MD06G1215300.v1.1

protein kinase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0016335
Physical Location & Seq
Reverse (-)
34755362 .. 34756102
741 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1215300.v1.1.491

Sequence Viewer

Length: 741 bp
ATGGATGACACAAGCAACCACAATAGGAAGCGAGCCCGAGATGACTCAGACAACTCGGAGAACTCAGAAACCAATAGCACCCAACCCGATACAAAACTCATCCGAGTCGACTCGAGCAACTCGGTTGCCAGCTCCCACCAGTCGGGCTACACCGGAGATGAATTGAACAACTCGGATATTAACTCGGGCGAGCCATCGACTCAGACTCCCGATCGAGTTGACTCGGACGAGTTGCGTATGGGGTCGGCGGAGGTCAAGCTGATACAAGACGACCTACTTAACATCCTGGACGACTCGGACGCCGTCCCGGAGATTCAAGACCTTGACTCGGTGATTAAGAGCTTCGAGGAGGAGATACAAGTTCCGGCATTTCCCGCGTCCGAAGACACGTCGGCTTCAGGCGAGTCGAGGCAGCCAGAGCTTGGTTACCTTCTGGAAGCTTCGGACGACGAGCTTGGTCTCCCACCGACCAATGGAGCAAATGAAGAGGAAAAAATCGAGGCCGCTGATTTCACCGAGAGTGGTTCCCAGGCCTTTGGATTTGATGGCATGGTAGGGTTCGATAACGACTTGATTCCAAGTTACGACTCGTTCGATTTCGGAATCGGTGGCAACTCTGAATTTATTAATGACAGTTATATTGGCGGGGAGGAGTACTTGGCGTTAGGCGGTTTGTTTGATTACTCCGGCGGCGGCGTGTCGGATGATTCGTGGCGGACCGAGTCGCTTTCCGCTTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

247

Amino Acids

26.49

Weight (kDa)

4.05

Isoelectric Point (pI)

