Rh7DG022600

Protein of unknown function, DUF599

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0015034
Physical Location & Seq
Forward (+)
1777237 .. 1779450
2214 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG022600.1

Sequence Viewer

Length: 723 bp
ATGGAAGAGGAGGCTTTGGACTATGTTCTGGTGCCACTGGGTCTGTTTGTTCTGGGAACGTATCATGTTTGGCTTCTCATCACTGTGCTACGTCATCCAATACGAACTGTGATCGGTCTCAATGCTCAGTCTCGTCACCAATGGGTCTTATCCATGATGAGTGACCCTTTGAAGAATGGTGTTTTGGCTGTTCAGACAATACGCAACAACATAATGGCATCCACACTTTTGGCAACAACAGCTATCACTCTCAGTTCTCTTATCAGTGTATTTGTGAGCAGTACATCAAACTCTGGCAACACCTCGAAACTAATTTATGGCAATAAGACAGCCACTCTCTCTTCAATAAAGTACTTCTCTATCTTGCTGTGCTTTCTTGTTGCCTTTCTCTGCAATGTGCAGTCCATTAGATATTATGCCCATGTTAGCTTCTTGGCCACTGTGCCAACATGGCAAGGTAGAAAGGATTGTATTGAGTACGTTGCAAGAAACTTGAATCGGGGAAGCTACTTTTGGTCACTTGGATTGCGAGCTTTCTACTTCTCATTCCCTCTCTTCCTTTGGATATTTGGGCCTATACCCATGTTTGCATGTAGCTGCGTTATCTCTTGTGTTCTTTATTTCCTGGACACCACCACCAGCTTCACACGACATCTTCACAGTCACTCGATCAAAGAGGTGAAAACTGATAATGATCTAGAATCACTCGGACAATTATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF599

Protein Analysis

240

Amino Acids

27.02

Weight (kDa)

8.92

Isoelectric Point (pI)

