Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0007572

Physical Location & Seq

Genomic Coordinates Forward (+)

66057153 .. 66063387

Gene Structure 6235 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000030696

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3249 bp

ATGGAGTCGGAAGGAGAAACTGAGGCAAAGCCGATGAAGAACTTGGGTGGTCAGGTCTGCCAGATCTGTGGGGATAATGTTGGGAAGACAGCTGCTGGTGAACCATTCATTGCCTGCGATGTCTGCTCTTTTCCTGTTTGCAGGCCATGCTATGAGTATGAGAGGAAGGATGGAAATCAGTCTTGCCCTCAATGCAAAACCAGATACAAGAGGCACAAAGGAAGTCCTGCAATTCTTGGTGATAGAGAAGAGGACGCTGATGCTGATGACGGTGCTAGTGATTTCAACTATACTTCAGAAAATCAAAACGAGAAACAAAAGATTGCCGAGCGCATGTTGAGCTGGCATATGACATATGGGCGAGGAGAGGATATGGGGGGTCCAAACTATGATAAAGAGGTTTCTCACAATCACATCCCTCTGCTCACTAATGGAACAGAGGTTTCTGGAGAACTGTCTGCTGCCTCACCAGGGCGCCTTTCCATGGCATCTCCTGGAGCTTCAATTGGTGGAAAGCGTGTCCATCCTCTTCCTTATGCATCGGATGTTAATCAATCACCTAATTTAAGGGTTGTGGATCCTGTGAGAGAGTTTGGTTCTCCCGGGATAGGTAATGTAGCGTGGAAAGAGAGAGTGGATGGCTGGAAGATGAAGCAGGAGAAGAATACTATTCCAATGAGCACTGGTCAAGCTGCTTCAGAAAGAGGGGGTGGAGATATTGATGCCAGCACTGATGTGATTGTGGATGACTCTTTGAATGATGAAGCAAGGCAACCTCTCTCTAGAAAGGTCTCTATTCCATCGTCTAGGATAAATCCTTACAGGATGGTCATTGTACTGCGGCTGGTTATTCTTTGTATTTTCTTGCACTATCGGTTAACAAATCCTGTGCGCAATGCCTATGCCCTGTGGTTGATATCAGTCATTTGTGAGATTTGGTTCGCAATATCTTGGATTCTCGATCAGTTCCCTAAGTGGCTGCCTGTAAATCGTGAGACATATCTCGACAGACTTTCTCTGAGATATGACCGAGAAGGAGAACCATCACAGTTAGCTGCTGTTGACATCTTCGTCAGTACTGTGGATCCACTGAAGGAGCCTCCTATGGTGACAGCAAATACTGTGCTATCTATTCTTTCTGTTGACTATCCAGTTGACAAGGTTTCTTGCTATGTTTCTGATGATGGAGCTGCAATGTTGACATTTGAAGCTCTCTCTGAAACTTCAGAATTTGCAAGGAAATGGGTCCCTTTCTGCAAGAAGTATGCCATAGAGCCTAGGGCTCCTGAATGGTACTTTTCACAGAAAATTGATTATTTGAAGGATAAAGTTCAACCATCATTTGTCAAGGACCGCAGAGCAATGAAGAGAGAATACGAAGAATTTAAAGTTCGTGTGAATGGGCTTGTTGCAAAGGCGACAAAAGTACCTGAAGAAGGATGGATCATGCAAGACGGTACTCCATGGCCTGGAAATAATACTAGAGACCATCCAGGAATGATTCAGGTTTTCTTGGGGCAAAGTGGAGGGCTTGATGCTGAGGGCAATGAACTGCCACGTTTAGTGTATGTTTCTCGTGAAAAGCGTCCAGGTTTCCAACATCACAAGAAGGCTGGTGCTATGAATGCACTTGTTCGAGTATCTGCAGTCCTCACGAATGGACCCTTCTTGTTGAATCTTGATTGTGATCACTACATTAACAATAGTAAGGCCTTGAGGGAAGCAATGTGCTTCCTAATGGATCCTAACCTTGGAAAAAACGTGTGCTATGTTCAGTTTCCACAGAGATTTGATGGCATTGATAGAAATGATCGTTATGCCAATCGAAATACTGTTTTCTTTGATATTAACTTGAGAGGTTTAGATGGTATCCAAGGCCCAGTTTATGTGGGTACTGGATGTGTTTTCAATAGAACAGCCTTGTATGGTTATGAACCTCCTCTCAAGGTTAAGAATAAGAAAGCTGGAGTTCTTTCTTCACTTTGTGGTGGATCACGAAAGAAGGGTTCAAAATCAGGTAAAAAAGGCTCAGACAAGAAGAAATCAAGTAAGCATGTGGATCCTACTGTGCCGATTTTCAGTCTAGAGGATATTGAAGAGGGGGTTGAAGGTACTGGATTTGATGATGAGAAGTCATTATTGATGTCACAAATGAGCCTGGAGAAAAGATTTGGTCAGTCTGCTGTTTTTGTTGCTTCGACACTTATGGAGAATGGTGGTGTTCCTCAATCTGCGACACCCGAAACTCTTCTTAAAGAAGCTATTCATGTTATTAGCTGTGGGTATGAAGACAAAAGTGACTGGGGACAAGAGATTGGATGGATTTATGGTTCTGTCACAGAGGATATTCTTACAGGATTCAAGATGCATGCCCGCGGGTGGAGGTCTATTTACTGCATGCCTAAGCGCCCAGCCTTTAAAGGGTCTGCTCCCATTAATCTTTCAGATCGTCTCAATCAAGTGCTTCGATGGGCGTTGGGTTCTGTGGAAATTCTTCTTAGTCGGCATTGTCCTATCTGGTATGGCTATAGTGGTAGGCTAAAATGGCTGGAGAGATTTGCATATGTGAACACTACCATTTATCCAATTACTGCCATACCACTTCTTGCGTACTGTACATTGCCGGCCGTTTGTCTTCTTACCAACAAATTCATCATTCCGCAGATTAGCAACATTGCAAGTATATGGTTCATCTCCCTCTTTCTTTCCATCTTTGCAACTGGTATTCTAGAGATGAGATGGAGTGGTGTTGGAATTGACGAGTGGTGGAGAAATGAACAATTTTGGGTTATTGGTGGTGTTTCAGCCCATCTTTTTGCTGTCGTCCAGGGTCTCCTGAAAGTACTTGCAGGTATTGACACCAACTTTACTGTCACCTCCAAGGCGTCTGACGAAGACGGAGACTTTGCTGAACTCTACATGTTTAAATGGACAACCCTTCTCATCCCGCCAACAACTCTCCTCATCATAAACTTGGTGGGGGTGGTTGCCGGTATATCGTATGCCGTCAACAGTGGTTACCAGTCATGGGGTCCCCTCTTTGGTAAGCTCTTCTTTGCCTTCTGGGTGATCGTCCATTTGTATCCCTTCCTCAAAGGTCTTATGGGACGTCAGAACCGTACGCCTACAATTGTTGTCGTGTGGTCAATTCTCCTGGCTTCAATTTTCTCTTTGTTGTGGGTGAGGGTTGATCCATTTACCACAAGAGTCACTGGTCCAGATGTTGAGGTGTGTGGAATCAACTGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000271 GO:0000278 GO:0000281 GO:0000910 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005886 GO:0005911 GO:0005975 GO:0005976 GO:0006073 GO:0006950 GO:0006952 GO:0007049 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009250 GO:0009506 GO:0009832 GO:0009833 GO:0009834 GO:0009987 GO:0012505 GO:0016020 GO:0016051 GO:0016740 GO:0016757 GO:0016758 GO:0016759 GO:0022402 GO:0030054 GO:0030243 GO:0030244 GO:0031410 GO:0031982 GO:0031984 GO:0033692 GO:0034637 GO:0034645 GO:0042546 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044042 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044262 GO:0044264 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0046527 GO:0050896 GO:0051273 GO:0051274 GO:0051301 GO:0055044 GO:0061640 GO:0071554 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:0097708 GO:0098791 GO:1901576 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cellulose_synt zf-UDP

