Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0013291

Physical Location & Seq

Genomic Coordinates Reverse (-)

35914293 .. 35917889

Gene Structure 3597 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1229500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1833 bp

ATGACCCCCCAGCTCACCGAGGCCTACGCCTGCGTCCCGTCAACCGAGCGCGGCCGTGGGATTCTGATCTCCGGCGACCCCAAATCCGACAAGCTCCTCTACACCAATGGCAGATCCGTAATTATCATGAGCCTCCAGAACCCCCTCGACGTTGCCGTTTACGCCGAGCACGCTTATCCCGTCACTGTTGCTAGGTTCTCCCCCAACGGCGAGTGGATCGCGTCCGCCGACGTCTCCGGAACCGTCAGGATATGGGGTACCCACAACGACTTCGTATTGAAGAACGAGTTCAAGGTCCTCTCGGGTCGGATCGACGACCTCCAGTGGTCCCCTGACGGGTTGAGGATCGTTGCTTCCGGCGAGGGCAAAGGGAAGTCTTTCGTTCGCGCGTTCATGTGGGATTCAGGCTCAACTGTTGGGGATTTTGATGGGCACTCGAAGCGTGTGTTAAGCTGTGCGTTTAAGCCGACGAGACCCTTTCGAATCGTCACTTGTGGGGAGGATTTCTTGGTGAATTTCTACGAAGGACCGCCTTTCAAATTCAAGCTGTCTCTCAGGGATCATTCGAATTTTGTAAATTGTATAAGGTTCTGTCCAGATGGTAGTAAGTTTATCACTGTAAGCTCAGATAAAAGTGGTATGGTTTATGATGGAAAGACTGCGGAGAAGATCGGAGAGCTGTCCGCCGAAGATGGCCACAAAGGCAGCATTTATGCTCTTAGCTGGAGTCCTGATGGTAAACAGGTTCTGACTGTGTCTGCTGACAAATCGGCAAAAGTATGGGAGATATCTGAGGATAATAATGGGAAGCTGAAGAAAACGTTGCCGCCTCCTGGATCGGGTGGAGTGGATGACATGCTAGTTGGGTGTCTGTGGCAGAATGATCATATTGTTACGGTTTCTCTCGGAGGCTTCATTAGTATATTCTCAGCGAGCAACCTTGATAAAGCCCCTCTACTGATATCCGGACACATGAAGAATGTAACTTCATTAGCTGTTCTAAAGATTGACCCAAAACTTATATTGTCTAGCAGCTATGATGGTCTCATTGTTAAATGGATTCAAGGTGCTGGATATGGTGGGAAGTTACAAAGGAAGGAGAATTCTCAGATAAAATGTTTAGCAGCTGTTGACGAAGAAATCGTTTCATGTGGATTTGACAATAAGGTTTGGAGAGTTCCTCTCCGCAGTGATCAATGTGGAGATGCAGAGGCTATTGATGTTGGAAGTCAGCCAAAGGACATAAGCCTTGCCCTTCTATCTCCTGAACTTGCTTTGGTTTCAACTGATTCTGGAGTTGTCATGCTCCATGGCACAAAAGTTTTGTCAACTATTAACCTTGGATTCACTGTGACTGCATGTACTATATCACCGGATGGAAGTGAAGCCATCATTGGTGGGCAGGACGGTAAACTGCATATGTATTCTGTAACAGGTGATACACTTAAGGAAGAGGCTGTTTTGGAGAAGCATCGGGGGCCCATTTCTGTCATACGCTACTCTCCCGATGTTTCTATGTTTGCATCAGGAGATTTGAACCGAGAAGCTGTTGTCTGGGACCGTGCCTCTCGAGAGATCAAGCTTAAGAACATGCTGTACCATACTGCGCGAATAAACTGCCTTGCTTGGTCTCCTGATAGCACGATGGTTGCAACTGGATCTCTTGACACGTGTGTTATCATATACGAAGTCGACAAGCCTCCAGCAAGCCGTACAACCATTAAGGGTGCAAACTTGGGTGGGGTGTACGGCTTAGCTTTCATTGATGAGCACACTGTGGTCAGCTCTGGTGAGGATGCATTTATTCGTGTCTGGAGGTTGACTCCGCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005856 GO:0005884 GO:0005938 GO:0006996 GO:0007010 GO:0007015 GO:0008064 GO:0008092 GO:0008150 GO:0008154 GO:0009987 GO:0010638 GO:0015629 GO:0016043 GO:0022411 GO:0030029 GO:0030036 GO:0030042 GO:0030832 GO:0030834 GO:0030836 GO:0030863 GO:0030864 GO:0031461 GO:0032535 GO:0032956 GO:0032970 