MD06G1241600.v1.1

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0003480
Physical Location & Seq
Reverse (-)
37123608 .. 37129969
6362 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1241600.v1.1.491

Sequence Viewer

Length: 1125 bp
ATGGTAGTGAGCATTGCCCCTTATTGCTTGAGTTTTGCTCAACAAGCCTGTCATTCAAACAACATTTCAAAACATATAACTTTAGGTAACTATGCAAGAGACACAAGGAGAGGTTGTATTAGAAGGCCTCAGATTTTGCCTTCTGCTCTTGGACATGCTGTTGGGTTACGTGTGTTTGTGCTCTCTGACTTGCACACTGACTATTCTGAGAACATGGAATGGGTCAAGTGCTTACCAACTGTCAGATACAAGAATGATACTCTTATTGTTGCGGGTGATGTCGCTGAGACATACAACAACTTTATCGTGACAATGTCTGCCTTGAAGGATAGATTTGAACATGTTTTGTATGTGCCTGGAAACCATGACCTTTGGTGCCGTCAGGAGGGAGAAATTCATCTTGATTCTCATCAGAAATTAAAGAAATTGCTTGATGCGTGTAGAGGACTTGGAGTTGAGACCGGTCCGATGGTTTTAGATGGCTTAGGAATCATTCCTTTGTTCTCATGGTACCATGAGAGCTTTGATAGGGAGGAGGAAATAACCAGCACCCACATTCTGTCTTTGGAGATGGCATGTAAAGACTTCAAAGCATGCAAGTGGTCCAAGGAACTGTCAAATGGAGATATCTCACTTGCCCTCTACTTTGATGCAATGAATGAAAAGAATCAGAGTCTGATCAAGGAGATCCAAAACACATGTAGCCAAATAATTTCATTCTCTCATTTTGTTCCCAGGCAAGAGCTATTTCCTGAGAAAAGGATGCTATTCTATCCAAAGCTTCCAAAGATTATTGGTTCTGATTGTCTTGAGGCCCGTATACGGTCAATCCATGGGGTTGAAGGAAGTCCATCTGCATGTCATGTGTTTGGTCATACACATTTCTGTTGGGATTCTGTCATTCATGGTATCAGGTATGTGCAGGCGCCACTGGCTTACCCAAGAGAACGGAAGAGGAGAATGAATGGGGGTGAAAATTGGTTGCCATTTTGTATCTATAGTAACGGAAAGTTTTCAGAGAAGGTTTCACCGTGTTATTGGTCTGATTATTACTCTACCCACCCAAGAACACCTCATAACACTCAACTCGCTCCTTGGGTTGCCACATTTTACAACCAAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

375

Amino Acids

42.9

Weight (kDa)

7.59

Isoelectric Point (pI)

