MD14G1195500.v1.1

Isochorismate synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0009008
Physical Location & Seq
Reverse (-)
28603652 .. 28605745
2094 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1195500.v1.1.491

Sequence Viewer

Length: 789 bp
ATGGCCACTGCTGCAGCCGCTGCCGCTGCTCGGCCATGTTTAATGGATCCCGAAGCAGCCGTCAAATGCAGCATCACAGTTTCGAGCAGGTCTCAGTTGTGTTCACTTTCCATGAATGGTTGCCAAGGAGTCTCTCACCTCCCTTTAGGCACCGTTGAAACGCGGTCGTTTCTGGCGGTTCCAACACCTTCATTGGCTCTGGACCGGCTCAGCTCCGCCATTTCAGAACTGAATTCAAACCCGCCTCCGTTTTCCTCCGGAATTATACGTCTCCAGCAATGGGCTTGTGCCACGTGGTGCCAATCCAGCAGCAAATTGAAGCAGTCGTACGCCCAAGACCAATTCCTTCCACGCTGCTTCTTTTCCGGTAGAGGCCAAACCAGCAACTCTTCTAATCTTTACATCGATAGCACCAATGGGAATGACCAGCAAAAATCGAAGAACAATTTGGTCAGCGTTGCCGGCGTGGGGTCTGCAGTTTTCTTTCAGGATCTGCATCCCTTCTCGTACAGGCATTGGAAGTCCTTAAAGAGGTTCCTCTCTACAGAATGTCCGCTTATTCGCGCTTATGGGGCAATCCGTTTCGATGCAAGAGCCGACATATCCTCCGAATGGGAGGCATTTGGTTACTTTTATTTTATGGTTCCTCAGGTTGAGTTTGATGAGCTTGAAGAAGGTTCAATGCTAGCAACAACCATTGCTTGGGACAGCGCTCTTTCGTGGACTTTGGGAGATGCAGTTGATGCACTTAAAGCTACCTTGCACCAGATATCTTCGGTTGTTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002376 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0006082 GO:0006575 GO:0006725 GO:0006732 GO:0006760 GO:0006766 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0008483 GO:0008909 GO:0009058 GO:0009108 GO:0009110 GO:0009266 GO:0009396 GO:0009409 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009627 GO:0009628 GO:0009696 GO:0009697 GO:0009814 GO:0009987 GO:0010118 GO:0010817 GO:0016020 GO:0016053 GO:0016740 GO:0016769 GO:0016853 GO:0016866 GO:0016999 GO:0017000 GO:0017144 GO:0018130 GO:0018958 GO:0019438 GO:0019752 GO:0019867 GO:0031090 GO:0031347 GO:0031348 GO:0031966 GO:0031967 GO:0031968 GO:0031975 GO:0032787 GO:0034641 GO:0042180 GO:0042181 GO:0042372 GO:0042374 GO:0042398 GO:0042445 GO:0042446 GO:0042537 GO:0042558 GO:0042559 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046189 GO:0046394 GO:0046483 GO:0046653 GO:0046654 GO:0046820 GO:0048519 GO:0048583 GO:0048585 GO:0050486 GO:0050789 GO:0050832 GO:0050896 GO:0051186 GO:0051188 GO:0051704 GO:0051707 GO:0065007 GO:0065008 GO:0071704 GO:0072330 GO:0080134 GO:0098542 GO:0098588 GO:0098805 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901615 GO:1901617 GO:1901661 GO:1901663
KEGG Pathways
Metabolic & Signaling
ko00130 ko01053 ko01100 ko01110 ko01130 ko04020 ko04022 ko04216 ko04217 ko04218 ko04621 ko04979 ko05012 ko05016 ko05166 map00130 map01053 map01100 map01110 map01130 map04020 map04022 map04216 map04217 map04218 map04621 map04979 map05012 map05016 map05166
Pfam Domains
Protein Families
Chorismate_bind Porin_3

Protein Analysis

263

Amino Acids

28.56

Weight (kDa)

6.08

Isoelectric Point (pI)

