RLG00000005245

Isochorismate synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0009008
Physical Location & Seq
Forward (+)
66200217 .. 66203820
3604 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005245

Sequence Viewer

Length: 1716 bp
ATGGCTGCTGGTTCTGCTGCTGCCGTTGGGCGGCGCTGCCTCGCATGCTTCATGGACCCTGAAGCAGCCAAATGCAGCTCCACTATTTCCAGCAGCAGACAAACCATTCATTTCCCCAACCATAGGAACAGTCACCAACGTTTTTGCTCGCTTTCGATGAATGGTTGCCAAGGAGACCACCCTCAGCTCCCTTTAGGTACAGTCGAAACGCGTTCATTTCCGGCAGTTCCATCACCGTCATTGGCCGTGGAGACACTCAGCTCCGCCCTTTCTCAGTTGAAAGCAAACCCACCTCCTTACACCTCTGGCATTATTCGTCTCCAAGTACCAATCCAGCAGAAAATTGAAGCAATTGACTGGCTGTATGCCCAAAATAAACTCCTTCCTCGCTGTTACTTCTCCGATAGAGCTCAAACCGGCAATCCTAAGCTTTCCATTGATTGCGCCAATGGGAATGGGAATGGTAATGGCCAGAGTCTGCAGAAGCATAACAAGTTGGTCAGTGTTGCTGGTGTGGGATCTGCAGTATTCTTCCAGGATCTGCATCCATTCTCATACAGGCATTGGAAGTCCTTAAAGAGGTTCCTGTCTACAGAATGTCCTCTAATTCGGGCATATGGGGCAATCCGGTTTGATGCAAGAGCCAATATATCATCCGAATGGCAGGCCTTTGGTTACTTCTATTTTATGGTCCCTCAGGTTGAGTTTGACGAGCTTGAAGATAGTTCAATTCTAGCAACAAATATTGCATGGGACAACTCCCTCTCGTGGACTTTGGGAAATGCCATTGATGAACTTAAAGCCATAATGCACCAGATATCTTCCATTGTTTTAAAGCTACAAAAACAAGTTCGTTCGATGCTAATGTTAAGCAATAGTCACATCCCAAGTAAGAAATATTGGGATCTTGCTGTTAATAGAGCTTTGCAGAAAATAAGAAGTAATTCTGCACTTATTAAGGTTGTACTCGCACGTAGCAGCAGAGTAATAACAGCTACTGATATTGATCCTATTGCATGGTTAGCTTGCTTACAGGTTGAAGGCAAAAATGCTTATCAGTTTTGTCTCCAGCCACCTGATGCACCAGCATTTATTGGAAACACGCCAGAGCAACTGTTTCACAGAACATGGCTTGACATCAATAGTGAAGCTCTGGCTGGAACCCGTGCCAGAGGTGAATCGCTGTCTGAAGATCTTCAAATAGAACTTGACCTGCTTTCCAGTCCTAAGGACCACCTTGAGTTTACTATTGTACGAGAAAGCATTAGAAAAAAATTAGAGAATGTATGCAACAGTGTAGTAGTTGAACCAAAGAAAGAAATCCGAAAACTCCCTAGAGTCCAACATTTATATGCCAAATTGTCTGGCAGGTTAAGAAGTGAAGATGATGAGTTTGAGATACTGTCTTCTCTTCACCCTAGTCCAGCCGTTTGTGGATTTCCAACAGAGGAGGCACGAGTTCTTATTGCAGAAACAGAAGTATTTGACCGAGGGATGTATGCTGGTCCAGTTGGTTGGTTTGGAGGAGGAGAGAGTGAGTTTGCTGTTGGCATTAGGTCAGCCTTAGTGGAGAAGCGTCTCGGTGCATTGATCTATGCTGGAACAGGAGTTGTGGAAGGAAGCAATGCATCATCAGAATGGGATGAACTGGAACTTAAAATTTCTCAGTTCACCAAGTTACTCAAACTTGAAGTGCCTTTAGAGATCAAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002376 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0006082 GO:0006575 GO:0006725 GO:0006732 GO:0006760 GO:0006766 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0008483 