Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0013291

Physical Location & Seq

Genomic Coordinates Reverse (-)

31416354 .. 31419863

Gene Structure 3510 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1234400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1971 bp

ATGATGAACATATGTGATTGGAAGATCTTTAGAATTCTCGGAAATCTCTCCCTCGTCACTCTATTCGTACCCTCCCACCACCTATATATACCGCGGGCTTTCAAATCCCCACTTCTCATTTTTCCCAAAATTCCAAAAATGACCCCCAAGCTCACCGAAGCCTACGCCTGCGTCCCGTCAACGGAGCGAGGCCGTGGGATTCTGATCTCCGGCGACCCAAAATCCGACAAGCTCCTCTACACCAATGGCAGATCCGTAATCATCATGAGCCTCCGGAACCCCCTCGACGTCGCCGTTTACGCCGAGCATGCCTACCCCGTCACCGTCGCTAGGTTCTCCCCCAACGGCGAGTGGATCGCGTCCGCCGACGTCTCCGGCACCGTCAGGATCTGGGGGACCCACAACGACTTCGTGTTGAAGAACGAGTTCAAGGTCCTTTCGGGTCGGATCGACGACCTCCAGTGGTCCCCCGACGGATTGAGGATCGTTGCTTCCGGCGAGGGCAAAGGAAAATCCTTCGTTCGCGCGTTCATGTGGGATTCGGGCTCAACAGTTGGGGATTTTGATGGGCACTCGAAGCGAGTGTTAAGCTGCGCGTTTAAGCCGACGAGGCCCTTTCGAATCGTTACTTGTGGAGAGGATTTCTTGGTGAATTTCTACGAAGGACCGCCTTTCAAATTCAAGCTGTCTCTCAGGGATCATTCAAATTTCGTGAATTGTATAAGGTTCTCTCCAGATGGTAGTAAGTTTATCACTGTAAGCTCAGATAAAAGTGGTATTGTTTATGATGGAAAGACTGCGGATAAGATCGGAGAGCTGTCCTCGGAAGATGGCCACAAAGGCAGTATTTATGCTCTTAGCTGGAGTCCTGATGGTAAACAGGTTCTGACTGTGTCTGCTGACAAGTCGGCAAAAGTATGGGAGATGTCTGAGGATAATAATGGGAAGCTGACGAAAACATTGCCTCCTCCTGGGTCAGGTGGAGTGGATGACATGCTAGTTGGGTGTCTATGGCAGAACGATCATATTGTTACAGTTTCTCTTGGAGGCTTCATTAGTATATTCTCAGCAAGCAACCTTGACAAAGCCCCTCTACTGATATCCGGACACATGAAGAATGTTACTTCATTAGCTGTTCTAAAGAGTAACCCGAAACTTGTATTGTCTAGTAGCTATGATGGTCTTATTGTTAAATGGATTCAAGGTGCTGGATACGGCGGAAAGTTAAAAAGGAAAGAGAATTCTCAAATAAAGTGTTTAGCAGCTGTTGACGAAGAAATCATTACATGTGGATTTGACAATAAGGTTTGGAGAGTTCCTCTCCACAGTGATCAATGTGGAGCTGCAGAGGCTATTGATATTGGAAGTCAGCCAAAGGACATAAGCCTTGCGCTTCTATCTCCTGAACTTGCTTTGGTTTCAACTGATACAGGAGTTGTCATGCTCCGTGGCACGAAAGTTTTATCAACTGTAAACCTTGGGTTCACTGTGACTGCATGTACTATTTCACCAGATGGATGTGAAGCCATTGTTGGTGGGCAGGACGGTAAACTGCATATGTATTCTATAACAGGTGATACACTTAATGAAGAGGCTGTTTTGGAGAAGCATCGGGGACCCATTTCTGTCATCCGTTACTCTCCTGATGTTTCTATGTTTGCATCGGGAGACTTGAACCGAGAAGCTGTCGTCTGGGACCGTGCCTCTCGAGAGATCAAGCTTAAGAACATGCTGTACCATACTGCTCGAATAAACTGCCTTGCTTGGTCTCCTGATAGCACACTGGTTGCAACTGGATCTCTTGACACGTGTGTTATCATATATGAAGTCGACAAGCCTCCAGCAAGCCGTGTAACCATTAAGGGTGCACACTTGGGTGGCGTCTACGGCATAACTTTCACCGATGAGCACACTCTGGTAAGCTCAGGGGAGGATGCATTTGTTCGTGTCTGGAGGTTGACTCCGCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005856 GO:0005884 GO:0005938 GO:0006996 GO:0007010 GO:0007015 GO:0008064 GO:0008092 GO:0008150 GO:0008154 GO:0009987 GO:0010638 GO:0015629 GO:0016043 GO:0022411 GO:0030029 GO:0030036 GO:0030042 GO:0030832 