Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0009313

Physical Location & Seq

Genomic Coordinates Reverse (-)

34735129 .. 34738991

Gene Structure 3863 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1214800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1809 bp

ATGGCAGCAAAATTGGGTTCCTTCAAGTACACTTTCCAGGAGAAGAGAGAGCGGTTGCTCTCCGTCCAGAAGGGCTACTCCTACTCCGAGCTGGGTGGCTTCCCCAACATCGAGGAGCAAGACCCGTCGTCGGGGTCTACCCGCTGCTGCACTTTCCGATCAGTCTCTGACCGAATTGTCGGCTGGTGCCGAACCGTGCAACATGTCTCGAAACGGGCCGTTAAGATGGGTCAGTCCGATCCGAGAAAGATCATCTTCGCCGCGAAGATGGGTCTGGCGCTGATGATCATCTCGCTGCTCATCTTCCTCAAAGAGCCGTTCAAGCAGCTCAGCCGCTACTCCGTCTGGGCAATTCTTACCGTTGTGGTGGTGTTCGAGTTCAGCATAGGAGCGACTCTGAGCAAAGGATTTAACCGCGGTTTGGGGACCCTTTCGGCTGGAGGCCTGGCTTTGGTTGTGGCAAACTTATGTGAGCTAGCTGGAGAGTGGGAAGAAATCGTTATTTTCGCTAGCATCTTTATCGTAGGATTTCTTGCAACTTATGCGAAGCTATACCCGACAATGAAGCCTTATGAATATGGGTTTCGGGTGTTCTTGTTGACATTTTGTTTCATCATGGTATCTGGGTATCGAACGAGGGAATTTCTTCATACAGCTGTGTCTCGATTCTTTCTCATTGCACTGGGTGCTGGTGTTGGTTTGGGAGTAAATATATGCATTTTTCCCATCTGGGCTGGCGAGGATCTGCACAACTTGGTGGTAAAAAATTTCCTGGGTGTTGCAAAATCACTCGAAGGTGTTGTTAACAGTTACCTTAATTGCGTTGAATATGAGAGGGTCCCTTCCAAGATTCTTACCTATCAAGCTTCAGATGATCCACTCTATAGTGGCTACAGATCTGCTGTGGAATCTAGAAGCCAAGAGGACACTCTGATGGGATTTGCTATCTGGGAGCCACCACATGGTCGATACAGAATGCTTAGATATCCATGGAAGAACTATGCAAAAGTGGGTGGTGCACTGAGGCATTGTGCATTTATGGTCATGGCATTGCACGGATGTATACTTTCTGAAATACAGGCTCCTGCTGAGCGAAGACAAGTATTTCGTAAGGAACTTCAAAGGGTAGGTTATGAAGGTGCCAAAGTTTTACGTGAACTTGGAAACAAACTGAAAACAATGGAGAAAATAGGTCCCATAGATATACTCAATGAAGTGCACGAGGCTGCAGAAGACTTACAAAAGAAAGTTGACCAGAAGTCGTACCTGCTTGTTAATTCAGAAGGCTGGGAGATTGGAAGTCGACCAAAGGAGCTGGGAGAACCTCAGGACTTACTGAACGTGGATGATGAAGAAAATTATTTCCGGGAATACAAATCCCTTAGTGAAGCTGTACTTGATCTCAGTTCTTTTCCTGCGCCTCAAAGTTGGGATAGCCAGATGCCCGCAAAGCCAAATGGCCCAGGCTCCATACCAACTGAAGTCAACCACTCGAACTTGCCCACAGGTGTTTCGTCAGGAAGCATGTTCATGAAACAGATATCGTGGCCTGCAGGACTTAAATTTAAGGCTGAGGAACCGCCAGTGGCGGAGGAATCCAAAACCTTTGAAAATGCTAGTCAGTTGTCGTTAGCCACATTTACATCCCTGTTGATAGAGTTTGTCGCAAGGCTTCAAAATCTCGTAGACTCATTTGAAGAACTAAGTGAGAAAGCACATTTCAAGGAACCTGCCGAACCACCAGAAATATTACAGCCACCTCGCAGTCACAGGTTTTGGACCCGGTTGCTCAACTGTCTGAAATCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0003674 GO:0005215 GO:0005216 GO:0005253 GO:0005310 GO:0005342 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0006811 GO:0006820 GO:0006835 GO:0008150 GO:0008509 GO:0008514 GO:0009705 GO:0015075 GO:0015103 GO:0015140 GO:0015267 GO:0015318 GO:0015556 GO:0015698 GO:0015711 GO:0015740 GO:0015743 GO:0015849 GO:0016020 GO:0022803 GO:0022838 GO:0022857 GO:0031090 GO:0034220 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046942 GO:0046943 GO:0051179 GO:0051234 GO:0055085 GO:0071423 GO:0071702 GO:0098588 GO:0098656 GO:0098805 GO:1903825 GO:1905039

