Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0016383

Physical Location & Seq

Genomic Coordinates Forward (+)

6475590 .. 6480822

Gene Structure 5233 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1068500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 657 bp

ATGATATCTTCAGGGATAAACCTGGTGATGACGGTGATTGGGTTCGCGGTGAGCACCATGTTCATCGTGTTCGTGTGTACCAGGCTTGTCTGTGCTCGGATTCACCTCAGTGTATCTAGACGCTCCTTCCCCATAGCTTCCGGATCTGATCTCAATATTCTGGAACGGGGATTGCATGGCGTTGAACCTGTAGTAATAGCCAACTTTCCAACAAAGAAGTACAGTGATGCATTTTTTTCAGCTGTAGAAGATGCTCAATGTACTGTTTGCCTTGCGGAATACCATGGTGATGATGTATTGCGGATCCTCCCCTACTGTGAACACTCCTTCCATGTGACCTGTATAGACATATGGCTTCGGCAGCATTCCACATGTCCTGTTTGTAGAATATCATTGCGCGAGTTTCCTGAGATAAAACGCCGCATGCAACCCTTGTCCTGTTCGGCTATTCGATCTCATTACGGTACAGAGTCCTTTAATACCCTTTCTTATCGCTATCTCTTGAATAGTCGAGCATCAAGAACTCATGAAAACCATGGTATGGACCCCATTCAAGAAGATCATGCAGCATCAGAGGGTGATGCAACAGATGCCGGGGAGAATAGCTCCTCCTTAACTGAGAGTAATCAGATTTCTAATCAGATTTCTAAGGACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

219

Amino Acids

24.33

Weight (kDa)

6.04

Isoelectric Point (pI)

