Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0006728

Physical Location & Seq

Genomic Coordinates Forward (+)

32073594 .. 32080729

Gene Structure 7136 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1242100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3576 bp

ATGGAAGGAAGGAGTTTTAGGAGGCTGAAAATGGCGGACTCAAACTCTGTTGACGTTATTCTGGACTTTCTGCGGAAGAATAGGTTTTCAAGAGCAGAGGCAGCTCTGCGAAGTGAGCTTGGTAATCGCTCTGATTTGAATGGTTTCCTTCAGAAGCTTACTCTTGAAGAGAAGGATTCAGGTCGTTTGTTGGAAGCTGAAAACGGGGACAAGCTAGTGGTGGAAAATCAAGGCTTGGGATCTCGGAATGGTGGTGAAGTCTATAAGGAACTTATAGTGAAAGAGATAGAGTGTGGAACTGGTAGAAATGGGTCTGAAAGCAAATTGAAAAATGCTGCTTCTATTGGGGAGCGGAATAAGTCTATTGAGGTGGCTGGGACAAATCACAAGAGCTTTGCATTCTCTAAAGGTTTAGAGGACACCGTGCTTGATTTATATTCTTGGAAGTCCAGTCCGAGCAATGGACCTGCAGAACCTTATCAAAATGATGGTGGTGGTATTATCGCTAACTTCCCAGAGCCTCAGATATCCCAACAATCAAAGAATCATACAACTGAAGTTCCTGATTCAGGTAAAGCCATTGTGAAATATGGAGAAGAGATTAGCTTTTCTGGTGAAAAGAAAACCTCTTGGTCTGGAAGTACTAGCAAAGCTAGTGTCGAATTCAAGTGTGACAGAACCCAAACAAGCGAACCTAAGGAACTTGAACAACTTAAGACGAGTACCATGGTTTTCAAAGAAAATGCAGCAGGGAATCCTTGGTCAAGAATCGAAGAACCAACGAATCCACCTTCAGAGATGTGGAAAGATTGTTCCGTCAAGACCGCGTTTCCTTTCTCCAAGGGTGATGTGTCAACGAGTTATGATAGTGCTCCTGGTTCTGACAAAAAAGAAGGAAAGAGAAAAACTGAGTTGGCTGATATTAGGGCAACAGTAAAGGAGCAGGTTGATGAGGTGGGAAGAGCTTTATACTTAAGCCAGTCACAAGGGATTTCTGAGCAAAAGACCATTAGTAATTTAGTTTTTCCTATTTTGTCTGAGAATCAAAAGGAAGAGTTCCCTAGGCTGCCACCCGTCAAACTCAAGTCAGAGGACAAACCAAATGTTAATTGGGAGGAAAAGTTTGAAAGAGACGGACCAGGCGCAAAACTCAGCATTGCTGACAATGCTCATCTTATTGGGTCATATTTGGATGTACCTATTGGACAAGAAATCAACTCTTCAGATTATGGTTCAGGTGGAAAAAGGCCTGTAGGAGGTAGTTGGCTGTCTGTAAGTCAGGGGATTGCAGAGGACGCATCTGATCTGGTTTCTGGTTTTGCTACTGTTGGTGATGGTTTGAGTGAATCGATTGACTACCCATATTGGGACTCTGATGAATATGATGATGATGATGATGTCGGATACATGAGACAACCGATTGAAGATGAGGCGTGGTTTTTGGCCCATGAAATTGATTACCCTAGTGACAATGAAAAAGGAACAGGTCATGGCAGTGTTCCAGATCCACAGGAAAGGGGTCCAACCAAAGATGAGGATGATGATCAATCTTTTGCTGAGGAGGATTCTTATTTCTCTGGTGAGCGGTGCTTCCAAGCGAAAAATGTTGAACCGATTATAAGTTCAGATGATCCTATAGGATTATCAGTGACTGAATTGTATGGGAGAACTGATGATAATGGTTTAATAGCTCAATATGATGGACAATTGATGGATGAAGAGGAGCTTAATTTAATGCGTTCAGAACCTGTCTGGCAGGGCTTTGTCACTCAGACCAATGAACTCATCATGTTAGGGAATGGGAAAGTTGTGAATGAGAGTGGAAGGCCACGTTTGGAGGAGGTTTGTGTGGATGACGACCAGCTTGGTTCAGTAAGATCAATAGGTGTGGGAATCAATAGCGATGCTGCTGATATCGGCAGCGAAGTGCGGGAAAGTTTGATTGGTGGCAGTAGTGAAGGGGATCTAGAATACTTTCGGGACCATGATGTAGGAATTGGTGGGCCACAGAAGAATCATCAGGACTCAGATAAGAAACGTATTGATAGACTTGAGAGGGATAAGAAGAAAACTAGTAAACATGAGGCAAGTAAATATATAGTTGAGAATGATAATGGTGTATTTAGACAGAAGAAAAATCATTCAGAAGGGGGATTTTCATTCCCGCCGCCACTGAGAGATGGACAATTGGTGCAGGCAAGCTCTAGTAAACCGTTATGGTCAAACAACTTCAATGCTGTTGTCACTGAAGAACCTGATGATGATAACATGCTTGCTTCATGGAGGGAGAAAAGTAACGAGTCCTCACCTAGAATGAGTTCTAGGGATGAAAGAAATGCTAATGCTGTTAGATCCACAAACTCTACTCCCTCAACGCTCTCTAATTATGCTTATGCTGAAAGAGAGCACGCCAAGCAAGAAGAAGATGAGAAAATTGCTGCTGTTAGGGAAGAAGATACGGGGGCATCACTTGAGGATGAAGAGGCGGCTGCTGTGCAAGAACAAGTAAGGCAAATAAAGGCTCAAGAGGAAGAGTTTGAGACCTTCAACCTCAAGATTGTGCACAGGAAAAACAGAACTGGCTTTGAGGAGGACAAAAATTTTCAAGTTGTTTTGAATTCTGTATTAGCTGGCCGCTATCATGTTACTGAATATCTTGGATCAGCAGCATTCAGCAAAGCCATACAAGCTCATGATCTACATACAGGCATGGATGTGTGTGTGAAAATTATAAAGAACAACAAAGACTTCTTTGATCAGAGCCTTGATGAGATAAAGCTTCTCAAATACGTCAATAAGCATGATCCTGGTGACAAGTATCACATTCTTCGGTTGTACGATTACTTCTACTATCGAGAACATTTGTTAATAGTATGTGAACTTCTCAAAGCAAACTTGTATGAATTTCATAAATTTAATCGGGAGTCAGGAGGGGAGGTCTACTTCACAATGCCACGACTGCAGTCAATTACGATTCAGTGTCTAGAGGCGCTTCAGTTTTTGCATGGCCTTGGCATGATACACTGTGACTTGAAGCCTGAGAATATACTGGTCAAAAGCTACAGTAGATGTGAGGTCAAAGTCATTGATCTTGGGAGTAGTTGCTTTGAGACGGATCATCTCTGCTCCTACGTTCAGTCCAGATCCTATCGTGCTCCTGAGGTTATCTTGGGACTTCCATATGATAAGAAGATAGATATATGGTCGCTTGGCTGCATCTTGGCTGAACTTTGTACTGGCAATGTACTCTTCCAGAATGATTCCCCTGCAACTTTACTTGCACGGGTGATGGGAATCATTTCCCCTATTGATCAAGGCATGCTAGCCAAAGGACGTGATACATACAAATACTTCACAAAAAATCACATGCTTTATGAACGGAATCAGGAAACAAACAGACTGGAATACCTGATACCAAAGAAGACATCCTTGAGGCACAGGCTTCCAATGGGGGACCAAGGCTTCATAGACTTCGTTGCTCATCTGCTTGAGATAAACCCGAAGAAGCGCCCTTCTGCATCTGAAGCTCTGAAGCACCCGTGGCTATCATATCCTTACGAACCCATATCATCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

1192

Amino Acids

133.51

Weight (kDa)

4.98

Isoelectric Point (pI)

43.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	880 - 1182	4.3e-50	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	882 - 1086	2.1e-17	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006728)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G73450 AT1G73460 AT1G73460 AT3G17750