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ALMT

Protein Analysis

603

Amino Acids

67.64

Weight (kDa)

7.92

Isoelectric Point (pI)

43.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ALMT	PF11744	75 - 569	1.9e-190	Aluminium activated malate transporter
FUSC	PF04632	97 - 268	3.1e-06	Fusaric acid resistance protein family
FUSC_2	PF13515	113 - 234	4.5e-11	Fusaric acid resistance protein-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009313)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G18440
fragaria_vesca	FvH4_5g12330 FvH4_5g12330 FvH4_5g12330 FvH4_5g12330 FvH4_5g12330
malus_domestica	MD06G1214800.v1.1 MD14G1225700.v1.1
prunus_persica	Prupe.5G220200_v2.0.a1
pyrus_communis	pycom06g19140 pycom14g18690
rosa_chinensis	RchiOBHm_Chr7g0185231
rosa_laevigata	RLG00000004929
rosa_multiflora	Rmu_sc0000213.1_g000005 Rmu_sc0008496.1_g000019
rosa_roxburghii	Rroxscaffold_3G00269120
rosa_rugosa	Rorug06G0467100
rosa_samantha	Rh7AG075800 Rh7BG071600 Rh7CG072800 Rh7DG072200
rosa_wichuraiana	Rw7G006000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	176
Acc36I	ACCTGC	2 cut(s)	1275, 1738
AccB1I	GGYRCC	2 cut(s)	186, 1139
AccB7I	CCANNNNNTGG	1 cut(s)	962
AccBSI	CCGCTC	1 cut(s)	52
AccI	GTMKAC	4 cut(s)	137, 1063, 1303, 1686
AccII	CGCG	2 cut(s)	263, 417
AciI	CCGC	9 cut(s)	52, 142, 261, 334, 415, 417, 1446, 1580, 1589
AclWI	GGATC	3 cut(s)	233, 750, 869
AcsI	RAATTY	3 cut(s)	641, 766, 1562
AcuI	CTGAAG	2 cut(s)	852, 1500
AdeI	CACNNNGTG	1 cut(s)	686
AfaI	GTAC	3 cut(s)	29, 1265, 1395
AfiI	CCNNNNNNNGG	5 cut(s)	130, 131, 421, 451, 962
AflIII	ACRYGT	1 cut(s)	202
AgsI	TTSAA	8 cut(s)	25, 322, 827, 1121, 1610, 1676, 1697, 1723
AhdI	GACNNNNNGTC	2 cut(s)	127, 1258
AjnI	CCWGG	4 cut(s)	36, 444, 771, 1462
AluBI	AGCT	9 cut(s)	91, 328, 475, 479, 550, 656, 866, 1315, 1391
AluI	AGCT	9 cut(s)	91, 328, 475, 479, 550, 656, 866, 1315, 1391
Alw21I	GWGCWC	2 cut(s)	1021, 1221
Alw26I	GTCTC	3 cut(s)	169, 211, 666
Alw44I	GTGCAC	2 cut(s)	1017, 1217
AlwI	GGATC	3 cut(s)	233, 750, 869
AlwNI	CAGNNNCTG	1 cut(s)	167
