MD14G1176000.v1.1

Neprosin activation peptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011754
Physical Location & Seq
Reverse (-)
26925664 .. 26929510
3847 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1176000.v1.1.491

Sequence Viewer

Length: 1503 bp
ATGAAGGGAGTAGGAGAAGAAGACTATAATCCTAATCCACCACAACTCCCATGGACAAAACAGAAGAATGTGAAATCCCACTACACAACAACAAGAAGACACCCAGTCCAATGCAACCAAACACAAACTTCTGATACTGTTTTTGTACCAAACACAAGACAGAGTCAACCTACTCCACCACTCGAACCCAAACATTCCTCTAAACTGGCTCTTCCTACTTACTCTCACACCTCCAACTCCTTCTCATTCGTCTCCATGCTTCTTGTTCTCTTACTTCTTGTTGCTTCTTCTTCTCTCTTTAGTCCTGCATTGTCCCACCCAGTTGTCTCGGATTCTGGCCTACGCCGCCCCTCCCCGGCCAATCAGACTTTTCGGACCGGCTCTATCAACAAGTTGAGCGGGGTCCAAGTCCAATCATATTTAAGGAAGATCAACAAGCCTGCTGTCAAGACTATTCAGAGTCCAGATGGTGATGTGATAGATTGTGTTTTATCTCATCTCCAACCAGCTTTTGATCATCCTGAGCTCAAAGGAAAGAAGCCATTGGAGGAACCAGACAGGCCCAAAAGCTATAACAGCACAGATAAAGTGGGAGAGAGCTATCAGCTATGGGCAGACTCTGGTGAATCATGTCCACAAGGAACAGTTCCGATCCGGCGGACCGGAGAGAAAGACATTTTACGAGCAAGCTCCTTCCAAAGATTCGGAAGAAAGCCGACAAGACATGTGAGGAGAGACTCCTCAGGCAGTGGCCATGAGCATGCGGTTGTGTTTGTTAATGGGGATCAATACTATGGAGCAAAGGCTAGCATCAATGTGTGGGCGCCACGTGTCACTGACCAGTATGAATTTAGCTTATCACAGATTTGGGTCATCTCTGGTTCTTTTGGCAATGATTTAAACACCATTGAAGCTGGATGGCAGGTCAGTCCAGAGTTATATGGAGATAACTATCCAAGATTCTTTACATACTGGACAACAGATGCATATCAAACAACTGGATGCTACAACTTGCTTTGCTCAGGATTTGTCCAAACAAATAACAAGATTGCAATTGGAGCAGCAATCTCCCCAAGGTCCTCTTATGGTAGCAGACAATTTGACATTGGTTTGATGGTTTGGAAGGATCCAAAGCATGGACACTGGTGGCTAGAGTTTGGAAATGGGCTCCTAGTCGGATACTGGCCAGCATTCTTGTTCAGTCACCTGAGGAGCCATGCAAGCATGGTGCAATTTGGGGGTGAAATTGTAAATAGCAGATCTAGAGGATACCACACATCTACACAAATGGGGAGTGGACAATTTGCAGAAGCAGGGTCTGGAAAAGCATCTTATTTCAGAAACTTGCAGGTTGTAGATTGGGATAACAATTTGCTCCCACTTACAAATCTCCACCTCCTGGCTGATCACCCTAATTGTTATGACATAAGACAGGGAAGAAATAATGTGTGGGGAACTTACTTTTACTATGGTGGTCCTGGAAGAAATGTGAGGTGTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

501

Amino Acids

55.96

Weight (kDa)

9.18

Isoelectric Point (pI)

41.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin_AP PF14365 151 - 257 1.3e-36 Neprosin activation peptide
