Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0007572

Physical Location & Seq

Genomic Coordinates Reverse (-)

1005561 .. 1013145

Gene Structure 7585 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1011100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3249 bp

ATGGAGTCAGAAGGAGAAACTGCGGCAAAGCCCATGAAGAGCATCGGGGGTAAGGTCTGCCAGATCTGTGGGGATAATGTTGGGAAGACTGCAGATGGGGAACCATTCATTGCCTGCGATGTCTGTGCCTTTCCTGTTTGCAGGCCATGCTATGAGTATGAGAGGAAGGATGGGAATCAGTCTTGCCCTCAATGCAAAACCAGATACAAGAGGCACAAAGGAAGCCCTGCAATTCTTGGTGATAGAGAAGAGGATGCTGTTGCTGATGATGATACCAGTGATTTCAACTATACTTCAGAAAATCAAAACGAGAAGCAAAAGATTGCAGAACGCATGCTAAGCTGGCATATGACATATGGCCGGGGAGAGGATGTTTCAGCCCCAAATTATGATAAAGAGGTTTCTCACAATCACATCCCTCTACTCGCTAGTGGCCAAGAGGTTTCCGGAGAACTGTCTGCCGCTTCACCAGAGCGCCTTTCGATGGCATCTCCTGGAGTTGGTCCTGGAAAGCGTGCCCATCATCATTCTTATGGATCAGATGTTAATCAATCCCCTAATATAAGGGTTGTGGATCCTGTGAGGGAGTTTGGTTCTCCCGGGATAGGAAATGTAGCATGGAAAGAGAGAGTGGATGGCTGGAAGATGAAGCAAGAGAAGAATGTCATTCCAATGAGCACTGGCCAAGCTACTTCAGAAAGAGGCGGTGGAGATATTGATGCCAGATCTGATGTGATTGTGGACGACTCCTTATTGAATGATGAAGCTAGGCAGCCTCTCTCCAGAAAGGTCTCGATTCCATCTTCTAGAATAAATCCTTACAGAATGGTCATTGTTCTGCGGCTTATTATTCTGTGCATTTTCTTGCACTATCGAATAACAAATCCTGTGCAAAATGCCTATGCTCTGTGGCTGATATCAGTCATTTGTGAGATTTGGTTTGCAATTTCCTGGATTCTTGATCAGTTCCCAAAGTGGCTCCCTGTAAATCGTGAAACATATCTTGACAGACTTTCTTTGAGATATGACCGAGAAGGAGAACCATCAAACTTAGCTGCTGTTGACATCTTTGTCAGTACTGTTGATCCATTGAAGGAGCCTCCTATGGTGACAGCAAATACTGTGCTATCTATTCTTGCAGTTGACTACCCGGTTGACAAGGTTTCTTGCTATGTTTCTGATGATGGAGCTGCAATGTTGACGTTTGAAGCTCTGTCTGAAACTTCAGAATTTGCTAGGAAATGGGTCCCTTTCTGCAAGAAGTATGCTATTGAGCCTCGGGCTCCTGAATGGTACTTTTCACAGAAGATTGATTACTTAAAGGATAAAGTTCAACCATCATTCGTCAAAGAGCGTAGAGCAATGAAGAGAGAATACGAAGAATTTAAAGTTCGTGTTAACGGGCTTGTTGCAAAGGCCACAAAAATACCCGAAGAAGGATGGATCATGCAAGATGGTACCCCATGGCCTGGAAATAATACTAGAGACCATCCAGGAATGATTCAGGTTTTCTTAGGCCAAAGTGGAGGGCTCGATACTGATGGTAATGAACTGCCACGTTTAGTCTATGTTTCTCGTGAAAAGCGTCCAGGTTTCCAACATCACAAGAAGGCTGGTGCTATGAATGCACTTGTTCGGGTATCTGCAGTCCTCACTAACGGACCTTTCCTGTTGAATCTTGATTGTGATCATTACATCAACAACAGTAAGGCCTTGAGGGAAGCTATGTGCTTTCTAATGGATCCTAACCTTGGGAAAAATGTGTGTTATGTTCAGTTCCCACAGAGGTTTGATGGTATTGATAGAAACGATCGATATGCCAATCGTAACACTGTTTTCTTTGATATTAACTTGAGGGGTTTAGATGGTATTCAAGGGCCAGTTTATGTGGGTACTGGATGTGTCTTCAATAGAACAGCTTTGTATGGTTACGAACCTCCTGTCAAACCTAAGCATAAGAAAACTGGAGTTCTATCTTCACTTTGTGGTGGATCACGGAAGAAGGGTTCAAAATCAAGTAAAAAGGGATCGGACAAAAAGAAATCAAATAAGAATGTCGATCCTACTGTGCCAATTTTCAGTCTAGAGGATATTGAAGAGGGGGTGGAAGGTGCTGGATTTGACGATGAGAAGTCATTATTGATGTCACAAATGAGCCTAGAAAAGAGGTTCGGCCAGTCTTCTGTTTTTGTTGCCTCTACACTGATGGAGAATGGCGGTGTTCCTCAATCTGCAACACCAGAAACTCTACTTAAAGAAGCTATTCATGTTATCAGCTGTGGATATGAAGACAAATCTGATTGGGGAAACGAGATTGGATGGATCTATGGTTCTGTCACAGAAGATATTCTTACAGGATTCAAGATGCATGCCCGCGGGTGGAGGTCTATATACTGCATGCCTAAGCTCCCAGCCTTTAAAGGGTCTGCTCCTATTAATCTTTCAGATCGTCTGAACCAAGTGCTTCGATGGGCTTTGGGATCTGTGGAAATTCTTCTTAGTCGGCATTGTCCTATCTGGTATGGTTACAGTGGGAGGCTAAAATGGCTGGAGAGATTTGCGTATGTGAACACTACCATTTACCCAATCACTTCCATACCTCTTCTCATGTACTGTACACTGCCGGCTGTCTGTCTTCTTACCAACAAATTTATCATCCCACAGATTAGCAACCTTGCGAGTATATGGTTTATCTCCCTCTTTCTTTCTATCTTTGCAACCGGTATTCTAGAGATGAGGTGGAGTGGTGTTGGAATCGATGAGTGGTGGAGAAATGAACAGTTTTGGGTTATTGGTGGTGTTTCTGCCCATCTGTTTGCGGTTGTCCAAGGTCTCCTCAAAGTACTTGCTGGTATTGACACCAACTTCACTGTCACCTCCAAGGCATCAGACGAAGACGGGGACTTTAATGAACTCTACATGTTTAAATGGACTACCCTTCTCATCCCACCAACGACTCTCCTCATCATAAACTTGGTGGGGGTTGTTGCAGGTATATCGTATGCCGTCAACAGTGGTTACCAATCATGGGGTCCCCTCTTTGGTAAGCTCTTCTTTGCCTTTTGGGTGATTGTCCATTTGTATCCATTCCTCAAGGGTCTCATGGGGCGCCAGAACCGTACGCCCACCATTGTAATCGTGTGGTCGATTCTCCTTGCTTCAATTTTCTCATTGTTGTGGGTGAGGGTTGATCCCTTTACCACGAGAGTCACCGGTCCAGATATTGAGGAGTGTGGAATCAACTGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0000278 GO:0000281 GO:0000910 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005886 GO:0005911 GO:0005975 GO:0005976 GO:0006073 GO:0006950 GO:0006952 GO:0007049 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009506 GO:0009832 GO:0009833 GO:0009834 GO:0009987 GO:0012505 GO:0016020 GO:0016051 GO:0016740 GO:0016757 GO:0016758 GO:0016759 GO:0022402 GO:0030054 GO:0030243 GO:0030244 GO:0031410 GO:0031982 GO:0031984 GO:0033692 GO:0034637 GO:0034645 GO:0042546 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046527 GO:0050896 GO:0051273 GO:0051274 GO:0051301 GO:0055044 GO:0061640 GO:0071554 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0097708 GO:0098791 GO:1901576 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cellulose_synt zf-UDP

Protein Analysis

1083

Amino Acids

121.16

Weight (kDa)

6.82

Isoelectric Point (pI)

38.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-UDP	PF14569	10 - 86	1.2e-40	Zinc-binding RING-finger
Cellulose_synt	PF03552	354 - 1075	0.0	Cellulose synthase
Glyco_trans_2_3	PF13632	771 - 876	9.6e-07	Glycosyl transferase family group 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0007572)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G05170
fragaria_vesca	FvH4_7g01100 FvH4_7g01100 FvH4_7g01100 FvH4_7g01100 FvH4_7g01100
malus_domestica	MD02G1311600.v1.1 MD07G1011100.v1.1
prunus_persica	Prupe.2G011200_v2.0.a1 Prupe.2G014300_v2.0.a1 Prupe.2G014300_v2.0.a1