GO:0032984 GO:0032991 GO:0033043 GO:0042641 GO:0042643 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043243 GO:0043244 GO:0043624 GO:0043933 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0044877 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051015 GO:0051128 GO:0051130 GO:0051261 GO:0051493 GO:0051495 GO:0065007 GO:0065008 GO:0071840 GO:0071944 GO:0080008 GO:0090066 GO:0097435 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0110053 GO:1901879 GO:1901881 GO:1902494 GO:1902903 GO:1902905 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

611

Amino Acids

66.15

Weight (kDa)

6.0

Isoelectric Point (pI)

28.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_WDHD1_1st	PF24817	34 - 116	4e-06	WDHD1 first WD40 domain
Beta-prop_WDR3_1st	PF25173	39 - 174	1.1e-08	WDR3 first beta-propeller domain
Beta-prop_EML	PF23409	54 - 283	3e-15	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR5	PF25175	56 - 267	1.6e-30	WDR5 beta-propeller domain
WD40_CDC20-Fz	PF24807	56 - 204	3e-09	CDC20/Fizzy WD40 domain
WD40_Gbeta	PF25391	103 - 266	7.6e-11	G protein beta WD-40 repeat protein
WD40_MABP1-WDR62_2nd	PF24782	107 - 353	1.3e-10	MABP1/WDR62 second WD40 domain
Beta-prop_WDR3_2nd	PF25172	127 - 266	4.5e-10	WDR3 second beta-propeller domain
WD40_Prp19	PF24814	130 - 268	1.5e-15	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	140 - 265	6.4e-06	WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2	PF23414	149 - 265	6.3e-08	Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st	PF24817	161 - 354	5.2e-12	WDHD1 first WD40 domain
WDR55	PF24796	180 - 392	4.9e-11	WDR55
Beta-prop_CAF1B_HIR1	PF24105	181 - 268	1.1e-06	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	182 - 266	1.2e-09	WDR3 first beta-propeller domain
EIF3I	PF24805	183 - 266	5.3e-09	EIF3I
Beta-prop_IFT122_1st	PF23381	184 - 263	2.7e-06	IFT122 first beta-propeller
Beta-prop_WDR5	PF25175	185 - 356	2.5e-17	WDR5 beta-propeller domain
Beta-prop_THOC3	PF25174	188 - 267	2.4e-11	THOC3 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	221 - 378	4.2e-09	TEP-1 second beta-propeller
WD40	PF00400	231 - 261	1.6e-07	WD domain, G-beta repeat
WD40_Prp19	PF24814	325 - 520	1.4e-06	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_1st	PF25171	367 - 605	2.8e-07	WDR36/Utp21 first beta-propeller
WD40_WDHD1_1st	PF24817	373 - 526	1.9e-09	WDHD1 first WD40 domain
Beta-prop_WDR3_2nd	PF25172	417 - 565	3e-07	WDR3 second beta-propeller domain