AoxI	GGCC	4 cut(s)	216, 442, 1459, 1547
ApaLI	GTGCAC	2 cut(s)	1017, 1217
ApeKI	GCWGC	6 cut(s)	5, 144, 147, 295, 325, 1226
ApoI	RAATTY	3 cut(s)	641, 766, 1562
ArsI	GACNNNNNNTTYG	2 cut(s)	1089, 1121
Asp700I	GAANNNNTTC	1 cut(s)	645
AspLEI	GCGC	2 cut(s)	280, 1420
AspS9I	GGNCC	6 cut(s)	216, 426, 838, 1193, 1460, 1779
AsuC2I	CCSGG	2 cut(s)	1367, 1783
AsuNHI	GCTAGC	2 cut(s)	475, 509
AvaII	GGWCC	4 cut(s)	426, 838, 1193, 1779
AxyI	CCTNAGG	1 cut(s)	1326
BaeGI	GKGCMC	2 cut(s)	1021, 1221
BanI	GGYRCC	2 cut(s)	186, 1139
BauI	CACGAG	1 cut(s)	1220
BbsI	GAAGAC	2 cut(s)	1102, 1239
Bbv12I	GWGCWC	2 cut(s)	1021, 1221
BbvCI	CCTCAGC	1 cut(s)	1572
BbvI	GCAGC	6 cut(s)	17, 131, 134, 282, 337, 1213
BccI	CCATC	4 cut(s)	220, 262, 734, 928
BceAI	ACGGC	2 cut(s)	203, 301
BcgI	CGANNNNNNTGC	2 cut(s)	502, 536
BciT130I	CCWGG	4 cut(s)	38, 446, 773, 1464
BclI	TGATCA	1 cut(s)	285
BcnI	CCSGG	2 cut(s)	1367, 1783
BcoDI	GTCTC	3 cut(s)	169, 211, 666
BfaI	CTAG	4 cut(s)	476, 510, 912, 1617
BfmI	CTRYAG	4 cut(s)	883, 892, 1227, 1551
BfoI	RGCGCY	1 cut(s)	281
BfuAI	ACCTGC	2 cut(s)	1275, 1738
BglII	AGATCT	1 cut(s)	896
BisI	GCNGC	8 cut(s)	6, 145, 148, 261, 296, 326, 334, 1227
BlpI	GCTNAGC	2 cut(s)	329, 1089
BlsI	GCNGC	8 cut(s)	7, 146, 149, 262, 297, 327, 335, 1228
Bme1390I	CCNGG	6 cut(s)	38, 446, 773, 1367, 1464, 1783
Bme18I	GGWCC	4 cut(s)	426, 838, 1193, 1779
BmeRI	GACNNNNNGTC	2 cut(s)	127, 1258
BmgT120I	GGNCC	6 cut(s)	216, 426, 838, 1193, 1460, 1779
BmrFI	CCNGG	6 cut(s)	38, 446, 773, 1367, 1464, 1783
BmrI	ACTGGG	1 cut(s)	692
BmsI	GCATC	2 cut(s)	522, 1431
BmtI	GCTAGC	2 cut(s)	479, 513
BmuI	ACTGGG	1 cut(s)	692
BpiI	GAAGAC	2 cut(s)	1102, 1239
BpmI	CTGGAG	2 cut(s)	459, 501
Bpu10I	CCTNAGC	1 cut(s)	1572
Bpu1102I	GCTNAGC	2 cut(s)	329, 1089
BpuMI	CCSGG	2 cut(s)	1367, 1783
BsaAI	YACGTR	1 cut(s)	1154
BsaBI	GATNNNNATC	1 cut(s)	287
BsaJI	CCNNGG	4 cut(s)	415, 772, 989, 1462
BsaXI	ACNNNNNCTCC	2 cut(s)	68, 98
Bsc4I	CCNNNNNNNGG	5 cut(s)	130, 131, 421, 451, 962
Bse1I	ACTGG	2 cut(s)	687, 1583
Bse21I	CCTNAGG	1 cut(s)	1326
Bse3DI	GCAATG	2 cut(s)	675, 1049