37.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF599 PF04654 9 - 213 2.5e-73 Protein of unknown function, DUF599
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015034)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G18215 AT3G18215
fragaria_vesca FvH4_5g16810
malus_domestica MD06G1222100.v1.1 MD15G1311900.v1.1
prunus_persica Prupe.5G226500_v2.0.a1 Prupe.5G226500_v2.0.a1
pyrus_communis pycom06g19760
rosa_chinensis RchiOBHm_Chr7g0179261
rosa_laevigata RLG00000005368
rosa_multiflora Rmu_sc0006939.1_g000006
rosa_roxburghii Rroxscaffold_3G00274170
rosa_rugosa Rorug06G0422800
rosa_samantha Rh7CG023300 Rh7DG022600
rosa_wichuraiana Rw7G001820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 31
AcoI YGGCCR 1 cut(s) 435
AfaI GTAC 3 cut(s) 283, 353, 479
AgsI TTSAA 3 cut(s) 172, 345, 496
AjnI CCWGG 1 cut(s) 624
AjuI GAANNNNNNNTTGG 4 cut(s) 167, 199, 496, 528
AluBI AGCT 6 cut(s) 242, 429, 507, 533, 597, 642
AluI AGCT 6 cut(s) 242, 429, 507, 533, 597, 642
Alw26I GTCTC 2 cut(s) 122, 135
AoxI GGCC 2 cut(s) 435, 572
ApeKI GCWGC 1 cut(s) 597
AspS9I GGNCC 1 cut(s) 572
AsuHPI GGTGA 2 cut(s) 128, 691
BalI TGGCCA 1 cut(s) 437
BanI GGYRCC 1 cut(s) 31
BbvI GCAGC 1 cut(s) 584
BciT130I CCWGG 1 cut(s) 626
BcoDI GTCTC 2 cut(s) 122, 135
BfaI CTAG 1 cut(s) 698
BglI GCCNNNNNGGC 1 cut(s) 451
BisI GCNGC 1 cut(s) 598
BlsI GCNGC 1 cut(s) 599
BmcAI AGTACT 1 cut(s) 353
Bme1390I CCNGG 1 cut(s) 626
BmgT120I GGNCC 1 cut(s) 572
BmiI GGNNCC 1 cut(s) 33
BmrFI CCNGG 1 cut(s) 626
BmrI ACTGGG 1 cut(s) 47
BmsI GCATC 1 cut(s) 227
BmuI ACTGGG 1 cut(s) 47
BsaBI GATNNNNATC 1 cut(s) 693
BsaI GGTCTC 1 cut(s) 122
Bse1I ACTGG 1 cut(s) 42
Bse3DI GCAATG 1 cut(s) 400
Bse8I GATNNNNATC 1 cut(s) 693
BseBI CCWGG 1 cut(s) 626
BseGI GGATG 2 cut(s) 94, 218
BseJI GATNNNNATC 1 cut(s) 693
BseMI GCAATG 1 cut(s) 400
BseMII CTCAG 2 cut(s) 140, 265
BseNI ACTGG 1 cut(s) 42
BseRI GAGGAG 1 cut(s) 23
BseXI GCAGC 1 cut(s) 584
BsgI GTGCAG 1 cut(s) 419
BshFI GGCC 2 cut(s) 437, 574
BshNI GGYRCC 1 cut(s) 31
BsmAI GTCTC 2 cut(s) 122, 135
BsnI GGCC 2 cut(s) 437, 574
Bso31I GGTCTC 1 cut(s) 122
Bsp143I GATC 3 cut(s) 111, 669, 694
BspANI GGCC 2 cut(s) 437, 574
BspCNI CTCAG 2 cut(s) 139, 264
BspLI GGNNCC 1 cut(s) 33
BspT107I GGYRCC 1 cut(s) 31
BspTNI GGTCTC 1 cut(s) 122
BsrDI GCAATG 1 cut(s) 400
BsrI ACTGG 1 cut(s) 42
BssMI GATC 3 cut(s) 111, 669, 694
Bst2UI CCWGG 1 cut(s) 626
Bst4CI ACNGT 4 cut(s) 85, 109, 442, 662
Bst6I CTCTTC 2 cut(s) 346, 560
BstC8I GCNNGC 1 cut(s) 531
BstDEI CTNAG 2 cut(s) 126, 251
BstF5I GGATG 2 cut(s) 94, 218
BstKTI GATC 3 cut(s) 114, 672, 697
BstMAI GTCTC 2 cut(s) 122, 135
BstMBI GATC 3 cut(s) 111, 669, 694
BstMWI GCNNNNNNNGC 2 cut(s) 239, 451
BstNI CCWGG 1 cut(s) 626
BstNSI RCATGY 1 cut(s) 594
BstSCI CCNGG 1 cut(s) 624
BstV1I GCAGC 1 cut(s) 584
BstXI CCANNNNNNTGG 1 cut(s) 229
BsuRI GGCC 2 cut(s) 437, 574
BtsCI GGATG 2 cut(s) 94, 218
BtsIMutI CAGTG 4 cut(s) 35, 81, 271, 438
Cac8I GCNNGC 1 cut(s) 531
Cfr13I GGNCC 1 cut(s) 572