GO:0030834 GO:0030836 GO:0030863 GO:0030864 GO:0031461 GO:0032535 GO:0032956 GO:0032970 GO:0032984 GO:0032991 GO:0033043 GO:0042641 GO:0042643 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043243 GO:0043244 GO:0043624 GO:0043933 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0044877 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051015 GO:0051128 GO:0051130 GO:0051261 GO:0051493 GO:0051495 GO:0065007 GO:0065008 GO:0071840 GO:0071944 GO:0080008 GO:0090066 GO:0097435 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0110053 GO:1901879 GO:1901881 GO:1902494 GO:1902903 GO:1902905 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

657

Amino Acids

71.51

Weight (kDa)

7.55

Isoelectric Point (pI)

27.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Beta-prop_WDR3_1st	PF25173	84 - 220	6.2e-09	WDR3 first beta-propeller domain
Beta-prop_EML	PF23409	100 - 329	4.7e-15	Echinoderm microtubule-associated protein first beta-propeller
WD40_WDHD1_1st	PF24817	101 - 162	5.5e-06	WDHD1 first WD40 domain
Beta-prop_WDR5	PF25175	102 - 313	2.5e-30	WDR5 beta-propeller domain
WD40_CDC20-Fz	PF24807	103 - 249	2.9e-08	CDC20/Fizzy WD40 domain
WD40_Gbeta	PF25391	149 - 312	2.2e-10	G protein beta WD-40 repeat protein
WD40_MABP1-WDR62_2nd	PF24782	153 - 399	6.7e-10	MABP1/WDR62 second WD40 domain
Beta-prop_WDR3_2nd	PF25172	173 - 312	3.4e-10	WDR3 second beta-propeller domain
WD40_Prp19	PF24814	176 - 314	1.9e-15	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	186 - 311	5.4e-06	WDR36/Utp21 second beta-propeller domain
WD40_WDHD1_1st	PF24817	193 - 400	7.2e-14	WDHD1 first WD40 domain
Beta-prop_EML_2	PF23414	195 - 311	1.6e-08	Echinoderm microtubule-associated protein second beta-propeller
WDR55	PF24796	226 - 438	8.6e-13	WDR55
Beta-prop_CAF1B_HIR1	PF24105	227 - 314	1.6e-07	CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	228 - 312	1.8e-10	WDR3 first beta-propeller domain
EIF3I	PF24805	229 - 313	2.1e-08	EIF3I
Beta-prop_IFT122_1st	PF23381	230 - 309	2e-06	IFT122 first beta-propeller
Beta-prop_WDR5	PF25175	231 - 401	1.4e-17	WDR5 beta-propeller domain
Beta-prop_EML	PF23409	234 - 421	1e-07	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_THOC3	PF25174	234 - 314	3.3e-12	THOC3 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	266 - 423	3.7e-09	TEP-1 second beta-propeller
WD40	PF00400	277 - 307	1.3e-07	WD domain, G-beta repeat
WD40_Prp19	PF24814	371 - 566	1.2e-06	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_1st	PF25171	414 - 651	9.6e-08	WDR36/Utp21 first beta-propeller
