MD06G1232200.v1.1

NF-X1-type zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0012624
Physical Location & Seq
Forward (+)
36312099 .. 36318005
5907 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1232200.v1.1.491

Sequence Viewer

Length: 2277 bp
ATGAGTACTGCTCCCTTATACGTCTTCGGGACATTGACTGCAGATGATATCACATCAGAGGAGACCGTTTATGAACTATTCTCCTTCATTTTCTGGACTATTAACATCATTTCTTTACTCAAGTATGCATTTATAGTATTAACAGCTGATGACAATGGAGAAGGTGGTACTTTTGCTTTGTATTCACTTCTGTGTAGGCATGCCAAAGTTGGTTTGCTTCCAAATGAAAGTAGTGCTAATGACGTTATGCATTATGAGACGGGAAGTCCTTTCAAGATAAAGGCAGAATCAAGGGCTCGAAGAGCCATAGAAAAGCTTAGATGTGGCCATTATTTGATGCTGTTCCTGGCTCTTTTCGGTTCCTGCATGACAATTGGCATTGGGGTGCTTACCCCAACTCTTTCTGTCTATTCAGTTTCATCTGGAATTCAAAGATCATTGTCGGATATTTCACACAGAATTTCAGCGTCGGAAAAACAAAGGGAGGCCATATCTAGAGCTTTAGAGAAATATGTCCCGGTTTCCACTGCATCTGCTATATTGGTGTGCCTCTTCACACTACAGCACTACGGGACTCGTAAAATTGGGTTTATTTTTGCTCCAATTATCGTCGTTTGGCTGTTCTTTATTGGTGGAGGGGGTGTATATAACATTTTCCACTGGAACAAAGAAATTCTCCTTGCTACCTCCCCCATGTACATGTACAGATTTTTCAGGAATATTCACAGCAAAAGTTGGAGGTCATTAGGCAGCATTGCTTTATGTGTAGCAGGATCGGAAGCAATGTTTACATCTCTAGGTCATTTCAGAAGGAAATCAATCAAGATGACATTTGTATGCTTCATATATCCAGTCCTTGTTTTAAGCTACGCCGGTCAGGCTGCATATATCTCCAAAAACTTGGATGTTAGAGATTTCAATCATCTCAGTCAATCCATACCTAAACAAATGCGCCACTGGTTTGTAGCATTTTCCCTTCTTGCTTCTGTTGTGGGAAGCCAGGCAACCATAACAGCATGTTTCTCCATTATAAATCAGTGCCTGGCACTTGGTTGTTTTCCGAGAGTAAAAGTTATTCATACATCAGATAAGATACACGGGCAGGTCTACATTCCTGATATCAACTGGCTATTGATGGTTCTAAGCCTCGTTGTTACGATTGGTTTCCATGATATTATCAAAATTGGTAGCGCGACAGGTTTGGCTGTAAGTTCTGGAATGCTTGTAACGACTTGTCTCATGTCACTAGTAATTGCGCTATATTGGGAGAAAAGTTTGTTTGAGTCGGTCTGCTGTCTAATATTCTTTGGATCCATCGAGGTGATGTATGTATCGGCTTGTATGTTGAATTTTCACAAGGGAGCTTGGTATTTGGTTGTCCTTTTGGCAATGTCCCTGACTATCATGCTTTCATGGCATTACGGAACCAAGAAGAAGCTCGAATTTGATATACAAAACAAGGTCTCTGCAGAATGGCTTATAGGCATTAGCCCGGGCCTCGGAGTTACTAGGGTGCCCGGGATTGGTTTCATTTATTCTGATATAGTGACAGGAATTCCAGCTTTCTTCTCACACTTCATTACCAATCTTCCAGCGTTTCACCAAGTTTTGATTTTCGTGTCCTTCAAGTCTCTGCCAATGCCTTATGTCCCTCCAAGTCGGAGGTATCTTGTTGGCAGGGTTGGCCCGAGGAATTTTAAGATATATCGCTGTGTTGTGAGATATGGATATTGTGATCCTATTAGGGACACTGATAATTTTGAAGAACAGATTATTAGTTCGATTGGTGAATTCATTTGCATGGAAGAAAATGATTTTGATTTCCAAAATTCATCCGAAGGAAGAATGGTGGTTATTGGAAACCCACCAGCTGATGGAACCGGAAGTGCTTTGATTCCCTTGAATGATACAAATTCATATGAGATGGAGTGCGTAAGTATGGCGAGCAACGAAACTCGGTCTCCATTAGGTGATGCAATCGAGAGTGGTTTTACGCCGGTGATGAGAAAAAAAGTCCGGTTCATGTTGCCTCCGAATAGTCCGCAAATGCACGTATCTGTGAGGGAGGAGCTGCAAGAACTGATTGATGCTAGGGAGAGTGGGACTGCATACTTCTTAGGGCAGGTGCATTTAGCAGTGCGTGACGGATCAGACATTCTAAAGCGATTGCTGATCATGACTTATGTGTTTCTTGATAAAAATTGCAGAGAGCCTCCAGTTGCACTCAATATTCCTCATGCTGCTCTTGTTGAGGTTGGCATGGTCTGTTGCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000122 