Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7B

Orthogroup ID

OG0013291

Physical Location & Seq

Genomic Coordinates Forward (+)

1143578 .. 1146981

Gene Structure 3404 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7BG017600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1830 bp

ATGCCCCAACTCGCCGAGGCCTACGCCTGCGTGCCGTCGACGGAGCGCGGCCGGGGGATCCTGATCTCCGGCGACCCCAAATCCAACCGGCTCCTCTACACCAATGGCAGATCCGTGATCATCATGAACCTCGACAACCCTCTCGACGTCGCCGTCTACGCGGAGCACGCCTACCCGGCCACCGTCGCCAGATTCTCCCCCAACGGCGAGTGGATCGCGTCCGCCGACGTCTCCGGCACCGTCAGGATCTGGGGGACCCACAACGATTTCGTCCTCAAGAACGAGTTCAAGGTCCTCTCGGGTCGGATCGACGACCTCCAGTGGTCCGCCGACGGAATGAGGATCGTCGCCTGCGGCGAAGGCAAAGGGAAGTCGCTTGTCCGCGCCTTCATGTGGGATTCTGGGTCGACTGTGGGGGATTTCGATGGGCATTCGAAACGGGTTTTGAGCTGTTGTTTCAAGCCAACCAGACCCTTTCGCATTGTGACATGCGGTGAGGATTTCCTGGTCAATTTCTATGAAGGGCCTCCTTTCAAATTCAAGCAGTCTCACAGGGATCACTCGAATTTTGTGAACTGTGTAAGGTACTCTCCAGATGGGAGTAAGTTTATCACTGTAAGCTCAGATAAGAAGGGTCTTATTTTTGATGGGAAGACTGCGGAGAAGATCGGAGAGCTTTCATCGGAAGATGGCCACAAAGGCAGCGTTTATGCTCTTAGCTGGAGTCCTGATGGCAAACAGATTCTGACTGTGTCCGCTGACAAGTCGGCGAAAGTATGGGATATATGTGAGGATGGTAATGGGAAGGTGAATAAAACATTGACATGTCCTGGATCAGGTGGAGTGGATGACATGCTTGTTGGCGGCCTATGGCAGAATGATCATATAGTTACTGTTTCACTTGGAGGCATGATCAGTATATTCTCAGCAAGTGACCTTGATAAAGCCCCAATATCGCTATTTGGACACATGAAGAATATTACTTCATTGGCTGTCCTAAAGAATGACCCAAAACTTATATTGTCTACCAGCTATGATGGTCTAATTGTTAAGTGGGTTCAAGGCGTTGGATATAATGGAAAATTACAAAGGAAGGAGACTTCTCAAATAAAATGTTTTGCAGCTGTTGAAGAAGAAATTGTTTCTTCTGGATTTGACAATACGGTGTGGAGAGTTCCATTACAGGGTGATCAATGTGGAGATGCAGAATCTGTTGATGTTGGTAGTCAGCCGAAGGACATAAGCCATGCCCTTCAGTCTCCTGAACTTGTTTTGGTTTCAATTGATTCAGGAGCTGTCTTGCTTCGTGGCAGCAAAGTAGTGTCAACTATTAACCTTGGGTTTACTGTCACAGCATCTGCTATTGCACCAGATGGTAGTGAAGCTATCATTGGTGGGCAGGATGGCAAACTGCATATATATTCTATAGCAGGTGATACACTTACGGAAGAGGCTGTACTAGAGAAACATCGGGGTGCTATCAGTGTCATACGTTACTCTCCAGATGTTTCAATGATTGCATCAGGAGATTTGAACCGAGAAGCTGTCATCTGGGATCGTGCCTCCCGAGAGGTCAAGCTTAAGAATATGCTGTACCATACTGCTCGAATAAACTGCCTTGCTTGGTCTCCCGATAACAGCATGGTTGCTACTGGATCACTTGACACATGTGTCATTATATATGAAGTTGACAAGCCTGCATCCAGCCGTGTAACCATCAAGGGTGCTCATTTGGGCGGGGTGTATGGGATAGCTTTCACCGATGAGCACACAGTCGTAAGCTCTGGCGAGGATGCCTTTGTTCGTGTGTGGAGGTTGACTCCACAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005856 GO:0005884 GO:0005938 GO:0006996 GO:0007010 GO:0007015 GO:0008064 GO:0008092 GO:0008150 GO:0008154 GO:0009987 GO:0010638 GO:0015629 GO:0016043 GO:0022411 GO:0030029 GO:0030036 GO:0030042 GO:0030832 GO:0030834 GO:0030836 GO:0030863 GO:0030864 GO:0031461 GO:0032535 GO:0032956 GO:0032970 GO:0032984 GO:0032991 GO:0033043 GO:0042641 GO:0042643 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043243 GO:0043244 GO:0043624 GO:0043933 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0044877 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051015 GO:0051128 GO:0051130 GO:0051261 GO:0051493 GO:0051495 GO:0065007 GO:0065008 GO:0071840 GO:0071944 GO:0080008 GO:0090066 GO:0097435 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:0110053 GO:1901879 GO:1901881 GO:1902494 GO:1902903 GO:1902905 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

