MD06G1226100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0011239
Physical Location & Seq
Forward (+)
35657326 .. 35660583
3258 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1226100.v1.1.491

Sequence Viewer

Length: 432 bp
ATGGCGAAACCGGTTGCAACCGCCGCGTCATCCCTCTCCCAAACCCTAAAGCGCTACCTCAAGAAACCGTGGGAGATAACCGGTCCGTGCGCGGATCCGGAGTACAAATTGGCCGTACCCGGCGCGCTCGAGTACCGGCTCGAGTGCCCTGCCACCACCAAGGTCCAGGCGTGCGTGCCCACCTCCAACCCGGAAACCGTTTACGATATCAAGTATTTCGCCCGCGACCAGCGGCGCAACCGGCCCCCGATCAAGCGCACCGTATTGAAGAAGGCCGATGTGGAGAAGCTGATGAAGGAGAAGACGACCTTCGACGTCTCGGAATTTCCGCCGGTTTACTTGACCGATTTCGTTGAAGAGGATTACAATGCTCAGGGCTTACGGGATTCTGGAAAAAATATCTGTGTTATTTTATTCCTCTCCTTGAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

144

Amino Acids

16.19

Weight (kDa)

8.84

Isoelectric Point (pI)

47.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 318
AccII CGCG 4 cut(s) 26, 92, 125, 225
AccIII TCCGGA 1 cut(s) 97
AciI CCGC 6 cut(s) 21, 24, 92, 223, 232, 329
AclWI GGATC 2 cut(s) 89, 102
AcoI YGGCCR 1 cut(s) 111
AcsI RAATTY 1 cut(s) 323
AcyI GRCGYC 1 cut(s) 315
AfaI GTAC 3 cut(s) 104, 117, 134
AfeI AGCGCT 1 cut(s) 53
AgeI ACCGGT 2 cut(s) 10, 80
AgsI TTSAA 3 cut(s) 268, 356, 427
AjnI CCWGG 1 cut(s) 165
AluBI AGCT 1 cut(s) 289
AluI AGCT 1 cut(s) 289
Alw26I GTCTC 1 cut(s) 322
AlwI GGATC 2 cut(s) 89, 102
Ama87I CYCGRG 2 cut(s) 128, 140
Aor13HI TCCGGA 1 cut(s) 97
Aor51HI AGCGCT 1 cut(s) 53
AoxI GGCC 3 cut(s) 111, 242, 273
ApoI RAATTY 1 cut(s) 323
AsiGI ACCGGT 2 cut(s) 10, 80
AspLEI GCGC 6 cut(s) 54, 92, 125, 127, 237, 258
AspS9I GGNCC 3 cut(s) 83, 163, 243
AsuC2I CCSGG 2 cut(s) 120, 191
AvaI CYCGRG 2 cut(s) 128, 140
AvaII GGWCC 2 cut(s) 83, 163
BaeGI GKGCMC 2 cut(s) 149, 180
BamHI GGATCC 1 cut(s) 94
BbsI GAAGAC 1 cut(s) 308
BceAI ACGGC 1 cut(s) 98
BcgI CGANNNNNNTGC 2 cut(s) 131, 165
BciT130I CCWGG 1 cut(s) 167
BcnI CCSGG 2 cut(s) 120, 191
BcoDI GTCTC 1 cut(s) 322
BfoI RGCGCY 1 cut(s) 55
BisI GCNGC 2 cut(s) 24, 233
BlsI GCNGC 2 cut(s) 25, 234
Bme1390I CCNGG 3 cut(s) 120, 167, 191
Bme18I GGWCC 2 cut(s) 83, 163
BmeT110I CYCGRG 2 cut(s) 128, 140
