MD06G1188700.v1.1

Isochorismate synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0009008
Physical Location & Seq
Reverse (-)
32575998 .. 32580440
4443 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1188700.v1.1.491

Sequence Viewer

Length: 1683 bp
ATGGCCACAGCTCAAGCAGCTGCAGCTGCTCGGCCATGTTTCATGGATCCCGAAGCAGCCGCCAAATGCAGCATCACAGTCTCCAGCAGGCAAACCATTCATTTCTCGCACCATAGGTCTCAGTTGTGTTCACTTTCCATGAATGGTTGCCAAGGAGAATCTCACCTCCCTTTAGGCACCGTTGAAACGCGCTCGTTTCCGGCGGTTCCATCACCTTCATTGGCTCTGGACCGGCTCAGCTCCGCCATTTCGGAACTGAAATCAAACCCGCCTCCGTCTTCCTCCGGAATTATACGTCTCCAGGTGCCAATCCAGCAGCAAATTGAAGCAATCGACTGGCTTTACGCCCAAGACCAATTCCTTCCGCGCTGTTTCTTCTCCGGCAGATGCCAAACTGGTCATTTTTCTGATCTATCCTTTGACTACACCAATGGAAATGACCAGAATCAATCAAAACACAAGTTGGTCAGCGTTGCCGGCGTGGGGTCTGCAGTTTTCTTTCAGGATGTGCATCCCTTCTCGTACAGGCATTGGAAGTCCTTAAAGAGGTTCCTCTCTACAGAATGTCCACTTATTCGCGCTTATGGGGCAATCCGTTTCGATGCAAGAGCCAACATATCATCTGAATGGGAGGCATTTGGTTACTTTTATTTTATGATTCCTCAGGTTGAGTTTGATGAGCTTGAAGAAGGTTCAATGCTAGCAACAACCATTGCGTGGGACAATGCGCTTTCGTGGAATTTAGGACATGCAATTGATGCACTTAAAGCTACGATGCATCAGATATCTTCCATTGTTTTAAAGCTTCGAAGACAAGTTCCTTCGACACTAATGTTAAGGAATAATAACATCCCGTGCAAGACGTATTGGGATCTTGCTGTTAATAGCGCTTTGCAGAAAATAAGAAGCAACTCGGCACTTATTAAGGTGGTACTCGCGCGTAGCAGCAGAGTAATAACAGCTACTGATATTGATCCCATAGCATGGCTAGCGTGCTTACAGGTTGAAGGTGAAAATGCTTATCAGTTTTGTCTTCAGCCGCCAAATGCACCAGCGTTTATTGGAAACACGCCAGAGCAACTGTTTCACAGAAAATGGCTAGGCATTACTAGCGAGGCTCTGGCTGGAACCCGAGCCAGAGGTGAATCGATGCCTGAAGATCTTGAAATAGAACTCGAGTTACTTTCCAGTCCAAAGGACCACCTTGAGTTTACCATTGTACGAGAAAGCATACAACGAAAACTAGAGAATGTATGTGACAGTGTCCAAGTTGAACCAAAGAAAGCAATCCGTAAATTCCCTCGAGTCCAACATTTATATGCTAAATTGGCTGGCAGGTTAAGAAGTGAAGATGATGAGTTTGACATATTGTCTTCTCTTCACCCAAGTCCAGCAGTTTGTGGATTTCCAACAGAGGAGGCACGGCTTTTAATTGCGGAAACAGAAGTATTCGACCGAGGGATGTATGCTGGGCCGGTTGGTTGGTTTGGAGGAGGAGAGAGTGAGTTTGCGGTTGGCATTAGGTCAGCATTAGTGGAAAAGGGTCTCGGCGCGATGATCTATGCTGGAACTGGGGTTGTGGAAGGAAGTAATGCATCGTTAGAATGGGATGAACTCGAACTCAAGATTTCTCAGTTCACCAAGTTGCTTAAACTTGAAGTGCCTCTGGAGATTAAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002376 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0006082 GO:0006575 GO:0006725 GO:0006732 GO:0006760 GO:0006766 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0008483 GO:0008909 GO:0009058 GO:0009108 GO:0009110 GO:0009266 GO:0009396 GO:0009409 