GO:0000976 GO:0000977 GO:0000981 GO:0000982 GO:0001012 GO:0001067 GO:0001078 GO:0001227 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006082 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009636 GO:0009642 GO:0009651 GO:0009696 GO:0009697 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010188 GO:0010310 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010817 GO:0016053 GO:0016070 GO:0016999 GO:0017000 GO:0017144 GO:0018130 GO:0018958 GO:0019219 GO:0019222 GO:0019438 GO:0019752 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032774 GO:0032787 GO:0034641 GO:0034645 GO:0034654 GO:0042221 GO:0042445 GO:0042446 GO:0042537 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046189 GO:0046394 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051193 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0065008 GO:0071704 GO:0072330 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0098542 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901615 GO:1901617 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000112 GO:2000113 GO:2000377 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko05165 map05165
Pfam Domains
Protein Families
K_trans R3H zf-NF-X1

Protein Analysis

759

Amino Acids

84.6

Weight (kDa)

8.26

Isoelectric Point (pI)

43.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
K_trans PF02705 2 - 493 8.5e-121 K+ potassium transporter integral membrane domain
K_trans_C PF22776 510 - 756 3.4e-24 K+ potassium transporter C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1031
AarI CACCTGC 1 cut(s) 2113
Acc36I ACCTGC 2 cut(s) 1093, 2113
AccB1I GGYRCC 1 cut(s) 1513
AccB7I CCANNNNNTGG 1 cut(s) 1874
AccI GTMKAC 1 cut(s) 1107
AccII CGCG 1 cut(s) 1193
AciI CCGC 1 cut(s) 2042
AclWI GGATC 5 cut(s) 781, 1305, 1318, 1730, 2155
AcoI YGGCCR 1 cut(s) 325
AfaI GTAC 4 cut(s) 7, 169, 698, 704
AfiI CCNNNNNNNGG 3 cut(s) 1499, 1523, 1874
AflIII ACRYGT 1 cut(s) 699
AgsI TTSAA 7 cut(s) 274, 431, 919, 1348, 1627, 1763, 1903