Csp6I GTAC 3 cut(s) 282, 352, 478
CviAII CATG 6 cut(s) 65, 154, 422, 450, 583, 591
CviQI GTAC 3 cut(s) 282, 352, 478
DdeI CTNAG 2 cut(s) 126, 251
DpnI GATC 3 cut(s) 113, 671, 696
DpnII GATC 3 cut(s) 111, 669, 694
EaeI YGGCCR 1 cut(s) 435
Eam1104I CTCTTC 2 cut(s) 346, 560
EarI CTCTTC 2 cut(s) 346, 560
Eco31I GGTCTC 1 cut(s) 122
EcoRII CCWGG 1 cut(s) 624
FaeI CATG 6 cut(s) 68, 157, 425, 453, 586, 594
FatI CATG 6 cut(s) 64, 153, 421, 449, 582, 590
Fnu4HI GCNGC 1 cut(s) 598
FokI GGATG 2 cut(s) 81, 205
Fsp4HI GCNGC 1 cut(s) 598
FspBI CTAG 1 cut(s) 698
GluI GCNGC 1 cut(s) 598
HaeIII GGCC 2 cut(s) 437, 574
Hin1II CATG 6 cut(s) 68, 157, 425, 453, 586, 594
HinfI GANTC 2 cut(s) 496, 701
HphI GGTGA 2 cut(s) 128, 691
Hpy188I TCNGA 2 cut(s) 195, 710
Hpy188III TCNNGA 1 cut(s) 698
HpyCH4III ACNGT 4 cut(s) 85, 109, 442, 662
HpyCH4IV ACGT 3 cut(s) 59, 91, 480
HpyCH4V TGCA 4 cut(s) 393, 400, 485, 590
HpyF10VI GCNNNNNNNGC 2 cut(s) 239, 451
HpyF3I CTNAG 2 cut(s) 126, 251
HpySE526I ACGT 3 cut(s) 59, 91, 480
Hsp92II CATG 6 cut(s) 68, 157, 425, 453, 586, 594
Kzo9I GATC 3 cut(s) 111, 669, 694
LpnPI CCDG 7 cut(s) 14, 23, 38, 279, 611, 638, 652
Lsp1109I GCAGC 1 cut(s) 584
LweI GCATC 1 cut(s) 227
MaeI CTAG 1 cut(s) 698
MaeII ACGT 3 cut(s) 59, 91, 480
MaeIII GTNAC 4 cut(s) 134, 161, 516, 662
MalI GATC 3 cut(s) 113, 671, 696
MboI GATC 3 cut(s) 111, 669, 694
MboII GAAGA 5 cut(s) 17, 184, 333, 547, 647
MlsI TGGCCA 1 cut(s) 437
MluCI AATT 2 cut(s) 312, 713
MluNI TGGCCA 1 cut(s) 437
MnlI CCTC 4 cut(s) 4, 313, 561, 670
Mox20I TGGCCA 1 cut(s) 437
MscI TGGCCA 1 cut(s) 437
MslI CAYNNNNRTG 1 cut(s) 83
Msp20I TGGCCA 1 cut(s) 437
MspR9I CCNGG 1 cut(s) 626
MvaI CCWGG 1 cut(s) 626
MwoI GCNNNNNNNGC 2 cut(s) 239, 451
NdeII GATC 3 cut(s) 111, 669, 694
NlaIII CATG 6 cut(s) 68, 157, 425, 453, 586, 594
NlaIV GGNNCC 1 cut(s) 33
NmuCI GTSAC 4 cut(s) 134, 161, 516, 662
NspI RCATGY 1 cut(s) 594
PfeI GAWTC 2 cut(s) 496, 701
PfoI TCCNGGA 1 cut(s) 624
PkrI GCNGC 1 cut(s) 599
Psp6I CCWGG 1 cut(s) 624
PspGI CCWGG 1 cut(s) 624
PspN4I GGNNCC 1 cut(s) 33
PspPI GGNCC 1 cut(s) 572
RsaI GTAC 3 cut(s) 283, 353, 479
RsaNI GTAC 3 cut(s) 282, 352, 478
RseI CAYNNNNRTG 1 cut(s) 83
SatI GCNGC 1 cut(s) 598
Sau3AI GATC 3 cut(s) 111, 669, 694
Sau96I GGNCC 1 cut(s) 572
ScaI AGTACT 1 cut(s) 353
ScrFI CCNGG 1 cut(s) 626
SfaNI GCATC 1 cut(s) 227
SmiMI CAYNNNNRTG 1 cut(s) 83
Sse9I AATT 2 cut(s) 312, 713
SspMI CTAG 1 cut(s) 698
StyD4I CCNGG 1 cut(s) 624
TaaI ACNGT 4 cut(s) 85, 109, 442, 662
TaiI ACGT 3 cut(s) 62, 94, 483
TaqI TCGA 2 cut(s) 305, 668
TaqII GACCGA 1 cut(s) 104
TasI AATT 2 cut(s) 312, 713
TatI WGTACW 2 cut(s) 281, 351
TfiI GAWTC 2 cut(s) 496, 701
TscAI CASTG 4 cut(s) 42, 88, 271, 445
TseFI GTSAC 4 cut(s) 134, 161, 516, 662
TseI GCWGC 1 cut(s) 597
Tsp45I GTSAC 4 cut(s) 134, 161, 516, 662
TspRI CASTG 4 cut(s) 42, 88, 271, 445
XbaI TCTAGA 1 cut(s) 697
XceI RCATGY 1 cut(s) 594
XspI CTAG 1 cut(s) 698
ZrmI AGTACT 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.