pycom02g21200

RING-H2 finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0016383
Physical Location & Seq
Reverse (-)
19378768 .. 19381780
3013 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g21200.1

Sequence Viewer

Length: 582 bp
ATGATATCTTCAGGGATAAACCTGGTGATGACGGTGATTGGGTTCGCGGTGAGCACCATGTTCATCGTGTTCGTGTGCACCAGGCTTGTCTGTGCGAGGATTCACCTCAATGTTTCTAGACGCTCCTTCCCGATAGCTTCCAGATCCGATCTCAGTATTCTGGAGCGGGGATTGCATGGCGTTGAACCTGTAGTAGTAGCCAAGTTTCCAACAAAGAAGTACAGTGATGCATTTTTTTCAGCAGCAGAAGATGCTCAATGCACTGTTTGCCTCGCAGAATACCATGGTGATGATGTATTGCGGATCCTCCCCTATTGTGAGCACTCCTTCCATGTGACCTGTATAGACATATGGCTTCAGCAGCATTCCACATGTCCTGTTTGTAGAATATCATTGCGCGAGTTTCCTGAGAGAAAACGCCGCATGCAACCCTTGTTCAGTTCGGCTATTCGGTCTCATTACGGTACAGAGTCCTTCAATACCCATTCTTATCGCTATCTCTTGAATAGTCGCGCATCAAGAACTCATGAAAACCATGGTATGGACCCCATTCAAGAACGATCATGCAGCATCAGAGGGTGA

Protein Analysis

194

Amino Acids

21.99

Weight (kDa)

8.79

Isoelectric Point (pI)