AhdI GACNNNNNGTC 1 cut(s) 264
AhlI ACTAGT 1 cut(s) 1246
AjnI CCWGG 3 cut(s) 345, 999, 1041
AleI CACNNNNGTG 1 cut(s) 190
AluBI AGCT 9 cut(s) 146, 316, 500, 867, 1364, 1438, 1562, 1871, 2071
AluI AGCT 9 cut(s) 146, 316, 500, 867, 1364, 1438, 1562, 1871, 2071
Alw26I GTCTC 6 cut(s) 56, 251, 1241, 1468, 1635, 1965
AlwI GGATC 5 cut(s) 781, 1305, 1318, 1730, 2155
AlwNI CAGNNNCTG 1 cut(s) 1042
Ama87I CYCGRG 3 cut(s) 1492, 1517, 1687
AoxI GGCC 4 cut(s) 325, 486, 1495, 1684
ApeKI GCWGC 4 cut(s) 750, 881, 2071, 2240
AspLEI GCGC 3 cut(s) 954, 1193, 1258
AspS9I GGNCC 2 cut(s) 1495, 1685
AsuC2I CCSGG 5 cut(s) 518, 1493, 1494, 1518, 1519
AsuHPI GGTGA 5 cut(s) 1333, 1592, 1799, 1982, 2011
AvaI CYCGRG 3 cut(s) 1492, 1517, 1687
BaeGI GKGCMC 1 cut(s) 1518
BalI TGGCCA 1 cut(s) 327
BamHI GGATCC 1 cut(s) 1310
BanI GGYRCC 1 cut(s) 1513
BanII GRGCYC 1 cut(s) 298
BbsI GAAGAC 1 cut(s) 16
BbvI GCAGC 4 cut(s) 762, 868, 2058, 2227
BccI CCATC 4 cut(s) 1129, 1322, 1868, 1918
BciT130I CCWGG 3 cut(s) 347, 1001, 1043
BclI TGATCA 1 cut(s) 2172
BcnI CCSGG 5 cut(s) 518, 1493, 1494, 1518, 1519
BcoDI GTCTC 6 cut(s) 56, 251, 1241, 1468, 1635, 1965
BcuI ACTAGT 1 cut(s) 1246
BfaI CTAG 5 cut(s) 495, 797, 1247, 1509, 2091
BfmI CTRYAG 3 cut(s) 39, 560, 1467
BfuAI ACCTGC 2 cut(s) 1093, 2113
BglI GCCNNNNNGGC 1 cut(s) 878
BisI GCNGC 4 cut(s) 751, 882, 2072, 2241
BlsI GCNGC 4 cut(s) 752, 883, 2073, 2242
BmcAI AGTACT 1 cut(s) 7
Bme1390I CCNGG 8 cut(s) 347, 518, 1001, 1043, 1493, 1494, 1518, 1519
BmeRI GACNNNNNGTC 1 cut(s) 264
BmeT110I CYCGRG 3 cut(s) 1492, 1517, 1687
BmgT120I GGNCC 2 cut(s) 1495, 1685
BmiI GGNNCC 5 cut(s) 361, 1312, 1426, 1515, 1879
BmrFI CCNGG 8 cut(s) 347, 518, 1001, 1043, 1493, 1494, 1518, 1519
BmsI GCATC 4 cut(s) 327, 539, 1963, 2077
BpiI GAAGAC 1 cut(s) 16
BplI GAGNNNNNCTC 1 cut(s) 27
BpmI CTGGAG 1 cut(s) 2199
BpuEI CTTGAG 1 cut(s) 104
BpuMI CCSGG 5 cut(s) 518, 1493, 1494, 1518, 1519
BsaAI YACGTR 1 cut(s) 2053
BsaBI GATNNNNATC 1 cut(s) 918
BsaI GGTCTC 3 cut(s) 56, 1468, 1965
BsaJI CCNNGG 4 cut(s) 1492, 1498, 1517, 1688
BsaWI WCCGGW 2 cut(s) 1880, 2016
BsaXI ACNNNNNCTCC 4 cut(s) 627, 657, 1945, 1975
Bsc4I CCNNNNNNNGG 3 cut(s) 1499, 1523, 1874
Bse118I RCCGGY 2 cut(s) 872, 1996
Bse1I ACTGG 5 cut(s) 665, 851, 962, 1130, 2216
Bse3DI GCAATG 3 cut(s) 753, 789, 1395
Bse8I GATNNNNATC 1 cut(s) 918