GO:0008909 GO:0009058 GO:0009108 GO:0009110 GO:0009266 GO:0009396 GO:0009409 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009627 GO:0009628 GO:0009696 GO:0009697 GO:0009814 GO:0009987 GO:0010118 GO:0010817 GO:0016020 GO:0016053 GO:0016740 GO:0016769 GO:0016853 GO:0016866 GO:0016999 GO:0017000 GO:0017144 GO:0018130 GO:0018958 GO:0019438 GO:0019752 GO:0019867 GO:0031090 GO:0031347 GO:0031348 GO:0031966 GO:0031967 GO:0031968 GO:0031975 GO:0032787 GO:0034641 GO:0042180 GO:0042181 GO:0042372 GO:0042374 GO:0042398 GO:0042445 GO:0042446 GO:0042537 GO:0042558 GO:0042559 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046189 GO:0046394 GO:0046483 GO:0046653 GO:0046654 GO:0046820 GO:0048519 GO:0048583 GO:0048585 GO:0050486 GO:0050789 GO:0050832 GO:0050896 GO:0051186 GO:0051188 GO:0051704 GO:0051707 GO:0065007 GO:0065008 GO:0071704 GO:0072330 GO:0080134 GO:0098542 GO:0098588 GO:0098805 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901615 GO:1901617 GO:1901661 GO:1901663
KEGG Pathways
Metabolic & Signaling
ko00130 ko01053 ko01100 ko01110 ko01130 ko04020 ko04022 ko04216 ko04217 ko04218 ko04621 ko04979 ko05012 ko05016 ko05166 map00130 map01053 map01100 map01110 map01130 map04020 map04022 map04216 map04217 map04218 map04621 map04979 map05012 map05016 map05166
Pfam Domains
Protein Families
Chorismate_bind Porin_3

Protein Analysis

572

Amino Acids

63.29

Weight (kDa)

7.57

Isoelectric Point (pI)

50.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Chorismate_bind PF00425 298 - 553 6.5e-64 chorismate binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1221, 1359
AccI GTMKAC 1 cut(s) 590
AccII CGCG 1 cut(s) 211
AciI CCGC 2 cut(s) 31, 264
AclI AACGTT 1 cut(s) 139
AclWI GGATC 4 cut(s) 526, 546, 912, 1001
AcoI YGGCCR 2 cut(s) 243, 469
AcsI RAATTY 1 cut(s) 1659
AcuI CTGAAG 2 cut(s) 81, 1209
AfaI GTAC 4 cut(s) 199, 327, 966, 1254
AfiI CCNNNNNNNGG 4 cut(s) 30, 123, 579, 768
AflIII ACRYGT 1 cut(s) 209
AgsI TTSAA 8 cut(s) 280, 347, 719, 729, 1040, 1199, 1307, 1691
AjnI CCWGG 1 cut(s) 534
Alw21I GWGCWC 1 cut(s) 412
Alw26I GTCTC 5 cut(s) 168, 245, 323, 1070, 1583
AlwI GGATC 4 cut(s) 526, 546, 912, 1001
