RLG00000004297

Neprosin activation peptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0011754
Physical Location & Seq
Forward (+)
58076245 .. 58079143
2899 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004297

Sequence Viewer

Length: 1467 bp
ATGAAAGGAGCTTATTCAGTCGTTGTTCTTGTTGGTTCTGCTCTGTCGACAAAAGAGAGGAATATTGAAGCAGAATCATCCTCATCATCTTCTTGTGCAATTCAGCACACAACAGGACAAGAGCAATGCATACAAGCACGTACTGCTGAAACATTACAAACAACTCCACTCAAACATTCCTCTAACCTGGTTGCTCATACTTTCCACACCACCCCTTTCTCATTCACATCCATGCTTCTTGTTCTCTTACTTCTTGTTGCTTTTGCTCCTGGACTTTCCTCCCTCCCCATTGCCTCGGATTCCGGGCACCGCCCACCTGCCAATCAGACATTCCGGCCTGGTGGGGAAGAGATGCAGAACTTGAAGAGGGTCAGCTCTTATTTAAGCAAGATCAACAAGCCTGCTGTCAAGACTATTAAGAGTCCTGATGGTGATGTGATAGACTGCGTTTTAGCCCATCTCCAACCAGCTTTTGACCATCCTGAGCTTAAAGGCCAAAAACCATTGGATCCACCAGAGAGGCCTAAATCCCATAACAGCACGGATATAGTGGAAGAGACTTATCAGCTATGGTCTGAATCCGGTGAGGCATGCCCGGAGGGGACTGTTCCGATTCGGAGGACCACAGACGAAGACATTTTGAGAGCAAGTTCAGTTCAAAGATTTGGAAGGAAATTACCAAGAAGACATGTGAGGAGGGACTCATCAGGCAGTGGTCATGAGCATGCAGTTGTCTTTGTAAATGGAGACCAGTACTATGGAGCAAAGGCCAGTATAAATGTGTGGTCGCCAAGAGTGACTGATCAATATGAATTTAGCTTATCACAGATTTGGGTCATCTCTGGTACATTTGGCCATGACCTAAATACCATTGAAGCTGGTTGGCAGGTTAGCCCAGAGTTATATGGAGACAACTACCCAAGATTCTTTACATATTGGACAACAGATGCATATCAAGCTACTGGATGCTACAACTTGCTGTGCTCTGGCTTTGTCCAAACCAATAATAAGATTGCTATTGGAGCTGCTATTTCTCCAAGGTCTTCCTTCAATGGCAGACAATTTGATATTGGCTTAATGGTTTGGAAGGATCCAAAGCATGGGCACTGGTGGCTAGAATTTGGTAATGGCCTACTAGTTGGGTATTGGCCAGCCTTCTTGTTTAGTCACTTGAGAAGTCATGCCAGCATGGTACAATTTGGAGGTGAAATTGTAAATAGTAGATCAAGGGGGTACCACACATCTACACAAATGGGCAGTGGCCAATTTGCAGGAGCAGGTTTTGGAAAGGCATCTTATTTCAGAAATTTGCAAGTAGTGGATTGGGATAATAATCTTCTTCCTCTTAATAACCTTCACCTCACTGCTGATCACCCCAATTGTTATGATATAAGACAAGGAAGAAATAATGCATGGGGGACATACTTTTACTATGGAGGTCCTGGAAGAAATGTAAGGTGTCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

489

Amino Acids

54.18

Weight (kDa)

8.07

Isoelectric Point (pI)

42.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin_AP PF14365 138 - 245 1.5e-38 Neprosin activation peptide
Neprosin PF03080 270 - 481 3e-84 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011754)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50150