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009627 GO:0009628 GO:0009696 GO:0009697 GO:0009814 GO:0009987 GO:0010118 GO:0010817 GO:0016020 GO:0016053 GO:0016740 GO:0016769 GO:0016853 GO:0016866 GO:0016999 GO:0017000 GO:0017144 GO:0018130 GO:0018958 GO:0019438 GO:0019752 GO:0019867 GO:0031090 GO:0031347 GO:0031348 GO:0031966 GO:0031967 GO:0031968 GO:0031975 GO:0032787 GO:0034641 GO:0042180 GO:0042181 GO:0042372 GO:0042374 GO:0042398 GO:0042445 GO:0042446 GO:0042537 GO:0042558 GO:0042559 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046189 GO:0046394 GO:0046483 GO:0046653 GO:0046654 GO:0046820 GO:0048519 GO:0048583 GO:0048585 GO:0050486 GO:0050789 GO:0050832 GO:0050896 GO:0051186 GO:0051188 GO:0051704 GO:0051707 GO:0065007 GO:0065008 GO:0071704 GO:0072330 GO:0080134 GO:0098542 GO:0098588 GO:0098805 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901615 GO:1901617 GO:1901661 GO:1901663
KEGG Pathways
Metabolic & Signaling
ko00130 ko01053 ko01100 ko01110 ko01130 ko04020 ko04022 ko04216 ko04217 ko04218 ko04621 ko04979 ko05012 ko05016 ko05166 map00130 map01053 map01100 map01110 map01130 map04020 map04022 map04216 map04217 map04218 map04621 map04979 map05012 map05016 map05166
Pfam Domains
Protein Families
Chorismate_bind Porin_3

Protein Analysis

561

Amino Acids

62.14

Weight (kDa)

5.92

Isoelectric Point (pI)

50.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chorismate_bind PF00425 290 - 542 1.3e-62 chorismate binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1326
AccB1I GGYRCC 2 cut(s) 176, 304
AccB7I CCANNNNNTGG 2 cut(s) 717, 984
AccII CGCG 6 cut(s) 190, 367, 579, 938, 940, 1553
AccIII TCCGGA 1 cut(s) 284
AciI CCGC 8 cut(s) 60, 203, 243, 269, 365, 1040, 1436, 1511
AclWI GGATC 4 cut(s) 41, 54, 879, 968
AcoI YGGCCR 2 cut(s) 3, 32
AcsI RAATTY 2 cut(s) 739, 1295
AcuI CTGAAG 2 cut(s) 1019, 1176
AfaI GTAC 3 cut(s) 524, 933, 1221
AfeI AGCGCT 1 cut(s) 889
AfiI CCNNNNNNNGG 4 cut(s) 483, 546, 717, 984
AgsI TTSAA 8 cut(s) 185, 326, 686, 696, 1007, 1166, 1274, 1658
AhdI GACNNNNNGTC 1 cut(s) 1369
AjnI CCWGG 1 cut(s) 300
AluBI AGCT 9 cut(s) 11, 20, 26, 240, 682, 770, 805, 962, 1680
AluI AGCT 9 cut(s) 11, 20, 26, 240, 682, 770, 805, 962, 1680
Alw26I GTCTC 4 cut(s) 85, 123, 302, 1550
AlwI GGATC 4 cut(s) 41, 54, 879, 968
AlwNI CAGNNNCTG 1 cut(s) 965
