Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0006728

Physical Location & Seq

Genomic Coordinates Forward (+)

36592124 .. 36599402

Gene Structure 7279 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1234900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3810 bp

ATGAGAGTAGTGAGTGGAATATATTCAAGTTATTCCCATGAGCATCAAAATCTAAAACTAAACGAAGCTTTTGGTATTTGGACCACCTCCACCTCGATTCAGGAGACTACGCCTCCCTCCTCCCAAAAGCCTACAAAGACGAGAAACAAAAGAAGCAGCGGCGCCGGCAGCAACCGGTCACTCCCATTCCCACCAGTCTGCCTGACAGTAGAAGACAAAATGGCACCAGTCGTTGAGCTTAATACAAGGAGGCTGAAAATGGCCGACTCAAAGTCCGTTGACGTTATTCTGGACTTTCTGCGGAAGAATAGGTTTTCAAGGGCAGAGGCAGCTCTTCGAAGTGAGCTTGGTAATCGTCCTGATTTGAATGGTTTCCTTCAGAAGCTTACTTTTGAGGATAAGGATTCTAGTCATTCGTCGGAAGCTGAAAACAGGGACAAGCTAGTGGTGGAAAATCAAGGCTTGGGATCTCGGAATGGTGATGAAGTTTCTCAGGAACTTATAGTGAAAGAGATTGAGTGTGGAACTGGTAGAAATGGGTCTGAAAGCAAATGGAAAAATGCTACTTCTATTGGGGAGTGGAATAAATCAGTTGATGTGGCTGGGACAAATCACAAGAGCTTTGCATTCTCTAAAGGTTTAGAGGAGACCGTGCTTGATTTATATTCTTGGCAGTCCAGTCCGAGCAATGGACCCGCAGAACCTTATCAAAGTGATGGTGGTGGTATTATCAGCAACTTCCCAGAGCCTCAAATATCCCAGCAATCAAAGAATCATACAACTGAAGTTCCCAATTCAGGTAAAGCCATTTTGAAATATGGAGAAGAGATCAGCTTTTCTGGTGAAAAGAAAACCTCATGGTCTGGAAGTACTAGCAAACCTAGTGTGGAATTCAAGTATGACAGAACCCAAACAAGTGAACCTAAGGAACTTGACCAACAACTTAAGACGAGTACCACATTCTTCAAAGAAAATGCAGCAGAGAATCCTTGGCCAAGAATCGAAGAACCAACAAATCCACCTTCAGAGATGTGGAAAGATTGTTCTGTCAAGACCGTGTTTCCTTTCTCCAAGGGTGATGTGTTAACGAGTTATGATAGTGCTTCTGGTTCTGACAAAAAAGAAGGAAAGAGAAAAAATGAGTTGACTGATATTAGGGCAACAATAAAGGAGCAGGTTGATGAGGTGGGAAGAGCTTTATACTTAAGCCAGTCACAAGGGAGTTCTGAGCAAAAGACCATTAGTAATTTAATTTTTCCTATTTTGTCTGAGAATCAAAAGGAAGAGTTCCCTAGGCTGCCACCAGTCAAACTCAAGTCAGAGGACAAACCATTGAATGTTAATTGGGAGGAAAAGTTTGAGCGAGACGGACCTGGCCCAAAACTCAGCTCTGCTGACAATGCTCTTCTTATTGGGTCATATTTGGATGTACCTATTGGACAAGAAATCAACTCTTCAGATTATGGTTCAGGTGGAAAAAGACCTGTAGGAGGCAGTTGGCTGTCTGTAAGTCAGGGGATCACAGAGGACGCATCTGATCTGGTTTCTGGTTTTGCTACCGTTGGTGATGGATTGAGTGAAACGATTGACTACCCCTATTGGGACTCTGATGAATATGATGATGATGATGATGTTGGATACATGAGACAACCTATTGAAGATGAGGCATGGTTTCTGGCCCATGAAATTGATTATCCTAGTGACAATGAAAAGGGAACAGGTCATGGGAGTGTTCCAGATCCACAGGAAAGGGGCCCAACCAAAGAGGAGGATGATGATCAATCTTTTGCTGAGGAGGATTCTTATTTATCTGGTGAGCGATACTTTCAAGCGAAAAATGTTGAACCAATTATAACTTCAGATGATCCTATGGGATTATCAGTGACTGAATTGTATGGGAGAACCGATGAGAATGATCTGATAGCTCAATATGATGGACAGTTGATGGATGAAGAGGAGCTTAATTTAATGCGTTCAGAACCTGTCTGGCAGGGCTTTGTCACTCAGACCAATGAACTCATCATGTTAGGGGATGGGAAAGATGTGAATGAGAATGGAAGGCCATGTCTGGAAGAGGTTTGTGTGGATGACGACCAGCTTGGTTCAGTAAGATCAATAGGTGTGGGAATCAATAGCGAAGCTGCTGATATCGGCAGTGAAGTACGGGAAAGTTTGATTGGAGGCAGTAGTGAAGGGGATCTAGAATACTTTCAGGACCATGATGTAGGAATTGGTGGGCCACGGAAGAATCATCAGGACTCAGATAAGAAACATATTGATAGACTTGAGAAGGACAAGAAGAAAACTAGTAAACATGAGGCAAATAAATATACAGTTGAGAACAATGATGGTGCTTTTAGACAGAAGAAAATTCATTCAGAAGGGGGATTTTCATTCCCCCCACCACTGAGAGATGGGCAATTGGTGCAGGCAAGCTCTAGTAAACCTTTATGGTCGAACAACTGCAATGCTGTTGTAACTGAAGAACCTGATGATGATAAAATGCTTGCTTCGTGGAGGGAGAAAAGTAACGAGTCCTCACCTAGAATGAGTTCTAGGGATGAAAATAATGCTAATGCTGTTAGATCCACAAACTCGACTCCCTCAACGCTCTCTAATTATGCTTATGCTGAAAGAGAGCATGCCAAGCAAGAAGAAGATGAGAAAATTGCTGCTGTTAGGGAGGAAGATAGGGGGGCATCACTTGAGGATGAAGAGGCGGCTGCTGTGCAAGAGCAAGTAAGGCAAATAAAGGCTCAAGAGGAAGAGTTTGAGACCTTCAACCTCAAGATTGTGCACAGGAAAAACAGAACTGGCTTTGAGGAGGACAAAAATTTTCATGTTGTTTTGAATTCTGTGTTAGCTGGCCGCTATCATGTCACCGAATATCTTGGATCAGCTGCATTCAGCAAAGCTATTCAAGCTCATGATCTACATACAGGCATGGATGTATGTGTGAAAATTATAAAGAACAACAAAGACTTCTTTGATCAGAGCCTTGATGAGATAAAGCTTCTCAAATACGTCAATAAGCATGATCCTGGTGACAAGTACCACATTCTTCGTTTGTACGATTACTTCTACTATCGAGAACATTTGTTAATAGTATGTGAACTTCTCAAAGCAAACTTGTATGAATTTCATAAGTTTAATCGGGAGTCAGGAGGGGAGGTCTACTTCACAATGCCACGACTGCAGTCAATTACGATTCAGTGTCTAGAAGCGCTTCAGTTTTTGCATGGCCTTGGCATGATACATTGTGACTTGAAACCTGAGAATATACTGGTAAAAAGCTACAGTAGATGTGAGGTCAAAGTCATTGATCTTGGGAGTAGTTGCTTTGAGACGGATCATCTCTGCTCCTATGTTCAGTCCAGATCCTATCGTGCTCCTGAGGTTATCTTGGGACTTCCATATGATAAGAAGATAGATATATGGTCACTTGGCTGCATCTTGGCCGAACTTTGTACTGGCAATGTACTCTTCCAGAATGATTCACCTGCAACTTTACTTGCACGGGTGATGGGAATCATTTCCCCTATTGATCAAGGCATGCTAGCGAAAGGGCGTGATACATACAAATACTTCACAAAAAATCACATGCTTTATGAACGGAATCAGGAAACTAACAGACTGGAATACCTGATACCAAAGAAGACATCCTTGAGGCACAGGCTTCCAATGGGGGACCAAGGCTTCATTGACTTCATTGCTCATCTGCTTGAGATAAACCCGAAGAAGCGCCCTTCTGCATCTGACGCTCTGAAGCACCCGTGGCTATCATATCCTTATGAACCTATATCATCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

1270

Amino Acids

142.62

Weight (kDa)

5.0

Isoelectric Point (pI)

