pycom06g19140

malate transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0009313
Physical Location & Seq
Reverse (-)
22627841 .. 22629347
1507 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g19140.1

Sequence Viewer

Length: 834 bp
ATGCTTAGATATCCATGGAAGAACTATGGAAAAGTGGGTGGTGCACTGAGGCATTGTGCATTTATGGTCATGGCATTGCACGGATGTATACTTTCTGAAATACAGGCTCCTGCTGAGCGAAGACAAGTATTTCGTAGGGAACTTCAAAGGGTTGGTTATGAAGGTGCCAAAGTTTTACGTGAACTTGGAAACAAACTGAAAACAATGGAGAAAATAGGTCCCACAGATATACTCAATGAAGTGCATGAGGCTGCAGAAGACTTACAAAAGAAAGTTGACCAGAAATCGTACCTGCTTGTTAATTCAGAAGGCTGGGAGATTGGAAGTCGACCAAAGGAGCTGGGAGAACCTCAGGACTTATTGAACGTGGATGATGAAGAAAATTATTTCCGGGAATACAAATCCCTTAGTGAAGCTGTACTTGATCTTAGTTCTTTTCCTGTGCCACAAAGTTGGGATAGCCAGATGCCCGCAAAGCCAAATGGCGTAGGCTCCGTACCAACTGAAGTCAACCACTCGAACCTGCCCGCAGGTGTTTCATCAGGAAGTATGTTCATGAAACAGATATCGTGGCCTGCAGGACTTAAATTTAAGGCTGATGAACCGCCAGTAGCGGAGGAATCCAAAACCTATGAAAATGCTAGTCAGTTGTCATTAGCCACATTTACATCCCTGTTGATAGAGTTTGTCGCAAGGCTTCAAAATCTTGTAGACTCATTTCAAGAACTAAGTGAGAAAGCACATTTCAAGGAACCTGCCGAACCACCAGAAATCTTAGAGCCACCTCGCGGTCGCAGGTTTTGGACCCGATTGCTCAACTGTCTGAAATCCTGA

Protein Analysis

278

Amino Acids

31.28

Weight (kDa)

5.7

Isoelectric Point (pI)