Ama87I CYCGRG 3 cut(s) 1131, 1175, 1302
Aor13HI TCCGGA 1 cut(s) 284
Aor51HI AGCGCT 1 cut(s) 889
AoxI GGCC 3 cut(s) 3, 32, 1472
ApeKI GCWGC 8 cut(s) 17, 20, 23, 26, 56, 69, 316, 945
ApoI RAATTY 2 cut(s) 739, 1295
AspLEI GCGC 7 cut(s) 192, 369, 581, 730, 890, 940, 1553
AspS9I GGNCC 3 cut(s) 229, 1198, 1472
AsuHPI GGTGA 6 cut(s) 155, 204, 1022, 1154, 1373, 1630
AsuII TTCGAA 1 cut(s) 808
AsuNHI GCTAGC 2 cut(s) 700, 988
AvaI CYCGRG 3 cut(s) 1131, 1175, 1302
AvaII GGWCC 2 cut(s) 229, 1198
AxyI CCTNAGG 1 cut(s) 663
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 46
BanI GGYRCC 2 cut(s) 176, 304
BbsI GAAGAC 4 cut(s) 270, 817, 1025, 1365
BbvI GCAGC 8 cut(s) 7, 13, 29, 35, 68, 81, 328, 957
BccI CCATC 1 cut(s) 217
BceAI ACGGC 1 cut(s) 1439
BciT130I CCWGG 1 cut(s) 302
BcoDI GTCTC 4 cut(s) 85, 123, 302, 1550
BfaI CTAG 5 cut(s) 701, 989, 1100, 1110, 1244
BfmI CTRYAG 3 cut(s) 21, 489, 558
BfoI RGCGCY 1 cut(s) 891
BfuAI ACCTGC 1 cut(s) 1326
BglII AGATCT 1 cut(s) 1159
BlpI GCTNAGC 1 cut(s) 236
Bme1390I CCNGG 1 cut(s) 302
Bme18I GGWCC 2 cut(s) 229, 1198
BmeRI GACNNNNNGTC 1 cut(s) 1369
BmeT110I CYCGRG 3 cut(s) 1131, 1175, 1302
BmgT120I GGNCC 3 cut(s) 229, 1198, 1472
BmiI GGNNCC 6 cut(s) 48, 178, 207, 306, 551, 1129
BmrFI CCNGG 1 cut(s) 302
BmrI ACTGGG 1 cut(s) 1581
BmsI GCATC 9 cut(s) 81, 377, 520, 592, 748, 765, 787, 1140, 1604
BmtI GCTAGC 2 cut(s) 704, 992
BmuI ACTGGG 1 cut(s) 1581
BpiI GAAGAC 4 cut(s) 270, 817, 1025, 1365
BpmI CTGGAG 2 cut(s) 67, 284
Bpu1102I GCTNAGC 1 cut(s) 236
Bpu14I TTCGAA 1 cut(s) 808
BpuEI CTTGAG 2 cut(s) 1226, 1607
Bsa29I ATCGAT 1 cut(s) 1148
BsaBI GATNNNNATC 2 cut(s) 510, 972
BsaI GGTCTC 2 cut(s) 123, 1550
BsaJI CCNNGG 2 cut(s) 151, 1456
BsaWI WCCGGW 1 cut(s) 284
Bsc4I CCNNNNNNNGG 4 cut(s) 483, 546, 717, 984
Bse118I RCCGGY 3 cut(s) 231, 476, 1474
Bse1I ACTGG 4 cut(s) 341, 400, 1188, 1576
Bse21I CCTNAGG 1 cut(s) 663
Bse3DI GCAATG 1 cut(s) 711
Bse8I GATNNNNATC 2 cut(s) 510, 972
BseAI TCCGGA 1 cut(s) 284
BseBI CCWGG 1 cut(s) 302
BseCI ATCGAT 1 cut(s) 1148
BseDI CCNNGG 2 cut(s) 151, 1456
BseGI GGATG 5 cut(s) 511, 511, 849, 1467, 1615
BseJI GATNNNNATC 2 cut(s) 510, 972
BseLI CCNNNNNNNGG 4 cut(s) 483, 546, 717, 984
BseMI GCAATG 1 cut(s) 711
BseMII CTCAG 4 cut(s) 134, 250, 677, 1646
BseNI ACTGG 4 cut(s) 341, 400, 1188, 1576