fragaria_vesca	FvH4_5g17930 FvH4_5g17930 FvH4_5g17930
malus_domestica	MD06G1234900.v1.1 MD14G1242100.v1.1
prunus_persica	Prupe.5G239700_v2.0.a1 Prupe.5G239700_v2.0.a1 Prupe.5G239700_v2.0.a1 Prupe.5G239700_v2.0.a1
pyrus_communis	pycom06g20990 pycom14g20350
rosa_chinensis	RchiOBHm_Chr7g0177671
rosa_laevigata	RLG00000005515
rosa_multiflora	Rmu_co8343931.1_g000001 Rmu_ssc0000236.1_g000003
rosa_roxburghii	Rroxscaffold_3G00275670
rosa_rugosa	Rorug06G0408400
rosa_samantha	Rh7AG007900 Rh7BG007800 Rh7CG008300 Rh7DG008000
rosa_wichuraiana	Rw7G000660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1619, 2732
Acc36I	ACCTGC	2 cut(s)	475, 934
AccBSI	CCGCTC	2 cut(s)	352, 1585
AccI	GTMKAC	1 cut(s)	2940
AccII	CGCG	1 cut(s)	827
AclWI	GGATC	9 cut(s)	247, 1499, 1625, 1971, 2346, 2668, 2798, 3123, 3138
AcoI	YGGCCR	1 cut(s)	2632
AcsI	RAATTY	5 cut(s)	662, 2599, 2617, 2903, 2912
AcuI	CTGAAG	8 cut(s)	134, 576, 777, 1206, 2268, 2978, 3543, 3551
AfaI	GTAC	6 cut(s)	643, 724, 1197, 2837, 3235, 3246
AfiI	CCNNNNNNNGG	4 cut(s)	461, 569, 1256, 1366
AflII	CTTAAG	2 cut(s)	713, 973
AhlI	ACTAGT	1 cut(s)	2072
AjiI	CACGTC	1 cut(s)	3335
AjnI	CCWGG	3 cut(s)	874, 1138, 2806
AjuI	GAANNNNNNNTTGG	2 cut(s)	1926, 1958
AloI	GAACNNNNNNTCC	2 cut(s)	796, 828
Alw21I	GWGCWC	4 cut(s)	874, 2409, 2565, 3157
Alw26I	GTCTC	4 cut(s)	1125, 1405, 2534, 3104
Alw44I	GTGCAC	1 cut(s)	2561
AlwI	GGATC	9 cut(s)	247, 1499, 1625, 1971, 2346, 2668, 2798, 3123, 3138
AlwNI	CAGNNNCTG	2 cut(s)	1652, 1748
AoxI	GGCC	6 cut(s)	1247, 1443, 1826, 2003, 2632, 3007
ApaLI	GTGCAC	1 cut(s)	2561
ApoI	RAATTY	5 cut(s)	662, 2599, 2617, 2903, 2912
ArsI	GACNNNNNNTTYG	2 cut(s)	642, 674
Asp700I	GAANNNNTTC	4 cut(s)	143, 1974, 2317, 3298
AspA2I	CCTAGG	1 cut(s)	1061
AspLEI	GCGC	3 cut(s)	1145, 2992, 3510
AspS9I	GGNCC	7 cut(s)	464, 1136, 1444, 1520, 1981, 2003, 3454
AsuHPI	GGTGA	8 cut(s)	266, 626, 857, 1343, 1592, 2298, 2822, 3298
AsuNHI	GCTAGC	1 cut(s)	3322
AvaII	GGWCC	5 cut(s)	464, 1136, 1520, 1981, 3454
AvrII	CCTAGG	1 cut(s)	1061
AxyI	CCTNAGG	2 cut(s)	696, 3159
BaeGI	GKGCMC	1 cut(s)	2565
BarI	GAAGNNNNNNTAC	4 cut(s)	2828, 2860, 2865, 2897
BbsI	GAAGAC	1 cut(s)	3428
Bbv12I	GWGCWC	4 cut(s)	874, 2409, 2565, 3157
BbvCI	CCTCAGC	1 cut(s)	1557
