Prupe.5G170000_v2.0.a1

Neprosin activation peptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0011754
Physical Location & Seq
Reverse (-)
14573525 .. 14576587
3063 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G170000.1

Sequence Viewer

Length: 1473 bp
ATGAAGGGAGATTATTCAGGTCCATGGACAAAACAGAAGAATGAAAACAACACACCCTCCTCATGTTGTTATGCAATTCAGCACACAACAAGAAGACAACAACAATGCATACAAACACAAACTGTTGTTGTTGTTGTACCAAAACAGAAACCTACTCCACTAAAACATTCCTCTAAACTGGCTCTTCCTCCTTGCCACATCCACACCAACACTTTCTCATTCGTTCCCATGCTTCTTGTTCTTTTATTTCTTGTTGCTTCTTCTCTCTTCACCCCTGCATTGTCACACCCAATTGTCTCAGATTCCGGCCGCCGCCTCTCGCCGGCCAATCAGACTTTTCGGCCAGGTTCAGAGTTGAACAAGTTGAGGGTTGTCCAATCATATTTAAAGAAGATCAACAAGCCTGCAGTCAAGACTATCCAGAGCCCTGATGGTGATGTGATAGACTGTGTTTTATCTCATCTTCAACCAGCTTTTGACCATCCTGAGCTTAAAGGACAGAAGCCATTGGATGAACCAGAGAGGCCCAAAGACAAAGGCCGTAACAACACAGATATAGTGGGAGACAGTTATCAACTGTGGTCAGATTCTGGTGAATCCTGCCCACAAGGAACTGTTCCAATCAGGAGGACAGCAGATAAAGACATTTTAAGAGCAAGCTCCATCAGAAGATTTGGAAGAAAACCAAGAAGAAGTGTGAGGAGAGACTCATCAGGCAGTGGCCATGAGCATGCAGTTGTGTTTGTAAATGGAGATCAATACTATGGAGCAAAGGCCAGCATAAATGTGTGGGCTCCACGTGTGACTGATCAATATGAATTTAGCTTGTCACAGATTTGGGTAATCTCTGGTTCATTTGGCCATGATCTAAATACCATTGAAGCTGGATGGCAGGTTAGCCCAGAGTTATATGGAGACAACTACCCTAGATTCTTTACATACTGGACAACAGATGCATATCAAGCCACTGGATGCTACAACTTGCTTTGCTCTGGCTTTGTCCAAACTAATAACAAGATTGCTATTGGAGCAGCAATATCTCCAAGGTCTTCTTATGGTAGCAGACAATTTGACATTGGCTTGATGGTTTGGAAGGATCCAAAGCACGGTCACTGGTGGCTAGAATTTGGTAATGGCCTACTAGTTGGATATTGGCCTGCATTCTTGTTTAGTCACCTGAGGAGCCATGCAAGCATGGTGCAATTTGGAGGAGAAATTGTAAATAGTAGATCAATGGGATACCACACATCTACACAAATGGGGAGTGGCCAATTTGCAGAAGCTGGATTTGGAAAAGCATCTTACTTCAGAAATTTGCAAGTAGTTGATTGGGATAATAACCTACTTCCTCTCACAAATCTTCACCTCCTGGCTGATCATCCAAATTGTTATGACATAAGACAAGGAAGAAACAATGTATGGGGGACTTACTTTTACTATGGAGGCCCTGGAAGAAATGTAAGGTGTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

491

Amino Acids

55.02

Weight (kDa)

9.25

Isoelectric Point (pI)

