MD06G1222100.v1.1

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0015034
Physical Location & Seq
Reverse (-)
35374724 .. 35377057
2334 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1222100.v1.1.491

Sequence Viewer

Length: 708 bp
ATGGAAGCGGAGCAGGTGGACTATGTGCTAGTGCCGCTGGGTCTGCTGGTGCTGGGTGCATACCACGTATGGCTGCTGATCACCATCCACCGCCATCCTATTCGAACCGTCGTCGGCCTCAACGCTCAGTCTCGCCACCAATGGGTCTTATCCATCATGTCTGACCCCTTGAAGAATGGTGTTTTGGCTGTTCAAACGATACGCAACAACATAATGGCGTCTACGCTGTTGGCAACAACAGCCATCACTCTCAGTTCTCTCATCGGCGTATTTGTCAGCACTACATCAGACTCCGGCAACACAGCATCCAGATTAATTTATGGGAACAAGAGTCCGCTTCTCTCTTCAGTCAAGTACTTTTCCATCTTGCTTTGCTTTCTCGTTGCCTTTCTCTTGAATGTGCAGTCTATTAGATACTATGCACATGTTAGCTTCTTAGCCACTGTGCCCACATGGAAAGGCCAGAAGGATTACCTTGAGTATGTTGCGCGAAACTTGAATCGCGGAAGCTATTTTTGGTCCCTCGGATTGCGAGCATTCTACCTCTCGTTCCCTCTCTTCCTCTGGATATTTGGTCCCATCCCCATGTTTGTGTGCTGTTGCATTATGTTATTTGTTCTGTATTTCTTGGACACAACCACCAGTTTTACTAGACATCTTCACACTAATTCCATCCGAAGAACTGATGATGTCGAATCAGTTGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

236

Amino Acids

26.54

Weight (kDa)

9.25

Isoelectric Point (pI)