BseRI GAGGAG 3 cut(s) 1430, 1506, 1509
BseXI GCAGC 8 cut(s) 7, 13, 29, 35, 68, 81, 328, 957
BseYI CCCAGC 1 cut(s) 1469
Bsh1236I CGCG 6 cut(s) 190, 367, 579, 938, 940, 1553
Bsh1285I CGRYCG 1 cut(s) 1456
BshFI GGCC 3 cut(s) 5, 34, 1474
BshNI GGYRCC 2 cut(s) 176, 304
BshVI ATCGAT 1 cut(s) 1148
BsiEI CGRYCG 1 cut(s) 1456
BsiHKCI CYCGRG 3 cut(s) 1131, 1175, 1302
BsiSI CCGG 6 cut(s) 200, 232, 285, 381, 477, 1475
BslFI GGGAC 1 cut(s) 734
BslI CCNNNNNNNGG 4 cut(s) 483, 546, 717, 984
BsmAI GTCTC 4 cut(s) 85, 123, 302, 1550
BsmBI CGTCTC 1 cut(s) 302
BsmFI GGGAC 1 cut(s) 734
BsnI GGCC 3 cut(s) 5, 34, 1474
Bso31I GGTCTC 2 cut(s) 123, 1550
BsoBI CYCGRG 3 cut(s) 1131, 1175, 1302
Bsp119I TTCGAA 1 cut(s) 808
Bsp13I TCCGGA 1 cut(s) 284
Bsp143I GATC 6 cut(s) 46, 409, 871, 973, 1159, 1557
Bsp1720I GCTNAGC 1 cut(s) 236
BspACI CCGC 8 cut(s) 60, 203, 243, 269, 365, 1040, 1436, 1511
BspANI GGCC 3 cut(s) 5, 34, 1474
BspCNI CTCAG 4 cut(s) 133, 249, 676, 1645
BspDI ATCGAT 1 cut(s) 1148
BspEI TCCGGA 1 cut(s) 284
BspFNI CGCG 6 cut(s) 190, 367, 579, 938, 940, 1553
BspLI GGNNCC 6 cut(s) 48, 178, 207, 306, 551, 1129
BspMAI CTGCAG 2 cut(s) 25, 493
BspMI ACCTGC 1 cut(s) 1326
BspOI GCTAGC 2 cut(s) 704, 992
BspPI GGATC 4 cut(s) 41, 54, 879, 968
BspT104I TTCGAA 1 cut(s) 808
BspT107I GGYRCC 2 cut(s) 176, 304
BspTNI GGTCTC 2 cut(s) 123, 1550
BsrDI GCAATG 1 cut(s) 711
BsrFI RCCGGY 3 cut(s) 231, 476, 1474
BsrI ACTGG 4 cut(s) 341, 400, 1188, 1576
BssAI RCCGGY 3 cut(s) 231, 476, 1474
BssECI CCNNGG 2 cut(s) 151, 1456
BssMI GATC 6 cut(s) 46, 409, 871, 973, 1159, 1557
BssT1I CCWWGG 1 cut(s) 151
Bst2UI CCWGG 1 cut(s) 302
Bst4CI ACNGT 4 cut(s) 79, 181, 1083, 1262
Bst6I CTCTTC 1 cut(s) 1383
BstAPI GCANNNNNTGC 1 cut(s) 758
BstBI TTCGAA 1 cut(s) 808
BstC8I GCNNGC 6 cut(s) 89, 478, 702, 990, 994, 1333
BstDEI CTNAG 4 cut(s) 120, 236, 663, 1632
BstENI CCTNNNNNAGG 1 cut(s) 544
BstF5I GGATG 5 cut(s) 511, 511, 849, 1467, 1615
BstFNI CGCG 6 cut(s) 190, 367, 579, 938, 940, 1553
BstH2I RGCGCY 1 cut(s) 891
BstHHI GCGC 7 cut(s) 192, 369, 581, 730, 890, 940, 1553
BstKTI GATC 6 cut(s) 49, 412, 874, 976, 1162, 1560
BstMAI GTCTC 4 cut(s) 85, 123, 302, 1550
BstMBI GATC 6 cut(s) 46, 409, 871, 973, 1159, 1557
BstMCI CGRYCG 1 cut(s) 1456
BstNI CCWGG 1 cut(s) 302
BstNSI RCATGY 1 cut(s) 752