43.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011754)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50150
fragaria_vesca FvH4_5g05740
malus_domestica MD06G1170400.v1.1 MD14G1176000.v1.1
prunus_persica Prupe.5G170000_v2.0.a1 Prupe.5G170000_v2.0.a1
pyrus_communis pycom06g15180 pycom14g14710
rosa_chinensis RchiOBHm_Chr7g0193071
rosa_laevigata RLG00000004297
rosa_multiflora Rmu_ssc0000002.1_g000010
rosa_roxburghii Rroxscaffold_3G00262400
rosa_rugosa Rorug07G0007400
rosa_samantha Rh7AG132600 Rh7BG133500 Rh7CG136600 Rh7DG134500
rosa_wichuraiana Rw0G015850 Rw7G011330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 883
AciI CCGC 2 cut(s) 310, 313
AclWI GGATC 2 cut(s) 1091, 1104
AcoI YGGCCR 6 cut(s) 307, 324, 341, 721, 859, 1267
AcsI RAATTY 3 cut(s) 818, 1124, 1312
AcuI CTGAAG 1 cut(s) 1291
AcvI CACGTG 1 cut(s) 800
AfaI GTAC 1 cut(s) 138
AfiI CCNNNNNNNGG 2 cut(s) 322, 1106
AflIII ACRYGT 1 cut(s) 799
AgsI TTSAA 3 cut(s) 358, 467, 881
AhlI ACTAGT 1 cut(s) 1141
AjnI CCWGG 3 cut(s) 343, 1368, 1447
AjuI GAANNNNNNNTTGG 2 cut(s) 1272, 1304
AluBI AGCT 6 cut(s) 473, 490, 660, 825, 884, 1283
AluI AGCT 6 cut(s) 473, 490, 660, 825, 884, 1283
Alw26I GTCTC 4 cut(s) 301, 558, 699, 909
AlwI GGATC 2 cut(s) 1091, 1104
AlwNI CAGNNNCTG 2 cut(s) 590, 1283
ApeKI GCWGC 1 cut(s) 1031
ApoI RAATTY 3 cut(s) 818, 1124, 1312
AspS9I GGNCC 3 cut(s) 20, 525, 1445
AsuHPI GGTGA 5 cut(s) 262, 446, 605, 1166, 1355
AvaII GGWCC 1 cut(s) 20
AxyI CCTNAGG 1 cut(s) 1178
BalI TGGCCA 3 cut(s) 723, 861, 1269
BamHI GGATCC 1 cut(s) 1096
BanII GRGCYC 2 cut(s) 428, 796
BbrPI CACGTG 1 cut(s) 800
BbsI GAAGAC 2 cut(s) 100, 1041
BbvI GCAGC 1 cut(s) 1043
BccI CCATC 5 cut(s) 425, 489, 671, 882, 1078
BceAI ACGGC 1 cut(s) 525
BciT130I CCWGG 3 cut(s) 345, 1370, 1449
BciVI GTATCC 1 cut(s) 1232
BclI TGATCA 2 cut(s) 808, 1375
BcoDI GTCTC 4 cut(s) 301, 558, 699, 909
BcuI ACTAGT 1 cut(s) 1141
BfaI CTAG 3 cut(s) 927, 1121, 1142
BfmI CTRYAG 1 cut(s) 405
BfuAI ACCTGC 1 cut(s) 883
BfuI GTATCC 1 cut(s) 1232
BisI GCNGC 3 cut(s) 310, 313, 1032
BlsI GCNGC 3 cut(s) 311, 314, 1033
Bme1390I CCNGG 3 cut(s) 345, 1370, 1449
Bme18I GGWCC 1 cut(s) 20
BmgT120I GGNCC 3 cut(s) 20, 525, 1445
BmiI GGNNCC 3 cut(s) 795, 1098, 1184
BmrFI CCNGG 3 cut(s) 345, 1370, 1449
BmsI GCATC 3 cut(s) 943, 962, 1307
BpiI GAAGAC 2 cut(s) 100, 1041
Bpu10I CCTNAGC 1 cut(s) 486
BsaAI YACGTR 1 cut(s) 800
BsaBI GATNNNNATC 1 cut(s) 957
BsaJI CCNNGG 3 cut(s) 23, 1043, 1447
BsaXI ACNNNNNCTCC 4 cut(s) 41, 71, 1203, 1233
Bsc4I CCNNNNNNNGG 2 cut(s) 322, 1106
Bse118I RCCGGY 1 cut(s) 322
Bse1I ACTGG 4 cut(s) 183, 947, 973, 1118