BmgT120I GGNCC 3 cut(s) 83, 163, 243
BmiI GGNNCC 2 cut(s) 96, 245
BmrFI CCNGG 3 cut(s) 120, 167, 191
BpiI GAAGAC 1 cut(s) 308
Bpu10I CCTNAGC 1 cut(s) 372
BpuEI CTTGAG 1 cut(s) 44
BpuMI CCSGG 2 cut(s) 120, 191
BsaHI GRCGYC 1 cut(s) 315
BsaJI CCNNGG 2 cut(s) 68, 159
BsaWI WCCGGW 3 cut(s) 10, 80, 97
Bse118I RCCGGY 5 cut(s) 10, 80, 135, 240, 331
BseAI TCCGGA 1 cut(s) 97
BseBI CCWGG 1 cut(s) 167
BseDI CCNNGG 2 cut(s) 68, 159
BseGI GGATG 1 cut(s) 29
BseMII CTCAG 1 cut(s) 386
BsePI GCGCGC 1 cut(s) 123
BseSI GKGCMC 2 cut(s) 149, 180
Bsh1236I CGCG 4 cut(s) 26, 92, 125, 225
BshFI GGCC 3 cut(s) 113, 244, 275
BshTI ACCGGT 2 cut(s) 10, 80
BsiHKCI CYCGRG 2 cut(s) 128, 140
BsiSI CCGG 8 cut(s) 11, 81, 98, 120, 136, 191, 241, 332
BsmAI GTCTC 1 cut(s) 322
BsmBI CGTCTC 1 cut(s) 322
BsnI GGCC 3 cut(s) 113, 244, 275
BsoBI CYCGRG 2 cut(s) 128, 140
Bsp1286I GDGCHC 2 cut(s) 149, 180
Bsp13I TCCGGA 1 cut(s) 97
Bsp143I GATC 2 cut(s) 94, 249
BspACI CCGC 6 cut(s) 21, 24, 92, 223, 232, 329
BspANI GGCC 3 cut(s) 113, 244, 275
BspCNI CTCAG 1 cut(s) 385
BspEI TCCGGA 1 cut(s) 97
BspFNI CGCG 4 cut(s) 26, 92, 125, 225
BspLI GGNNCC 2 cut(s) 96, 245
BspPI GGATC 2 cut(s) 89, 102
BsrFI RCCGGY 5 cut(s) 10, 80, 135, 240, 331
BssAI RCCGGY 5 cut(s) 10, 80, 135, 240, 331
BssECI CCNNGG 2 cut(s) 68, 159
BssHII GCGCGC 1 cut(s) 123
BssMI GATC 2 cut(s) 94, 249
BssNI GRCGYC 1 cut(s) 315
BssT1I CCWWGG 1 cut(s) 159
Bst2UI CCWGG 1 cut(s) 167
Bst4CI ACNGT 3 cut(s) 69, 199, 262
Bst6I CTCTTC 1 cut(s) 351
BstACI GRCGYC 1 cut(s) 315
BstC8I GCNNGC 4 cut(s) 125, 172, 176, 223
BstDEI CTNAG 1 cut(s) 372
BstDSI CCRYGG 1 cut(s) 68
BstF5I GGATG 1 cut(s) 29
BstFNI CGCG 4 cut(s) 26, 92, 125, 225
BstH2I RGCGCY 1 cut(s) 55
BstHHI GCGC 6 cut(s) 54, 92, 125, 127, 237, 258
BstKTI GATC 2 cut(s) 97, 252
BstMAI GTCTC 1 cut(s) 322
BstMBI GATC 2 cut(s) 94, 249
BstMWI GCNNNNNNNGC 2 cut(s) 23, 241
BstNI CCWGG 1 cut(s) 167
BstSCI CCNGG 3 cut(s) 118, 165, 189
BstSLI GKGCMC 2 cut(s) 149, 180
BstUI CGCG 4 cut(s) 26, 92, 125, 225
BstV2I GAAGAC 1 cut(s) 308
BstX2I RGATCY 1 cut(s) 94