46.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	958 - 1260	5.4e-50	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	960 - 1164	2.4e-17	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006728)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G73450 AT1G73460 AT1G73460 AT3G17750
fragaria_vesca	FvH4_5g17930 FvH4_5g17930 FvH4_5g17930
malus_domestica	MD06G1234900.v1.1 MD14G1242100.v1.1
prunus_persica	Prupe.5G239700_v2.0.a1 Prupe.5G239700_v2.0.a1 Prupe.5G239700_v2.0.a1 Prupe.5G239700_v2.0.a1
pyrus_communis	pycom06g20990 pycom14g20350
rosa_chinensis	RchiOBHm_Chr7g0177671
rosa_laevigata	RLG00000005515
rosa_multiflora	Rmu_co8343931.1_g000001 Rmu_ssc0000236.1_g000003
rosa_roxburghii	Rroxscaffold_3G00275670
rosa_rugosa	Rorug06G0408400
rosa_samantha	Rh7AG007900 Rh7BG007800 Rh7CG008300 Rh7DG008000
rosa_wichuraiana	Rw7G000660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1853, 2966
AarI	CACCTGC	1 cut(s)	3508
Acc36I	ACCTGC	2 cut(s)	1165, 3508
AccB1I	GGYRCC	2 cut(s)	161, 223
AccI	GTMKAC	1 cut(s)	3174
AciI	CCGC	5 cut(s)	159, 301, 696, 2720, 2869
AcoI	YGGCCR	4 cut(s)	261, 992, 2866, 3456
AcsI	RAATTY	5 cut(s)	890, 2370, 2833, 2851, 3137
AcuI	CTGAAG	8 cut(s)	362, 804, 1008, 1440, 1842, 2502, 3212, 3785
AcyI	GRCGYC	1 cut(s)	162
AfaI	GTAC	8 cut(s)	871, 955, 1431, 2163, 3053, 3071, 3469, 3480
AfeI	AGCGCT	1 cut(s)	3225
AfiI	CCNNNNNNNGG	4 cut(s)	689, 797, 1490, 1600
AflII	CTTAAG	2 cut(s)	944, 1204
AgeI	ACCGGT	1 cut(s)	174
AhdI	GACNNNNNGTC	1 cut(s)	271
AhlI	ACTAGT	1 cut(s)	2306
AjnI	CCWGG	2 cut(s)	1372, 3040
AjuI	GAANNNNNNNTTGG	4 cut(s)	1840, 1872, 2160, 2192
AloI	GAACNNNNNNTCC	2 cut(s)	1027, 1059
Alw21I	GWGCWC	2 cut(s)	2799, 3391
Alw26I	GTCTC	6 cut(s)	98, 641, 1359, 1639, 2768, 3338
Alw44I	GTGCAC	1 cut(s)	2795
AlwNI	CAGNNNCTG	2 cut(s)	1886, 1982
Aor51HI	AGCGCT	1 cut(s)	3225
ApaI	GGGCCC	1 cut(s)	1757
ApaLI	GTGCAC	1 cut(s)	2795
ApoI	RAATTY	5 cut(s)	890, 2370, 2833, 2851, 3137
AsiGI	ACCGGT	1 cut(s)	174
Asp700I	GAANNNNTTC	6 cut(s)	22, 371, 2208, 2551, 3225, 3532
AspA2I	CCTAGG	1 cut(s)	1292
AspLEI	GCGC	3 cut(s)	164, 3226, 3744
AsuII	TTCGAA	1 cut(s)	337
AsuNHI	GCTAGC	1 cut(s)	3556
AvaII	GGWCC	5 cut(s)	81, 692, 1370, 2215, 3688
AvrII	CCTAGG	1 cut(s)	1292
AxyI	CCTNAGG	2 cut(s)	924, 3393
BaeGI	GKGCMC	2 cut(s)	1757, 2799