Neprosin PF03080 282 - 493 5e-85 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011754)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50150
fragaria_vesca FvH4_5g05740
malus_domestica MD06G1170400.v1.1 MD14G1176000.v1.1
prunus_persica Prupe.5G170000_v2.0.a1 Prupe.5G170000_v2.0.a1
pyrus_communis pycom06g15180 pycom14g14710
rosa_chinensis RchiOBHm_Chr7g0193071
rosa_laevigata RLG00000004297
rosa_multiflora Rmu_ssc0000002.1_g000010
rosa_roxburghii Rroxscaffold_3G00262400
rosa_rugosa Rorug07G0007400
rosa_samantha Rh7AG132600 Rh7BG133500 Rh7CG136600 Rh7DG134500
rosa_wichuraiana Rw0G015850 Rw7G011330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 913, 1339
AccB1I GGYRCC 1 cut(s) 823
AccB7I CCANNNNNTGG 2 cut(s) 1136, 1399
AccBSI CCGCTC 1 cut(s) 399
AciI CCGC 4 cut(s) 346, 399, 658, 764
AclWI GGATC 4 cut(s) 646, 792, 1121, 1134
AcoI YGGCCR 3 cut(s) 357, 751, 1184
AcsI RAATTY 1 cut(s) 848
AcvI CACGTG 1 cut(s) 830
AcyI GRCGYC 1 cut(s) 824
AfaI GTAC 1 cut(s) 147
AfiI CCNNNNNNNGG 3 cut(s) 355, 1136, 1399
AflIII ACRYGT 2 cut(s) 724, 829
AgsI TTSAA 1 cut(s) 911
AhdI GACNNNNNGTC 1 cut(s) 104
AjnI CCWGG 2 cut(s) 1398, 1477
AluBI AGCT 8 cut(s) 509, 526, 570, 600, 607, 690, 855, 914
AluI AGCT 8 cut(s) 509, 526, 570, 600, 607, 690, 855, 914
Alw21I GWGCWC 1 cut(s) 528
Alw26I GTCTC 3 cut(s) 256, 331, 729
AlwI GGATC 4 cut(s) 646, 792, 1121, 1134
AlwNI CAGNNNCTG 2 cut(s) 620, 1319
AoxI GGCC 5 cut(s) 337, 357, 560, 751, 1184
ApeKI GCWGC 1 cut(s) 1061
ApoI RAATTY 1 cut(s) 848
AspLEI GCGC 1 cut(s) 826
AspS9I GGNCC 6 cut(s) 375, 403, 561, 660, 1077, 1475
AsuC2I CCSGG 1 cut(s) 356
AsuHPI GGTGA 5 cut(s) 482, 635, 1196, 1253, 1400
AsuNHI GCTAGC 1 cut(s) 806
AvaII GGWCC 5 cut(s) 375, 403, 660, 1077, 1475
AxyI CCTNAGG 2 cut(s) 742, 1208
BalI TGGCCA 2 cut(s) 753, 1186
BamHI GGATCC 1 cut(s) 1126
BanI GGYRCC 1 cut(s) 823
BanII GRGCYC 2 cut(s) 528, 1170
BbrPI CACGTG 1 cut(s) 830
BbsI GAAGAC 2 cut(s) 27, 103
Bbv12I GWGCWC 1 cut(s) 528
BbvI GCAGC 1 cut(s) 1073
BccI CCATC 3 cut(s) 461, 912, 1108
BciT130I CCWGG 2 cut(s) 1400, 1479
BciVI GTATCC 2 cut(s) 1172, 1262
BclI TGATCA 2 cut(s) 514, 1405
BcnI CCSGG 1 cut(s) 356