BstYI RGATCY 1 cut(s) 94
BsuRI GGCC 3 cut(s) 113, 244, 275
BtgI CCRYGG 1 cut(s) 68
BtsCI GGATG 1 cut(s) 29
Cac8I GCNNGC 4 cut(s) 125, 172, 176, 223
CfoI GCGC 6 cut(s) 54, 92, 125, 127, 237, 258
Cfr10I RCCGGY 5 cut(s) 10, 80, 135, 240, 331
Cfr13I GGNCC 3 cut(s) 83, 163, 243
CpoI CGGWCCG 1 cut(s) 83
CseI GACGC 1 cut(s) 15
Csp6I GTAC 3 cut(s) 103, 116, 133
CspAI ACCGGT 2 cut(s) 10, 80
CspI CGGWCCG 1 cut(s) 83
CviJI RGCY 6 cut(s) 113, 139, 244, 275, 289, 378
CviKI_1 RGCY 6 cut(s) 113, 139, 244, 275, 289, 378
CviQI GTAC 3 cut(s) 103, 116, 133
DdeI CTNAG 1 cut(s) 372
DpnI GATC 2 cut(s) 96, 251
DpnII GATC 2 cut(s) 94, 249
EaeI YGGCCR 1 cut(s) 111
Eam1104I CTCTTC 1 cut(s) 351
EarI CTCTTC 1 cut(s) 351
EciI GGCGGA 1 cut(s) 318
Eco130I CCWWGG 1 cut(s) 159
Eco32I GATATC 1 cut(s) 208
Eco47I GGWCC 2 cut(s) 83, 163
Eco47III AGCGCT 1 cut(s) 53
Eco88I CYCGRG 2 cut(s) 128, 140
EcoRII CCWGG 1 cut(s) 165
EcoRV GATATC 1 cut(s) 208
EcoT14I CCWWGG 1 cut(s) 159
ErhI CCWWGG 1 cut(s) 159
Esp3I CGTCTC 1 cut(s) 322
FalI AAGNNNNNCTT 2 cut(s) 293, 325
FauI CCCGC 1 cut(s) 230
Fnu4HI GCNGC 2 cut(s) 24, 233
FokI GGATG 1 cut(s) 16
Fsp4HI GCNGC 2 cut(s) 24, 233
GlaI GCGC 6 cut(s) 53, 91, 124, 126, 236, 257
GluI GCNGC 2 cut(s) 24, 233
HaeII RGCGCY 1 cut(s) 55
HaeIII GGCC 3 cut(s) 113, 244, 275
HapII CCGG 8 cut(s) 11, 81, 98, 120, 136, 191, 241, 332
HgaI GACGC 1 cut(s) 15
HhaI GCGC 6 cut(s) 54, 92, 125, 127, 237, 258
Hin1I GRCGYC 1 cut(s) 315
Hin6I GCGC 6 cut(s) 52, 90, 123, 125, 235, 256
HinP1I GCGC 6 cut(s) 52, 90, 123, 125, 235, 256
HinfI GANTC 1 cut(s) 386
HpaII CCGG 8 cut(s) 11, 81, 98, 120, 136, 191, 241, 332
Hpy166II GTNNAC 2 cut(s) 202, 337
Hpy188I TCNGA 1 cut(s) 322
Hpy188III TCNNGA 3 cut(s) 61, 98, 390
Hpy8I GTNNAC 2 cut(s) 202, 337
Hpy99I CGWCG 1 cut(s) 317
HpyAV CCTTC 3 cut(s) 265, 289, 319
HpyCH4III ACNGT 3 cut(s) 69, 199, 262
HpyCH4IV ACGT 1 cut(s) 315
HpyCH4V TGCA 1 cut(s) 17
HpyF10VI GCNNNNNNNGC 2 cut(s) 23, 241
HpyF3I CTNAG 1 cut(s) 372
HpySE526I ACGT 1 cut(s) 315
Hsp92I GRCGYC 1 cut(s) 315
HspAI GCGC 6 cut(s) 52, 90, 123, 125, 235, 256