fragaria_vesca FvH4_5g05740
malus_domestica MD06G1170400.v1.1 MD14G1176000.v1.1
prunus_persica Prupe.5G170000_v2.0.a1 Prupe.5G170000_v2.0.a1
pyrus_communis pycom06g15180 pycom14g14710
rosa_chinensis RchiOBHm_Chr7g0193071
rosa_laevigata RLG00000004297
rosa_multiflora Rmu_ssc0000002.1_g000010
rosa_roxburghii Rroxscaffold_3G00262400
rosa_rugosa Rorug07G0007400
rosa_samantha Rh7AG132600 Rh7BG133500 Rh7CG136600 Rh7DG134500
rosa_wichuraiana Rw0G015850 Rw7G011330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 325
Acc36I ACCTGC 3 cut(s) 325, 877, 1268
Acc65I GGTACC 1 cut(s) 1233
AccB1I GGYRCC 2 cut(s) 306, 1233
AccB7I CCANNNNNTGG 1 cut(s) 1100
AccI GTMKAC 1 cut(s) 47
AciI CCGC 1 cut(s) 310
AclWI GGATC 4 cut(s) 503, 516, 1085, 1098
AcoI YGGCCR 3 cut(s) 853, 1148, 1261
AcsI RAATTY 3 cut(s) 812, 1118, 1306
AfaI GTAC 5 cut(s) 142, 755, 847, 1194, 1235
AfiI CCNNNNNNNGG 1 cut(s) 1100
AflIII ACRYGT 1 cut(s) 688
AgsI TTSAA 5 cut(s) 68, 364, 659, 875, 1051
AhlI ACTAGT 1 cut(s) 1135
AjnI CCWGG 4 cut(s) 186, 268, 337, 1441
AjuI GAANNNNNNNTTGG 2 cut(s) 314, 346
AluBI AGCT 9 cut(s) 11, 375, 470, 487, 568, 819, 878, 959, 1025
AluI AGCT 9 cut(s) 11, 375, 470, 487, 568, 819, 878, 959, 1025
Alw21I GWGCWC 1 cut(s) 986
Alw26I GTCTC 3 cut(s) 551, 741, 903
AlwI GGATC 4 cut(s) 503, 516, 1085, 1098
AoxI GGCC 8 cut(s) 335, 493, 521, 768, 853, 1129, 1148, 1261
ApeKI GCWGC 1 cut(s) 1025
ApoI RAATTY 3 cut(s) 812, 1118, 1306
Asp718I GGTACC 1 cut(s) 1233
AspS9I GGNCC 2 cut(s) 621, 1439
AsuC2I CCSGG 2 cut(s) 304, 596
AsuHPI GGTGA 5 cut(s) 443, 596, 1217, 1349, 1364
AvaII GGWCC 2 cut(s) 621, 1439
BaeGI GKGCMC 2 cut(s) 309, 1107
BalI TGGCCA 3 cut(s) 855, 1150, 1263
BamHI GGATCC 2 cut(s) 508, 1090
BanI GGYRCC 2 cut(s) 306, 1233
BbsI GAAGAC 3 cut(s) 639, 691, 1035
Bbv12I GWGCWC 1 cut(s) 986
BbvI GCAGC 1 cut(s) 1012
BccI CCATC 3 cut(s) 422, 465, 486
BciT130I CCWGG 4 cut(s) 188, 270, 339, 1443
BclI TGATCA 2 cut(s) 802, 1369
BcnI CCSGG 2 cut(s) 304, 596
BcoDI GTCTC 3 cut(s) 551, 741, 903
BcuI ACTAGT 1 cut(s) 1135
BfaI CTAG 2 cut(s) 1115, 1136
BfuAI ACCTGC 3 cut(s) 325, 877, 1268
BisI GCNGC 1 cut(s) 1026