41.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 78
AccB1I GGYRCC 2 cut(s) 149, 297
AccB7I CCANNNNNTGG 1 cut(s) 702
AccII CGCG 2 cut(s) 163, 564
AccIII TCCGGA 1 cut(s) 257
AciI CCGC 7 cut(s) 18, 24, 163, 176, 216, 242, 554
AclWI GGATC 3 cut(s) 41, 54, 498
AcoI YGGCCR 2 cut(s) 3, 32
AcsI RAATTY 1 cut(s) 232
AcvI CACGTG 1 cut(s) 294
AdeI CACNNNGTG 1 cut(s) 297
AfaI GTAC 2 cut(s) 329, 509
AfeI AGCGCT 1 cut(s) 712
AfiI CCNNNNNNNGG 6 cut(s) 30, 280, 468, 531, 612, 702
AgsI TTSAA 5 cut(s) 158, 237, 319, 671, 681
AjuI GAANNNNNNNTTGG 2 cut(s) 431, 463
AluBI AGCT 3 cut(s) 213, 667, 755
AluI AGCT 3 cut(s) 213, 667, 755
Alw26I GTCTC 3 cut(s) 96, 136, 275
AlwI GGATC 3 cut(s) 41, 54, 498
AlwNI CAGNNNCTG 2 cut(s) 20, 493
Aor13HI TCCGGA 1 cut(s) 257
Aor51HI AGCGCT 1 cut(s) 712
AoxI GGCC 3 cut(s) 3, 32, 373
ApeKI GCWGC 8 cut(s) 11, 14, 20, 26, 56, 69, 309, 354
ApoI RAATTY 1 cut(s) 232
ArsI GACNNNNNNTTYG 2 cut(s) 45, 77
AspLEI GCGC 2 cut(s) 566, 713
AspS9I GGNCC 1 cut(s) 202
AsuHPI GGTGA 1 cut(s) 128
AsuNHI GCTAGC 1 cut(s) 685
AvaII GGWCC 1 cut(s) 202
AxyI CCTNAGG 1 cut(s) 648
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 46
BanI GGYRCC 2 cut(s) 149, 297
BarI GAAGNNNNNNTAC 2 cut(s) 311, 343
BbrPI CACGTG 1 cut(s) 294
BbvI GCAGC 7 cut(s) 7, 13, 26, 68, 81, 321, 341
BceAI ACGGC 1 cut(s) 44
BcoDI GTCTC 3 cut(s) 96, 136, 275
BfaI CTAG 1 cut(s) 686
BfmI CTRYAG 3 cut(s) 12, 474, 543
BfoI RGCGCY 1 cut(s) 714
BfuAI ACCTGC 1 cut(s) 78
BlpI GCTNAGC 1 cut(s) 209
Bme18I GGWCC 1 cut(s) 202
BmgT120I GGNCC 1 cut(s) 202
BmiI GGNNCC 6 cut(s) 48, 151, 180, 299, 536, 645
BmsI GCATC 5 cut(s) 81, 505, 577, 724, 733
BmtI GCTAGC 1 cut(s) 689
BplI GAGNNNNNCTC 2 cut(s) 76, 108
BpmI CTGGAG 1 cut(s) 257
Bpu1102I GCTNAGC 1 cut(s) 209
Bsa29I ATCGAT 1 cut(s) 405
BsaAI YACGTR 1 cut(s) 294
BsaBI GATNNNNATC 1 cut(s) 495
BsaI GGTCTC 1 cut(s) 96
BsaJI CCNNGG 1 cut(s) 124
BsaWI WCCGGW 2 cut(s) 257, 365
BsaXI ACNNNNNCTCC 4 cut(s) 590, 620, 723, 753
Bsc4I CCNNNNNNNGG 6 cut(s) 30, 280, 468, 531, 612, 702
Bse118I RCCGGY 2 cut(s) 204, 461
Bse21I CCTNAGG 1 cut(s) 648
Bse3DI GCAATG 2 cut(s) 284, 696
Bse8I GATNNNNATC 1 cut(s) 495
BseAI TCCGGA 1 cut(s) 257
BseCI ATCGAT 1 cut(s) 405
BseDI CCNNGG 1 cut(s) 124
