Rh7AG036100

Isochorismate synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0009008
Physical Location & Seq
Reverse (-)
2472855 .. 2477069
4215 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG036100.1

Sequence Viewer

Length: 1302 bp
ATGTTGTCACAAAAAGTCTCCCTCTCATTTTATAAAAGCTTAGCCTATCATATATTTCCATCTCCCATTGGAAGCTCTCTCTTTGTTTCTCCCATGGCTGCTGGTTCTGCTGCTGCCGTTGGGCGGCGCTGCCTCGCATGCTTCATCGACCCTGAATCAGCCAAATGCAGCTCCTCTATTTCCAGCAGCAGCAGCAGCAGACAAACCATTCATTTCCCCAACCAAAGGAACAGTCACCAACGTTTTTGCTCGCTTTCGATGAATGGTTGCCAAGGAGACCACCCTCACCTCCCTTTAGGTACAGTCGAAACGCGTTCATTTCCGGCAGTTCCATCACCGTCATTGGCCGTGGAGACACTCAGCTCCGCCCTTTCTCAGTTGAAAGCAAACCCACCTGCTTACACCTCTGGCATTATTCGTCTCCAAGTACCAATCCAGCAGAAAATTGAAGCAATTGACTGGCTGTATGCCCAAAATAAACTCCTTCCTCGCTGTTACTTCTCCGATAGAGCTCAAACTGGCAGTCCTAAGCGTTCCATTGATTGCGCCAATGGGAATGGGAATGGTAATGGCCAGAGTCCAAAGAAGCATAACAAGTTGGTCAGTGTTGCTGGTGTGGGATCTGCAGTATTCTTCCAGGATCTGCATCCATTCTCCTACAGGCATTGGAAGTCCTTAAAGAGATTCCTGTCTACAGACTGTCCTCTAATTCGGGCATATGGGGCAATCCGGTTTGATGCAAGAGCCACTATATCATCCGAATGGGAGGCCTTTGGTTACTTCTATTTTATGGTCCCTCAGGTTGAGTTTGACGAGCTTGAAGATAGTTCAATACTAGCAACAACTATTGCATGGGACAACTCCCTCTCGTGGACTTTGGGAAATGCCATTGATGAACTTAAAGCCACAATGCACCAGATATCTTCCATTGTTTTAAAGCTACAAAAACAAGTTTGTTCGATGCTAATGTTAAGCAATAGTCACATCCCAAGTAAGAAATATTGGGATCTTGCTGTTAATAGAGCTTTGCAGAAAATAAGAAGTGATTCAGCACTTATTAAGGTTGTACTCGCACGTAGCAGCAGAGTAATAACAGCTACTGATATTGATCCTATTGCATGGTTAGCTTGCTTACAGGTTGAAGGCAAAAATGCTTATCAGTTTTGTCTCCAACCACCTGATGCACCAACATTTATTGGAAACACGAGCAATTGTTTCACAGAACATGGCTTGACATCAATAGTGAAGCTCTGGCTGGAACCCGTGCCAGAGGTAAATCGCTGTCTGAAGATCTTCAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002376 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0006082 GO:0006575 GO:0006725 GO:0006732 GO:0006760 GO:0006766 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0008483 GO:0008909 GO:0009058 GO:0009108 GO:0009110 GO:0009266 GO:0009396 GO:0009409 GO:0009507 GO:0009536 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009627 GO:0009628 GO:0009696 GO:0009697 GO:0009814 GO:0009987 GO:0010118 GO:0010817 GO:0016020 GO:0016053 GO:0016740 GO:0016769 GO:0016853 GO:0016866 GO:0016999 GO:0017000 GO:0017144 GO:0018130 GO:0018958 GO:0019438 GO:0019752 GO:0019867 GO:0031090 GO:0031347 GO:0031348 GO:0031966 GO:0031967 GO:0031968 GO:0031975 GO:0032787 GO:0034641 GO:0042180 GO:0042181 GO:0042372 GO:0042374 GO:0042398 GO:0042445 GO:0042446 GO:0042537 GO:0042558 GO:0042559 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043603 GO:0043604 GO:0044237 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0046189 GO:0046394 GO:0046483 GO:0046653 GO:0046654 GO:0046820 GO:0048519 GO:0048583 GO:0048585 GO:0050486 GO:0050789 GO:0050832 GO:0050896 GO:0051186 GO:0051188 GO:0051704 GO:0051707 GO:0065007 GO:0065008 GO:0071704 GO:0072330 GO:0080134 GO:0098542 GO:0098588 GO:0098805 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901615 GO:1901617 GO:1901661 GO:1901663
KEGG Pathways
Metabolic & Signaling
ko00130 ko01053 ko01100 ko01110 ko01130 ko04020 ko04022 ko04216 ko04217 ko04218 ko04621 ko04979 ko05012 ko05016 ko05166 map00130 map01053 map01100 map01110 map01130 map04020 map04022 map04216 map04217 map04218 map04621 map04979 map05012 map05016 map05166
Pfam Domains
Protein Families
Chorismate_bind Porin_3

