MD06G1190000.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0017305
Physical Location & Seq
Reverse (-)
32642239 .. 32642829
591 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1190000.v1.1.491

Sequence Viewer

Length: 591 bp
ATGTCAAGCAACCGAGCCTTTGCACGCTGTGAAGTATGGCAGGAAGGCAGCGAACTCGCCGACGACAGTTCCTCGACGAAACCCGAGTTCTTGATTGATATGTATGCTCAATTTTCAAGGTACCCTCTAAGCTTCAAGGAGCAATGCCACGTTCTAACCGAGAGCAAAACATCCTGGTCTGTAATATCCAACATGCTTTCGCAGGCGTCAGTTCCCTACCAAGTTCAGCCGTCCATGATCCTGGCGATTTCCACCCGCGCTCGTGAAATCGCCCGTGATCCTGAGAACAAGAACCGGAAGACTATTCGGATGGTAGTGCAACTAACAGTTGAACAAGAACAGTTGTCGGAGGACCACGACCAAACGTTTGTGCCGCGGACAAAAGGTACACCAATTGAAGAGCTAGAGAGAGTCAAAATCGATGTCAGCGGGAAGCAATGTGTTATATGTTTGGAAGAGATGCAGCGTGGTTGTGAATCGATTCGCATGCCTTGCTCGCATGACTACCACGAGAGTTGCATAACGAATTGGCTGGAGACAAGTCACCTGTGCCCGCTGTGCCGGTTTGAGATGCCCGCTGCATCCTTGTGA

Protein Analysis

197

Amino Acids

22.49

Weight (kDa)

5.3

Isoelectric Point (pI)