BcoDI GTCTC 3 cut(s) 256, 331, 729
BfaI CTAG 4 cut(s) 807, 1151, 1172, 1263
BfoI RGCGCY 1 cut(s) 827
BfuAI ACCTGC 2 cut(s) 913, 1339
BfuI GTATCC 2 cut(s) 1172, 1262
BglII AGATCT 1 cut(s) 1259
BisI GCNGC 2 cut(s) 346, 1062
BlsI GCNGC 2 cut(s) 347, 1063
Bme1390I CCNGG 3 cut(s) 356, 1400, 1479
Bme18I GGWCC 5 cut(s) 375, 403, 660, 1077, 1475
BmeRI GACNNNNNGTC 1 cut(s) 104
BmgT120I GGNCC 6 cut(s) 375, 403, 561, 660, 1077, 1475
BmiI GGNNCC 6 cut(s) 404, 552, 825, 1128, 1169, 1214
BmrFI CCNGG 3 cut(s) 356, 1400, 1479
BmrI ACTGGG 2 cut(s) 98, 314
BmsI GCATC 4 cut(s) 819, 973, 992, 1337
BmtI GCTAGC 1 cut(s) 810
BmuI ACTGGG 2 cut(s) 98, 314
BpiI GAAGAC 2 cut(s) 27, 103
Bpu10I CCTNAGC 2 cut(s) 522, 1021
BpuMI CCSGG 1 cut(s) 356
BsaAI YACGTR 1 cut(s) 830
BsaBI GATNNNNATC 2 cut(s) 951, 987
BsaHI GRCGYC 1 cut(s) 824
BsaJI CCNNGG 3 cut(s) 50, 354, 1073
BsaWI WCCGGW 1 cut(s) 662
BsaXI ACNNNNNCTCC 2 cut(s) 30, 60
Bsc4I CCNNNNNNNGG 3 cut(s) 355, 1136, 1399
Bse118I RCCGGY 1 cut(s) 377
Bse1I ACTGG 8 cut(s) 104, 210, 320, 841, 977, 1003, 1148, 1187
Bse21I CCTNAGG 2 cut(s) 742, 1208
Bse3DI GCAATG 1 cut(s) 898
Bse8I GATNNNNATC 2 cut(s) 951, 987
BseBI CCWGG 2 cut(s) 1400, 1479
BseDI CCNNGG 3 cut(s) 50, 354, 1073
BseGI GGATG 3 cut(s) 517, 923, 1007
BseJI GATNNNNATC 2 cut(s) 951, 987
BseLI CCNNNNNNNGG 3 cut(s) 355, 1136, 1399
BseMI GCAATG 1 cut(s) 898
BseMII CTCAG 4 cut(s) 513, 756, 1035, 1199
BseNI ACTGG 8 cut(s) 104, 210, 320, 841, 977, 1003, 1148, 1187
BseRI GAGGAG 3 cut(s) 730, 745, 1225
BseXI GCAGC 1 cut(s) 1073
BshFI GGCC 5 cut(s) 339, 359, 562, 753, 1186
BshNI GGYRCC 1 cut(s) 823
BsiHKAI GWGCWC 1 cut(s) 528
BsiSI CCGG 4 cut(s) 356, 378, 655, 663
BslFI GGGAC 1 cut(s) 298
BslI CCNNNNNNNGG 3 cut(s) 355, 1136, 1399
BsmAI GTCTC 3 cut(s) 256, 331, 729
BsmBI CGTCTC 1 cut(s) 256
BsmFI GGGAC 1 cut(s) 298
BsmI GAATGC 1 cut(s) 1190
BsnI GGCC 5 cut(s) 339, 359, 562, 753, 1186
Bsp1286I GDGCHC 2 cut(s) 528, 1170
Bsp143I GATC 7 cut(s) 429, 514, 651, 784, 1126, 1259, 1405
Bsp19I CCATGG 1 cut(s) 50
BspACI CCGC 4 cut(s) 346, 399, 658, 764
BspANI GGCC 5 cut(s) 339, 359, 562, 753, 1186