Protein Analysis

1083

Amino Acids

120.78

Weight (kDa)

7.18

Isoelectric Point (pI)

37.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-UDP	PF14569	10 - 86	3.4e-40	Zinc-binding RING-finger
Cellulose_synt	PF03552	354 - 1076	0.0	Cellulose synthase
Glyco_trans_2_3	PF13632	770 - 876	2.6e-07	Glycosyl transferase family group 2

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0007572)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G05170
fragaria_vesca	FvH4_7g01100 FvH4_7g01100 FvH4_7g01100 FvH4_7g01100 FvH4_7g01100
malus_domestica	MD02G1311600.v1.1 MD07G1011100.v1.1
prunus_persica	Prupe.2G011200_v2.0.a1 Prupe.2G014300_v2.0.a1 Prupe.2G014300_v2.0.a1
pyrus_communis	pycom02g26140 pycom02g26150 pycom07g00920
rosa_chinensis	RchiOBHm_Chr1g0316091
rosa_laevigata	RLG00000030696
rosa_multiflora	Rmu_sc0002531.1_g000033
rosa_roxburghii	Rroxscaffold_4G00331140
rosa_rugosa	Rorug01G0008900
rosa_samantha	Rh1AG019000 Rh1BG013900 Rh1CG016600 Rh1DG013600
rosa_wichuraiana	Rw1G001230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1070
AatII	GACGTC	1 cut(s)	3112
Acc16I	TGCGCA	1 cut(s)	893
Acc36I	ACCTGC	1 cut(s)	2840
AccB1I	GGYRCC	1 cut(s)	474
AccB7I	CCANNNNNTGG	1 cut(s)	1469
AccII	CGCG	1 cut(s)	2376
AciI	CCGC	6 cut(s)	841, 1354, 2374, 2376, 2660, 2948
AcoI	YGGCCR	1 cut(s)	2625
AcsI	RAATTY	4 cut(s)	1229, 1382, 2490, 2648
AcuI	CTGAAG	5 cut(s)	279, 681, 1112, 1209, 1452
AcyI	GRCGYC	3 cut(s)	475, 2885, 3109
AdeI	CACNNNGTG	1 cut(s)	1985
AflIII	ACRYGT	2 cut(s)	1761, 2919
AjnI	CCWGG	8 cut(s)	469, 493, 1468, 1492, 1588, 2157, 2826, 3153
AleI	CACNNNNGTG	1 cut(s)	734
AloI	GAACNNNNNNTCC	2 cut(s)	2314, 2346
Alw21I	GWGCWC	1 cut(s)	683
Alw26I	GTCTC	6 cut(s)	796, 989, 1479, 2456, 2837, 2895
AlwNI	CAGNNNCTG	1 cut(s)	95
Ama87I	CYCGRG	1 cut(s)	602
AoxI	GGCC	5 cut(s)	143, 1466, 1710, 1876, 2625
ApeKI	GCWGC	6 cut(s)	92, 461, 692, 979, 1055, 1190
ApoI	RAATTY	4 cut(s)	1229, 1382, 2490, 2648
ArsI	GACNNNNNNTTYG	2 cut(s)	2888, 2920
AseI	ATTAAT	1 cut(s)	2436
Asp700I	GAANNNNTTC	3 cut(s)	2247, 2262, 2493
AspA2I	CCTAGG	1 cut(s)	1277
AspLEI	GCGC	4 cut(s)	333, 477, 894, 2409
AspS9I	GGNCC	7 cut(s)	380, 1246, 1351, 1661, 1877, 3032, 3215
AsuC2I	CCSGG	2 cut(s)	603, 604
AsuHPI	GGTGA	8 cut(s)	110, 251, 459, 549, 1120, 2866, 3079, 3193
AvaI	CYCGRG	1 cut(s)	602
AvaII	GGWCC	6 cut(s)	380, 1246, 1351, 1661, 3032, 3215
AvrII	CCTAGG	1 cut(s)	1277
BaeI	ACNNNNGTAYC	2 cut(s)	1410, 1443
BamHI	GGATCC	4 cut(s)	577, 1084, 1741, 2059
BanI	GGYRCC	1 cut(s)	474
BanII	GRGCYC	1 cut(s)	1285
BarI	GAAGNNNNNNTAC	2 cut(s)	1358, 1390