Protein Analysis

433

Amino Acids

47.85

Weight (kDa)

9.1

Isoelectric Point (pI)

53.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 33
AarI CACCTGC 1 cut(s) 403
Acc36I ACCTGC 1 cut(s) 403
AccI GTMKAC 1 cut(s) 692
AccII CGCG 1 cut(s) 313
AciI CCGC 2 cut(s) 124, 366
AclI AACGTT 1 cut(s) 241
AclWI GGATC 4 cut(s) 628, 648, 1014, 1103
AcoI YGGCCR 2 cut(s) 345, 571
AcuI CTGAAG 1 cut(s) 1279
AfaI GTAC 3 cut(s) 301, 429, 1068
AfiI CCNNNNNNNGG 3 cut(s) 123, 225, 870
AflIII ACRYGT 1 cut(s) 311
AgsI TTSAA 5 cut(s) 382, 449, 821, 831, 1142
AjnI CCWGG 1 cut(s) 636
Alw21I GWGCWC 1 cut(s) 514
Alw26I GTCTC 5 cut(s) 22, 270, 347, 425, 1172
AlwI GGATC 4 cut(s) 628, 648, 1014, 1103
AlwNI CAGNNNCTG 2 cut(s) 643, 1100
AoxI GGCC 3 cut(s) 345, 571, 768
ArsI GACNNNNNNTTYG 4 cut(s) 217, 249, 508, 540
Asp700I GAANNNNTTC 2 cut(s) 1045, 1292
AspLEI GCGC 2 cut(s) 129, 548
AspS9I GGNCC 1 cut(s) 793
AsuHPI GGTGA 3 cut(s) 227, 278, 327
AvaII GGWCC 1 cut(s) 793
AxyI CCTNAGG 1 cut(s) 798
BalI TGGCCA 1 cut(s) 573
BanII GRGCYC 1 cut(s) 514
BauI CACGAG 2 cut(s) 868, 1204
Bbv12I GWGCWC 1 cut(s) 514
BccI CCATC 2 cut(s) 67, 340
BceAI ACGGC 2 cut(s) 101, 332
BciT130I CCWGG 1 cut(s) 638
BcoDI GTCTC 5 cut(s) 22, 270, 347, 425, 1172
BfaI CTAG 1 cut(s) 836
BfmI CTRYAG 3 cut(s) 624, 658, 693
BfoI RGCGCY 1 cut(s) 130
BfuAI ACCTGC 1 cut(s) 403
BglII AGATCT 1 cut(s) 1290
BlpI GCTNAGC 1 cut(s) 40
Bme1390I CCNGG 1 cut(s) 638
Bme18I GGWCC 1 cut(s) 793
BmgT120I GGNCC 1 cut(s) 793
BmiI GGNNCC 2 cut(s) 795, 1260
BmrFI CCNGG 1 cut(s) 638
BmsI GCATC 4 cut(s) 655, 727, 951, 1171
Bpu10I CCTNAGC 1 cut(s) 528
Bpu1102I GCTNAGC 1 cut(s) 40
BsaAI YACGTR 1 cut(s) 1076
BsaBI GATNNNNATC 2 cut(s) 645, 1107
BsaI GGTCTC 1 cut(s) 270
BsaJI CCNNGG 3 cut(s) 93, 271, 348
BsaWI WCCGGW 1 cut(s) 729
Bsc4I CCNNNNNNNGG 3 cut(s) 123, 225, 870
Bse1I ACTGG 2 cut(s) 464, 523
Bse21I CCTNAGG 1 cut(s) 798
Bse8I GATNNNNATC 2 cut(s) 645, 1107
BseBI CCWGG 1 cut(s) 638
BseDI CCNNGG 3 cut(s) 93, 271, 348
BseGI GGATG 3 cut(s) 646, 755, 984
BseJI GATNNNNATC 2 cut(s) 645, 1107
BseLI CCNNNNNNNGG 3 cut(s) 123, 225, 870
BseMII CTCAG 3 cut(s) 373, 389, 812
BseNI ACTGG 2 cut(s) 464, 523
BseRI GAGGAG 1 cut(s) 163
Bsh1236I CGCG 1 cut(s) 313
BshFI GGCC 3 cut(s) 347, 573, 770
BsiHKAI GWGCWC 1 cut(s) 514
BsiSI CCGG 2 cut(s) 323, 730
BslFI GGGAC 2 cut(s) 779, 869