WD40_WDHD1_1st	PF24817	419 - 572	1.9e-08	WDHD1 first WD40 domain
Beta-prop_EML_2	PF23414	474 - 611	1.5e-12	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st	PF25173	489 - 570	1.6e-08	WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_2nd	PF25168	494 - 654	7.4e-07	WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR5	PF25175	496 - 652	1.2e-19	WDR5 beta-propeller domain
WD40_Prp19	PF24814	496 - 653	2.6e-11	Prp19 WD40 domain
WD40_Gbeta	PF25391	498 - 647	6e-06	G protein beta WD-40 repeat protein
WD40_CDC20-Fz	PF24807	499 - 599	4.6e-10	CDC20/Fizzy WD40 domain
Beta-prop_CAF1B_HIR1	PF24105	512 - 652	1.2e-08	CAF1B/HIR1 beta-propeller domain
WDR55	PF24796	541 - 651	5.1e-06	WDR55
WD40_CDC20-Fz	PF24807	564 - 653	1.1e-08	CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd	PF25047	576 - 653	1.7e-08	TEP-1 second beta-propeller
Beta-prop_WDR3_1st	PF25173	579 - 655	3.9e-10	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	580 - 653	6e-10	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	580 - 655	9.9e-06	WDHD1 first WD40 domain
WD40	PF00400	580 - 608	1.1e-06	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

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Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013291)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G01330 AT3G18060
fragaria_vesca	FvH4_5g17110
malus_domestica	MD06G1229500.v1.1 MD14G1234400.v1.1
prunus_persica	Prupe.5G230600_v2.0.a1 Prupe.5G230600_v2.0.a1 Prupe.5G230600_v2.0.a1
pyrus_communis	pycom06g20050 pycom14g19520
rosa_chinensis	RchiOBHm_Chr7g0178701
rosa_laevigata	RLG00000005421
rosa_multiflora	Rmu_sc0002320.1_g000019
rosa_roxburghii	Rroxscaffold_3G00274660
rosa_rugosa	Rorug06G0418700
rosa_samantha	Rh7AG018100 Rh7BG017600
rosa_wichuraiana	Rw7G001460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	291, 372
AccB1I	GGYRCC	1 cut(s)	377
AccI	GTMKAC	2 cut(s)	1830, 1884
AccII	CGCG	5 cut(s)	94, 359, 525, 527, 596
AccIII	TCCGGA	2 cut(s)	273, 1103
AciI	CCGC	7 cut(s)	92, 94, 363, 668, 800, 1218, 1964
AclWI	GGATC	7 cut(s)	246, 362, 395, 455, 491, 705, 1804
AcoI	YGGCCR	1 cut(s)	832
AcsI	RAATTY	6 cut(s)	33, 129, 652, 677, 706, 1240
AcvI	CACGTG	1 cut(s)	1809
AcyI	GRCGYC	3 cut(s)	288, 369, 1881
AfaI	GTAC	3 cut(s)	69, 1501, 1736
AfiI	CCNNNNNNNGG	4 cut(s)	181, 330, 971, 977
AflII	CTTAAG	1 cut(s)	1721
AflIII	ACRYGT	3 cut(s)	1286, 1806, 1808
AgsI	TTSAA	9 cut(s)	103, 418, 430, 676, 682, 705, 1202, 1422, 1675
AjnI	CCWGG	1 cut(s)	970
AjuI	GAANNNNNNNTTGG	2 cut(s)	1515, 1547