pyrus_communis	pycom02g26140 pycom02g26150 pycom07g00920
rosa_chinensis	RchiOBHm_Chr1g0316091
rosa_laevigata	RLG00000030696
rosa_multiflora	Rmu_sc0002531.1_g000033
rosa_roxburghii	Rroxscaffold_4G00331140
rosa_rugosa	Rorug01G0008900
rosa_samantha	Rh1AG019000 Rh1BG013900 Rh1CG016600 Rh1DG013600
rosa_wichuraiana	Rw1G001230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1070
Acc36I	ACCTGC	1 cut(s)	2981
Acc65I	GGTACC	1 cut(s)	1457
AccB1I	GGYRCC	2 cut(s)	1457, 3108
AccB7I	CCANNNNNTGG	1 cut(s)	1469
AccII	CGCG	1 cut(s)	2376
AccIII	TCCGGA	1 cut(s)	446
AciI	CCGC	8 cut(s)	23, 462, 705, 841, 2217, 2374, 2376, 2819
AcoI	YGGCCR	4 cut(s)	358, 433, 682, 2173
AcsI	RAATTY	4 cut(s)	1229, 1382, 2490, 2648
AcuI	CTGAAG	3 cut(s)	279, 678, 1209
AcyI	GRCGYC	1 cut(s)	3109
AdeI	CACNNNGTG	1 cut(s)	1985
AfaI	GTAC	8 cut(s)	1078, 1295, 1459, 1894, 2612, 2617, 2844, 3121
AflIII	ACRYGT	1 cut(s)	2919
AgeI	ACCGGT	2 cut(s)	2720, 3212
AjnI	CCWGG	6 cut(s)	493, 505, 950, 1468, 1492, 1588
AloI	GAACNNNNNNTCC	4 cut(s)	1990, 2022, 2314, 2346
Alw21I	GWGCWC	1 cut(s)	680
Alw26I	GTCTC	4 cut(s)	796, 1479, 2837, 3104
Ama87I	CYCGRG	2 cut(s)	599, 1278
Aor13HI	TCCGGA	1 cut(s)	446
ApeKI	GCWGC	3 cut(s)	772, 1055, 1190
ApoI	RAATTY	4 cut(s)	1229, 1382, 2490, 2648
ArsI	GACNNNNNNTTYG	2 cut(s)	1926, 1958
AseI	ATTAAT	1 cut(s)	2436
AsiGI	ACCGGT	2 cut(s)	2720, 3212
Asp700I	GAANNNNTTC	3 cut(s)	2262, 2346, 2493
Asp718I	GGTACC	1 cut(s)	1457
AspLEI	GCGC	2 cut(s)	477, 3111
AspS9I	GGNCC	6 cut(s)	503, 1246, 1661, 1877, 3032, 3215
AsuC2I	CCSGG	4 cut(s)	362, 600, 601, 1151
AsuHPI	GGTGA	7 cut(s)	251, 459, 1120, 2866, 3079, 3193, 3202
AvaI	CYCGRG	2 cut(s)	599, 1278
AvaII	GGWCC	5 cut(s)	503, 1246, 1661, 3032, 3215
BaeGI	GKGCMC	1 cut(s)	520
BalI	TGGCCA	2 cut(s)	435, 684
BamHI	GGATCC	2 cut(s)	574, 1741
BanI	GGYRCC	2 cut(s)	1457, 3108
BanII	GRGCYC	2 cut(s)	1285, 1533
BarI	GAAGNNNNNNTAC	4 cut(s)	1298, 1330, 1358, 1390
BauI	CACGAG	2 cut(s)	1575, 3202
BbsI	GAAGAC	6 cut(s)	92, 1897, 2172, 2295, 2627, 2901
Bbv12I	GWGCWC	1 cut(s)	680
BbvI	GCAGC	3 cut(s)	784, 1042, 1177
BceAI	ACGGC	1 cut(s)	2990
BcgI	CGANNNNNNTGC	6 cut(s)	107, 141, 1799, 1833, 2979, 3013
BciT130I	CCWGG	6 cut(s)	495, 507, 952, 1470, 1494, 1590
BciVI	GTATCC	1 cut(s)	3093
BclI	TGATCA	2 cut(s)	961, 1687
BcnI	CCSGG	4 cut(s)	362, 600, 601, 1151
BcoDI	GTCTC	4 cut(s)	796, 1479, 2837, 3104
BfaI	CTAG	9 cut(s)	429, 768, 807, 1236, 1482, 2084, 2159, 2729, 3247
BfmI	CTRYAG	2 cut(s)	90, 1644
BfoI	RGCGCY	2 cut(s)	478, 3112
BfuAI	ACCTGC	1 cut(s)	2981
BfuI	GTATCC	1 cut(s)	3093