BccI	CCATC	6 cut(s)	482, 1328, 1694, 1705, 2174, 3283
BciT130I	CCWGG	3 cut(s)	876, 1140, 2808
BciVI	GTATCC	1 cut(s)	1397
BclI	TGATCA	3 cut(s)	1543, 2755, 3310
BcoDI	GTCTC	4 cut(s)	1125, 1405, 2534, 3104
BcuI	ACTAGT	1 cut(s)	2072
BfmI	CTRYAG	5 cut(s)	468, 1251, 1635, 2960, 3061
BfoI	RGCGCY	2 cut(s)	2993, 3511
BfrI	CTTAAG	2 cut(s)	713, 973
BfuAI	ACCTGC	2 cut(s)	475, 934
BfuI	GTATCC	1 cut(s)	1397
BlnI	CCTAGG	1 cut(s)	1061
BmcAI	AGTACT	1 cut(s)	643
Bme1390I	CCNGG	3 cut(s)	876, 1140, 2808
Bme18I	GGWCC	5 cut(s)	464, 1136, 1520, 1981, 3454
BmgBI	CACGTC	1 cut(s)	3335
BmgT120I	GGNCC	7 cut(s)	464, 1136, 1444, 1520, 1981, 2003, 3454
BmiI	GGNNCC	3 cut(s)	1521, 1982, 3455
BmrFI	CCNGG	3 cut(s)	876, 1140, 2808
BmsI	GCATC	5 cut(s)	1307, 1894, 2474, 3225, 3527
BmtI	GCTAGC	1 cut(s)	3326
BoxI	GACNNNNGTC	1 cut(s)	2962
BpiI	GAAGAC	1 cut(s)	3428
Bpu10I	CCTNAGC	1 cut(s)	1557
BpuEI	CTTGAG	7 cut(s)	1067, 2072, 2492, 2508, 2537, 3451, 3509
Bsa29I	ATCGAT	1 cut(s)	1349
BsaBI	GATNNNNATC	2 cut(s)	1542, 3293
BsaI	GGTCTC	1 cut(s)	2534
BsaJI	CCNNGG	7 cut(s)	726, 758, 840, 1061, 3010, 3457, 3539
BsaXI	ACNNNNNCTCC	4 cut(s)	1106, 1136, 2922, 2952
Bsc4I	CCNNNNNNNGG	4 cut(s)	461, 569, 1256, 1366
Bse1I	ACTGG	7 cut(s)	304, 450, 979, 2584, 3054, 3241, 3405
Bse21I	CCTNAGG	2 cut(s)	696, 3159
Bse3DI	GCAATG	3 cut(s)	466, 1155, 3247
Bse8I	GATNNNNATC	2 cut(s)	1542, 3293
BseBI	CCWGG	3 cut(s)	876, 1140, 2808
BseCI	ATCGAT	1 cut(s)	1349
BseDI	CCNNGG	7 cut(s)	726, 758, 840, 1061, 3010, 3457, 3539
BseGI	GGATG	8 cut(s)	1198, 1543, 1720, 1858, 2332, 2482, 2719, 3425
BseJI	GATNNNNATC	2 cut(s)	1542, 3293
BseLI	CCNNNNNNNGG	4 cut(s)	461, 569, 1256, 1366
BseMI	GCAATG	3 cut(s)	466, 1155, 3247
BseNI	ACTGG	7 cut(s)	304, 450, 979, 2584, 3054, 3241, 3405
BseRI	GAGGAG	4 cut(s)	1574, 1736, 1853, 2603
BseSI	GKGCMC	1 cut(s)	2565
BseYI	CCCAGC	1 cut(s)	374
BsgI	GTGCAG	1 cut(s)	2213
Bsh1236I	CGCG	1 cut(s)	827
BshFI	GGCC	6 cut(s)	1249, 1445, 1828, 2005, 2634, 3009
BshVI	ATCGAT	1 cut(s)	1349
BsiHKAI	GWGCWC	4 cut(s)	874, 2409, 2565, 3157
BslFI	GGGAC	6 cut(s)	221, 391, 1382, 1994, 3186, 3467
BslI	CCNNNNNNNGG	4 cut(s)	461, 569, 1256, 1366
BsmAI	GTCTC	4 cut(s)	1125, 1405, 2534, 3104