BauI	CACGAG	1 cut(s)	1575
BbsI	GAAGAC	4 cut(s)	92, 2295, 2627, 2901
Bbv12I	GWGCWC	1 cut(s)	683
BbvCI	CCTCAGC	1 cut(s)	1539
BbvI	GCAGC	6 cut(s)	79, 448, 679, 966, 1042, 1177
BceAI	ACGGC	2 cut(s)	2612, 2990
BciT130I	CCWGG	8 cut(s)	471, 495, 1470, 1494, 1590, 2159, 2828, 3155
BciVI	GTATCC	2 cut(s)	1880, 3093
BclI	TGATCA	1 cut(s)	1687
BcnI	CCSGG	2 cut(s)	603, 604
BcoDI	GTCTC	6 cut(s)	796, 989, 1479, 2456, 2837, 2895
BfaI	CTAG	8 cut(s)	276, 783, 807, 1278, 1482, 2084, 2729, 3247
BfmI	CTRYAG	2 cut(s)	1644, 2527
BfoI	RGCGCY	2 cut(s)	478, 2410
BfuAI	ACCTGC	1 cut(s)	2840
BfuI	GTATCC	2 cut(s)	1880, 3093
BglII	AGATCT	1 cut(s)	63
BisI	GCNGC	7 cut(s)	93, 462, 693, 842, 980, 1056, 1191
BlnI	CCTAGG	1 cut(s)	1277
BlsI	GCNGC	7 cut(s)	94, 463, 694, 843, 981, 1057, 1192
BmcAI	AGTACT	2 cut(s)	1078, 2844
Bme18I	GGWCC	6 cut(s)	380, 1246, 1351, 1661, 3032, 3215
BmeT110I	CYCGRG	1 cut(s)	602
BmgT120I	GGNCC	7 cut(s)	380, 1246, 1351, 1661, 1877, 3032, 3215
BmrI	ACTGGG	2 cut(s)	1874, 2311
BmsI	GCATC	6 cut(s)	250, 497, 548, 712, 1525, 2355
BmuI	ACTGGG	2 cut(s)	1874, 2311
BpiI	GAAGAC	4 cut(s)	92, 2295, 2627, 2901
BpmI	CTGGAG	5 cut(s)	468, 516, 1986, 2180, 2570
Bpu10I	CCTNAGC	2 cut(s)	1539, 2403
BpuEI	CTTGAG	3 cut(s)	1735, 1873, 1928
BpuMI	CCSGG	2 cut(s)	603, 604
BsaBI	GATNNNNATC	3 cut(s)	174, 549, 1686
BsaHI	GRCGYC	3 cut(s)	475, 2885, 3109
BsaI	GGTCTC	3 cut(s)	796, 1479, 2837
Bse118I	RCCGGY	2 cut(s)	2623, 2990
Bse1I	ACTGG	9 cut(s)	688, 1151, 1880, 1900, 2119, 2306, 2725, 3022, 3217
Bse3DI	GCAATG	8 cut(s)	108, 901, 1200, 1368, 1552, 1731, 2618, 2673
Bse8I	GATNNNNATC	3 cut(s)	174, 549, 1686
BseBI	CCWGG	8 cut(s)	471, 495, 1470, 1494, 1590, 2159, 2828, 3155
BseJI	GATNNNNATC	3 cut(s)	174, 549, 1686
BseMI	GCAATG	8 cut(s)	108, 901, 1200, 1368, 1552, 1731, 2618, 2673
BseMII	CTCAG	4 cut(s)	12, 1010, 1530, 2043
BseNI	ACTGG	9 cut(s)	688, 1151, 1880, 1900, 2119, 2306, 2725, 3022, 3217
BseRI	GAGGAG	3 cut(s)	378, 1929, 2951
BseX3I	CGGCCG	1 cut(s)	2625
BseXI	GCAGC	6 cut(s)	79, 448, 679, 966, 1042, 1177
BseYI	CCCAGC	1 cut(s)	2410
Bsh1236I	CGCG	1 cut(s)	2376
Bsh1285I	CGRYCG	1 cut(s)	2628
BshFI	GGCC	5 cut(s)	145, 1468, 1712, 1878, 2627
BshNI	GGYRCC	1 cut(s)	474
BsiEI	CGRYCG	1 cut(s)	2628
BsiHKAI	GWGCWC	1 cut(s)	683
BsiHKCI	CYCGRG	1 cut(s)	602
BsiSI	CCGG	3 cut(s)	603, 2624, 2991
BsiWI	CGTACG	1 cut(s)	3119
BslFI	GGGAC	4 cut(s)	1232, 2319, 3018, 3120
BsmAI	GTCTC	6 cut(s)	796, 989, 1479, 2456, 2837, 2895
BsmBI	CGTCTC	1 cut(s)	2456
BsmFI	GGGAC	4 cut(s)	1232, 2319, 3018, 3120
BsmI	GAATGC	1 cut(s)	1630
BsnI	GGCC	5 cut(s)	145, 1468, 1712, 1878, 2627