Beta-prop_THOC3	PF25174	424 - 607	2.3e-15	THOC3 beta-propeller domain
Beta-prop_EML_2	PF23414	428 - 565	2.7e-13	Echinoderm microtubule-associated protein second beta-propeller
WD40_CDC20-Fz	PF24807	440 - 547	1e-09	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	444 - 524	2.6e-08	WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	449 - 607	7.3e-06	WDR36/Utp21 second beta-propeller domain
WD40_Prp19	PF24814	450 - 607	2.7e-10	Prp19 WD40 domain
Beta-prop_WDR5	PF25175	450 - 606	7.6e-19	WDR5 beta-propeller domain
WD40_Gbeta	PF25391	451 - 604	8.4e-06	G protein beta WD-40 repeat protein
Beta-prop_CAF1B_HIR1	PF24105	466 - 605	5.8e-08	CAF1B/HIR1 beta-propeller domain
WD40_CDC20-Fz	PF24807	525 - 607	8e-06	CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd	PF25047	530 - 607	1.9e-06	TEP-1 second beta-propeller
Beta-prop_WDR3_1st	PF25173	533 - 609	2.3e-09	WDR3 first beta-propeller domain
WD40	PF00400	534 - 562	1.5e-06	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013291)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G01330 AT3G18060
fragaria_vesca	FvH4_5g17110
malus_domestica	MD06G1229500.v1.1 MD14G1234400.v1.1
prunus_persica	Prupe.5G230600_v2.0.a1 Prupe.5G230600_v2.0.a1 Prupe.5G230600_v2.0.a1
pyrus_communis	pycom06g20050 pycom14g19520
rosa_chinensis	RchiOBHm_Chr7g0178701
rosa_laevigata	RLG00000005421
rosa_multiflora	Rmu_sc0002320.1_g000019
rosa_roxburghii	Rroxscaffold_3G00274660
rosa_rugosa	Rorug06G0418700
rosa_samantha	Rh7AG018100 Rh7BG017600
rosa_wichuraiana	Rw7G001460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	234
Acc65I	GGTACC	1 cut(s)	257
AccB1I	GGYRCC	1 cut(s)	257
AccI	GTMKAC	1 cut(s)	1692
AccII	CGCG	5 cut(s)	51, 221, 387, 389, 1609
AccIII	TCCGGA	2 cut(s)	236, 965
AciI	CCGC	8 cut(s)	51, 225, 530, 662, 684, 827, 1186, 1826
AclI	AACGTT	1 cut(s)	821
AclWI	GGATC	7 cut(s)	108, 224, 317, 353, 567, 844, 1666
AcoI	YGGCCR	2 cut(s)	52, 694
AcsI	RAATTY	4 cut(s)	514, 539, 568, 1102
AcuI	CTGAAG	1 cut(s)	833
AcvI	CACGTG	1 cut(s)	1671
AcyI	GRCGYC	1 cut(s)	231
AdeI	CACNNNGTG	1 cut(s)	1777
AfaI	GTAC	5 cut(s)	259, 1363, 1598, 1714, 1748
AfiI	CCNNNNNNNGG	3 cut(s)	336, 833, 839
AflII	CTTAAG	2 cut(s)	1445, 1583
AflIII	ACRYGT	2 cut(s)	1668, 1670
AgsI	TTSAA	7 cut(s)	280, 292, 538, 544, 1064, 1284, 1537
AjnI	CCWGG	1 cut(s)	832
AjuI	GAANNNNNNNTTGG	2 cut(s)	1377, 1409
Alw21I	GWGCWC	2 cut(s)	171, 1773
Alw26I	GTCTC	5 cut(s)	238, 466, 555, 1049, 1635
AlwI	GGATC	7 cut(s)	108, 224, 317, 353, 567, 844, 1666
AlwNI	CAGNNNCTG	1 cut(s)	748