Bse8I	GATNNNNATC	1 cut(s)	287
BseBI	CCWGG	4 cut(s)	38, 446, 773, 1464
BseDI	CCNNGG	4 cut(s)	415, 772, 989, 1462
BseGI	GGATG	3 cut(s)	1064, 1351, 1643
BseJI	GATNNNNATC	1 cut(s)	287
BseLI	CCNNNNNNNGG	5 cut(s)	130, 131, 421, 451, 962
BseMI	GCAATG	2 cut(s)	675, 1049
BseMII	CTCAG	7 cut(s)	343, 389, 1013, 1080, 1340, 1417, 1563
BseNI	ACTGG	2 cut(s)	687, 1583
BseRI	GAGGAG	1 cut(s)	128
BseSI	GKGCMC	2 cut(s)	1021, 1221
BseXI	GCAGC	6 cut(s)	17, 131, 134, 282, 337, 1213
BseYI	CCCAGC	3 cut(s)	91, 1287, 1315
BsgI	GTGCAG	2 cut(s)	133, 731
Bsh1236I	CGCG	2 cut(s)	263, 417
BshFI	GGCC	4 cut(s)	218, 444, 1461, 1549
BshNI	GGYRCC	2 cut(s)	186, 1139
BsiHKAI	GWGCWC	2 cut(s)	1021, 1221
BsiSI	CCGG	2 cut(s)	1366, 1783
BslFI	GGGAC	3 cut(s)	439, 824, 1179
BslI	CCNNNNNNNGG	5 cut(s)	130, 131, 421, 451, 962
BsmAI	GTCTC	3 cut(s)	169, 211, 666
BsmFI	GGGAC	3 cut(s)	439, 824, 1179
BsmI	GAATGC	1 cut(s)	981
BsnI	GGCC	4 cut(s)	218, 444, 1461, 1549
Bsp1286I	GDGCHC	2 cut(s)	1021, 1221
Bsp143I	GATC	8 cut(s)	158, 238, 249, 285, 742, 874, 896, 1399
Bsp1720I	GCTNAGC	2 cut(s)	329, 1089
Bsp19I	CCATGG	1 cut(s)	989
BspACI	CCGC	9 cut(s)	52, 142, 261, 334, 415, 417, 1446, 1580, 1589
BspANI	GGCC	4 cut(s)	218, 444, 1461, 1549
BspCNI	CTCAG	7 cut(s)	342, 390, 1014, 1081, 1339, 1416, 1564
BspFNI	CGCG	2 cut(s)	263, 417
BspHI	TCATGA	1 cut(s)	1530
BspMAI	CTGCAG	2 cut(s)	1231, 1555
BspMI	ACCTGC	2 cut(s)	1275, 1738
BspOI	GCTAGC	2 cut(s)	479, 513
BspPI	GGATC	3 cut(s)	233, 750, 869
BspT107I	GGYRCC	2 cut(s)	186, 1139
BsrBI	CCGCTC	1 cut(s)	52
BsrDI	GCAATG	2 cut(s)	675, 1049
BsrI	ACTGG	2 cut(s)	687, 1583
BssECI	CCNNGG	4 cut(s)	415, 772, 989, 1462
BssMI	GATC	8 cut(s)	158, 238, 249, 285, 742, 874, 896, 1399
BssNAI	GTATAC	1 cut(s)	1064
BssSI	CACGAG	1 cut(s)	1220
BssT1I	CCWWGG	1 cut(s)	989
Bst1107I	GTATAC	1 cut(s)	1064
Bst2BI	CACGAG	1 cut(s)	1220
Bst2UI	CCWGG	4 cut(s)	38, 446, 773, 1464
Bst4CI	ACNGT	4 cut(s)	196, 361, 809, 1796
Bst6I	CTCTTC	1 cut(s)	38
BstAPI	GCANNNNNTGC	2 cut(s)	542, 686
BstBAI	YACGTR	1 cut(s)	1154
BstC8I	GCNNGC	5 cut(s)	477, 511, 736, 1446, 1551