50.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	86 - 129	1.7e-13	Ring finger domain
zf-rbx1	PF12678	86 - 129	4.1e-09	RING-H2 zinc finger domain
zf-RING_11	PF17123	86 - 115	6.9e-08	RING-like zinc finger
zf-C3HC4_2	PF13923	86 - 128	9.7e-07	Zinc finger, C3HC4 type (RING finger)
zf-ANAPC11	PF12861	86 - 131	1.9e-06	Anaphase-promoting complex subunit 11 RING-H2 finger
zf-C3HC4	PF00097	87 - 128	6.1e-06	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016383)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g05950
malus_domestica	MD02G1247900.v1.1 MD07G1068500.v1.1
prunus_persica	Prupe.2G086200_v2.0.a1
pyrus_communis	pycom02g21200 pycom07g05270
rosa_chinensis	RchiOBHm_Chr1g0333171
rosa_laevigata	RLG00000029621
rosa_roxburghii	Rroxscaffold_4G00317720
rosa_rugosa	Rorug01G0107900
rosa_samantha	Rh1AG130900 Rh1BG100100 Rh1CG124700 Rh1DG136300
rosa_wichuraiana	Rw0G011810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	541
AccII	CGCG	2 cut(s)	47, 399
AccIII	TCCGGA	1 cut(s)	140
AciI	CCGC	4 cut(s)	47, 275, 301, 421
AclWI	GGATC	3 cut(s)	151, 298, 311
AfaI	GTAC	4 cut(s)	79, 221, 262, 466
AfiI	CCNNNNNNNGG	1 cut(s)	541
AflIII	ACRYGT	1 cut(s)	371
AgsI	TTSAA	3 cut(s)	185, 505, 554
AjnI	CCWGG	2 cut(s)	21, 80
AleI	CACNNNNGTG	1 cut(s)	108
AloI	GAACNNNNNNTCC	2 cut(s)	312, 344
AluBI	AGCT	3 cut(s)	137, 242, 606
AluI	AGCT	3 cut(s)	137, 242, 606
Alw21I	GWGCWC	2 cut(s)	56, 97
AlwI	GGATC	3 cut(s)	151, 298, 311
Aor13HI	TCCGGA	1 cut(s)	140
ApeKI	GCWGC	2 cut(s)	361, 566
AspLEI	GCGC	1 cut(s)	399
AspS9I	GGNCC	1 cut(s)	544
AsuC2I	CCSGG	1 cut(s)	595
AsuHPI	GGTGA	6 cut(s)	37, 46, 61, 95, 299, 590
AvaII	GGWCC	1 cut(s)	544
BamHI	GGATCC	1 cut(s)	303
Bbv12I	GWGCWC	2 cut(s)	56, 97
BbvI	GCAGC	2 cut(s)	373, 578
BciT130I	CCWGG	2 cut(s)	23, 82
BcnI	CCSGG	1 cut(s)	595
BfaI	CTAG	2 cut(s)	117, 655
BfmI	CTRYAG	2 cut(s)	189, 243
BisI	GCNGC	3 cut(s)	362, 421, 567
BlsI	GCNGC	3 cut(s)	363, 422, 568
Bme1390I	CCNGG	3 cut(s)	23, 82, 595
Bme18I	GGWCC	1 cut(s)	544
BmgT120I	GGNCC	1 cut(s)	544
BmiI	GGNNCC	2 cut(s)	305, 546
BmrFI	CCNGG	3 cut(s)	23, 82, 595
BmsI	GCATC	6 cut(s)	217, 241, 524, 571, 578, 580
BplI	GAGNNNNNCTC	2 cut(s)	590, 622
BpuMI	CCSGG	1 cut(s)	595
BsaJI	CCNNGG	3 cut(s)	283, 535, 594
BsaWI	WCCGGW	1 cut(s)	140
Bsc4I	CCNNNNNNNGG	1 cut(s)	541
Bse3DI	GCAATG	1 cut(s)	392
BseAI	TCCGGA	1 cut(s)	140
BseBI	CCWGG	2 cut(s)	23, 82
BseDI	CCNNGG	3 cut(s)	283, 535, 594
BseLI	CCNNNNNNNGG	1 cut(s)	541
BseMI	GCAATG	1 cut(s)	392
BseMII	CTCAG	3 cut(s)	121, 399, 609
BseRI	GAGGAG	1 cut(s)	598
BseXI	GCAGC	2 cut(s)	373, 578
Bsh1236I	CGCG	2 cut(s)	47, 399
BsiHKAI	GWGCWC	2 cut(s)	56, 97
BsiSI	CCGG	2 cut(s)	141, 594
BslI	CCNNNNNNNGG	1 cut(s)	541
BsmI	GAATGC	1 cut(s)	364
Bsp1286I	GDGCHC	2 cut(s)	56, 97
Bsp13I	TCCGGA	1 cut(s)	140
Bsp143I	GATC	5 cut(s)	143, 148, 303, 452, 559
Bsp19I	CCATGG	2 cut(s)	283, 535
BspACI	CCGC	4 cut(s)	47, 275, 301, 421
BspCNI	CTCAG	3 cut(s)	120, 400, 610