BstSCI CCNGG 1 cut(s) 300
BstSFI CTRYAG 3 cut(s) 21, 489, 558
BstUI CGCG 6 cut(s) 190, 367, 579, 938, 940, 1553
BstV1I GCAGC 8 cut(s) 7, 13, 29, 35, 68, 81, 328, 957
BstV2I GAAGAC 4 cut(s) 270, 817, 1025, 1365
BstX2I RGATCY 3 cut(s) 46, 871, 1159
BstYI RGATCY 3 cut(s) 46, 871, 1159
Bsu15I ATCGAT 1 cut(s) 1148
Bsu36I CCTNAGG 1 cut(s) 663
BsuRI GGCC 3 cut(s) 5, 34, 1474
BsuTUI ATCGAT 1 cut(s) 1148
BtgZI GCGATG 1 cut(s) 1568
BtsCI GGATG 5 cut(s) 511, 511, 849, 1467, 1615
BtsIMutI CAGTG 1 cut(s) 1267
BveI ACCTGC 1 cut(s) 1326
Cac8I GCNNGC 6 cut(s) 89, 478, 702, 990, 994, 1333
CaiI CAGNNNCTG 1 cut(s) 965
CfoI GCGC 7 cut(s) 192, 369, 581, 730, 890, 940, 1553
Cfr10I RCCGGY 3 cut(s) 231, 476, 1474
Cfr13I GGNCC 3 cut(s) 229, 1198, 1472
ClaI ATCGAT 1 cut(s) 1148
Csp6I GTAC 3 cut(s) 523, 932, 1220
CviAII CATG 5 cut(s) 36, 43, 139, 749, 984
CviQI GTAC 3 cut(s) 523, 932, 1220
DdeI CTNAG 4 cut(s) 120, 236, 663, 1632
DpnI GATC 6 cut(s) 48, 411, 873, 975, 1161, 1559
DpnII GATC 6 cut(s) 46, 409, 871, 973, 1159, 1557
DraI TTTAAA 1 cut(s) 801
DriI GACNNNNNGTC 1 cut(s) 1369
EaeI YGGCCR 2 cut(s) 3, 32
Eam1104I CTCTTC 1 cut(s) 1383
Eam1105I GACNNNNNGTC 1 cut(s) 1369
EarI CTCTTC 1 cut(s) 1383
EciI GGCGGA 1 cut(s) 232
Eco130I CCWWGG 1 cut(s) 151
Eco31I GGTCTC 2 cut(s) 123, 1550
Eco32I GATATC 1 cut(s) 786
Eco47I GGWCC 2 cut(s) 229, 1198
Eco47III AGCGCT 1 cut(s) 889
Eco57I CTGAAG 2 cut(s) 1019, 1176
Eco81I CCTNAGG 1 cut(s) 663
Eco88I CYCGRG 3 cut(s) 1131, 1175, 1302
EcoNI CCTNNNNNAGG 1 cut(s) 544
EcoRII CCWGG 1 cut(s) 300
EcoRV GATATC 1 cut(s) 786
EcoT14I CCWWGG 1 cut(s) 151
EcoT22I ATGCAT 2 cut(s) 780, 1597
ErhI CCWWGG 1 cut(s) 151
Esp3I CGTCTC 1 cut(s) 302
FaeI CATG 5 cut(s) 39, 46, 142, 752, 987
FaqI GGGAC 1 cut(s) 734
FatI CATG 5 cut(s) 35, 42, 138, 748, 983
FauI CCCGC 1 cut(s) 276
FokI GGATG 5 cut(s) 498, 518, 836, 1474, 1622
FspBI CTAG 5 cut(s) 701, 989, 1100, 1110, 1244
GlaI GCGC 7 cut(s) 191, 368, 580, 729, 889, 939, 1552
GsaI CCCAGC 1 cut(s) 1473
GsuI CTGGAG 2 cut(s) 67, 284
HaeII RGCGCY 1 cut(s) 891
HaeIII GGCC 3 cut(s) 5, 34, 1474
HapII CCGG 6 cut(s) 200, 232, 285, 381, 477, 1475
HhaI GCGC 7 cut(s) 192, 369, 581, 730, 890, 940, 1553
Hin1II CATG 5 cut(s) 39, 46, 142, 752, 987
Hin6I GCGC 7 cut(s) 190, 367, 579, 728, 888, 938, 1551
HinP1I GCGC 7 cut(s) 190, 367, 579, 728, 888, 938, 1551
