MD14G1239000.v1.1

NF-X1-type zinc finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0012624
Physical Location & Seq
Forward (+)
31813727 .. 31816178
2452 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1239000.v1.1.491

Sequence Viewer

Length: 1209 bp
ATGGTTCAACTGAGTACAGCTCCCTTATACGTGTTCGGGACATTGAACGCAGACGATATCAAATCAGACGAGACCGTTTATGAGCTATTCTCCTTCATTTTCCGGACTATTAACATCATTTCTTTACTCAAGTATGCATTTATAGTATTAGCAGCTGATGACAATGGAGAAGGTGGTACTTTTGCTTTGTATTCACTCCTGTGTAGGCATGCTAAAGTTGGCCTGCTTCCAAGTGACAGTAGTGCTAATGACGTTATGCTCTATGAGGCGGGAAGCCCGTACAAGACAAAGATGGAATCAAGGGCTAGAAGAGCCATAGAAAAACATAGAAGTAGCCACTATTTGATGTTGTTCCTAGCTCTTTTTGGTTCCTGCATGACAATCGGCGTTGGGGTGTTTACCCCAGCTCTTTCTGGTTTGGCATTCCTCATTACCTTTTCTAAATATTACTGGAAATTTATAAGAAAGTTTACTTTCTGCATCGAATTTATGCCGAATTTGATCATATGTAGCATTATTTCATCCAGTATTCAAAGATCATTGTTGGATATTGAATCCAGATACGTCTCGGTTCCCACTGCATCTGCTATATTGGTGTGCCTCTTCACACTACAGCACTATGGGACTCGTAAAATTGGGTTTATGTTTGCTCCTGTCATCGTTGTTTGGCTGTTATTTATCGGTGGAGGGGGTGCATATAACATTTTCCACTGGAACAGAGAAATTATCCGTGCTACCTCCCCTATATACATTTACAGGTTCTTTAGGAAGATTCACAGCAAAACTTGGAGGTCATTAGGCAGCATCGCTTTATGTGTAGCAGTATTTTCACTTCTTGCTTCAGTTGTGGGAAGCCAGGCAACTATAACAGCATGCTTCTCCATCATAAATCAGTGCCTGGCACTTGGTTGTTTTCCGAGAGTAAAAGTTATTCATACATCAGATGAGATACACGGGCAGGTTTACATTCCTGATATCAACTGGCTATTGATGGTTCTAAGCCTCGTTGTTACGATTGGTTTCCATGATATTATCAAAATTGGTAGCGCGACAGGTTTGGCTATAAGTTCTGGAATGCTTGTAACGACTTGTCTCATGTCACTTGTAATTGCGCTATACTGGGAGAAGAGTTTGTTTGAGTCCGTCAGCTGTCTAATATTCTTTTGGCTGTGGGCTGTGGGCTGTGGGCTGTGGGCTGTGTGTGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000122 GO:0000976 GO:0000977 GO:0000981 GO:0000982 GO:0001012 GO:0001067 GO:0001078 GO:0001227 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006082 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009314 GO:0009416 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009636 GO:0009642 GO:0009651 GO:0009696 GO:0009697 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010188 GO:0010310 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010817 GO:0016053 GO:0016070 GO:0016999 GO:0017000 GO:0017144 GO:0018130 GO:0018958 GO:0019219 GO:0019222 GO:0019438 GO:0019752 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032774 GO:0032787 GO:0034641 GO:0034645 GO:0034654 GO:0042221 GO:0042445 GO:0042446 GO:0042537 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0046189 GO:0046394 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051193 GO:0051252 GO:0051253 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0065008 GO:0071704 GO:0072330 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0098542 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901615 GO:1901617 GO:1902679 GO:1903506 GO:1903507 GO:1990837 GO:2000112 GO:2000113 GO:2000377 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko05165 map05165
Pfam Domains
Protein Families
K_trans R3H zf-NF-X1

Protein Analysis

403

Amino Acids

44.83

Weight (kDa)

8.81

Isoelectric Point (pI)

