY 1 cut(s) 313
Bse1I ACTGG 2 cut(s) 748, 768
Bse3DI GCAATG 2 cut(s) 128, 285
BseBI CCWGG 2 cut(s) 185, 267
BseDI CCNNGG 1 cut(s) 291
BseGI GGATG 3 cut(s) 74, 224, 475
BseLI CCNNNNNNNGG 1 cut(s) 313
BseMI GCAATG 2 cut(s) 128, 285
BseMII CTCAG 1 cut(s) 471
BseNI ACTGG 2 cut(s) 748, 768
BseSI GKGCMC 1 cut(s) 306
BsgI GTGCAG 1 cut(s) 371
BshFI GGCC 6 cut(s) 317, 334, 492, 520, 767, 852
BshNI GGYRCC 1 cut(s) 303
BsiSI CCGG 5 cut(s) 300, 314, 331, 579, 593
BslFI GGGAC 1 cut(s) 710
BslI CCNNNNNNNGG 1 cut(s) 313
BsmAI GTCTC 2 cut(s) 548, 738
BsmFI GGGAC 1 cut(s) 710
BsnI GGCC 6 cut(s) 317, 334, 492, 520, 767, 852
Bso31I GGTCTC 1 cut(s) 738
Bsp1286I GDGCHC 1 cut(s) 306
Bsp143I GATC 3 cut(s) 387, 505, 799
BspACI CCGC 1 cut(s) 307
BspANI GGCC 6 cut(s) 317, 334, 492, 520, 767, 852
BspCNI CTCAG 1 cut(s) 472
BspHI TCATGA 1 cut(s) 715
BspLI GGNNCC 1 cut(s) 305
BspPI GGATC 1 cut(s) 513
BspT107I GGYRCC 1 cut(s) 303
BspTNI GGTCTC 1 cut(s) 738
BsrDI GCAATG 2 cut(s) 128, 285
BsrFI RCCGGY 1 cut(s) 313
BsrI ACTGG 2 cut(s) 748, 768
BssAI RCCGGY 1 cut(s) 313
BssECI CCNNGG 1 cut(s) 291
BssMI GATC 3 cut(s) 387, 505, 799
Bst2UI CCWGG 2 cut(s) 185, 267
Bst4CI ACNGT 2 cut(s) 443, 604
Bst6I CTCTTC 3 cut(s) 339, 356, 546
BstAPI GCANNNNNTGC 1 cut(s) 140
BstBAI YACGTR 1 cut(s) 137
BstC8I GCNNGC 3 cut(s) 399, 589, 723
BstDEI CTNAG 1 cut(s) 480
BstF5I GGATG 3 cut(s) 74, 224, 475
BstKTI GATC 3 cut(s) 390, 508, 802
BstMAI GTCTC 2 cut(s) 548, 738
BstMBI GATC 3 cut(s) 387, 505, 799
BstMWI GCNNNNNNNGC 2 cut(s) 17, 140
BstNI CCWGG 2 cut(s) 185, 267
BstNSI RCATGY 3 cut(s) 591, 689, 725
BstSCI CCNGG 4 cut(s) 183, 265, 299, 591
BstSLI GKGCMC 1 cut(s) 306
BstV2I GAAGAC 1 cut(s) 688
BstX2I RGATCY 1 cut(s) 505
BstXI CCANNNNNNTGG 1 cut(s) 755
BstYI RGATCY 1 cut(s) 505
BsuRI GGCC 6 cut(s) 317, 334, 492, 520, 767, 852
BtsCI GGATG 3 cut(s) 74, 224, 475
BtsI GCAGTG 1 cut(s) 715
BtsIMutI CAGTG 1 cut(s) 715
Cac8I GCNNGC 3 cut(s) 399, 589, 723
CciI TCATGA 1 cut(s) 715
Cfr10I RCCGGY 1 cut(s) 313
Cfr13I GGNCC 1 cut(s) 618
CsiI ACCWGGT 1 cut(s) 183
Csp6I GTAC 3 cut(s) 138, 751, 843