BalI	TGGCCA	1 cut(s)	994
BanI	GGYRCC	2 cut(s)	161, 223
BanII	GRGCYC	1 cut(s)	1757
BarI	GAAGNNNNNNTAC	4 cut(s)	3062, 3094, 3099, 3131
BbsI	GAAGAC	2 cut(s)	219, 3662
Bbv12I	GWGCWC	2 cut(s)	2799, 3391
BbvCI	CCTCAGC	1 cut(s)	1791
BccI	CCATC	8 cut(s)	710, 1562, 1928, 1939, 2027, 2342, 2408, 3517
BciT130I	CCWGG	2 cut(s)	1374, 3042
BciVI	GTATCC	1 cut(s)	1631
BclI	TGATCA	3 cut(s)	1777, 2989, 3544
BcoDI	GTCTC	6 cut(s)	98, 641, 1359, 1639, 2768, 3338
BcuI	ACTAGT	1 cut(s)	2306
BfmI	CTRYAG	3 cut(s)	1485, 3194, 3295
BfoI	RGCGCY	3 cut(s)	165, 3227, 3745
BfrI	CTTAAG	2 cut(s)	944, 1204
BfuAI	ACCTGC	2 cut(s)	1165, 3508
BfuI	GTATCC	1 cut(s)	1631
BlnI	CCTAGG	1 cut(s)	1292
BmcAI	AGTACT	1 cut(s)	871
Bme1390I	CCNGG	2 cut(s)	1374, 3042
Bme18I	GGWCC	5 cut(s)	81, 692, 1370, 2215, 3688
BmeRI	GACNNNNNGTC	1 cut(s)	271
BmiI	GGNNCC	6 cut(s)	163, 225, 694, 1754, 1755, 3689
BmrFI	CCNGG	2 cut(s)	1374, 3042
BmsI	GCATC	5 cut(s)	52, 1541, 2708, 3459, 3761
BmtI	GCTAGC	1 cut(s)	3560
BoxI	GACNNNNGTC	1 cut(s)	3196
BpiI	GAAGAC	2 cut(s)	219, 3662
Bpu10I	CCTNAGC	1 cut(s)	1791
Bpu14I	TTCGAA	1 cut(s)	337
BpuEI	CTTGAG	7 cut(s)	1298, 2306, 2726, 2742, 2771, 3685, 3743
BsaBI	GATNNNNATC	2 cut(s)	1776, 3527
BsaHI	GRCGYC	1 cut(s)	162
BsaI	GGTCTC	2 cut(s)	641, 2768
BsaJI	CCNNGG	7 cut(s)	989, 1071, 1292, 2240, 3244, 3691, 3773
BsaWI	WCCGGW	1 cut(s)	174
BsaXI	ACNNNNNCTCC	6 cut(s)	97, 127, 1340, 1370, 3156, 3186
Bsc4I	CCNNNNNNNGG	4 cut(s)	689, 797, 1490, 1600
Bse118I	RCCGGY	2 cut(s)	164, 174
Bse21I	CCTNAGG	2 cut(s)	924, 3393
Bse3DI	GCAATG	4 cut(s)	694, 2473, 3481, 3708
Bse8I	GATNNNNATC	2 cut(s)	1776, 3527
BseBI	CCWGG	2 cut(s)	1374, 3042
BseDI	CCNNGG	7 cut(s)	989, 1071, 1292, 2240, 3244, 3691, 3773
BseGI	GGATG	9 cut(s)	1432, 1777, 1954, 2038, 2092, 2566, 2716, 2953, 3659
BseJI	GATNNNNATC	2 cut(s)	1776, 3527
BseLI	CCNNNNNNNGG	4 cut(s)	689, 797, 1490, 1600
BseMI	GCAATG	4 cut(s)	694, 2473, 3481, 3708
BseRI	GAGGAG	6 cut(s)	109, 659, 1781, 1808, 1970, 2837
BseSI	GKGCMC	2 cut(s)	1757, 2799
BseYI	CCCAGC	2 cut(s)	602, 759
BsgI	GTGCAG	1 cut(s)	2447
BshNI	GGYRCC	2 cut(s)	161, 223
BshTI	ACCGGT	1 cut(s)	174
BsiHKAI	GWGCWC	2 cut(s)	2799, 3391
BsiSI	CCGG	2 cut(s)	165, 175
