Rw7G000050

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0003480
Physical Location & Seq
Forward (+)
107343 .. 112034
4692 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G000050.1

Sequence Viewer

Length: 1191 bp
ATGGTACCAAGTGTTGCCATATCTTGCTTGAGCTTATCGCAATATGCTTATCATTCAAACCACATGTCAAAACATATATCATTGAGTAAATATAGAAGAGACATAAGGAGGGGTTGCATTACAAGGTCTCAAATTTTGCCTTCAGTTCTTGGGGATGCTGATGGGTTACGAGTATTTGTGCTCTCAGATTTGCACACTGACTATACTGAAAACATGAAATGGATCAAGGACTTATCGTCCGTCAAATACAAGAATGATGTTCTTATTGTTGCTGGTGATGTTTCTGAGACATACAAGAACTTTATAATGACCATGTCTCTCTTGAAGAATAAATTTGAACATGTCCTTTATGTTCCTGGGAACCATGACCTCTGGTGCCGTCGGGAGGGAGAAATTTATCTTGATTCTCATCAGAAGTTAAATAAAGTGCTAGATGCGTGTAGAGGACTTGGAGTTGAGACAAATCCAATAGTTTTAGATGGCTTGGGAATTATTCCTTTGCTCTCATGGTATCATGAGAGCTTTGATAAGGAGGAGGAAATAACCAATATCCACATTCCACCTCTAGAGATGGCATGCAAGGACTTCAAGGCATGCAAATGGTCTGGGGAACTGTCAAATGGAGACACCTCGCTTGCTCTGTATTTCGATGCAATGAATGAGAAGAATCAAAACGTGATCAAGGAGATCCAGAACACATGTAGCCAAATAATTACCTTTTCTCACTTTGTCCCCAGGCAAGAGCTGTGTCCAGAGAAAAGAATGCTATTCTATCCAAAGCTTCCAAAGATTATTGGCTCTGATTGTCTTGAGGTCCGTATAAGGTCCATCCATGGGGTTGAAGGAAGTGCATCTGCATGTCATGTGTTTGGTCATACACATTTCTGCTGGGATGCTGTGCTTGATGGTATCAGGTATGTGCAGGCTCCATTAGCTTACCCAAGGGAACGGAAGAGGAGAATGAATGGGGGTGAAAATTGGTTGCCATTTTGTATCTATTCTAATGGAAAGTTGTCTGATAGGCTTTCGCCGTGTTATTGGTCTGATTATTACTCTTCCAACCCAAGAACACCTCATAACACTAAACTTGCCCCTTGGGTTGCCAGAATTGGAGATCCATACGATCATGCTAAACATTCAAAGCCCTATAAGAGGTTGATGGAACTACATGGAGATCTAGCAGCAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

396

Amino Acids

45.45

Weight (kDa)

8.31

Isoelectric Point (pI)