47.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 56 - 135 1e-07 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 510
AccB1I GGYRCC 3 cut(s) 375, 510, 925
AccI GTMKAC 1 cut(s) 820
AciI CCGC 1 cut(s) 272
AclWI GGATC 1 cut(s) 682
AcsI RAATTY 1 cut(s) 393
AcyI GRCGYC 1 cut(s) 926
AfaI GTAC 1 cut(s) 512
AfiI CCNNNNNNNGG 2 cut(s) 385, 822
AflIII ACRYGT 3 cut(s) 169, 340, 698
AgeI ACCGGT 1 cut(s) 461
AgsI TTSAA 6 cut(s) 57, 69, 325, 338, 589, 842
AjnI CCWGG 2 cut(s) 355, 734
AluBI AGCT 3 cut(s) 522, 745, 781
AluI AGCT 3 cut(s) 522, 745, 781
Alw21I GWGCWC 1 cut(s) 183
Alw26I GTCTC 3 cut(s) 93, 281, 452
AlwI GGATC 1 cut(s) 682
AlwNI CAGNNNCTG 1 cut(s) 676
AoxI GGCC 2 cut(s) 125, 813
ApoI RAATTY 1 cut(s) 393
AsiGI ACCGGT 1 cut(s) 461
Asp700I GAANNNNTTC 1 cut(s) 1012
Asp718I GGTACC 1 cut(s) 510
AspLEI GCGC 1 cut(s) 928
AspS9I GGNCC 3 cut(s) 464, 603, 814
AsuHPI GGTGA 3 cut(s) 287, 983, 1020
AvaII GGWCC 2 cut(s) 464, 603
BanI GGYRCC 3 cut(s) 375, 510, 925
Bbv12I GWGCWC 1 cut(s) 183
BccI CCATC 4 cut(s) 463, 473, 565, 859
BceAI ACGGC 1 cut(s) 363
BciT130I CCWGG 2 cut(s) 357, 736
BclI TGATCA 1 cut(s) 678
BcoDI GTCTC 3 cut(s) 93, 281, 452
BfmI CTRYAG 1 cut(s) 997
BfoI RGCGCY 1 cut(s) 929
Bme1390I CCNGG 2 cut(s) 357, 736
Bme18I GGWCC 2 cut(s) 464, 603
BmgT120I GGNCC 3 cut(s) 464, 603, 814
BmiI GGNNCC 3 cut(s) 377, 512, 927
BmrFI CCNGG 2 cut(s) 357, 736
BmsI GCATC 3 cut(s) 424, 640, 753
BplI GAGNNNNNCTC 2 cut(s) 22, 54
Bpu10I CCTNAGC 1 cut(s) 484
BpuEI CTTGAG 2 cut(s) 49, 830
BsaAI YACGTR 1 cut(s) 170
BsaBI GATNNNNATC 1 cut(s) 408
BsaHI GRCGYC 1 cut(s) 926
BsaI GGTCTC 1 cut(s) 452
BsaJI CCNNGG 4 cut(s) 606, 734, 832, 1094
BsaWI WCCGGW 1 cut(s) 461
BsaXI ACNNNNNCTCC 2 cut(s) 100, 130
Bsc4I CCNNNNNNNGG 2 cut(s) 385, 822
Bse118I RCCGGY 1 cut(s) 461
Bse1I ACTGG 1 cut(s) 936
Bse3DI GCAATG 2 cut(s) 12, 660
Bse8I GATNNNNATC 1 cut(s) 408
BseBI CCWGG 2 cut(s) 357, 736
BseDI CCNNGG 4 cut(s) 606, 734, 832, 1094
BseGI GGATG 1 cut(s) 768
BseJI GATNNNNATC 1 cut(s) 408
BseLI CCNNNNNNNGG 2 cut(s) 385, 822
BseMI GCAATG 2 cut(s) 12, 660
BseMII CTCAG 4 cut(s) 143, 198, 276, 744
BseNI ACTGG 1 cut(s) 936
BseRI GAGGAG 2 cut(s) 548, 970
BsgI GTGCAG 1 cut(s) 941
BshFI GGCC 2 cut(s) 127, 815
BshNI GGYRCC 3 cut(s) 375, 510, 925
BshTI ACCGGT 1 cut(s) 461
BsiHKAI GWGCWC 1 cut(s) 183
BsiSI CCGG 1 cut(s) 462
BslI CCNNNNNNNGG 2 cut(s) 385, 822
BsmAI GTCTC 3 cut(s) 93, 281, 452
BsnI GGCC 2 cut(s) 127, 815
Bso31I GGTCTC 1 cut(s) 452
Bsp1286I GDGCHC 1 cut(s) 183
Bsp143I GATC 2 cut(s) 678, 687
Bsp19I CCATGG 1 cut(s) 832
BspACI CCGC 1 cut(s) 272
BspANI GGCC 2 cut(s) 127, 815
BspCNI CTCAG 4 cut(s) 142, 199, 277, 745
BspLI GGNNCC 3 cut(s) 377, 512, 927
BspPI GGATC 1 cut(s) 682
BspT107I GGYRCC 3 cut(s) 375, 510, 925
BspTNI GGTCTC 1 cut(s) 452
BsrDI GCAATG 2 cut(s) 12, 660
BsrFI RCCGGY 1 cut(s) 461