BslFI	GGGAC	5 cut(s)	449, 619, 1616, 3420, 3701
BslI	CCNNNNNNNGG	4 cut(s)	689, 797, 1490, 1600
BsmAI	GTCTC	6 cut(s)	98, 641, 1359, 1639, 2768, 3338
BsmBI	CGTCTC	2 cut(s)	1359, 3338
BsmFI	GGGAC	5 cut(s)	449, 619, 1616, 3420, 3701
BsmI	GAATGC	2 cut(s)	626, 2903
Bso31I	GGTCTC	2 cut(s)	641, 2768
Bsp119I	TTCGAA	1 cut(s)	337
Bsp120I	GGGCCC	1 cut(s)	1753
Bsp1286I	GDGCHC	3 cut(s)	1757, 2799, 3391
BspACI	CCGC	5 cut(s)	159, 301, 696, 2720, 2869
BspHI	TCATGA	1 cut(s)	2926
BspLI	GGNNCC	6 cut(s)	163, 225, 694, 1754, 1755, 3689
BspMAI	CTGCAG	1 cut(s)	3198
BspMI	ACCTGC	2 cut(s)	1165, 3508
BspOI	GCTAGC	1 cut(s)	3560
BspQI	GCTCTTC	3 cut(s)	339, 1186, 1410
BspT104I	TTCGAA	1 cut(s)	337
BspT107I	GGYRCC	2 cut(s)	161, 223
BspTI	CTTAAG	2 cut(s)	944, 1204
BspTNI	GGTCTC	2 cut(s)	641, 2768
BsrDI	GCAATG	4 cut(s)	694, 2473, 3481, 3708
BsrFI	RCCGGY	2 cut(s)	164, 174
BssAI	RCCGGY	2 cut(s)	164, 174
BssECI	CCNNGG	7 cut(s)	989, 1071, 1292, 2240, 3244, 3691, 3773
BssNI	GRCGYC	1 cut(s)	162
BssT1I	CCWWGG	5 cut(s)	989, 1071, 1292, 3244, 3691
Bst2UI	CCWGG	2 cut(s)	1374, 3042
Bst4CI	ACNGT	7 cut(s)	208, 652, 1057, 1561, 1941, 2335, 3299
BstACI	GRCGYC	1 cut(s)	162
BstAFI	CTTAAG	2 cut(s)	944, 1204
BstAPI	GCANNNNNTGC	1 cut(s)	2425
BstBI	TTCGAA	1 cut(s)	337
BstC8I	GCNNGC	8 cut(s)	166, 2430, 2434, 2508, 2643, 2866, 3554, 3558
BstDSI	CCRYGG	2 cut(s)	2240, 3773
BstENI	CCTNNNNNAGG	1 cut(s)	1488
BstF5I	GGATG	9 cut(s)	1432, 1777, 1954, 2038, 2092, 2566, 2716, 2953, 3659
BstH2I	RGCGCY	3 cut(s)	165, 3227, 3745
BstHHI	GCGC	3 cut(s)	164, 3226, 3744
BstMAI	GTCTC	6 cut(s)	98, 641, 1359, 1639, 2768, 3338
BstNI	CCWGG	2 cut(s)	1374, 3042
BstNSI	RCATGY	3 cut(s)	2645, 3556, 3604
BstPAI	GACNNNNGTC	1 cut(s)	3196
BstSCI	CCNGG	2 cut(s)	1372, 3040
BstSFI	CTRYAG	3 cut(s)	1485, 3194, 3295
BstSLI	GKGCMC	2 cut(s)	1757, 2799
BstV2I	GAAGAC	2 cut(s)	219, 3662
BstX2I	RGATCY	5 cut(s)	467, 1738, 2197, 2585, 3377
BstYI	RGATCY	5 cut(s)	467, 1738, 2197, 2585, 3377
Bsu36I	CCTNAGG	2 cut(s)	924, 3393
BsuI	GTATCC	1 cut(s)	1631
BtgI	CCRYGG	2 cut(s)	2240, 3773
BtsCI	GGATG	9 cut(s)	1432, 1777, 1954, 2038, 2092, 2566, 2716, 2953, 3659
BtsI	GCAGTG	1 cut(s)	2161
BtsIMutI	CAGTG	4 cut(s)	1887, 2161, 2405, 3218