45.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 56 - 137 1.4e-09 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 305
Acc65I GGTACC 1 cut(s) 4
AccB1I GGYRCC 2 cut(s) 4, 375
AclWI GGATC 3 cut(s) 230, 682, 1109
AcsI RAATTY 3 cut(s) 132, 332, 393
AcuI CTGAAG 1 cut(s) 126
AfaI GTAC 1 cut(s) 6
AfiI CCNNNNNNNGG 3 cut(s) 385, 834, 1152
AflIII ACRYGT 3 cut(s) 63, 340, 698
AgsI TTSAA 6 cut(s) 57, 325, 338, 589, 842, 1140
AhdI GACNNNNNGTC 1 cut(s) 235
AjnI CCWGG 2 cut(s) 355, 734
AluBI AGCT 5 cut(s) 33, 522, 745, 781, 935
AluI AGCT 5 cut(s) 33, 522, 745, 781, 935
Alw21I GWGCWC 1 cut(s) 183
Alw26I GTCTC 6 cut(s) 93, 132, 281, 321, 452, 618
AlwI GGATC 3 cut(s) 230, 682, 1109
ApeKI GCWGC 1 cut(s) 1181
ApoI RAATTY 3 cut(s) 132, 332, 393
ArsI GACNNNNNNTTYG 2 cut(s) 50, 82
Asp718I GGTACC 1 cut(s) 4
AspS9I GGNCC 2 cut(s) 814, 825
AsuHPI GGTGA 2 cut(s) 287, 983
AvaII GGWCC 2 cut(s) 814, 825
BanI GGYRCC 2 cut(s) 4, 375
Bbv12I GWGCWC 1 cut(s) 183
BccI CCATC 6 cut(s) 155, 473, 565, 836, 899, 1153
BceAI ACGGC 2 cut(s) 363, 1015
BciT130I CCWGG 2 cut(s) 357, 736
BclI TGATCA 1 cut(s) 678
BcoDI GTCTC 6 cut(s) 93, 132, 281, 321, 452, 618
BfaI CTAG 3 cut(s) 431, 566, 1178
BglII AGATCT 1 cut(s) 1174
BisI GCNGC 1 cut(s) 1182
BlsI GCNGC 1 cut(s) 1183
Bme1390I CCNGG 2 cut(s) 357, 736
Bme18I GGWCC 2 cut(s) 814, 825
BmeRI GACNNNNNGTC 1 cut(s) 235
BmgT120I GGNCC 2 cut(s) 814, 825
BmiI GGNNCC 4 cut(s) 6, 362, 377, 927
BmrFI CCNGG 2 cut(s) 357, 736
BmsI GCATC 5 cut(s) 145, 424, 640, 860, 883
BpuEI CTTGAG 2 cut(s) 49, 830
BsaBI GATNNNNATC 1 cut(s) 408
BsaI GGTCTC 1 cut(s) 132
BsaJI CCNNGG 5 cut(s) 356, 734, 832, 941, 1094
Bsc4I CCNNNNNNNGG 3 cut(s) 385, 834, 1152
Bse3DI GCAATG 1 cut(s) 660
Bse8I GATNNNNATC 1 cut(s) 408
BseBI CCWGG 2 cut(s) 357, 736
BseDI CCNNGG 5 cut(s) 356, 734, 832, 941, 1094
BseGI GGATG 3 cut(s) 160, 828, 898
BseJI GATNNNNATC 1 cut(s) 408
BseLI CCNNNNNNNGG 3 cut(s) 385, 834, 1152
BseMI GCAATG 1 cut(s) 660
BseMII CTCAG 2 cut(s) 198, 276
BseRI GAGGAG 2 cut(s) 548, 970
BseYI CCCAGC 1 cut(s) 888
BsgI GTGCAG 1 cut(s) 941
BshNI GGYRCC 2 cut(s) 4, 375
BsiHKAI GWGCWC 1 cut(s) 183
BslFI GGGAC 1 cut(s) 716
BslI CCNNNNNNNGG 3 cut(s) 385, 834, 1152
BsmAI GTCTC 6 cut(s) 93, 132, 281, 321, 452, 618
BsmFI GGGAC 1 cut(s) 716
BsmI GAATGC 1 cut(s) 768
Bso31I GGTCTC 1 cut(s) 132
Bsp1286I GDGCHC 1 cut(s) 183
Bsp143I GATC 6 cut(s) 222, 678, 687, 1114, 1123, 1174
Bsp19I CCATGG 1 cut(s) 832
BspCNI CTCAG 2 cut(s) 197, 277
BspHI TCATGA 1 cut(s) 514
BspLI GGNNCC 4 cut(s) 6, 362, 377, 927