BstDSI	CCRYGG	2 cut(s)	415, 989
BstF5I	GGATG	3 cut(s)	1064, 1351, 1643
BstFNI	CGCG	2 cut(s)	263, 417
BstH2I	RGCGCY	1 cut(s)	281
BstHHI	GCGC	2 cut(s)	280, 1420
BstKTI	GATC	8 cut(s)	161, 241, 252, 288, 745, 877, 899, 1402
BstMAI	GTCTC	3 cut(s)	169, 211, 666
BstMBI	GATC	8 cut(s)	158, 238, 249, 285, 742, 874, 896, 1399
BstMWI	GCNNNNNNNGC	4 cut(s)	322, 542, 686, 1450
BstNI	CCWGG	4 cut(s)	38, 446, 773, 1464
BstNSI	RCATGY	2 cut(s)	206, 1528
BstSCI	CCNGG	6 cut(s)	36, 444, 771, 1365, 1462, 1781
BstSFI	CTRYAG	4 cut(s)	883, 892, 1227, 1551
BstSLI	GKGCMC	2 cut(s)	1021, 1221
BstUI	CGCG	2 cut(s)	263, 417
BstV1I	GCAGC	6 cut(s)	17, 131, 134, 282, 337, 1213
BstV2I	GAAGAC	2 cut(s)	1102, 1239
BstX2I	RGATCY	2 cut(s)	742, 896
BstYI	RGATCY	2 cut(s)	742, 896
BstZ17I	GTATAC	1 cut(s)	1064
Bsu36I	CCTNAGG	1 cut(s)	1326
BsuRI	GGCC	4 cut(s)	218, 444, 1461, 1549
BtgI	CCRYGG	2 cut(s)	415, 989
BtsCI	GGATG	3 cut(s)	1064, 1351, 1643
BtsIMutI	CAGTG	3 cut(s)	680, 1019, 1590
BveI	ACCTGC	2 cut(s)	1275, 1738
Cac8I	GCNNGC	5 cut(s)	477, 511, 736, 1446, 1551
CaiI	CAGNNNCTG	1 cut(s)	167
CciI	TCATGA	1 cut(s)	1530
CfoI	GCGC	2 cut(s)	280, 1420
Cfr13I	GGNCC	6 cut(s)	216, 426, 838, 1193, 1460, 1779
Cfr42I	CCGCGG	1 cut(s)	418
Csp6I	GTAC	3 cut(s)	28, 1264, 1394
CviAII	CATG	7 cut(s)	203, 616, 962, 990, 1045, 1525, 1531
CviQI	GTAC	3 cut(s)	28, 1264, 1394
DpnI	GATC	8 cut(s)	160, 240, 251, 287, 744, 876, 898, 1401
DpnII	GATC	8 cut(s)	158, 238, 249, 285, 742, 874, 896, 1399
DraIII	CACNNNGTG	1 cut(s)	686
DrdI	GACNNNNNNGTC	1 cut(s)	176
DriI	GACNNNNNGTC	2 cut(s)	127, 1258
DseDI	GACNNNNNNGTC	1 cut(s)	176
Eam1104I	CTCTTC	1 cut(s)	38
Eam1105I	GACNNNNNGTC	2 cut(s)	127, 1258
EarI	CTCTTC	1 cut(s)	38
EciI	GGCGGA	1 cut(s)	1604
Eco130I	CCWWGG	1 cut(s)	989
Eco147I	AGGCCT	1 cut(s)	444
Eco32I	GATATC	2 cut(s)	986, 1542
Eco47I	GGWCC	4 cut(s)	426, 838, 1193, 1779
Eco57I	CTGAAG	2 cut(s)	852, 1500
Eco81I	CCTNAGG	1 cut(s)	1326
EcoO109I	RGGNCCY	3 cut(s)	426, 838, 1193
EcoRII	CCWGG	4 cut(s)	36, 444, 771, 1462
EcoRV	GATATC	2 cut(s)	986, 1542