54.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ALMT PF11744 2 - 244 6.4e-56 Aluminium activated malate transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 521
Acc36I ACCTGC 5 cut(s) 300, 521, 531, 763, 786
AccB1I GGYRCC 1 cut(s) 164
AccI GTMKAC 3 cut(s) 88, 328, 711
AccII CGCG 1 cut(s) 789
AciI CCGC 5 cut(s) 471, 528, 605, 614, 789
AcsI RAATTY 1 cut(s) 587
AcuI CTGAAG 1 cut(s) 525
AfaI GTAC 3 cut(s) 290, 420, 498
AfiI CCNNNNNNNGG 1 cut(s) 788
AgsI TTSAA 5 cut(s) 146, 364, 701, 722, 748
AluBI AGCT 2 cut(s) 340, 416
AluI AGCT 2 cut(s) 340, 416
Alw21I GWGCWC 1 cut(s) 46
Alw44I GTGCAC 1 cut(s) 42
AoxI GGCC 1 cut(s) 572
ApaLI GTGCAC 1 cut(s) 42
ApeKI GCWGC 1 cut(s) 251
ApoI RAATTY 1 cut(s) 587
ArsI GACNNNNNNTTYG 2 cut(s) 114, 146
AspS9I GGNCC 2 cut(s) 218, 804
AsuC2I CCSGG 1 cut(s) 392
AvaII GGWCC 2 cut(s) 218, 804
AxyI CCTNAGG 1 cut(s) 351
BaeGI GKGCMC 1 cut(s) 46
BanI GGYRCC 1 cut(s) 164
BbsI GAAGAC 2 cut(s) 127, 264
Bbv12I GWGCWC 1 cut(s) 46
BbvI GCAGC 1 cut(s) 238
BcnI CCSGG 1 cut(s) 392
BfaI CTAG 1 cut(s) 642
BfmI CTRYAG 2 cut(s) 252, 576
BfuAI ACCTGC 5 cut(s) 300, 521, 531, 763, 786
BisI GCNGC 1 cut(s) 252
BlpI GCTNAGC 1 cut(s) 114
BlsI GCNGC 1 cut(s) 253
Bme1390I CCNGG 1 cut(s) 392
Bme18I GGWCC 2 cut(s) 218, 804
BmgT120I GGNCC 2 cut(s) 218, 804
BmiI GGNNCC 6 cut(s) 108, 166, 220, 493, 753, 806
BmrFI CCNGG 1 cut(s) 392
BmsI GCATC 1 cut(s) 456
BpiI GAAGAC 2 cut(s) 127, 264
Bpu1102I GCTNAGC 1 cut(s) 114
BpuMI CCSGG 1 cut(s) 392
BsaAI YACGTR 1 cut(s) 179
BsaJI CCNNGG 1 cut(s) 14
Bsc4I CCNNNNNNNGG 1 cut(s) 788
Bse1I ACTGG 1 cut(s) 608
Bse21I CCTNAGG 1 cut(s) 351
Bse3DI GCAATG 1 cut(s) 74
BseDI CCNNGG 1 cut(s) 14
BseGI GGATG 3 cut(s) 89, 376, 668
BseLI CCNNNNNNNGG 1 cut(s) 788
BseMI GCAATG 1 cut(s) 74
BseMII CTCAG 3 cut(s) 38, 105, 365
BseNI ACTGG 1 cut(s) 608
BseSI GKGCMC 1 cut(s) 46
BseXI GCAGC 1 cut(s) 238
BseYI CCCAGC 2 cut(s) 312, 340
Bsh1236I CGCG 1 cut(s) 789
Bsh1285I CGRYCG 1 cut(s) 793
BshFI GGCC 1 cut(s) 574
BshNI GGYRCC 1 cut(s) 164
BsiEI CGRYCG 1 cut(s) 793
BsiHKAI GWGCWC 1 cut(s) 46
BsiSI CCGG 1 cut(s) 391
BslFI GGGAC 1 cut(s) 204
BslI CCNNNNNNNGG 1 cut(s) 788
BsmFI GGGAC 1 cut(s) 204
BsnI GGCC 1 cut(s) 574
Bsp1286I GDGCHC 1 cut(s) 46
Bsp143I GATC 1 cut(s) 424
Bsp1720I GCTNAGC 1 cut(s) 114
Bsp19I CCATGG 1 cut(s) 14
BspACI CCGC 5 cut(s) 471, 528, 605, 614, 789
BspANI GGCC 1 cut(s) 574
BspCNI CTCAG 3 cut(s) 39, 106, 364
BspFNI CGCG 1 cut(s) 789
BspHI TCATGA 1 cut(s) 555
BspLI GGNNCC 6 cut(s) 108, 166, 220, 493, 753, 806