Bso31I	GGTCTC	3 cut(s)	796, 1479, 2837
BsoBI	CYCGRG	1 cut(s)	602
Bsp1286I	GDGCHC	2 cut(s)	683, 1285
Bsp1407I	TGTACA	1 cut(s)	2615
Bsp19I	CCATGG	2 cut(s)	483, 1463
BspACI	CCGC	6 cut(s)	841, 1354, 2374, 2376, 2660, 2948
BspANI	GGCC	5 cut(s)	145, 1468, 1712, 1878, 2627
BspCNI	CTCAG	4 cut(s)	13, 1011, 1531, 2042
BspFNI	CGCG	1 cut(s)	2376
BspMAI	CTGCAG	1 cut(s)	1648
BspMI	ACCTGC	1 cut(s)	2840
BspQI	GCTCTTC	1 cut(s)	3056
BspT107I	GGYRCC	1 cut(s)	474
BspTNI	GGTCTC	3 cut(s)	796, 1479, 2837
BsrDI	GCAATG	8 cut(s)	108, 901, 1200, 1368, 1552, 1731, 2618, 2673
BsrFI	RCCGGY	2 cut(s)	2623, 2990
BsrGI	TGTACA	1 cut(s)	2615
BsrI	ACTGG	9 cut(s)	688, 1151, 1880, 1900, 2119, 2306, 2725, 3022, 3217
BssAI	RCCGGY	2 cut(s)	2623, 2990
BssNI	GRCGYC	3 cut(s)	475, 2885, 3109
BssSI	CACGAG	1 cut(s)	1575
BssT1I	CCWWGG	6 cut(s)	483, 1277, 1463, 1750, 1873, 2880
Bst2BI	CACGAG	1 cut(s)	1575
Bst2UI	CCWGG	8 cut(s)	471, 495, 1470, 1494, 1590, 2159, 2828, 3155
Bst6I	CTCTTC	6 cut(s)	243, 534, 1361, 2091, 2253, 3056
BstACI	GRCGYC	3 cut(s)	475, 2885, 3109
BstAPI	GCANNNNNTGC	1 cut(s)	147
BstAUI	TGTACA	1 cut(s)	2615
BstC8I	GCNNGC	8 cut(s)	115, 143, 344, 727, 2370, 2374, 2399, 2625
BstDEI	CTNAG	7 cut(s)	21, 972, 1019, 1539, 2029, 2403, 2498
BstDSI	CCRYGG	3 cut(s)	483, 1463, 2374
BstEII	GGTNACC	1 cut(s)	3017
BstENI	CCTNNNNNAGG	1 cut(s)	1434
BstFNI	CGCG	1 cut(s)	2376
BstH2I	RGCGCY	2 cut(s)	478, 2410
BstHHI	GCGC	4 cut(s)	333, 477, 894, 2409
BstMAI	GTCTC	6 cut(s)	796, 989, 1479, 2456, 2837, 2895
BstMCI	CGRYCG	1 cut(s)	2628
BstMWI	GCNNNNNNNGC	6 cut(s)	123, 147, 192, 339, 1625, 2545
BstNI	CCWGG	8 cut(s)	471, 495, 1470, 1494, 1590, 2159, 2828, 3155
BstNSI	RCATGY	5 cut(s)	337, 2057, 2372, 2401, 2923
BstPI	GGTNACC	1 cut(s)	3017
BstSFI	CTRYAG	2 cut(s)	1644, 2527
BstUI	CGCG	1 cut(s)	2376
BstV1I	GCAGC	6 cut(s)	79, 448, 679, 966, 1042, 1177
BstV2I	GAAGAC	4 cut(s)	92, 2295, 2627, 2901
BstX2I	RGATCY	5 cut(s)	63, 577, 1084, 1741, 2059
BstXI	CCANNNNNNTGG	1 cut(s)	68
BstYI	RGATCY	5 cut(s)	63, 577, 1084, 1741, 2059
BstZI	CGGCCG	1 cut(s)	2625
BsuI	GTATCC	2 cut(s)	1880, 3093
BsuRI	GGCC	5 cut(s)	145, 1468, 1712, 1878, 2627
BtgI	CCRYGG	3 cut(s)	483, 1463, 2374
BtgZI	GCGATG	1 cut(s)	132
BtsIMutI	CAGTG	5 cut(s)	681, 729, 1088, 3019, 3210
BveI	ACCTGC	1 cut(s)	2840
Cac8I	GCNNGC	8 cut(s)	115, 143, 344, 727, 2370, 2374, 2399, 2625
CaiI	CAGNNNCTG	1 cut(s)	95
CfoI	GCGC	4 cut(s)	333, 477, 894, 2409
Cfr10I	RCCGGY	2 cut(s)	2623, 2990
Cfr13I	GGNCC	7 cut(s)	380, 1246, 1351, 1661, 1877, 3032, 3215
Cfr42I	CCGCGG	1 cut(s)	2377
Cfr9I	CCCGGG	1 cut(s)	602