EcoT14I	CCWWGG	1 cut(s)	989
EcoT22I	ATGCAT	1 cut(s)	719
ErhI	CCWWGG	1 cut(s)	989
FaeI	CATG	7 cut(s)	206, 619, 965, 993, 1048, 1528, 1534
FalI	AAGNNNNNCTT	4 cut(s)	239, 271, 1380, 1412
FaqI	GGGAC	3 cut(s)	439, 824, 1179
FatI	CATG	7 cut(s)	202, 615, 961, 989, 1044, 1524, 1530
FauI	CCCGC	2 cut(s)	149, 1453
FbaI	TGATCA	1 cut(s)	285
FblI	GTMKAC	4 cut(s)	137, 1063, 1303, 1686
Fnu4HI	GCNGC	8 cut(s)	6, 145, 148, 261, 296, 326, 334, 1227
FokI	GGATG	3 cut(s)	1071, 1358, 1630
Fsp4HI	GCNGC	8 cut(s)	6, 145, 148, 261, 296, 326, 334, 1227
FspBI	CTAG	4 cut(s)	476, 510, 912, 1617
GlaI	GCGC	2 cut(s)	279, 1419
GluI	GCNGC	8 cut(s)	6, 145, 148, 261, 296, 326, 334, 1227
GsaI	CCCAGC	3 cut(s)	95, 1291, 1319
GsuI	CTGGAG	2 cut(s)	459, 501
HaeII	RGCGCY	1 cut(s)	281
HaeIII	GGCC	4 cut(s)	218, 444, 1461, 1549
HapII	CCGG	2 cut(s)	1366, 1783
HhaI	GCGC	2 cut(s)	280, 1420
Hin1II	CATG	7 cut(s)	206, 619, 965, 993, 1048, 1528, 1534
Hin6I	GCGC	2 cut(s)	278, 1418
HinP1I	GCGC	2 cut(s)	278, 1418
HincII	GTYRAC	5 cut(s)	600, 805, 1252, 1304, 1486
HindII	GTYRAC	5 cut(s)	600, 805, 1252, 1304, 1486
HindIII	AAGCTT	1 cut(s)	864
HinfI	GANTC	6 cut(s)	394, 666, 850, 908, 1595, 1688
HpaI	GTTAAC	1 cut(s)	805
HpaII	CCGG	2 cut(s)	1366, 1783
Hpy188III	TCNNGA	8 cut(s)	67, 208, 663, 912, 1328, 1518, 1531, 1806
Hpy99I	CGWCG	2 cut(s)	130, 133
HpyAV	CCTTC	6 cut(s)	31, 64, 788, 852, 1130, 1277
HpyCH4III	ACNGT	4 cut(s)	196, 361, 809, 1796
HpyCH4IV	ACGT	2 cut(s)	1153, 1341
HpyF10VI	GCNNNNNNNGC	4 cut(s)	322, 542, 686, 1450
HpySE526I	ACGT	2 cut(s)	1153, 1341
Hsp92II	CATG	7 cut(s)	206, 619, 965, 993, 1048, 1528, 1534
HspAI	GCGC	2 cut(s)	278, 1418
KflI	GGGWCCC	2 cut(s)	426, 838
Ksp22I	TGATCA	1 cut(s)	285
KspAI	GTTAAC	1 cut(s)	805
KspI	CCGCGG	1 cut(s)	418
Kzo9I	GATC	8 cut(s)	158, 238, 249, 285, 742, 874, 896, 1399
LmnI	GCTCC	6 cut(s)	115, 389, 952, 1087, 1312, 1472
Lsp1109I	GCAGC	6 cut(s)	17, 131, 134, 282, 337, 1213
LweI	GCATC	2 cut(s)	522, 1431
MaeI	CTAG	4 cut(s)	476, 510, 912, 1617
MaeII	ACGT	2 cut(s)	1153, 1341
MaeIII	GTNAC	2 cut(s)	809, 1766