AleI	CACNNNNGTG	1 cut(s)	1875
Alw21I	GWGCWC	2 cut(s)	1870, 1911
Alw26I	GTCTC	4 cut(s)	376, 693, 1662, 1773
Alw44I	GTGCAC	1 cut(s)	1866
AlwI	GGATC	7 cut(s)	246, 362, 395, 455, 491, 705, 1804
AlwNI	CAGNNNCTG	2 cut(s)	390, 886
Ama87I	CYCGRG	1 cut(s)	1707
Aor13HI	TCCGGA	2 cut(s)	273, 1103
AoxI	GGCC	3 cut(s)	190, 611, 832
ApaLI	GTGCAC	1 cut(s)	1866
ApeKI	GCWGC	3 cut(s)	591, 1262, 1343
ApoI	RAATTY	6 cut(s)	33, 129, 652, 677, 706, 1240
Asp700I	GAANNNNTTC	1 cut(s)	425
AspLEI	GCGC	3 cut(s)	527, 596, 1393
AspS9I	GGNCC	7 cut(s)	396, 433, 465, 612, 665, 1616, 1696
AsuHPI	GGTGA	6 cut(s)	145, 313, 661, 1500, 1586, 1891
AsuII	TTCGAA	1 cut(s)	619
AvaI	CYCGRG	1 cut(s)	1707
AvaII	GGWCC	6 cut(s)	396, 433, 465, 665, 1616, 1696
BaeGI	GKGCMC	2 cut(s)	573, 1870
BaeI	ACNNNNGTAYC	2 cut(s)	1419, 1452
BalI	TGGCCA	1 cut(s)	834
BanI	GGYRCC	1 cut(s)	377
BanII	GRGCYC	1 cut(s)	548
BbrPI	CACGTG	1 cut(s)	1809
Bbv12I	GWGCWC	2 cut(s)	1870, 1911
BbvI	GCAGC	3 cut(s)	578, 1274, 1330
BccI	CCATC	7 cut(s)	560, 731, 782, 824, 866, 1172, 1508
BceAI	ACGGC	6 cut(s)	177, 278, 361, 1231, 1833, 1903
BcgI	CGANNNNNNTGC	4 cut(s)	943, 977, 1737, 1771
BciT130I	CCWGG	1 cut(s)	972
BciVI	GTATCC	1 cut(s)	1205
BclI	TGATCA	1 cut(s)	1330
BcoDI	GTCTC	4 cut(s)	376, 693, 1662, 1773
BfaI	CTAG	3 cut(s)	330, 998, 1167
BfmI	CTRYAG	1 cut(s)	1344
BfrI	CTTAAG	1 cut(s)	1721
BfuI	GTATCC	1 cut(s)	1205
BglI	GCCNNNNNGGC	2 cut(s)	610, 840
BglII	AGATCT	1 cut(s)	24
BisI	GCNGC	3 cut(s)	592, 1263, 1344
BlsI	GCNGC	3 cut(s)	593, 1264, 1345
Bme1390I	CCNGG	1 cut(s)	972
Bme18I	GGWCC	6 cut(s)	396, 433, 465, 665, 1616, 1696
BmeT110I	CYCGRG	1 cut(s)	1707
BmgT120I	GGNCC	7 cut(s)	396, 433, 465, 612, 665, 1616, 1696
BmiI	GGNNCC	8 cut(s)	278, 379, 397, 398, 467, 1617, 1618, 1697
BmrFI	CCNGG	1 cut(s)	972
BmsI	GCATC	3 cut(s)	1618, 1670, 1924
BplI	GAGNNNNNCTC	7 cut(s)	806, 838, 1303, 1335, 1305, 1337, 1945
BpmI	CTGGAG	4 cut(s)	443, 717, 883, 1824
Bpu10I	CCTNAGC	1 cut(s)	1924
Bpu14I	TTCGAA	1 cut(s)	619
BsaAI	YACGTR	1 cut(s)	1809
BsaBI	GATNNNNATC	1 cut(s)	203
BsaHI	GRCGYC	3 cut(s)	288, 369, 1881
BsaI	GGTCTC	1 cut(s)	1773
BsaJI	CCNNGG	6 cut(s)	92, 193, 822, 971, 1447, 1477
BsaWI	WCCGGW	2 cut(s)	273, 1103
BsaXI	ACNNNNNCTCC	2 cut(s)	949, 979
Bsc4I	CCNNNNNNNGG	4 cut(s)	181, 330, 971, 977
Bse1I	ACTGG	3 cut(s)	460, 1788, 1798
Bse3DI	GCAATG	1 cut(s)	959
Bse8I	GATNNNNATC	1 cut(s)	203
BseAI	TCCGGA	2 cut(s)	273, 1103
BseBI	CCWGG	1 cut(s)	972
BseDI	CCNNGG	6 cut(s)	92, 193, 822, 971, 1447, 1477
BseGI	GGATG	4 cut(s)	994, 1523, 1629, 1939
BseJI	GATNNNNATC	1 cut(s)	203