Ama87I	CYCGRG	2 cut(s)	301, 1569
Aor13HI	TCCGGA	2 cut(s)	236, 965
AoxI	GGCC	4 cut(s)	21, 52, 694, 1478
ApaI	GGGCCC	1 cut(s)	1482
ApeKI	GCWGC	3 cut(s)	705, 1032, 1124
ApoI	RAATTY	4 cut(s)	514, 539, 568, 1102
ArsI	GACNNNNNNTTYG	2 cut(s)	1310, 1342
Asp700I	GAANNNNTTC	2 cut(s)	287, 377
Asp718I	GGTACC	1 cut(s)	257
AspLEI	GCGC	3 cut(s)	51, 389, 1609
AspS9I	GGNCC	6 cut(s)	295, 327, 527, 1478, 1479, 1558
AsuHPI	GGTGA	5 cut(s)	7, 523, 1362, 1448, 1802
AsuII	TTCGAA	2 cut(s)	481, 566
AvaI	CYCGRG	2 cut(s)	301, 1569
AvaII	GGWCC	4 cut(s)	295, 327, 527, 1558
BaeGI	GKGCMC	2 cut(s)	435, 1482
BalI	TGGCCA	1 cut(s)	696
BanI	GGYRCC	1 cut(s)	257
BanII	GRGCYC	1 cut(s)	1482
BbrPI	CACGTG	1 cut(s)	1671
Bbv12I	GWGCWC	2 cut(s)	171, 1773
BbvI	GCAGC	3 cut(s)	717, 1044, 1136
BccI	CCATC	9 cut(s)	422, 593, 644, 686, 728, 1034, 1370, 1397, 1639
BceAI	ACGGC	5 cut(s)	39, 140, 223, 1695, 1765
BcgI	CGANNNNNNTGC	2 cut(s)	1599, 1633
BciT130I	CCWGG	1 cut(s)	834
BclI	TGATCA	2 cut(s)	883, 1192
BcoDI	GTCTC	5 cut(s)	238, 466, 555, 1049, 1635
BfaI	CTAG	3 cut(s)	192, 860, 1029
BfrI	CTTAAG	2 cut(s)	1445, 1583
BglI	GCCNNNNNGGC	1 cut(s)	702
BisI	GCNGC	5 cut(s)	52, 706, 827, 1033, 1125
BlpI	GCTNAGC	1 cut(s)	1753
BlsI	GCNGC	5 cut(s)	53, 707, 828, 1034, 1126
Bme1390I	CCNGG	1 cut(s)	834
Bme18I	GGWCC	4 cut(s)	295, 327, 527, 1558
BmeT110I	CYCGRG	2 cut(s)	301, 1569
BmgT120I	GGNCC	6 cut(s)	295, 327, 527, 1478, 1479, 1558
BmiI	GGNNCC	6 cut(s)	241, 259, 329, 1479, 1480, 1559
BmrFI	CCNGG	1 cut(s)	834
BmsI	GCATC	4 cut(s)	1195, 1480, 1532, 1786
BplI	GAGNNNNNCTC	5 cut(s)	1165, 1197, 1167, 1199, 1807
BpmI	CTGGAG	5 cut(s)	119, 305, 745, 1314, 1686
Bpu1102I	GCTNAGC	1 cut(s)	1753
Bpu14I	TTCGAA	2 cut(s)	481, 566
BsaAI	YACGTR	1 cut(s)	1671
BsaBI	GATNNNNATC	1 cut(s)	65
BsaHI	GRCGYC	1 cut(s)	231
BsaI	GGTCTC	3 cut(s)	466, 1049, 1635
BsaJI	CCNNGG	4 cut(s)	18, 55, 1309, 1339
BsaWI	WCCGGW	3 cut(s)	236, 965, 1372
Bsc4I	CCNNNNNNNGG	3 cut(s)	336, 833, 839
Bse1I	ACTGG	2 cut(s)	322, 1660
Bse8I	GATNNNNATC	1 cut(s)	65
BseAI	TCCGGA	2 cut(s)	236, 965
BseBI	CCWGG	1 cut(s)	834
BseDI	CCNNGG	4 cut(s)	18, 55, 1309, 1339
BseGI	GGATG	3 cut(s)	856, 1381, 1801
BseJI	GATNNNNATC	1 cut(s)	65
BseLI	CCNNNNNNNGG	3 cut(s)	336, 833, 839
BseMII	CTCAG	5 cut(s)	568, 639, 783, 942, 1121
BseNI	ACTGG	2 cut(s)	322, 1660
BseRI	GAGGAG	1 cut(s)	86
BseSI	GKGCMC	2 cut(s)	435, 1482
BseX3I	CGGCCG	1 cut(s)	52
BseXI	GCAGC	3 cut(s)	717, 1044, 1136
BseYI	CCCAGC	1 cut(s)	9
Bsh1236I	CGCG	5 cut(s)	51, 221, 387, 389, 1609