BspMAI CTGCAG 2 cut(s) 256, 580
BspMI ACCTGC 5 cut(s) 300, 521, 531, 763, 786
BspT107I GGYRCC 1 cut(s) 164
BsrDI GCAATG 1 cut(s) 74
BsrI ACTGG 1 cut(s) 608
BssECI CCNNGG 1 cut(s) 14
BssMI GATC 1 cut(s) 424
BssNAI GTATAC 1 cut(s) 89
BssT1I CCWWGG 1 cut(s) 14
Bst1107I GTATAC 1 cut(s) 89
Bst4CI ACNGT 1 cut(s) 821
BstBAI YACGTR 1 cut(s) 179
BstC8I GCNNGC 3 cut(s) 471, 528, 576
BstDEI CTNAG 8 cut(s) 5, 47, 114, 351, 407, 428, 728, 775
BstDSI CCRYGG 1 cut(s) 14
BstF5I GGATG 3 cut(s) 89, 376, 668
BstFNI CGCG 1 cut(s) 789
BstKTI GATC 1 cut(s) 427
BstMBI GATC 1 cut(s) 424
BstMCI CGRYCG 1 cut(s) 793
BstMWI GCNNNNNNNGC 1 cut(s) 475
BstSCI CCNGG 1 cut(s) 390
BstSFI CTRYAG 2 cut(s) 252, 576
BstSLI GKGCMC 1 cut(s) 46
BstUI CGCG 1 cut(s) 789
BstV1I GCAGC 1 cut(s) 238
BstV2I GAAGAC 2 cut(s) 127, 264
BstXI CCANNNNNNTGG 1 cut(s) 453
BstZ17I GTATAC 1 cut(s) 89
Bsu36I CCTNAGG 1 cut(s) 351
BsuRI GGCC 1 cut(s) 574
BtgI CCRYGG 1 cut(s) 14
BtsCI GGATG 3 cut(s) 89, 376, 668
BtsIMutI CAGTG 1 cut(s) 44
BveI ACCTGC 5 cut(s) 300, 521, 531, 763, 786
Cac8I GCNNGC 3 cut(s) 471, 528, 576
CciI TCATGA 1 cut(s) 555
Cfr13I GGNCC 2 cut(s) 218, 804
Csp6I GTAC 3 cut(s) 289, 419, 497
CviAII CATG 4 cut(s) 15, 70, 245, 556
CviQI GTAC 3 cut(s) 289, 419, 497
DdeI CTNAG 8 cut(s) 5, 47, 114, 351, 407, 428, 728, 775
DpnI GATC 1 cut(s) 426
DpnII GATC 1 cut(s) 424
Eco130I CCWWGG 1 cut(s) 14
Eco32I GATATC 2 cut(s) 11, 567
Eco47I GGWCC 2 cut(s) 218, 804
Eco57I CTGAAG 1 cut(s) 525
Eco81I CCTNAGG 1 cut(s) 351
EcoO109I RGGNCCY 1 cut(s) 218
EcoRV GATATC 2 cut(s) 11, 567
EcoT14I CCWWGG 1 cut(s) 14
ErhI CCWWGG 1 cut(s) 14
FaeI CATG 4 cut(s) 18, 73, 248, 559
FalI AAGNNNNNCTT 2 cut(s) 405, 437
FaqI GGGAC 1 cut(s) 204
FatI CATG 4 cut(s) 14, 69, 244, 555
FauI CCCGC 2 cut(s) 478, 535
FblI GTMKAC 3 cut(s) 88, 328, 711
Fnu4HI GCNGC 1 cut(s) 252
FokI GGATG 3 cut(s) 96, 383, 655
Fsp4HI GCNGC 1 cut(s) 252
FspBI CTAG 1 cut(s) 642
GluI GCNGC 1 cut(s) 252
GsaI CCCAGC 2 cut(s) 316, 344
HaeIII GGCC 1 cut(s) 574
HapII CCGG 1 cut(s) 391
Hin1II CATG 4 cut(s) 18, 73, 248, 559
HincII GTYRAC 3 cut(s) 277, 329, 511
HindII GTYRAC 3 cut(s) 277, 329, 511
HinfI GANTC 2 cut(s) 620, 713
HpaII CCGG 1 cut(s) 391
Hpy166II GTNNAC 7 cut(s) 44, 89, 182, 277, 329, 511, 712
Hpy188I TCNGA 3 cut(s) 97, 307, 825
Hpy188III TCNNGA 5 cut(s) 353, 543, 556, 722, 831
Hpy8I GTNNAC 7 cut(s) 44, 89, 182, 277, 329, 511, 712
HpyAV CCTTC 2 cut(s) 155, 302
HpyCH4III ACNGT 1 cut(s) 821
HpyCH4IV ACGT 2 cut(s) 178, 366