BseGI GGATG 1 cut(s) 496
BseJI GATNNNNATC 1 cut(s) 495
BseLI CCNNNNNNNGG 6 cut(s) 30, 280, 468, 531, 612, 702
BseMI GCAATG 2 cut(s) 284, 696
BseMII CTCAG 3 cut(s) 107, 223, 662
BseXI GCAGC 7 cut(s) 7, 13, 26, 68, 81, 321, 341
Bsh1236I CGCG 2 cut(s) 163, 564
Bsh1285I CGRYCG 1 cut(s) 167
BshFI GGCC 3 cut(s) 5, 34, 375
BshNI GGYRCC 2 cut(s) 149, 297
BshVI ATCGAT 1 cut(s) 405
BsiEI CGRYCG 1 cut(s) 167
BsiSI CCGG 4 cut(s) 205, 258, 366, 462
BsiWI CGTACG 1 cut(s) 327
BslFI GGGAC 1 cut(s) 719
BslI CCNNNNNNNGG 6 cut(s) 30, 280, 468, 531, 612, 702
BsmAI GTCTC 3 cut(s) 96, 136, 275
BsmBI CGTCTC 1 cut(s) 275
BsmFI GGGAC 1 cut(s) 719
BsnI GGCC 3 cut(s) 5, 34, 375
Bso31I GGTCTC 1 cut(s) 96
Bsp13I TCCGGA 1 cut(s) 257
Bsp143I GATC 2 cut(s) 46, 490
Bsp1720I GCTNAGC 1 cut(s) 209
BspACI CCGC 7 cut(s) 18, 24, 163, 176, 216, 242, 554
BspANI GGCC 3 cut(s) 5, 34, 375
BspCNI CTCAG 3 cut(s) 106, 222, 661
BspDI ATCGAT 1 cut(s) 405
BspEI TCCGGA 1 cut(s) 257
BspFNI CGCG 2 cut(s) 163, 564
BspLI GGNNCC 6 cut(s) 48, 151, 180, 299, 536, 645
BspMAI CTGCAG 2 cut(s) 16, 478
BspMI ACCTGC 1 cut(s) 78
BspOI GCTAGC 1 cut(s) 689
BspPI GGATC 3 cut(s) 41, 54, 498
BspT107I GGYRCC 2 cut(s) 149, 297
BspTNI GGTCTC 1 cut(s) 96
BsrDI GCAATG 2 cut(s) 284, 696
BsrFI RCCGGY 2 cut(s) 204, 461
BssAI RCCGGY 2 cut(s) 204, 461
BssECI CCNNGG 1 cut(s) 124
BssMI GATC 2 cut(s) 46, 490
BssT1I CCWWGG 1 cut(s) 124
Bst4CI ACNGT 2 cut(s) 79, 154
Bst6I CTCTTC 1 cut(s) 394
BstAPI GCANNNNNTGC 2 cut(s) 20, 743
BstBAI YACGTR 1 cut(s) 294
BstC8I GCNNGC 2 cut(s) 463, 687
BstDEI CTNAG 3 cut(s) 93, 209, 648
BstENI CCTNNNNNAGG 1 cut(s) 529
BstF5I GGATG 1 cut(s) 496
BstFNI CGCG 2 cut(s) 163, 564
BstH2I RGCGCY 1 cut(s) 714
BstHHI GCGC 2 cut(s) 566, 713
BstKTI GATC 2 cut(s) 49, 493
BstMAI GTCTC 3 cut(s) 96, 136, 275
BstMBI GATC 2 cut(s) 46, 490
BstMCI CGRYCG 1 cut(s) 167
BstSFI CTRYAG 3 cut(s) 12, 474, 543
BstUI CGCG 2 cut(s) 163, 564
BstV1I GCAGC 7 cut(s) 7, 13, 26, 68, 81, 321, 341
BstX2I RGATCY 2 cut(s) 46, 490
BstYI RGATCY 2 cut(s) 46, 490
Bsu15I ATCGAT 1 cut(s) 405
Bsu36I CCTNAGG 1 cut(s) 648
BsuRI GGCC 3 cut(s) 5, 34, 375
BsuTUI ATCGAT 1 cut(s) 405
BtsCI GGATG 1 cut(s) 496
BtsI GCAGTG 1 cut(s) 6
BtsIMutI CAGTG 1 cut(s) 6
BveI ACCTGC 1 cut(s) 78
