MD14G1213000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011239
Physical Location & Seq
Forward (+)
29757441 .. 29759553
2113 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1213000.v1.1.491

Sequence Viewer

Length: 456 bp
ATGGCGAAATCGGTCTCCACCGCCGTGTCATCCCTCGCTCAAACCCTAAAGCGGTACTTCAAGAAACCGTGGGAAATAACCGGCCCGTGTGCCGACCCGGAGTACAAATTAGCAGTGCCCGGCGCGCTCGAGTACCGGGTCGAGTGCCCAGCCACGACCAAGGTCCAGGCCTGCGTGCCCACCTCCAACCCGGAAACCGTGTACGACATCAAGTACTATACCCGCGACCAGAGGCGCAACCGGCCACCGATTAGACGCACCGTTTTGAAGAAGGCGGATGTGGAGAAGATGATGAAGGAGAGGACGACATTCAACGTCTCGGAATTCCCACCTGTTTACTTGGCCGAAGCCGTTGAAGAGGATTACAATGCTCGAGGCTTATGGGATTCTGGAAAAATATGTGTGTTATTTTGTTCCTCTCGTCGAACTAAAGAGATGCTGTTCATGTTAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

152

Amino Acids

17.31

Weight (kDa)

9.21

Isoelectric Point (pI)