BlsI GCNGC 1 cut(s) 1027
BmcAI AGTACT 1 cut(s) 755
Bme1390I CCNGG 6 cut(s) 188, 270, 304, 339, 596, 1443
Bme18I GGWCC 2 cut(s) 621, 1439
BmgT120I GGNCC 2 cut(s) 621, 1439
BmiI GGNNCC 4 cut(s) 308, 510, 1092, 1235
BmrFI CCNGG 6 cut(s) 188, 270, 304, 339, 596, 1443
BmsI GCATC 4 cut(s) 342, 937, 956, 1301
BpiI GAAGAC 3 cut(s) 639, 691, 1035
Bpu10I CCTNAGC 1 cut(s) 483
BpuEI CTTGAG 1 cut(s) 1192
BpuMI CCSGG 2 cut(s) 304, 596
BsaAI YACGTR 1 cut(s) 140
BsaBI GATNNNNATC 2 cut(s) 951, 1332
BsaI GGTCTC 1 cut(s) 741
BsaJI CCNNGG 2 cut(s) 294, 1037
BsaWI WCCGGW 1 cut(s) 581
Bsc4I CCNNNNNNNGG 1 cut(s) 1100
Bse1I ACTGG 4 cut(s) 751, 771, 967, 1112
Bse3DI GCAATG 2 cut(s) 131, 288
Bse8I GATNNNNATC 2 cut(s) 951, 1332
BseBI CCWGG 4 cut(s) 188, 270, 339, 1443
BseDI CCNNGG 2 cut(s) 294, 1037
BseGI GGATG 4 cut(s) 77, 227, 478, 971
BseJI GATNNNNATC 2 cut(s) 951, 1332
BseLI CCNNNNNNNGG 1 cut(s) 1100
BseMI GCAATG 2 cut(s) 131, 288
BseMII CTCAG 1 cut(s) 474
BseNI ACTGG 4 cut(s) 751, 771, 967, 1112
BseRI GAGGAG 1 cut(s) 709
BseSI GKGCMC 2 cut(s) 309, 1107
BseXI GCAGC 1 cut(s) 1012
BshFI GGCC 8 cut(s) 337, 495, 523, 770, 855, 1131, 1150, 1263
BshNI GGYRCC 2 cut(s) 306, 1233
BsiHKAI GWGCWC 1 cut(s) 986
BsiSI CCGG 4 cut(s) 303, 334, 582, 596
BslFI GGGAC 3 cut(s) 616, 713, 1432
BslI CCNNNNNNNGG 1 cut(s) 1100
BsmAI GTCTC 3 cut(s) 551, 741, 903
BsmFI GGGAC 3 cut(s) 616, 713, 1432
BsnI GGCC 8 cut(s) 337, 495, 523, 770, 855, 1131, 1150, 1263
Bso31I GGTCTC 1 cut(s) 741
Bsp1286I GDGCHC 3 cut(s) 309, 986, 1107
Bsp143I GATC 6 cut(s) 390, 508, 802, 1090, 1223, 1369
BspACI CCGC 1 cut(s) 310
BspANI GGCC 8 cut(s) 337, 495, 523, 770, 855, 1131, 1150, 1263
BspCNI CTCAG 1 cut(s) 475
BspHI TCATGA 1 cut(s) 718
BspLI GGNNCC 4 cut(s) 308, 510, 1092, 1235
BspMI ACCTGC 3 cut(s) 325, 877, 1268
BspPI GGATC 4 cut(s) 503, 516, 1085, 1098
BspT107I GGYRCC 2 cut(s) 306, 1233
BspTNI GGTCTC 1 cut(s) 741
BsrDI GCAATG 2 cut(s) 131, 288
BsrI ACTGG 4 cut(s) 751, 771, 967, 1112
BssECI CCNNGG 2 cut(s) 294, 1037
BssMI GATC 6 cut(s) 390, 508, 802, 1090, 1223, 1369
BssT1I CCWWGG 1 cut(s) 1037
Bst2UI CCWGG 4 cut(s) 188, 270, 339, 1443