BveI	ACCTGC	2 cut(s)	1165, 3508
Cac8I	GCNNGC	8 cut(s)	166, 2430, 2434, 2508, 2643, 2866, 3554, 3558
CaiI	CAGNNNCTG	2 cut(s)	1886, 1982
CciI	TCATGA	1 cut(s)	2926
CfoI	GCGC	3 cut(s)	164, 3226, 3744
Cfr10I	RCCGGY	2 cut(s)	164, 174
CseI	GACGC	2 cut(s)	1538, 3767
Csp6I	GTAC	8 cut(s)	870, 954, 1430, 2162, 3052, 3070, 3468, 3479
CspAI	ACCGGT	1 cut(s)	174
CspCI	CAANNNNNGTGG	2 cut(s)	2103, 2138
CviQI	GTAC	8 cut(s)	870, 954, 1430, 2162, 3052, 3070, 3468, 3479
DinI	GGCGCC	1 cut(s)	163
DriI	GACNNNNNGTC	1 cut(s)	271
EaeI	YGGCCR	4 cut(s)	261, 992, 2866, 3456
Eam1105I	GACNNNNNGTC	1 cut(s)	271
Eco130I	CCWWGG	5 cut(s)	989, 1071, 1292, 3244, 3691
Eco24I	GRGCYC	1 cut(s)	1757
Eco31I	GGTCTC	2 cut(s)	641, 2768
Eco32I	GATATC	1 cut(s)	2149
Eco47I	GGWCC	5 cut(s)	81, 692, 1370, 2215, 3688
Eco47III	AGCGCT	1 cut(s)	3225
Eco57I	CTGAAG	8 cut(s)	362, 804, 1008, 1440, 1842, 2502, 3212, 3785
Eco81I	CCTNAGG	2 cut(s)	924, 3393
EcoNI	CCTNNNNNAGG	1 cut(s)	1488
EcoO109I	RGGNCCY	1 cut(s)	1753
EcoRI	GAATTC	2 cut(s)	890, 2851
EcoRII	CCWGG	2 cut(s)	1372, 3040
EcoRV	GATATC	1 cut(s)	2149
EcoT14I	CCWWGG	5 cut(s)	989, 1071, 1292, 3244, 3691
EcoT38I	GRGCYC	1 cut(s)	1757
EgeI	GGCGCC	1 cut(s)	163
EheI	GGCGCC	1 cut(s)	163
ErhI	CCWWGG	5 cut(s)	989, 1071, 1292, 3244, 3691
Esp3I	CGTCTC	2 cut(s)	1359, 3338
FalI	AAGNNNNNCTT	4 cut(s)	1944, 1976, 3647, 3679
FaqI	GGGAC	5 cut(s)	449, 619, 1616, 3420, 3701
FauI	CCCGC	1 cut(s)	703
FauNDI	CATATG	1 cut(s)	3415
FbaI	TGATCA	3 cut(s)	1777, 2989, 3544
FblI	GTMKAC	1 cut(s)	3174
FokI	GGATG	9 cut(s)	1439, 1784, 1961, 2045, 2099, 2573, 2723, 2960, 3646
FriOI	GRGCYC	1 cut(s)	1757
GlaI	GCGC	3 cut(s)	163, 3225, 3743
GsaI	CCCAGC	2 cut(s)	606, 763
HaeII	RGCGCY	3 cut(s)	165, 3227, 3745
HapII	CCGG	2 cut(s)	165, 175
HgaI	GACGC	2 cut(s)	1538, 3767
HhaI	GCGC	3 cut(s)	164, 3226, 3744
Hin1I	GRCGYC	1 cut(s)	162
Hin6I	GCGC	3 cut(s)	162, 3224, 3742
HinP1I	GCGC	3 cut(s)	162, 3224, 3742
HincII	GTYRAC	3 cut(s)	280, 1086, 1146
HindII	GTYRAC	3 cut(s)	280, 1086, 1146
HindIII	AAGCTT	3 cut(s)	66, 383, 3011
HpaI	GTTAAC	1 cut(s)	1086
HpaII	CCGG	2 cut(s)	165, 175
Hpy166II	GTNNAC	9 cut(s)	280, 920, 1086, 1146, 2312, 2444, 2797, 3113, 3175