609

Amino Acids

65.87

Weight (kDa)

5.83

Isoelectric Point (pI)

28.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_CDC20-Fz	PF24807	30 - 202	1.1e-07	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	37 - 211	8.7e-13	WDR3 first beta-propeller domain
Beta-prop_EML	PF23409	53 - 261	7.7e-15	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR5	PF25175	55 - 273	1.2e-29	WDR5 beta-propeller domain
Beta-prop_WDR3_2nd	PF25172	66 - 353	1.8e-11	WDR3 second beta-propeller domain
Beta-prop_THOC3	PF25174	102 - 174	6.6e-06	THOC3 beta-propeller domain
WD40_Gbeta	PF25391	102 - 265	5.9e-10	G protein beta WD-40 repeat protein
WD40_WDHD1_1st	PF24817	105 - 262	3.9e-09	WDHD1 first WD40 domain
WD40_MABP1-WDR62_2nd	PF24782	106 - 352	1.2e-08	MABP1/WDR62 second WD40 domain
Beta-prop_WDR36-Utp21_2nd	PF25168	113 - 263	1e-06	WDR36/Utp21 second beta-propeller domain
WD40_Prp19	PF24814	129 - 264	1.4e-14	Prp19 WD40 domain
Beta-prop_EML_2	PF23414	148 - 262	8.5e-08	Echinoderm microtubule-associated protein second beta-propeller
WDR55	PF24796	148 - 391	1.1e-12	WDR55
Beta-prop_WDR3_1st	PF25173	184 - 262	1.6e-09	WDR3 first beta-propeller domain
WD40_CDC20-Fz	PF24807	184 - 263	9.4e-06	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	187 - 264	6.9e-13	THOC3 beta-propeller domain
EIF3I	PF24805	187 - 264	1.9e-08	EIF3I
Beta-prop_CAF1B_HIR1	PF24105	187 - 264	1.5e-06	CAF1B/HIR1 beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	218 - 378	9.7e-09	TEP-1 second beta-propeller
WD40	PF00400	230 - 261	9.1e-09	WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_1st	PF25171	297 - 561	2.1e-08	WDR36/Utp21 first beta-propeller
WD40_Prp19	PF24814	326 - 519	1.7e-06	Prp19 WD40 domain
WD40_WDHD1_1st	PF24817	372 - 525	1.6e-06	WDHD1 first WD40 domain
Beta-prop_WDR3_2nd	PF25172	436 - 564	9.6e-08	WDR3 second beta-propeller domain
Beta-prop_EML_2	PF23414	437 - 543	6e-09	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st	PF25173	442 - 524	4.3e-09	WDR3 first beta-propeller domain
WD40_Gbeta	PF25391	449 - 604	2.9e-07	G protein beta WD-40 repeat protein
Beta-prop_WDR5	PF25175	450 - 605	9.2e-21	WDR5 beta-propeller domain
WD40_CDC20-Fz	PF24807	450 - 547	1.2e-09	CDC20/Fizzy WD40 domain
Beta-prop_WDR19_1st	PF23389	462 - 604	3.9e-06	WDR19 first beta-propeller
Beta-prop_WDR36-Utp21_1st	PF25171	463 - 604	2.8e-08	WDR36/Utp21 first beta-propeller
Beta-prop_CAF1B_HIR1	PF24105	465 - 605	7.3e-10	CAF1B/HIR1 beta-propeller domain
Beta-prop_IFT140_1st	PF23383	467 - 565	8.8e-06	IFT140 first beta-propeller
WD40_CDC20-Fz	PF24807	488 - 606	2.1e-13	CDC20/Fizzy WD40 domain
WDR55	PF24796	494 - 604	2.6e-07	WDR55
Beta-prop_EML_2	PF23414	526 - 599	4.2e-06	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_TEP1_2nd	PF25047	529 - 606	1.1e-07	TEP-1 second beta-propeller
WD40_WDHD1_1st	PF24817	533 - 608	1.8e-06	WDHD1 first WD40 domain
Beta-prop_THOC3	PF25174	533 - 606	1.4e-10	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	533 - 608	1.4e-10	WDR3 first beta-propeller domain
WD40	PF00400	533 - 561	1.5e-06	WD domain, G-beta repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013291)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G01330 AT3G18060
fragaria_vesca	FvH4_5g17110
malus_domestica	MD06G1229500.v1.1 MD14G1234400.v1.1
prunus_persica	Prupe.5G230600_v2.0.a1 Prupe.5G230600_v2.0.a1 Prupe.5G230600_v2.0.a1
pyrus_communis	pycom06g20050 pycom14g19520
rosa_chinensis	RchiOBHm_Chr7g0178701
rosa_laevigata	RLG00000005421
rosa_multiflora	Rmu_sc0002320.1_g000019
rosa_roxburghii	Rroxscaffold_3G00274660
rosa_rugosa	Rorug06G0418700
rosa_samantha	Rh7AG018100 Rh7BG017600
rosa_wichuraiana	Rw7G001460