CseI	GACGC	3 cut(s)	263, 1574, 2874
DdeI	CTNAG	7 cut(s)	21, 972, 1019, 1539, 2029, 2403, 2498
DinI	GGCGCC	1 cut(s)	476
DraI	TTTAAA	3 cut(s)	1387, 2419, 2926
DraIII	CACNNNGTG	1 cut(s)	1985
DrdI	GACNNNNNNGTC	1 cut(s)	1070
DseDI	GACNNNNNNGTC	1 cut(s)	1070
EaeI	YGGCCR	1 cut(s)	2625
EagI	CGGCCG	1 cut(s)	2625
Eam1104I	CTCTTC	6 cut(s)	243, 534, 1361, 2091, 2253, 3056
EarI	CTCTTC	6 cut(s)	243, 534, 1361, 2091, 2253, 3056
EclXI	CGGCCG	1 cut(s)	2625
Eco130I	CCWWGG	6 cut(s)	483, 1277, 1463, 1750, 1873, 2880
Eco147I	AGGCCT	1 cut(s)	1712
Eco24I	GRGCYC	1 cut(s)	1285
Eco31I	GGTCTC	3 cut(s)	796, 1479, 2837
Eco32I	GATATC	1 cut(s)	918
Eco47I	GGWCC	6 cut(s)	380, 1246, 1351, 1661, 3032, 3215
Eco52I	CGGCCG	1 cut(s)	2625
Eco57I	CTGAAG	5 cut(s)	279, 681, 1112, 1209, 1452
Eco88I	CYCGRG	1 cut(s)	602
Eco91I	GGTNACC	1 cut(s)	3017
EcoNI	CCTNNNNNAGG	1 cut(s)	1434
EcoO109I	RGGNCCY	2 cut(s)	1246, 3032
EcoO65I	GGTNACC	1 cut(s)	3017
EcoRII	CCWGG	8 cut(s)	469, 493, 1468, 1492, 1588, 2157, 2826, 3153
EcoRV	GATATC	1 cut(s)	918
EcoT14I	CCWWGG	6 cut(s)	483, 1277, 1463, 1750, 1873, 2880
EcoT22I	ATGCAT	2 cut(s)	541, 2370
EcoT38I	GRGCYC	1 cut(s)	1285
EgeI	GGCGCC	1 cut(s)	476
EheI	GGCGCC	1 cut(s)	476
ErhI	CCWWGG	6 cut(s)	483, 1277, 1463, 1750, 1873, 2880
Esp3I	CGTCTC	1 cut(s)	2456
FalI	AAGNNNNNCTT	2 cut(s)	3038, 3070
FaqI	GGGAC	4 cut(s)	1232, 2319, 3018, 3120
FauI	CCCGC	3 cut(s)	2369, 2381, 2955
FauNDI	CATATG	3 cut(s)	348, 355, 2563
FbaI	TGATCA	1 cut(s)	1687
Fnu4HI	GCNGC	7 cut(s)	93, 462, 693, 842, 980, 1056, 1191
FriOI	GRGCYC	1 cut(s)	1285
Fsp4HI	GCNGC	7 cut(s)	93, 462, 693, 842, 980, 1056, 1191
FspBI	CTAG	8 cut(s)	276, 783, 807, 1278, 1482, 2084, 2729, 3247
FspI	TGCGCA	1 cut(s)	893
GlaI	GCGC	4 cut(s)	332, 476, 893, 2408
GluI	GCNGC	7 cut(s)	93, 462, 693, 842, 980, 1056, 1191
GsaI	CCCAGC	1 cut(s)	2414
GsuI	CTGGAG	5 cut(s)	468, 516, 1986, 2180, 2570
HaeII	RGCGCY	2 cut(s)	478, 2410
HaeIII	GGCC	5 cut(s)	145, 1468, 1712, 1878, 2627
HapII	CCGG	3 cut(s)	603, 2624, 2991
HgaI	GACGC	3 cut(s)	263, 1574, 2874
HhaI	GCGC	4 cut(s)	333, 477, 894, 2409
Hin1I	GRCGYC	3 cut(s)	475, 2885, 3109
Hin6I	GCGC	4 cut(s)	331, 475, 892, 2407
HinP1I	GCGC	4 cut(s)	331, 475, 892, 2407
HincII	GTYRAC	6 cut(s)	879, 1063, 1144, 1156, 1200, 3010
HindII	GTYRAC	6 cut(s)	879, 1063, 1144, 1156, 1200, 3010
HinfI	GANTC	8 cut(s)	5, 749, 955, 1501, 1675, 2358, 3207, 3237
HpaI	GTTAAC	1 cut(s)	879
HpaII	CCGG	3 cut(s)	603, 2624, 2991
HphI	GGTGA	8 cut(s)	110, 251, 459, 549, 1120, 2866, 3079, 3193
Hpy166II	GTNNAC	8 cut(s)	101, 879, 1063, 1144, 1156, 1200, 2569, 3010