32.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 9 - 214 2.1e-72 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015034)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18215 AT3G18215
fragaria_vesca FvH4_5g16810
malus_domestica MD06G1222100.v1.1 MD15G1311900.v1.1
prunus_persica Prupe.5G226500_v2.0.a1 Prupe.5G226500_v2.0.a1
pyrus_communis pycom06g19760
rosa_chinensis RchiOBHm_Chr7g0179261
rosa_laevigata RLG00000005368
rosa_multiflora Rmu_sc0006939.1_g000006
rosa_roxburghii Rroxscaffold_3G00274170
rosa_rugosa Rorug06G0422800
rosa_samantha Rh7CG023300 Rh7DG022600
rosa_wichuraiana Rw7G001820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 4
Acc36I ACCTGC 1 cut(s) 4
AccI GTMKAC 1 cut(s) 221
AccII CGCG 2 cut(s) 490, 504
AciI CCGC 5 cut(s) 8, 35, 91, 335, 504
AcuI CTGAAG 1 cut(s) 330
AcyI GRCGYC 1 cut(s) 218
AfaI GTAC 1 cut(s) 356
AfiI CCNNNNNNNGG 1 cut(s) 142
AflIII ACRYGT 1 cut(s) 424
AgsI TTSAA 4 cut(s) 172, 194, 397, 499
AjuI GAANNNNNNNTTGG 4 cut(s) 167, 199, 499, 531
AluBI AGCT 2 cut(s) 432, 510
AluI AGCT 2 cut(s) 432, 510
Alw26I GTCTC 1 cut(s) 135
AoxI GGCC 2 cut(s) 115, 460
ApeKI GCWGC 1 cut(s) 73
AseI ATTAAT 1 cut(s) 314
AspLEI GCGC 1 cut(s) 490
AspS9I GGNCC 2 cut(s) 519, 575
AsuHPI GGTGA 1 cut(s) 73
AsuII TTCGAA 1 cut(s) 103
AvaII GGWCC 2 cut(s) 519, 575
BaeGI GKGCMC 1 cut(s) 450
BbvI GCAGC 1 cut(s) 60
BccI CCATC 7 cut(s) 92, 102, 161, 251, 371, 587, 680
BclI TGATCA 1 cut(s) 78
BcoDI GTCTC 1 cut(s) 135
BfaI CTAG 2 cut(s) 29, 651
BfuAI ACCTGC 1 cut(s) 4
BisI GCNGC 2 cut(s) 35, 74
BlsI GCNGC 2 cut(s) 36, 75
BmcAI AGTACT 1 cut(s) 356
Bme18I GGWCC 2 cut(s) 519, 575
BmgT120I GGNCC 2 cut(s) 519, 575
BmiI GGNNCC 2 cut(s) 521, 577
BmsI GCATC 1 cut(s) 314
Bpu14I TTCGAA 1 cut(s) 103
BpuEI CTTGAG 1 cut(s) 497
BsaAI YACGTR 1 cut(s) 67
BsaBI GATNNNNATC 1 cut(s) 83
BsaHI GRCGYC 1 cut(s) 218
BsaJI CCNNGG 1 cut(s) 523
Bsc4I CCNNNNNNNGG 1 cut(s) 142
Bse1I ACTGG 1 cut(s) 642
Bse8I GATNNNNATC 1 cut(s) 83
BseDI CCNNGG 1 cut(s) 523
BseGI GGATG 5 cut(s) 84, 94, 305, 579, 672
BseJI GATNNNNATC 1 cut(s) 83
BseLI CCNNNNNNNGG 1 cut(s) 142
BseMII CTCAG 2 cut(s) 140, 265
BseNI ACTGG 1 cut(s) 642
BseSI GKGCMC 1 cut(s) 450
BseXI GCAGC 1 cut(s) 60
BseYI CCCAGC 2 cut(s) 37, 52
BsgI GTGCAG 1 cut(s) 422
Bsh1236I CGCG 2 cut(s) 490, 504
BshFI GGCC 2 cut(s) 117, 462
BsiSI CCGG 1 cut(s) 294
BslFI GGGAC 2 cut(s) 505, 561
BslI CCNNNNNNNGG 1 cut(s) 142
BsmAI GTCTC 1 cut(s) 135
BsmFI GGGAC 2 cut(s) 505, 561
BsmI GAATGC 1 cut(s) 536
BsnI GGCC 2 cut(s) 117, 462