Bsh1285I	CGRYCG	1 cut(s)	55
BshFI	GGCC	4 cut(s)	23, 54, 696, 1480
BshNI	GGYRCC	1 cut(s)	257
BsiEI	CGRYCG	1 cut(s)	55
BsiHKAI	GWGCWC	2 cut(s)	171, 1773
BsiHKCI	CYCGRG	2 cut(s)	301, 1569
BsiSI	CCGG	5 cut(s)	72, 237, 357, 966, 1373
BslFI	GGGAC	3 cut(s)	20, 313, 1571
BslI	CCNNNNNNNGG	3 cut(s)	336, 833, 839
BsmAI	GTCTC	5 cut(s)	238, 466, 555, 1049, 1635
BsmBI	CGTCTC	1 cut(s)	238
BsmFI	GGGAC	3 cut(s)	20, 313, 1571
BsnI	GGCC	4 cut(s)	23, 54, 696, 1480
Bso31I	GGTCTC	3 cut(s)	466, 1049, 1635
BsoBI	CYCGRG	2 cut(s)	301, 1569
Bsp119I	TTCGAA	2 cut(s)	481, 566
Bsp120I	GGGCCC	1 cut(s)	1478
Bsp1286I	GDGCHC	4 cut(s)	171, 435, 1482, 1773
Bsp13I	TCCGGA	2 cut(s)	236, 965
Bsp1720I	GCTNAGC	1 cut(s)	1753
Bsp19I	CCATGG	1 cut(s)	1309
BspACI	CCGC	8 cut(s)	51, 225, 530, 662, 684, 827, 1186, 1826
BspANI	GGCC	4 cut(s)	23, 54, 696, 1480
BspCNI	CTCAG	5 cut(s)	567, 638, 784, 941, 1120
BspEI	TCCGGA	2 cut(s)	236, 965
BspFNI	CGCG	5 cut(s)	51, 221, 387, 389, 1609
BspHI	TCATGA	1 cut(s)	126
BspLI	GGNNCC	6 cut(s)	241, 259, 329, 1479, 1480, 1559
BspPI	GGATC	7 cut(s)	108, 224, 317, 353, 567, 844, 1666
BspT104I	TTCGAA	2 cut(s)	481, 566
BspT107I	GGYRCC	1 cut(s)	257
BspTI	CTTAAG	2 cut(s)	1445, 1583
BspTNI	GGTCTC	3 cut(s)	466, 1049, 1635
BsrI	ACTGG	2 cut(s)	322, 1660
BssECI	CCNNGG	4 cut(s)	18, 55, 1309, 1339
BssNI	GRCGYC	1 cut(s)	231
BssT1I	CCWWGG	2 cut(s)	1309, 1339
Bst2UI	CCWGG	1 cut(s)	834
Bst6I	CTCTTC	1 cut(s)	1446
BstACI	GRCGYC	1 cut(s)	231
BstAFI	CTTAAG	2 cut(s)	1445, 1583
BstBAI	YACGTR	1 cut(s)	1671
BstBI	TTCGAA	2 cut(s)	481, 566
BstC8I	GCNNGC	4 cut(s)	31, 171, 934, 1708
BstDEI	CTNAG	7 cut(s)	554, 625, 719, 792, 928, 1107, 1753
BstDSI	CCRYGG	2 cut(s)	55, 1309
BstF5I	GGATG	3 cut(s)	856, 1381, 1801
BstFNI	CGCG	5 cut(s)	51, 221, 387, 389, 1609
BstHHI	GCGC	3 cut(s)	51, 389, 1609
BstMAI	GTCTC	5 cut(s)	238, 466, 555, 1049, 1635
BstMCI	CGRYCG	1 cut(s)	55
BstMWI	GCNNNNNNNGC	5 cut(s)	161, 170, 439, 702, 1477
BstNI	CCWGG	1 cut(s)	834
BstNSI	RCATGY	3 cut(s)	859, 1362, 1594
BstSCI	CCNGG	1 cut(s)	832
BstSLI	GKGCMC	2 cut(s)	435, 1482
BstUI	CGCG	5 cut(s)	51, 221, 387, 389, 1609
BstV1I	GCAGC	3 cut(s)	717, 1044, 1136
BstX2I	RGATCY	2 cut(s)	113, 1658
BstYI	RGATCY	2 cut(s)	113, 1658
BstZI	CGGCCG	1 cut(s)	52
BsuRI	GGCC	4 cut(s)	23, 54, 696, 1480
BtgI	CCRYGG	2 cut(s)	55, 1309
BtsCI	GGATG	3 cut(s)	856, 1381, 1801
BtsI	GCAGTG	1 cut(s)	1195
BtsIMutI	CAGTG	6 cut(s)	183, 329, 615, 1195, 1347, 1773
Cac8I	GCNNGC	4 cut(s)	31, 171, 934, 1708
CaiI	CAGNNNCTG	1 cut(s)	748