BseLI	CCNNNNNNNGG	4 cut(s)	181, 330, 971, 977
BseMI	GCAATG	1 cut(s)	959
BseMII	CTCAG	5 cut(s)	706, 777, 921, 1080, 1938
BseNI	ACTGG	3 cut(s)	460, 1788, 1798
BseRI	GAGGAG	2 cut(s)	224, 957
BseSI	GKGCMC	2 cut(s)	573, 1870
BseXI	GCAGC	3 cut(s)	578, 1274, 1330
Bsh1236I	CGCG	5 cut(s)	94, 359, 525, 527, 596
BshFI	GGCC	3 cut(s)	192, 613, 834
BshNI	GGYRCC	1 cut(s)	377
BsiHKAI	GWGCWC	2 cut(s)	1870, 1911
BsiHKCI	CYCGRG	1 cut(s)	1707
BsiSI	CCGG	5 cut(s)	210, 274, 375, 495, 1104
BslFI	GGGAC	5 cut(s)	158, 409, 451, 1629, 1709
BslI	CCNNNNNNNGG	4 cut(s)	181, 330, 971, 977
BsmAI	GTCTC	4 cut(s)	376, 693, 1662, 1773
BsmBI	CGTCTC	1 cut(s)	376
BsmFI	GGGAC	5 cut(s)	158, 409, 451, 1629, 1709
BsnI	GGCC	3 cut(s)	192, 613, 834
Bso31I	GGTCTC	1 cut(s)	1773
BsoBI	CYCGRG	1 cut(s)	1707
Bsp119I	TTCGAA	1 cut(s)	619
Bsp1286I	GDGCHC	4 cut(s)	548, 573, 1870, 1911
Bsp13I	TCCGGA	2 cut(s)	273, 1103
BspACI	CCGC	7 cut(s)	92, 94, 363, 668, 800, 1218, 1964
BspANI	GGCC	3 cut(s)	192, 613, 834
BspCNI	CTCAG	5 cut(s)	705, 776, 922, 1079, 1937
BspEI	TCCGGA	2 cut(s)	273, 1103
BspFNI	CGCG	5 cut(s)	94, 359, 525, 527, 596
BspHI	TCATGA	1 cut(s)	264
BspLI	GGNNCC	8 cut(s)	278, 379, 397, 398, 467, 1617, 1618, 1697
BspMAI	CTGCAG	1 cut(s)	1348
BspPI	GGATC	7 cut(s)	246, 362, 395, 455, 491, 705, 1804
BspT104I	TTCGAA	1 cut(s)	619
BspT107I	GGYRCC	1 cut(s)	377
BspTI	CTTAAG	1 cut(s)	1721
BspTNI	GGTCTC	1 cut(s)	1773
BsrDI	GCAATG	1 cut(s)	959
BsrI	ACTGG	3 cut(s)	460, 1788, 1798
BssECI	CCNNGG	6 cut(s)	92, 193, 822, 971, 1447, 1477
BssNI	GRCGYC	3 cut(s)	288, 369, 1881
BssT1I	CCWWGG	1 cut(s)	1477
Bst2UI	CCWGG	1 cut(s)	972
Bst6I	CTCTTC	1 cut(s)	1584
BstACI	GRCGYC	3 cut(s)	288, 369, 1881
BstAFI	CTTAAG	1 cut(s)	1721
BstBAI	YACGTR	1 cut(s)	1809
BstBI	TTCGAA	1 cut(s)	619
BstC8I	GCNNGC	5 cut(s)	96, 169, 309, 1072, 1846
BstDEI	CTNAG	6 cut(s)	692, 763, 857, 930, 1066, 1924
BstDSI	CCRYGG	3 cut(s)	92, 193, 1447
BstENI	CCTNNNNNAGG	1 cut(s)	975
BstF5I	GGATG	4 cut(s)	994, 1523, 1629, 1939
BstFNI	CGCG	5 cut(s)	94, 359, 525, 527, 596
BstHHI	GCGC	3 cut(s)	527, 596, 1393
BstMAI	GTCTC	4 cut(s)	376, 693, 1662, 1773
BstMWI	GCNNNNNNNGC	7 cut(s)	299, 308, 577, 610, 840, 1349, 1887
BstNI	CCWGG	1 cut(s)	972
BstNSI	RCATGY	5 cut(s)	311, 997, 1290, 1500, 1732
BstSCI	CCNGG	1 cut(s)	970
BstSFI	CTRYAG	1 cut(s)	1344
BstSLI	GKGCMC	2 cut(s)	573, 1870
BstUI	CGCG	5 cut(s)	94, 359, 525, 527, 596
BstV1I	GCAGC	3 cut(s)	578, 1274, 1330
BstX2I	RGATCY	4 cut(s)	24, 251, 387, 1796
BstYI	RGATCY	4 cut(s)	24, 251, 387, 1796
BsuI	GTATCC	1 cut(s)	1205
BsuRI	GGCC	3 cut(s)	192, 613, 834