Hpy8I	GTNNAC	8 cut(s)	101, 879, 1063, 1144, 1156, 1200, 2569, 3010
HpyCH4IV	ACGT	3 cut(s)	1558, 1761, 3109
HpyF10VI	GCNNNNNNNGC	6 cut(s)	123, 147, 192, 339, 1625, 2545
HpyF3I	CTNAG	7 cut(s)	21, 972, 1019, 1539, 2029, 2403, 2498
HpySE526I	ACGT	3 cut(s)	1558, 1761, 3109
Hsp92I	GRCGYC	3 cut(s)	475, 2885, 3109
HspAI	GCGC	4 cut(s)	331, 475, 892, 2407
KasI	GGCGCC	1 cut(s)	474
KflI	GGGWCCC	2 cut(s)	1246, 3032
KroI	GCCGGC	1 cut(s)	2623
KroNI	GCCGGC	1 cut(s)	2625
Ksp22I	TGATCA	1 cut(s)	1687
KspAI	GTTAAC	1 cut(s)	879
KspI	CCGCGG	1 cut(s)	2377
LguI	GCTCTTC	1 cut(s)	3056
LmnI	GCTCC	5 cut(s)	497, 1096, 1187, 1288, 2434
Lsp1109I	GCAGC	6 cut(s)	79, 448, 679, 966, 1042, 1177
LweI	GCATC	6 cut(s)	250, 497, 548, 712, 1525, 2355
MaeI	CTAG	8 cut(s)	276, 783, 807, 1278, 1482, 2084, 2729, 3247
MaeII	ACGT	3 cut(s)	1558, 1761, 3109
MaeIII	GTNAC	7 cut(s)	1108, 2145, 2297, 2335, 2872, 3017, 3208
MfeI	CAATTG	2 cut(s)	504, 3129
MflI	RGATCY	5 cut(s)	63, 577, 1084, 1741, 2059
MhlI	GDGCHC	2 cut(s)	683, 1285
Mly113I	GGCGCC	1 cut(s)	475
MlyI	GAGTC	3 cut(s)	14, 743, 3216
MmeI	TCCRAC	2 cut(s)	1621, 2731
Mph1103I	ATGCAT	2 cut(s)	541, 2370
MroNI	GCCGGC	1 cut(s)	2623
MroXI	GAANNNNTTC	3 cut(s)	2247, 2262, 2493
MslI	CAYNNNNRTG	1 cut(s)	734
MspA1I	CMGCKG	2 cut(s)	92, 2376
MspI	CCGG	3 cut(s)	603, 2624, 2991
MunI	CAATTG	2 cut(s)	504, 3129
Mva1269I	GAATGC	1 cut(s)	1630
MvaI	CCWGG	8 cut(s)	471, 495, 1470, 1494, 1590, 2159, 2828, 3155
MvnI	CGCG	1 cut(s)	2376
MwoI	GCNNNNNNNGC	6 cut(s)	123, 147, 192, 339, 1625, 2545
NaeI	GCCGGC	1 cut(s)	2625
NarI	GGCGCC	1 cut(s)	475
NciI	CCSGG	2 cut(s)	603, 604
NcoI	CCATGG	2 cut(s)	483, 1463
NdeI	CATATG	3 cut(s)	348, 355, 2563
NgoMIV	GCCGGC	1 cut(s)	2623
NmeAIII	GCCGAG	1 cut(s)	352
NmuCI	GTSAC	6 cut(s)	1108, 2145, 2297, 2335, 2872, 3208
NsbI	TGCGCA	1 cut(s)	893
NsiI	ATGCAT	2 cut(s)	541, 2370
NspI	RCATGY	5 cut(s)	337, 2057, 2372, 2401, 2923
OliI	CACNNNNGTG	1 cut(s)	734
PaeI	GCATGC	2 cut(s)	2372, 2401
PceI	AGGCCT	1 cut(s)	1712
PciI	ACATGT	1 cut(s)	2919
PciSI	GCTCTTC	1 cut(s)	3056
PcsI	WCGNNNNNNNCGW	2 cut(s)	1582, 3115
PctI	GAATGC	1 cut(s)	1630
PdiI	GCCGGC	1 cut(s)	2625
PdmI	GAANNNNTTC	3 cut(s)	2247, 2262, 2493
PfeI	GAWTC	5 cut(s)	955, 1501, 1675, 2358, 3237
Pfl23II	CGTACG	1 cut(s)	3119
PflMI	CCANNNNNTGG	1 cut(s)	1469
PfoI	TCCNGGA	2 cut(s)	493, 1492
PkrI	GCNGC	7 cut(s)	94, 463, 694, 843, 981, 1057, 1192
PleI	GAGTC	3 cut(s)	13, 743, 3215
PluTI	GGCGCC	1 cut(s)	478
PpsI	GAGTC	3 cut(s)	13, 743, 3215
PpuMI	RGGWCCY	2 cut(s)	1246, 3032
PscI	ACATGT	1 cut(s)	2919
PshBI	ATTAAT	1 cut(s)	2436