BglII	AGATCT	2 cut(s)	63, 725
BisI	GCNGC	6 cut(s)	24, 462, 773, 842, 1056, 1191
BlpI	GCTNAGC	1 cut(s)	338
BlsI	GCNGC	6 cut(s)	25, 463, 774, 843, 1057, 1192
BmcAI	AGTACT	2 cut(s)	1078, 2844
Bme18I	GGWCC	5 cut(s)	503, 1246, 1661, 3032, 3215
BmeT110I	CYCGRG	2 cut(s)	599, 1278
BmgT120I	GGNCC	6 cut(s)	503, 1246, 1661, 1877, 3032, 3215
BmsI	GCATC	6 cut(s)	51, 244, 497, 709, 2355, 2894
BpiI	GAAGAC	6 cut(s)	92, 1897, 2172, 2295, 2627, 2901
BpmI	CTGGAG	4 cut(s)	516, 766, 1986, 2570
Bpu10I	CCTNAGC	2 cut(s)	1950, 2403
Bpu1102I	GCTNAGC	1 cut(s)	338
BpuEI	CTTGAG	3 cut(s)	1735, 1873, 3077
BpuMI	CCSGG	4 cut(s)	362, 600, 601, 1151
Bsa29I	ATCGAT	2 cut(s)	1813, 2757
BsaBI	GATNNNNATC	3 cut(s)	174, 546, 1686
BsaHI	GRCGYC	1 cut(s)	3109
BsaI	GGTCTC	4 cut(s)	796, 1479, 2837, 3104
BsaJI	CCNNGG	8 cut(s)	361, 599, 1277, 1463, 1750, 2374, 2827, 2880
BsaWI	WCCGGW	3 cut(s)	446, 2720, 3212
BsaXI	ACNNNNNCTCC	2 cut(s)	357, 387
Bse118I	RCCGGY	3 cut(s)	2623, 2720, 3212
Bse1I	ACTGG	6 cut(s)	276, 685, 1880, 1900, 1969, 2176
Bse3DI	GCAATG	3 cut(s)	108, 1200, 1368
Bse8I	GATNNNNATC	3 cut(s)	174, 546, 1686
BseAI	TCCGGA	1 cut(s)	446
BseBI	CCWGG	6 cut(s)	495, 507, 952, 1470, 1494, 1590
BseCI	ATCGAT	2 cut(s)	1813, 2757
BseDI	CCNNGG	8 cut(s)	361, 599, 1277, 1463, 1750, 2374, 2827, 2880
BseJI	GATNNNNATC	3 cut(s)	174, 546, 1686
BseMI	GCAATG	3 cut(s)	108, 1200, 1368
BseNI	ACTGG	6 cut(s)	276, 685, 1880, 1900, 1969, 2176
BseRI	GAGGAG	3 cut(s)	2825, 2951, 3242
BseSI	GKGCMC	1 cut(s)	520
BseXI	GCAGC	3 cut(s)	784, 1042, 1177
BseYI	CCCAGC	1 cut(s)	2410
Bsh1236I	CGCG	1 cut(s)	2376
Bsh1285I	CGRYCG	1 cut(s)	1813
BshNI	GGYRCC	2 cut(s)	1457, 3108
BshTI	ACCGGT	2 cut(s)	2720, 3212
BshVI	ATCGAT	2 cut(s)	1813, 2757
BsiEI	CGRYCG	1 cut(s)	1813
BsiHKAI	GWGCWC	1 cut(s)	680
BsiHKCI	CYCGRG	2 cut(s)	599, 1278
BsiSI	CCGG	7 cut(s)	361, 447, 600, 1151, 2624, 2721, 3213
BsiWI	CGTACG	1 cut(s)	3119
BslFI	GGGAC	3 cut(s)	1232, 2915, 3018
BsmAI	GTCTC	4 cut(s)	796, 1479, 2837, 3104
BsmFI	GGGAC	3 cut(s)	1232, 2915, 3018
BsmI	GAATGC	1 cut(s)	1630
Bso31I	GGTCTC	4 cut(s)	796, 1479, 2837, 3104
BsoBI	CYCGRG	2 cut(s)	599, 1278
Bsp1286I	GDGCHC	4 cut(s)	520, 680, 1285, 1533
Bsp13I	TCCGGA	1 cut(s)	446
Bsp1407I	TGTACA	1 cut(s)	2615
Bsp1720I	GCTNAGC	1 cut(s)	338
Bsp19I	CCATGG	1 cut(s)	1463
BspACI	CCGC	8 cut(s)	23, 462, 705, 841, 2217, 2374, 2376, 2819
BspDI	ATCGAT	2 cut(s)	1813, 2757
BspEI	TCCGGA	1 cut(s)	446
BspFNI	CGCG	1 cut(s)	2376
BspMAI	CTGCAG	2 cut(s)	94, 1648
BspMI	ACCTGC	1 cut(s)	2981
BspQI	GCTCTTC	2 cut(s)	32, 3056
BspT107I	GGYRCC	2 cut(s)	1457, 3108