BsmBI	CGTCTC	2 cut(s)	1125, 3104
BsmFI	GGGAC	6 cut(s)	221, 391, 1382, 1994, 3186, 3467
BsmI	GAATGC	2 cut(s)	398, 2669
BsnI	GGCC	6 cut(s)	1249, 1445, 1828, 2005, 2634, 3009
Bso31I	GGTCTC	1 cut(s)	2534
Bsp1286I	GDGCHC	4 cut(s)	874, 2409, 2565, 3157
Bsp19I	CCATGG	1 cut(s)	726
BspANI	GGCC	6 cut(s)	1249, 1445, 1828, 2005, 2634, 3009
BspDI	ATCGAT	1 cut(s)	1349
BspFNI	CGCG	1 cut(s)	827
BspHI	TCATGA	1 cut(s)	2692
BspLI	GGNNCC	3 cut(s)	1521, 1982, 3455
BspMAI	CTGCAG	2 cut(s)	472, 2964
BspMI	ACCTGC	2 cut(s)	475, 934
BspOI	GCTAGC	1 cut(s)	3326
BspPI	GGATC	9 cut(s)	247, 1499, 1625, 1971, 2346, 2668, 2798, 3123, 3138
BspQI	GCTCTTC	1 cut(s)	955
BspTI	CTTAAG	2 cut(s)	713, 973
BspTNI	GGTCTC	1 cut(s)	2534
BsrBI	CCGCTC	2 cut(s)	352, 1585
BsrDI	GCAATG	3 cut(s)	466, 1155, 3247
BsrI	ACTGG	7 cut(s)	304, 450, 979, 2584, 3054, 3241, 3405
BssECI	CCNNGG	7 cut(s)	726, 758, 840, 1061, 3010, 3457, 3539
BssT1I	CCWWGG	6 cut(s)	726, 758, 840, 1061, 3010, 3457
Bst2UI	CCWGG	3 cut(s)	876, 1140, 2808
Bst4CI	ACNGT	6 cut(s)	424, 934, 1327, 2214, 3026, 3065
Bst6I	CTCTTC	9 cut(s)	162, 591, 955, 1047, 1225, 1713, 2475, 2526, 3254
BstAFI	CTTAAG	2 cut(s)	713, 973
BstC8I	GCNNGC	7 cut(s)	2196, 2200, 2274, 2409, 2632, 3320, 3324
BstDSI	CCRYGG	2 cut(s)	726, 3539
BstENI	CCTNNNNNAGG	2 cut(s)	567, 1254
BstF5I	GGATG	8 cut(s)	1198, 1543, 1720, 1858, 2332, 2482, 2719, 3425
BstFNI	CGCG	1 cut(s)	827
BstH2I	RGCGCY	2 cut(s)	2993, 3511
BstHHI	GCGC	3 cut(s)	1145, 2992, 3510
BstMAI	GTCTC	4 cut(s)	1125, 1405, 2534, 3104
BstMWI	GCNNNNNNNGC	9 cut(s)	101, 115, 1166, 1295, 2413, 2687, 2959, 3524, 3541
BstNI	CCWGG	3 cut(s)	876, 1140, 2808
BstNSI	RCATGY	3 cut(s)	2272, 3322, 3370
BstPAI	GACNNNNGTC	1 cut(s)	2962
BstSCI	CCNGG	3 cut(s)	874, 1138, 2806
BstSFI	CTRYAG	5 cut(s)	468, 1251, 1635, 2960, 3061
BstSLI	GKGCMC	1 cut(s)	2565
BstUI	CGCG	1 cut(s)	827
BstV2I	GAAGAC	1 cut(s)	3428
BstX2I	RGATCY	5 cut(s)	239, 1504, 1963, 2351, 3143
BstYI	RGATCY	5 cut(s)	239, 1504, 1963, 2351, 3143
Bsu15I	ATCGAT	1 cut(s)	1349
Bsu36I	CCTNAGG	2 cut(s)	696, 3159
BsuI	GTATCC	1 cut(s)	1397
BsuRI	GGCC	6 cut(s)	1249, 1445, 1828, 2005, 2634, 3009
BsuTUI	ATCGAT	1 cut(s)	1349
BtgI	CCRYGG	2 cut(s)	726, 3539
