Rh7AG132600

Neprosin activation peptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0011754
Physical Location & Seq
Reverse (-)
10628724 .. 10632074
3351 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG132600.1

Sequence Viewer

Length: 1113 bp
ATGAAAGGAGCTTATTCAGCTGTTCTTGTTGGTTCTGCTCTGTCGACAAAAGAGAGGAATATTGAAGCAGAATCATCCTCATCATCTTCTTGTGCAATTCAGCACACAACAGGACAAGAGCAATGCATACAAGCACGTACTGCTGAAACATTACAAACAACTCCACTCAAACATTCCTCTAACCTGGTTGCTCATACTTTCCACAGCACCCCTTTCTCATTCGCATCCATGCTTCTTGTTCTCTTACTTCTTGTTGCTTTTGCTCCTGGACTTTCCTCCCTCCCCATTGCCTCGGATTCCGGGCACCGCCCACCGGCCAATCAGACATTCCGGCCTGGTGGGGAAGAGGTGCAGAACTTGAAGAGGGTCAGCTCTTATTTAAGCAAGATCAACAAGCCTGCTGTCAAGACTATAAAGAGTCCTGATGGTGATGTGATAGACTGTGTTTTAGCCCATCTCCAACCAGCTTTTGACCATCCTGAGCTTAAAGGCCAAAAACCATTGGATCTACCAGAAAGGCCTAAATCCCATAACAGCACGGATATAGTGGAAGAGGCTTATCAGCTATGGTCGGAATCCGGTGAGGCATGCCCTGAGGGAACTGTTCCGATTCGGAGGACCACAGACAAAGACATTTTGAGAGCAAGTTCAGTTCAAAGATTTGGAAGGAAATTACCAAGAAGACATGTGAAGAGGGACTCATCAGGCAGTGGTCATGAGCATGCAGTTGTTTTTGTAAATGGAGACCAGTACTATGGAGCAAAGGCCAGTATAAATGTGTGGTCGCCAAGAGTGACCGATCAATATGAATTTAGCTTATCACAGATTTGGGTCATCTCTGGTACATTTGGCCATGACCTAAATACCATTGAAGCTGGTTGGCAGGTTAGCCCAGAGTTATATGGAGACAACTACCCAAGATTCTTTACATATTGGACAACAGATGCATATCAAGCTACTGGATGCTACAACTTGCTGTGCTCTGGCTTTGTCCAAACCAATAATAAGATTGCTATTGGAGCTGCTATTTCTCCAAGGTCTTCCTTTAATGGCAGACAATTTGATATTGGCTTAATGGTTTGGAAGGTGAGTTTTAGTTCAGCATTCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

370

Amino Acids

40.66

Weight (kDa)

8.15

Isoelectric Point (pI)