CviAII CATG 6 cut(s) 229, 588, 686, 716, 722, 854
CviQI GTAC 3 cut(s) 138, 751, 843
DdeI CTNAG 1 cut(s) 480
DpnI GATC 3 cut(s) 389, 507, 801
DpnII GATC 3 cut(s) 387, 505, 799
EaeI YGGCCR 2 cut(s) 315, 850
Eam1104I CTCTTC 3 cut(s) 339, 356, 546
EarI CTCTTC 3 cut(s) 339, 356, 546
Eco147I AGGCCT 1 cut(s) 520
Eco31I GGTCTC 1 cut(s) 738
Eco47I GGWCC 1 cut(s) 618
EcoRII CCWGG 2 cut(s) 183, 265
EcoT22I ATGCAT 1 cut(s) 128
FaeI CATG 6 cut(s) 232, 591, 689, 719, 725, 857
FaqI GGGAC 1 cut(s) 710
FatI CATG 6 cut(s) 228, 587, 685, 715, 721, 853
FbaI TGATCA 1 cut(s) 799
FblI GTMKAC 1 cut(s) 44
FokI GGATG 3 cut(s) 61, 211, 462
HaeIII GGCC 6 cut(s) 317, 334, 492, 520, 767, 852
HapII CCGG 5 cut(s) 300, 314, 331, 579, 593
Hin1II CATG 6 cut(s) 232, 591, 689, 719, 725, 857
HincII GTYRAC 1 cut(s) 45
HindII GTYRAC 1 cut(s) 45
HinfI GANTC 6 cut(s) 71, 296, 418, 575, 610, 698
HpaII CCGG 5 cut(s) 300, 314, 331, 579, 593
HphI GGTGA 2 cut(s) 440, 593
Hpy166II GTNNAC 1 cut(s) 45
Hpy188I TCNGA 5 cut(s) 295, 324, 574, 609, 615
Hpy188III TCNNGA 4 cut(s) 406, 422, 479, 716
Hpy8I GTNNAC 1 cut(s) 45
HpyAV CCTTC 1 cut(s) 660
HpyCH4III ACNGT 2 cut(s) 443, 604
HpyCH4IV ACGT 1 cut(s) 136
HpyCH4V TGCA 4 cut(s) 95, 126, 352, 725
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 140
HpyF3I CTNAG 1 cut(s) 480
HpySE526I ACGT 1 cut(s) 136
Hsp92II CATG 6 cut(s) 232, 591, 689, 719, 725, 857
Ksp22I TGATCA 1 cut(s) 799
Kzo9I GATC 3 cut(s) 387, 505, 799
LmnI GCTCC 3 cut(s) 8, 268, 758
MabI ACCWGGT 1 cut(s) 183
MaeII ACGT 1 cut(s) 136
MaeIII GTNAC 1 cut(s) 793
MalI GATC 3 cut(s) 389, 507, 801
MboI GATC 3 cut(s) 387, 505, 799
MboII GAAGA 5 cut(s) 78, 356, 373, 563, 693
MflI RGATCY 1 cut(s) 505
MhlI GDGCHC 1 cut(s) 306
MlsI TGGCCA 1 cut(s) 852
MluCI AATT 3 cut(s) 96, 671, 809
MluNI TGGCCA 1 cut(s) 852
MlyI GAGTC 2 cut(s) 427, 692
MmeI TCCRAC 2 cut(s) 484, 552
Mox20I TGGCCA 1 cut(s) 852
Mph1103I ATGCAT 1 cut(s) 128
MscI TGGCCA 1 cut(s) 852
MseI TTAA 3 cut(s) 380, 414, 486
MslI CAYNNNNRTG 2 cut(s) 227, 720
Msp20I TGGCCA 1 cut(s) 852
MspA1I CMGCKG 1 cut(s) 20