40.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
K_trans PF02705 4 - 138 3.7e-21 K+ potassium transporter integral membrane domain
K_trans PF02705 185 - 274 3.5e-21 K+ potassium transporter integral membrane domain
K_trans PF02705 275 - 391 1.8e-32 K+ potassium transporter integral membrane domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 461
Acc36I ACCTGC 1 cut(s) 949
AccII CGCG 1 cut(s) 1049
AccIII TCCGGA 1 cut(s) 102
AciI CCGC 1 cut(s) 269
AcsI RAATTY 3 cut(s) 455, 485, 496
AcuI CTGAAG 1 cut(s) 825
AfaI GTAC 3 cut(s) 16, 178, 281
AflIII ACRYGT 1 cut(s) 30
AgsI TTSAA 4 cut(s) 8, 46, 533, 554
AjnI CCWGG 2 cut(s) 855, 897
AleI CACNNNNGTG 1 cut(s) 199
AluBI AGCT 6 cut(s) 20, 85, 155, 359, 407, 1149
AluI AGCT 6 cut(s) 20, 85, 155, 359, 407, 1149
Alw26I GTCTC 3 cut(s) 65, 571, 1097
AlwNI CAGNNNCTG 1 cut(s) 898
Aor13HI TCCGGA 1 cut(s) 102
AoxI GGCC 1 cut(s) 220
ApeKI GCWGC 2 cut(s) 152, 801
ApoI RAATTY 3 cut(s) 455, 485, 496
AspLEI GCGC 2 cut(s) 1049, 1114
BarI GAAGNNNNNNTAC 2 cut(s) 816, 848
BbvI GCAGC 2 cut(s) 164, 813
BccI CCATC 3 cut(s) 286, 890, 985
BcgI CGANNNNNNTGC 2 cut(s) 364, 398
BciT130I CCWGG 2 cut(s) 857, 899
BclI TGATCA 1 cut(s) 501
BcoDI GTCTC 3 cut(s) 65, 571, 1097
BfaI CTAG 2 cut(s) 306, 356
BfmI CTRYAG 1 cut(s) 611
BfuAI ACCTGC 1 cut(s) 949
BisI GCNGC 2 cut(s) 153, 802
BlsI GCNGC 2 cut(s) 154, 803
Bme1390I CCNGG 2 cut(s) 857, 899
BmiI GGNNCC 2 cut(s) 370, 573
BmrFI CCNGG 2 cut(s) 857, 899
BmrI ACTGGG 1 cut(s) 1129
BmsI GCATC 3 cut(s) 489, 590, 813
BmuI ACTGGG 1 cut(s) 1129
BplI GAGNNNNNCTC 3 cut(s) 36, 74, 106
BpuEI CTTGAG 1 cut(s) 113
BsaAI YACGTR 1 cut(s) 31
BsaI GGTCTC 1 cut(s) 65
BsaWI WCCGGW 1 cut(s) 102
Bse1I ACTGG 5 cut(s) 455, 525, 716, 986, 1124
BseAI TCCGGA 1 cut(s) 102
BseBI CCWGG 2 cut(s) 857, 899
BseGI GGATG 1 cut(s) 521
BseNI ACTGG 5 cut(s) 455, 525, 716, 986, 1124
BseXI GCAGC 2 cut(s) 164, 813
BseYI CCCAGC 1 cut(s) 403
Bsh1236I CGCG 1 cut(s) 1049
BshFI GGCC 1 cut(s) 222
BsiSI CCGG 1 cut(s) 103
BslFI GGGAC 2 cut(s) 52, 637
BsmAI GTCTC 3 cut(s) 65, 571, 1097
BsmBI CGTCTC 1 cut(s) 571
BsmFI GGGAC 2 cut(s) 52, 637
BsmI GAATGC 2 cut(s) 422, 1080
BsnI GGCC 1 cut(s) 222
Bso31I GGTCTC 1 cut(s) 65
Bsp13I TCCGGA 1 cut(s) 102
Bsp143I GATC 2 cut(s) 501, 536
BspACI CCGC 1 cut(s) 269
BspANI GGCC 1 cut(s) 222
BspEI TCCGGA 1 cut(s) 102
BspFNI CGCG 1 cut(s) 1049
BspLI GGNNCC 2 cut(s) 370, 573
BspMI ACCTGC 1 cut(s) 949
BspQI GCTCTTC 1 cut(s) 304
BspTNI GGTCTC 1 cut(s) 65
BsrI ACTGG 5 cut(s) 455, 525, 716, 986, 1124
BssMI GATC 2 cut(s) 501, 536
Bst2UI CCWGG 2 cut(s) 857, 899
Bst4CI ACNGT 2 cut(s) 76, 239
