Rh7DG000800

Calcineurin-like phosphoesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0003480
Physical Location & Seq
Forward (+)
113873 .. 119000
5128 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG000800.1

Sequence Viewer

Length: 816 bp
ATGCTCTGCATTTTCACTGTACACATGGTACCAAGTGTTGCCGTATCTTGCTTGAGCTTATCGCAATATGCTTATCATTCAAACCACATGTCAAAACATATATCATTGAGTAAATATAGAAGAGACATAAGGAGGGGTTGCATTACAAGGTCTCAAATTTTGCCTTCAGTTCTTGGGGATGCTGATGGGTTACGAGTATTTGTGCTCTCAGATTTGCACACTGACTATACTGAAAACATGAAATGGATCAAGGACTTATCGTCCGTCAGATACAAGAATGATGTTCTTATTGTTGCTGGTGATGTTTCTGAGACATACAAGAACTTTATAATGACCATGTCTCTCTTGAAGAATAAATTTGAACATGTCCTTTATGTTCCTGGGAACCATGACCTCTGGTGCCGTCGAGAGGGAGAAATTTATCTTGATTCTCATCAGAAGTTAAATAAAGTGCTAGATGCGTGTAGAGGACTTGGAGTTGAGACAAATCCAATAGTTTTAGATGGCTTGGGAATTATTCCTTTGCTCTCATGGTATCATGAGAGCTTTGATAAGGAGGAGGAAATAACCAATATCCACATTCCACCTCTAGAGATGGTAATTTGTGCTGTTTTTCATTTAACTATATTTGCCATAGAACGATTTTTTATGCTTCTGGCCAGTTCTGCTTTGGTCTTTAAGAATACCAGTTGTTCTTTCTCTCTATTGGTCCGCACAAATTTTACTTGTTCAACCCAATATGCTAGGGGAGAAGTGGACAACCGGTCCAGCCACCAGTTACTTTTCTTTTTTCCCCTCTCTTTCTCTCCCTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

271

Amino Acids

31.01

Weight (kDa)

6.85

Isoelectric Point (pI)