50.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016335)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g12280
malus_domestica MD06G1215300.v1.1 MD14G1226200.v1.1
prunus_persica Prupe.5G220600_v2.0.a1
pyrus_communis pycom06g19200
rosa_chinensis RchiOBHm_Chr7g0185281
rosa_laevigata RLG00000004925
rosa_multiflora Rmu_sc0008496.1_g000015
rosa_roxburghii Rroxscaffold_3G00269090
rosa_rugosa Rorug06G0467500
rosa_samantha Rh7AG075200 Rh7BG072000 Rh7CG073200 Rh7DG072700
rosa_wichuraiana Rw7G006050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 422
AccI GTMKAC 1 cut(s) 108
AccII CGCG 1 cut(s) 377
AciI CCGC 9 cut(s) 248, 375, 504, 645, 669, 690, 693, 715, 732
AcsI RAATTY 1 cut(s) 620
AcuI CTGAAG 1 cut(s) 381
AcyI GRCGYC 1 cut(s) 300
AfaI GTAC 1 cut(s) 656
AfiI CCNNNNNNNGG 4 cut(s) 142, 328, 422, 473
AflIII ACRYGT 1 cut(s) 387
AgsI TTSAA 2 cut(s) 166, 317
AjiI CACGTC 1 cut(s) 390
AjnI CCWGG 2 cut(s) 285, 528
AluBI AGCT 6 cut(s) 132, 259, 342, 421, 440, 454
AluI AGCT 6 cut(s) 132, 259, 342, 421, 440, 454
Alw26I GTCTC 1 cut(s) 464
Ama87I CYCGRG 3 cut(s) 36, 112, 184
AoxI GGCC 2 cut(s) 501, 531
ApeKI GCWGC 1 cut(s) 412
ApoI RAATTY 1 cut(s) 620
AseI ATTAAT 1 cut(s) 627
AspS9I GGNCC 1 cut(s) 717
AsuC2I CCSGG 1 cut(s) 308
AsuHPI GGTGA 2 cut(s) 343, 505
AvaI CYCGRG 3 cut(s) 36, 112, 184
AvaII GGWCC 1 cut(s) 717
BanII GRGCYC 1 cut(s) 37
BbsI GAAGAC 1 cut(s) 390
BbvI GCAGC 1 cut(s) 424
BccI CCATC 2 cut(s) 202, 539
BceAI ACGGC 1 cut(s) 287
BciT130I CCWGG 2 cut(s) 287, 530
BcnI CCSGG 1 cut(s) 308
BcoDI GTCTC 1 cut(s) 464
BisI GCNGC 4 cut(s) 413, 504, 691, 694
BlsI GCNGC 4 cut(s) 414, 505, 692, 695
BmcAI AGTACT 1 cut(s) 656
Bme1390I CCNGG 3 cut(s) 287, 308, 530
Bme18I GGWCC 1 cut(s) 717
BmeT110I CYCGRG 3 cut(s) 36, 112, 184
BmgBI CACGTC 1 cut(s) 390
BmgT120I GGNCC 1 cut(s) 717
BmiI GGNNCC 1 cut(s) 526
BmrFI CCNGG 3 cut(s) 287, 308, 530
BpiI GAAGAC 1 cut(s) 390
BpuMI CCSGG 1 cut(s) 308
BsaHI GRCGYC 1 cut(s) 300
BsaI GGTCTC 1 cut(s) 464
BsaJI CCNNGG 1 cut(s) 528
BsaWI WCCGGW 1 cut(s) 152
BsaXI ACNNNNNCTCC 2 cut(s) 190, 220
Bsc4I CCNNNNNNNGG 4 cut(s) 142, 328, 422, 473
Bse1I ACTGG 1 cut(s) 139
BseBI CCWGG 2 cut(s) 287, 530
BseDI CCNNGG 1 cut(s) 528
BseGI GGATG 4 cut(s) 10, 99, 282, 709
BseLI CCNNNNNNNGG 4 cut(s) 142, 328, 422, 473
BseMII CTCAG 3 cut(s) 60, 78, 215
BseNI ACTGG 1 cut(s) 139
BseRI GAGGAG 3 cut(s) 362, 365, 665
BseXI GCAGC 1 cut(s) 424
Bsh1236I CGCG 1 cut(s) 377
Bsh1285I CGRYCG 1 cut(s) 214
BshFI GGCC 2 cut(s) 503, 533
BsiEI CGRYCG 1 cut(s) 214
BsiHKCI CYCGRG 3 cut(s) 36, 112, 184
BsiSI CCGG 4 cut(s) 153, 308, 365, 687
BslFI GGGAC 1 cut(s) 290
BslI CCNNNNNNNGG 4 cut(s) 142, 328, 422, 473
BsmAI GTCTC 1 cut(s) 464
BsmFI GGGAC 1 cut(s) 290