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1421
AasI	GACNNNNNNGTC	2 cut(s)	152, 1670
AatII	GACGTC	2 cut(s)	150, 231
Acc36I	ACCTGC	1 cut(s)	1421
AccB1I	GGYRCC	1 cut(s)	236
AccI	GTMKAC	4 cut(s)	38, 156, 407, 1025
AccII	CGCG	4 cut(s)	48, 161, 218, 384
AcoI	YGGCCR	3 cut(s)	49, 177, 691
AcsI	RAATTY	2 cut(s)	536, 565
AcuI	CTGAAG	1 cut(s)	1238
AcyI	GRCGYC	2 cut(s)	147, 228
AfaI	GTAC	3 cut(s)	587, 1458, 1595
AfiI	CCNNNNNNNGG	3 cut(s)	393, 836, 1184
AflII	CTTAAG	1 cut(s)	1580
AflIII	ACRYGT	2 cut(s)	824, 1667
AgsI	TTSAA	9 cut(s)	289, 460, 535, 541, 1061, 1130, 1281, 1512, 1534
AjnI	CCWGG	2 cut(s)	504, 829
AjuI	GAANNNNNNNTTGG	2 cut(s)	1374, 1406
Alw21I	GWGCWC	3 cut(s)	168, 1729, 1770
Alw26I	GTCTC	5 cut(s)	235, 552, 1091, 1263, 1632
AlwNI	CAGNNNCTG	5 cut(s)	249, 745, 1211, 1295, 1358
Ama87I	CYCGRG	2 cut(s)	298, 1566
AoxI	GGCC	6 cut(s)	18, 49, 177, 524, 691, 865
ApeKI	GCWGC	3 cut(s)	702, 1121, 1311
ApoI	RAATTY	2 cut(s)	536, 565
Asp700I	GAANNNNTTC	1 cut(s)	284
AspLEI	GCGC	2 cut(s)	48, 386
AspS9I	GGNCC	4 cut(s)	255, 292, 324, 524
AsuC2I	CCSGG	2 cut(s)	53, 176
AsuHPI	GGTGA	5 cut(s)	506, 820, 1199, 1445, 1750
AsuII	TTCGAA	1 cut(s)	434
AvaI	CYCGRG	2 cut(s)	298, 1566
AvaII	GGWCC	3 cut(s)	255, 292, 324
BalI	TGGCCA	1 cut(s)	693
BamHI	GGATCC	1 cut(s)	57
BanI	GGYRCC	1 cut(s)	236
BarI	GAAGNNNNNNTAC	2 cut(s)	1440, 1472
BbsI	GAAGAC	1 cut(s)	659
Bbv12I	GWGCWC	3 cut(s)	168, 1729, 1770
BbvI	GCAGC	3 cut(s)	714, 1133, 1323
BceAI	ACGGC	4 cut(s)	19, 137, 220, 1692
BcgI	CGANNNNNNTGC	2 cut(s)	1596, 1630
BciT130I	CCWGG	2 cut(s)	506, 831
BclI	TGATCA	4 cut(s)	117, 880, 912, 1189
BcnI	CCSGG	2 cut(s)	53, 176
BcoDI	GTCTC	5 cut(s)	235, 552, 1091, 1263, 1632
BfaI	CTAG	1 cut(s)	1460
BfmI	CTRYAG	1 cut(s)	1425
BfrI	CTTAAG	1 cut(s)	1580
BfuAI	ACCTGC	1 cut(s)	1421
BglI	GCCNNNNNGGC	2 cut(s)	176, 699
BisI	GCNGC	6 cut(s)	49, 355, 703, 865, 1122, 1312
BlsI	GCNGC	6 cut(s)	50, 356, 704, 866, 1123, 1313
Bme1390I	CCNGG	4 cut(s)	53, 176, 506, 831