49.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 125, 225
AciI CCGC 4 cut(s) 21, 52, 223, 275
AcoI YGGCCR 2 cut(s) 242, 342
AcsI RAATTY 1 cut(s) 323
AfaI GTAC 5 cut(s) 56, 104, 134, 203, 215
AfiI CCNNNNNNNGG 1 cut(s) 51
AgsI TTSAA 4 cut(s) 61, 268, 313, 356
AjnI CCWGG 1 cut(s) 165
AleI CACNNNNGTG 1 cut(s) 23
Alw26I GTCTC 2 cut(s) 19, 322
Ama87I CYCGRG 2 cut(s) 128, 372
AoxI GGCC 4 cut(s) 82, 168, 242, 342
ApoI RAATTY 1 cut(s) 323
AspLEI GCGC 3 cut(s) 125, 127, 237
AspS9I GGNCC 2 cut(s) 83, 163
AsuC2I CCSGG 4 cut(s) 98, 120, 137, 191
AvaI CYCGRG 2 cut(s) 128, 372
AvaII GGWCC 1 cut(s) 163
BaeGI GKGCMC 3 cut(s) 120, 149, 180
BceAI ACGGC 2 cut(s) 8, 335
BciT130I CCWGG 1 cut(s) 167
BcnI CCSGG 4 cut(s) 98, 120, 137, 191
BcoDI GTCTC 2 cut(s) 19, 322
BmcAI AGTACT 1 cut(s) 215
Bme1390I CCNGG 5 cut(s) 98, 120, 137, 167, 191
Bme18I GGWCC 1 cut(s) 163
BmeT110I CYCGRG 2 cut(s) 128, 372
BmgT120I GGNCC 2 cut(s) 83, 163
BmrFI CCNGG 5 cut(s) 98, 120, 137, 167, 191
BmsI GCATC 1 cut(s) 426
BoxI GACNNNNGTC 1 cut(s) 161
BpuMI CCSGG 4 cut(s) 98, 120, 137, 191
BsaI GGTCTC 1 cut(s) 19
BsaJI CCNNGG 2 cut(s) 68, 159
Bsc4I CCNNNNNNNGG 1 cut(s) 51
Bse118I RCCGGY 2 cut(s) 80, 240
BseBI CCWGG 1 cut(s) 167
BseDI CCNNGG 2 cut(s) 68, 159
BseGI GGATG 2 cut(s) 29, 283
BseLI CCNNNNNNNGG 1 cut(s) 51
BsePI GCGCGC 1 cut(s) 123
BseSI GKGCMC 3 cut(s) 120, 149, 180
BseYI CCCAGC 1 cut(s) 148
Bsh1236I CGCG 2 cut(s) 125, 225
BshFI GGCC 4 cut(s) 84, 170, 244, 344
BsiHKCI CYCGRG 2 cut(s) 128, 372
BsiSI CCGG 6 cut(s) 81, 98, 120, 136, 191, 241
BslI CCNNNNNNNGG 1 cut(s) 51
BsmAI GTCTC 2 cut(s) 19, 322
BsmBI CGTCTC 1 cut(s) 322
BsnI GGCC 4 cut(s) 84, 170, 244, 344
Bso31I GGTCTC 1 cut(s) 19
BsoBI CYCGRG 2 cut(s) 128, 372
Bsp1286I GDGCHC 3 cut(s) 120, 149, 180
BspACI CCGC 4 cut(s) 21, 52, 223, 275
BspANI GGCC 4 cut(s) 84, 170, 244, 344
BspFNI CGCG 2 cut(s) 125, 225
BspTNI GGTCTC 1 cut(s) 19
BsrFI RCCGGY 2 cut(s) 80, 240
BssAI RCCGGY 2 cut(s) 80, 240
BssECI CCNNGG 2 cut(s) 68, 159
BssHII GCGCGC 1 cut(s) 123
BssT1I CCWWGG 1 cut(s) 159
Bst2UI CCWGG 1 cut(s) 167
Bst4CI ACNGT 3 cut(s) 69, 199, 262
Bst6I CTCTTC 1 cut(s) 351
BstC8I GCNNGC 3 cut(s) 125, 172, 176
BstDSI CCRYGG 1 cut(s) 68
BstF5I GGATG 2 cut(s) 29, 283
BstFNI CGCG 2 cut(s) 125, 225
BstHHI GCGC 3 cut(s) 125, 127, 237
BstMAI GTCTC 2 cut(s) 19, 322
BstMWI GCNNNNNNNGC 2 cut(s) 124, 241
BstNI CCWGG 1 cut(s) 167
BstPAI GACNNNNGTC 1 cut(s) 161
BstSCI CCNGG 5 cut(s) 96, 118, 135, 165, 189
BstSLI GKGCMC 3 cut(s) 120, 149, 180
BstUI CGCG 2 cut(s) 125, 225
BsuRI GGCC 4 cut(s) 84, 170, 244, 344
BtgI CCRYGG 1 cut(s) 68
BtsCI GGATG 2 cut(s) 29, 283
BtsI GCAGTG 1 cut(s) 120
BtsIMutI CAGTG 1 cut(s) 120
Cac8I GCNNGC 3 cut(s) 125, 172, 176
CfoI GCGC 3 cut(s) 125, 127, 237
Cfr10I RCCGGY 2 cut(s) 80, 240
Cfr13I GGNCC 2 cut(s) 83, 163
CseI GACGC 1 cut(s) 264
Csp6I GTAC 5 cut(s) 55, 103, 133, 202, 214
CviAII CATG 2 cut(s) 445, 453
CviJI RGCY 7 cut(s) 84, 152, 170, 244, 344, 350, 378
CviKI_1 RGCY 7 cut(s) 84, 152, 170, 244, 344, 350, 378
CviQI GTAC 5 cut(s) 55, 103, 133, 202, 214
EaeI YGGCCR 2 cut(s) 242, 342
Eam1104I CTCTTC 1 cut(s) 351
EarI CTCTTC 1 cut(s) 351
EciI GGCGGA 1 cut(s) 290