45.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin_AP PF14365 137 - 244 8.4e-38 Neprosin activation peptide
Neprosin PF03080 269 - 363 2.8e-37 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011754)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50150
fragaria_vesca FvH4_5g05740
malus_domestica MD06G1170400.v1.1 MD14G1176000.v1.1
prunus_persica Prupe.5G170000_v2.0.a1 Prupe.5G170000_v2.0.a1
pyrus_communis pycom06g15180 pycom14g14710
rosa_chinensis RchiOBHm_Chr7g0193071
rosa_laevigata RLG00000004297
rosa_multiflora Rmu_ssc0000002.1_g000010
rosa_roxburghii Rroxscaffold_3G00262400
rosa_rugosa Rorug07G0007400
rosa_samantha Rh7AG132600 Rh7BG133500 Rh7CG136600 Rh7DG134500
rosa_wichuraiana Rw0G015850 Rw7G011330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 874
AccB1I GGYRCC 1 cut(s) 303
AccI GTMKAC 1 cut(s) 44
AciI CCGC 1 cut(s) 307
AclWI GGATC 1 cut(s) 513
AcoI YGGCCR 2 cut(s) 315, 850
AcsI RAATTY 1 cut(s) 809
AfaI GTAC 3 cut(s) 139, 752, 844
AfiI CCNNNNNNNGG 1 cut(s) 313
AflIII ACRYGT 1 cut(s) 685
AgsI TTSAA 5 cut(s) 65, 361, 656, 872, 1108
AjnI CCWGG 3 cut(s) 183, 265, 334
AjuI GAANNNNNNNTTGG 2 cut(s) 311, 343
Alw21I GWGCWC 1 cut(s) 983
Alw26I GTCTC 2 cut(s) 738, 900
AlwI GGATC 1 cut(s) 513
AoxI GGCC 6 cut(s) 315, 332, 490, 518, 765, 850
ApeKI GCWGC 1 cut(s) 1022
ApoI RAATTY 1 cut(s) 809
AspS9I GGNCC 1 cut(s) 618
AsuC2I CCSGG 1 cut(s) 301
AsuHPI GGTGA 3 cut(s) 440, 593, 1099
AvaII GGWCC 1 cut(s) 618
AxyI CCTNAGG 1 cut(s) 594
BaeGI GKGCMC 1 cut(s) 306
BalI TGGCCA 1 cut(s) 852
BanI GGYRCC 1 cut(s) 303
BbsI GAAGAC 2 cut(s) 688, 1032
Bbv12I GWGCWC 1 cut(s) 983
BbvI GCAGC 1 cut(s) 1009
BccI CCATC 3 cut(s) 419, 462, 483
BciT130I CCWGG 3 cut(s) 185, 267, 336
BcnI CCSGG 1 cut(s) 301
BcoDI GTCTC 2 cut(s) 738, 900
BfuAI ACCTGC 1 cut(s) 874
BisI GCNGC 1 cut(s) 1023
BlsI GCNGC 1 cut(s) 1024
BmcAI AGTACT 1 cut(s) 752
Bme1390I CCNGG 4 cut(s) 185, 267, 301, 336
Bme18I GGWCC 1 cut(s) 618
BmgT120I GGNCC 1 cut(s) 618
BmiI GGNNCC 1 cut(s) 305
BmrFI CCNGG 4 cut(s) 185, 267, 301, 336
BmsI GCATC 3 cut(s) 233, 934, 953
BpiI GAAGAC 2 cut(s) 688, 1032
Bpu10I CCTNAGC 1 cut(s) 480
BpuMI CCSGG 1 cut(s) 301
BsaAI YACGTR 1 cut(s) 137
BsaBI GATNNNNATC 1 cut(s) 948
BsaI GGTCTC 1 cut(s) 738
BsaJI CCNNGG 2 cut(s) 291, 1034
BsaWI WCCGGW 1 cut(s) 578
Bsc4I CCNNNNNNNGG 1 cut(s) 313
Bse118I RCCGGY 1 cut(s) 313
Bse1I ACTGG 3 cut(s) 748, 768, 964