Bme18I	GGWCC	3 cut(s)	255, 292, 324
BmeT110I	CYCGRG	2 cut(s)	298, 1566
BmgT120I	GGNCC	4 cut(s)	255, 292, 324, 524
BmiI	GGNNCC	5 cut(s)	59, 92, 238, 256, 257
BmrFI	CCNGG	4 cut(s)	53, 176, 506, 831
BmsI	GCATC	5 cut(s)	1192, 1364, 1529, 1709, 1783
BpiI	GAAGAC	1 cut(s)	659
BplI	GAGNNNNNCTC	1 cut(s)	1804
BpmI	CTGGAG	4 cut(s)	302, 576, 742, 1485
Bpu14I	TTCGAA	1 cut(s)	434
BpuEI	CTTGAG	1 cut(s)	260
BpuMI	CCSGG	2 cut(s)	53, 176
BsaBI	GATNNNNATC	1 cut(s)	62
BsaHI	GRCGYC	2 cut(s)	147, 228
BsaI	GGTCTC	1 cut(s)	1632
BsaJI	CCNNGG	3 cut(s)	15, 52, 1336
Bsc4I	CCNNNNNNNGG	3 cut(s)	393, 836, 1184
Bse118I	RCCGGY	1 cut(s)	87
Bse1I	ACTGG	2 cut(s)	319, 1657
Bse8I	GATNNNNATC	1 cut(s)	62
BseBI	CCWGG	2 cut(s)	506, 831
BseDI	CCNNGG	3 cut(s)	15, 52, 1336
BseGI	GGATG	5 cut(s)	799, 853, 1408, 1700, 1798
BseJI	GATNNNNATC	1 cut(s)	62
BseLI	CCNNNNNNNGG	3 cut(s)	393, 836, 1184
BseMII	CTCAG	2 cut(s)	636, 939
BseNI	ACTGG	2 cut(s)	319, 1657
BseRI	GAGGAG	1 cut(s)	83
BseX3I	CGGCCG	1 cut(s)	49
BseXI	GCAGC	3 cut(s)	714, 1133, 1323
Bsh1236I	CGCG	4 cut(s)	48, 161, 218, 384
Bsh1285I	CGRYCG	1 cut(s)	52
BshFI	GGCC	6 cut(s)	20, 51, 179, 526, 693, 867
BshNI	GGYRCC	1 cut(s)	236
BsiEI	CGRYCG	1 cut(s)	52
BsiHKAI	GWGCWC	3 cut(s)	168, 1729, 1770
BsiHKCI	CYCGRG	2 cut(s)	298, 1566
BsiSI	CCGG	5 cut(s)	52, 69, 88, 176, 234
BslFI	GGGAC	1 cut(s)	268
BslI	CCNNNNNNNGG	3 cut(s)	393, 836, 1184
BsmAI	GTCTC	5 cut(s)	235, 552, 1091, 1263, 1632
BsmBI	CGTCTC	1 cut(s)	235
BsmFI	GGGAC	1 cut(s)	268
BsmI	GAATGC	1 cut(s)	430
BsnI	GGCC	6 cut(s)	20, 51, 179, 526, 693, 867
Bso31I	GGTCTC	1 cut(s)	1632
BsoBI	CYCGRG	2 cut(s)	298, 1566
Bsp119I	TTCGAA	1 cut(s)	434
Bsp1286I	GDGCHC	3 cut(s)	168, 1729, 1770
BspANI	GGCC	6 cut(s)	20, 51, 179, 526, 693, 867
BspCNI	CTCAG	2 cut(s)	635, 938
BspFNI	CGCG	4 cut(s)	48, 161, 218, 384
BspHI	TCATGA	1 cut(s)	123
BspLI	GGNNCC	5 cut(s)	59, 92, 238, 256, 257
BspMI	ACCTGC	1 cut(s)	1421
BspT104I	TTCGAA	1 cut(s)	434
BspT107I	GGYRCC	1 cut(s)	236
BspTI	CTTAAG	1 cut(s)	1580