CciI	TCATGA	1 cut(s)	126
CfoI	GCGC	3 cut(s)	51, 389, 1609
Cfr13I	GGNCC	6 cut(s)	295, 327, 527, 1478, 1479, 1558
CseI	GACGC	2 cut(s)	22, 210
Csp6I	GTAC	5 cut(s)	258, 1362, 1597, 1713, 1747
CviAII	CATG	9 cut(s)	127, 394, 856, 973, 1149, 1303, 1310, 1359, 1591
CviQI	GTAC	5 cut(s)	258, 1362, 1597, 1713, 1747
DdeI	CTNAG	7 cut(s)	554, 625, 719, 792, 928, 1107, 1753
DraIII	CACNNNGTG	1 cut(s)	1777
EaeI	YGGCCR	2 cut(s)	52, 694
EagI	CGGCCG	1 cut(s)	52
Eam1104I	CTCTTC	1 cut(s)	1446
EarI	CTCTTC	1 cut(s)	1446
EciI	GGCGGA	2 cut(s)	214, 673
EclXI	CGGCCG	1 cut(s)	52
Eco130I	CCWWGG	2 cut(s)	1309, 1339
Eco147I	AGGCCT	1 cut(s)	23
Eco24I	GRGCYC	1 cut(s)	1482
Eco31I	GGTCTC	3 cut(s)	466, 1049, 1635
Eco32I	GATATC	2 cut(s)	789, 963
Eco47I	GGWCC	4 cut(s)	295, 327, 527, 1558
Eco52I	CGGCCG	1 cut(s)	52
Eco57I	CTGAAG	1 cut(s)	833
Eco72I	CACGTG	1 cut(s)	1671
Eco88I	CYCGRG	2 cut(s)	301, 1569
EcoO109I	RGGNCCY	2 cut(s)	295, 1478
EcoRI	GAATTC	1 cut(s)	1102
EcoRII	CCWGG	1 cut(s)	832
EcoRV	GATATC	2 cut(s)	789, 963
EcoT14I	CCWWGG	2 cut(s)	1309, 1339
EcoT22I	ATGCAT	1 cut(s)	1801
EcoT38I	GRGCYC	1 cut(s)	1482
ErhI	CCWWGG	2 cut(s)	1309, 1339
Esp3I	CGTCTC	1 cut(s)	238
FaeI	CATG	9 cut(s)	130, 397, 859, 976, 1152, 1306, 1313, 1362, 1594
FaqI	GGGAC	3 cut(s)	20, 313, 1571
FatI	CATG	9 cut(s)	126, 393, 855, 972, 1148, 1302, 1309, 1358, 1590
FauNDI	CATATG	1 cut(s)	1419
FbaI	TGATCA	2 cut(s)	883, 1192
FblI	GTMKAC	1 cut(s)	1692
Fnu4HI	GCNGC	5 cut(s)	52, 706, 827, 1033, 1125
FokI	GGATG	3 cut(s)	863, 1388, 1808
FriOI	GRGCYC	1 cut(s)	1482
Fsp4HI	GCNGC	5 cut(s)	52, 706, 827, 1033, 1125
FspBI	CTAG	3 cut(s)	192, 860, 1029
GlaI	GCGC	3 cut(s)	50, 388, 1608
GluI	GCNGC	5 cut(s)	52, 706, 827, 1033, 1125
GsaI	CCCAGC	1 cut(s)	13
GsuI	CTGGAG	5 cut(s)	119, 305, 745, 1314, 1686
HaeIII	GGCC	4 cut(s)	23, 54, 696, 1480
HapII	CCGG	5 cut(s)	72, 237, 357, 966, 1373
HgaI	GACGC	2 cut(s)	22, 210
HhaI	GCGC	3 cut(s)	51, 389, 1609
Hin1I	GRCGYC	1 cut(s)	231
Hin1II	CATG	9 cut(s)	130, 397, 859, 976, 1152, 1306, 1313, 1362, 1594
Hin6I	GCGC	3 cut(s)	49, 387, 1607
HinP1I	GCGC	3 cut(s)	49, 387, 1607
HincII	GTYRAC	5 cut(s)	42, 1132, 1329, 1693, 1821
HindII	GTYRAC	5 cut(s)	42, 1132, 1329, 1693, 1821
HindIII	AAGCTT	1 cut(s)	1580
HinfI	GANTC	8 cut(s)	61, 401, 483, 727, 1060, 1289, 1344, 1822
HpaII	CCGG	5 cut(s)	72, 237, 357, 966, 1373
HphI	GGTGA	5 cut(s)	7, 523, 1362, 1448, 1802
Hpy166II	GTNNAC	9 cut(s)	42, 160, 740, 1132, 1329, 1412, 1693, 1747, 1821
Hpy188I	TCNGA	9 cut(s)	66, 88, 309, 628, 674, 750, 793, 908, 1110