AlwNI CAGNNNCTG 3 cut(s) 478, 541, 998
AoxI GGCC 3 cut(s) 243, 469, 666
ApeKI GCWGC 8 cut(s) 5, 17, 20, 36, 65, 75, 93, 978
ApoI RAATTY 1 cut(s) 1659
Asp700I GAANNNNTTC 2 cut(s) 943, 1194
AspLEI GCGC 2 cut(s) 36, 446
AspS9I GGNCC 4 cut(s) 55, 691, 1231, 1505
AsuHPI GGTGA 5 cut(s) 125, 225, 1187, 1406, 1663
AvaII GGWCC 4 cut(s) 55, 691, 1231, 1505
AxyI CCTNAGG 2 cut(s) 696, 1227
BalI TGGCCA 1 cut(s) 471
BanII GRGCYC 1 cut(s) 412
BauI CACGAG 2 cut(s) 766, 1455
BbsI GAAGAC 1 cut(s) 1398
Bbv12I GWGCWC 1 cut(s) 412
BbvCI CCTCAGC 1 cut(s) 183
BbvI GCAGC 7 cut(s) 4, 7, 23, 77, 87, 105, 990
BccI CCATC 1 cut(s) 238
BceAI ACGGC 3 cut(s) 8, 230, 1412
BciT130I CCWGG 1 cut(s) 536
BcoDI GTCTC 5 cut(s) 168, 245, 323, 1070, 1583
BfaI CTAG 3 cut(s) 734, 1335, 1419
BfmI CTRYAG 3 cut(s) 479, 522, 591
BfoI RGCGCY 1 cut(s) 37
BfuAI ACCTGC 2 cut(s) 1221, 1359
BglII AGATCT 1 cut(s) 1192
BisI GCNGC 9 cut(s) 6, 18, 21, 32, 37, 66, 76, 94, 979
BlsI GCNGC 9 cut(s) 7, 19, 22, 33, 38, 67, 77, 95, 980
Bme1390I CCNGG 1 cut(s) 536
Bme18I GGWCC 4 cut(s) 55, 691, 1231, 1505
BmgT120I GGNCC 4 cut(s) 55, 691, 1231, 1505
BmiI GGNNCC 4 cut(s) 57, 584, 693, 1162
BmrFI CCNGG 1 cut(s) 536
BmsI GCATC 5 cut(s) 553, 625, 849, 1069, 1637
BpiI GAAGAC 1 cut(s) 1398
BpmI CTGGAG 1 cut(s) 1052
Bpu10I CCTNAGC 2 cut(s) 183, 426
BpuEI CTTGAG 1 cut(s) 1259
BsaAI YACGTR 1 cut(s) 974
BsaBI GATNNNNATC 2 cut(s) 543, 1005
BsaI GGTCTC 1 cut(s) 168
BsaJI CCNNGG 3 cut(s) 169, 246, 1489
BsaWI WCCGGW 1 cut(s) 627
Bsc4I CCNNNNNNNGG 4 cut(s) 30, 123, 579, 768
Bse118I RCCGGY 1 cut(s) 416
Bse1I ACTGG 4 cut(s) 362, 1221, 1508, 1653
Bse21I CCTNAGG 2 cut(s) 696, 1227
Bse3DI GCAATG 1 cut(s) 1630
Bse8I GATNNNNATC 2 cut(s) 543, 1005
BseBI CCWGG 1 cut(s) 536
BseDI CCNNGG 3 cut(s) 169, 246, 1489
BseGI GGATG 5 cut(s) 544, 653, 882, 1500, 1648
BseJI GATNNNNATC 2 cut(s) 543, 1005
BseLI CCNNNNNNNGG 4 cut(s) 30, 123, 579, 768
BseMI GCAATG 1 cut(s) 1630
BseMII CTCAG 5 cut(s) 197, 271, 287, 710, 1679
BseNI ACTGG 4 cut(s) 362, 1221, 1508, 1653
BseRI GAGGAG 3 cut(s) 1463, 1539, 1542
BseXI GCAGC 7 cut(s) 4, 7, 23, 77, 87, 105, 990
BsgI GTGCAG 1 cut(s) 933
Bsh1236I CGCG 1 cut(s) 211
BshFI GGCC 3 cut(s) 245, 471, 668