Bse21I CCTNAGG 1 cut(s) 594
Bse3DI GCAATG 2 cut(s) 128, 285
Bse8I GATNNNNATC 1 cut(s) 948
BseBI CCWGG 3 cut(s) 185, 267, 336
BseDI CCNNGG 2 cut(s) 291, 1034
BseGI GGATG 4 cut(s) 74, 224, 475, 968
BseJI GATNNNNATC 1 cut(s) 948
BseLI CCNNNNNNNGG 1 cut(s) 313
BseMI GCAATG 2 cut(s) 128, 285
BseMII CTCAG 2 cut(s) 471, 585
BseNI ACTGG 3 cut(s) 748, 768, 964
BseSI GKGCMC 1 cut(s) 306
BseXI GCAGC 1 cut(s) 1009
BsgI GTGCAG 1 cut(s) 371
BshFI GGCC 6 cut(s) 317, 334, 492, 520, 767, 852
BshNI GGYRCC 1 cut(s) 303
BsiHKAI GWGCWC 1 cut(s) 983
BsiSI CCGG 4 cut(s) 300, 314, 331, 579
BslFI GGGAC 1 cut(s) 710
BslI CCNNNNNNNGG 1 cut(s) 313
BsmAI GTCTC 2 cut(s) 738, 900
BsmFI GGGAC 1 cut(s) 710
BsmI GAATGC 1 cut(s) 1103
BsnI GGCC 6 cut(s) 317, 334, 492, 520, 767, 852
Bso31I GGTCTC 1 cut(s) 738
Bsp1286I GDGCHC 2 cut(s) 306, 983
Bsp143I GATC 3 cut(s) 387, 505, 799
BspACI CCGC 1 cut(s) 307
BspANI GGCC 6 cut(s) 317, 334, 492, 520, 767, 852
BspCNI CTCAG 2 cut(s) 472, 586
BspHI TCATGA 1 cut(s) 715
BspLI GGNNCC 1 cut(s) 305
BspMI ACCTGC 1 cut(s) 874
BspPI GGATC 1 cut(s) 513
BspT107I GGYRCC 1 cut(s) 303
BspTNI GGTCTC 1 cut(s) 738
BsrDI GCAATG 2 cut(s) 128, 285
BsrFI RCCGGY 1 cut(s) 313
BsrI ACTGG 3 cut(s) 748, 768, 964
BssAI RCCGGY 1 cut(s) 313
BssECI CCNNGG 2 cut(s) 291, 1034
BssMI GATC 3 cut(s) 387, 505, 799
BssT1I CCWWGG 1 cut(s) 1034
Bst2UI CCWGG 3 cut(s) 185, 267, 336
Bst4CI ACNGT 2 cut(s) 443, 604
Bst6I CTCTTC 4 cut(s) 339, 356, 546, 686
BstAPI GCANNNNNTGC 1 cut(s) 140
BstBAI YACGTR 1 cut(s) 137
BstC8I GCNNGC 3 cut(s) 399, 589, 723
BstDEI CTNAG 2 cut(s) 480, 594
BstF5I GGATG 4 cut(s) 74, 224, 475, 968
BstKTI GATC 3 cut(s) 390, 508, 802
BstMAI GTCTC 2 cut(s) 738, 900
BstMBI GATC 3 cut(s) 387, 505, 799
BstMWI GCNNNNNNNGC 4 cut(s) 17, 140, 953, 1019
BstNI CCWGG 3 cut(s) 185, 267, 336
BstNSI RCATGY 3 cut(s) 591, 689, 725
BstSCI CCNGG 4 cut(s) 183, 265, 299, 334
BstSLI GKGCMC 1 cut(s) 306
BstV1I GCAGC 1 cut(s) 1009
BstV2I GAAGAC 2 cut(s) 688, 1032
BstX2I RGATCY 1 cut(s) 505
BstXI CCANNNNNNTGG 1 cut(s) 755
BstYI RGATCY 1 cut(s) 505
Bsu36I CCTNAGG 1 cut(s) 594
BsuRI GGCC 6 cut(s) 317, 334, 492, 520, 767, 852
BtsCI GGATG 4 cut(s) 74, 224, 475, 968
BtsI GCAGTG 1 cut(s) 715
BtsIMutI CAGTG 1 cut(s) 715
BveI ACCTGC 1 cut(s) 874
Cac8I GCNNGC 3 cut(s) 399, 589, 723
CciI TCATGA 1 cut(s) 715
Cfr10I RCCGGY 1 cut(s) 313
Cfr13I GGNCC 1 cut(s) 618