Bst6I CTCTTC 3 cut(s) 304, 608, 1121
BstBAI YACGTR 1 cut(s) 31
BstC8I GCNNGC 3 cut(s) 210, 224, 874
BstDEI CTNAG 2 cut(s) 11, 998
BstF5I GGATG 1 cut(s) 521
BstFNI CGCG 1 cut(s) 1049
BstHHI GCGC 2 cut(s) 1049, 1114
BstKTI GATC 2 cut(s) 504, 539
BstMAI GTCTC 3 cut(s) 65, 571, 1097
BstMBI GATC 2 cut(s) 501, 536
BstMWI GCNNNNNNNGC 1 cut(s) 311
BstNI CCWGG 2 cut(s) 857, 899
BstNSI RCATGY 2 cut(s) 212, 876
BstSCI CCNGG 2 cut(s) 855, 897
BstSFI CTRYAG 1 cut(s) 611
BstUI CGCG 1 cut(s) 1049
BstV1I GCAGC 2 cut(s) 164, 813
BsuRI GGCC 1 cut(s) 222
BtgZI GCGATG 1 cut(s) 790
BtsCI GGATG 1 cut(s) 521
BtsI GCAGTG 1 cut(s) 576
BtsIMutI CAGTG 3 cut(s) 576, 709, 899
BveI ACCTGC 1 cut(s) 949
Cac8I GCNNGC 3 cut(s) 210, 224, 874
CaiI CAGNNNCTG 1 cut(s) 898
CfoI GCGC 2 cut(s) 1049, 1114
Csp6I GTAC 3 cut(s) 15, 177, 280
CviAII CATG 5 cut(s) 209, 376, 873, 1025, 1096
CviQI GTAC 3 cut(s) 15, 177, 280
DdeI CTNAG 2 cut(s) 11, 998
DpnI GATC 2 cut(s) 503, 538
DpnII GATC 2 cut(s) 501, 536
Eam1104I CTCTTC 3 cut(s) 304, 608, 1121
EarI CTCTTC 3 cut(s) 304, 608, 1121
Eco31I GGTCTC 1 cut(s) 65
Eco32I GATATC 2 cut(s) 58, 976
Eco57I CTGAAG 1 cut(s) 825
EcoRII CCWGG 2 cut(s) 855, 897
EcoRV GATATC 2 cut(s) 58, 976
EcoT22I ATGCAT 1 cut(s) 139
Esp3I CGTCTC 1 cut(s) 571
FaeI CATG 5 cut(s) 212, 379, 876, 1028, 1099
FaqI GGGAC 2 cut(s) 52, 637
FatI CATG 5 cut(s) 208, 375, 872, 1024, 1095
FauI CCCGC 1 cut(s) 262
FauNDI CATATG 1 cut(s) 506
FbaI TGATCA 1 cut(s) 501
Fnu4HI GCNGC 2 cut(s) 153, 802
FokI GGATG 1 cut(s) 508
Fsp4HI GCNGC 2 cut(s) 153, 802
FspBI CTAG 2 cut(s) 306, 356
GlaI GCGC 2 cut(s) 1048, 1113
GluI GCNGC 2 cut(s) 153, 802
GsaI CCCAGC 1 cut(s) 407
HaeIII GGCC 1 cut(s) 222
HapII CCGG 1 cut(s) 103
HhaI GCGC 2 cut(s) 1049, 1114
Hin1II CATG 5 cut(s) 212, 379, 876, 1028, 1099
Hin6I GCGC 2 cut(s) 1047, 1112
HinP1I GCGC 2 cut(s) 1047, 1112
HinfI GANTC 5 cut(s) 296, 554, 625, 772, 1139
HpaII CCGG 1 cut(s) 103
Hpy166II GTNNAC 3 cut(s) 399, 471, 964
Hpy188I TCNGA 3 cut(s) 67, 918, 943
Hpy188III TCNNGA 5 cut(s) 37, 103, 558, 971, 1071
Hpy8I GTNNAC 3 cut(s) 399, 471, 964
HpyAV CCTTC 2 cut(s) 103, 164
HpyCH4III ACNGT 2 cut(s) 76, 239
HpyCH4IV ACGT 3 cut(s) 30, 252, 564
HpyCH4V TGCA 5 cut(s) 137, 375, 480, 581, 695
HpyF10VI GCNNNNNNNGC 1 cut(s) 311
HpyF3I CTNAG 2 cut(s) 11, 998
HpySE526I ACGT 3 cut(s) 30, 252, 564
Hsp92II CATG 5 cut(s) 212, 379, 876, 1028, 1099
HspAI GCGC 2 cut(s) 1047, 1112
Kpn2I TCCGGA 1 cut(s) 102
Ksp22I TGATCA 1 cut(s) 501
Kzo9I GATC 2 cut(s) 501, 536
LguI GCTCTTC 1 cut(s) 304
LmnI GCTCC 2 cut(s) 25, 655
Lsp1109I GCAGC 2 cut(s) 164, 813
LweI GCATC 3 cut(s) 489, 590, 813