BspTNI	GGTCTC	1 cut(s)	1632
BsrFI	RCCGGY	1 cut(s)	87
BsrI	ACTGG	2 cut(s)	319, 1657
BssAI	RCCGGY	1 cut(s)	87
BssECI	CCNNGG	3 cut(s)	15, 52, 1336
BssNI	GRCGYC	2 cut(s)	147, 228
BssT1I	CCWWGG	1 cut(s)	1336
Bst2UI	CCWGG	2 cut(s)	506, 831
Bst6I	CTCTTC	1 cut(s)	1443
BstACI	GRCGYC	2 cut(s)	147, 228
BstAFI	CTTAAG	1 cut(s)	1580
BstBI	TTCGAA	1 cut(s)	434
BstC8I	GCNNGC	5 cut(s)	28, 32, 168, 352, 1698
BstDEI	CTNAG	3 cut(s)	622, 716, 925
BstENI	CCTNNNNNAGG	1 cut(s)	834
BstF5I	GGATG	5 cut(s)	799, 853, 1408, 1700, 1798
BstFNI	CGCG	4 cut(s)	48, 161, 218, 384
BstHHI	GCGC	2 cut(s)	48, 386
BstMAI	GTCTC	5 cut(s)	235, 552, 1091, 1263, 1632
BstMCI	CGRYCG	1 cut(s)	52
BstMWI	GCNNNNNNNGC	6 cut(s)	158, 167, 176, 185, 360, 699
BstNI	CCWGG	2 cut(s)	506, 831
BstNSI	RCATGY	4 cut(s)	492, 828, 856, 1671
BstSCI	CCNGG	4 cut(s)	51, 174, 504, 829
BstSFI	CTRYAG	1 cut(s)	1425
BstUI	CGCG	4 cut(s)	48, 161, 218, 384
BstV1I	GCAGC	3 cut(s)	714, 1133, 1323
BstV2I	GAAGAC	1 cut(s)	659
BstX2I	RGATCY	3 cut(s)	57, 110, 246
BstYI	RGATCY	3 cut(s)	57, 110, 246
BstZI	CGGCCG	1 cut(s)	49
BsuRI	GGCC	6 cut(s)	20, 51, 179, 526, 693, 867
BtsCI	GGATG	5 cut(s)	799, 853, 1408, 1700, 1798
BtsIMutI	CAGTG	3 cut(s)	326, 612, 1489
BveI	ACCTGC	1 cut(s)	1421
Cac8I	GCNNGC	5 cut(s)	28, 32, 168, 352, 1698
CaiI	CAGNNNCTG	5 cut(s)	249, 745, 1211, 1295, 1358
CciI	TCATGA	1 cut(s)	123
CfoI	GCGC	2 cut(s)	48, 386
Cfr10I	RCCGGY	1 cut(s)	87
Cfr13I	GGNCC	4 cut(s)	255, 292, 324, 524
CseI	GACGC	1 cut(s)	207
Csp6I	GTAC	3 cut(s)	586, 1457, 1594
CviQI	GTAC	3 cut(s)	586, 1457, 1594
DdeI	CTNAG	3 cut(s)	622, 716, 925
DrdI	GACNNNNNNGTC	2 cut(s)	152, 1670
DseDI	GACNNNNNNGTC	2 cut(s)	152, 1670
EaeI	YGGCCR	3 cut(s)	49, 177, 691
EagI	CGGCCG	1 cut(s)	49
Eam1104I	CTCTTC	1 cut(s)	1443
EarI	CTCTTC	1 cut(s)	1443
EciI	GGCGGA	2 cut(s)	211, 316
EclXI	CGGCCG	1 cut(s)	49
Eco130I	CCWWGG	1 cut(s)	1336
Eco147I	AGGCCT	1 cut(s)	20
Eco31I	GGTCTC	1 cut(s)	1632
Eco47I	GGWCC	3 cut(s)	255, 292, 324