Hpy8I	GTNNAC	9 cut(s)	42, 160, 740, 1132, 1329, 1412, 1693, 1747, 1821
Hpy99I	CGWCG	4 cut(s)	152, 233, 317, 472
HpyAV	CCTTC	3 cut(s)	518, 1090, 1265
HpyCH4IV	ACGT	4 cut(s)	150, 231, 821, 1670
HpyCH4V	TGCA	7 cut(s)	1208, 1358, 1417, 1523, 1652, 1730, 1799
HpyF10VI	GCNNNNNNNGC	5 cut(s)	161, 170, 439, 702, 1477
HpyF3I	CTNAG	7 cut(s)	554, 625, 719, 792, 928, 1107, 1753
HpySE526I	ACGT	4 cut(s)	150, 231, 821, 1670
Hsp92I	GRCGYC	1 cut(s)	231
Hsp92II	CATG	9 cut(s)	130, 397, 859, 976, 1152, 1306, 1313, 1362, 1594
HspAI	GCGC	3 cut(s)	49, 387, 1607
Kpn2I	TCCGGA	2 cut(s)	236, 965
KpnI	GGTACC	1 cut(s)	261
Ksp22I	TGATCA	2 cut(s)	883, 1192
LmnI	GCTCC	2 cut(s)	99, 1311
Lsp1109I	GCAGC	3 cut(s)	717, 1044, 1136
LweI	GCATC	4 cut(s)	1195, 1480, 1532, 1786
MaeI	CTAG	3 cut(s)	192, 860, 1029
MaeII	ACGT	4 cut(s)	150, 231, 821, 1670
MaeIII	GTNAC	7 cut(s)	181, 487, 892, 982, 1086, 1351, 1429
MboII	GAAGA	7 cut(s)	292, 679, 701, 826, 988, 1148, 1463
MflI	RGATCY	2 cut(s)	113, 1658
MhlI	GDGCHC	4 cut(s)	171, 435, 1482, 1773
MlsI	TGGCCA	1 cut(s)	696
MluCI	AATT	6 cut(s)	120, 514, 539, 568, 577, 1102
MluNI	TGGCCA	1 cut(s)	696
MlyI	GAGTC	2 cut(s)	736, 1816
MmeI	TCCRAC	3 cut(s)	111, 287, 1204
Mox20I	TGGCCA	1 cut(s)	696
Mph1103I	ATGCAT	1 cut(s)	1801
MroI	TCCGGA	2 cut(s)	236, 965
MroXI	GAANNNNTTC	2 cut(s)	287, 377
MscI	TGGCCA	1 cut(s)	696
MseI	TTAA	7 cut(s)	449, 462, 1053, 1335, 1446, 1584, 1722
Msp20I	TGGCCA	1 cut(s)	696
MspA1I	CMGCKG	1 cut(s)	1127
MspCI	CTTAAG	2 cut(s)	1445, 1583
MspI	CCGG	5 cut(s)	72, 237, 357, 966, 1373
MspR9I	CCNGG	1 cut(s)	834
MvaI	CCWGG	1 cut(s)	834
MvnI	CGCG	5 cut(s)	51, 221, 387, 389, 1609
MwoI	GCNNNNNNNGC	5 cut(s)	161, 170, 439, 702, 1477
NcoI	CCATGG	1 cut(s)	1309
NdeI	CATATG	1 cut(s)	1419
NlaIII	CATG	9 cut(s)	130, 397, 859, 976, 1152, 1306, 1313, 1362, 1594
NlaIV	GGNNCC	6 cut(s)	241, 259, 329, 1479, 1480, 1559
NmeAIII	GCCGAG	1 cut(s)	190
NmuCI	GTSAC	3 cut(s)	181, 487, 1351
NsiI	ATGCAT	1 cut(s)	1801
NspI	RCATGY	3 cut(s)	859, 1362, 1594
NspV	TTCGAA	2 cut(s)	481, 566
PaeR7I	CTCGAG	1 cut(s)	1569
PagI	TCATGA	1 cut(s)	126
PceI	AGGCCT	1 cut(s)	23
PcsI	WCGNNNNNNNCGW	4 cut(s)	153, 177, 225, 1140
PdmI	GAANNNNTTC	2 cut(s)	287, 377
PfeI	GAWTC	6 cut(s)	61, 401, 483, 1060, 1289, 1344
PflFI	GACNNNGTC	1 cut(s)	754
PfoI	TCCNGGA	1 cut(s)	832
PkrI	GCNGC	5 cut(s)	53, 707, 828, 1034, 1126
PleI	GAGTC	2 cut(s)	735, 1816
PmaCI	CACGTG	1 cut(s)	1671
PmlI	CACGTG	1 cut(s)	1671
PpsI	GAGTC	2 cut(s)	735, 1816
Ppu21I	YACGTR	1 cut(s)	1671