BseBI CCWGG 3 cut(s) 347, 1001, 1043
BseDI CCNNGG 4 cut(s) 1492, 1498, 1517, 1688
BseGI GGATG 2 cut(s) 910, 1832
BseJI GATNNNNATC 1 cut(s) 918
BseLI CCNNNNNNNGG 3 cut(s) 1499, 1523, 1874
BseMI GCAATG 3 cut(s) 753, 789, 1395
BseMII CTCAG 1 cut(s) 940
BseNI ACTGG 5 cut(s) 665, 851, 962, 1130, 2216
BseRI GAGGAG 2 cut(s) 74, 2081
BseSI GKGCMC 1 cut(s) 1518
BseXI GCAGC 4 cut(s) 762, 868, 2058, 2227
Bsh1236I CGCG 1 cut(s) 1193
BshFI GGCC 4 cut(s) 327, 488, 1497, 1686
BshNI GGYRCC 1 cut(s) 1513
BsiHKCI CYCGRG 3 cut(s) 1492, 1517, 1687
BsiSI CCGG 7 cut(s) 518, 873, 1493, 1518, 1881, 1997, 2017
BslFI GGGAC 7 cut(s) 43, 500, 586, 1378, 1634, 1760, 2116
BslI CCNNNNNNNGG 3 cut(s) 1499, 1523, 1874
BsmAI GTCTC 6 cut(s) 56, 251, 1241, 1468, 1635, 1965
BsmBI CGTCTC 1 cut(s) 251
BsmFI GGGAC 7 cut(s) 43, 500, 586, 1378, 1634, 1760, 2116
BsmI GAATGC 1 cut(s) 1224
BsnI GGCC 4 cut(s) 327, 488, 1497, 1686
Bso31I GGTCTC 3 cut(s) 56, 1468, 1965
BsoBI CYCGRG 3 cut(s) 1492, 1517, 1687
Bsp1286I GDGCHC 2 cut(s) 298, 1518
Bsp1407I TGTACA 2 cut(s) 696, 702
Bsp143I GATC 6 cut(s) 434, 773, 1310, 1735, 2147, 2172
BspACI CCGC 1 cut(s) 2042
BspANI GGCC 4 cut(s) 327, 488, 1497, 1686
BspCNI CTCAG 1 cut(s) 939
BspFNI CGCG 1 cut(s) 1193
BspHI TCATGA 1 cut(s) 2175
BspLI GGNNCC 5 cut(s) 361, 1312, 1426, 1515, 1879
BspMAI CTGCAG 2 cut(s) 43, 1471
BspMI ACCTGC 2 cut(s) 1093, 2113
BspPI GGATC 5 cut(s) 781, 1305, 1318, 1730, 2155
BspQI GCTCTTC 1 cut(s) 295
BspT107I GGYRCC 1 cut(s) 1513
BspTNI GGTCTC 3 cut(s) 56, 1468, 1965
BsrDI GCAATG 3 cut(s) 753, 789, 1395
BsrFI RCCGGY 2 cut(s) 872, 1996
BsrGI TGTACA 2 cut(s) 696, 702
BsrI ACTGG 5 cut(s) 665, 851, 962, 1130, 2216
BssAI RCCGGY 2 cut(s) 872, 1996
BssECI CCNNGG 4 cut(s) 1492, 1498, 1517, 1688
BssMI GATC 6 cut(s) 434, 773, 1310, 1735, 2147, 2172
Bst2UI CCWGG 3 cut(s) 347, 1001, 1043
Bst4CI ACNGT 1 cut(s) 67
Bst6I CTCTTC 2 cut(s) 295, 557
BstAUI TGTACA 2 cut(s) 696, 702
BstBAI YACGTR 1 cut(s) 2053
BstC8I GCNNGC 2 cut(s) 201, 1945
BstDEI CTNAG 4 cut(s) 317, 926, 1142, 2116
BstF5I GGATG 2 cut(s) 910, 1832
BstFNI CGCG 1 cut(s) 1193
BstHHI GCGC 3 cut(s) 954, 1193, 1258
BstKTI GATC 6 cut(s) 437, 776, 1313, 1738, 2150, 2175
BstMAI GTCTC 6 cut(s) 56, 251, 1241, 1468, 1635, 1965
BstMBI GATC 6 cut(s) 434, 773, 1310, 1735, 2147, 2172
BstMWI GCNNNNNNNGC 4 cut(s) 302, 878, 1414, 1683