BspTNI	GGTCTC	4 cut(s)	796, 1479, 2837, 3104
BsrDI	GCAATG	3 cut(s)	108, 1200, 1368
BsrFI	RCCGGY	3 cut(s)	2623, 2720, 3212
BsrGI	TGTACA	1 cut(s)	2615
BsrI	ACTGG	6 cut(s)	276, 685, 1880, 1900, 1969, 2176
BssAI	RCCGGY	3 cut(s)	2623, 2720, 3212
BssECI	CCNNGG	8 cut(s)	361, 599, 1277, 1463, 1750, 2374, 2827, 2880
BssNI	GRCGYC	1 cut(s)	3109
BssSI	CACGAG	2 cut(s)	1575, 3202
BssT1I	CCWWGG	4 cut(s)	1463, 1750, 2827, 2880
Bst2BI	CACGAG	2 cut(s)	1575, 3202
Bst2UI	CCWGG	6 cut(s)	495, 507, 952, 1470, 1494, 1590
Bst6I	CTCTTC	6 cut(s)	32, 243, 1361, 2091, 2607, 3056
BstACI	GRCGYC	1 cut(s)	3109
BstAPI	GCANNNNNTGC	1 cut(s)	147
BstAUI	TGTACA	1 cut(s)	2615
BstC8I	GCNNGC	9 cut(s)	115, 143, 335, 344, 516, 2370, 2374, 2399, 2625
BstDEI	CTNAG	6 cut(s)	338, 1051, 1513, 1950, 2403, 2498
BstDSI	CCRYGG	2 cut(s)	1463, 2374
BstEII	GGTNACC	1 cut(s)	3017
BstFNI	CGCG	1 cut(s)	2376
BstH2I	RGCGCY	2 cut(s)	478, 3112
BstHHI	GCGC	2 cut(s)	477, 3111
BstMAI	GTCTC	4 cut(s)	796, 1479, 2837, 3104
BstMCI	CGRYCG	1 cut(s)	1813
BstMWI	GCNNNNNNNGC	6 cut(s)	147, 192, 339, 343, 1625, 2545
BstNI	CCWGG	6 cut(s)	495, 507, 952, 1470, 1494, 1590
BstNSI	RCATGY	4 cut(s)	337, 2372, 2401, 2923
BstPI	GGTNACC	1 cut(s)	3017
BstSFI	CTRYAG	2 cut(s)	90, 1644
BstSLI	GKGCMC	1 cut(s)	520
BstUI	CGCG	1 cut(s)	2376
BstV1I	GCAGC	3 cut(s)	784, 1042, 1177
BstV2I	GAAGAC	6 cut(s)	92, 1897, 2172, 2295, 2627, 2901
BstX2I	RGATCY	6 cut(s)	63, 574, 725, 1741, 2322, 2480
BstXI	CCANNNNNNTGG	1 cut(s)	68
BstYI	RGATCY	6 cut(s)	63, 574, 725, 1741, 2322, 2480
Bsu15I	ATCGAT	2 cut(s)	1813, 2757
BsuI	GTATCC	1 cut(s)	3093
BsuTUI	ATCGAT	2 cut(s)	1813, 2757
BtgI	CCRYGG	2 cut(s)	1463, 2374
BtgZI	GCGATG	1 cut(s)	132
BtsI	GCAGTG	1 cut(s)	2618
BtsIMutI	CAGTG	8 cut(s)	283, 678, 1830, 2201, 2536, 2618, 2868, 3019
BveI	ACCTGC	1 cut(s)	2981
Cac8I	GCNNGC	9 cut(s)	115, 143, 335, 344, 516, 2370, 2374, 2399, 2625
CfoI	GCGC	2 cut(s)	477, 3111
Cfr10I	RCCGGY	3 cut(s)	2623, 2720, 3212
Cfr13I	GGNCC	6 cut(s)	503, 1246, 1661, 1877, 3032, 3215
Cfr42I	CCGCGG	1 cut(s)	2377
Cfr9I	CCCGGG	1 cut(s)	599
ClaI	ATCGAT	2 cut(s)	1813, 2757
CseI	GACGC	1 cut(s)	1574
Csp6I	GTAC	8 cut(s)	1077, 1294, 1458, 1893, 2611, 2616, 2843, 3120
CspAI	ACCGGT	2 cut(s)	2720, 3212
CviQI	GTAC	8 cut(s)	1077, 1294, 1458, 1893, 2611, 2616, 2843, 3120
DdeI	CTNAG	6 cut(s)	338, 1051, 1513, 1950, 2403, 2498
DinI	GGCGCC	1 cut(s)	3110
DraI	TTTAAA	3 cut(s)	1387, 2419, 2926
DraIII	CACNNNGTG	1 cut(s)	1985
DrdI	GACNNNNNNGTC	1 cut(s)	1070
DseDI	GACNNNNNNGTC	1 cut(s)	1070
EaeI	YGGCCR	4 cut(s)	358, 433, 682, 2173