HpyCH4V TGCA 6 cut(s) 44, 59, 79, 244, 254, 578
HpyF10VI GCNNNNNNNGC 1 cut(s) 475
HpyF3I CTNAG 8 cut(s) 5, 47, 114, 351, 407, 428, 728, 775
HpySE526I ACGT 2 cut(s) 178, 366
Hsp92II CATG 4 cut(s) 18, 73, 248, 559
Kzo9I GATC 1 cut(s) 424
LmnI GCTCC 3 cut(s) 112, 337, 497
Lsp1109I GCAGC 1 cut(s) 238
LweI GCATC 1 cut(s) 456
MaeI CTAG 1 cut(s) 642
MaeII ACGT 2 cut(s) 178, 366
MalI GATC 1 cut(s) 426
MboI GATC 1 cut(s) 424
MboII GAAGA 4 cut(s) 31, 132, 269, 389
MhlI GDGCHC 1 cut(s) 46
MluCI AATT 3 cut(s) 301, 382, 587
MlyI GAGTC 1 cut(s) 707
MnlI CCTC 5 cut(s) 42, 241, 360, 610, 795
MseI TTAA 3 cut(s) 300, 585, 591
MspI CCGG 1 cut(s) 391
MspR9I CCNGG 1 cut(s) 392
MvnI CGCG 1 cut(s) 789
MwoI GCNNNNNNNGC 1 cut(s) 475
NciI CCSGG 1 cut(s) 392
NcoI CCATGG 1 cut(s) 14
NdeII GATC 1 cut(s) 424
NlaIII CATG 4 cut(s) 18, 73, 248, 559
NlaIV GGNNCC 6 cut(s) 108, 166, 220, 493, 753, 806
PagI TCATGA 1 cut(s) 555
PaqCI CACCTGC 1 cut(s) 521
PfeI GAWTC 1 cut(s) 620
PfoI TCCNGGA 1 cut(s) 390
PkrI GCNGC 1 cut(s) 253
PleI GAGTC 1 cut(s) 707
PpsI GAGTC 1 cut(s) 707
Ppu21I YACGTR 1 cut(s) 179
PpuMI RGGWCCY 1 cut(s) 218
Psp5II RGGWCCY 1 cut(s) 218
PspFI CCCAGC 2 cut(s) 312, 340
PspN4I GGNNCC 6 cut(s) 108, 166, 220, 493, 753, 806
PspPI GGNCC 2 cut(s) 218, 804
PspPPI RGGWCCY 1 cut(s) 218
PstI CTGCAG 2 cut(s) 256, 580
RsaI GTAC 3 cut(s) 290, 420, 498
RsaNI GTAC 3 cut(s) 289, 419, 497
SalI GTCGAC 1 cut(s) 327
SaqAI TTAA 3 cut(s) 300, 585, 591
SatI GCNGC 1 cut(s) 252
Sau3AI GATC 1 cut(s) 424
Sau96I GGNCC 2 cut(s) 218, 804
SbfI CCTGCAGG 1 cut(s) 580
SchI GAGTC 1 cut(s) 707
ScrFI CCNGG 1 cut(s) 392
SdaI CCTGCAGG 1 cut(s) 580
SduI GDGCHC 1 cut(s) 46
SfaNI GCATC 1 cut(s) 456
SfcI CTRYAG 2 cut(s) 252, 576
SinI GGWCC 2 cut(s) 218, 804
Sse8387I CCTGCAGG 1 cut(s) 580
Sse9I AATT 3 cut(s) 301, 382, 587
SsiI CCGC 5 cut(s) 471, 528, 605, 614, 789
SspMI CTAG 1 cut(s) 642
StyD4I CCNGG 1 cut(s) 390
StyI CCWWGG 1 cut(s) 14
TaaI ACNGT 1 cut(s) 821
TaiI ACGT 2 cut(s) 181, 369
TaqI TCGA 2 cut(s) 328, 518
TasI AATT 3 cut(s) 301, 382, 587
TatI WGTACW 1 cut(s) 418
TfiI GAWTC 1 cut(s) 620
Tru1I TTAA 3 cut(s) 300, 585, 591
Tru9I TTAA 3 cut(s) 300, 585, 591
TscAI CASTG 1 cut(s) 51
TseI GCWGC 1 cut(s) 251
TspDTI ATGAA 8 cut(s) 174, 252, 390, 528, 544, 572, 615, 648
TspGWI ACGGA 2 cut(s) 96, 484
TspRI CASTG 1 cut(s) 51
VneI GTGCAC 1 cut(s) 42
VpaK11BI GGWCC 2 cut(s) 218, 804
XapI RAATTY 1 cut(s) 587
XmiI GTMKAC 3 cut(s) 88, 328, 711
XspI CTAG 1 cut(s) 642
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.