52.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 145 - 188 6.2e-10 RING-H2 zinc finger domain
zf-RING_2 PF13639 146 - 188 4.5e-13 Ring finger domain
zf-RING_11 PF17123 146 - 174 1.9e-07 RING-like zinc finger
zf-C3HC4_2 PF13923 146 - 187 4.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 147 - 187 1.6e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017305)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g15260
malus_domestica MD06G1190000.v1.1 MD14G1196800.v1.1
prunus_persica Prupe.5G189000_v2.0.a1
pyrus_communis pycom06g16870
rosa_chinensis RchiOBHm_Chr7g0180831
rosa_multiflora Rmu_sc0007457.1_g000005
rosa_roxburghii Rroxscaffold_3G00272490
rosa_rugosa Rorug06G0436700
rosa_samantha Rh7AG038300 Rh7CG039500 Rh7DG038000
rosa_wichuraiana Rw7G003150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 120
AccB1I GGYRCC 1 cut(s) 120
AccII CGCG 2 cut(s) 258, 376
AciI CCGC 6 cut(s) 256, 374, 376, 429, 554, 576
AclI AACGTT 1 cut(s) 365
AclWI GGATC 2 cut(s) 232, 272
AcyI GRCGYC 1 cut(s) 206
AdeI CACNNNGTG 1 cut(s) 29
AfaI GTAC 2 cut(s) 122, 388
AgsI TTSAA 4 cut(s) 117, 136, 332, 398
AjnI CCWGG 2 cut(s) 173, 240
AluBI AGCT 2 cut(s) 132, 403
AluI AGCT 2 cut(s) 132, 403
Alw26I GTCTC 1 cut(s) 530
AlwI GGATC 2 cut(s) 232, 272
Ama87I CYCGRG 1 cut(s) 83
ApeKI GCWGC 3 cut(s) 48, 463, 578
ArsI GACNNNNNNTTYG 4 cut(s) 350, 382, 408, 440
Asp700I GAANNNNTTC 1 cut(s) 480
Asp718I GGTACC 1 cut(s) 120
AspLEI GCGC 1 cut(s) 260
AspS9I GGNCC 1 cut(s) 352
AsuHPI GGTGA 1 cut(s) 536
AvaI CYCGRG 1 cut(s) 83
AvaII GGWCC 1 cut(s) 352
BaeGI GKGCMC 1 cut(s) 554
BanI GGYRCC 1 cut(s) 120
BauI CACGAG 2 cut(s) 261, 509
BbsI GAAGAC 1 cut(s) 305
BbvI GCAGC 3 cut(s) 60, 475, 565
BccI CCATC 1 cut(s) 304
BceAI ACGGC 1 cut(s) 214
BcgI CGANNNNNNTGC 4 cut(s) 37, 71, 469, 503
BciT130I CCWGG 2 cut(s) 175, 242
BcoDI GTCTC 1 cut(s) 530
BfaI CTAG 1 cut(s) 404
BisI GCNGC 4 cut(s) 49, 374, 464, 579
BlsI GCNGC 4 cut(s) 50, 375, 465, 580
Bme1390I CCNGG 2 cut(s) 175, 242
Bme18I GGWCC 1 cut(s) 352
BmeT110I CYCGRG 1 cut(s) 83
BmgT120I GGNCC 1 cut(s) 352
BmiI GGNNCC 1 cut(s) 122
BmrFI CCNGG 2 cut(s) 175, 242
BmsI GCATC 2 cut(s) 450, 561
BpiI GAAGAC 1 cut(s) 305
BpmI CTGGAG 1 cut(s) 554
Bsa29I ATCGAT 2 cut(s) 420, 479
BsaHI GRCGYC 1 cut(s) 206
BsaJI CCNNGG 1 cut(s) 374
BsaWI WCCGGW 1 cut(s) 294
Bse118I RCCGGY 1 cut(s) 561
Bse3DI GCAATG 2 cut(s) 149, 443
BseBI CCWGG 2 cut(s) 175, 242
BseCI ATCGAT 2 cut(s) 420, 479
BseDI CCNNGG 1 cut(s) 374
BseGI GGATG 3 cut(s) 170, 315, 581
BseMI GCAATG 2 cut(s) 149, 443
BseMII CTCAG 1 cut(s) 273
BseSI GKGCMC 1 cut(s) 554
BseXI GCAGC 3 cut(s) 60, 475, 565
Bsh1236I CGCG 2 cut(s) 258, 376
BshNI GGYRCC 1 cut(s) 120
BshVI ATCGAT 2 cut(s) 420, 479
BsiHKCI CYCGRG 1 cut(s) 83
BsiSI CCGG 2 cut(s) 295, 562
BsmAI GTCTC 1 cut(s) 530
BsoBI CYCGRG 1 cut(s) 83
Bsp1286I GDGCHC 1 cut(s) 554