Bst4CI ACNGT 1 cut(s) 607
Bst6I CTCTTC 3 cut(s) 342, 359, 549
BstAPI GCANNNNNTGC 1 cut(s) 143
BstBAI YACGTR 1 cut(s) 140
BstC8I GCNNGC 5 cut(s) 402, 592, 726, 1152, 1186
BstDEI CTNAG 1 cut(s) 483
BstF5I GGATG 4 cut(s) 77, 227, 478, 971
BstKTI GATC 6 cut(s) 393, 511, 805, 1093, 1226, 1372
BstMAI GTCTC 3 cut(s) 551, 741, 903
BstMBI GATC 6 cut(s) 390, 508, 802, 1090, 1223, 1369
BstMWI GCNNNNNNNGC 4 cut(s) 143, 956, 1022, 1111
BstNI CCWGG 4 cut(s) 188, 270, 339, 1443
BstNSI RCATGY 3 cut(s) 594, 692, 728
BstSCI CCNGG 6 cut(s) 186, 268, 302, 337, 594, 1441
BstSLI GKGCMC 2 cut(s) 309, 1107
BstV1I GCAGC 1 cut(s) 1012
BstV2I GAAGAC 3 cut(s) 639, 691, 1035
BstX2I RGATCY 2 cut(s) 508, 1090
BstXI CCANNNNNNTGG 1 cut(s) 758
BstYI RGATCY 2 cut(s) 508, 1090
BsuRI GGCC 8 cut(s) 337, 495, 523, 770, 855, 1131, 1150, 1263
BtsCI GGATG 4 cut(s) 77, 227, 478, 971
BtsI GCAGTG 3 cut(s) 718, 1264, 1362
BtsIMutI CAGTG 4 cut(s) 718, 1105, 1264, 1362
BveI ACCTGC 3 cut(s) 325, 877, 1268
Cac8I GCNNGC 5 cut(s) 402, 592, 726, 1152, 1186
CciI TCATGA 1 cut(s) 718
Cfr13I GGNCC 2 cut(s) 621, 1439
CsiI ACCWGGT 1 cut(s) 186
Csp6I GTAC 5 cut(s) 141, 754, 846, 1193, 1234
CviQI GTAC 5 cut(s) 141, 754, 846, 1193, 1234
DdeI CTNAG 1 cut(s) 483
DpnI GATC 6 cut(s) 392, 510, 804, 1092, 1225, 1371
DpnII GATC 6 cut(s) 390, 508, 802, 1090, 1223, 1369
EaeI YGGCCR 3 cut(s) 853, 1148, 1261
Eam1104I CTCTTC 3 cut(s) 342, 359, 549
EarI CTCTTC 3 cut(s) 342, 359, 549
Eco130I CCWWGG 1 cut(s) 1037
Eco147I AGGCCT 1 cut(s) 523
Eco31I GGTCTC 1 cut(s) 741
Eco47I GGWCC 2 cut(s) 621, 1439
EcoO109I RGGNCCY 1 cut(s) 1439
EcoRII CCWGG 4 cut(s) 186, 268, 337, 1441
EcoT14I CCWWGG 1 cut(s) 1037
EcoT22I ATGCAT 3 cut(s) 131, 952, 1414
ErhI CCWWGG 1 cut(s) 1037
FalI AAGNNNNNCTT 1 cut(s) 1447
FaqI GGGAC 3 cut(s) 616, 713, 1432
FbaI TGATCA 2 cut(s) 802, 1369
FblI GTMKAC 1 cut(s) 47
Fnu4HI GCNGC 1 cut(s) 1026
FokI GGATG 4 cut(s) 64, 214, 465, 978
Fsp4HI GCNGC 1 cut(s) 1026
FspBI CTAG 2 cut(s) 1115, 1136
GluI GCNGC 1 cut(s) 1026
HaeIII GGCC 8 cut(s) 337, 495, 523, 770, 855, 1131, 1150, 1263
HapII CCGG 4 cut(s) 303, 334, 582, 596
HincII GTYRAC 1 cut(s) 48