BspEI	TCCGGA	1 cut(s)	140
BspFNI	CGCG	2 cut(s)	47, 399
BspHI	TCATGA	1 cut(s)	526
BspLI	GGNNCC	2 cut(s)	305, 546
BspPI	GGATC	3 cut(s)	151, 298, 311
BsrDI	GCAATG	1 cut(s)	392
BssECI	CCNNGG	3 cut(s)	283, 535, 594
BssMI	GATC	5 cut(s)	143, 148, 303, 452, 559
BssT1I	CCWWGG	2 cut(s)	283, 535
Bst2UI	CCWGG	2 cut(s)	23, 82
Bst4CI	ACNGT	5 cut(s)	34, 224, 265, 317, 464
BstAPI	GCANNNNNTGC	1 cut(s)	590
BstC8I	GCNNGC	1 cut(s)	425
BstDEI	CTNAG	4 cut(s)	107, 408, 618, 648
BstDSI	CCRYGG	2 cut(s)	283, 535
BstFNI	CGCG	2 cut(s)	47, 399
BstHHI	GCGC	1 cut(s)	399
BstKTI	GATC	5 cut(s)	146, 151, 306, 455, 562
BstMBI	GATC	5 cut(s)	143, 148, 303, 452, 559
BstMWI	GCNNNNNNNGC	2 cut(s)	361, 590
BstNI	CCWGG	2 cut(s)	23, 82
BstNSI	RCATGY	2 cut(s)	375, 427
BstSCI	CCNGG	3 cut(s)	21, 80, 593
BstSFI	CTRYAG	2 cut(s)	189, 243
BstUI	CGCG	2 cut(s)	47, 399
BstV1I	GCAGC	2 cut(s)	373, 578
BstX2I	RGATCY	2 cut(s)	143, 303
BstYI	RGATCY	2 cut(s)	143, 303
BtgI	CCRYGG	2 cut(s)	283, 535
BtsIMutI	CAGTG	2 cut(s)	115, 229
Cac8I	GCNNGC	1 cut(s)	425
CciI	TCATGA	1 cut(s)	526
CfoI	GCGC	1 cut(s)	399
Cfr13I	GGNCC	1 cut(s)	544
CseI	GACGC	1 cut(s)	129
CsiI	ACCWGGT	1 cut(s)	21
Csp6I	GTAC	4 cut(s)	78, 220, 261, 465
CviAII	CATG	9 cut(s)	58, 176, 284, 332, 372, 424, 527, 536, 563
CviJI	RGCY	7 cut(s)	85, 137, 200, 242, 355, 446, 606
CviKI_1	RGCY	7 cut(s)	85, 137, 200, 242, 355, 446, 606
CviQI	GTAC	4 cut(s)	78, 220, 261, 465
DdeI	CTNAG	4 cut(s)	107, 408, 618, 648
DpnI	GATC	5 cut(s)	145, 150, 305, 454, 561
DpnII	GATC	5 cut(s)	143, 148, 303, 452, 559
Eco130I	CCWWGG	2 cut(s)	283, 535
Eco32I	GATATC	1 cut(s)	6
Eco47I	GGWCC	1 cut(s)	544
EcoRII	CCWGG	2 cut(s)	21, 80
EcoRV	GATATC	1 cut(s)	6
EcoT14I	CCWWGG	2 cut(s)	283, 535
EcoT22I	ATGCAT	1 cut(s)	232
ErhI	CCWWGG	2 cut(s)	283, 535
FaeI	CATG	9 cut(s)	61, 179, 287, 335, 375, 427, 530, 539, 566
FatI	CATG	9 cut(s)	57, 175, 283, 331, 371, 423, 526, 535, 562
FauNDI	CATATG	1 cut(s)	350
Fnu4HI	GCNGC	3 cut(s)	362, 421, 567
Fsp4HI	GCNGC	3 cut(s)	362, 421, 567
FspBI	CTAG	2 cut(s)	117, 655
GlaI	GCGC	1 cut(s)	398
GluI	GCNGC	3 cut(s)	362, 421, 567
HapII	CCGG	2 cut(s)	141, 594
HgaI	GACGC	1 cut(s)	129
HhaI	GCGC	1 cut(s)	399
Hin1II	CATG	9 cut(s)	61, 179, 287, 335, 375, 427, 530, 539, 566
Hin6I	GCGC	1 cut(s)	397
HinP1I	GCGC	1 cut(s)	397
HinfI	GANTC	2 cut(s)	100, 470
HpaII	CCGG	2 cut(s)	141, 594
HphI	GGTGA	6 cut(s)	37, 46, 61, 95, 299, 590
Hpy166II	GTNNAC	2 cut(s)	78, 320
Hpy188I	TCNGA	5 cut(s)	99, 148, 574, 630, 642
Hpy188III	TCNNGA	8 cut(s)	117, 141, 161, 407, 502, 519, 527, 554
Hpy8I	GTNNAC	2 cut(s)	78, 320
HpyAV	CCTTC	2 cut(s)	136, 337
HpyCH4III	ACNGT	5 cut(s)	34, 224, 265, 317, 464
HpyCH4V	TGCA	5 cut(s)	175, 230, 427, 566, 584
HpyF10VI	GCNNNNNNNGC	2 cut(s)	361, 590
HpyF3I	CTNAG	4 cut(s)	107, 408, 618, 648
Hsp92II	CATG	9 cut(s)	61, 179, 287, 335, 375, 427, 530, 539, 566
HspAI	GCGC	1 cut(s)	397
Kpn2I	TCCGGA	1 cut(s)	140
Kzo9I	GATC	5 cut(s)	143, 148, 303, 452, 559