HindIII AAGCTT 1 cut(s) 803
HinfI GANTC 5 cut(s) 158, 445, 658, 1145, 1305
HpaII CCGG 6 cut(s) 200, 232, 285, 381, 477, 1475
HphI GGTGA 6 cut(s) 155, 204, 1022, 1154, 1373, 1630
Hpy166II GTNNAC 4 cut(s) 131, 569, 1212, 1638
Hpy188I TCNGA 4 cut(s) 253, 409, 625, 783
Hpy188III TCNNGA 7 cut(s) 50, 227, 285, 503, 1163, 1624, 1667
Hpy8I GTNNAC 4 cut(s) 131, 569, 1212, 1638
HpyAV CCTTC 7 cut(s) 225, 371, 526, 683, 831, 1001, 1577
HpyCH4III ACNGT 4 cut(s) 79, 181, 1083, 1262
HpyCH4IV ACGT 2 cut(s) 295, 863
HpyF3I CTNAG 4 cut(s) 120, 236, 663, 1632
HpySE526I ACGT 2 cut(s) 295, 863
Hsp92II CATG 5 cut(s) 39, 46, 142, 752, 987
HspAI GCGC 7 cut(s) 190, 367, 579, 728, 888, 938, 1551
Kpn2I TCCGGA 1 cut(s) 284
KroI GCCGGC 1 cut(s) 476
KroNI GCCGGC 1 cut(s) 478
Kzo9I GATC 6 cut(s) 46, 409, 871, 973, 1159, 1557
LmnI GCTCC 1 cut(s) 245
Lsp1109I GCAGC 8 cut(s) 7, 13, 29, 35, 68, 81, 328, 957
LweI GCATC 9 cut(s) 81, 377, 520, 592, 748, 765, 787, 1140, 1604
MaeI CTAG 5 cut(s) 701, 989, 1100, 1110, 1244
MaeII ACGT 2 cut(s) 295, 863
MaeIII GTNAC 3 cut(s) 641, 1179, 1256
MalI GATC 6 cut(s) 48, 411, 873, 975, 1161, 1559
MboI GATC 6 cut(s) 46, 409, 871, 973, 1159, 1557
MfeI CAATTG 1 cut(s) 753
MflI RGATCY 3 cut(s) 46, 871, 1159
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 8 cut(s) 288, 321, 356, 739, 753, 1295, 1325, 1431
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 1314
MmeI TCCRAC 2 cut(s) 1333, 1433
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 2 cut(s) 780, 1597
MroI TCCGGA 1 cut(s) 284
MroNI GCCGGC 1 cut(s) 476
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 2 cut(s) 625, 1317
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 20, 26
MspI CCGG 6 cut(s) 200, 232, 285, 381, 477, 1475
MspR9I CCNGG 1 cut(s) 302
MunI CAATTG 1 cut(s) 753
MvaI CCWGG 1 cut(s) 302
MvnI CGCG 6 cut(s) 190, 367, 579, 938, 940, 1553
NaeI GCCGGC 1 cut(s) 478
NdeII GATC 6 cut(s) 46, 409, 871, 973, 1159, 1557
NgoMIV GCCGGC 1 cut(s) 476
NheI GCTAGC 2 cut(s) 700, 988
NlaIII CATG 5 cut(s) 39, 46, 142, 752, 987
NlaIV GGNNCC 6 cut(s) 48, 178, 207, 306, 551, 1129
NmeAIII GCCGAG 3 cut(s) 10, 893, 1527
NmuCI GTSAC 1 cut(s) 1256
NsiI ATGCAT 2 cut(s) 780, 1597
NspI RCATGY 1 cut(s) 752
NspV TTCGAA 1 cut(s) 808
PaeR7I CTCGAG 2 cut(s) 1175, 1302