Eco52I	CGGCCG	1 cut(s)	49
Eco57I	CTGAAG	1 cut(s)	1238
Eco88I	CYCGRG	2 cut(s)	298, 1566
EcoNI	CCTNNNNNAGG	1 cut(s)	834
EcoO109I	RGGNCCY	3 cut(s)	255, 292, 524
EcoRII	CCWGG	2 cut(s)	504, 829
EcoT14I	CCWWGG	1 cut(s)	1336
ErhI	CCWWGG	1 cut(s)	1336
Esp3I	CGTCTC	1 cut(s)	235
FaqI	GGGAC	1 cut(s)	268
FauI	CCCGC	1 cut(s)	1730
FbaI	TGATCA	4 cut(s)	117, 880, 912, 1189
FblI	GTMKAC	4 cut(s)	38, 156, 407, 1025
Fnu4HI	GCNGC	6 cut(s)	49, 355, 703, 865, 1122, 1312
FokI	GGATG	5 cut(s)	806, 860, 1415, 1687, 1805
Fsp4HI	GCNGC	6 cut(s)	49, 355, 703, 865, 1122, 1312
FspBI	CTAG	1 cut(s)	1460
GlaI	GCGC	2 cut(s)	47, 385
GluI	GCNGC	6 cut(s)	49, 355, 703, 865, 1122, 1312
GsuI	CTGGAG	4 cut(s)	302, 576, 742, 1485
HaeIII	GGCC	6 cut(s)	20, 51, 179, 526, 693, 867
HapII	CCGG	5 cut(s)	52, 69, 88, 176, 234
HgaI	GACGC	1 cut(s)	207
HhaI	GCGC	2 cut(s)	48, 386
Hin1I	GRCGYC	2 cut(s)	147, 228
Hin6I	GCGC	2 cut(s)	46, 384
HinP1I	GCGC	2 cut(s)	46, 384
HincII	GTYRAC	5 cut(s)	39, 408, 1326, 1690, 1818
HindII	GTYRAC	5 cut(s)	39, 408, 1326, 1690, 1818
HindIII	AAGCTT	1 cut(s)	1577
HinfI	GANTC	7 cut(s)	192, 398, 724, 742, 1208, 1286, 1819
HpaII	CCGG	5 cut(s)	52, 69, 88, 176, 234
HphI	GGTGA	5 cut(s)	506, 820, 1199, 1445, 1750
Hpy166II	GTNNAC	9 cut(s)	39, 157, 408, 574, 1026, 1326, 1344, 1690, 1818
Hpy188I	TCNGA	5 cut(s)	306, 625, 671, 685, 747
Hpy8I	GTNNAC	9 cut(s)	39, 157, 408, 574, 1026, 1326, 1344, 1690, 1818
Hpy99I	CGWCG	9 cut(s)	40, 43, 149, 152, 188, 230, 314, 335, 350
HpyAV	CCTTC	8 cut(s)	353, 397, 515, 625, 799, 1087, 1228, 1262
HpyCH4IV	ACGT	3 cut(s)	147, 228, 1492
HpyCH4V	TGCA	6 cut(s)	1121, 1205, 1367, 1414, 1520, 1700
HpyF10VI	GCNNNNNNNGC	6 cut(s)	158, 167, 176, 185, 360, 699
HpyF3I	CTNAG	3 cut(s)	622, 716, 925
HpySE526I	ACGT	3 cut(s)	147, 228, 1492
Hsp92I	GRCGYC	2 cut(s)	147, 228
HspAI	GCGC	2 cut(s)	46, 384
KflI	GGGWCCC	1 cut(s)	255
Ksp22I	TGATCA	4 cut(s)	117, 880, 912, 1189
LmnI	GCTCC	4 cut(s)	43, 96, 163, 1292
Lsp1109I	GCAGC	3 cut(s)	714, 1133, 1323