LmnI	GCTCC	2 cut(s)	128, 611
Lsp1109I	GCAGC	2 cut(s)	373, 578
LweI	GCATC	6 cut(s)	217, 241, 524, 571, 578, 580
MabI	ACCWGGT	1 cut(s)	21
MaeI	CTAG	2 cut(s)	117, 655
MaeIII	GTNAC	1 cut(s)	334
MalI	GATC	5 cut(s)	145, 150, 305, 454, 561
MboI	GATC	5 cut(s)	143, 148, 303, 452, 559
MboII	GAAGA	2 cut(s)	260, 569
MflI	RGATCY	2 cut(s)	143, 303
MhlI	GDGCHC	2 cut(s)	56, 97
MlyI	GAGTC	1 cut(s)	479
MmeI	TCCRAC	1 cut(s)	233
MnlI	CCTC	4 cut(s)	116, 317, 568, 619
Mph1103I	ATGCAT	1 cut(s)	232
MroI	TCCGGA	1 cut(s)	140
MseI	TTAA	2 cut(s)	477, 614
MslI	CAYNNNNRTG	2 cut(s)	108, 288
MspA1I	CMGCKG	1 cut(s)	242
MspI	CCGG	2 cut(s)	141, 594
MspR9I	CCNGG	3 cut(s)	23, 82, 595
Mva1269I	GAATGC	1 cut(s)	364
MvaI	CCWGG	2 cut(s)	23, 82
MvnI	CGCG	2 cut(s)	47, 399
MwoI	GCNNNNNNNGC	2 cut(s)	361, 590
NciI	CCSGG	1 cut(s)	595
NcoI	CCATGG	2 cut(s)	283, 535
NdeI	CATATG	1 cut(s)	350
NdeII	GATC	5 cut(s)	143, 148, 303, 452, 559
NlaIII	CATG	9 cut(s)	61, 179, 287, 335, 375, 427, 530, 539, 566
NlaIV	GGNNCC	2 cut(s)	305, 546
NmuCI	GTSAC	1 cut(s)	334
NsiI	ATGCAT	1 cut(s)	232
NspI	RCATGY	2 cut(s)	375, 427
OliI	CACNNNNGTG	1 cut(s)	108
PaeI	GCATGC	1 cut(s)	427
PagI	TCATGA	1 cut(s)	526
PciI	ACATGT	1 cut(s)	371
PctI	GAATGC	1 cut(s)	364
PfeI	GAWTC	1 cut(s)	100
PflMI	CCANNNNNTGG	1 cut(s)	541
PkrI	GCNGC	3 cut(s)	363, 422, 568
PleI	GAGTC	1 cut(s)	478
PpsI	GAGTC	1 cut(s)	478
PscI	ACATGT	1 cut(s)	371
Psp6I	CCWGG	2 cut(s)	21, 80
PspGI	CCWGG	2 cut(s)	21, 80
PspN4I	GGNNCC	2 cut(s)	305, 546
PspPI	GGNCC	1 cut(s)	544
PsuI	RGATCY	2 cut(s)	143, 303
PvuII	CAGCTG	1 cut(s)	242
RsaI	GTAC	4 cut(s)	79, 221, 262, 466
RsaNI	GTAC	4 cut(s)	78, 220, 261, 465
RseI	CAYNNNNRTG	2 cut(s)	108, 288
SaqAI	TTAA	2 cut(s)	477, 614
SatI	GCNGC	3 cut(s)	362, 421, 567
Sau3AI	GATC	5 cut(s)	143, 148, 303, 452, 559
Sau96I	GGNCC	1 cut(s)	544
SchI	GAGTC	1 cut(s)	479
ScrFI	CCNGG	3 cut(s)	23, 82, 595
SduI	GDGCHC	2 cut(s)	56, 97
SetI	ASST	7 cut(s)	24, 108, 139, 190, 244, 341, 608
SexAI	ACCWGGT	1 cut(s)	21
SfaNI	GCATC	6 cut(s)	217, 241, 524, 571, 578, 580
SfcI	CTRYAG	2 cut(s)	189, 243
SinI	GGWCC	1 cut(s)	544
SmiMI	CAYNNNNRTG	2 cut(s)	108, 288
SphI	GCATGC	1 cut(s)	427
SsiI	CCGC	4 cut(s)	47, 275, 301, 421
SspI	AATATT	1 cut(s)	157
SspMI	CTAG	2 cut(s)	117, 655
StyD4I	CCNGG	3 cut(s)	21, 80, 593
StyI	CCWWGG	2 cut(s)	283, 535
TaaI	ACNGT	5 cut(s)	34, 224, 265, 317, 464
TaqI	TCGA	2 cut(s)	451, 511
TatI	WGTACW	2 cut(s)	219, 260
TauI	GCSGC	1 cut(s)	423
TfiI	GAWTC	1 cut(s)	100
Tru1I	TTAA	2 cut(s)	477, 614
Tru9I	TTAA	2 cut(s)	477, 614
TscAI	CASTG	2 cut(s)	115, 229
TseFI	GTSAC	1 cut(s)	334
TseI	GCWGC	2 cut(s)	361, 566
Tsp45I	GTSAC	1 cut(s)	334
TspDTI	ATGAA	2 cut(s)	52, 543
TspRI	CASTG	2 cut(s)	115, 229
Van91I	CCANNNNNTGG	1 cut(s)	541
VpaK11BI	GGWCC	1 cut(s)	544
XbaI	TCTAGA	1 cut(s)	116
XceI	RCATGY	2 cut(s)	375, 427
XspI	CTAG	2 cut(s)	117, 655
Zsp2I	ATGCAT	1 cut(s)	232

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.