55.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 85 - 129 4.1e-09 RING-H2 zinc finger domain
zf-ANAPC11 PF12861 85 - 131 5.8e-07 Anaphase-promoting complex subunit 11 RING-H2 finger
zf-RING_2 PF13639 86 - 129 1.9e-13 Ring finger domain
zf-RING_11 PF17123 86 - 115 5.8e-08 RING-like zinc finger
zf-C3HC4_2 PF13923 86 - 128 1e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 87 - 128 6e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016383)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g05950
malus_domestica MD02G1247900.v1.1 MD07G1068500.v1.1
prunus_persica Prupe.2G086200_v2.0.a1
pyrus_communis pycom02g21200 pycom07g05270
rosa_chinensis RchiOBHm_Chr1g0333171
rosa_laevigata RLG00000029621
rosa_roxburghii Rroxscaffold_4G00317720
rosa_rugosa Rorug01G0107900
rosa_samantha Rh1AG130900 Rh1BG100100 Rh1CG124700 Rh1DG136300
rosa_wichuraiana Rw0G011810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 541
AccBSI CCGCTC 1 cut(s) 166
AccII CGCG 3 cut(s) 47, 399, 513
AciI CCGC 4 cut(s) 47, 166, 301, 421
AclWI GGATC 3 cut(s) 138, 298, 311
AcuI CTGAAG 1 cut(s) 341
AfaI GTAC 2 cut(s) 221, 466
AfiI CCNNNNNNNGG 1 cut(s) 541
AflIII ACRYGT 1 cut(s) 371
AgsI TTSAA 4 cut(s) 185, 478, 505, 554
AjnI CCWGG 2 cut(s) 21, 80
AluBI AGCT 1 cut(s) 137
AluI AGCT 1 cut(s) 137
Alw21I GWGCWC 3 cut(s) 56, 80, 324
Alw26I GTCTC 1 cut(s) 459
Alw44I GTGCAC 1 cut(s) 76
AlwI GGATC 3 cut(s) 138, 298, 311
ApaLI GTGCAC 1 cut(s) 76
ApeKI GCWGC 3 cut(s) 242, 361, 567
AspLEI GCGC 2 cut(s) 399, 515
AspS9I GGNCC 1 cut(s) 544
AsuHPI GGTGA 5 cut(s) 37, 46, 61, 95, 299
AvaII GGWCC 1 cut(s) 544
BaeGI GKGCMC 1 cut(s) 80
BamHI GGATCC 1 cut(s) 303
Bbv12I GWGCWC 3 cut(s) 56, 80, 324
BbvI GCAGC 2 cut(s) 254, 373
BciT130I CCWGG 2 cut(s) 23, 82
BcoDI GTCTC 1 cut(s) 459
BfaI CTAG 1 cut(s) 117
BfmI CTRYAG 1 cut(s) 189
BisI GCNGC 4 cut(s) 243, 362, 421, 568
BlsI GCNGC 4 cut(s) 244, 363, 422, 569
Bme1390I CCNGG 2 cut(s) 23, 82
Bme18I GGWCC 1 cut(s) 544
BmgT120I GGNCC 1 cut(s) 544
BmiI GGNNCC 2 cut(s) 305, 546
BmrFI CCNGG 2 cut(s) 23, 82
BmsI GCATC 3 cut(s) 217, 241, 524
BpmI CTGGAG 1 cut(s) 182
BsaI GGTCTC 1 cut(s) 459
BsaJI CCNNGG 2 cut(s) 283, 535
Bsc4I CCNNNNNNNGG 1 cut(s) 541
Bse3DI GCAATG 1 cut(s) 392
BseBI CCWGG 2 cut(s) 23, 82
BseDI CCNNGG 2 cut(s) 283, 535
BseLI CCNNNNNNNGG 1 cut(s) 541
BseMI GCAATG 1 cut(s) 392
BseMII CTCAG 2 cut(s) 166, 399
BseSI GKGCMC 1 cut(s) 80
BseXI GCAGC 2 cut(s) 254, 373
Bsh1236I CGCG 3 cut(s) 47, 399, 513
BsiHKAI GWGCWC 3 cut(s) 56, 80, 324
BslI CCNNNNNNNGG 1 cut(s) 541
BsmAI GTCTC 1 cut(s) 459
BsmI GAATGC 1 cut(s) 364
Bso31I GGTCTC 1 cut(s) 459
Bsp1286I GDGCHC 3 cut(s) 56, 80, 324
Bsp143I GATC 4 cut(s) 143, 148, 303, 560
Bsp19I CCATGG 2 cut(s) 283, 535
BspACI CCGC 4 cut(s) 47, 166, 301, 421