BtgZI	GCGATG	1 cut(s)	1917
BtrI	CACGTC	1 cut(s)	3335
BtsCI	GGATG	8 cut(s)	1198, 1543, 1720, 1858, 2332, 2482, 2719, 3425
BtsI	GCAGTG	1 cut(s)	1501
BtsIMutI	CAGTG	6 cut(s)	1501, 1653, 2171, 2244, 2984, 3022
BveI	ACCTGC	2 cut(s)	475, 934
Cac8I	GCNNGC	7 cut(s)	2196, 2200, 2274, 2409, 2632, 3320, 3324
CaiI	CAGNNNCTG	2 cut(s)	1652, 1748
CciI	TCATGA	1 cut(s)	2692
CfoI	GCGC	3 cut(s)	1145, 2992, 3510
Cfr13I	GGNCC	7 cut(s)	464, 1136, 1444, 1520, 1981, 2003, 3454
ClaI	ATCGAT	1 cut(s)	1349
CseI	GACGC	1 cut(s)	1304
Csp6I	GTAC	6 cut(s)	642, 723, 1196, 2836, 3234, 3245
CspCI	CAANNNNNGTGG	2 cut(s)	1869, 1904
CviQI	GTAC	6 cut(s)	642, 723, 1196, 2836, 3234, 3245
EaeI	YGGCCR	1 cut(s)	2632
Eam1104I	CTCTTC	9 cut(s)	162, 591, 955, 1047, 1225, 1713, 2475, 2526, 3254
EarI	CTCTTC	9 cut(s)	162, 591, 955, 1047, 1225, 1713, 2475, 2526, 3254
EciI	GGCGGA	1 cut(s)	50
Eco130I	CCWWGG	6 cut(s)	726, 758, 840, 1061, 3010, 3457
Eco147I	AGGCCT	1 cut(s)	1249
Eco31I	GGTCTC	1 cut(s)	2534
Eco32I	GATATC	2 cut(s)	528, 1915
Eco47I	GGWCC	5 cut(s)	464, 1136, 1520, 1981, 3454
Eco57I	CTGAAG	8 cut(s)	134, 576, 777, 1206, 2268, 2978, 3543, 3551
Eco81I	CCTNAGG	2 cut(s)	696, 3159
EcoNI	CCTNNNNNAGG	2 cut(s)	567, 1254
EcoRI	GAATTC	2 cut(s)	662, 2617
EcoRII	CCWGG	3 cut(s)	874, 1138, 2806
EcoRV	GATATC	2 cut(s)	528, 1915
EcoT14I	CCWWGG	6 cut(s)	726, 758, 840, 1061, 3010, 3457
ErhI	CCWWGG	6 cut(s)	726, 758, 840, 1061, 3010, 3457
Esp3I	CGTCTC	2 cut(s)	1125, 3104
FalI	AAGNNNNNCTT	4 cut(s)	1710, 1742, 3413, 3445
FaqI	GGGAC	6 cut(s)	221, 391, 1382, 1994, 3186, 3467
FauI	CCCGC	2 cut(s)	1923, 2172
FauNDI	CATATG	1 cut(s)	3181
FbaI	TGATCA	3 cut(s)	1543, 2755, 3310
FblI	GTMKAC	1 cut(s)	2940
FokI	GGATG	8 cut(s)	1205, 1550, 1727, 1865, 2339, 2489, 2726, 3412
GlaI	GCGC	3 cut(s)	1144, 2991, 3509
GsaI	CCCAGC	1 cut(s)	378
HaeII	RGCGCY	2 cut(s)	2993, 3511
HaeIII	GGCC	6 cut(s)	1249, 1445, 1828, 2005, 2634, 3009
HgaI	GACGC	1 cut(s)	1304
HhaI	GCGC	3 cut(s)	1145, 2992, 3510
Hin6I	GCGC	3 cut(s)	1143, 2990, 3508
HinP1I	GCGC	3 cut(s)	1143, 2990, 3508
HincII	GTYRAC	2 cut(s)	52, 855
HindII	GTYRAC	2 cut(s)	52, 855
HindIII	AAGCTT	2 cut(s)	155, 2777
HphI	GGTGA	8 cut(s)	266, 626, 857, 1343, 1592, 2298, 2822, 3298