Bse21I CCTNAGG 1 cut(s) 1178
Bse8I GATNNNNATC 1 cut(s) 957
BseBI CCWGG 3 cut(s) 345, 1370, 1449
BseDI CCNNGG 3 cut(s) 23, 1043, 1447
BseGI GGATG 6 cut(s) 198, 481, 517, 893, 977, 1378
BseJI GATNNNNATC 1 cut(s) 957
BseLI CCNNNNNNNGG 2 cut(s) 322, 1106
BseMII CTCAG 3 cut(s) 312, 477, 1169
BseNI ACTGG 4 cut(s) 183, 947, 973, 1118
BseRI GAGGAG 4 cut(s) 49, 715, 1195, 1224
BseX3I CGGCCG 1 cut(s) 307
BseXI GCAGC 1 cut(s) 1043
Bsh1285I CGRYCG 1 cut(s) 310
BsiEI CGRYCG 1 cut(s) 310
BsiSI CCGG 2 cut(s) 306, 323
BslFI GGGAC 1 cut(s) 1438
BslI CCNNNNNNNGG 2 cut(s) 322, 1106
BsmAI GTCTC 4 cut(s) 301, 558, 699, 909
BsmFI GGGAC 1 cut(s) 1438
BsmI GAATGC 1 cut(s) 1160
Bsp1286I GDGCHC 2 cut(s) 428, 796
Bsp143I GATC 7 cut(s) 393, 754, 808, 865, 1096, 1229, 1375
Bsp19I CCATGG 1 cut(s) 23
BspACI CCGC 2 cut(s) 310, 313
BspCNI CTCAG 3 cut(s) 311, 478, 1170
BspLI GGNNCC 3 cut(s) 795, 1098, 1184
BspMAI CTGCAG 1 cut(s) 409
BspMI ACCTGC 1 cut(s) 883
BspPI GGATC 2 cut(s) 1091, 1104
BspQI GCTCTTC 1 cut(s) 189
BsrFI RCCGGY 1 cut(s) 322
BsrI ACTGG 4 cut(s) 183, 947, 973, 1118
BssAI RCCGGY 1 cut(s) 322
BssECI CCNNGG 3 cut(s) 23, 1043, 1447
BssMI GATC 7 cut(s) 393, 754, 808, 865, 1096, 1229, 1375
BssT1I CCWWGG 2 cut(s) 23, 1043
Bst2UI CCWGG 3 cut(s) 345, 1370, 1449
Bst4CI ACNGT 6 cut(s) 124, 449, 569, 579, 616, 1109
Bst6I CTCTTC 2 cut(s) 189, 272
BstBAI YACGTR 1 cut(s) 800
BstC8I GCNNGC 7 cut(s) 324, 405, 658, 732, 778, 1158, 1192
BstDEI CTNAG 3 cut(s) 298, 486, 1178
BstDSI CCRYGG 1 cut(s) 23
BstF5I GGATG 6 cut(s) 198, 481, 517, 893, 977, 1378
BstKTI GATC 7 cut(s) 396, 757, 811, 868, 1099, 1232, 1378
BstMAI GTCTC 4 cut(s) 301, 558, 699, 909
BstMBI GATC 7 cut(s) 393, 754, 808, 865, 1096, 1229, 1375
BstMCI CGRYCG 1 cut(s) 310
BstMWI GCNNNNNNNGC 3 cut(s) 962, 1028, 1191
BstNI CCWGG 3 cut(s) 345, 1370, 1449
BstNSI RCATGY 1 cut(s) 734
BstSCI CCNGG 3 cut(s) 343, 1368, 1447
BstSFI CTRYAG 1 cut(s) 405
BstV1I GCAGC 1 cut(s) 1043
BstV2I GAAGAC 2 cut(s) 100, 1041
BstX2I RGATCY 1 cut(s) 1096
BstYI RGATCY 1 cut(s) 1096
BstZI CGGCCG 1 cut(s) 307
Bsu36I CCTNAGG 1 cut(s) 1178
BsuI GTATCC 1 cut(s) 1232
BtgI CCRYGG 1 cut(s) 23
BtsCI GGATG 6 cut(s) 198, 481, 517, 893, 977, 1378
BtsI GCAGTG 1 cut(s) 724
BtsIMutI CAGTG 3 cut(s) 724, 966, 1111
BveI ACCTGC 1 cut(s) 883
Cac8I GCNNGC 7 cut(s) 324, 405, 658, 732, 778, 1158, 1192
CaiI CAGNNNCTG 2 cut(s) 590, 1283
Cfr10I RCCGGY 1 cut(s) 322
Cfr13I GGNCC 3 cut(s) 20, 525, 1445
Csp6I GTAC 1 cut(s) 137
CviAII CATG 9 cut(s) 24, 63, 229, 725, 731, 863, 1187, 1195, 1470
CviQI GTAC 1 cut(s) 137
DdeI CTNAG 3 cut(s) 298, 486, 1178