HindII GTYRAC 1 cut(s) 48
HinfI GANTC 7 cut(s) 74, 299, 421, 578, 613, 701, 924
HpaII CCGG 4 cut(s) 303, 334, 582, 596
HphI GGTGA 5 cut(s) 443, 596, 1217, 1349, 1364
Hpy166II GTNNAC 1 cut(s) 48
Hpy188I TCNGA 6 cut(s) 298, 327, 577, 612, 618, 1304
Hpy188III TCNNGA 4 cut(s) 409, 425, 482, 719
Hpy8I GTNNAC 1 cut(s) 48
HpyAV CCTTC 5 cut(s) 663, 1057, 1081, 1165, 1364
HpyCH4III ACNGT 1 cut(s) 607
HpyCH4IV ACGT 1 cut(s) 139
HpyCH4V TGCA 8 cut(s) 98, 129, 355, 728, 950, 1271, 1312, 1412
HpyF10VI GCNNNNNNNGC 4 cut(s) 143, 956, 1022, 1111
HpyF3I CTNAG 1 cut(s) 483
HpySE526I ACGT 1 cut(s) 139
KpnI GGTACC 1 cut(s) 1237
Ksp22I TGATCA 2 cut(s) 802, 1369
Kzo9I GATC 6 cut(s) 390, 508, 802, 1090, 1223, 1369
LmnI GCTCC 5 cut(s) 8, 271, 761, 1022, 1274
Lsp1109I GCAGC 1 cut(s) 1012
LweI GCATC 4 cut(s) 342, 937, 956, 1301
MabI ACCWGGT 1 cut(s) 186
MaeI CTAG 2 cut(s) 1115, 1136
MaeII ACGT 1 cut(s) 139
MaeIII GTNAC 2 cut(s) 796, 1166
MalI GATC 6 cut(s) 392, 510, 804, 1092, 1225, 1371
MboI GATC 6 cut(s) 390, 508, 802, 1090, 1223, 1369
MfeI CAATTG 1 cut(s) 1378
MflI RGATCY 2 cut(s) 508, 1090
MhlI GDGCHC 3 cut(s) 309, 986, 1107
MlsI TGGCCA 3 cut(s) 855, 1150, 1263
MluNI TGGCCA 3 cut(s) 855, 1150, 1263
MlyI GAGTC 2 cut(s) 430, 695
MmeI TCCRAC 1 cut(s) 487
Mox20I TGGCCA 3 cut(s) 855, 1150, 1263
Mph1103I ATGCAT 3 cut(s) 131, 952, 1414
MscI TGGCCA 3 cut(s) 855, 1150, 1263
MseI TTAA 5 cut(s) 383, 417, 489, 1076, 1347
MslI CAYNNNNRTG 2 cut(s) 230, 723
Msp20I TGGCCA 3 cut(s) 855, 1150, 1263
MspI CCGG 4 cut(s) 303, 334, 582, 596
MspR9I CCNGG 6 cut(s) 188, 270, 304, 339, 596, 1443
MunI CAATTG 1 cut(s) 1378
MvaI CCWGG 4 cut(s) 188, 270, 339, 1443
MwoI GCNNNNNNNGC 4 cut(s) 143, 956, 1022, 1111
NciI CCSGG 2 cut(s) 304, 596
NdeII GATC 6 cut(s) 390, 508, 802, 1090, 1223, 1369
NlaIV GGNNCC 4 cut(s) 308, 510, 1092, 1235
NmuCI GTSAC 2 cut(s) 796, 1166
NsiI ATGCAT 3 cut(s) 131, 952, 1414
NspI RCATGY 3 cut(s) 594, 692, 728
PaeI GCATGC 2 cut(s) 594, 728
PagI TCATGA 1 cut(s) 718
PaqCI CACCTGC 1 cut(s) 325
PceI AGGCCT 1 cut(s) 523
PciI ACATGT 1 cut(s) 688
PfeI GAWTC 5 cut(s) 74, 299, 578, 613, 924
PflMI CCANNNNNTGG 1 cut(s) 1100