Hpy166II	GTNNAC	7 cut(s)	52, 855, 2078, 2210, 2563, 2879, 2941
Hpy8I	GTNNAC	7 cut(s)	52, 855, 2078, 2210, 2563, 2879, 2941
HpyCH4III	ACNGT	6 cut(s)	424, 934, 1327, 2214, 3026, 3065
HpyCH4IV	ACGT	6 cut(s)	54, 1831, 2038, 2790, 3132, 3334
HpyF10VI	GCNNNNNNNGC	9 cut(s)	101, 115, 1166, 1295, 2413, 2687, 2959, 3524, 3541
HpySE526I	ACGT	6 cut(s)	54, 1831, 2038, 2790, 3132, 3334
HspAI	GCGC	3 cut(s)	1143, 2990, 3508
Ksp22I	TGATCA	3 cut(s)	1543, 2755, 3310
LguI	GCTCTTC	1 cut(s)	955
LmnI	GCTCC	6 cut(s)	349, 877, 940, 1723, 3131, 3160
LweI	GCATC	5 cut(s)	1307, 1894, 2474, 3225, 3527
MaeII	ACGT	6 cut(s)	54, 1831, 2038, 2790, 3132, 3334
MbiI	CCGCTC	2 cut(s)	352, 1585
MfeI	CAATTG	2 cut(s)	1706, 2186
MflI	RGATCY	5 cut(s)	239, 1504, 1963, 2351, 3143
MhlI	GDGCHC	4 cut(s)	874, 2409, 2565, 3157
MlyI	GAGTC	5 cut(s)	32, 1364, 2018, 2309, 2933
MmeI	TCCRAC	3 cut(s)	171, 1381, 1547
MroXI	GAANNNNTTC	4 cut(s)	143, 1974, 2317, 3298
MseI	TTAA	8 cut(s)	714, 974, 1107, 1685, 1728, 1733, 2867, 2916
MslI	CAYNNNNRTG	2 cut(s)	1494, 2714
MspCI	CTTAAG	2 cut(s)	713, 973
MspR9I	CCNGG	3 cut(s)	876, 1140, 2808
MunI	CAATTG	2 cut(s)	1706, 2186
Mva1269I	GAATGC	2 cut(s)	398, 2669
MvaI	CCWGG	3 cut(s)	876, 1140, 2808
MvnI	CGCG	1 cut(s)	827
MwoI	GCNNNNNNNGC	9 cut(s)	101, 115, 1166, 1295, 2413, 2687, 2959, 3524, 3541
NcoI	CCATGG	1 cut(s)	726
NdeI	CATATG	1 cut(s)	3181
NheI	GCTAGC	1 cut(s)	3322
NlaIV	GGNNCC	3 cut(s)	1521, 1982, 3455
NmuCI	GTSAC	8 cut(s)	671, 981, 1466, 1648, 1765, 2242, 2810, 3026
NspI	RCATGY	3 cut(s)	2272, 3322, 3370
PaeI	GCATGC	1 cut(s)	3322
PagI	TCATGA	1 cut(s)	2692
PceI	AGGCCT	1 cut(s)	1249
PciSI	GCTCTTC	1 cut(s)	955
PctI	GAATGC	2 cut(s)	398, 2669
PdmI	GAANNNNTTC	4 cut(s)	143, 1974, 2317, 3298
PleI	GAGTC	5 cut(s)	32, 1364, 2018, 2308, 2932
PpsI	GAGTC	5 cut(s)	32, 1364, 2018, 2308, 2932
PshAI	GACNNNNGTC	1 cut(s)	2962
PsiI	TTATAA	2 cut(s)	1619, 2732
Psp6I	CCWGG	3 cut(s)	874, 1138, 2806
PspFI	CCCAGC	1 cut(s)	374
PspGI	CCWGG	3 cut(s)	874, 1138, 2806
PspN4I	GGNNCC	3 cut(s)	1521, 1982, 3455
PspPI	GGNCC	7 cut(s)	464, 1136, 1444, 1520, 1981, 2003, 3454
PstI	CTGCAG	2 cut(s)	472, 2964
PstNI	CAGNNNCTG	2 cut(s)	1652, 1748