BspCNI CTCAG 2 cut(s) 165, 400
BspFNI CGCG 3 cut(s) 47, 399, 513
BspHI TCATGA 1 cut(s) 526
BspLI GGNNCC 2 cut(s) 305, 546
BspPI GGATC 3 cut(s) 138, 298, 311
BspTNI GGTCTC 1 cut(s) 459
BsrBI CCGCTC 1 cut(s) 166
BsrDI GCAATG 1 cut(s) 392
BssECI CCNNGG 2 cut(s) 283, 535
BssMI GATC 4 cut(s) 143, 148, 303, 560
BssT1I CCWWGG 2 cut(s) 283, 535
Bst2UI CCWGG 2 cut(s) 23, 82
Bst4CI ACNGT 4 cut(s) 34, 224, 265, 464
BstAPI GCANNNNNTGC 2 cut(s) 251, 267
BstC8I GCNNGC 1 cut(s) 425
BstDEI CTNAG 2 cut(s) 152, 408
BstDSI CCRYGG 2 cut(s) 283, 535
BstFNI CGCG 3 cut(s) 47, 399, 513
BstHHI GCGC 2 cut(s) 399, 515
BstKTI GATC 4 cut(s) 146, 151, 306, 563
BstMAI GTCTC 1 cut(s) 459
BstMBI GATC 4 cut(s) 143, 148, 303, 560
BstMWI GCNNNNNNNGC 4 cut(s) 172, 251, 267, 361
BstNI CCWGG 2 cut(s) 23, 82
BstNSI RCATGY 2 cut(s) 375, 427
BstSCI CCNGG 2 cut(s) 21, 80
BstSFI CTRYAG 1 cut(s) 189
BstSLI GKGCMC 1 cut(s) 80
BstUI CGCG 3 cut(s) 47, 399, 513
BstV1I GCAGC 2 cut(s) 254, 373
BstX2I RGATCY 2 cut(s) 143, 303
BstYI RGATCY 2 cut(s) 143, 303
BtgI CCRYGG 2 cut(s) 283, 535
BtsIMutI CAGTG 2 cut(s) 229, 261
Cac8I GCNNGC 1 cut(s) 425
CciI TCATGA 1 cut(s) 526
CfoI GCGC 2 cut(s) 399, 515
Cfr13I GGNCC 1 cut(s) 544
CseI GACGC 1 cut(s) 129
CsiI ACCWGGT 1 cut(s) 21
Csp6I GTAC 2 cut(s) 220, 465
CviAII CATG 9 cut(s) 58, 176, 284, 332, 372, 424, 527, 536, 564
CviJI RGCY 5 cut(s) 85, 137, 200, 355, 446
CviKI_1 RGCY 5 cut(s) 85, 137, 200, 355, 446
CviQI GTAC 2 cut(s) 220, 465
DdeI CTNAG 2 cut(s) 152, 408
DpnI GATC 4 cut(s) 145, 150, 305, 562
DpnII GATC 4 cut(s) 143, 148, 303, 560
Eco130I CCWWGG 2 cut(s) 283, 535
Eco31I GGTCTC 1 cut(s) 459
Eco32I GATATC 1 cut(s) 6
Eco47I GGWCC 1 cut(s) 544
Eco57I CTGAAG 1 cut(s) 341
EcoRII CCWGG 2 cut(s) 21, 80
EcoRV GATATC 1 cut(s) 6
EcoT14I CCWWGG 2 cut(s) 283, 535
EcoT22I ATGCAT 1 cut(s) 232
ErhI CCWWGG 2 cut(s) 283, 535
FaeI CATG 9 cut(s) 61, 179, 287, 335, 375, 427, 530, 539, 567
FatI CATG 9 cut(s) 57, 175, 283, 331, 371, 423, 526, 535, 563
FauI CCCGC 1 cut(s) 159
FauNDI CATATG 1 cut(s) 350
Fnu4HI GCNGC 4 cut(s) 243, 362, 421, 568
Fsp4HI GCNGC 4 cut(s) 243, 362, 421, 568
FspBI CTAG 1 cut(s) 117
GlaI GCGC 2 cut(s) 398, 514
GluI GCNGC 4 cut(s) 243, 362, 421, 568
GsuI CTGGAG 1 cut(s) 182
HgaI GACGC 1 cut(s) 129
HhaI GCGC 2 cut(s) 399, 515
Hin1II CATG 9 cut(s) 61, 179, 287, 335, 375, 427, 530, 539, 567
Hin6I GCGC 2 cut(s) 397, 513
HinP1I GCGC 2 cut(s) 397, 513
HinfI GANTC 2 cut(s) 100, 470
HphI GGTGA 5 cut(s) 37, 46, 61, 95, 299
Hpy166II GTNNAC 1 cut(s) 78
Hpy188I TCNGA 2 cut(s) 148, 575
Hpy188III TCNNGA 9 cut(s) 117, 130, 141, 161, 407, 502, 519, 527, 554
Hpy8I GTNNAC 1 cut(s) 78
HpyAV CCTTC 3 cut(s) 136, 337, 484
HpyCH4III ACNGT 4 cut(s) 34, 224, 265, 464
HpyCH4V TGCA 6 cut(s) 78, 175, 230, 261, 427, 567