BstNI CCWGG 3 cut(s) 347, 1001, 1043
BstNSI RCATGY 3 cut(s) 203, 703, 1020
BstSCI CCNGG 8 cut(s) 345, 516, 999, 1041, 1491, 1492, 1516, 1517
BstSFI CTRYAG 3 cut(s) 39, 560, 1467
BstSLI GKGCMC 1 cut(s) 1518
BstUI CGCG 1 cut(s) 1193
BstV1I GCAGC 4 cut(s) 762, 868, 2058, 2227
BstV2I GAAGAC 1 cut(s) 16
BstX2I RGATCY 1 cut(s) 1310
BstXI CCANNNNNNTGG 1 cut(s) 901
BstYI RGATCY 1 cut(s) 1310
BsuRI GGCC 4 cut(s) 327, 488, 1497, 1686
BtsCI GGATG 2 cut(s) 910, 1832
BtsI GCAGTG 2 cut(s) 525, 2142
BtsIMutI CAGTG 6 cut(s) 525, 658, 955, 1043, 1749, 2142
BveI ACCTGC 2 cut(s) 1093, 2113
Cac8I GCNNGC 2 cut(s) 201, 1945
CaiI CAGNNNCTG 1 cut(s) 1042
CciI TCATGA 1 cut(s) 2175
CfoI GCGC 3 cut(s) 954, 1193, 1258
Cfr10I RCCGGY 2 cut(s) 872, 1996
Cfr13I GGNCC 2 cut(s) 1495, 1685
Cfr9I CCCGGG 2 cut(s) 1492, 1517
CseI GACGC 1 cut(s) 456
Csp6I GTAC 4 cut(s) 6, 168, 697, 703
CviQI GTAC 4 cut(s) 6, 168, 697, 703
DdeI CTNAG 4 cut(s) 317, 926, 1142, 2116
DpnI GATC 6 cut(s) 436, 775, 1312, 1737, 2149, 2174
DpnII GATC 6 cut(s) 434, 773, 1310, 1735, 2147, 2172
DriI GACNNNNNGTC 1 cut(s) 264
EaeI YGGCCR 1 cut(s) 325
Eam1104I CTCTTC 2 cut(s) 295, 557
Eam1105I GACNNNNNGTC 1 cut(s) 264
EarI CTCTTC 2 cut(s) 295, 557
Eco24I GRGCYC 1 cut(s) 298
Eco31I GGTCTC 3 cut(s) 56, 1468, 1965
Eco32I GATATC 2 cut(s) 49, 1120
Eco88I CYCGRG 3 cut(s) 1492, 1517, 1687
EcoRI GAATTC 3 cut(s) 426, 1554, 1790
EcoRII CCWGG 3 cut(s) 345, 999, 1041
EcoRV GATATC 2 cut(s) 49, 1120
EcoT22I ATGCAT 2 cut(s) 130, 252
EcoT38I GRGCYC 1 cut(s) 298
Esp3I CGTCTC 1 cut(s) 251
FaqI GGGAC 7 cut(s) 43, 500, 586, 1378, 1634, 1760, 2116
FauNDI CATATG 2 cut(s) 1918, 2273
FbaI TGATCA 1 cut(s) 2172
FblI GTMKAC 1 cut(s) 1107
Fnu4HI GCNGC 4 cut(s) 751, 882, 2072, 2241
FokI GGATG 2 cut(s) 917, 1819
FriOI GRGCYC 1 cut(s) 298
Fsp4HI GCNGC 4 cut(s) 751, 882, 2072, 2241
FspBI CTAG 5 cut(s) 495, 797, 1247, 1509, 2091
GlaI GCGC 3 cut(s) 953, 1192, 1257
GluI GCNGC 4 cut(s) 751, 882, 2072, 2241
GsuI CTGGAG 1 cut(s) 2199
HaeIII GGCC 4 cut(s) 327, 488, 1497, 1686
HapII CCGG 7 cut(s) 518, 873, 1493, 1518, 1881, 1997, 2017
HgaI GACGC 1 cut(s) 456
HhaI GCGC 3 cut(s) 954, 1193, 1258
Hin6I GCGC 3 cut(s) 952, 1191, 1256
HinP1I GCGC 3 cut(s) 952, 1191, 1256
HindIII AAGCTT 1 cut(s) 314
HinfI GANTC 4 cut(s) 287, 574, 1283, 1894