Psp5II	RGGWCCY	2 cut(s)	1246, 3032
Psp6I	CCWGG	8 cut(s)	469, 493, 1468, 1492, 1588, 2157, 2826, 3153
PspEI	GGTNACC	1 cut(s)	3017
PspFI	CCCAGC	1 cut(s)	2410
PspGI	CCWGG	8 cut(s)	469, 493, 1468, 1492, 1588, 2157, 2826, 3153
PspLI	CGTACG	1 cut(s)	3119
PspPI	GGNCC	7 cut(s)	380, 1246, 1351, 1661, 1877, 3032, 3215
PspPPI	RGGWCCY	2 cut(s)	1246, 3032
PstI	CTGCAG	1 cut(s)	1648
PstNI	CAGNNNCTG	1 cut(s)	95
PsuI	RGATCY	5 cut(s)	63, 577, 1084, 1741, 2059
PvuII	CAGCTG	1 cut(s)	92
RseI	CAYNNNNRTG	1 cut(s)	734
SacII	CCGCGG	1 cut(s)	2377
SapI	GCTCTTC	1 cut(s)	3056
SatI	GCNGC	7 cut(s)	93, 462, 693, 842, 980, 1056, 1191
Sau96I	GGNCC	7 cut(s)	380, 1246, 1351, 1661, 1877, 3032, 3215
ScaI	AGTACT	2 cut(s)	1078, 2844
SchI	GAGTC	3 cut(s)	14, 743, 3216
SduI	GDGCHC	2 cut(s)	683, 1285
SfaNI	GCATC	6 cut(s)	250, 497, 548, 712, 1525, 2355
SfcI	CTRYAG	2 cut(s)	1644, 2527
SfoI	GGCGCC	1 cut(s)	476
Sfr303I	CCGCGG	1 cut(s)	2377
SgrBI	CCGCGG	1 cut(s)	2377
SinI	GGWCC	6 cut(s)	380, 1246, 1351, 1661, 3032, 3215
SmaI	CCCGGG	1 cut(s)	604
SmiMI	CAYNNNNRTG	1 cut(s)	734
SmlI	CTYRAG	3 cut(s)	1714, 1852, 1943
SmoI	CTYRAG	3 cut(s)	1714, 1852, 1943
SphI	GCATGC	2 cut(s)	2372, 2401
SseBI	AGGCCT	1 cut(s)	1712
SsiI	CCGC	6 cut(s)	841, 1354, 2374, 2376, 2660, 2948
SspDI	GGCGCC	1 cut(s)	474
SspMI	CTAG	8 cut(s)	276, 783, 807, 1278, 1482, 2084, 2729, 3247
StuI	AGGCCT	1 cut(s)	1712
StyI	CCWWGG	6 cut(s)	483, 1277, 1463, 1750, 1873, 2880
TaiI	ACGT	3 cut(s)	1561, 1764, 3112
TaqI	TCGA	6 cut(s)	960, 1005, 1636, 1825, 2198, 2467
TaqII	GACCGA	1 cut(s)	1044
TatI	WGTACW	4 cut(s)	835, 1076, 2615, 2842
TauI	GCSGC	1 cut(s)	844
TfiI	GAWTC	5 cut(s)	955, 1501, 1675, 2358, 3237
TscAI	CASTG	5 cut(s)	688, 736, 1095, 3019, 3217
TseFI	GTSAC	6 cut(s)	1108, 2145, 2297, 2335, 2872, 3208
TseI	GCWGC	6 cut(s)	92, 461, 692, 979, 1055, 1190
Tsp45I	GTSAC	6 cut(s)	1108, 2145, 2297, 2335, 2872, 3208
TspGWI	ACGGA	1 cut(s)	2913
TspMI	CCCGGG	1 cut(s)	602
TspRI	CASTG	5 cut(s)	688, 736, 1095, 3019, 3217
Van91I	CCANNNNNTGG	1 cut(s)	1469
VpaK11BI	GGWCC	6 cut(s)	380, 1246, 1351, 1661, 3032, 3215
VspI	ATTAAT	1 cut(s)	2436
XagI	CCTNNNNNAGG	1 cut(s)	1434
XapI	RAATTY	4 cut(s)	1229, 1382, 2490, 2648
XbaI	TCTAGA	3 cut(s)	782, 2083, 2728
XceI	RCATGY	5 cut(s)	337, 2057, 2372, 2401, 2923
XmaI	CCCGGG	1 cut(s)	602
XmaJI	CCTAGG	1 cut(s)	1277
XmnI	GAANNNNTTC	3 cut(s)	2247, 2262, 2493
XspI	CTAG	8 cut(s)	276, 783, 807, 1278, 1482, 2084, 2729, 3247
ZraI	GACGTC	1 cut(s)	3110
ZrmI	AGTACT	2 cut(s)	1078, 2844
Zsp2I	ATGCAT	2 cut(s)	541, 2370

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.