LweI	GCATC	5 cut(s)	1192, 1364, 1529, 1709, 1783
MaeI	CTAG	1 cut(s)	1460
MaeII	ACGT	3 cut(s)	147, 228, 1492
MaeIII	GTNAC	6 cut(s)	484, 889, 932, 1348, 1493, 1711
MboII	GAAGA	8 cut(s)	664, 676, 698, 985, 1137, 1142, 1145, 1460
MfeI	CAATTG	1 cut(s)	1281
MflI	RGATCY	3 cut(s)	57, 110, 246
MhlI	GDGCHC	3 cut(s)	168, 1729, 1770
MlsI	TGGCCA	1 cut(s)	693
MluCI	AATT	7 cut(s)	511, 536, 565, 1044, 1082, 1137, 1281
MluNI	TGGCCA	1 cut(s)	693
MlyI	GAGTC	2 cut(s)	733, 1813
MmeI	TCCRAC	3 cut(s)	108, 284, 1048
Mox20I	TGGCCA	1 cut(s)	693
MroXI	GAANNNNTTC	1 cut(s)	284
MscI	TGGCCA	1 cut(s)	693
MseI	TTAA	3 cut(s)	1050, 1332, 1581
MslI	CAYNNNNRTG	2 cut(s)	823, 1473
Msp20I	TGGCCA	1 cut(s)	693
MspA1I	CMGCKG	2 cut(s)	758, 1124
MspCI	CTTAAG	1 cut(s)	1580
MspI	CCGG	5 cut(s)	52, 69, 88, 176, 234
MspR9I	CCNGG	4 cut(s)	53, 176, 506, 831
MunI	CAATTG	1 cut(s)	1281
Mva1269I	GAATGC	1 cut(s)	430
MvaI	CCWGG	2 cut(s)	506, 831
MvnI	CGCG	4 cut(s)	48, 161, 218, 384
MwoI	GCNNNNNNNGC	6 cut(s)	158, 167, 176, 185, 360, 699
NciI	CCSGG	2 cut(s)	53, 176
NlaIV	GGNNCC	5 cut(s)	59, 92, 238, 256, 257
NmeAIII	GCCGAG	1 cut(s)	40
NmuCI	GTSAC	3 cut(s)	484, 932, 1348
NspI	RCATGY	4 cut(s)	492, 828, 856, 1671
NspV	TTCGAA	1 cut(s)	434
PagI	TCATGA	1 cut(s)	123
PaqCI	CACCTGC	1 cut(s)	1421
PceI	AGGCCT	1 cut(s)	20
PciI	ACATGT	2 cut(s)	824, 1667
PcsI	WCGNNNNNNNCGW	4 cut(s)	150, 222, 354, 1564
PctI	GAATGC	1 cut(s)	430
PdmI	GAANNNNTTC	1 cut(s)	284
PfeI	GAWTC	5 cut(s)	192, 398, 742, 1208, 1286
PflFI	GACNNNGTC	1 cut(s)	751
PfoI	TCCNGGA	1 cut(s)	829
PkrI	GCNGC	6 cut(s)	50, 356, 704, 866, 1123, 1313
PleI	GAGTC	2 cut(s)	732, 1813
PpsI	GAGTC	2 cut(s)	732, 1813
PpuMI	RGGWCCY	2 cut(s)	255, 292
PscI	ACATGT	2 cut(s)	824, 1667
Psp5II	RGGWCCY	2 cut(s)	255, 292
Psp6I	CCWGG	2 cut(s)	504, 829
PspGI	CCWGG	2 cut(s)	504, 829
PspN4I	GGNNCC	5 cut(s)	59, 92, 238, 256, 257
PspPI	GGNCC	4 cut(s)	255, 292, 324, 524
PspPPI	RGGWCCY	2 cut(s)	255, 292
PstNI	CAGNNNCTG	5 cut(s)	249, 745, 1211, 1295, 1358