DpnI GATC 7 cut(s) 395, 756, 810, 867, 1098, 1231, 1377
DpnII GATC 7 cut(s) 393, 754, 808, 865, 1096, 1229, 1375
DraI TTTAAA 1 cut(s) 387
EaeI YGGCCR 6 cut(s) 307, 324, 341, 721, 859, 1267
EagI CGGCCG 1 cut(s) 307
Eam1104I CTCTTC 2 cut(s) 189, 272
EarI CTCTTC 2 cut(s) 189, 272
EclXI CGGCCG 1 cut(s) 307
Eco130I CCWWGG 2 cut(s) 23, 1043
Eco24I GRGCYC 2 cut(s) 428, 796
Eco47I GGWCC 1 cut(s) 20
Eco52I CGGCCG 1 cut(s) 307
Eco57I CTGAAG 1 cut(s) 1291
Eco72I CACGTG 1 cut(s) 800
Eco81I CCTNAGG 1 cut(s) 1178
EcoO109I RGGNCCY 1 cut(s) 1445
EcoRII CCWGG 3 cut(s) 343, 1368, 1447
EcoT14I CCWWGG 2 cut(s) 23, 1043
EcoT22I ATGCAT 2 cut(s) 110, 958
EcoT38I GRGCYC 2 cut(s) 428, 796
ErhI CCWWGG 2 cut(s) 23, 1043
FaeI CATG 9 cut(s) 27, 66, 232, 728, 734, 866, 1190, 1198, 1473
FalI AAGNNNNNCTT 2 cut(s) 1036, 1068
FaqI GGGAC 1 cut(s) 1438
FatI CATG 9 cut(s) 23, 62, 228, 724, 730, 862, 1186, 1194, 1469
FbaI TGATCA 2 cut(s) 808, 1375
Fnu4HI GCNGC 3 cut(s) 310, 313, 1032
FokI GGATG 6 cut(s) 185, 468, 524, 900, 984, 1365
FriOI GRGCYC 2 cut(s) 428, 796
Fsp4HI GCNGC 3 cut(s) 310, 313, 1032
FspBI CTAG 3 cut(s) 927, 1121, 1142
GluI GCNGC 3 cut(s) 310, 313, 1032
HapII CCGG 2 cut(s) 306, 323
Hin1II CATG 9 cut(s) 27, 66, 232, 728, 734, 866, 1190, 1198, 1473
HinfI GANTC 5 cut(s) 302, 587, 596, 707, 930
HpaII CCGG 2 cut(s) 306, 323
HphI GGTGA 5 cut(s) 262, 446, 605, 1166, 1355
Hpy188I TCNGA 6 cut(s) 301, 333, 352, 586, 668, 1310
Hpy188III TCNNGA 4 cut(s) 412, 421, 485, 625
HpyAV CCTTC 1 cut(s) 1087
HpyCH4III ACNGT 6 cut(s) 124, 449, 569, 579, 616, 1109
HpyCH4IV ACGT 1 cut(s) 799
HpyF10VI GCNNNNNNNGC 3 cut(s) 962, 1028, 1191
HpyF3I CTNAG 3 cut(s) 298, 486, 1178
HpySE526I ACGT 1 cut(s) 799
Hsp92II CATG 9 cut(s) 27, 66, 232, 728, 734, 866, 1190, 1198, 1473
KroI GCCGGC 1 cut(s) 322
KroNI GCCGGC 1 cut(s) 324
Ksp22I TGATCA 2 cut(s) 808, 1375
Kzo9I GATC 7 cut(s) 393, 754, 808, 865, 1096, 1229, 1375
LguI GCTCTTC 1 cut(s) 189
LmnI GCTCC 5 cut(s) 665, 767, 799, 1028, 1182
Lsp1109I GCAGC 1 cut(s) 1043
LweI GCATC 3 cut(s) 943, 962, 1307
MaeI CTAG 3 cut(s) 927, 1121, 1142
MaeII ACGT 1 cut(s) 799
MaeIII GTNAC 6 cut(s) 282, 542, 802, 828, 1109, 1172
MalI GATC 7 cut(s) 395, 756, 810, 867, 1098, 1231, 1377
MboI GATC 7 cut(s) 393, 754, 808, 865, 1096, 1229, 1375
MfeI CAATTG 1 cut(s) 291
MflI RGATCY 1 cut(s) 1096
MhlI GDGCHC 2 cut(s) 428, 796
MlsI TGGCCA 3 cut(s) 723, 861, 1269
MluNI TGGCCA 3 cut(s) 723, 861, 1269
MlyI GAGTC 1 cut(s) 701
MmeI TCCRAC 1 cut(s) 1126
Mox20I TGGCCA 3 cut(s) 723, 861, 1269
Mph1103I ATGCAT 2 cut(s) 110, 958
MroNI GCCGGC 1 cut(s) 322
MscI TGGCCA 3 cut(s) 723, 861, 1269