BslI CCNNNNNNNGG 3 cut(s) 123, 225, 870
BsmAI GTCTC 5 cut(s) 22, 270, 347, 425, 1172
BsmBI CGTCTC 1 cut(s) 425
BsmFI GGGAC 2 cut(s) 779, 869
BsnI GGCC 3 cut(s) 347, 573, 770
Bso31I GGTCTC 1 cut(s) 270
Bsp1286I GDGCHC 1 cut(s) 514
Bsp143I GATC 5 cut(s) 620, 640, 1006, 1108, 1290
Bsp1720I GCTNAGC 1 cut(s) 40
Bsp19I CCATGG 1 cut(s) 93
BspACI CCGC 2 cut(s) 124, 366
BspANI GGCC 3 cut(s) 347, 573, 770
BspCNI CTCAG 3 cut(s) 372, 388, 811
BspFNI CGCG 1 cut(s) 313
BspLI GGNNCC 2 cut(s) 795, 1260
BspMAI CTGCAG 1 cut(s) 628
BspMI ACCTGC 1 cut(s) 403
BspPI GGATC 4 cut(s) 628, 648, 1014, 1103
BspTNI GGTCTC 1 cut(s) 270
BsrI ACTGG 2 cut(s) 464, 523
BssECI CCNNGG 3 cut(s) 93, 271, 348
BssMI GATC 5 cut(s) 620, 640, 1006, 1108, 1290
BssSI CACGAG 2 cut(s) 868, 1204
BssT1I CCWWGG 2 cut(s) 93, 271
Bst2BI CACGAG 2 cut(s) 868, 1204
Bst2UI CCWGG 1 cut(s) 638
Bst4CI ACNGT 4 cut(s) 233, 304, 339, 701
BstBAI YACGTR 1 cut(s) 1076
BstC8I GCNNGC 3 cut(s) 139, 251, 1129
BstDEI CTNAG 5 cut(s) 40, 359, 375, 528, 798
BstDSI CCRYGG 2 cut(s) 93, 348
BstF5I GGATG 3 cut(s) 646, 755, 984
BstFNI CGCG 1 cut(s) 313
BstH2I RGCGCY 1 cut(s) 130
BstHHI GCGC 2 cut(s) 129, 548
BstKTI GATC 5 cut(s) 623, 643, 1009, 1111, 1293
BstMAI GTCTC 5 cut(s) 22, 270, 347, 425, 1172
BstMBI GATC 5 cut(s) 620, 640, 1006, 1108, 1290
BstMWI GCNNNNNNNGC 6 cut(s) 107, 138, 192, 195, 722, 1124
BstNI CCWGG 1 cut(s) 638
BstNSI RCATGY 1 cut(s) 141
BstSCI CCNGG 1 cut(s) 636
BstSFI CTRYAG 3 cut(s) 624, 658, 693
BstUI CGCG 1 cut(s) 313
BstX2I RGATCY 4 cut(s) 620, 640, 1006, 1290
BstYI RGATCY 4 cut(s) 620, 640, 1006, 1290
Bsu36I CCTNAGG 1 cut(s) 798
BsuRI GGCC 3 cut(s) 347, 573, 770
BtgI CCRYGG 2 cut(s) 93, 348
BtsCI GGATG 3 cut(s) 646, 755, 984
BtsIMutI CAGTG 1 cut(s) 610
BveI ACCTGC 1 cut(s) 403
Cac8I GCNNGC 3 cut(s) 139, 251, 1129
CaiI CAGNNNCTG 2 cut(s) 643, 1100
CfoI GCGC 2 cut(s) 129, 548
Cfr13I GGNCC 1 cut(s) 793
Csp6I GTAC 3 cut(s) 300, 428, 1067
CviAII CATG 5 cut(s) 94, 138, 852, 1119, 1226
CviQI GTAC 3 cut(s) 300, 428, 1067
DdeI CTNAG 5 cut(s) 40, 359, 375, 528, 798
DpnI GATC 5 cut(s) 622, 642, 1008, 1110, 1292
DpnII GATC 5 cut(s) 620, 640, 1006, 1108, 1290
DraI TTTAAA 1 cut(s) 936
EaeI YGGCCR 2 cut(s) 345, 571
EciI GGCGGA 1 cut(s) 355
Ecl136II GAGCTC 1 cut(s) 512
Eco130I CCWWGG 2 cut(s) 93, 271
Eco147I AGGCCT 1 cut(s) 770
Eco24I GRGCYC 1 cut(s) 514
Eco31I GGTCTC 1 cut(s) 270