HpaII CCGG 7 cut(s) 518, 873, 1493, 1518, 1881, 1997, 2017
HphI GGTGA 5 cut(s) 1333, 1592, 1799, 1982, 2011
Hpy166II GTNNAC 2 cut(s) 789, 1108
Hpy8I GTNNAC 2 cut(s) 789, 1108
Hpy99I CGWCG 2 cut(s) 472, 614
HpyAV CCTTC 6 cut(s) 94, 155, 804, 986, 1633, 1832
HpyCH4III ACNGT 1 cut(s) 67
HpyCH4IV ACGT 3 cut(s) 21, 243, 2052
HpyF10VI GCNNNNNNNGC 4 cut(s) 302, 878, 1414, 1683
HpyF3I CTNAG 4 cut(s) 317, 926, 1142, 2116
HpySE526I ACGT 3 cut(s) 21, 243, 2052
HspAI GCGC 3 cut(s) 952, 1191, 1256
Ksp22I TGATCA 1 cut(s) 2172
Kzo9I GATC 6 cut(s) 434, 773, 1310, 1735, 2147, 2172
LguI GCTCTTC 1 cut(s) 295
LmnI GCTCC 4 cut(s) 16, 604, 1361, 2068
Lsp1109I GCAGC 4 cut(s) 762, 868, 2058, 2227
LweI GCATC 4 cut(s) 327, 539, 1963, 2077
MaeI CTAG 5 cut(s) 495, 797, 1247, 1509, 2091
MaeII ACGT 3 cut(s) 21, 243, 2052
MaeIII GTNAC 6 cut(s) 1153, 1225, 1242, 1504, 1546, 2141
MalI GATC 6 cut(s) 436, 775, 1312, 1737, 2149, 2174
MboI GATC 6 cut(s) 434, 773, 1310, 1735, 2147, 2172
MboII GAAGA 9 cut(s) 16, 312, 544, 1444, 1558, 1580, 1775, 1817, 1854
MfeI CAATTG 1 cut(s) 372
MflI RGATCY 1 cut(s) 1310
MhlI GDGCHC 2 cut(s) 298, 1518
MlsI TGGCCA 1 cut(s) 327
MluNI TGGCCA 1 cut(s) 327
MlyI GAGTC 2 cut(s) 568, 1292
MmeI TCCRAC 4 cut(s) 423, 450, 716, 1640
Mox20I TGGCCA 1 cut(s) 327
Mph1103I ATGCAT 2 cut(s) 130, 252
MscI TGGCCA 1 cut(s) 327
MseI TTAA 4 cut(s) 102, 140, 863, 1698
MslI CAYNNNNRTG 6 cut(s) 190, 383, 698, 835, 1319, 1799
Msp20I TGGCCA 1 cut(s) 327
MspA1I CMGCKG 2 cut(s) 146, 1871
MspI CCGG 7 cut(s) 518, 873, 1493, 1518, 1881, 1997, 2017
MspR9I CCNGG 8 cut(s) 347, 518, 1001, 1043, 1493, 1494, 1518, 1519
MunI CAATTG 1 cut(s) 372
Mva1269I GAATGC 1 cut(s) 1224
MvaI CCWGG 3 cut(s) 347, 1001, 1043
MvnI CGCG 1 cut(s) 1193
MwoI GCNNNNNNNGC 4 cut(s) 302, 878, 1414, 1683
NciI CCSGG 5 cut(s) 518, 1493, 1494, 1518, 1519
NdeI CATATG 2 cut(s) 1918, 2273
NdeII GATC 6 cut(s) 434, 773, 1310, 1735, 2147, 2172
NlaIV GGNNCC 5 cut(s) 361, 1312, 1426, 1515, 1879
NmuCI GTSAC 3 cut(s) 1242, 1546, 2141
NsiI ATGCAT 2 cut(s) 130, 252
NspI RCATGY 3 cut(s) 203, 703, 1020
OliI CACNNNNGTG 1 cut(s) 190
PaeI GCATGC 1 cut(s) 203
PagI TCATGA 1 cut(s) 2175
PaqCI CACCTGC 1 cut(s) 2113
PciI ACATGT 1 cut(s) 699
PciSI GCTCTTC 1 cut(s) 295
PctI GAATGC 1 cut(s) 1224
PfeI GAWTC 2 cut(s) 287, 1894
PflMI CCANNNNNTGG 1 cut(s) 1874