Cac8I GCNNGC 2 cut(s) 463, 687
CaiI CAGNNNCTG 2 cut(s) 20, 493
CfoI GCGC 2 cut(s) 566, 713
Cfr10I RCCGGY 2 cut(s) 204, 461
Cfr13I GGNCC 1 cut(s) 202
ClaI ATCGAT 1 cut(s) 405
Csp6I GTAC 2 cut(s) 328, 508
CviAII CATG 2 cut(s) 36, 112
CviQI GTAC 2 cut(s) 328, 508
DdeI CTNAG 3 cut(s) 93, 209, 648
DpnI GATC 2 cut(s) 48, 492
DpnII GATC 2 cut(s) 46, 490
DraIII CACNNNGTG 1 cut(s) 297
EaeI YGGCCR 2 cut(s) 3, 32
Eam1104I CTCTTC 1 cut(s) 394
EarI CTCTTC 1 cut(s) 394
EciI GGCGGA 1 cut(s) 205
Eco130I CCWWGG 1 cut(s) 124
Eco31I GGTCTC 1 cut(s) 96
Eco32I GATATC 1 cut(s) 771
Eco47I GGWCC 1 cut(s) 202
Eco47III AGCGCT 1 cut(s) 712
Eco72I CACGTG 1 cut(s) 294
Eco81I CCTNAGG 1 cut(s) 648
EcoNI CCTNNNNNAGG 1 cut(s) 529
EcoRI GAATTC 1 cut(s) 232
EcoRV GATATC 1 cut(s) 771
EcoT14I CCWWGG 1 cut(s) 124
ErhI CCWWGG 1 cut(s) 124
Esp3I CGTCTC 1 cut(s) 275
FaeI CATG 2 cut(s) 39, 115
FaiI YATR 7 cut(s) 37, 113, 266, 570, 602, 641, 787
FaqI GGGAC 1 cut(s) 719
FatI CATG 2 cut(s) 35, 111
FauI CCCGC 1 cut(s) 249
FokI GGATG 1 cut(s) 483
FspBI CTAG 1 cut(s) 686
GlaI GCGC 2 cut(s) 565, 712
GsuI CTGGAG 1 cut(s) 257
HaeII RGCGCY 1 cut(s) 714
HaeIII GGCC 3 cut(s) 5, 34, 375
HapII CCGG 4 cut(s) 205, 258, 366, 462
HhaI GCGC 2 cut(s) 566, 713
Hin1II CATG 2 cut(s) 39, 115
Hin6I GCGC 2 cut(s) 564, 711
HinP1I GCGC 2 cut(s) 564, 711
HinfI GANTC 1 cut(s) 129
HpaII CCGG 4 cut(s) 205, 258, 366, 462
HphI GGTGA 1 cut(s) 128
Hpy166II GTNNAC 2 cut(s) 104, 723
Hpy188I TCNGA 2 cut(s) 226, 610
Hpy188III TCNNGA 4 cut(s) 50, 200, 258, 488
Hpy8I GTNNAC 2 cut(s) 104, 723
HpyAV CCTTC 4 cut(s) 198, 356, 511, 668
HpyCH4III ACNGT 2 cut(s) 79, 154
HpyCH4IV ACGT 2 cut(s) 268, 293
HpyCH4V TGCA 8 cut(s) 14, 69, 476, 496, 590, 737, 746, 763
HpyF3I CTNAG 3 cut(s) 93, 209, 648
HpySE526I ACGT 2 cut(s) 268, 293
Hsp92II CATG 2 cut(s) 39, 115
HspAI GCGC 2 cut(s) 564, 711
Kpn2I TCCGGA 1 cut(s) 257
KroI GCCGGC 1 cut(s) 461
KroNI GCCGGC 1 cut(s) 463
Kzo9I GATC 2 cut(s) 46, 490
LmnI GCTCC 1 cut(s) 218
Lsp1109I GCAGC 7 cut(s) 7, 13, 26, 68, 81, 321, 341
LweI GCATC 5 cut(s) 81, 505, 577, 724, 733
MaeI CTAG 1 cut(s) 686
MaeII ACGT 2 cut(s) 268, 293
MaeIII GTNAC 1 cut(s) 626
MalI GATC 2 cut(s) 48, 492
MboI GATC 2 cut(s) 46, 490