BsnI GGCC 2 cut(s) 503, 533
Bso31I GGTCTC 1 cut(s) 464
BsoBI CYCGRG 3 cut(s) 36, 112, 184
Bsp1286I GDGCHC 1 cut(s) 37
Bsp143I GATC 1 cut(s) 211
BspACI CCGC 9 cut(s) 248, 375, 504, 645, 669, 690, 693, 715, 732
BspANI GGCC 2 cut(s) 503, 533
BspCNI CTCAG 3 cut(s) 59, 77, 214
BspFNI CGCG 1 cut(s) 377
BspLI GGNNCC 1 cut(s) 526
BspTNI GGTCTC 1 cut(s) 464
BsrI ACTGG 1 cut(s) 139
BssECI CCNNGG 1 cut(s) 528
BssMI GATC 1 cut(s) 211
BssNI GRCGYC 1 cut(s) 300
Bst2UI CCWGG 2 cut(s) 287, 530
Bst4CI ACNGT 1 cut(s) 635
Bst6I CTCTTC 1 cut(s) 480
BstACI GRCGYC 1 cut(s) 300
BstC8I GCNNGC 3 cut(s) 33, 130, 191
BstDEI CTNAG 3 cut(s) 46, 64, 201
BstEII GGTNACC 1 cut(s) 425
BstF5I GGATG 4 cut(s) 10, 99, 282, 709
BstFNI CGCG 1 cut(s) 377
BstKTI GATC 1 cut(s) 214
BstMAI GTCTC 1 cut(s) 464
BstMBI GATC 1 cut(s) 211
BstMCI CGRYCG 1 cut(s) 214
BstMWI GCNNNNNNNGC 2 cut(s) 374, 418
BstNI CCWGG 2 cut(s) 287, 530
BstPI GGTNACC 1 cut(s) 425
BstSCI CCNGG 3 cut(s) 285, 306, 528
BstUI CGCG 1 cut(s) 377
BstV1I GCAGC 1 cut(s) 424
BstV2I GAAGAC 1 cut(s) 390
BstXI CCANNNNNNTGG 1 cut(s) 536
BsuRI GGCC 2 cut(s) 503, 533
BtrI CACGTC 1 cut(s) 390
BtsCI GGATG 4 cut(s) 10, 99, 282, 709
Cac8I GCNNGC 3 cut(s) 33, 130, 191
Cfr13I GGNCC 1 cut(s) 717
CpoI CGGWCCG 1 cut(s) 717
CseI GACGC 2 cut(s) 308, 366
Csp6I GTAC 1 cut(s) 655
CspI CGGWCCG 1 cut(s) 717
CviAII CATG 1 cut(s) 550
CviQI GTAC 1 cut(s) 655
DdeI CTNAG 3 cut(s) 46, 64, 201
DpnI GATC 1 cut(s) 213
DpnII GATC 1 cut(s) 211
Eam1104I CTCTTC 1 cut(s) 480
EarI CTCTTC 1 cut(s) 480
EciI GGCGGA 2 cut(s) 263, 730
Eco147I AGGCCT 1 cut(s) 533
Eco24I GRGCYC 1 cut(s) 37
Eco31I GGTCTC 1 cut(s) 464
Eco47I GGWCC 1 cut(s) 717
Eco57I CTGAAG 1 cut(s) 381
Eco88I CYCGRG 3 cut(s) 36, 112, 184
Eco91I GGTNACC 1 cut(s) 425
EcoO65I GGTNACC 1 cut(s) 425
EcoRII CCWGG 2 cut(s) 285, 528
EcoT38I GRGCYC 1 cut(s) 37
FaeI CATG 1 cut(s) 553
FaiI YATR 3 cut(s) 239, 551, 639
FaqI GGGAC 1 cut(s) 290
FatI CATG 1 cut(s) 549
FauI CCCGC 2 cut(s) 382, 638
FblI GTMKAC 1 cut(s) 108
Fnu4HI GCNGC 4 cut(s) 413, 504, 691, 694
FokI GGATG 4 cut(s) 17, 86, 269, 716
FriOI GRGCYC 1 cut(s) 37
Fsp4HI GCNGC 4 cut(s) 413, 504, 691, 694
GluI GCNGC 4 cut(s) 413, 504, 691, 694
HaeIII GGCC 2 cut(s) 503, 533
HapII CCGG 4 cut(s) 153, 308, 365, 687
HgaI GACGC 2 cut(s) 308, 366
Hin1I GRCGYC 1 cut(s) 300
Hin1II CATG 1 cut(s) 553
HincII GTYRAC 2 cut(s) 109, 220
HindII GTYRAC 2 cut(s) 109, 220
HindIII AAGCTT 1 cut(s) 438
HpaII CCGG 4 cut(s) 153, 308, 365, 687
HphI GGTGA 2 cut(s) 343, 505
Hpy166II GTNNAC 2 cut(s) 109, 220
Hpy188III TCNNGA 3 cut(s) 209, 317, 434
Hpy8I GTNNAC 2 cut(s) 109, 220
Hpy99I CGWCG 2 cut(s) 394, 452
HpyAV CCTTC 1 cut(s) 440
HpyCH4III ACNGT 1 cut(s) 635
HpyCH4IV ACGT 1 cut(s) 389