BspPI GGATC 3 cut(s) 230, 682, 1109
BspT107I GGYRCC 2 cut(s) 4, 375
BspTNI GGTCTC 1 cut(s) 132
BsrDI GCAATG 1 cut(s) 660
BssECI CCNNGG 5 cut(s) 356, 734, 832, 941, 1094
BssMI GATC 6 cut(s) 222, 678, 687, 1114, 1123, 1174
BssT1I CCWWGG 3 cut(s) 832, 941, 1094
Bst2UI CCWGG 2 cut(s) 357, 736
Bst4CI ACNGT 1 cut(s) 615
Bst6I CTCTTC 3 cut(s) 91, 947, 1060
BstC8I GCNNGC 4 cut(s) 577, 595, 636, 924
BstDEI CTNAG 2 cut(s) 184, 285
BstDSI CCRYGG 1 cut(s) 832
BstENI CCTNNNNNAGG 1 cut(s) 1150
BstF5I GGATG 3 cut(s) 160, 828, 898
BstKTI GATC 6 cut(s) 225, 681, 690, 1117, 1126, 1177
BstMAI GTCTC 6 cut(s) 93, 132, 281, 321, 452, 618
BstMBI GATC 6 cut(s) 222, 678, 687, 1114, 1123, 1174
BstMWI GCNNNNNNNGC 1 cut(s) 932
BstNI CCWGG 2 cut(s) 357, 736
BstNSI RCATGY 6 cut(s) 67, 344, 579, 597, 702, 861
BstSCI CCNGG 2 cut(s) 355, 734
BstX2I RGATCY 3 cut(s) 687, 1114, 1174
BstYI RGATCY 3 cut(s) 687, 1114, 1174
BtgI CCRYGG 1 cut(s) 832
BtsCI GGATG 3 cut(s) 160, 828, 898
BtsIMutI CAGTG 1 cut(s) 195
Cac8I GCNNGC 4 cut(s) 577, 595, 636, 924
CciI TCATGA 1 cut(s) 514
Cfr13I GGNCC 2 cut(s) 814, 825
Csp6I GTAC 1 cut(s) 5
CviQI GTAC 1 cut(s) 5
DdeI CTNAG 2 cut(s) 184, 285
DpnI GATC 6 cut(s) 224, 680, 689, 1116, 1125, 1176
DpnII GATC 6 cut(s) 222, 678, 687, 1114, 1123, 1174
DriI GACNNNNNGTC 1 cut(s) 235
Eam1104I CTCTTC 3 cut(s) 91, 947, 1060
Eam1105I GACNNNNNGTC 1 cut(s) 235
EarI CTCTTC 3 cut(s) 91, 947, 1060
Eco130I CCWWGG 3 cut(s) 832, 941, 1094
Eco31I GGTCTC 1 cut(s) 132
Eco47I GGWCC 2 cut(s) 814, 825
Eco57I CTGAAG 1 cut(s) 126
EcoNI CCTNNNNNAGG 1 cut(s) 1150
EcoRII CCWGG 2 cut(s) 355, 734
EcoT14I CCWWGG 3 cut(s) 832, 941, 1094
ErhI CCWWGG 3 cut(s) 832, 941, 1094
FaqI GGGAC 1 cut(s) 716
FbaI TGATCA 1 cut(s) 678
Fnu4HI GCNGC 1 cut(s) 1182
FokI GGATG 3 cut(s) 167, 815, 905
Fsp4HI GCNGC 1 cut(s) 1182
FspBI CTAG 3 cut(s) 431, 566, 1178
GluI GCNGC 1 cut(s) 1182
GsaI CCCAGC 1 cut(s) 892
HindIII AAGCTT 1 cut(s) 779
HinfI GANTC 2 cut(s) 404, 667
HphI GGTGA 2 cut(s) 287, 983
Hpy188I TCNGA 6 cut(s) 187, 286, 414, 802, 1018, 1045
Hpy188III TCNNGA 8 cut(s) 322, 383, 401, 515, 566, 691, 752, 809
Hpy99I CGWCG 1 cut(s) 384
HpyAV CCTTC 2 cut(s) 150, 836
HpyCH4III ACNGT 1 cut(s) 615
HpyCH4IV ACGT 1 cut(s) 675
HpyCH4V TGCA 8 cut(s) 117, 193, 579, 597, 653, 851, 857, 922
HpyF10VI GCNNNNNNNGC 1 cut(s) 932
HpyF3I CTNAG 2 cut(s) 184, 285
HpySE526I ACGT 1 cut(s) 675
KpnI GGTACC 1 cut(s) 8
Ksp22I TGATCA 1 cut(s) 678
Kzo9I GATC 6 cut(s) 222, 678, 687, 1114, 1123, 1174
LmnI GCTCC 1 cut(s) 931