MseI TTAA 3 cut(s) 386, 492, 650
MslI CAYNNNNRTG 2 cut(s) 729, 785
Msp20I TGGCCA 3 cut(s) 723, 861, 1269
MspI CCGG 2 cut(s) 306, 323
MspR9I CCNGG 3 cut(s) 345, 1370, 1449
MunI CAATTG 1 cut(s) 291
Mva1269I GAATGC 1 cut(s) 1160
MvaI CCWGG 3 cut(s) 345, 1370, 1449
MwoI GCNNNNNNNGC 3 cut(s) 962, 1028, 1191
NaeI GCCGGC 1 cut(s) 324
NcoI CCATGG 1 cut(s) 23
NdeII GATC 7 cut(s) 393, 754, 808, 865, 1096, 1229, 1375
NgoMIV GCCGGC 1 cut(s) 322
NlaIII CATG 9 cut(s) 27, 66, 232, 728, 734, 866, 1190, 1198, 1473
NlaIV GGNNCC 3 cut(s) 795, 1098, 1184
NmuCI GTSAC 5 cut(s) 282, 802, 828, 1109, 1172
NsiI ATGCAT 2 cut(s) 110, 958
NspI RCATGY 1 cut(s) 734
PaeI GCATGC 1 cut(s) 734
PciSI GCTCTTC 1 cut(s) 189
PctI GAATGC 1 cut(s) 1160
PdiI GCCGGC 1 cut(s) 324
PfeI GAWTC 4 cut(s) 302, 587, 596, 930
PkrI GCNGC 3 cut(s) 311, 314, 1033
PleI GAGTC 1 cut(s) 701
PmaCI CACGTG 1 cut(s) 800
PmlI CACGTG 1 cut(s) 800
PpsI GAGTC 1 cut(s) 701
Ppu21I YACGTR 1 cut(s) 800
Psp6I CCWGG 3 cut(s) 343, 1368, 1447
PspCI CACGTG 1 cut(s) 800
PspGI CCWGG 3 cut(s) 343, 1368, 1447
PspN4I GGNNCC 3 cut(s) 795, 1098, 1184
PspPI GGNCC 3 cut(s) 20, 525, 1445
PstI CTGCAG 1 cut(s) 409
PstNI CAGNNNCTG 2 cut(s) 590, 1283
PsuI RGATCY 1 cut(s) 1096
RsaI GTAC 1 cut(s) 138
RsaNI GTAC 1 cut(s) 137
RseI CAYNNNNRTG 2 cut(s) 729, 785
SapI GCTCTTC 1 cut(s) 189
SaqAI TTAA 3 cut(s) 386, 492, 650
SatI GCNGC 3 cut(s) 310, 313, 1032
Sau3AI GATC 7 cut(s) 393, 754, 808, 865, 1096, 1229, 1375
Sau96I GGNCC 3 cut(s) 20, 525, 1445
SchI GAGTC 1 cut(s) 701
ScrFI CCNGG 3 cut(s) 345, 1370, 1449
SduI GDGCHC 2 cut(s) 428, 796
SfaNI GCATC 3 cut(s) 943, 962, 1307
SfcI CTRYAG 1 cut(s) 405
SinI GGWCC 1 cut(s) 20
SmiMI CAYNNNNRTG 2 cut(s) 729, 785
SpeI ACTAGT 1 cut(s) 1141
SphI GCATGC 1 cut(s) 734
SsiI CCGC 2 cut(s) 310, 313
SspMI CTAG 3 cut(s) 927, 1121, 1142
StyD4I CCNGG 3 cut(s) 343, 1368, 1447
StyI CCWWGG 2 cut(s) 23, 1043
TaaI ACNGT 6 cut(s) 124, 449, 569, 579, 616, 1109
TaiI ACGT 1 cut(s) 802
TauI GCSGC 2 cut(s) 312, 315
TfiI GAWTC 4 cut(s) 302, 587, 596, 930
Tru1I TTAA 3 cut(s) 386, 492, 650
Tru9I TTAA 3 cut(s) 386, 492, 650
TscAI CASTG 3 cut(s) 724, 973, 1118
TseFI GTSAC 5 cut(s) 282, 802, 828, 1109, 1172
TseI GCWGC 1 cut(s) 1031
Tsp45I GTSAC 5 cut(s) 282, 802, 828, 1109, 1172
TspDTI ATGAA 5 cut(s) 17, 57, 528, 831, 843
TspRI CASTG 3 cut(s) 724, 973, 1118
VpaK11BI GGWCC 1 cut(s) 20
XapI RAATTY 3 cut(s) 818, 1124, 1312
XceI RCATGY 1 cut(s) 734
XcmI CCANNNNNNNNNTGG 1 cut(s) 428
XspI CTAG 3 cut(s) 927, 1121, 1142
Zsp2I ATGCAT 2 cut(s) 110, 958
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.