BspCNI CTCAG 4 cut(s) 514, 755, 1034, 1200
BspLI GGNNCC 6 cut(s) 404, 552, 825, 1128, 1169, 1214
BspMI ACCTGC 2 cut(s) 913, 1339
BspOI GCTAGC 1 cut(s) 810
BspPI GGATC 4 cut(s) 646, 792, 1121, 1134
BspQI GCTCTTC 1 cut(s) 216
BspT107I GGYRCC 1 cut(s) 823
BsrBI CCGCTC 1 cut(s) 399
BsrDI GCAATG 1 cut(s) 898
BsrFI RCCGGY 1 cut(s) 377
BsrI ACTGG 8 cut(s) 104, 210, 320, 841, 977, 1003, 1148, 1187
BssAI RCCGGY 1 cut(s) 377
BssECI CCNNGG 3 cut(s) 50, 354, 1073
BssMI GATC 7 cut(s) 429, 514, 651, 784, 1126, 1259, 1405
BssNI GRCGYC 1 cut(s) 824
BssT1I CCWWGG 2 cut(s) 50, 1073
Bst2UI CCWGG 2 cut(s) 1400, 1479
Bst4CI ACNGT 2 cut(s) 139, 646
Bst6I CTCTTC 1 cut(s) 216
BstACI GRCGYC 1 cut(s) 824
BstBAI YACGTR 1 cut(s) 830
BstC8I GCNNGC 6 cut(s) 441, 688, 762, 808, 1188, 1222
BstDEI CTNAG 4 cut(s) 522, 742, 1021, 1208
BstDSI CCRYGG 1 cut(s) 50
BstF5I GGATG 3 cut(s) 517, 923, 1007
BstH2I RGCGCY 1 cut(s) 827
BstHHI GCGC 1 cut(s) 826
BstKTI GATC 7 cut(s) 432, 517, 654, 787, 1129, 1262, 1408
BstMAI GTCTC 3 cut(s) 256, 331, 729
BstMBI GATC 7 cut(s) 429, 514, 651, 784, 1126, 1259, 1405
BstMWI GCNNNNNNNGC 4 cut(s) 345, 576, 1058, 1221
BstNI CCWGG 2 cut(s) 1400, 1479
BstNSI RCATGY 2 cut(s) 728, 764
BstSCI CCNGG 3 cut(s) 354, 1398, 1477
BstV1I GCAGC 1 cut(s) 1073
BstV2I GAAGAC 2 cut(s) 27, 103
BstX2I RGATCY 2 cut(s) 1126, 1259
BstYI RGATCY 2 cut(s) 1126, 1259
Bsu36I CCTNAGG 2 cut(s) 742, 1208
BsuI GTATCC 2 cut(s) 1172, 1262
BsuRI GGCC 5 cut(s) 339, 359, 562, 753, 1186
BtgI CCRYGG 1 cut(s) 50
BtsCI GGATG 3 cut(s) 517, 923, 1007
BtsI GCAGTG 1 cut(s) 754
BtsIMutI CAGTG 3 cut(s) 754, 834, 1141
BveI ACCTGC 2 cut(s) 913, 1339
Cac8I GCNNGC 6 cut(s) 441, 688, 762, 808, 1188, 1222
CaiI CAGNNNCTG 2 cut(s) 620, 1319
CfoI GCGC 1 cut(s) 826
Cfr10I RCCGGY 1 cut(s) 377
Cfr13I GGNCC 6 cut(s) 375, 403, 561, 660, 1077, 1475
CpoI CGGWCCG 2 cut(s) 375, 660
Csp6I GTAC 1 cut(s) 146
CspI CGGWCCG 2 cut(s) 375, 660
CviQI GTAC 1 cut(s) 146
DdeI CTNAG 4 cut(s) 522, 742, 1021, 1208
DinI GGCGCC 1 cut(s) 825
DpnI GATC 7 cut(s) 431, 516, 653, 786, 1128, 1261, 1407