Bsp119I TTCGAA 1 cut(s) 103
Bsp1286I GDGCHC 1 cut(s) 450
Bsp143I GATC 1 cut(s) 78
BspACI CCGC 5 cut(s) 8, 35, 91, 335, 504
BspANI GGCC 2 cut(s) 117, 462
BspCNI CTCAG 2 cut(s) 139, 264
BspFNI CGCG 2 cut(s) 490, 504
BspLI GGNNCC 2 cut(s) 521, 577
BspMI ACCTGC 1 cut(s) 4
BspT104I TTCGAA 1 cut(s) 103
BsrI ACTGG 1 cut(s) 642
BssECI CCNNGG 1 cut(s) 523
BssMI GATC 1 cut(s) 78
BssNI GRCGYC 1 cut(s) 218
Bst4CI ACNGT 2 cut(s) 109, 445
Bst6I CTCTTC 2 cut(s) 349, 563
BstACI GRCGYC 1 cut(s) 218
BstBAI YACGTR 1 cut(s) 67
BstBI TTCGAA 1 cut(s) 103
BstC8I GCNNGC 1 cut(s) 534
BstDEI CTNAG 3 cut(s) 126, 251, 436
BstF5I GGATG 5 cut(s) 84, 94, 305, 579, 672
BstFNI CGCG 2 cut(s) 490, 504
BstHHI GCGC 1 cut(s) 490
BstKTI GATC 1 cut(s) 81
BstMAI GTCTC 1 cut(s) 135
BstMBI GATC 1 cut(s) 78
BstMWI GCNNNNNNNGC 3 cut(s) 34, 43, 239
BstNSI RCATGY 1 cut(s) 428
BstSLI GKGCMC 1 cut(s) 450
BstUI CGCG 2 cut(s) 490, 504
BstV1I GCAGC 1 cut(s) 60
BsuRI GGCC 2 cut(s) 117, 462
BtsCI GGATG 5 cut(s) 84, 94, 305, 579, 672
BtsIMutI CAGTG 1 cut(s) 441
BveI ACCTGC 1 cut(s) 4
Cac8I GCNNGC 1 cut(s) 534
CfoI GCGC 1 cut(s) 490
Cfr13I GGNCC 2 cut(s) 519, 575
CseI GACGC 1 cut(s) 207
Csp6I GTAC 1 cut(s) 355
CviAII CATG 4 cut(s) 157, 425, 453, 586
CviJI RGCY 8 cut(s) 73, 117, 188, 242, 432, 440, 462, 510
CviKI_1 RGCY 8 cut(s) 73, 117, 188, 242, 432, 440, 462, 510
CviQI GTAC 1 cut(s) 355
DdeI CTNAG 3 cut(s) 126, 251, 436
DpnI GATC 1 cut(s) 80
DpnII GATC 1 cut(s) 78
Eam1104I CTCTTC 2 cut(s) 349, 563
EarI CTCTTC 2 cut(s) 349, 563
Eco47I GGWCC 2 cut(s) 519, 575
Eco57I CTGAAG 1 cut(s) 330
FaeI CATG 4 cut(s) 160, 428, 456, 589
FaqI GGGAC 2 cut(s) 505, 561
FatI CATG 4 cut(s) 156, 424, 452, 585
FbaI TGATCA 1 cut(s) 78
FblI GTMKAC 1 cut(s) 221
Fnu4HI GCNGC 2 cut(s) 35, 74
FokI GGATG 5 cut(s) 71, 81, 292, 566, 659
Fsp4HI GCNGC 2 cut(s) 35, 74
FspBI CTAG 2 cut(s) 29, 651
GlaI GCGC 1 cut(s) 489
GluI GCNGC 2 cut(s) 35, 74
GsaI CCCAGC 2 cut(s) 41, 56
HaeIII GGCC 2 cut(s) 117, 462
HapII CCGG 1 cut(s) 294
HgaI GACGC 1 cut(s) 207
HhaI GCGC 1 cut(s) 490
Hin1I GRCGYC 1 cut(s) 218
Hin1II CATG 4 cut(s) 160, 428, 456, 589
Hin6I GCGC 1 cut(s) 488
HinP1I GCGC 1 cut(s) 488
HinfI GANTC 4 cut(s) 290, 331, 499, 695
HpaII CCGG 1 cut(s) 294
HphI GGTGA 1 cut(s) 73
Hpy166II GTNNAC 2 cut(s) 19, 222
Hpy188I TCNGA 4 cut(s) 163, 289, 527, 677
Hpy188III TCNNGA 3 cut(s) 309, 394, 565
Hpy8I GTNNAC 2 cut(s) 19, 222
Hpy99I CGWCG 2 cut(s) 113, 116
HpyAV CCTTC 1 cut(s) 460
HpyCH4III ACNGT 2 cut(s) 109, 445
HpyCH4IV ACGT 1 cut(s) 66