Hpy8I	GTNNAC	9 cut(s)	280, 920, 1086, 1146, 2312, 2444, 2797, 3113, 3175
Hpy99I	CGWCG	1 cut(s)	421
HpyAV	CCTTC	9 cut(s)	386, 1032, 1118, 2052, 2186, 2284, 2375, 2788, 3756
HpyCH4III	ACNGT	7 cut(s)	208, 652, 1057, 1561, 1941, 2335, 3299
HpyCH4IV	ACGT	2 cut(s)	282, 3024
HpySE526I	ACGT	2 cut(s)	282, 3024
Hsp92I	GRCGYC	1 cut(s)	162
HspAI	GCGC	3 cut(s)	162, 3224, 3742
KasI	GGCGCC	1 cut(s)	161
KroI	GCCGGC	1 cut(s)	164
KroNI	GCCGGC	1 cut(s)	166
Ksp22I	TGATCA	3 cut(s)	1777, 2989, 3544
KspAI	GTTAAC	1 cut(s)	1086
LguI	GCTCTTC	3 cut(s)	339, 1186, 1410
LmnI	GCTCC	4 cut(s)	1171, 1957, 3365, 3394
LweI	GCATC	5 cut(s)	52, 1541, 2708, 3459, 3761
MaeII	ACGT	2 cut(s)	282, 3024
MfeI	CAATTG	1 cut(s)	2420
MflI	RGATCY	5 cut(s)	467, 1738, 2197, 2585, 3377
MhlI	GDGCHC	3 cut(s)	1757, 2799, 3391
MlsI	TGGCCA	1 cut(s)	994
MluNI	TGGCCA	1 cut(s)	994
Mly113I	GGCGCC	1 cut(s)	162
MlyI	GAGTC	6 cut(s)	260, 1598, 2252, 2543, 2593, 3167
MmeI	TCCRAC	2 cut(s)	399, 1615
Mox20I	TGGCCA	1 cut(s)	994
MroNI	GCCGGC	1 cut(s)	164
MroXI	GAANNNNTTC	6 cut(s)	22, 371, 2208, 2551, 3225, 3532
MscI	TGGCCA	1 cut(s)	994
MslI	CAYNNNNRTG	1 cut(s)	1728
Msp20I	TGGCCA	1 cut(s)	994
MspA1I	CMGCKG	2 cut(s)	159, 2900
MspCI	CTTAAG	2 cut(s)	944, 1204
MspI	CCGG	2 cut(s)	165, 175
MspR9I	CCNGG	2 cut(s)	1374, 3042
MunI	CAATTG	1 cut(s)	2420
Mva1269I	GAATGC	2 cut(s)	626, 2903
MvaI	CCWGG	2 cut(s)	1374, 3042
NaeI	GCCGGC	1 cut(s)	166
NarI	GGCGCC	1 cut(s)	162
NdeI	CATATG	1 cut(s)	3415
NgoMIV	GCCGGC	1 cut(s)	164
NheI	GCTAGC	1 cut(s)	3556
NlaIV	GGNNCC	6 cut(s)	163, 225, 694, 1754, 1755, 3689
NmuCI	GTSAC	9 cut(s)	177, 1212, 1700, 1882, 1999, 2878, 3044, 3260, 3438
NspI	RCATGY	3 cut(s)	2645, 3556, 3604
NspV	TTCGAA	1 cut(s)	337
PaeI	GCATGC	2 cut(s)	2645, 3556
PagI	TCATGA	1 cut(s)	2926
PaqCI	CACCTGC	1 cut(s)	3508
PciSI	GCTCTTC	3 cut(s)	339, 1186, 1410
PctI	GAATGC	2 cut(s)	626, 2903
PdiI	GCCGGC	1 cut(s)	166
PdmI	GAANNNNTTC	6 cut(s)	22, 371, 2208, 2551, 3225, 3532
PinAI	ACCGGT	1 cut(s)	174
PleI	GAGTC	6 cut(s)	260, 1598, 2252, 2542, 2593, 3166
PluTI	GGCGCC	1 cut(s)	165
PpsI	GAGTC	6 cut(s)	260, 1598, 2252, 2542, 2593, 3166
PshAI	GACNNNNGTC	1 cut(s)	3196
PsiI	TTATAA	2 cut(s)	1853, 2966
Psp6I	CCWGG	2 cut(s)	1372, 3040