CsiI ACCWGGT 1 cut(s) 183
Csp6I GTAC 3 cut(s) 138, 751, 843
CviAII CATG 6 cut(s) 229, 588, 686, 716, 722, 854
CviQI GTAC 3 cut(s) 138, 751, 843
DdeI CTNAG 2 cut(s) 480, 594
DpnI GATC 3 cut(s) 389, 507, 801
DpnII GATC 3 cut(s) 387, 505, 799
EaeI YGGCCR 2 cut(s) 315, 850
Eam1104I CTCTTC 4 cut(s) 339, 356, 546, 686
EarI CTCTTC 4 cut(s) 339, 356, 546, 686
Eco130I CCWWGG 1 cut(s) 1034
Eco147I AGGCCT 1 cut(s) 520
Eco31I GGTCTC 1 cut(s) 738
Eco47I GGWCC 1 cut(s) 618
Eco81I CCTNAGG 1 cut(s) 594
EcoRII CCWGG 3 cut(s) 183, 265, 334
EcoT14I CCWWGG 1 cut(s) 1034
EcoT22I ATGCAT 2 cut(s) 128, 949
ErhI CCWWGG 1 cut(s) 1034
FaeI CATG 6 cut(s) 232, 591, 689, 719, 725, 857
FaqI GGGAC 1 cut(s) 710
FatI CATG 6 cut(s) 228, 587, 685, 715, 721, 853
FblI GTMKAC 1 cut(s) 44
Fnu4HI GCNGC 1 cut(s) 1023
FokI GGATG 4 cut(s) 61, 211, 462, 975
Fsp4HI GCNGC 1 cut(s) 1023
GluI GCNGC 1 cut(s) 1023
HaeIII GGCC 6 cut(s) 317, 334, 492, 520, 767, 852
HapII CCGG 4 cut(s) 300, 314, 331, 579
Hin1II CATG 6 cut(s) 232, 591, 689, 719, 725, 857
HincII GTYRAC 1 cut(s) 45
HindII GTYRAC 1 cut(s) 45
HinfI GANTC 7 cut(s) 71, 296, 418, 575, 610, 698, 921
HpaII CCGG 4 cut(s) 300, 314, 331, 579
HphI GGTGA 3 cut(s) 440, 593, 1099
Hpy166II GTNNAC 1 cut(s) 45
Hpy188I TCNGA 5 cut(s) 295, 324, 574, 609, 615
Hpy188III TCNNGA 4 cut(s) 406, 422, 479, 716
Hpy8I GTNNAC 1 cut(s) 45
HpyAV CCTTC 2 cut(s) 660, 1078
HpyCH4III ACNGT 2 cut(s) 443, 604
HpyCH4IV ACGT 1 cut(s) 136
HpyCH4V TGCA 5 cut(s) 95, 126, 352, 725, 947
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 140, 953, 1019
HpyF3I CTNAG 2 cut(s) 480, 594
HpySE526I ACGT 1 cut(s) 136
Hsp92II CATG 6 cut(s) 232, 591, 689, 719, 725, 857
Kzo9I GATC 3 cut(s) 387, 505, 799
LmnI GCTCC 4 cut(s) 8, 268, 758, 1019
Lsp1109I GCAGC 1 cut(s) 1009
LweI GCATC 3 cut(s) 233, 934, 953
MabI ACCWGGT 1 cut(s) 183
MaeII ACGT 1 cut(s) 136
MaeIII GTNAC 1 cut(s) 793
MalI GATC 3 cut(s) 389, 507, 801
MboI GATC 3 cut(s) 387, 505, 799
MboII GAAGA 7 cut(s) 78, 356, 373, 563, 693, 703, 1032
MflI RGATCY 1 cut(s) 505
MhlI GDGCHC 2 cut(s) 306, 983
MlsI TGGCCA 1 cut(s) 852
MluCI AATT 4 cut(s) 96, 671, 809, 1058
MluNI TGGCCA 1 cut(s) 852
MlyI GAGTC 2 cut(s) 427, 692
MmeI TCCRAC 2 cut(s) 484, 552
Mox20I TGGCCA 1 cut(s) 852
Mph1103I ATGCAT 2 cut(s) 128, 949
MscI TGGCCA 1 cut(s) 852
MseI TTAA 4 cut(s) 380, 486, 1047, 1073
MslI CAYNNNNRTG 1 cut(s) 720
Msp20I TGGCCA 1 cut(s) 852