MboII GAAGA 4 cut(s) 381, 451, 683, 765
MflI RGATCY 2 cut(s) 46, 490
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 5 cut(s) 232, 261, 314, 341, 445
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 138
MmeI TCCRAC 1 cut(s) 206
MnlI CCTC 9 cut(s) 149, 255, 265, 365, 525, 548, 610, 616, 657
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 257
MroNI GCCGGC 1 cut(s) 461
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 41, 527, 750
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 20, 26
MspI CCGG 4 cut(s) 205, 258, 366, 462
MvnI CGCG 2 cut(s) 163, 564
NaeI GCCGGC 1 cut(s) 463
NdeII GATC 2 cut(s) 46, 490
NgoMIV GCCGGC 1 cut(s) 461
NheI GCTAGC 1 cut(s) 685
NlaIII CATG 2 cut(s) 39, 115
NlaIV GGNNCC 6 cut(s) 48, 151, 180, 299, 536, 645
NmeAIII GCCGAG 1 cut(s) 10
PdiI GCCGGC 1 cut(s) 463
Pfl23II CGTACG 1 cut(s) 327
PflMI CCANNNNNTGG 1 cut(s) 702
PleI GAGTC 1 cut(s) 137
PmaCI CACGTG 1 cut(s) 294
PmlI CACGTG 1 cut(s) 294
PpsI GAGTC 1 cut(s) 137
Ppu21I YACGTR 1 cut(s) 294
PspCI CACGTG 1 cut(s) 294
PspLI CGTACG 1 cut(s) 327
PspN4I GGNNCC 6 cut(s) 48, 151, 180, 299, 536, 645
PspPI GGNCC 1 cut(s) 202
PstI CTGCAG 2 cut(s) 16, 478
PstNI CAGNNNCTG 2 cut(s) 20, 493
PsuI RGATCY 2 cut(s) 46, 490
RsaI GTAC 2 cut(s) 329, 509
RsaNI GTAC 2 cut(s) 328, 508
SaqAI TTAA 3 cut(s) 41, 527, 750
Sau3AI GATC 2 cut(s) 46, 490
Sau96I GGNCC 1 cut(s) 202
SchI GAGTC 1 cut(s) 138
SfaNI GCATC 5 cut(s) 81, 505, 577, 724, 733
SfcI CTRYAG 3 cut(s) 12, 474, 543
SinI GGWCC 1 cut(s) 202
Sse9I AATT 5 cut(s) 232, 261, 314, 341, 445
SsiI CCGC 7 cut(s) 18, 24, 163, 176, 216, 242, 554
SspMI CTAG 1 cut(s) 686
StyI CCWWGG 1 cut(s) 124
TaaI ACNGT 2 cut(s) 79, 154
TaiI ACGT 2 cut(s) 271, 296
TaqI TCGA 4 cut(s) 83, 405, 437, 585
TasI AATT 5 cut(s) 232, 261, 314, 341, 445
TauI GCSGC 2 cut(s) 20, 26
Tru1I TTAA 3 cut(s) 41, 527, 750
Tru9I TTAA 3 cut(s) 41, 527, 750
TscAI CASTG 1 cut(s) 13
TseI GCWGC 8 cut(s) 11, 14, 20, 26, 56, 69, 309, 354
TspDTI ATGAA 2 cut(s) 128, 180
TspGWI ACGGA 2 cut(s) 237, 569
TspRI CASTG 1 cut(s) 13
Van91I CCANNNNNTGG 1 cut(s) 702
VpaK11BI GGWCC 1 cut(s) 202
XagI CCTNNNNNAGG 1 cut(s) 529
XapI RAATTY 1 cut(s) 232
XspI CTAG 1 cut(s) 686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.