PpuMI	RGGWCCY	1 cut(s)	295
Psp1406I	AACGTT	1 cut(s)	821
Psp5II	RGGWCCY	1 cut(s)	295
Psp6I	CCWGG	1 cut(s)	832
PspCI	CACGTG	1 cut(s)	1671
PspFI	CCCAGC	1 cut(s)	9
PspGI	CCWGG	1 cut(s)	832
PspN4I	GGNNCC	6 cut(s)	241, 259, 329, 1479, 1480, 1559
PspOMI	GGGCCC	1 cut(s)	1478
PspPI	GGNCC	6 cut(s)	295, 327, 527, 1478, 1479, 1558
PspPPI	RGGWCCY	1 cut(s)	295
PsrI	GAACNNNNNNTAC	2 cut(s)	1580, 1612
PstNI	CAGNNNCTG	1 cut(s)	748
PsuI	RGATCY	2 cut(s)	113, 1658
PsyI	GACNNNGTC	1 cut(s)	754
PvuII	CAGCTG	1 cut(s)	1127
RsaI	GTAC	5 cut(s)	259, 1363, 1598, 1714, 1748
RsaNI	GTAC	5 cut(s)	258, 1362, 1597, 1713, 1747
SalI	GTCGAC	1 cut(s)	1691
SaqAI	TTAA	7 cut(s)	449, 462, 1053, 1335, 1446, 1584, 1722
SatI	GCNGC	5 cut(s)	52, 706, 827, 1033, 1125
Sau96I	GGNCC	6 cut(s)	295, 327, 527, 1478, 1479, 1558
SchI	GAGTC	2 cut(s)	736, 1816
ScrFI	CCNGG	1 cut(s)	834
SduI	GDGCHC	4 cut(s)	171, 435, 1482, 1773
SfaNI	GCATC	4 cut(s)	1195, 1480, 1532, 1786
Sfr274I	CTCGAG	1 cut(s)	1569
SfuI	TTCGAA	2 cut(s)	481, 566
SinI	GGWCC	4 cut(s)	295, 327, 527, 1558
SlaI	CTCGAG	1 cut(s)	1569
SmlI	CTYRAG	3 cut(s)	1445, 1569, 1583
SmoI	CTYRAG	3 cut(s)	1445, 1569, 1583
Sse9I	AATT	6 cut(s)	120, 514, 539, 568, 577, 1102
SseBI	AGGCCT	1 cut(s)	23
SsiI	CCGC	8 cut(s)	51, 225, 530, 662, 684, 827, 1186, 1826
SspMI	CTAG	3 cut(s)	192, 860, 1029
StuI	AGGCCT	1 cut(s)	23
StyD4I	CCNGG	1 cut(s)	832
StyI	CCWWGG	2 cut(s)	1309, 1339
TaiI	ACGT	4 cut(s)	153, 234, 824, 1673
TaqI	TCGA	7 cut(s)	147, 312, 437, 481, 566, 1570, 1692
TasI	AATT	6 cut(s)	120, 514, 539, 568, 577, 1102
TatI	WGTACW	1 cut(s)	1361
TauI	GCSGC	2 cut(s)	54, 829
TfiI	GAWTC	6 cut(s)	61, 401, 483, 1060, 1289, 1344
Tru1I	TTAA	7 cut(s)	449, 462, 1053, 1335, 1446, 1584, 1722
Tru9I	TTAA	7 cut(s)	449, 462, 1053, 1335, 1446, 1584, 1722
TscAI	CASTG	6 cut(s)	190, 329, 622, 1195, 1354, 1780
TseFI	GTSAC	3 cut(s)	181, 487, 1351
TseI	GCWGC	3 cut(s)	705, 1032, 1124
Tsp45I	GTSAC	3 cut(s)	181, 487, 1351
TspDTI	ATGAA	6 cut(s)	382, 904, 978, 989, 1137, 1750
TspGWI	ACGGA	1 cut(s)	106
TspRI	CASTG	6 cut(s)	190, 329, 622, 1195, 1354, 1780
Tth111I	GACNNNGTC	1 cut(s)	754
Vha464I	CTTAAG	2 cut(s)	1445, 1583
VpaK11BI	GGWCC	4 cut(s)	295, 327, 527, 1558
XapI	RAATTY	4 cut(s)	514, 539, 568, 1102
XceI	RCATGY	3 cut(s)	859, 1362, 1594
XhoI	CTCGAG	1 cut(s)	1569
XmiI	GTMKAC	1 cut(s)	1692
XmnI	GAANNNNTTC	2 cut(s)	287, 377
XspI	CTAG	3 cut(s)	192, 860, 1029
ZraI	GACGTC	1 cut(s)	232
Zsp2I	ATGCAT	1 cut(s)	1801

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.