HpyF10VI GCNNNNNNNGC 2 cut(s) 374, 418
HpyF3I CTNAG 3 cut(s) 46, 64, 201
HpySE526I ACGT 1 cut(s) 389
Hsp92I GRCGYC 1 cut(s) 300
Hsp92II CATG 1 cut(s) 553
Kzo9I GATC 1 cut(s) 211
LmnI GCTCC 2 cut(s) 137, 476
Lsp1109I GCAGC 1 cut(s) 424
MaeII ACGT 1 cut(s) 389
MaeIII GTNAC 2 cut(s) 425, 581
MalI GATC 1 cut(s) 213
MboI GATC 1 cut(s) 211
MboII GAAGA 2 cut(s) 395, 497
MhlI GDGCHC 1 cut(s) 37
MluCI AATT 2 cut(s) 161, 620
MmeI TCCRAC 1 cut(s) 681
MnlI CCTC 7 cut(s) 244, 340, 343, 402, 481, 493, 643
MseI TTAA 4 cut(s) 180, 279, 336, 627
MspA1I CMGCKG 1 cut(s) 506
MspI CCGG 4 cut(s) 153, 308, 365, 687
MspR9I CCNGG 3 cut(s) 287, 308, 530
MvaI CCWGG 2 cut(s) 287, 530
MvnI CGCG 1 cut(s) 377
MwoI GCNNNNNNNGC 2 cut(s) 374, 418
NciI CCSGG 1 cut(s) 308
NdeII GATC 1 cut(s) 211
NlaIII CATG 1 cut(s) 553
NlaIV GGNNCC 1 cut(s) 526
PaeR7I CTCGAG 1 cut(s) 112
PceI AGGCCT 1 cut(s) 533
PcsI WCGNNNNNNNCGW 2 cut(s) 591, 707
PfeI GAWTC 4 cut(s) 313, 574, 603, 707
PflFI GACNNNGTC 2 cut(s) 302, 721
PflMI CCANNNNNTGG 1 cut(s) 422
PfoI TCCNGGA 2 cut(s) 285, 306
PkrI GCNGC 4 cut(s) 414, 505, 692, 695
Ple19I CGATCG 1 cut(s) 214
PshBI ATTAAT 1 cut(s) 627
Psp6I CCWGG 2 cut(s) 285, 528
PspEI GGTNACC 1 cut(s) 425
PspGI CCWGG 2 cut(s) 285, 528
PspN4I GGNNCC 1 cut(s) 526
PspPI GGNCC 1 cut(s) 717
PspXI VCTCGAGB 1 cut(s) 112
PsyI GACNNNGTC 2 cut(s) 302, 721
PvuI CGATCG 1 cut(s) 214
RsaI GTAC 1 cut(s) 656
RsaNI GTAC 1 cut(s) 655
Rsr2I CGGWCCG 1 cut(s) 717
RsrII CGGWCCG 1 cut(s) 717
SalI GTCGAC 1 cut(s) 107
SaqAI TTAA 4 cut(s) 180, 279, 336, 627
SatI GCNGC 4 cut(s) 413, 504, 691, 694
Sau3AI GATC 1 cut(s) 211
Sau96I GGNCC 1 cut(s) 717
ScaI AGTACT 1 cut(s) 656
ScrFI CCNGG 3 cut(s) 287, 308, 530
SduI GDGCHC 1 cut(s) 37
Sfr274I CTCGAG 1 cut(s) 112
SinI GGWCC 1 cut(s) 717
SlaI CTCGAG 1 cut(s) 112
SmlI CTYRAG 1 cut(s) 112
SmoI CTYRAG 1 cut(s) 112
Sse9I AATT 2 cut(s) 161, 620
SseBI AGGCCT 1 cut(s) 533
SsiI CCGC 9 cut(s) 248, 375, 504, 645, 669, 690, 693, 715, 732
StuI AGGCCT 1 cut(s) 533
StyD4I CCNGG 3 cut(s) 285, 306, 528
TaaI ACNGT 1 cut(s) 635
TaiI ACGT 1 cut(s) 392
TaqI TCGA 9 cut(s) 108, 113, 197, 214, 345, 407, 498, 561, 594
TaqII GACCGA 1 cut(s) 734
TasI AATT 2 cut(s) 161, 620
TatI WGTACW 1 cut(s) 654
TauI GCSGC 3 cut(s) 506, 693, 696
TfiI GAWTC 4 cut(s) 313, 574, 603, 707
Tru1I TTAA 4 cut(s) 180, 279, 336, 627
Tru9I TTAA 4 cut(s) 180, 279, 336, 627
TseI GCWGC 1 cut(s) 412
TspDTI ATGAA 2 cut(s) 174, 498
Tth111I GACNNNGTC 2 cut(s) 302, 721
Van91I CCANNNNNTGG 1 cut(s) 422
VpaK11BI GGWCC 1 cut(s) 717
VspI ATTAAT 1 cut(s) 627
XapI RAATTY 1 cut(s) 620
XhoI CTCGAG 1 cut(s) 112
XmiI GTMKAC 1 cut(s) 108
ZrmI AGTACT 1 cut(s) 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.