Eam1104I	CTCTTC	6 cut(s)	32, 243, 1361, 2091, 2607, 3056
EarI	CTCTTC	6 cut(s)	32, 243, 1361, 2091, 2607, 3056
Eco130I	CCWWGG	4 cut(s)	1463, 1750, 2827, 2880
Eco147I	AGGCCT	1 cut(s)	1712
Eco24I	GRGCYC	2 cut(s)	1285, 1533
Eco31I	GGTCTC	4 cut(s)	796, 1479, 2837, 3104
Eco32I	GATATC	1 cut(s)	918
Eco47I	GGWCC	5 cut(s)	503, 1246, 1661, 3032, 3215
Eco57I	CTGAAG	3 cut(s)	279, 678, 1209
Eco88I	CYCGRG	2 cut(s)	599, 1278
Eco91I	GGTNACC	1 cut(s)	3017
EcoO109I	RGGNCCY	2 cut(s)	1246, 3032
EcoO65I	GGTNACC	1 cut(s)	3017
EcoRII	CCWGG	6 cut(s)	493, 505, 950, 1468, 1492, 1588
EcoRV	GATATC	1 cut(s)	918
EcoT14I	CCWWGG	4 cut(s)	1463, 1750, 2827, 2880
EcoT22I	ATGCAT	1 cut(s)	2370
EcoT38I	GRGCYC	2 cut(s)	1285, 1533
EgeI	GGCGCC	1 cut(s)	3110
EheI	GGCGCC	1 cut(s)	3110
ErhI	CCWWGG	4 cut(s)	1463, 1750, 2827, 2880
FalI	AAGNNNNNCTT	4 cut(s)	2334, 2366, 3038, 3070
FaqI	GGGAC	3 cut(s)	1232, 2915, 3018
FauI	CCCGC	2 cut(s)	2369, 2381
FauNDI	CATATG	2 cut(s)	348, 355
FbaI	TGATCA	2 cut(s)	961, 1687
Fnu4HI	GCNGC	6 cut(s)	24, 462, 773, 842, 1056, 1191
FriOI	GRGCYC	2 cut(s)	1285, 1533
Fsp4HI	GCNGC	6 cut(s)	24, 462, 773, 842, 1056, 1191
FspBI	CTAG	9 cut(s)	429, 768, 807, 1236, 1482, 2084, 2159, 2729, 3247
GlaI	GCGC	2 cut(s)	476, 3110
GluI	GCNGC	6 cut(s)	24, 462, 773, 842, 1056, 1191
GsaI	CCCAGC	1 cut(s)	2414
GsuI	CTGGAG	4 cut(s)	516, 766, 1986, 2570
HaeII	RGCGCY	2 cut(s)	478, 3112
HapII	CCGG	7 cut(s)	361, 447, 600, 1151, 2624, 2721, 3213
HgaI	GACGC	1 cut(s)	1574
HhaI	GCGC	2 cut(s)	477, 3111
Hin1I	GRCGYC	1 cut(s)	3109
Hin6I	GCGC	2 cut(s)	475, 3109
HinP1I	GCGC	2 cut(s)	475, 3109
HincII	GTYRAC	6 cut(s)	1063, 1144, 1156, 1200, 1399, 3010
HindII	GTYRAC	6 cut(s)	1063, 1144, 1156, 1200, 1399, 3010
HpaI	GTTAAC	1 cut(s)	1399
HpaII	CCGG	7 cut(s)	361, 447, 600, 1151, 2624, 2721, 3213
HphI	GGTGA	7 cut(s)	251, 459, 1120, 2866, 3079, 3193, 3202
Hpy166II	GTNNAC	9 cut(s)	742, 1063, 1144, 1156, 1200, 1399, 2569, 2618, 3010
Hpy8I	GTNNAC	9 cut(s)	742, 1063, 1144, 1156, 1200, 1399, 2569, 2618, 3010
HpyAV	CCTTC	9 cut(s)	5, 160, 1028, 1087, 1430, 1603, 1996, 2102, 2948
HpyCH4IV	ACGT	2 cut(s)	1202, 1558
HpyF10VI	GCNNNNNNNGC	6 cut(s)	147, 192, 339, 343, 1625, 2545
HpyF3I	CTNAG	6 cut(s)	338, 1051, 1513, 1950, 2403, 2498
HpySE526I	ACGT	2 cut(s)	1202, 1558
Hsp92I	GRCGYC	1 cut(s)	3109
HspAI	GCGC	2 cut(s)	475, 3109
KasI	GGCGCC	1 cut(s)	3108
KflI	GGGWCCC	2 cut(s)	1246, 3032
Kpn2I	TCCGGA	1 cut(s)	446
KpnI	GGTACC	1 cut(s)	1461
KroI	GCCGGC	1 cut(s)	2623
KroNI	GCCGGC	1 cut(s)	2625
Ksp22I	TGATCA	2 cut(s)	961, 1687
KspAI	GTTAAC	1 cut(s)	1399