BtgI	CCRYGG	3 cut(s)	92, 193, 1447
BtsCI	GGATG	4 cut(s)	994, 1523, 1629, 1939
BtsIMutI	CAGTG	5 cut(s)	467, 753, 1333, 1485, 1781
Cac8I	GCNNGC	5 cut(s)	96, 169, 309, 1072, 1846
CaiI	CAGNNNCTG	2 cut(s)	390, 886
CciI	TCATGA	1 cut(s)	264
CfoI	GCGC	3 cut(s)	527, 596, 1393
Cfr13I	GGNCC	7 cut(s)	396, 433, 465, 612, 665, 1616, 1696
Cfr42I	CCGCGG	1 cut(s)	95
CseI	GACGC	3 cut(s)	160, 348, 1870
Csp6I	GTAC	3 cut(s)	68, 1500, 1735
CviAII	CATG	9 cut(s)	265, 308, 532, 994, 1111, 1287, 1441, 1497, 1729
CviQI	GTAC	3 cut(s)	68, 1500, 1735
DdeI	CTNAG	6 cut(s)	692, 763, 857, 930, 1066, 1924
EaeI	YGGCCR	1 cut(s)	832
Eam1104I	CTCTTC	1 cut(s)	1584
EarI	CTCTTC	1 cut(s)	1584
EciI	GGCGGA	2 cut(s)	352, 1233
Eco130I	CCWWGG	1 cut(s)	1477
Eco24I	GRGCYC	1 cut(s)	548
Eco31I	GGTCTC	1 cut(s)	1773
Eco32I	GATATC	1 cut(s)	1101
Eco47I	GGWCC	6 cut(s)	396, 433, 465, 665, 1616, 1696
Eco72I	CACGTG	1 cut(s)	1809
Eco88I	CYCGRG	1 cut(s)	1707
EcoNI	CCTNNNNNAGG	1 cut(s)	975
EcoO109I	RGGNCCY	4 cut(s)	396, 433, 612, 1616
EcoRI	GAATTC	2 cut(s)	33, 1240
EcoRII	CCWGG	1 cut(s)	970
EcoRV	GATATC	1 cut(s)	1101
EcoT14I	CCWWGG	1 cut(s)	1477
EcoT22I	ATGCAT	1 cut(s)	1939
EcoT38I	GRGCYC	1 cut(s)	548
ErhI	CCWWGG	1 cut(s)	1477
Esp3I	CGTCTC	1 cut(s)	376
FaeI	CATG	9 cut(s)	268, 311, 535, 997, 1114, 1290, 1444, 1500, 1732
FaqI	GGGAC	5 cut(s)	158, 409, 451, 1629, 1709
FatI	CATG	9 cut(s)	264, 307, 531, 993, 1110, 1286, 1440, 1496, 1728
FauI	CCCGC	1 cut(s)	87
FauNDI	CATATG	2 cut(s)	11, 1557
FbaI	TGATCA	1 cut(s)	1330
FblI	GTMKAC	2 cut(s)	1830, 1884
Fnu4HI	GCNGC	3 cut(s)	592, 1263, 1344
FokI	GGATG	4 cut(s)	1001, 1530, 1616, 1946
FriOI	GRGCYC	1 cut(s)	548
Fsp4HI	GCNGC	3 cut(s)	592, 1263, 1344
FspBI	CTAG	3 cut(s)	330, 998, 1167
GlaI	GCGC	3 cut(s)	526, 595, 1392
GluI	GCNGC	3 cut(s)	592, 1263, 1344
GsuI	CTGGAG	4 cut(s)	443, 717, 883, 1824
HaeIII	GGCC	3 cut(s)	192, 613, 834
HapII	CCGG	5 cut(s)	210, 274, 375, 495, 1104
HgaI	GACGC	3 cut(s)	160, 348, 1870
HhaI	GCGC	3 cut(s)	527, 596, 1393
Hin1I	GRCGYC	3 cut(s)	288, 369, 1881
Hin1II	CATG	9 cut(s)	268, 311, 535, 997, 1114, 1290, 1444, 1500, 1732
Hin6I	GCGC	3 cut(s)	525, 594, 1391
HinP1I	GCGC	3 cut(s)	525, 594, 1391
HincII	GTYRAC	4 cut(s)	180, 1270, 1831, 1959
HindII	GTYRAC	4 cut(s)	180, 1270, 1831, 1959
HindIII	AAGCTT	1 cut(s)	1718
HinfI	GANTC	6 cut(s)	199, 539, 621, 865, 1198, 1960
HpaII	CCGG	5 cut(s)	210, 274, 375, 495, 1104
HphI	GGTGA	6 cut(s)	145, 313, 661, 1500, 1586, 1891
Hpy188I	TCNGA	9 cut(s)	41, 204, 226, 447, 766, 812, 826, 888, 931
Hpy99I	CGWCG	7 cut(s)	290, 293, 329, 371, 455, 476, 610