MalI	GATC	8 cut(s)	160, 240, 251, 287, 744, 876, 898, 1401
MbiI	CCGCTC	1 cut(s)	52
MboI	GATC	8 cut(s)	158, 238, 249, 285, 742, 874, 896, 1399
MflI	RGATCY	2 cut(s)	742, 896
MhlI	GDGCHC	2 cut(s)	1021, 1221
MluCI	AATT	9 cut(s)	11, 174, 351, 641, 766, 817, 1276, 1357, 1562
MlyI	GAGTC	2 cut(s)	388, 1682
Mph1103I	ATGCAT	1 cut(s)	719
MroXI	GAANNNNTTC	1 cut(s)	645
MseI	TTAA	7 cut(s)	222, 411, 804, 816, 1275, 1560, 1566
MslI	CAYNNNNRTG	2 cut(s)	932, 1529
MspA1I	CMGCKG	3 cut(s)	144, 417, 656
MspI	CCGG	2 cut(s)	1366, 1783
MspR9I	CCNGG	6 cut(s)	38, 446, 773, 1367, 1464, 1783
Mva1269I	GAATGC	1 cut(s)	981
MvaI	CCWGG	4 cut(s)	38, 446, 773, 1464
MvnI	CGCG	2 cut(s)	263, 417
MwoI	GCNNNNNNNGC	4 cut(s)	322, 542, 686, 1450
NciI	CCSGG	2 cut(s)	1367, 1783
NcoI	CCATGG	1 cut(s)	989
NdeII	GATC	8 cut(s)	158, 238, 249, 285, 742, 874, 896, 1399
NheI	GCTAGC	2 cut(s)	475, 509
NlaIII	CATG	7 cut(s)	206, 619, 965, 993, 1048, 1528, 1534
NmuCI	GTSAC	1 cut(s)	1766
NsiI	ATGCAT	1 cut(s)	719
NspI	RCATGY	2 cut(s)	206, 1528
PagI	TCATGA	1 cut(s)	1530
PceI	AGGCCT	1 cut(s)	444
PciI	ACATGT	1 cut(s)	202
PctI	GAATGC	1 cut(s)	981
PdmI	GAANNNNTTC	1 cut(s)	645
PfeI	GAWTC	4 cut(s)	666, 850, 908, 1595
PflMI	CCANNNNNTGG	1 cut(s)	962
PfoI	TCCNGGA	2 cut(s)	36, 1365
PkrI	GCNGC	8 cut(s)	7, 146, 149, 262, 297, 327, 335, 1228
PleI	GAGTC	2 cut(s)	388, 1682
PpsI	GAGTC	2 cut(s)	388, 1682
Ppu21I	YACGTR	1 cut(s)	1154
PpuMI	RGGWCCY	3 cut(s)	426, 838, 1193
PscI	ACATGT	1 cut(s)	202
Psp5II	RGGWCCY	3 cut(s)	426, 838, 1193
Psp6I	CCWGG	4 cut(s)	36, 444, 771, 1462
PspFI	CCCAGC	3 cut(s)	91, 1287, 1315
PspGI	CCWGG	4 cut(s)	36, 444, 771, 1462
PspPI	GGNCC	6 cut(s)	216, 426, 838, 1193, 1460, 1779
PspPPI	RGGWCCY	3 cut(s)	426, 838, 1193
PstI	CTGCAG	2 cut(s)	1231, 1555
PstNI	CAGNNNCTG	1 cut(s)	167
PsuI	RGATCY	2 cut(s)	742, 896
PvuII	CAGCTG	1 cut(s)	656
RsaI	GTAC	3 cut(s)	29, 1265, 1395
RsaNI	GTAC	3 cut(s)	28, 1264, 1394
RseI	CAYNNNNRTG	2 cut(s)	932, 1529
SacII	CCGCGG	1 cut(s)	418
SalI	GTCGAC	1 cut(s)	1302
SaqAI	TTAA	7 cut(s)	222, 411, 804, 816, 1275, 1560, 1566