MspI CCGG 5 cut(s) 300, 314, 331, 579, 593
MspR9I CCNGG 4 cut(s) 185, 267, 301, 593
MvaI CCWGG 2 cut(s) 185, 267
MwoI GCNNNNNNNGC 2 cut(s) 17, 140
NciI CCSGG 2 cut(s) 301, 593
NdeII GATC 3 cut(s) 387, 505, 799
NlaIII CATG 6 cut(s) 232, 591, 689, 719, 725, 857
NlaIV GGNNCC 1 cut(s) 305
NmuCI GTSAC 1 cut(s) 793
NsiI ATGCAT 1 cut(s) 128
NspI RCATGY 3 cut(s) 591, 689, 725
PaeI GCATGC 2 cut(s) 591, 725
PagI TCATGA 1 cut(s) 715
PceI AGGCCT 1 cut(s) 520
PciI ACATGT 1 cut(s) 685
PfeI GAWTC 4 cut(s) 71, 296, 575, 610
PfoI TCCNGGA 1 cut(s) 265
PleI GAGTC 2 cut(s) 426, 692
PpsI GAGTC 2 cut(s) 426, 692
Ppu21I YACGTR 1 cut(s) 137
PscI ACATGT 1 cut(s) 685
Psp6I CCWGG 2 cut(s) 183, 265
PspGI CCWGG 2 cut(s) 183, 265
PspN4I GGNNCC 1 cut(s) 305
PspPI GGNCC 1 cut(s) 618
PsuI RGATCY 1 cut(s) 505
PvuII CAGCTG 1 cut(s) 20
RsaI GTAC 3 cut(s) 139, 752, 844
RsaNI GTAC 3 cut(s) 138, 751, 843
RseI CAYNNNNRTG 2 cut(s) 227, 720
SalI GTCGAC 1 cut(s) 43
SaqAI TTAA 3 cut(s) 380, 414, 486
Sau3AI GATC 3 cut(s) 387, 505, 799
Sau96I GGNCC 1 cut(s) 618
ScaI AGTACT 1 cut(s) 752
SchI GAGTC 2 cut(s) 427, 692
ScrFI CCNGG 4 cut(s) 185, 267, 301, 593
SduI GDGCHC 1 cut(s) 306
SexAI ACCWGGT 1 cut(s) 183
SinI GGWCC 1 cut(s) 618
SmiMI CAYNNNNRTG 2 cut(s) 227, 720
SphI GCATGC 2 cut(s) 591, 725
Sse9I AATT 3 cut(s) 96, 671, 809
SseBI AGGCCT 1 cut(s) 520
SsiI CCGC 1 cut(s) 307
SspI AATATT 1 cut(s) 61
StuI AGGCCT 1 cut(s) 520
StyD4I CCNGG 4 cut(s) 183, 265, 299, 591
TaaI ACNGT 2 cut(s) 443, 604
TaiI ACGT 1 cut(s) 139
TaqI TCGA 1 cut(s) 44
TasI AATT 3 cut(s) 96, 671, 809
TatI WGTACW 1 cut(s) 750
TfiI GAWTC 4 cut(s) 71, 296, 575, 610
Tru1I TTAA 3 cut(s) 380, 414, 486
Tru9I TTAA 3 cut(s) 380, 414, 486
TscAI CASTG 1 cut(s) 715
TseFI GTSAC 1 cut(s) 793
Tsp45I GTSAC 1 cut(s) 793
TspDTI ATGAA 2 cut(s) 17, 822
TspGWI ACGGA 1 cut(s) 554
TspRI CASTG 1 cut(s) 715
VpaK11BI GGWCC 1 cut(s) 618
XapI RAATTY 1 cut(s) 809
XceI RCATGY 3 cut(s) 591, 689, 725
XmiI GTMKAC 1 cut(s) 44
ZrmI AGTACT 1 cut(s) 752
Zsp2I ATGCAT 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.