KspI	CCGCGG	1 cut(s)	2377
LguI	GCTCTTC	2 cut(s)	32, 3056
LmnI	GCTCC	6 cut(s)	984, 1096, 1187, 1288, 2412, 2434
Lsp1109I	GCAGC	3 cut(s)	784, 1042, 1177
LweI	GCATC	6 cut(s)	51, 244, 497, 709, 2355, 2894
MaeI	CTAG	9 cut(s)	429, 768, 807, 1236, 1482, 2084, 2159, 2729, 3247
MaeII	ACGT	2 cut(s)	1202, 1558
MaeIII	GTNAC	9 cut(s)	1108, 1826, 1928, 2145, 2335, 2525, 2872, 3017, 3208
MflI	RGATCY	6 cut(s)	63, 574, 725, 1741, 2322, 2480
MhlI	GDGCHC	4 cut(s)	520, 680, 1285, 1533
MlsI	TGGCCA	2 cut(s)	435, 684
MluCI	AATT	9 cut(s)	231, 385, 945, 1229, 1382, 2073, 2490, 2648, 3162
MluNI	TGGCCA	2 cut(s)	435, 684
Mly113I	GGCGCC	1 cut(s)	3109
MlyI	GAGTC	4 cut(s)	14, 740, 2950, 3216
MmeI	TCCRAC	2 cut(s)	1621, 2731
Mox20I	TGGCCA	2 cut(s)	435, 684
Mph1103I	ATGCAT	1 cut(s)	2370
MroI	TCCGGA	1 cut(s)	446
MroNI	GCCGGC	1 cut(s)	2623
MroXI	GAANNNNTTC	3 cut(s)	2262, 2346, 2493
MscI	TGGCCA	2 cut(s)	435, 684
MslI	CAYNNNNRTG	3 cut(s)	531, 671, 3175
Msp20I	TGGCCA	2 cut(s)	435, 684
MspA1I	CMGCKG	2 cut(s)	2277, 2376
MspI	CCGG	7 cut(s)	361, 447, 600, 1151, 2624, 2721, 3213
Mva1269I	GAATGC	1 cut(s)	1630
MvaI	CCWGG	6 cut(s)	495, 507, 952, 1470, 1494, 1590
MvnI	CGCG	1 cut(s)	2376
MwoI	GCNNNNNNNGC	6 cut(s)	147, 192, 339, 343, 1625, 2545
NaeI	GCCGGC	1 cut(s)	2625
NarI	GGCGCC	1 cut(s)	3109
NciI	CCSGG	4 cut(s)	362, 600, 601, 1151
NcoI	CCATGG	1 cut(s)	1463
NdeI	CATATG	2 cut(s)	348, 355
NgoMIV	GCCGGC	1 cut(s)	2623
NmuCI	GTSAC	5 cut(s)	1108, 2145, 2335, 2872, 3208
NsiI	ATGCAT	1 cut(s)	2370
NspI	RCATGY	4 cut(s)	337, 2372, 2401, 2923
PaeI	GCATGC	3 cut(s)	337, 2372, 2401
PceI	AGGCCT	1 cut(s)	1712
PciI	ACATGT	1 cut(s)	2919
PciSI	GCTCTTC	2 cut(s)	32, 3056
PcsI	WCGNNNNNNNCGW	1 cut(s)	1582
PctI	GAATGC	1 cut(s)	1630
PdiI	GCCGGC	1 cut(s)	2625
PdmI	GAANNNNTTC	3 cut(s)	2262, 2346, 2493
PfeI	GAWTC	9 cut(s)	175, 796, 955, 1501, 1675, 2358, 2754, 3148, 3237
Pfl23II	CGTACG	1 cut(s)	3119
PflMI	CCANNNNNTGG	1 cut(s)	1469
PfoI	TCCNGGA	4 cut(s)	493, 505, 950, 1492
PinAI	ACCGGT	2 cut(s)	2720, 3212
PkrI	GCNGC	6 cut(s)	25, 463, 774, 843, 1057, 1192
Ple19I	CGATCG	1 cut(s)	1813
PleI	GAGTC	4 cut(s)	13, 740, 2950, 3215
PluTI	GGCGCC	1 cut(s)	3112
PpsI	GAGTC	4 cut(s)	13, 740, 2950, 3215
PpuMI	RGGWCCY	2 cut(s)	1246, 3032
PscI	ACATGT	1 cut(s)	2919
PshBI	ATTAAT	1 cut(s)	2436
Psp5II	RGGWCCY	2 cut(s)	1246, 3032
Psp6I	CCWGG	6 cut(s)	493, 505, 950, 1468, 1492, 1588
PspEI	GGTNACC	1 cut(s)	3017
PspFI	CCCAGC	1 cut(s)	2410
PspGI	CCWGG	6 cut(s)	493, 505, 950, 1468, 1492, 1588
PspLI	CGTACG	1 cut(s)	3119
PspPI	GGNCC	6 cut(s)	503, 1246, 1661, 1877, 3032, 3215