Kpn2I TCCGGA 1 cut(s) 97
Kzo9I GATC 2 cut(s) 94, 249
MaeII ACGT 1 cut(s) 315
MalI GATC 2 cut(s) 96, 251
MboI GATC 2 cut(s) 94, 249
MboII GAAGA 3 cut(s) 280, 313, 368
MflI RGATCY 1 cut(s) 94
MhlI GDGCHC 2 cut(s) 149, 180
MluCI AATT 2 cut(s) 107, 323
MmeI TCCRAC 1 cut(s) 210
MnlI CCTC 5 cut(s) 44, 68, 193, 352, 428
MroI TCCGGA 1 cut(s) 97
MspA1I CMGCKG 1 cut(s) 232
MspI CCGG 8 cut(s) 11, 81, 98, 120, 136, 191, 241, 332
MspR9I CCNGG 3 cut(s) 120, 167, 191
MvaI CCWGG 1 cut(s) 167
MvnI CGCG 4 cut(s) 26, 92, 125, 225
MwoI GCNNNNNNNGC 2 cut(s) 23, 241
NciI CCSGG 2 cut(s) 120, 191
NdeII GATC 2 cut(s) 94, 249
NlaIV GGNNCC 2 cut(s) 96, 245
PaeR7I CTCGAG 2 cut(s) 128, 140
PauI GCGCGC 1 cut(s) 123
PfeI GAWTC 1 cut(s) 386
PinAI ACCGGT 2 cut(s) 10, 80
PkrI GCNGC 2 cut(s) 25, 234
Psp6I CCWGG 1 cut(s) 165
PspGI CCWGG 1 cut(s) 165
PspN4I GGNNCC 2 cut(s) 96, 245
PspPI GGNCC 3 cut(s) 83, 163, 243
PspXI VCTCGAGB 2 cut(s) 128, 140
PsuI RGATCY 1 cut(s) 94
PteI GCGCGC 1 cut(s) 123
RsaI GTAC 3 cut(s) 104, 117, 134
RsaNI GTAC 3 cut(s) 103, 116, 133
Rsr2I CGGWCCG 1 cut(s) 83
RsrII CGGWCCG 1 cut(s) 83
SatI GCNGC 2 cut(s) 24, 233
Sau3AI GATC 2 cut(s) 94, 249
Sau96I GGNCC 3 cut(s) 83, 163, 243
ScrFI CCNGG 3 cut(s) 120, 167, 191
SduI GDGCHC 2 cut(s) 149, 180
SetI ASST 6 cut(s) 60, 165, 185, 291, 311, 318
Sfr274I CTCGAG 2 cut(s) 128, 140
SinI GGWCC 2 cut(s) 83, 163
SlaI CTCGAG 2 cut(s) 128, 140
SmlI CTYRAG 3 cut(s) 59, 128, 140
SmoI CTYRAG 3 cut(s) 59, 128, 140
Sse9I AATT 2 cut(s) 107, 323
SsiI CCGC 6 cut(s) 21, 24, 92, 223, 232, 329
StyD4I CCNGG 3 cut(s) 118, 165, 189
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 3 cut(s) 69, 199, 262
TaiI ACGT 1 cut(s) 318
TaqI TCGA 3 cut(s) 129, 141, 312
TaqII GACCGA 1 cut(s) 359
TasI AATT 2 cut(s) 107, 323
TatI WGTACW 1 cut(s) 102
TauI GCSGC 2 cut(s) 26, 235
TfiI GAWTC 1 cut(s) 386
TspDTI ATGAA 1 cut(s) 308
TspGWI ACGGA 1 cut(s) 75
VpaK11BI GGWCC 2 cut(s) 83, 163
XapI RAATTY 1 cut(s) 323
XhoI CTCGAG 2 cut(s) 128, 140
ZraI GACGTC 1 cut(s) 316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.