HpyCH4V TGCA 4 cut(s) 59, 403, 422, 603
HpyF10VI GCNNNNNNNGC 3 cut(s) 34, 43, 239
HpyF3I CTNAG 3 cut(s) 126, 251, 436
HpySE526I ACGT 1 cut(s) 66
Hsp92I GRCGYC 1 cut(s) 218
Hsp92II CATG 4 cut(s) 160, 428, 456, 589
HspAI GCGC 1 cut(s) 488
Ksp22I TGATCA 1 cut(s) 78
Kzo9I GATC 1 cut(s) 78
LmnI GCTCC 1 cut(s) 10
LpnPI CCDG 8 cut(s) 23, 32, 38, 307, 322, 476, 550, 655
Lsp1109I GCAGC 1 cut(s) 60
LweI GCATC 1 cut(s) 314
MaeI CTAG 2 cut(s) 29, 651
MaeII ACGT 1 cut(s) 66
MalI GATC 1 cut(s) 80
MboI GATC 1 cut(s) 78
MboII GAAGA 5 cut(s) 184, 336, 550, 650, 690
MhlI GDGCHC 1 cut(s) 450
MluCI AATT 2 cut(s) 315, 667
MlyI GAGTC 2 cut(s) 284, 340
MmeI TCCRAC 1 cut(s) 682
MnlI CCTC 5 cut(s) 128, 533, 554, 564, 572
MseI TTAA 1 cut(s) 314
MslI CAYNNNNRTG 2 cut(s) 584, 590
MspA1I CMGCKG 1 cut(s) 37
MspI CCGG 1 cut(s) 294
Mva1269I GAATGC 1 cut(s) 536
MvnI CGCG 2 cut(s) 490, 504
MwoI GCNNNNNNNGC 3 cut(s) 34, 43, 239
NdeII GATC 1 cut(s) 78
NlaIII CATG 4 cut(s) 160, 428, 456, 589
NlaIV GGNNCC 2 cut(s) 521, 577
NspI RCATGY 1 cut(s) 428
NspV TTCGAA 1 cut(s) 103
PaqCI CACCTGC 1 cut(s) 4
PciI ACATGT 1 cut(s) 424
PctI GAATGC 1 cut(s) 536
PfeI GAWTC 2 cut(s) 499, 695
PkrI GCNGC 2 cut(s) 36, 75
PleI GAGTC 2 cut(s) 284, 339
PpsI GAGTC 2 cut(s) 284, 339
Ppu21I YACGTR 1 cut(s) 67
PscI ACATGT 1 cut(s) 424
PshBI ATTAAT 1 cut(s) 314
PspFI CCCAGC 2 cut(s) 37, 52
PspN4I GGNNCC 2 cut(s) 521, 577
PspPI GGNCC 2 cut(s) 519, 575
RsaI GTAC 1 cut(s) 356
RsaNI GTAC 1 cut(s) 355
RseI CAYNNNNRTG 2 cut(s) 584, 590
SaqAI TTAA 1 cut(s) 314
SatI GCNGC 2 cut(s) 35, 74
Sau3AI GATC 1 cut(s) 78
Sau96I GGNCC 2 cut(s) 519, 575
ScaI AGTACT 1 cut(s) 356
SchI GAGTC 2 cut(s) 284, 340
SduI GDGCHC 1 cut(s) 450
SetI ASST 6 cut(s) 18, 69, 434, 477, 512, 546
SfaNI GCATC 1 cut(s) 314
SfuI TTCGAA 1 cut(s) 103
SinI GGWCC 2 cut(s) 519, 575
SmiMI CAYNNNNRTG 2 cut(s) 584, 590
SmlI CTYRAG 1 cut(s) 476
SmoI CTYRAG 1 cut(s) 476
Sse9I AATT 2 cut(s) 315, 667
SsiI CCGC 5 cut(s) 8, 35, 91, 335, 504
SspMI CTAG 2 cut(s) 29, 651
TaaI ACNGT 2 cut(s) 109, 445
TaiI ACGT 1 cut(s) 69
TaqI TCGA 2 cut(s) 103, 693
TasI AATT 2 cut(s) 315, 667
TatI WGTACW 1 cut(s) 354
TauI GCSGC 1 cut(s) 37
TfiI GAWTC 2 cut(s) 499, 695
Tru1I TTAA 1 cut(s) 314
Tru9I TTAA 1 cut(s) 314
TscAI CASTG 1 cut(s) 448
TseI GCWGC 1 cut(s) 73
TspRI CASTG 1 cut(s) 448
VpaK11BI GGWCC 2 cut(s) 519, 575
VspI ATTAAT 1 cut(s) 314
XceI RCATGY 1 cut(s) 428
XmiI GTMKAC 1 cut(s) 221
XspI CTAG 2 cut(s) 29, 651
ZrmI AGTACT 1 cut(s) 356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.