LweI GCATC 5 cut(s) 145, 424, 640, 860, 883
MaeI CTAG 3 cut(s) 431, 566, 1178
MaeII ACGT 1 cut(s) 675
MaeIII GTNAC 1 cut(s) 165
MalI GATC 6 cut(s) 224, 680, 689, 1116, 1125, 1176
MboI GATC 6 cut(s) 222, 678, 687, 1114, 1123, 1174
MboII GAAGA 5 cut(s) 108, 337, 676, 964, 1047
MflI RGATCY 3 cut(s) 687, 1114, 1174
MhlI GDGCHC 1 cut(s) 183
MluCI AATT 7 cut(s) 132, 332, 393, 489, 711, 976, 1107
MmeI TCCRAC 1 cut(s) 1083
MseI TTAA 1 cut(s) 419
MslI CAYNNNNRTG 2 cut(s) 598, 856
MspR9I CCNGG 2 cut(s) 357, 736
Mva1269I GAATGC 1 cut(s) 768
MvaI CCWGG 2 cut(s) 357, 736
MwoI GCNNNNNNNGC 1 cut(s) 932
NcoI CCATGG 1 cut(s) 832
NdeII GATC 6 cut(s) 222, 678, 687, 1114, 1123, 1174
NlaIV GGNNCC 4 cut(s) 6, 362, 377, 927
NspI RCATGY 6 cut(s) 67, 344, 579, 597, 702, 861
PaeI GCATGC 2 cut(s) 579, 597
PagI TCATGA 1 cut(s) 514
PciI ACATGT 3 cut(s) 63, 340, 698
PctI GAATGC 1 cut(s) 768
PfeI GAWTC 2 cut(s) 404, 667
PflFI GACNNNGTC 1 cut(s) 313
PkrI GCNGC 1 cut(s) 1183
PscI ACATGT 3 cut(s) 63, 340, 698
PsiI TTATAA 1 cut(s) 305
Psp6I CCWGG 2 cut(s) 355, 734
PspFI CCCAGC 1 cut(s) 888
PspGI CCWGG 2 cut(s) 355, 734
PspN4I GGNNCC 4 cut(s) 6, 362, 377, 927
PspPI GGNCC 2 cut(s) 814, 825
PsuI RGATCY 3 cut(s) 687, 1114, 1174
PsyI GACNNNGTC 1 cut(s) 313
RsaI GTAC 1 cut(s) 6
RsaNI GTAC 1 cut(s) 5
RseI CAYNNNNRTG 2 cut(s) 598, 856
SaqAI TTAA 1 cut(s) 419
SatI GCNGC 1 cut(s) 1182
Sau3AI GATC 6 cut(s) 222, 678, 687, 1114, 1123, 1174
Sau96I GGNCC 2 cut(s) 814, 825
ScrFI CCNGG 2 cut(s) 357, 736
SduI GDGCHC 1 cut(s) 183
SfaNI GCATC 5 cut(s) 145, 424, 640, 860, 883
SinI GGWCC 2 cut(s) 814, 825
SmiMI CAYNNNNRTG 2 cut(s) 598, 856
SmlI CTYRAG 2 cut(s) 28, 809
SmoI CTYRAG 2 cut(s) 28, 809
SphI GCATGC 2 cut(s) 579, 597
Sse9I AATT 7 cut(s) 132, 332, 393, 489, 711, 976, 1107
SspMI CTAG 3 cut(s) 431, 566, 1178
StyD4I CCNGG 2 cut(s) 355, 734
StyI CCWWGG 3 cut(s) 832, 941, 1094
TaaI ACNGT 1 cut(s) 615
TaiI ACGT 1 cut(s) 678
TaqI TCGA 1 cut(s) 648
TasI AATT 7 cut(s) 132, 332, 393, 489, 711, 976, 1107
TfiI GAWTC 2 cut(s) 404, 667
Tru1I TTAA 1 cut(s) 419
Tru9I TTAA 1 cut(s) 419
TscAI CASTG 1 cut(s) 202
TseI GCWGC 1 cut(s) 1181
TspDTI ATGAA 3 cut(s) 230, 671, 977
TspGWI ACGGA 3 cut(s) 229, 806, 964
TspRI CASTG 1 cut(s) 202
Tth111I GACNNNGTC 1 cut(s) 313
VpaK11BI GGWCC 2 cut(s) 814, 825
XagI CCTNNNNNAGG 1 cut(s) 1150
XapI RAATTY 3 cut(s) 132, 332, 393
XbaI TCTAGA 1 cut(s) 565
XceI RCATGY 6 cut(s) 67, 344, 579, 597, 702, 861
XspI CTAG 3 cut(s) 431, 566, 1178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.