Eco32I GATATC 1 cut(s) 921
Eco47I GGWCC 1 cut(s) 793
Eco53kI GAGCTC 1 cut(s) 512
Eco57I CTGAAG 1 cut(s) 1279
Eco81I CCTNAGG 1 cut(s) 798
EcoICRI GAGCTC 1 cut(s) 512
EcoRII CCWGG 1 cut(s) 636
EcoRV GATATC 1 cut(s) 921
EcoT14I CCWWGG 2 cut(s) 93, 271
EcoT38I GRGCYC 1 cut(s) 514
ErhI CCWWGG 2 cut(s) 93, 271
Esp3I CGTCTC 1 cut(s) 425
FaeI CATG 5 cut(s) 97, 141, 855, 1122, 1229
FaqI GGGAC 2 cut(s) 779, 869
FatI CATG 5 cut(s) 93, 137, 851, 1118, 1225
FauNDI CATATG 1 cut(s) 718
FblI GTMKAC 1 cut(s) 692
FokI GGATG 3 cut(s) 633, 742, 971
FriOI GRGCYC 1 cut(s) 514
FspBI CTAG 1 cut(s) 836
GlaI GCGC 2 cut(s) 128, 547
HaeII RGCGCY 1 cut(s) 130
HaeIII GGCC 3 cut(s) 347, 573, 770
HapII CCGG 2 cut(s) 323, 730
HhaI GCGC 2 cut(s) 129, 548
Hin1II CATG 5 cut(s) 97, 141, 855, 1122, 1229
Hin6I GCGC 2 cut(s) 127, 546
HinP1I GCGC 2 cut(s) 127, 546
HindIII AAGCTT 1 cut(s) 37
HinfI GANTC 4 cut(s) 155, 577, 684, 1046
HpaII CCGG 2 cut(s) 323, 730
HphI GGTGA 3 cut(s) 227, 278, 327
Hpy166II GTNNAC 2 cut(s) 693, 873
Hpy188I TCNGA 4 cut(s) 505, 760, 1287, 1298
Hpy8I GTNNAC 2 cut(s) 693, 873
HpyAV CCTTC 2 cut(s) 494, 1136
HpyCH4III ACNGT 4 cut(s) 233, 304, 339, 701
HpyCH4IV ACGT 2 cut(s) 241, 1075
HpyCH4V TGCA 9 cut(s) 168, 626, 646, 740, 851, 913, 1030, 1118, 1184
HpyF10VI GCNNNNNNNGC 6 cut(s) 107, 138, 192, 195, 722, 1124
HpyF3I CTNAG 5 cut(s) 40, 359, 375, 528, 798
HpySE526I ACGT 2 cut(s) 241, 1075
Hsp92II CATG 5 cut(s) 97, 141, 855, 1122, 1229
HspAI GCGC 2 cut(s) 127, 546
Kzo9I GATC 5 cut(s) 620, 640, 1006, 1108, 1290
LmnI GCTCC 2 cut(s) 176, 368
LweI GCATC 4 cut(s) 655, 727, 951, 1171
MaeI CTAG 1 cut(s) 836
MaeII ACGT 2 cut(s) 241, 1075
MaeIII GTNAC 5 cut(s) 6, 233, 494, 776, 980
MalI GATC 5 cut(s) 622, 642, 1008, 1110, 1292
MboI GATC 5 cut(s) 620, 640, 1006, 1108, 1290
MboII GAAGA 5 cut(s) 625, 833, 915, 1285, 1300
MfeI CAATTG 2 cut(s) 453, 1210
MflI RGATCY 4 cut(s) 620, 640, 1006, 1290
MhlI GDGCHC 1 cut(s) 514
MlsI TGGCCA 1 cut(s) 573
MluCI AATT 4 cut(s) 444, 453, 708, 1210
MluI ACGCGT 1 cut(s) 311
MluNI TGGCCA 1 cut(s) 573
MlyI GAGTC 1 cut(s) 586
MmeI TCCRAC 1 cut(s) 1195
Mox20I TGGCCA 1 cut(s) 573
MroXI GAANNNNTTC 2 cut(s) 1045, 1292
MscI TGGCCA 1 cut(s) 573
MseI TTAA 6 cut(s) 677, 900, 935, 971, 1017, 1059
MslI CAYNNNNRTG 1 cut(s) 760
Msp20I TGGCCA 1 cut(s) 573
MspI CCGG 2 cut(s) 323, 730
MspR9I CCNGG 1 cut(s) 638