PsuI	RGATCY	3 cut(s)	57, 110, 246
PsyI	GACNNNGTC	1 cut(s)	751
PvuII	CAGCTG	1 cut(s)	1124
RsaI	GTAC	3 cut(s)	587, 1458, 1595
RsaNI	GTAC	3 cut(s)	586, 1457, 1594
RseI	CAYNNNNRTG	2 cut(s)	823, 1473
SalI	GTCGAC	2 cut(s)	37, 406
SaqAI	TTAA	3 cut(s)	1050, 1332, 1581
SatI	GCNGC	6 cut(s)	49, 355, 703, 865, 1122, 1312
Sau96I	GGNCC	4 cut(s)	255, 292, 324, 524
SchI	GAGTC	2 cut(s)	733, 1813
ScrFI	CCNGG	4 cut(s)	53, 176, 506, 831
SduI	GDGCHC	3 cut(s)	168, 1729, 1770
SfaNI	GCATC	5 cut(s)	1192, 1364, 1529, 1709, 1783
SfcI	CTRYAG	1 cut(s)	1425
SfuI	TTCGAA	1 cut(s)	434
SgrDI	CGTCGACG	1 cut(s)	37
SinI	GGWCC	3 cut(s)	255, 292, 324
SmiMI	CAYNNNNRTG	2 cut(s)	823, 1473
SmlI	CTYRAG	2 cut(s)	275, 1580
SmoI	CTYRAG	2 cut(s)	275, 1580
Sse9I	AATT	7 cut(s)	511, 536, 565, 1044, 1082, 1137, 1281
SseBI	AGGCCT	1 cut(s)	20
SspI	AATATT	1 cut(s)	979
SspMI	CTAG	1 cut(s)	1460
StuI	AGGCCT	1 cut(s)	20
StyD4I	CCNGG	4 cut(s)	51, 174, 504, 829
StyI	CCWWGG	1 cut(s)	1336
TaiI	ACGT	3 cut(s)	150, 231, 1495
TaqI	TCGA	9 cut(s)	38, 132, 144, 309, 407, 423, 434, 563, 1606
TasI	AATT	7 cut(s)	511, 536, 565, 1044, 1082, 1137, 1281
TatI	WGTACW	1 cut(s)	1456
TauI	GCSGC	3 cut(s)	51, 357, 867
TfiI	GAWTC	5 cut(s)	192, 398, 742, 1208, 1286
Tru1I	TTAA	3 cut(s)	1050, 1332, 1581
Tru9I	TTAA	3 cut(s)	1050, 1332, 1581
TscAI	CASTG	3 cut(s)	326, 619, 1489
TseFI	GTSAC	3 cut(s)	484, 932, 1348
TseI	GCWGC	3 cut(s)	702, 1121, 1311
Tsp45I	GTSAC	3 cut(s)	484, 932, 1348
TspDTI	ATGAA	7 cut(s)	140, 379, 534, 669, 975, 986, 1698
TspGWI	ACGGA	4 cut(s)	56, 103, 348, 1460
TspRI	CASTG	3 cut(s)	326, 619, 1489
Tth111I	GACNNNGTC	1 cut(s)	751
Vha464I	CTTAAG	1 cut(s)	1580
VpaK11BI	GGWCC	3 cut(s)	255, 292, 324
XagI	CCTNNNNNAGG	1 cut(s)	834
XapI	RAATTY	2 cut(s)	536, 565
XceI	RCATGY	4 cut(s)	492, 828, 856, 1671
XmiI	GTMKAC	4 cut(s)	38, 156, 407, 1025
XmnI	GAANNNNTTC	1 cut(s)	284
XspI	CTAG	1 cut(s)	1460
ZraI	GACGTC	2 cut(s)	148, 229

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.