BsiHKAI GWGCWC 1 cut(s) 412
BsiSI CCGG 3 cut(s) 221, 417, 628
BslFI GGGAC 2 cut(s) 677, 767
BslI CCNNNNNNNGG 4 cut(s) 30, 123, 579, 768
BsmAI GTCTC 5 cut(s) 168, 245, 323, 1070, 1583
BsmBI CGTCTC 2 cut(s) 323, 1583
BsmFI GGGAC 2 cut(s) 677, 767
BsnI GGCC 3 cut(s) 245, 471, 668
Bso31I GGTCTC 1 cut(s) 168
Bsp1286I GDGCHC 1 cut(s) 412
Bsp143I GATC 7 cut(s) 518, 538, 904, 1006, 1192, 1590, 1704
BspACI CCGC 2 cut(s) 31, 264
BspANI GGCC 3 cut(s) 245, 471, 668
BspCNI CTCAG 5 cut(s) 196, 270, 286, 709, 1678
BspFNI CGCG 1 cut(s) 211
BspLI GGNNCC 4 cut(s) 57, 584, 693, 1162
BspMAI CTGCAG 2 cut(s) 483, 526
BspMI ACCTGC 2 cut(s) 1221, 1359
BspPI GGATC 4 cut(s) 526, 546, 912, 1001
BspTNI GGTCTC 1 cut(s) 168
BsrDI GCAATG 1 cut(s) 1630
BsrFI RCCGGY 1 cut(s) 416
BsrI ACTGG 4 cut(s) 362, 1221, 1508, 1653
BssAI RCCGGY 1 cut(s) 416
BssECI CCNNGG 3 cut(s) 169, 246, 1489
BssMI GATC 7 cut(s) 518, 538, 904, 1006, 1192, 1590, 1704
BssSI CACGAG 2 cut(s) 766, 1455
BssT1I CCWWGG 1 cut(s) 169
Bst2BI CACGAG 2 cut(s) 766, 1455
Bst2UI CCWGG 1 cut(s) 536
Bst4CI ACNGT 6 cut(s) 131, 202, 237, 1116, 1295, 1404
Bst6I CTCTTC 1 cut(s) 1416
BstBAI YACGTR 1 cut(s) 974
BstC8I GCNNGC 4 cut(s) 46, 149, 666, 1027
BstDEI CTNAG 8 cut(s) 183, 257, 273, 426, 696, 1227, 1564, 1665
BstDSI CCRYGG 1 cut(s) 246
BstENI CCTNNNNNAGG 1 cut(s) 577
BstF5I GGATG 5 cut(s) 544, 653, 882, 1500, 1648
BstFNI CGCG 1 cut(s) 211
BstH2I RGCGCY 1 cut(s) 37
BstHHI GCGC 2 cut(s) 36, 446
BstKTI GATC 7 cut(s) 521, 541, 907, 1009, 1195, 1593, 1707
BstMAI GTCTC 5 cut(s) 168, 245, 323, 1070, 1583
BstMBI GATC 7 cut(s) 518, 538, 904, 1006, 1192, 1590, 1704
BstMWI GCNNNNNNNGC 4 cut(s) 14, 45, 620, 1022
BstNI CCWGG 1 cut(s) 536
BstNSI RCATGY 1 cut(s) 48
BstSCI CCNGG 1 cut(s) 534
BstSFI CTRYAG 3 cut(s) 479, 522, 591
BstUI CGCG 1 cut(s) 211
BstV1I GCAGC 7 cut(s) 4, 7, 23, 77, 87, 105, 990
BstV2I GAAGAC 1 cut(s) 1398
BstX2I RGATCY 4 cut(s) 518, 538, 904, 1192
BstXI CCANNNNNNTGG 1 cut(s) 1515
BstYI RGATCY 4 cut(s) 518, 538, 904, 1192
Bsu36I CCTNAGG 2 cut(s) 696, 1227
BsuRI GGCC 3 cut(s) 245, 471, 668
BtgI CCRYGG 1 cut(s) 246
BtsCI GGATG 5 cut(s) 544, 653, 882, 1500, 1648
BtsIMutI CAGTG 2 cut(s) 508, 1300