47.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 64 - 144 1.4e-10 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 329
Acc65I GGTACC 1 cut(s) 28
AccB1I GGYRCC 2 cut(s) 28, 399
AciI CCGC 1 cut(s) 712
AclWI GGATC 1 cut(s) 254
AcoI YGGCCR 1 cut(s) 657
AcsI RAATTY 4 cut(s) 156, 356, 417, 718
AcuI CTGAAG 1 cut(s) 150
AfaI GTAC 2 cut(s) 21, 30
AfiI CCNNNNNNNGG 1 cut(s) 409
AflIII ACRYGT 2 cut(s) 87, 364
AgeI ACCGGT 1 cut(s) 762
AgsI TTSAA 4 cut(s) 81, 349, 362, 732
AhdI GACNNNNNGTC 2 cut(s) 259, 763
AjnI CCWGG 1 cut(s) 379
AluBI AGCT 2 cut(s) 57, 546
AluI AGCT 2 cut(s) 57, 546
Alw21I GWGCWC 1 cut(s) 207
Alw26I GTCTC 5 cut(s) 117, 156, 305, 345, 476
AlwI GGATC 1 cut(s) 254
AoxI GGCC 1 cut(s) 657
ApoI RAATTY 4 cut(s) 156, 356, 417, 718
ArsI GACNNNNNNTTYG 2 cut(s) 74, 106
AsiGI ACCGGT 1 cut(s) 762
Asp718I GGTACC 1 cut(s) 28
AspS9I GGNCC 2 cut(s) 709, 765
AsuHPI GGTGA 1 cut(s) 311
AvaII GGWCC 2 cut(s) 709, 765
BalI TGGCCA 1 cut(s) 659
BanI GGYRCC 2 cut(s) 28, 399
Bbv12I GWGCWC 1 cut(s) 207
BccI CCATC 3 cut(s) 179, 497, 589
BceAI ACGGC 2 cut(s) 26, 387
BciT130I CCWGG 1 cut(s) 381
BcoDI GTCTC 5 cut(s) 117, 156, 305, 345, 476
BfaI CTAG 3 cut(s) 455, 590, 744
Bme1390I CCNGG 1 cut(s) 381
Bme18I GGWCC 2 cut(s) 709, 765
BmeRI GACNNNNNGTC 2 cut(s) 259, 763
BmgT120I GGNCC 2 cut(s) 709, 765
BmiI GGNNCC 3 cut(s) 30, 386, 401
BmrFI CCNGG 1 cut(s) 381
BmsI GCATC 2 cut(s) 169, 448
BpuEI CTTGAG 1 cut(s) 73
BsaBI GATNNNNATC 1 cut(s) 432
BsaI GGTCTC 1 cut(s) 156
BsaJI CCNNGG 1 cut(s) 380
BsaWI WCCGGW 1 cut(s) 762
Bsc4I CCNNNNNNNGG 1 cut(s) 409
Bse118I RCCGGY 1 cut(s) 762
Bse1I ACTGG 3 cut(s) 660, 687, 775
Bse8I GATNNNNATC 1 cut(s) 432
BseBI CCWGG 1 cut(s) 381
BseDI CCNNGG 1 cut(s) 380
BseGI GGATG 1 cut(s) 184
BseJI GATNNNNATC 1 cut(s) 432
BseLI CCNNNNNNNGG 1 cut(s) 409
BseMII CTCAG 2 cut(s) 222, 300
BseNI ACTGG 3 cut(s) 660, 687, 775
BseRI GAGGAG 1 cut(s) 572
BshFI GGCC 1 cut(s) 659
BshNI GGYRCC 2 cut(s) 28, 399
BshTI ACCGGT 1 cut(s) 762
BsiHKAI GWGCWC 1 cut(s) 207
BsiSI CCGG 1 cut(s) 763
BslI CCNNNNNNNGG 1 cut(s) 409
BsmAI GTCTC 5 cut(s) 117, 156, 305, 345, 476
BsnI GGCC 1 cut(s) 659
Bso31I GGTCTC 1 cut(s) 156
Bsp1286I GDGCHC 1 cut(s) 207
Bsp1407I TGTACA 1 cut(s) 19
Bsp143I GATC 1 cut(s) 246
BspACI CCGC 1 cut(s) 712
BspANI GGCC 1 cut(s) 659
BspCNI CTCAG 2 cut(s) 221, 301
BspHI TCATGA 1 cut(s) 538
BspLI GGNNCC 3 cut(s) 30, 386, 401
BspPI GGATC 1 cut(s) 254
BspT107I GGYRCC 2 cut(s) 28, 399
BspTNI GGTCTC 1 cut(s) 156
BsrFI RCCGGY 1 cut(s) 762
BsrGI TGTACA 1 cut(s) 19
BsrI ACTGG 3 cut(s) 660, 687, 775
BssAI RCCGGY 1 cut(s) 762
BssECI CCNNGG 1 cut(s) 380
BssMI GATC 1 cut(s) 246
Bst2UI CCWGG 1 cut(s) 381
Bst4CI ACNGT 1 cut(s) 19
Bst6I CTCTTC 1 cut(s) 115
BstAUI TGTACA 1 cut(s) 19
BstDEI CTNAG 2 cut(s) 208, 309
BstF5I GGATG 1 cut(s) 184
BstKTI GATC 1 cut(s) 249
BstMAI GTCTC 5 cut(s) 117, 156, 305, 345, 476
BstMBI GATC 1 cut(s) 246
BstMWI GCNNNNNNNGC 1 cut(s) 665
BstNI CCWGG 1 cut(s) 381
BstNSI RCATGY 2 cut(s) 91, 368
BstSCI CCNGG 1 cut(s) 379
BsuRI GGCC 1 cut(s) 659
BtsCI GGATG 1 cut(s) 184
BtsIMutI CAGTG 2 cut(s) 15, 219
CciI TCATGA 1 cut(s) 538
Cfr10I RCCGGY 1 cut(s) 762
Cfr13I GGNCC 2 cut(s) 709, 765
Csp6I GTAC 2 cut(s) 20, 29
CspAI ACCGGT 1 cut(s) 762
CviAII CATG 8 cut(s) 25, 88, 238, 337, 365, 389, 531, 539
CviJI RGCY 5 cut(s) 57, 507, 546, 659, 771