BsrI ACTGG 1 cut(s) 936
BssAI RCCGGY 1 cut(s) 461
BssECI CCNNGG 4 cut(s) 606, 734, 832, 1094
BssMI GATC 2 cut(s) 678, 687
BssNAI GTATAC 1 cut(s) 821
BssNI GRCGYC 1 cut(s) 926
BssT1I CCWWGG 3 cut(s) 606, 832, 1094
Bst1107I GTATAC 1 cut(s) 821
Bst2UI CCWGG 2 cut(s) 357, 736
Bst4CI ACNGT 4 cut(s) 241, 615, 825, 1032
Bst6I CTCTTC 1 cut(s) 947
BstACI GRCGYC 1 cut(s) 926
BstBAI YACGTR 1 cut(s) 170
BstC8I GCNNGC 2 cut(s) 595, 924
BstDEI CTNAG 5 cut(s) 129, 207, 285, 484, 753
BstDSI CCRYGG 1 cut(s) 832
BstF5I GGATG 1 cut(s) 768
BstH2I RGCGCY 1 cut(s) 929
BstHHI GCGC 1 cut(s) 928
BstKTI GATC 2 cut(s) 681, 690
BstMAI GTCTC 3 cut(s) 93, 281, 452
BstMBI GATC 2 cut(s) 678, 687
BstMWI GCNNNNNNNGC 2 cut(s) 44, 932
BstNI CCWGG 2 cut(s) 357, 736
BstNSI RCATGY 6 cut(s) 158, 344, 579, 597, 702, 861
BstSCI CCNGG 2 cut(s) 355, 734
BstSFI CTRYAG 1 cut(s) 997
BstX2I RGATCY 1 cut(s) 687
BstYI RGATCY 1 cut(s) 687
BstZ17I GTATAC 1 cut(s) 821
BsuRI GGCC 2 cut(s) 127, 815
BtgI CCRYGG 1 cut(s) 832
BtsCI GGATG 1 cut(s) 768
BtsIMutI CAGTG 2 cut(s) 195, 929
Cac8I GCNNGC 2 cut(s) 595, 924
CaiI CAGNNNCTG 1 cut(s) 676
CfoI GCGC 1 cut(s) 928
Cfr10I RCCGGY 1 cut(s) 461
Cfr13I GGNCC 3 cut(s) 464, 603, 814
CpoI CGGWCCG 1 cut(s) 464
Csp6I GTAC 1 cut(s) 511
CspAI ACCGGT 1 cut(s) 461
CspI CGGWCCG 1 cut(s) 464
CviJI RGCY 9 cut(s) 47, 127, 483, 522, 705, 745, 781, 815, 935
CviKI_1 RGCY 9 cut(s) 47, 127, 483, 522, 705, 745, 781, 815, 935
CviQI GTAC 1 cut(s) 511
DdeI CTNAG 5 cut(s) 129, 207, 285, 484, 753
DinI GGCGCC 1 cut(s) 927
DpnI GATC 2 cut(s) 680, 689
DpnII GATC 2 cut(s) 678, 687
Eam1104I CTCTTC 1 cut(s) 947
EarI CTCTTC 1 cut(s) 947
Eco130I CCWWGG 3 cut(s) 606, 832, 1094
Eco147I AGGCCT 1 cut(s) 127
Eco31I GGTCTC 1 cut(s) 452
Eco32I GATATC 1 cut(s) 628
Eco47I GGWCC 2 cut(s) 464, 603
EcoRII CCWGG 2 cut(s) 355, 734
EcoRV GATATC 1 cut(s) 628
EcoT14I CCWWGG 3 cut(s) 606, 832, 1094
EgeI GGCGCC 1 cut(s) 927
EheI GGCGCC 1 cut(s) 927
ErhI CCWWGG 3 cut(s) 606, 832, 1094
FauI CCCGC 1 cut(s) 265
FbaI TGATCA 1 cut(s) 678
FblI GTMKAC 1 cut(s) 820
FokI GGATG 1 cut(s) 775
GlaI GCGC 1 cut(s) 927
HaeII RGCGCY 1 cut(s) 929
HaeIII GGCC 2 cut(s) 127, 815
HapII CCGG 1 cut(s) 462
HhaI GCGC 1 cut(s) 928
Hin1I GRCGYC 1 cut(s) 926
Hin6I GCGC 1 cut(s) 926
HinP1I GCGC 1 cut(s) 926
HindIII AAGCTT 1 cut(s) 779
HinfI GANTC 5 cut(s) 404, 489, 667, 673, 893
HpaII CCGG 1 cut(s) 462
HphI GGTGA 3 cut(s) 287, 983, 1020
Hpy166II GTNNAC 1 cut(s) 821
Hpy188III TCNNGA 5 cut(s) 307, 383, 401, 752, 809
Hpy8I GTNNAC 1 cut(s) 821
HpyAV CCTTC 5 cut(s) 117, 150, 319, 836, 1015
HpyCH4III ACNGT 4 cut(s) 241, 615, 825, 1032
HpyCH4IV ACGT 1 cut(s) 169
HpyCH4V TGCA 6 cut(s) 95, 193, 597, 653, 857, 922
HpyF10VI GCNNNNNNNGC 2 cut(s) 44, 932
HpyF3I CTNAG 5 cut(s) 129, 207, 285, 484, 753
HpySE526I ACGT 1 cut(s) 169
Hsp92I GRCGYC 1 cut(s) 926