SatI	GCNGC	8 cut(s)	6, 145, 148, 261, 296, 326, 334, 1227
Sau3AI	GATC	8 cut(s)	158, 238, 249, 285, 742, 874, 896, 1399
Sau96I	GGNCC	6 cut(s)	216, 426, 838, 1193, 1460, 1779
SbfI	CCTGCAGG	1 cut(s)	1555
SchI	GAGTC	2 cut(s)	388, 1682
ScrFI	CCNGG	6 cut(s)	38, 446, 773, 1367, 1464, 1783
SdaI	CCTGCAGG	1 cut(s)	1555
SduI	GDGCHC	2 cut(s)	1021, 1221
SfaNI	GCATC	2 cut(s)	522, 1431
SfcI	CTRYAG	4 cut(s)	883, 892, 1227, 1551
Sfr303I	CCGCGG	1 cut(s)	418
SgrBI	CCGCGG	1 cut(s)	418
SinI	GGWCC	4 cut(s)	426, 838, 1193, 1779
SmiMI	CAYNNNNRTG	2 cut(s)	932, 1529
Sse8387I	CCTGCAGG	1 cut(s)	1555
Sse9I	AATT	9 cut(s)	11, 174, 351, 641, 766, 817, 1276, 1357, 1562
SseBI	AGGCCT	1 cut(s)	444
SsiI	CCGC	9 cut(s)	52, 142, 261, 334, 415, 417, 1446, 1580, 1589
SspI	AATATT	1 cut(s)	1749
SspMI	CTAG	4 cut(s)	476, 510, 912, 1617
StuI	AGGCCT	1 cut(s)	444
StyD4I	CCNGG	6 cut(s)	36, 444, 771, 1365, 1462, 1781
StyI	CCWWGG	1 cut(s)	989
TaaI	ACNGT	4 cut(s)	196, 361, 809, 1796
TaiI	ACGT	2 cut(s)	1156, 1344
TaqI	TCGA	9 cut(s)	111, 209, 375, 631, 664, 792, 967, 1303, 1493
TaqII	GACCGA	1 cut(s)	186
TasI	AATT	9 cut(s)	11, 174, 351, 641, 766, 817, 1276, 1357, 1562
TatI	WGTACW	2 cut(s)	27, 1393
TauI	GCSGC	2 cut(s)	263, 336
TfiI	GAWTC	4 cut(s)	666, 850, 908, 1595
Tru1I	TTAA	7 cut(s)	222, 411, 804, 816, 1275, 1560, 1566
Tru9I	TTAA	7 cut(s)	222, 411, 804, 816, 1275, 1560, 1566
TscAI	CASTG	3 cut(s)	687, 1026, 1590
TseFI	GTSAC	1 cut(s)	1766
TseI	GCWGC	6 cut(s)	5, 144, 147, 295, 325, 1226
Tsp45I	GTSAC	1 cut(s)	1766
TspDTI	ATGAA	9 cut(s)	578, 588, 601, 638, 1149, 1227, 1365, 1519, 1547
TspGWI	ACGGA	3 cut(s)	52, 331, 1071
TspRI	CASTG	3 cut(s)	687, 1026, 1590
Van91I	CCANNNNNTGG	1 cut(s)	962
VneI	GTGCAC	2 cut(s)	1017, 1217
VpaK11BI	GGWCC	4 cut(s)	426, 838, 1193, 1779
XapI	RAATTY	3 cut(s)	641, 766, 1562
XbaI	TCTAGA	1 cut(s)	911
XceI	RCATGY	2 cut(s)	206, 1528
XmiI	GTMKAC	4 cut(s)	137, 1063, 1303, 1686
XmnI	GAANNNNTTC	1 cut(s)	645
XspI	CTAG	4 cut(s)	476, 510, 912, 1617
Zsp2I	ATGCAT	1 cut(s)	719

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.