DpnII GATC 7 cut(s) 429, 514, 651, 784, 1126, 1259, 1405
DraI TTTAAA 1 cut(s) 900
DriI GACNNNNNGTC 1 cut(s) 104
EaeI YGGCCR 3 cut(s) 357, 751, 1184
Eam1104I CTCTTC 1 cut(s) 216
Eam1105I GACNNNNNGTC 1 cut(s) 104
EarI CTCTTC 1 cut(s) 216
EciI GGCGGA 1 cut(s) 673
Ecl136II GAGCTC 1 cut(s) 526
Eco130I CCWWGG 2 cut(s) 50, 1073
Eco24I GRGCYC 2 cut(s) 528, 1170
Eco47I GGWCC 5 cut(s) 375, 403, 660, 1077, 1475
Eco53kI GAGCTC 1 cut(s) 526
Eco72I CACGTG 1 cut(s) 830
Eco81I CCTNAGG 2 cut(s) 742, 1208
EcoICRI GAGCTC 1 cut(s) 526
EcoO109I RGGNCCY 1 cut(s) 1077
EcoRII CCWGG 2 cut(s) 1398, 1477
EcoT14I CCWWGG 2 cut(s) 50, 1073
EcoT22I ATGCAT 1 cut(s) 988
EcoT38I GRGCYC 2 cut(s) 528, 1170
EgeI GGCGCC 1 cut(s) 825
EheI GGCGCC 1 cut(s) 825
ErhI CCWWGG 2 cut(s) 50, 1073
Esp3I CGTCTC 1 cut(s) 256
FalI AAGNNNNNCTT 2 cut(s) 1066, 1098
FaqI GGGAC 1 cut(s) 298
FauI CCCGC 1 cut(s) 392
FbaI TGATCA 2 cut(s) 514, 1405
Fnu4HI GCNGC 2 cut(s) 346, 1062
FokI GGATG 3 cut(s) 504, 930, 1014
FriOI GRGCYC 2 cut(s) 528, 1170
Fsp4HI GCNGC 2 cut(s) 346, 1062
FspBI CTAG 4 cut(s) 807, 1151, 1172, 1263
GlaI GCGC 1 cut(s) 825
GluI GCNGC 2 cut(s) 346, 1062
HaeII RGCGCY 1 cut(s) 827
HaeIII GGCC 5 cut(s) 339, 359, 562, 753, 1186
HapII CCGG 4 cut(s) 356, 378, 655, 663
HhaI GCGC 1 cut(s) 826
Hin1I GRCGYC 1 cut(s) 824
Hin6I GCGC 1 cut(s) 824
HinP1I GCGC 1 cut(s) 824
HincII GTYRAC 1 cut(s) 167
HindII GTYRAC 1 cut(s) 167
HinfI GANTC 8 cut(s) 163, 332, 460, 617, 626, 702, 737, 960
HpaII CCGG 4 cut(s) 356, 378, 655, 663
HphI GGTGA 5 cut(s) 482, 635, 1196, 1253, 1400
Hpy166II GTNNAC 3 cut(s) 167, 635, 1298
Hpy188I TCNGA 9 cut(s) 133, 331, 366, 375, 459, 651, 707, 1178, 1340
Hpy188III TCNNGA 7 cut(s) 448, 464, 521, 932, 1023, 1263, 1320
Hpy8I GTNNAC 3 cut(s) 167, 635, 1298
HpyAV CCTTC 3 cut(s) 250, 703, 1117
HpyCH4III ACNGT 2 cut(s) 139, 646
HpyCH4IV ACGT 1 cut(s) 829
HpyCH4V TGCA 8 cut(s) 114, 308, 986, 1052, 1220, 1231, 1307, 1348
HpyF10VI GCNNNNNNNGC 4 cut(s) 345, 576, 1058, 1221
HpyF3I CTNAG 4 cut(s) 522, 742, 1021, 1208
HpySE526I ACGT 1 cut(s) 829
Hsp92I GRCGYC 1 cut(s) 824
HspAI GCGC 1 cut(s) 824