MaeI CTAG 2 cut(s) 306, 356
MaeII ACGT 3 cut(s) 30, 252, 564
MaeIII GTNAC 4 cut(s) 233, 1009, 1081, 1098
MalI GATC 2 cut(s) 503, 538
MboI GATC 2 cut(s) 501, 536
MboII GAAGA 4 cut(s) 321, 595, 781, 1138
MluCI AATT 7 cut(s) 455, 485, 496, 633, 723, 1038, 1107
MlyI GAGTC 2 cut(s) 619, 1148
MmeI TCCRAC 1 cut(s) 525
MnlI CCTC 7 cut(s) 259, 437, 611, 680, 748, 783, 1013
Mph1103I ATGCAT 1 cut(s) 139
MroI TCCGGA 1 cut(s) 102
MseI TTAA 1 cut(s) 111
MslI CAYNNNNRTG 1 cut(s) 199
MspA1I CMGCKG 2 cut(s) 155, 1149
MspI CCGG 1 cut(s) 103
MspR9I CCNGG 2 cut(s) 857, 899
Mva1269I GAATGC 2 cut(s) 422, 1080
MvaI CCWGG 2 cut(s) 857, 899
MvnI CGCG 1 cut(s) 1049
MwoI GCNNNNNNNGC 1 cut(s) 311
NdeI CATATG 1 cut(s) 506
NdeII GATC 2 cut(s) 501, 536
NlaIII CATG 5 cut(s) 212, 379, 876, 1028, 1099
NlaIV GGNNCC 2 cut(s) 370, 573
NmuCI GTSAC 2 cut(s) 233, 1098
NsiI ATGCAT 1 cut(s) 139
NspI RCATGY 2 cut(s) 212, 876
OliI CACNNNNGTG 1 cut(s) 199
PaeI GCATGC 2 cut(s) 212, 876
PciSI GCTCTTC 1 cut(s) 304
PctI GAATGC 2 cut(s) 422, 1080
PfeI GAWTC 3 cut(s) 296, 554, 772
PkrI GCNGC 2 cut(s) 154, 803
PleI GAGTC 2 cut(s) 619, 1147
PpsI GAGTC 2 cut(s) 619, 1147
Ppu21I YACGTR 1 cut(s) 31
PsiI TTATAA 1 cut(s) 461
Psp6I CCWGG 2 cut(s) 855, 897
PspFI CCCAGC 1 cut(s) 403
PspGI CCWGG 2 cut(s) 855, 897
PspN4I GGNNCC 2 cut(s) 370, 573
PstNI CAGNNNCTG 1 cut(s) 898
PvuII CAGCTG 2 cut(s) 155, 1149
RsaI GTAC 3 cut(s) 16, 178, 281
RsaNI GTAC 3 cut(s) 15, 177, 280
RseI CAYNNNNRTG 1 cut(s) 199
SapI GCTCTTC 1 cut(s) 304
SaqAI TTAA 1 cut(s) 111
SatI GCNGC 2 cut(s) 153, 802
Sau3AI GATC 2 cut(s) 501, 536
SchI GAGTC 2 cut(s) 619, 1148
ScrFI CCNGG 2 cut(s) 857, 899
SfaNI GCATC 3 cut(s) 489, 590, 813
SfcI CTRYAG 1 cut(s) 611
SmiMI CAYNNNNRTG 1 cut(s) 199
SmlI CTYRAG 1 cut(s) 128
SmoI CTYRAG 1 cut(s) 128
SphI GCATGC 2 cut(s) 212, 876
Sse9I AATT 7 cut(s) 455, 485, 496, 633, 723, 1038, 1107
SsiI CCGC 1 cut(s) 269
SspI AATATT 2 cut(s) 446, 1158
SspMI CTAG 2 cut(s) 306, 356
StyD4I CCNGG 2 cut(s) 855, 897
TaaI ACNGT 2 cut(s) 76, 239
TaiI ACGT 3 cut(s) 33, 255, 567
TaqI TCGA 1 cut(s) 483
TasI AATT 7 cut(s) 455, 485, 496, 633, 723, 1038, 1107
TatI WGTACW 1 cut(s) 14
TfiI GAWTC 3 cut(s) 296, 554, 772
Tru1I TTAA 1 cut(s) 111
Tru9I TTAA 1 cut(s) 111
TscAI CASTG 3 cut(s) 583, 716, 899
TseFI GTSAC 2 cut(s) 233, 1098
TseI GCWGC 2 cut(s) 152, 801
Tsp45I GTSAC 2 cut(s) 233, 1098
TspDTI ATGAA 3 cut(s) 85, 510, 923
TspGWI ACGGA 2 cut(s) 719, 1132
TspRI CASTG 3 cut(s) 583, 716, 899
XapI RAATTY 3 cut(s) 455, 485, 496
XceI RCATGY 2 cut(s) 212, 876
XspI CTAG 2 cut(s) 306, 356
Zsp2I ATGCAT 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.