PspPPI	RGGWCCY	2 cut(s)	1246, 3032
PstI	CTGCAG	2 cut(s)	94, 1648
PsuI	RGATCY	6 cut(s)	63, 574, 725, 1741, 2322, 2480
PvuI	CGATCG	1 cut(s)	1813
PvuII	CAGCTG	1 cut(s)	2277
RsaI	GTAC	8 cut(s)	1078, 1295, 1459, 1894, 2612, 2617, 2844, 3121
RsaNI	GTAC	8 cut(s)	1077, 1294, 1458, 1893, 2611, 2616, 2843, 3120
RseI	CAYNNNNRTG	3 cut(s)	531, 671, 3175
SacII	CCGCGG	1 cut(s)	2377
SapI	GCTCTTC	2 cut(s)	32, 3056
SatI	GCNGC	6 cut(s)	24, 462, 773, 842, 1056, 1191
Sau96I	GGNCC	6 cut(s)	503, 1246, 1661, 1877, 3032, 3215
ScaI	AGTACT	2 cut(s)	1078, 2844
SchI	GAGTC	4 cut(s)	14, 740, 2950, 3216
SduI	GDGCHC	4 cut(s)	520, 680, 1285, 1533
SfaNI	GCATC	6 cut(s)	51, 244, 497, 709, 2355, 2894
SfcI	CTRYAG	2 cut(s)	90, 1644
SfoI	GGCGCC	1 cut(s)	3110
Sfr303I	CCGCGG	1 cut(s)	2377
SgrBI	CCGCGG	1 cut(s)	2377
SinI	GGWCC	5 cut(s)	503, 1246, 1661, 3032, 3215
SmaI	CCCGGG	1 cut(s)	601
SmiMI	CAYNNNNRTG	3 cut(s)	531, 671, 3175
SmlI	CTYRAG	3 cut(s)	1714, 1852, 3092
SmoI	CTYRAG	3 cut(s)	1714, 1852, 3092
SphI	GCATGC	3 cut(s)	337, 2372, 2401
Sse9I	AATT	9 cut(s)	231, 385, 945, 1229, 1382, 2073, 2490, 2648, 3162
SseBI	AGGCCT	1 cut(s)	1712
SsiI	CCGC	8 cut(s)	23, 462, 705, 841, 2217, 2374, 2376, 2819
SspDI	GGCGCC	1 cut(s)	3108
SspMI	CTAG	9 cut(s)	429, 768, 807, 1236, 1482, 2084, 2159, 2729, 3247
StuI	AGGCCT	1 cut(s)	1712
StyI	CCWWGG	4 cut(s)	1463, 1750, 2827, 2880
TaiI	ACGT	2 cut(s)	1205, 1561
TaqI	TCGA	9 cut(s)	482, 794, 874, 1533, 1813, 2058, 2467, 2757, 3146
TaqII	GACCGA	1 cut(s)	1044
TasI	AATT	9 cut(s)	231, 385, 945, 1229, 1382, 2073, 2490, 2648, 3162
TatI	WGTACW	4 cut(s)	1076, 2610, 2615, 2842
TauI	GCSGC	3 cut(s)	26, 464, 844
TfiI	GAWTC	9 cut(s)	175, 796, 955, 1501, 1675, 2358, 2754, 3148, 3237
TscAI	CASTG	8 cut(s)	283, 685, 1837, 2208, 2536, 2625, 2875, 3019
TseFI	GTSAC	5 cut(s)	1108, 2145, 2335, 2872, 3208
TseI	GCWGC	3 cut(s)	772, 1055, 1190
Tsp45I	GTSAC	5 cut(s)	1108, 2145, 2335, 2872, 3208
TspGWI	ACGGA	2 cut(s)	1674, 2011
TspMI	CCCGGG	1 cut(s)	599
TspRI	CASTG	8 cut(s)	283, 685, 1837, 2208, 2536, 2625, 2875, 3019
Van91I	CCANNNNNTGG	1 cut(s)	1469
VpaK11BI	GGWCC	5 cut(s)	503, 1246, 1661, 3032, 3215
VspI	ATTAAT	1 cut(s)	2436
XapI	RAATTY	4 cut(s)	1229, 1382, 2490, 2648
XbaI	TCTAGA	3 cut(s)	806, 2083, 2728
XceI	RCATGY	4 cut(s)	337, 2372, 2401, 2923
XmaI	CCCGGG	1 cut(s)	599
XmnI	GAANNNNTTC	3 cut(s)	2262, 2346, 2493
XspI	CTAG	9 cut(s)	429, 768, 807, 1236, 1482, 2084, 2159, 2729, 3247
ZrmI	AGTACT	2 cut(s)	1078, 2844
Zsp2I	ATGCAT	1 cut(s)	2370

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.