PkrI GCNGC 4 cut(s) 752, 883, 2073, 2242
PleI GAGTC 2 cut(s) 568, 1291
PpsI GAGTC 2 cut(s) 568, 1291
Ppu21I YACGTR 1 cut(s) 2053
PscI ACATGT 1 cut(s) 699
PsiI TTATAA 1 cut(s) 1031
Psp6I CCWGG 3 cut(s) 345, 999, 1041
PspGI CCWGG 3 cut(s) 345, 999, 1041
PspN4I GGNNCC 5 cut(s) 361, 1312, 1426, 1515, 1879
PspPI GGNCC 2 cut(s) 1495, 1685
PstI CTGCAG 2 cut(s) 43, 1471
PstNI CAGNNNCTG 1 cut(s) 1042
PsuI RGATCY 1 cut(s) 1310
PvuII CAGCTG 2 cut(s) 146, 1871
RsaI GTAC 4 cut(s) 7, 169, 698, 704
RsaNI GTAC 4 cut(s) 6, 168, 697, 703
RseI CAYNNNNRTG 6 cut(s) 190, 383, 698, 835, 1319, 1799
SapI GCTCTTC 1 cut(s) 295
SaqAI TTAA 4 cut(s) 102, 140, 863, 1698
SatI GCNGC 4 cut(s) 751, 882, 2072, 2241
Sau3AI GATC 6 cut(s) 434, 773, 1310, 1735, 2147, 2172
Sau96I GGNCC 2 cut(s) 1495, 1685
ScaI AGTACT 1 cut(s) 7
SchI GAGTC 2 cut(s) 568, 1292
ScrFI CCNGG 8 cut(s) 347, 518, 1001, 1043, 1493, 1494, 1518, 1519
SduI GDGCHC 2 cut(s) 298, 1518
SfaNI GCATC 4 cut(s) 327, 539, 1963, 2077
SfcI CTRYAG 3 cut(s) 39, 560, 1467
SgrAI CRCCGGYG 1 cut(s) 1996
SmaI CCCGGG 2 cut(s) 1494, 1519
SmiMI CAYNNNNRTG 6 cut(s) 190, 383, 698, 835, 1319, 1799
SmlI CTYRAG 1 cut(s) 119
SmoI CTYRAG 1 cut(s) 119
SpeI ACTAGT 1 cut(s) 1246
SphI GCATGC 1 cut(s) 203
SrfI GCCCGGGC 1 cut(s) 1494
SsiI CCGC 1 cut(s) 2042
SspI AATATT 3 cut(s) 721, 1302, 2230
SspMI CTAG 5 cut(s) 495, 797, 1247, 1509, 2091
StyD4I CCNGG 8 cut(s) 345, 516, 999, 1041, 1491, 1492, 1516, 1517
TaaI ACNGT 1 cut(s) 67
TaiI ACGT 3 cut(s) 24, 246, 2055
TaqI TCGA 5 cut(s) 298, 1317, 1440, 1781, 1980
TaqII GACCGA 2 cut(s) 1276, 1947
TatI WGTACW 3 cut(s) 5, 696, 702
TfiI GAWTC 2 cut(s) 287, 1894
Tru1I TTAA 4 cut(s) 102, 140, 863, 1698
Tru9I TTAA 4 cut(s) 102, 140, 863, 1698
TscAI CASTG 6 cut(s) 532, 665, 962, 1043, 1756, 2142
TseFI GTSAC 3 cut(s) 1242, 1546, 2141
TseI GCWGC 4 cut(s) 750, 881, 2071, 2240
Tsp45I GTSAC 3 cut(s) 1242, 1546, 2141
TspGWI ACGGA 2 cut(s) 1437, 2160
TspMI CCCGGG 2 cut(s) 1492, 1517
TspRI CASTG 6 cut(s) 532, 665, 962, 1043, 1756, 2142
Van91I CCANNNNNTGG 1 cut(s) 1874
XbaI TCTAGA 1 cut(s) 494
XceI RCATGY 3 cut(s) 203, 703, 1020
XmaI CCCGGG 2 cut(s) 1492, 1517
XmiI GTMKAC 1 cut(s) 1107
XspI CTAG 5 cut(s) 495, 797, 1247, 1509, 2091
ZrmI AGTACT 1 cut(s) 7
Zsp2I ATGCAT 2 cut(s) 130, 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.