HpyAV	CCTTC	2 cut(s)	526, 656
HpyCH4IV	ACGT	3 cut(s)	288, 369, 1808
HpyCH4V	TGCA	7 cut(s)	1346, 1496, 1555, 1661, 1790, 1868, 1937
HpyF10VI	GCNNNNNNNGC	7 cut(s)	299, 308, 577, 610, 840, 1349, 1887
HpyF3I	CTNAG	6 cut(s)	692, 763, 857, 930, 1066, 1924
HpySE526I	ACGT	3 cut(s)	288, 369, 1808
Hsp92I	GRCGYC	3 cut(s)	288, 369, 1881
Hsp92II	CATG	9 cut(s)	268, 311, 535, 997, 1114, 1290, 1444, 1500, 1732
HspAI	GCGC	3 cut(s)	525, 594, 1391
KflI	GGGWCCC	2 cut(s)	396, 1616
Kpn2I	TCCGGA	2 cut(s)	273, 1103
Ksp22I	TGATCA	1 cut(s)	1330
KspI	CCGCGG	1 cut(s)	95
LmnI	GCTCC	4 cut(s)	184, 237, 1340, 1449
Lsp1109I	GCAGC	3 cut(s)	578, 1274, 1330
LweI	GCATC	3 cut(s)	1618, 1670, 1924
MaeI	CTAG	3 cut(s)	330, 998, 1167
MaeII	ACGT	3 cut(s)	288, 369, 1808
MaeIII	GTNAC	9 cut(s)	55, 319, 625, 1030, 1120, 1145, 1489, 1634, 1852
MboII	GAAGA	6 cut(s)	34, 430, 839, 1126, 1286, 1601
MflI	RGATCY	4 cut(s)	24, 251, 387, 1796
MhlI	GDGCHC	4 cut(s)	548, 573, 1870, 1911
MlsI	TGGCCA	1 cut(s)	834
MluCI	AATT	7 cut(s)	33, 129, 652, 677, 706, 715, 1240
MluNI	TGGCCA	1 cut(s)	834
MlyI	GAGTC	2 cut(s)	874, 1954
MmeI	TCCRAC	2 cut(s)	249, 425
Mox20I	TGGCCA	1 cut(s)	834
Mph1103I	ATGCAT	1 cut(s)	1939
MroI	TCCGGA	2 cut(s)	273, 1103
MroXI	GAANNNNTTC	1 cut(s)	425
MscI	TGGCCA	1 cut(s)	834
MseI	TTAA	7 cut(s)	587, 600, 1191, 1226, 1584, 1722, 1860
MslI	CAYNNNNRTG	1 cut(s)	1875
Msp20I	TGGCCA	1 cut(s)	834
MspA1I	CMGCKG	2 cut(s)	94, 1265
MspCI	CTTAAG	1 cut(s)	1721
MspI	CCGG	5 cut(s)	210, 274, 375, 495, 1104
MspR9I	CCNGG	1 cut(s)	972
MvaI	CCWGG	1 cut(s)	972
MvnI	CGCG	5 cut(s)	94, 359, 525, 527, 596
MwoI	GCNNNNNNNGC	7 cut(s)	299, 308, 577, 610, 840, 1349, 1887
NdeI	CATATG	2 cut(s)	11, 1557
NlaIII	CATG	9 cut(s)	268, 311, 535, 997, 1114, 1290, 1444, 1500, 1732
NlaIV	GGNNCC	8 cut(s)	278, 379, 397, 398, 467, 1617, 1618, 1697
NmeAIII	GCCGAG	1 cut(s)	328
NmuCI	GTSAC	3 cut(s)	55, 319, 1489
NsiI	ATGCAT	1 cut(s)	1939
NspI	RCATGY	5 cut(s)	311, 997, 1290, 1500, 1732
NspV	TTCGAA	1 cut(s)	619
OliI	CACNNNNGTG	1 cut(s)	1875
PaeI	GCATGC	1 cut(s)	311
PaeR7I	CTCGAG	1 cut(s)	1707
PagI	TCATGA	1 cut(s)	264
PciI	ACATGT	1 cut(s)	1286
PcsI	WCGNNNNNNNCGW	2 cut(s)	291, 363
PdmI	GAANNNNTTC	1 cut(s)	425
PfeI	GAWTC	4 cut(s)	199, 539, 621, 1198
PflFI	GACNNNGTC	1 cut(s)	892
PkrI	GCNGC	3 cut(s)	593, 1264, 1345
PleI	GAGTC	2 cut(s)	873, 1954
PmaCI	CACGTG	1 cut(s)	1809
PmlI	CACGTG	1 cut(s)	1809
PpsI	GAGTC	2 cut(s)	873, 1954
Ppu21I	YACGTR	1 cut(s)	1809
PpuMI	RGGWCCY	3 cut(s)	396, 433, 1616
PscI	ACATGT	1 cut(s)	1286
Psp5II	RGGWCCY	3 cut(s)	396, 433, 1616
Psp6I	CCWGG	1 cut(s)	970