PspFI	CCCAGC	2 cut(s)	602, 759
PspGI	CCWGG	2 cut(s)	1372, 3040
PspN4I	GGNNCC	6 cut(s)	163, 225, 694, 1754, 1755, 3689
PspOMI	GGGCCC	1 cut(s)	1753
PstI	CTGCAG	1 cut(s)	3198
PstNI	CAGNNNCTG	2 cut(s)	1886, 1982
PsuI	RGATCY	5 cut(s)	467, 1738, 2197, 2585, 3377
PvuII	CAGCTG	1 cut(s)	2900
RsaI	GTAC	8 cut(s)	871, 955, 1431, 2163, 3053, 3071, 3469, 3480
RsaNI	GTAC	8 cut(s)	870, 954, 1430, 2162, 3052, 3070, 3468, 3479
RseI	CAYNNNNRTG	1 cut(s)	1728
SapI	GCTCTTC	3 cut(s)	339, 1186, 1410
ScaI	AGTACT	1 cut(s)	871
SchI	GAGTC	6 cut(s)	260, 1598, 2252, 2543, 2593, 3167
ScrFI	CCNGG	2 cut(s)	1374, 3042
SduI	GDGCHC	3 cut(s)	1757, 2799, 3391
SfaNI	GCATC	5 cut(s)	52, 1541, 2708, 3459, 3761
SfcI	CTRYAG	3 cut(s)	1485, 3194, 3295
SfoI	GGCGCC	1 cut(s)	163
SfuI	TTCGAA	1 cut(s)	337
SinI	GGWCC	5 cut(s)	81, 692, 1370, 2215, 3688
SmiMI	CAYNNNNRTG	1 cut(s)	1728
SmlI	CTYRAG	9 cut(s)	944, 1204, 1313, 2285, 2705, 2757, 2786, 3664, 3722
SmoI	CTYRAG	9 cut(s)	944, 1204, 1313, 2285, 2705, 2757, 2786, 3664, 3722
SpeI	ACTAGT	1 cut(s)	2306
SphI	GCATGC	2 cut(s)	2645, 3556
SsiI	CCGC	5 cut(s)	159, 301, 696, 2720, 2869
SspDI	GGCGCC	1 cut(s)	161
StyD4I	CCNGG	2 cut(s)	1372, 3040
StyI	CCWWGG	5 cut(s)	989, 1071, 1292, 3244, 3691
TaaI	ACNGT	7 cut(s)	208, 652, 1057, 1561, 1941, 2335, 3299
TaiI	ACGT	2 cut(s)	285, 3027
TaqI	TCGA	6 cut(s)	95, 337, 1002, 2456, 2597, 3088
TatI	WGTACW	3 cut(s)	869, 3467, 3478
TauI	GCSGC	3 cut(s)	162, 2723, 2871
TscAI	CASTG	4 cut(s)	1887, 2161, 2412, 3218
TseFI	GTSAC	9 cut(s)	177, 1212, 1700, 1882, 1999, 2878, 3044, 3260, 3438
Tsp45I	GTSAC	9 cut(s)	177, 1212, 1700, 1882, 1999, 2878, 3044, 3260, 3438
TspGWI	ACGGA	5 cut(s)	265, 1383, 2257, 3362, 3628
TspRI	CASTG	4 cut(s)	1887, 2161, 2412, 3218
Vha464I	CTTAAG	2 cut(s)	944, 1204
VneI	GTGCAC	1 cut(s)	2795
VpaK11BI	GGWCC	5 cut(s)	81, 692, 1370, 2215, 3688
XagI	CCTNNNNNAGG	1 cut(s)	1488
XapI	RAATTY	5 cut(s)	890, 2370, 2833, 2851, 3137
XbaI	TCTAGA	2 cut(s)	2200, 3217
XceI	RCATGY	3 cut(s)	2645, 3556, 3604
XmaJI	CCTAGG	1 cut(s)	1292
XmiI	GTMKAC	1 cut(s)	3174
XmnI	GAANNNNTTC	6 cut(s)	22, 371, 2208, 2551, 3225, 3532
ZrmI	AGTACT	1 cut(s)	871

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.