HspAI GCGC 1 cut(s) 926
KasI GGCGCC 1 cut(s) 925
KpnI GGTACC 1 cut(s) 514
Ksp22I TGATCA 1 cut(s) 678
Kzo9I GATC 2 cut(s) 678, 687
LmnI GCTCC 1 cut(s) 1096
LweI GCATC 3 cut(s) 424, 640, 753
MaeII ACGT 1 cut(s) 169
MaeIII GTNAC 4 cut(s) 86, 165, 307, 1001
MalI GATC 2 cut(s) 680, 689
MboI GATC 2 cut(s) 678, 687
MboII GAAGA 1 cut(s) 964
MflI RGATCY 1 cut(s) 687
MhlI GDGCHC 1 cut(s) 183
MluCI AATT 5 cut(s) 393, 416, 425, 711, 976
Mly113I GGCGCC 1 cut(s) 926
MlyI GAGTC 1 cut(s) 682
MroXI GAANNNNTTC 1 cut(s) 1012
MseI TTAA 1 cut(s) 419
MslI CAYNNNNRTG 2 cut(s) 598, 856
MspI CCGG 1 cut(s) 462
MspR9I CCNGG 2 cut(s) 357, 736
MvaI CCWGG 2 cut(s) 357, 736
MwoI GCNNNNNNNGC 2 cut(s) 44, 932
NarI GGCGCC 1 cut(s) 926
NcoI CCATGG 1 cut(s) 832
NdeII GATC 2 cut(s) 678, 687
NlaIV GGNNCC 3 cut(s) 377, 512, 927
NmuCI GTSAC 1 cut(s) 307
NspI RCATGY 6 cut(s) 158, 344, 579, 597, 702, 861
PaeI GCATGC 1 cut(s) 597
PceI AGGCCT 1 cut(s) 127
PciI ACATGT 2 cut(s) 340, 698
PdmI GAANNNNTTC 1 cut(s) 1012
PfeI GAWTC 4 cut(s) 404, 489, 667, 893
PflFI GACNNNGTC 1 cut(s) 313
PinAI ACCGGT 1 cut(s) 461
PleI GAGTC 1 cut(s) 681
PluTI GGCGCC 1 cut(s) 929
PpsI GAGTC 1 cut(s) 681
Ppu21I YACGTR 1 cut(s) 170
PscI ACATGT 2 cut(s) 340, 698
Psp6I CCWGG 2 cut(s) 355, 734
PspGI CCWGG 2 cut(s) 355, 734
PspN4I GGNNCC 3 cut(s) 377, 512, 927
PspPI GGNCC 3 cut(s) 464, 603, 814
PstNI CAGNNNCTG 1 cut(s) 676
PsuI RGATCY 1 cut(s) 687
PsyI GACNNNGTC 1 cut(s) 313
RsaI GTAC 1 cut(s) 512
RsaNI GTAC 1 cut(s) 511
RseI CAYNNNNRTG 2 cut(s) 598, 856
Rsr2I CGGWCCG 1 cut(s) 464
RsrII CGGWCCG 1 cut(s) 464
SaqAI TTAA 1 cut(s) 419
Sau3AI GATC 2 cut(s) 678, 687
Sau96I GGNCC 3 cut(s) 464, 603, 814
SchI GAGTC 1 cut(s) 682
ScrFI CCNGG 2 cut(s) 357, 736
SduI GDGCHC 1 cut(s) 183
SfaNI GCATC 3 cut(s) 424, 640, 753
SfcI CTRYAG 1 cut(s) 997
SfoI GGCGCC 1 cut(s) 927
SinI GGWCC 2 cut(s) 464, 603
SmiMI CAYNNNNRTG 2 cut(s) 598, 856
SmlI CTYRAG 2 cut(s) 28, 809
SmoI CTYRAG 2 cut(s) 28, 809
SphI GCATGC 1 cut(s) 597
Sse9I AATT 5 cut(s) 393, 416, 425, 711, 976
SseBI AGGCCT 1 cut(s) 127
SsiI CCGC 1 cut(s) 272
SspDI GGCGCC 1 cut(s) 925
StuI AGGCCT 1 cut(s) 127
StyD4I CCNGG 2 cut(s) 355, 734
StyI CCWWGG 3 cut(s) 606, 832, 1094
TaaI ACNGT 4 cut(s) 241, 615, 825, 1032
TaiI ACGT 1 cut(s) 172
TasI AATT 5 cut(s) 393, 416, 425, 711, 976
TfiI GAWTC 4 cut(s) 404, 489, 667, 893
Tru1I TTAA 1 cut(s) 419
Tru9I TTAA 1 cut(s) 419
TscAI CASTG 2 cut(s) 202, 936
TseFI GTSAC 1 cut(s) 307
Tsp45I GTSAC 1 cut(s) 307
TspDTI ATGAA 6 cut(s) 386, 671, 675, 705, 893, 977
TspGWI ACGGA 2 cut(s) 964, 1020
TspRI CASTG 2 cut(s) 202, 936
Tth111I GACNNNGTC 1 cut(s) 313
VpaK11BI GGWCC 2 cut(s) 464, 603
XapI RAATTY 1 cut(s) 393
XceI RCATGY 6 cut(s) 158, 344, 579, 597, 702, 861
XmiI GTMKAC 1 cut(s) 820
XmnI GAANNNNTTC 1 cut(s) 1012
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.