PspCI	CACGTG	1 cut(s)	1809
PspGI	CCWGG	1 cut(s)	970
PspN4I	GGNNCC	8 cut(s)	278, 379, 397, 398, 467, 1617, 1618, 1697
PspPI	GGNCC	7 cut(s)	396, 433, 465, 612, 665, 1616, 1696
PspPPI	RGGWCCY	3 cut(s)	396, 433, 1616
PsrI	GAACNNNNNNTAC	2 cut(s)	1718, 1750
PstI	CTGCAG	1 cut(s)	1348
PstNI	CAGNNNCTG	2 cut(s)	390, 886
PsuI	RGATCY	4 cut(s)	24, 251, 387, 1796
PsyI	GACNNNGTC	1 cut(s)	892
PvuII	CAGCTG	1 cut(s)	1265
RsaI	GTAC	3 cut(s)	69, 1501, 1736
RsaNI	GTAC	3 cut(s)	68, 1500, 1735
RseI	CAYNNNNRTG	1 cut(s)	1875
SacII	CCGCGG	1 cut(s)	95
SalI	GTCGAC	1 cut(s)	1829
SaqAI	TTAA	7 cut(s)	587, 600, 1191, 1226, 1584, 1722, 1860
SatI	GCNGC	3 cut(s)	592, 1263, 1344
Sau96I	GGNCC	7 cut(s)	396, 433, 465, 612, 665, 1616, 1696
SchI	GAGTC	2 cut(s)	874, 1954
ScrFI	CCNGG	1 cut(s)	972
SduI	GDGCHC	4 cut(s)	548, 573, 1870, 1911
SfaNI	GCATC	3 cut(s)	1618, 1670, 1924
SfcI	CTRYAG	1 cut(s)	1344
Sfr274I	CTCGAG	1 cut(s)	1707
Sfr303I	CCGCGG	1 cut(s)	95
SfuI	TTCGAA	1 cut(s)	619
SgrBI	CCGCGG	1 cut(s)	95
SinI	GGWCC	6 cut(s)	396, 433, 465, 665, 1616, 1696
SlaI	CTCGAG	1 cut(s)	1707
SmiMI	CAYNNNNRTG	1 cut(s)	1875
SmlI	CTYRAG	2 cut(s)	1707, 1721
SmoI	CTYRAG	2 cut(s)	1707, 1721
SphI	GCATGC	1 cut(s)	311
Sse9I	AATT	7 cut(s)	33, 129, 652, 677, 706, 715, 1240
SsiI	CCGC	7 cut(s)	92, 94, 363, 668, 800, 1218, 1964
SspMI	CTAG	3 cut(s)	330, 998, 1167
StyD4I	CCNGG	1 cut(s)	970
StyI	CCWWGG	1 cut(s)	1477
TaiI	ACGT	3 cut(s)	291, 372, 1811
TaqI	TCGA	7 cut(s)	285, 450, 575, 619, 1708, 1747, 1830
TasI	AATT	7 cut(s)	33, 129, 652, 677, 706, 715, 1240
TatI	WGTACW	1 cut(s)	1499
TfiI	GAWTC	4 cut(s)	199, 539, 621, 1198
Tru1I	TTAA	7 cut(s)	587, 600, 1191, 1226, 1584, 1722, 1860
Tru9I	TTAA	7 cut(s)	587, 600, 1191, 1226, 1584, 1722, 1860
TscAI	CASTG	5 cut(s)	467, 760, 1333, 1492, 1788
TseFI	GTSAC	3 cut(s)	55, 319, 1489
TseI	GCWGC	3 cut(s)	591, 1262, 1343
Tsp45I	GTSAC	3 cut(s)	55, 319, 1489
TspDTI	ATGAA	7 cut(s)	20, 520, 1042, 1116, 1127, 1602, 1839
TspGWI	ACGGA	5 cut(s)	197, 244, 489, 1436, 1622
TspRI	CASTG	5 cut(s)	467, 760, 1333, 1492, 1788
Tth111I	GACNNNGTC	1 cut(s)	892
Vha464I	CTTAAG	1 cut(s)	1721
VneI	GTGCAC	1 cut(s)	1866
VpaK11BI	GGWCC	6 cut(s)	396, 433, 465, 665, 1616, 1696
XagI	CCTNNNNNAGG	1 cut(s)	975
XapI	RAATTY	6 cut(s)	33, 129, 652, 677, 706, 1240
XceI	RCATGY	5 cut(s)	311, 997, 1290, 1500, 1732
XhoI	CTCGAG	1 cut(s)	1707
XmiI	GTMKAC	2 cut(s)	1830, 1884
XmnI	GAANNNNTTC	1 cut(s)	425
XspI	CTAG	3 cut(s)	330, 998, 1167
ZraI	GACGTC	2 cut(s)	289, 370
Zsp2I	ATGCAT	1 cut(s)	1939

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.