MspA1I CMGCKG 1 cut(s) 20
MspI CCGG 4 cut(s) 300, 314, 331, 579
MspR9I CCNGG 4 cut(s) 185, 267, 301, 336
Mva1269I GAATGC 1 cut(s) 1103
MvaI CCWGG 3 cut(s) 185, 267, 336
MwoI GCNNNNNNNGC 4 cut(s) 17, 140, 953, 1019
NciI CCSGG 1 cut(s) 301
NdeII GATC 3 cut(s) 387, 505, 799
NlaIII CATG 6 cut(s) 232, 591, 689, 719, 725, 857
NlaIV GGNNCC 1 cut(s) 305
NmuCI GTSAC 1 cut(s) 793
NsiI ATGCAT 2 cut(s) 128, 949
NspI RCATGY 3 cut(s) 591, 689, 725
PaeI GCATGC 2 cut(s) 591, 725
PagI TCATGA 1 cut(s) 715
PceI AGGCCT 1 cut(s) 520
PciI ACATGT 1 cut(s) 685
PctI GAATGC 1 cut(s) 1103
PfeI GAWTC 5 cut(s) 71, 296, 575, 610, 921
PfoI TCCNGGA 1 cut(s) 265
PkrI GCNGC 1 cut(s) 1024
PleI GAGTC 2 cut(s) 426, 692
PpsI GAGTC 2 cut(s) 426, 692
Ppu21I YACGTR 1 cut(s) 137
PscI ACATGT 1 cut(s) 685
Psp6I CCWGG 3 cut(s) 183, 265, 334
PspGI CCWGG 3 cut(s) 183, 265, 334
PspN4I GGNNCC 1 cut(s) 305
PspPI GGNCC 1 cut(s) 618
PsuI RGATCY 1 cut(s) 505
PvuII CAGCTG 1 cut(s) 20
RsaI GTAC 3 cut(s) 139, 752, 844
RsaNI GTAC 3 cut(s) 138, 751, 843
RseI CAYNNNNRTG 1 cut(s) 720
SalI GTCGAC 1 cut(s) 43
SaqAI TTAA 4 cut(s) 380, 486, 1047, 1073
SatI GCNGC 1 cut(s) 1023
Sau3AI GATC 3 cut(s) 387, 505, 799
Sau96I GGNCC 1 cut(s) 618
ScaI AGTACT 1 cut(s) 752
SchI GAGTC 2 cut(s) 427, 692
ScrFI CCNGG 4 cut(s) 185, 267, 301, 336
SduI GDGCHC 2 cut(s) 306, 983
SexAI ACCWGGT 1 cut(s) 183
SfaNI GCATC 3 cut(s) 233, 934, 953
SinI GGWCC 1 cut(s) 618
SmiMI CAYNNNNRTG 1 cut(s) 720
SphI GCATGC 2 cut(s) 591, 725
Sse9I AATT 4 cut(s) 96, 671, 809, 1058
SseBI AGGCCT 1 cut(s) 520
SsiI CCGC 1 cut(s) 307
SspI AATATT 1 cut(s) 61
StuI AGGCCT 1 cut(s) 520
StyD4I CCNGG 4 cut(s) 183, 265, 299, 334
StyI CCWWGG 1 cut(s) 1034
TaaI ACNGT 2 cut(s) 443, 604
TaiI ACGT 1 cut(s) 139
TaqI TCGA 1 cut(s) 44
TaqII GACCGA 1 cut(s) 812
TasI AATT 4 cut(s) 96, 671, 809, 1058
TatI WGTACW 1 cut(s) 750
TfiI GAWTC 5 cut(s) 71, 296, 575, 610, 921
Tru1I TTAA 4 cut(s) 380, 486, 1047, 1073
Tru9I TTAA 4 cut(s) 380, 486, 1047, 1073
TscAI CASTG 1 cut(s) 715
TseFI GTSAC 1 cut(s) 793
TseI GCWGC 1 cut(s) 1022
Tsp45I GTSAC 1 cut(s) 793
TspDTI ATGAA 2 cut(s) 17, 822
TspGWI ACGGA 1 cut(s) 554
TspRI CASTG 1 cut(s) 715
VpaK11BI GGWCC 1 cut(s) 618
XapI RAATTY 1 cut(s) 809
XceI RCATGY 3 cut(s) 591, 689, 725
XmiI GTMKAC 1 cut(s) 44
ZrmI AGTACT 1 cut(s) 752
Zsp2I ATGCAT 2 cut(s) 128, 949
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.