Bsp143I GATC 2 cut(s) 237, 277
BspACI CCGC 6 cut(s) 256, 374, 376, 429, 554, 576
BspCNI CTCAG 1 cut(s) 274
BspDI ATCGAT 2 cut(s) 420, 479
BspFNI CGCG 2 cut(s) 258, 376
BspLI GGNNCC 1 cut(s) 122
BspPI GGATC 2 cut(s) 232, 272
BspQI GCTCTTC 1 cut(s) 393
BspT107I GGYRCC 1 cut(s) 120
BsrDI GCAATG 2 cut(s) 149, 443
BsrFI RCCGGY 1 cut(s) 561
BssAI RCCGGY 1 cut(s) 561
BssECI CCNNGG 1 cut(s) 374
BssMI GATC 2 cut(s) 237, 277
BssNI GRCGYC 1 cut(s) 206
BssSI CACGAG 2 cut(s) 261, 509
Bst2BI CACGAG 2 cut(s) 261, 509
Bst2UI CCWGG 2 cut(s) 175, 242
Bst4CI ACNGT 3 cut(s) 68, 328, 342
Bst6I CTCTTC 2 cut(s) 393, 450
BstACI GRCGYC 1 cut(s) 206
BstAPI GCANNNNNTGC 1 cut(s) 492
BstC8I GCNNGC 6 cut(s) 25, 204, 488, 497, 554, 576
BstDEI CTNAG 2 cut(s) 128, 282
BstDSI CCRYGG 1 cut(s) 374
BstF5I GGATG 3 cut(s) 170, 315, 581
BstFNI CGCG 2 cut(s) 258, 376
BstHHI GCGC 1 cut(s) 260
BstKTI GATC 2 cut(s) 240, 280
BstMAI GTCTC 1 cut(s) 530
BstMBI GATC 2 cut(s) 237, 277
BstMWI GCNNNNNNNGC 3 cut(s) 492, 496, 558
BstNI CCWGG 2 cut(s) 175, 242
BstNSI RCATGY 2 cut(s) 196, 490
BstSCI CCNGG 2 cut(s) 173, 240
BstSLI GKGCMC 1 cut(s) 554
BstUI CGCG 2 cut(s) 258, 376
BstV1I GCAGC 3 cut(s) 60, 475, 565
BstV2I GAAGAC 1 cut(s) 305
BstXI CCANNNNNNTGG 1 cut(s) 241
Bsu15I ATCGAT 2 cut(s) 420, 479
BsuTUI ATCGAT 2 cut(s) 420, 479
BtgI CCRYGG 1 cut(s) 374
BtsCI GGATG 3 cut(s) 170, 315, 581
Cac8I GCNNGC 6 cut(s) 25, 204, 488, 497, 554, 576
CfoI GCGC 1 cut(s) 260
Cfr10I RCCGGY 1 cut(s) 561
Cfr13I GGNCC 1 cut(s) 352
Cfr42I CCGCGG 1 cut(s) 377
ClaI ATCGAT 2 cut(s) 420, 479
CseI GACGC 1 cut(s) 195
Csp6I GTAC 2 cut(s) 121, 387
CspCI CAANNNNNGTGG 2 cut(s) 497, 532
CviAII CATG 4 cut(s) 193, 235, 487, 500
CviJI RGCY 5 cut(s) 17, 132, 229, 403, 532
CviKI_1 RGCY 5 cut(s) 17, 132, 229, 403, 532
CviQI GTAC 2 cut(s) 121, 387
DdeI CTNAG 2 cut(s) 128, 282
DpnI GATC 2 cut(s) 239, 279
DpnII GATC 2 cut(s) 237, 277
DraIII CACNNNGTG 1 cut(s) 29
Eam1104I CTCTTC 2 cut(s) 393, 450
EarI CTCTTC 2 cut(s) 393, 450
Eco47I GGWCC 1 cut(s) 352
Eco88I CYCGRG 1 cut(s) 83
EcoRII CCWGG 2 cut(s) 173, 240
FaeI CATG 4 cut(s) 196, 238, 490, 503
FatI CATG 4 cut(s) 192, 234, 486, 499
FauI CCCGC 4 cut(s) 263, 422, 561, 583
Fnu4HI GCNGC 4 cut(s) 49, 374, 464, 579
FokI GGATG 3 cut(s) 157, 322, 568
Fsp4HI GCNGC 4 cut(s) 49, 374, 464, 579
FspBI CTAG 1 cut(s) 404
GlaI GCGC 1 cut(s) 259
GluI GCNGC 4 cut(s) 49, 374, 464, 579
GsuI CTGGAG 1 cut(s) 554
HapII CCGG 2 cut(s) 295, 562
HgaI GACGC 1 cut(s) 195
HhaI GCGC 1 cut(s) 260
Hin1I GRCGYC 1 cut(s) 206
Hin1II CATG 4 cut(s) 196, 238, 490, 503
Hin6I GCGC 1 cut(s) 258
HinP1I GCGC 1 cut(s) 258
HindIII AAGCTT 1 cut(s) 130
HinfI GANTC 3 cut(s) 411, 476, 481
HpaII CCGG 2 cut(s) 295, 562
HphI GGTGA 1 cut(s) 536
Hpy166II GTNNAC 1 cut(s) 389
Hpy188I TCNGA 2 cut(s) 309, 349
Hpy188III TCNNGA 3 cut(s) 91, 263, 281