KasI GGCGCC 1 cut(s) 823
Ksp22I TGATCA 2 cut(s) 514, 1405
Kzo9I GATC 7 cut(s) 429, 514, 651, 784, 1126, 1259, 1405
LguI GCTCTTC 1 cut(s) 216
LmnI GCTCC 6 cut(s) 695, 797, 1058, 1173, 1212, 1380
Lsp1109I GCAGC 1 cut(s) 1073
LweI GCATC 4 cut(s) 819, 973, 992, 1337
MaeI CTAG 4 cut(s) 807, 1151, 1172, 1263
MaeII ACGT 1 cut(s) 829
MaeIII GTNAC 2 cut(s) 832, 1202
MalI GATC 7 cut(s) 431, 516, 653, 786, 1128, 1261, 1407
MbiI CCGCTC 1 cut(s) 399
MboI GATC 7 cut(s) 429, 514, 651, 784, 1126, 1259, 1405
MfeI CAATTG 1 cut(s) 1053
MflI RGATCY 2 cut(s) 1126, 1259
MhlI GDGCHC 2 cut(s) 528, 1170
MlsI TGGCCA 2 cut(s) 753, 1186
MluCI AATT 8 cut(s) 848, 1053, 1097, 1232, 1245, 1301, 1369, 1414
MluNI TGGCCA 2 cut(s) 753, 1186
Mly113I GGCGCC 1 cut(s) 824
MlyI GAGTC 4 cut(s) 172, 469, 611, 731
MmeI TCCRAC 3 cut(s) 258, 526, 1156
Mox20I TGGCCA 2 cut(s) 753, 1186
Mph1103I ATGCAT 1 cut(s) 988
MscI TGGCCA 2 cut(s) 753, 1186
MseI TTAA 3 cut(s) 422, 777, 899
MslI CAYNNNNRTG 2 cut(s) 759, 815
Msp20I TGGCCA 2 cut(s) 753, 1186
MspI CCGG 4 cut(s) 356, 378, 655, 663
MspR9I CCNGG 3 cut(s) 356, 1400, 1479
MunI CAATTG 1 cut(s) 1053
Mva1269I GAATGC 1 cut(s) 1190
MvaI CCWGG 2 cut(s) 1400, 1479
MwoI GCNNNNNNNGC 4 cut(s) 345, 576, 1058, 1221
NarI GGCGCC 1 cut(s) 824
NciI CCSGG 1 cut(s) 356
NcoI CCATGG 1 cut(s) 50
NdeII GATC 7 cut(s) 429, 514, 651, 784, 1126, 1259, 1405
NheI GCTAGC 1 cut(s) 806
NlaIV GGNNCC 6 cut(s) 404, 552, 825, 1128, 1169, 1214
NmuCI GTSAC 2 cut(s) 832, 1202
NsiI ATGCAT 1 cut(s) 988
NspI RCATGY 2 cut(s) 728, 764
PaeI GCATGC 1 cut(s) 764
PciI ACATGT 1 cut(s) 724
PciSI GCTCTTC 1 cut(s) 216
PctI GAATGC 1 cut(s) 1190
PfeI GAWTC 4 cut(s) 332, 626, 702, 960
PflFI GACNNNGTC 1 cut(s) 162
PflMI CCANNNNNTGG 2 cut(s) 1136, 1399
PfoI TCCNGGA 1 cut(s) 1477
PkrI GCNGC 2 cut(s) 347, 1063
PleI GAGTC 4 cut(s) 171, 468, 611, 731
PluTI GGCGCC 1 cut(s) 827
PmaCI CACGTG 1 cut(s) 830
PmlI CACGTG 1 cut(s) 830
PpsI GAGTC 4 cut(s) 171, 468, 611, 731
Ppu21I YACGTR 1 cut(s) 830
PpuMI RGGWCCY 1 cut(s) 1077
PscI ACATGT 1 cut(s) 724
Psp124BI GAGCTC 1 cut(s) 528