BveI ACCTGC 2 cut(s) 1221, 1359
Cac8I GCNNGC 4 cut(s) 46, 149, 666, 1027
CaiI CAGNNNCTG 3 cut(s) 478, 541, 998
CfoI GCGC 2 cut(s) 36, 446
Cfr10I RCCGGY 1 cut(s) 416
Cfr13I GGNCC 4 cut(s) 55, 691, 1231, 1505
CseI GACGC 1 cut(s) 1565
Csp6I GTAC 4 cut(s) 198, 326, 965, 1253
CviAII CATG 5 cut(s) 45, 52, 750, 1017, 1128
CviQI GTAC 4 cut(s) 198, 326, 965, 1253
DdeI CTNAG 8 cut(s) 183, 257, 273, 426, 696, 1227, 1564, 1665
DpnI GATC 7 cut(s) 520, 540, 906, 1008, 1194, 1592, 1706
DpnII GATC 7 cut(s) 518, 538, 904, 1006, 1192, 1590, 1704
DraI TTTAAA 1 cut(s) 834
EaeI YGGCCR 2 cut(s) 243, 469
Eam1104I CTCTTC 1 cut(s) 1416
EarI CTCTTC 1 cut(s) 1416
EciI GGCGGA 1 cut(s) 253
Ecl136II GAGCTC 1 cut(s) 410
Eco130I CCWWGG 1 cut(s) 169
Eco147I AGGCCT 1 cut(s) 668
Eco24I GRGCYC 1 cut(s) 412
Eco31I GGTCTC 1 cut(s) 168
Eco32I GATATC 1 cut(s) 819
Eco47I GGWCC 4 cut(s) 55, 691, 1231, 1505
Eco53kI GAGCTC 1 cut(s) 410
Eco57I CTGAAG 2 cut(s) 81, 1209
Eco81I CCTNAGG 2 cut(s) 696, 1227
EcoICRI GAGCTC 1 cut(s) 410
EcoNI CCTNNNNNAGG 1 cut(s) 577
EcoRII CCWGG 1 cut(s) 534
EcoRV GATATC 1 cut(s) 819
EcoT14I CCWWGG 1 cut(s) 169
EcoT22I ATGCAT 1 cut(s) 1630
EcoT38I GRGCYC 1 cut(s) 412
ErhI CCWWGG 1 cut(s) 169
Esp3I CGTCTC 2 cut(s) 323, 1583
FaeI CATG 5 cut(s) 48, 55, 753, 1020, 1131
FaqI GGGAC 2 cut(s) 677, 767
FatI CATG 5 cut(s) 44, 51, 749, 1016, 1127
FauNDI CATATG 1 cut(s) 616
FblI GTMKAC 1 cut(s) 590
Fnu4HI GCNGC 9 cut(s) 6, 18, 21, 32, 37, 66, 76, 94, 979
FokI GGATG 5 cut(s) 531, 640, 869, 1507, 1655
FriOI GRGCYC 1 cut(s) 412
Fsp4HI GCNGC 9 cut(s) 6, 18, 21, 32, 37, 66, 76, 94, 979
FspBI CTAG 3 cut(s) 734, 1335, 1419
GlaI GCGC 2 cut(s) 35, 445
GluI GCNGC 9 cut(s) 6, 18, 21, 32, 37, 66, 76, 94, 979
GsuI CTGGAG 1 cut(s) 1052
HaeII RGCGCY 1 cut(s) 37
HaeIII GGCC 3 cut(s) 245, 471, 668
HapII CCGG 3 cut(s) 221, 417, 628
HgaI GACGC 1 cut(s) 1565
HhaI GCGC 2 cut(s) 36, 446
Hin1II CATG 5 cut(s) 48, 55, 753, 1020, 1131
Hin6I GCGC 2 cut(s) 34, 444
HinP1I GCGC 2 cut(s) 34, 444
HindIII AAGCTT 1 cut(s) 428
HinfI GANTC 3 cut(s) 475, 1178, 1338
HpaII CCGG 3 cut(s) 221, 417, 628
HphI GGTGA 5 cut(s) 125, 225, 1187, 1406, 1663
Hpy166II GTNNAC 4 cut(s) 591, 771, 1245, 1671