PfoI TCCNGGA 2 cut(s) 268, 1441
PkrI GCNGC 1 cut(s) 1027
PleI GAGTC 2 cut(s) 429, 695
PpsI GAGTC 2 cut(s) 429, 695
Ppu21I YACGTR 1 cut(s) 140
PpuMI RGGWCCY 1 cut(s) 1439
PscI ACATGT 1 cut(s) 688
Psp5II RGGWCCY 1 cut(s) 1439
Psp6I CCWGG 4 cut(s) 186, 268, 337, 1441
PspGI CCWGG 4 cut(s) 186, 268, 337, 1441
PspN4I GGNNCC 4 cut(s) 308, 510, 1092, 1235
PspPI GGNCC 2 cut(s) 621, 1439
PspPPI RGGWCCY 1 cut(s) 1439
PsuI RGATCY 2 cut(s) 508, 1090
RsaI GTAC 5 cut(s) 142, 755, 847, 1194, 1235
RsaNI GTAC 5 cut(s) 141, 754, 846, 1193, 1234
RseI CAYNNNNRTG 2 cut(s) 230, 723
SalI GTCGAC 1 cut(s) 46
SaqAI TTAA 5 cut(s) 383, 417, 489, 1076, 1347
SatI GCNGC 1 cut(s) 1026
Sau3AI GATC 6 cut(s) 390, 508, 802, 1090, 1223, 1369
Sau96I GGNCC 2 cut(s) 621, 1439
ScaI AGTACT 1 cut(s) 755
SchI GAGTC 2 cut(s) 430, 695
ScrFI CCNGG 6 cut(s) 188, 270, 304, 339, 596, 1443
SduI GDGCHC 3 cut(s) 309, 986, 1107
SexAI ACCWGGT 1 cut(s) 186
SfaNI GCATC 4 cut(s) 342, 937, 956, 1301
SinI GGWCC 2 cut(s) 621, 1439
SmiMI CAYNNNNRTG 2 cut(s) 230, 723
SmlI CTYRAG 1 cut(s) 1171
SmoI CTYRAG 1 cut(s) 1171
SpeI ACTAGT 1 cut(s) 1135
SphI GCATGC 2 cut(s) 594, 728
SseBI AGGCCT 1 cut(s) 523
SsiI CCGC 1 cut(s) 310
SspI AATATT 1 cut(s) 64
SspMI CTAG 2 cut(s) 1115, 1136
StuI AGGCCT 1 cut(s) 523
StyD4I CCNGG 6 cut(s) 186, 268, 302, 337, 594, 1441
StyI CCWWGG 1 cut(s) 1037
TaaI ACNGT 1 cut(s) 607
TaiI ACGT 1 cut(s) 142
TaqI TCGA 1 cut(s) 47
TatI WGTACW 1 cut(s) 753
TfiI GAWTC 5 cut(s) 74, 299, 578, 613, 924
Tru1I TTAA 5 cut(s) 383, 417, 489, 1076, 1347
Tru9I TTAA 5 cut(s) 383, 417, 489, 1076, 1347
TscAI CASTG 4 cut(s) 718, 1112, 1264, 1369
TseFI GTSAC 2 cut(s) 796, 1166
TseI GCWGC 1 cut(s) 1025
Tsp45I GTSAC 2 cut(s) 796, 1166
TspDTI ATGAA 2 cut(s) 17, 825
TspGWI ACGGA 1 cut(s) 557
TspRI CASTG 4 cut(s) 718, 1112, 1264, 1369
Van91I CCANNNNNTGG 1 cut(s) 1100
VpaK11BI GGWCC 2 cut(s) 621, 1439
XapI RAATTY 3 cut(s) 812, 1118, 1306
XceI RCATGY 3 cut(s) 594, 692, 728
XmiI GTMKAC 1 cut(s) 47
XspI CTAG 2 cut(s) 1115, 1136
ZrmI AGTACT 1 cut(s) 755
Zsp2I ATGCAT 3 cut(s) 131, 952, 1414
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.