PdiI GCCGGC 1 cut(s) 478
PfeI GAWTC 4 cut(s) 158, 445, 658, 1145
PflFI GACNNNGTC 1 cut(s) 1262
PflMI CCANNNNNTGG 2 cut(s) 717, 984
PleI GAGTC 1 cut(s) 1313
PpsI GAGTC 1 cut(s) 1313
Psp6I CCWGG 1 cut(s) 300
PspFI CCCAGC 1 cut(s) 1469
PspGI CCWGG 1 cut(s) 300
PspN4I GGNNCC 6 cut(s) 48, 178, 207, 306, 551, 1129
PspPI GGNCC 3 cut(s) 229, 1198, 1472
PspXI VCTCGAGB 2 cut(s) 1175, 1302
PsrI GAACNNNNNNTAC 2 cut(s) 1164, 1196
PstI CTGCAG 2 cut(s) 25, 493
PstNI CAGNNNCTG 1 cut(s) 965
PsuI RGATCY 3 cut(s) 46, 871, 1159
PsyI GACNNNGTC 1 cut(s) 1262
PvuII CAGCTG 2 cut(s) 20, 26
RsaI GTAC 3 cut(s) 524, 933, 1221
RsaNI GTAC 3 cut(s) 523, 932, 1220
RseI CAYNNNNRTG 2 cut(s) 625, 1317
Sau3AI GATC 6 cut(s) 46, 409, 871, 973, 1159, 1557
Sau96I GGNCC 3 cut(s) 229, 1198, 1472
SchI GAGTC 1 cut(s) 1314
ScrFI CCNGG 1 cut(s) 302
SfaNI GCATC 9 cut(s) 81, 377, 520, 592, 748, 765, 787, 1140, 1604
SfcI CTRYAG 3 cut(s) 21, 489, 558
Sfr274I CTCGAG 2 cut(s) 1175, 1302
SfuI TTCGAA 1 cut(s) 808
SinI GGWCC 2 cut(s) 229, 1198
SlaI CTCGAG 2 cut(s) 1175, 1302
SmiMI CAYNNNNRTG 2 cut(s) 625, 1317
SmlI CTYRAG 5 cut(s) 12, 1175, 1205, 1302, 1622
SmoI CTYRAG 5 cut(s) 12, 1175, 1205, 1302, 1622
Sse9I AATT 8 cut(s) 288, 321, 356, 739, 753, 1295, 1325, 1431
SsiI CCGC 8 cut(s) 60, 203, 243, 269, 365, 1040, 1436, 1511
SspMI CTAG 5 cut(s) 701, 989, 1100, 1110, 1244
StyD4I CCNGG 1 cut(s) 300
StyI CCWWGG 1 cut(s) 151
TaaI ACNGT 4 cut(s) 79, 181, 1083, 1262
TaiI ACGT 2 cut(s) 298, 866
TaqI TCGA 9 cut(s) 333, 600, 808, 824, 1148, 1176, 1303, 1452, 1617
TaqII GACCGA 1 cut(s) 1470
TasI AATT 8 cut(s) 288, 321, 356, 739, 753, 1295, 1325, 1431
TauI GCSGC 2 cut(s) 62, 1042
TfiI GAWTC 4 cut(s) 158, 445, 658, 1145
TscAI CASTG 1 cut(s) 1267
TseFI GTSAC 1 cut(s) 1256
TseI GCWGC 8 cut(s) 17, 20, 23, 26, 56, 69, 316, 945
Tsp45I GTSAC 1 cut(s) 1256
TspDTI ATGAA 5 cut(s) 31, 89, 155, 207, 1626
TspGWI ACGGA 3 cut(s) 264, 584, 1280
TspRI CASTG 1 cut(s) 1267
Tth111I GACNNNGTC 1 cut(s) 1262
Van91I CCANNNNNTGG 2 cut(s) 717, 984
VpaK11BI GGWCC 2 cut(s) 229, 1198
XagI CCTNNNNNAGG 1 cut(s) 544
XapI RAATTY 2 cut(s) 739, 1295
XceI RCATGY 1 cut(s) 752
XhoI CTCGAG 2 cut(s) 1175, 1302
XspI CTAG 5 cut(s) 701, 989, 1100, 1110, 1244
Zsp2I ATGCAT 2 cut(s) 780, 1597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.