Hpy188I TCNGA 5 cut(s) 403, 658, 1189, 1325, 1636
Hpy8I GTNNAC 4 cut(s) 591, 771, 1245, 1671
HpyAV CCTTC 3 cut(s) 392, 1034, 1610
HpyCH4III ACNGT 6 cut(s) 131, 202, 237, 1116, 1295, 1404
HpyCH4IV ACGT 2 cut(s) 139, 973
HpyF10VI GCNNNNNNNGC 4 cut(s) 14, 45, 620, 1022
HpyF3I CTNAG 8 cut(s) 183, 257, 273, 426, 696, 1227, 1564, 1665
HpySE526I ACGT 2 cut(s) 139, 973
Hsp92II CATG 5 cut(s) 48, 55, 753, 1020, 1131
HspAI GCGC 2 cut(s) 34, 444
Kzo9I GATC 7 cut(s) 518, 538, 904, 1006, 1192, 1590, 1704
LmnI GCTCC 3 cut(s) 83, 192, 266
Lsp1109I GCAGC 7 cut(s) 4, 7, 23, 77, 87, 105, 990
LweI GCATC 5 cut(s) 553, 625, 849, 1069, 1637
MaeI CTAG 3 cut(s) 734, 1335, 1419
MaeII ACGT 2 cut(s) 139, 973
MaeIII GTNAC 5 cut(s) 131, 392, 674, 878, 1677
MalI GATC 7 cut(s) 520, 540, 906, 1008, 1194, 1592, 1706
MboI GATC 7 cut(s) 518, 538, 904, 1006, 1192, 1590, 1704
MboII GAAGA 8 cut(s) 523, 731, 813, 1187, 1202, 1394, 1398, 1403
MfeI CAATTG 1 cut(s) 351
MflI RGATCY 4 cut(s) 518, 538, 904, 1192
MhlI GDGCHC 1 cut(s) 412
MlsI TGGCCA 1 cut(s) 471
MluCI AATT 8 cut(s) 342, 351, 606, 729, 943, 1274, 1358, 1659
MluI ACGCGT 1 cut(s) 209
MluNI TGGCCA 1 cut(s) 471
MlyI GAGTC 2 cut(s) 484, 1347
MmeI TCCRAC 2 cut(s) 1366, 1466
Mox20I TGGCCA 1 cut(s) 471
Mph1103I ATGCAT 1 cut(s) 1630
MroXI GAANNNNTTC 2 cut(s) 943, 1194
MscI TGGCCA 1 cut(s) 471
MseI TTAA 8 cut(s) 575, 798, 833, 869, 915, 957, 1373, 1656
MslI CAYNNNNRTG 3 cut(s) 658, 1350, 1636
Msp20I TGGCCA 1 cut(s) 471
MspI CCGG 3 cut(s) 221, 417, 628
MspR9I CCNGG 1 cut(s) 536
MunI CAATTG 1 cut(s) 351
MvaI CCWGG 1 cut(s) 536
MvnI CGCG 1 cut(s) 211
MwoI GCNNNNNNNGC 4 cut(s) 14, 45, 620, 1022
NdeI CATATG 1 cut(s) 616
NdeII GATC 7 cut(s) 518, 538, 904, 1006, 1192, 1590, 1704
NlaIII CATG 5 cut(s) 48, 55, 753, 1020, 1131
NlaIV GGNNCC 4 cut(s) 57, 584, 693, 1162
NmuCI GTSAC 2 cut(s) 131, 878
NsiI ATGCAT 1 cut(s) 1630
NspI RCATGY 1 cut(s) 48
PaeI GCATGC 1 cut(s) 48
PceI AGGCCT 1 cut(s) 668
PdmI GAANNNNTTC 2 cut(s) 943, 1194
PfeI GAWTC 1 cut(s) 1178
PfoI TCCNGGA 1 cut(s) 534
PkrI GCNGC 9 cut(s) 7, 19, 22, 33, 38, 67, 77, 95, 980
PleI GAGTC 2 cut(s) 483, 1346
PpsI GAGTC 2 cut(s) 483, 1346
Ppu21I YACGTR 1 cut(s) 974
Psp124BI GAGCTC 1 cut(s) 412