PsuI	RGATCY	5 cut(s)	239, 1504, 1963, 2351, 3143
RsaI	GTAC	6 cut(s)	643, 724, 1197, 2837, 3235, 3246
RsaNI	GTAC	6 cut(s)	642, 723, 1196, 2836, 3234, 3245
RseI	CAYNNNNRTG	2 cut(s)	1494, 2714
SapI	GCTCTTC	1 cut(s)	955
SaqAI	TTAA	8 cut(s)	714, 974, 1107, 1685, 1728, 1733, 2867, 2916
Sau96I	GGNCC	7 cut(s)	464, 1136, 1444, 1520, 1981, 2003, 3454
ScaI	AGTACT	1 cut(s)	643
SchI	GAGTC	5 cut(s)	32, 1364, 2018, 2309, 2933
ScrFI	CCNGG	3 cut(s)	876, 1140, 2808
SduI	GDGCHC	4 cut(s)	874, 2409, 2565, 3157
SfaNI	GCATC	5 cut(s)	1307, 1894, 2474, 3225, 3527
SfcI	CTRYAG	5 cut(s)	468, 1251, 1635, 2960, 3061
SinI	GGWCC	5 cut(s)	464, 1136, 1520, 1981, 3454
SmiMI	CAYNNNNRTG	2 cut(s)	1494, 2714
SmlI	CTYRAG	9 cut(s)	713, 973, 1082, 2051, 2471, 2523, 2552, 3430, 3488
SmoI	CTYRAG	9 cut(s)	713, 973, 1082, 2051, 2471, 2523, 2552, 3430, 3488
SpeI	ACTAGT	1 cut(s)	2072
SphI	GCATGC	1 cut(s)	3322
SseBI	AGGCCT	1 cut(s)	1249
StuI	AGGCCT	1 cut(s)	1249
StyD4I	CCNGG	3 cut(s)	874, 1138, 2806
StyI	CCWWGG	6 cut(s)	726, 758, 840, 1061, 3010, 3457
TaaI	ACNGT	6 cut(s)	424, 934, 1327, 2214, 3026, 3065
TaiI	ACGT	6 cut(s)	57, 1834, 2041, 2793, 3135, 3337
TaqI	TCGA	4 cut(s)	660, 771, 1349, 2854
TatI	WGTACW	3 cut(s)	641, 3233, 3244
TauI	GCSGC	3 cut(s)	2170, 2489, 2637
Tru1I	TTAA	8 cut(s)	714, 974, 1107, 1685, 1728, 1733, 2867, 2916
Tru9I	TTAA	8 cut(s)	714, 974, 1107, 1685, 1728, 1733, 2867, 2916
TscAI	CASTG	6 cut(s)	1501, 1653, 2178, 2251, 2984, 3029
TseFI	GTSAC	8 cut(s)	671, 981, 1466, 1648, 1765, 2242, 2810, 3026
Tsp45I	GTSAC	8 cut(s)	671, 981, 1466, 1648, 1765, 2242, 2810, 3026
TspGWI	ACGGA	4 cut(s)	805, 1149, 3128, 3394
TspRI	CASTG	6 cut(s)	1501, 1653, 2178, 2251, 2984, 3029
Vha464I	CTTAAG	2 cut(s)	713, 973
VneI	GTGCAC	1 cut(s)	2561
VpaK11BI	GGWCC	5 cut(s)	464, 1136, 1520, 1981, 3454
XagI	CCTNNNNNAGG	2 cut(s)	567, 1254
XapI	RAATTY	5 cut(s)	662, 2599, 2617, 2903, 2912
XbaI	TCTAGA	2 cut(s)	1966, 2983
XceI	RCATGY	3 cut(s)	2272, 3322, 3370
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1107
XmaJI	CCTAGG	1 cut(s)	1061
XmiI	GTMKAC	1 cut(s)	2940
XmnI	GAANNNNTTC	4 cut(s)	143, 1974, 2317, 3298
ZrmI	AGTACT	1 cut(s)	643

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.