Eco130I CCWWGG 1 cut(s) 159
Eco147I AGGCCT 1 cut(s) 170
Eco31I GGTCTC 1 cut(s) 19
Eco47I GGWCC 1 cut(s) 163
Eco88I CYCGRG 2 cut(s) 128, 372
EcoRI GAATTC 1 cut(s) 323
EcoRII CCWGG 1 cut(s) 165
EcoT14I CCWWGG 1 cut(s) 159
ErhI CCWWGG 1 cut(s) 159
Esp3I CGTCTC 1 cut(s) 322
FaeI CATG 2 cut(s) 448, 456
FaiI YATR 5 cut(s) 219, 382, 400, 446, 454
FalI AAGNNNNNCTT 2 cut(s) 41, 73
FatI CATG 2 cut(s) 444, 452
FauI CCCGC 1 cut(s) 230
FokI GGATG 2 cut(s) 16, 290
GlaI GCGC 3 cut(s) 124, 126, 236
GsaI CCCAGC 1 cut(s) 152
HaeIII GGCC 4 cut(s) 84, 170, 244, 344
HapII CCGG 6 cut(s) 81, 98, 120, 136, 191, 241
HgaI GACGC 1 cut(s) 264
HhaI GCGC 3 cut(s) 125, 127, 237
Hin1II CATG 2 cut(s) 448, 456
Hin6I GCGC 3 cut(s) 123, 125, 235
HinP1I GCGC 3 cut(s) 123, 125, 235
HinfI GANTC 1 cut(s) 386
HpaII CCGG 6 cut(s) 81, 98, 120, 136, 191, 241
Hpy166II GTNNAC 2 cut(s) 202, 337
Hpy188I TCNGA 1 cut(s) 322
Hpy188III TCNNGA 2 cut(s) 61, 390
Hpy8I GTNNAC 2 cut(s) 202, 337
Hpy99I CGWCG 1 cut(s) 426
HpyAV CCTTC 2 cut(s) 265, 289
HpyCH4III ACNGT 3 cut(s) 69, 199, 262
HpyCH4IV ACGT 1 cut(s) 315
HpyF10VI GCNNNNNNNGC 2 cut(s) 124, 241
HpySE526I ACGT 1 cut(s) 315
Hsp92II CATG 2 cut(s) 448, 456
HspAI GCGC 3 cut(s) 123, 125, 235
LweI GCATC 1 cut(s) 426
MaeII ACGT 1 cut(s) 315
MboII GAAGA 3 cut(s) 280, 298, 368
MhlI GDGCHC 3 cut(s) 120, 149, 180
MluCI AATT 2 cut(s) 107, 323
MmeI TCCRAC 1 cut(s) 210
MnlI CCTC 7 cut(s) 44, 193, 225, 294, 352, 368, 427
MslI CAYNNNNRTG 1 cut(s) 23
MspI CCGG 6 cut(s) 81, 98, 120, 136, 191, 241
MspR9I CCNGG 5 cut(s) 98, 120, 137, 167, 191
MvaI CCWGG 1 cut(s) 167
MvnI CGCG 2 cut(s) 125, 225
MwoI GCNNNNNNNGC 2 cut(s) 124, 241
NciI CCSGG 4 cut(s) 98, 120, 137, 191
NlaIII CATG 2 cut(s) 448, 456
OliI CACNNNNGTG 1 cut(s) 23
PaeR7I CTCGAG 2 cut(s) 128, 372
PauI GCGCGC 1 cut(s) 123
PceI AGGCCT 1 cut(s) 170
PfeI GAWTC 1 cut(s) 386
PshAI GACNNNNGTC 1 cut(s) 161
Psp6I CCWGG 1 cut(s) 165
PspFI CCCAGC 1 cut(s) 148
PspGI CCWGG 1 cut(s) 165
PspPI GGNCC 2 cut(s) 83, 163
PspXI VCTCGAGB 2 cut(s) 128, 372
PteI GCGCGC 1 cut(s) 123
RsaI GTAC 5 cut(s) 56, 104, 134, 203, 215
RsaNI GTAC 5 cut(s) 55, 103, 133, 202, 214
RseI CAYNNNNRTG 1 cut(s) 23
Sau96I GGNCC 2 cut(s) 83, 163
ScaI AGTACT 1 cut(s) 215
ScrFI CCNGG 5 cut(s) 98, 120, 137, 167, 191
SduI GDGCHC 3 cut(s) 120, 149, 180
SetI ASST 4 cut(s) 165, 185, 318, 334
SfaNI GCATC 1 cut(s) 426
Sfr274I CTCGAG 2 cut(s) 128, 372
SinI GGWCC 1 cut(s) 163
SlaI CTCGAG 2 cut(s) 128, 372
SmiMI CAYNNNNRTG 1 cut(s) 23
SmlI CTYRAG 2 cut(s) 128, 372
SmoI CTYRAG 2 cut(s) 128, 372
Sse9I AATT 2 cut(s) 107, 323
SseBI AGGCCT 1 cut(s) 170
SsiI CCGC 4 cut(s) 21, 52, 223, 275
StuI AGGCCT 1 cut(s) 170
StyD4I CCNGG 5 cut(s) 96, 118, 135, 165, 189
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 3 cut(s) 69, 199, 262
TaiI ACGT 1 cut(s) 318
TaqI TCGA 4 cut(s) 129, 141, 373, 424
TasI AATT 2 cut(s) 107, 323
TatI WGTACW 2 cut(s) 102, 213
TfiI GAWTC 1 cut(s) 386
TscAI CASTG 1 cut(s) 120
TspDTI ATGAA 2 cut(s) 308, 433
TspRI CASTG 1 cut(s) 120
VpaK11BI GGWCC 1 cut(s) 163
XapI RAATTY 1 cut(s) 323
XhoI CTCGAG 2 cut(s) 128, 372
ZrmI AGTACT 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.