Psp1406I AACGTT 1 cut(s) 139
Psp6I CCWGG 1 cut(s) 534
PspGI CCWGG 1 cut(s) 534
PspN4I GGNNCC 4 cut(s) 57, 584, 693, 1162
PspPI GGNCC 4 cut(s) 55, 691, 1231, 1505
PstI CTGCAG 2 cut(s) 483, 526
PstNI CAGNNNCTG 3 cut(s) 478, 541, 998
PsuI RGATCY 4 cut(s) 518, 538, 904, 1192
RsaI GTAC 4 cut(s) 199, 327, 966, 1254
RsaNI GTAC 4 cut(s) 198, 326, 965, 1253
RseI CAYNNNNRTG 3 cut(s) 658, 1350, 1636
SacI GAGCTC 1 cut(s) 412
SaqAI TTAA 8 cut(s) 575, 798, 833, 869, 915, 957, 1373, 1656
SatI GCNGC 9 cut(s) 6, 18, 21, 32, 37, 66, 76, 94, 979
Sau3AI GATC 7 cut(s) 518, 538, 904, 1006, 1192, 1590, 1704
Sau96I GGNCC 4 cut(s) 55, 691, 1231, 1505
SchI GAGTC 2 cut(s) 484, 1347
ScrFI CCNGG 1 cut(s) 536
SduI GDGCHC 1 cut(s) 412
SfaNI GCATC 5 cut(s) 553, 625, 849, 1069, 1637
SfcI CTRYAG 3 cut(s) 479, 522, 591
SinI GGWCC 4 cut(s) 55, 691, 1231, 1505
SmiMI CAYNNNNRTG 3 cut(s) 658, 1350, 1636
SmlI CTYRAG 1 cut(s) 1238
SmoI CTYRAG 1 cut(s) 1238
SphI GCATGC 1 cut(s) 48
Sse9I AATT 8 cut(s) 342, 351, 606, 729, 943, 1274, 1358, 1659
SseBI AGGCCT 1 cut(s) 668
SsiI CCGC 2 cut(s) 31, 264
SspI AATATT 2 cut(s) 745, 899
SspMI CTAG 3 cut(s) 734, 1335, 1419
SstI GAGCTC 1 cut(s) 412
StuI AGGCCT 1 cut(s) 668
StyD4I CCNGG 1 cut(s) 534
StyI CCWWGG 1 cut(s) 169
TaaI ACNGT 6 cut(s) 131, 202, 237, 1116, 1295, 1404
TaiI ACGT 2 cut(s) 142, 976
TaqI TCGA 3 cut(s) 155, 204, 857
TaqII GACCGA 1 cut(s) 1503
TasI AATT 8 cut(s) 342, 351, 606, 729, 943, 1274, 1358, 1659
TatI WGTACW 1 cut(s) 964
TauI GCSGC 1 cut(s) 34
TfiI GAWTC 1 cut(s) 1178
Tru1I TTAA 8 cut(s) 575, 798, 833, 869, 915, 957, 1373, 1656
Tru9I TTAA 8 cut(s) 575, 798, 833, 869, 915, 957, 1373, 1656
TscAI CASTG 2 cut(s) 508, 1300
TseFI GTSAC 2 cut(s) 131, 878
TseI GCWGC 8 cut(s) 5, 17, 20, 36, 65, 75, 93, 978
Tsp45I GTSAC 2 cut(s) 131, 878
TspDTI ATGAA 6 cut(s) 40, 98, 173, 204, 807, 1659
TspRI CASTG 2 cut(s) 508, 1300
VpaK11BI GGWCC 4 cut(s) 55, 691, 1231, 1505
XagI CCTNNNNNAGG 1 cut(s) 577
XapI RAATTY 1 cut(s) 1659
XceI RCATGY 1 cut(s) 48
XmiI GTMKAC 1 cut(s) 590
XmnI GAANNNNTTC 2 cut(s) 943, 1194
XspI CTAG 3 cut(s) 734, 1335, 1419
Zsp2I ATGCAT 1 cut(s) 1630
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.