CviKI_1 RGCY 5 cut(s) 57, 507, 546, 659, 771
CviQI GTAC 2 cut(s) 20, 29
DdeI CTNAG 2 cut(s) 208, 309
DpnI GATC 1 cut(s) 248
DpnII GATC 1 cut(s) 246
DriI GACNNNNNGTC 2 cut(s) 259, 763
EaeI YGGCCR 1 cut(s) 657
Eam1104I CTCTTC 1 cut(s) 115
Eam1105I GACNNNNNGTC 2 cut(s) 259, 763
EarI CTCTTC 1 cut(s) 115
Eco31I GGTCTC 1 cut(s) 156
Eco47I GGWCC 2 cut(s) 709, 765
Eco57I CTGAAG 1 cut(s) 150
EcoRII CCWGG 1 cut(s) 379
FaeI CATG 8 cut(s) 28, 91, 241, 340, 368, 392, 534, 542
FatI CATG 8 cut(s) 24, 87, 237, 336, 364, 388, 530, 538
FokI GGATG 1 cut(s) 191
FspBI CTAG 3 cut(s) 455, 590, 744
HaeIII GGCC 1 cut(s) 659
HapII CCGG 1 cut(s) 763
Hin1II CATG 8 cut(s) 28, 91, 241, 340, 368, 392, 534, 542
HinfI GANTC 1 cut(s) 428
HpaII CCGG 1 cut(s) 763
HphI GGTGA 1 cut(s) 311
Hpy166II GTNNAC 2 cut(s) 22, 757
Hpy188I TCNGA 4 cut(s) 211, 269, 310, 438
Hpy188III TCNNGA 6 cut(s) 346, 407, 425, 539, 590, 813
Hpy8I GTNNAC 2 cut(s) 22, 757
Hpy99I CGWCG 1 cut(s) 408
HpyAV CCTTC 1 cut(s) 174
HpyCH4III ACNGT 1 cut(s) 19
HpyCH4V TGCA 3 cut(s) 9, 141, 217
HpyF10VI GCNNNNNNNGC 1 cut(s) 665
HpyF3I CTNAG 2 cut(s) 208, 309
Hsp92II CATG 8 cut(s) 28, 91, 241, 340, 368, 392, 534, 542
KpnI GGTACC 1 cut(s) 32
Kzo9I GATC 1 cut(s) 246
LweI GCATC 2 cut(s) 169, 448
MaeI CTAG 3 cut(s) 455, 590, 744
MaeIII GTNAC 2 cut(s) 189, 777
MalI GATC 1 cut(s) 248
MboI GATC 1 cut(s) 246
MboII GAAGA 2 cut(s) 132, 361
MhlI GDGCHC 1 cut(s) 207
MlsI TGGCCA 1 cut(s) 659
MluCI AATT 6 cut(s) 156, 356, 417, 513, 600, 718
MluNI TGGCCA 1 cut(s) 659
MnlI CCTC 8 cut(s) 126, 403, 404, 461, 550, 553, 597, 806
Mox20I TGGCCA 1 cut(s) 659
MscI TGGCCA 1 cut(s) 659
MseI TTAA 3 cut(s) 443, 620, 678
Msp20I TGGCCA 1 cut(s) 659
MspI CCGG 1 cut(s) 763
MspR9I CCNGG 1 cut(s) 381
MvaI CCWGG 1 cut(s) 381
MwoI GCNNNNNNNGC 1 cut(s) 665
NdeII GATC 1 cut(s) 246
NlaIII CATG 8 cut(s) 28, 91, 241, 340, 368, 392, 534, 542
NlaIV GGNNCC 3 cut(s) 30, 386, 401
NspI RCATGY 2 cut(s) 91, 368
PagI TCATGA 1 cut(s) 538
PciI ACATGT 2 cut(s) 87, 364
PfeI GAWTC 1 cut(s) 428
PflFI GACNNNGTC 1 cut(s) 337
PinAI ACCGGT 1 cut(s) 762
PscI ACATGT 2 cut(s) 87, 364
PsiI TTATAA 1 cut(s) 329
Psp6I CCWGG 1 cut(s) 379
PspGI CCWGG 1 cut(s) 379
PspN4I GGNNCC 3 cut(s) 30, 386, 401
PspPI GGNCC 2 cut(s) 709, 765
PsyI GACNNNGTC 1 cut(s) 337
RsaI GTAC 2 cut(s) 21, 30
RsaNI GTAC 2 cut(s) 20, 29
SaqAI TTAA 3 cut(s) 443, 620, 678
Sau3AI GATC 1 cut(s) 246
Sau96I GGNCC 2 cut(s) 709, 765
ScrFI CCNGG 1 cut(s) 381
SduI GDGCHC 1 cut(s) 207
SetI ASST 5 cut(s) 59, 152, 396, 548, 589
SfaNI GCATC 2 cut(s) 169, 448
SinI GGWCC 2 cut(s) 709, 765
SmlI CTYRAG 1 cut(s) 52
SmoI CTYRAG 1 cut(s) 52
Sse9I AATT 6 cut(s) 156, 356, 417, 513, 600, 718
SsiI CCGC 1 cut(s) 712
SspMI CTAG 3 cut(s) 455, 590, 744
StyD4I CCNGG 1 cut(s) 379
TaaI ACNGT 1 cut(s) 19
TaqI TCGA 1 cut(s) 406
TasI AATT 6 cut(s) 156, 356, 417, 513, 600, 718
TatI WGTACW 1 cut(s) 19
TfiI GAWTC 1 cut(s) 428
Tru1I TTAA 3 cut(s) 443, 620, 678
Tru9I TTAA 3 cut(s) 443, 620, 678
TscAI CASTG 2 cut(s) 22, 226
TspDTI ATGAA 2 cut(s) 254, 605
TspGWI ACGGA 1 cut(s) 253
TspRI CASTG 2 cut(s) 22, 226
Tth111I GACNNNGTC 1 cut(s) 337
VpaK11BI GGWCC 2 cut(s) 709, 765
XapI RAATTY 4 cut(s) 156, 356, 417, 718
XbaI TCTAGA 1 cut(s) 589
XceI RCATGY 2 cut(s) 91, 368
XcmI CCANNNNNNNNNTGG 1 cut(s) 667
XspI CTAG 3 cut(s) 455, 590, 744
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.