HpyF10VI GCNNNNNNNGC 4 cut(s) 172, 251, 267, 361
HpyF3I CTNAG 2 cut(s) 152, 408
Hsp92II CATG 9 cut(s) 61, 179, 287, 335, 375, 427, 530, 539, 567
HspAI GCGC 2 cut(s) 397, 513
Kzo9I GATC 4 cut(s) 143, 148, 303, 560
LmnI GCTCC 2 cut(s) 128, 163
Lsp1109I GCAGC 2 cut(s) 254, 373
LweI GCATC 3 cut(s) 217, 241, 524
MabI ACCWGGT 1 cut(s) 21
MaeI CTAG 1 cut(s) 117
MaeIII GTNAC 1 cut(s) 334
MalI GATC 4 cut(s) 145, 150, 305, 562
MbiI CCGCTC 1 cut(s) 166
MboI GATC 4 cut(s) 143, 148, 303, 560
MboII GAAGA 1 cut(s) 260
MflI RGATCY 2 cut(s) 143, 303
MhlI GDGCHC 3 cut(s) 56, 80, 324
MlyI GAGTC 1 cut(s) 479
MmeI TCCRAC 1 cut(s) 233
MnlI CCTC 5 cut(s) 90, 116, 281, 317, 569
Mph1103I ATGCAT 1 cut(s) 232
MslI CAYNNNNRTG 2 cut(s) 108, 288
MspR9I CCNGG 2 cut(s) 23, 82
Mva1269I GAATGC 1 cut(s) 364
MvaI CCWGG 2 cut(s) 23, 82
MvnI CGCG 3 cut(s) 47, 399, 513
MwoI GCNNNNNNNGC 4 cut(s) 172, 251, 267, 361
NcoI CCATGG 2 cut(s) 283, 535
NdeI CATATG 1 cut(s) 350
NdeII GATC 4 cut(s) 143, 148, 303, 560
NlaIII CATG 9 cut(s) 61, 179, 287, 335, 375, 427, 530, 539, 567
NlaIV GGNNCC 2 cut(s) 305, 546
NmuCI GTSAC 1 cut(s) 334
NsiI ATGCAT 1 cut(s) 232
NspI RCATGY 2 cut(s) 375, 427
PaeI GCATGC 1 cut(s) 427
PagI TCATGA 1 cut(s) 526
PciI ACATGT 1 cut(s) 371
PctI GAATGC 1 cut(s) 364
PfeI GAWTC 1 cut(s) 100
PflMI CCANNNNNTGG 1 cut(s) 541
PkrI GCNGC 4 cut(s) 244, 363, 422, 569
PleI GAGTC 1 cut(s) 478
PpsI GAGTC 1 cut(s) 478
PscI ACATGT 1 cut(s) 371
Psp6I CCWGG 2 cut(s) 21, 80
PspGI CCWGG 2 cut(s) 21, 80
PspN4I GGNNCC 2 cut(s) 305, 546
PspPI GGNCC 1 cut(s) 544
PsuI RGATCY 2 cut(s) 143, 303
RsaI GTAC 2 cut(s) 221, 466
RsaNI GTAC 2 cut(s) 220, 465
RseI CAYNNNNRTG 2 cut(s) 108, 288
SatI GCNGC 4 cut(s) 243, 362, 421, 568
Sau3AI GATC 4 cut(s) 143, 148, 303, 560
Sau96I GGNCC 1 cut(s) 544
SchI GAGTC 1 cut(s) 479
ScrFI CCNGG 2 cut(s) 23, 82
SduI GDGCHC 3 cut(s) 56, 80, 324
SetI ASST 5 cut(s) 24, 108, 139, 190, 341
SexAI ACCWGGT 1 cut(s) 21
SfaNI GCATC 3 cut(s) 217, 241, 524
SfcI CTRYAG 1 cut(s) 189
SinI GGWCC 1 cut(s) 544
SmiMI CAYNNNNRTG 2 cut(s) 108, 288
SphI GCATGC 1 cut(s) 427
SsiI CCGC 4 cut(s) 47, 166, 301, 421
SspMI CTAG 1 cut(s) 117
StyD4I CCNGG 2 cut(s) 21, 80
StyI CCWWGG 2 cut(s) 283, 535
TaaI ACNGT 4 cut(s) 34, 224, 265, 464
TaqII GACCGA 1 cut(s) 441
TatI WGTACW 1 cut(s) 219
TauI GCSGC 1 cut(s) 423
TfiI GAWTC 1 cut(s) 100
TscAI CASTG 2 cut(s) 229, 268
TseFI GTSAC 1 cut(s) 334
TseI GCWGC 3 cut(s) 242, 361, 567
Tsp45I GTSAC 1 cut(s) 334
TspDTI ATGAA 2 cut(s) 52, 543
TspRI CASTG 2 cut(s) 229, 268
Van91I CCANNNNNTGG 1 cut(s) 541
VneI GTGCAC 1 cut(s) 76
VpaK11BI GGWCC 1 cut(s) 544
XbaI TCTAGA 1 cut(s) 116
XceI RCATGY 2 cut(s) 375, 427
XspI CTAG 1 cut(s) 117
Zsp2I ATGCAT 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.