MunI CAATTG 2 cut(s) 453, 1210
MvaI CCWGG 1 cut(s) 638
MvnI CGCG 1 cut(s) 313
MwoI GCNNNNNNNGC 6 cut(s) 107, 138, 192, 195, 722, 1124
NcoI CCATGG 1 cut(s) 93
NdeI CATATG 1 cut(s) 718
NdeII GATC 5 cut(s) 620, 640, 1006, 1108, 1290
NlaIII CATG 5 cut(s) 97, 141, 855, 1122, 1229
NlaIV GGNNCC 2 cut(s) 795, 1260
NmuCI GTSAC 3 cut(s) 6, 233, 980
NspI RCATGY 1 cut(s) 141
PaeI GCATGC 1 cut(s) 141
PaqCI CACCTGC 1 cut(s) 403
PceI AGGCCT 1 cut(s) 770
PdmI GAANNNNTTC 2 cut(s) 1045, 1292
PfeI GAWTC 3 cut(s) 155, 684, 1046
PfoI TCCNGGA 1 cut(s) 636
PleI GAGTC 1 cut(s) 585
PpsI GAGTC 1 cut(s) 585
Ppu21I YACGTR 1 cut(s) 1076
PsiI TTATAA 1 cut(s) 33
Psp124BI GAGCTC 1 cut(s) 514
Psp1406I AACGTT 1 cut(s) 241
Psp6I CCWGG 1 cut(s) 636
PspGI CCWGG 1 cut(s) 636
PspN4I GGNNCC 2 cut(s) 795, 1260
PspPI GGNCC 1 cut(s) 793
PstI CTGCAG 1 cut(s) 628
PstNI CAGNNNCTG 2 cut(s) 643, 1100
PsuI RGATCY 4 cut(s) 620, 640, 1006, 1290
RsaI GTAC 3 cut(s) 301, 429, 1068
RsaNI GTAC 3 cut(s) 300, 428, 1067
RseI CAYNNNNRTG 1 cut(s) 760
SacI GAGCTC 1 cut(s) 514
SaqAI TTAA 6 cut(s) 677, 900, 935, 971, 1017, 1059
Sau3AI GATC 5 cut(s) 620, 640, 1006, 1108, 1290
Sau96I GGNCC 1 cut(s) 793
SchI GAGTC 1 cut(s) 586
ScrFI CCNGG 1 cut(s) 638
SduI GDGCHC 1 cut(s) 514
SfaNI GCATC 4 cut(s) 655, 727, 951, 1171
SfcI CTRYAG 3 cut(s) 624, 658, 693
SinI GGWCC 1 cut(s) 793
SmiMI CAYNNNNRTG 1 cut(s) 760
SphI GCATGC 1 cut(s) 141
Sse9I AATT 4 cut(s) 444, 453, 708, 1210
SseBI AGGCCT 1 cut(s) 770
SsiI CCGC 2 cut(s) 124, 366
SspI AATATT 1 cut(s) 1001
SspMI CTAG 1 cut(s) 836
SstI GAGCTC 1 cut(s) 514
StuI AGGCCT 1 cut(s) 770
StyD4I CCNGG 1 cut(s) 636
StyI CCWWGG 2 cut(s) 93, 271
TaaI ACNGT 4 cut(s) 233, 304, 339, 701
TaiI ACGT 2 cut(s) 244, 1078
TaqI TCGA 4 cut(s) 147, 257, 306, 959
TasI AATT 4 cut(s) 444, 453, 708, 1210
TatI WGTACW 1 cut(s) 1066
TauI GCSGC 1 cut(s) 127
TfiI GAWTC 3 cut(s) 155, 684, 1046
Tru1I TTAA 6 cut(s) 677, 900, 935, 971, 1017, 1059
Tru9I TTAA 6 cut(s) 677, 900, 935, 971, 1017, 1059
TscAI CASTG 1 cut(s) 610
TseFI GTSAC 3 cut(s) 6, 233, 980
Tsp45I GTSAC 3 cut(s) 6, 233, 980
TspDTI ATGAA 5 cut(s) 133, 200, 275, 306, 909
TspRI CASTG 1 cut(s) 610
VpaK11BI GGWCC 1 cut(s) 793
XceI RCATGY 1 cut(s) 141
XmiI GTMKAC 1 cut(s) 692
XmnI GAANNNNTTC 2 cut(s) 1045, 1292
XspI CTAG 1 cut(s) 836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.