Hpy8I GTNNAC 1 cut(s) 389
Hpy99I CGWCG 2 cut(s) 65, 79
HpyAV CCTTC 1 cut(s) 38
HpyCH4III ACNGT 3 cut(s) 68, 328, 342
HpyCH4IV ACGT 2 cut(s) 150, 365
HpyCH4V TGCA 5 cut(s) 23, 319, 463, 519, 581
HpyF10VI GCNNNNNNNGC 3 cut(s) 492, 496, 558
HpyF3I CTNAG 2 cut(s) 128, 282
HpySE526I ACGT 2 cut(s) 150, 365
Hsp92I GRCGYC 1 cut(s) 206
Hsp92II CATG 4 cut(s) 196, 238, 490, 503
HspAI GCGC 1 cut(s) 258
KpnI GGTACC 1 cut(s) 124
KspI CCGCGG 1 cut(s) 377
Kzo9I GATC 2 cut(s) 237, 277
LguI GCTCTTC 1 cut(s) 393
LmnI GCTCC 1 cut(s) 139
Lsp1109I GCAGC 3 cut(s) 60, 475, 565
LweI GCATC 2 cut(s) 450, 561
MaeI CTAG 1 cut(s) 404
MaeII ACGT 2 cut(s) 150, 365
MaeIII GTNAC 1 cut(s) 542
MalI GATC 2 cut(s) 239, 279
MboI GATC 2 cut(s) 237, 277
MboII GAAGA 3 cut(s) 310, 410, 467
MfeI CAATTG 1 cut(s) 393
MhlI GDGCHC 1 cut(s) 554
MluCI AATT 3 cut(s) 110, 393, 526
MlyI GAGTC 1 cut(s) 420
MmeI TCCRAC 2 cut(s) 213, 327
MnlI CCTC 3 cut(s) 82, 135, 343
MroXI GAANNNNTTC 1 cut(s) 480
MslI CAYNNNNRTG 1 cut(s) 586
MspA1I CMGCKG 4 cut(s) 376, 429, 556, 578
MspI CCGG 2 cut(s) 295, 562
MspR9I CCNGG 2 cut(s) 175, 242
MunI CAATTG 1 cut(s) 393
MvaI CCWGG 2 cut(s) 175, 242
MvnI CGCG 2 cut(s) 258, 376
MwoI GCNNNNNNNGC 3 cut(s) 492, 496, 558
NdeII GATC 2 cut(s) 237, 277
NlaIII CATG 4 cut(s) 196, 238, 490, 503
NlaIV GGNNCC 1 cut(s) 122
NmuCI GTSAC 1 cut(s) 542
NspI RCATGY 2 cut(s) 196, 490
PaeI GCATGC 1 cut(s) 490
PciSI GCTCTTC 1 cut(s) 393
PcsI WCGNNNNNNNCGW 1 cut(s) 156
PdmI GAANNNNTTC 1 cut(s) 480
PfeI GAWTC 2 cut(s) 476, 481
PkrI GCNGC 4 cut(s) 50, 375, 465, 580
PleI GAGTC 1 cut(s) 419
PpsI GAGTC 1 cut(s) 419
Psp1406I AACGTT 1 cut(s) 365
Psp6I CCWGG 2 cut(s) 173, 240
PspGI CCWGG 2 cut(s) 173, 240
PspN4I GGNNCC 1 cut(s) 122
PspPI GGNCC 1 cut(s) 352
RsaI GTAC 2 cut(s) 122, 388
RsaNI GTAC 2 cut(s) 121, 387
RseI CAYNNNNRTG 1 cut(s) 586
SacII CCGCGG 1 cut(s) 377
SapI GCTCTTC 1 cut(s) 393
SatI GCNGC 4 cut(s) 49, 374, 464, 579
Sau3AI GATC 2 cut(s) 237, 277
Sau96I GGNCC 1 cut(s) 352
SchI GAGTC 1 cut(s) 420
ScrFI CCNGG 2 cut(s) 175, 242
SduI GDGCHC 1 cut(s) 554
SetI ASST 7 cut(s) 122, 134, 153, 368, 388, 405, 549
SfaNI GCATC 2 cut(s) 450, 561
Sfr303I CCGCGG 1 cut(s) 377
SgrBI CCGCGG 1 cut(s) 377
SinI GGWCC 1 cut(s) 352
SmiMI CAYNNNNRTG 1 cut(s) 586
SphI GCATGC 1 cut(s) 490
Sse9I AATT 3 cut(s) 110, 393, 526
SsiI CCGC 6 cut(s) 256, 374, 376, 429, 554, 576
SspMI CTAG 1 cut(s) 404
StyD4I CCNGG 2 cut(s) 173, 240
TaaI ACNGT 3 cut(s) 68, 328, 342
TaiI ACGT 2 cut(s) 153, 368
TaqI TCGA 3 cut(s) 74, 420, 479
TasI AATT 3 cut(s) 110, 393, 526
TauI GCSGC 1 cut(s) 376
TfiI GAWTC 2 cut(s) 476, 481
TseFI GTSAC 1 cut(s) 542
TseI GCWGC 3 cut(s) 48, 463, 578
Tsp45I GTSAC 1 cut(s) 542
VpaK11BI GGWCC 1 cut(s) 352
XceI RCATGY 2 cut(s) 196, 490
XmnI GAANNNNTTC 1 cut(s) 480
XspI CTAG 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.