Psp5II RGGWCCY 1 cut(s) 1077
Psp6I CCWGG 2 cut(s) 1398, 1477
PspCI CACGTG 1 cut(s) 830
PspGI CCWGG 2 cut(s) 1398, 1477
PspN4I GGNNCC 6 cut(s) 404, 552, 825, 1128, 1169, 1214
PspPI GGNCC 6 cut(s) 375, 403, 561, 660, 1077, 1475
PspPPI RGGWCCY 1 cut(s) 1077
PstNI CAGNNNCTG 2 cut(s) 620, 1319
PsuI RGATCY 2 cut(s) 1126, 1259
PsyI GACNNNGTC 1 cut(s) 162
RsaI GTAC 1 cut(s) 147
RsaNI GTAC 1 cut(s) 146
RseI CAYNNNNRTG 2 cut(s) 759, 815
Rsr2I CGGWCCG 2 cut(s) 375, 660
RsrII CGGWCCG 2 cut(s) 375, 660
SacI GAGCTC 1 cut(s) 528
SapI GCTCTTC 1 cut(s) 216
SaqAI TTAA 3 cut(s) 422, 777, 899
SatI GCNGC 2 cut(s) 346, 1062
Sau3AI GATC 7 cut(s) 429, 514, 651, 784, 1126, 1259, 1405
Sau96I GGNCC 6 cut(s) 375, 403, 561, 660, 1077, 1475
SchI GAGTC 4 cut(s) 172, 469, 611, 731
ScrFI CCNGG 3 cut(s) 356, 1400, 1479
SduI GDGCHC 2 cut(s) 528, 1170
SfaNI GCATC 4 cut(s) 819, 973, 992, 1337
SfoI GGCGCC 1 cut(s) 825
SinI GGWCC 5 cut(s) 375, 403, 660, 1077, 1475
SmiMI CAYNNNNRTG 2 cut(s) 759, 815
SphI GCATGC 1 cut(s) 764
Sse9I AATT 8 cut(s) 848, 1053, 1097, 1232, 1245, 1301, 1369, 1414
SsiI CCGC 4 cut(s) 346, 399, 658, 764
SspDI GGCGCC 1 cut(s) 823
SspMI CTAG 4 cut(s) 807, 1151, 1172, 1263
SstI GAGCTC 1 cut(s) 528
StyD4I CCNGG 3 cut(s) 354, 1398, 1477
StyI CCWWGG 2 cut(s) 50, 1073
TaaI ACNGT 2 cut(s) 139, 646
TaiI ACGT 1 cut(s) 832
TaqI TCGA 1 cut(s) 183
TasI AATT 8 cut(s) 848, 1053, 1097, 1232, 1245, 1301, 1369, 1414
TauI GCSGC 1 cut(s) 348
TfiI GAWTC 4 cut(s) 332, 626, 702, 960
Tru1I TTAA 3 cut(s) 422, 777, 899
Tru9I TTAA 3 cut(s) 422, 777, 899
TscAI CASTG 3 cut(s) 754, 841, 1148
TseFI GTSAC 2 cut(s) 832, 1202
TseI GCWGC 1 cut(s) 1061
Tsp45I GTSAC 2 cut(s) 832, 1202
TspDTI ATGAA 2 cut(s) 17, 861
TspRI CASTG 3 cut(s) 754, 841, 1148
Tth111I GACNNNGTC 1 cut(s) 162
Van91I CCANNNNNTGG 2 cut(s) 1136, 1399
VpaK11BI GGWCC 5 cut(s) 375, 403, 660, 1077, 1475
XapI RAATTY 1 cut(s) 848
XbaI TCTAGA 1 cut(s) 1262
XceI RCATGY 2 cut(s) 728, 764
XcmI CCANNNNNNNNNTGG 1 cut(s) 48
XspI CTAG 4 cut(s) 807, 1151, 1172, 1263
Zsp2I ATGCAT 1 cut(s) 988
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.