MD07G1132600.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0011486
Physical Location & Seq
Reverse (-)
19180922 .. 19188574
7653 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1132600.v1.1.491

Sequence Viewer

Length: 3015 bp
ATGAGCTTAGAGAATGAAGAACCACACTTCCCAGATCAGCCTACTGAAACGAATCACGAAAAGCAAAAGAAGTCGAAAATATCATATAGGAGAGAATTCCTACTGTCGTTTAGTGAATTAGATTCTTGCAAGAAACTACCAAGTGGGTTCGACCAGTCAATAATAAGTGAATTCGAGGATGCTTTCAAAGATCGACAGAGAACTAGTGGTTTGTCAGCTTATAGTTTCAGGCGAAACGAGTATGGTTCATCTCCACCTACTAGAGGGGATGTAGCTGGTTATTCGCGGCCAATCCATGGAAGGTGGGAAGGTCGTTCAACTGGACGGAGTGACAGGGATAGTGACTCGCAATCTGATAGGGATTCTGATTCCGGAAGGCATTTTGGGAACCAGTCTCGGCGGCCATTGCAGGTTCCTGAGCATGATGGCCTACTCGGAAGTGGCTCCTTTCCTAGACCAGCTGGGTTTACTGGAGGGATATCAGCACCAAAAGTTCGATCTAACGAACCGTACCAGTTGAATAGAACCAATGAGCCATATCACCCTCCCCGTCCTTACAAGGCAGCACCTCACTCACGGAGGGATACTGACTTGTTTAATGATGAAACGTTTGGTTCATCTGAGTTGACAAGTGAGGAAAGAGCAGAAGAGGAAAGAAAGAGAAGAGCTTCATTTGAATTAATGAGGAAAGAACAGCACAAAGCACTTCAAGAGCAGAAGCTGAAGCCAGAGAAGAATAAAGGTGAATTTGACTTTGCTACGCTGGTGGATGATTCCAAAGATGAGAGGAGACATAGAAGCAGTGAAGTAGAGGAGCCTCTCATACCACCAGCTGCAAATACTGATTCTGAAAAATCTAGTTTTCTTTTGCAAACTGCTGCACCCAGACCTCTGGTACCCCCAGGTTTTGCTACCACAGTTTTGGAAAGAAATCTTGGACCAAAATCCTTGAGCGATACCCGTGAAGTTGAGGTTGGGAATTCTGGACTTGAGGAAAATATCTTGCGTGCCAAAACTAAACCTGTTTTAAATGGGACTTCTGATAACCAAGTGGAGAAACAATCAACTGAACAAATGTTCTTGAGCAAGCAACAACATGGAAGTGCAAGTACTGATGTCCCAGTTGATAGCATGGGTGAGAAGAATCAAAATTTGTCACCACCTCAAGGTGCCTTGAATAAGATAATTGGCATTGAGAGTCAACTCTATAACATAGCTAATACATCACAACCTGTAGAAGTCTCCAGAAATAGTGAAGTAATTGACCTTAATGCTGAAAAGGTGATGAGGACTAAGATTGTTGGTGAATCCAATCAAGGTCCTCCTGCGTCAATACTGGAAAAGCTTTTTACCAATGCTGTAGCATTAAATGGAGTTGGCTCCTCTAATACCACTGAGCATCAAGATAGTAAAGATGATGAGACACGGAGCTCTGACACTGCCCATTCTTCCAAGTTTGCTCATTGGTTTCATCAAGAAGAAAGGAAACCCAGTGATGATTTCTCATCCGGAAGGCAAAATGACTTACTTTCATTGATCGTTAGTGGTGAAAAAGGCGGATCCGGCATTACTGCTGGGAAGATACGTGATCACAGGTTTCCTAGTTTTTCTTCTCAAAACTCTGAACCTGCGGACAGGGTTATGAATTCAGATGTATCTCCTACAGTTGGTAGCTCTGAACAATTGTCCAAAAGTAATAAACCAGAGGCAGTTTCGACATGGAGTCCTCCTGGGGCTGGTGGAAAGCCTGAGCAGCTGAAAGCTAATGTTGATAATAATGCATCCCAGCACCTACTTTCACTGTTGCAGAAGGGAACATGCTTGAAAGATATGGAACCATCCTATAATCAAGAAACAATGTATTTTGAAAAATTACACGACACTGAAGGAGCGACTATTGGCACTGCAGTCCGCATTTCAAAAAATGAAATTTCTGAAAATGTTTCTGATGCAGGGAAGAACATGACTCTTGAAACGCTGTTTGGAACTGCTTTTATGAAAGAGTTACAAACAGTTGGAGCACCGGTTTCTGTTAAGAGGGCCCCAATTGGATCTGCAAGAGTAGATCCTATGGAGCCTCACAGTGTTGGAACCGACTTGGGTTCTAAGATTGGGGTCTTTGAGGGGCATCCTGATTTTCGACTTCCTGAGGAGGATGGCTTGATTGCAGCCAGCGAACCGTTGAACATCCAAAATTTTATGTCTTCTGGGAATCAAGTGAAAAGCAAATTGTTCTCCTCCCAGAACACACGGGTTGACATTGTTGAAAAGTTGGCAGCTATGAATTCTGCTTTTAAAGATGAAAGAAGCATGGGAAGTCAAGAAGTTCCTCCTTTTCTTCGTGGTCGTGGTCCTTATGATATAAGAGAACCTGATAATCCATATCAGAACCTTAATGTACAACCACCTTCTCAGCAGCTCCATCATCCTCAATTGAATCATGTGGGTCCCCCGTTCCACCCATTAGACTCTCATCCTGTCAACATCAACTCTCAAATGAGTTTTTTGGGGCCAAAAGGCATTATGCGAAGTGATCCGCCTCCAAATCATCAATTTCATGCAAATATGCTTCGTCCACCTTTTCATCATTCTAACACTGGGCAATCTGGGTTTGATGCTCACACCCATCACCCTATGATGCAACAGATGCGTATGCAAGGCAACTTTCCTCCGCCTCACCTGCTACAAGGATTATCCAGTTCTCCACCCCAGCATCCTCATCCTAATCTGGGTGCAACTCTGCCTGCTCAACCAGTCAGTCAGGCTTTTATGCAAGAATTGAACCCAATGCAAGGTTTCCCCTTTGGTCCCCAGAATCCCAACTTTGGTGGTCATGGAATGCCATCGCCTGCTCCTGACGCTGCCGGTGGAAGCAACCACCATCCAGAGGCGCTCCAAAGGCTTATTGAGATGGAACTACGGTCAAACCCAAAACAGATTCATCAGTTTCCTGCTAGCGGACACACTCAAGGGGCTCACGGACACGAACTTGACATGGGTTTCGGGTATAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1005

Amino Acids

110.79

Weight (kDa)

6.05

Isoelectric Point (pI)

54.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011486)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G01290 AT4G01290
fragaria_vesca FvH4_7g15120
malus_domestica MD01G1069900.v1.1 MD07G1132600.v1.1
prunus_persica Prupe.2G176000_v2.0.a1
pyrus_communis pycom01g09910 pycom07g12940
rosa_chinensis RchiOBHm_Chr1g0357311
rosa_laevigata RLG00000028028
rosa_multiflora Rmu_sc0000316.1_g000002 Rmu_sc0011531.1_g000003
rosa_roxburghii Rroxscaffold_4G00298860
rosa_rugosa Rorug01G0256200
rosa_samantha Rh1AG269600 Rh1BG237500 Rh1CG253800 Rh1DG265400
rosa_wichuraiana Rw1G023880 Rw1G023980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2688
Acc36I ACCTGC 3 cut(s) 400, 1636, 2688
Acc65I GGTACC 1 cut(s) 895
AccB1I GGYRCC 2 cut(s) 895, 1169
AccB7I CCANNNNNTGG 1 cut(s) 296
AccII CGCG 1 cut(s) 286
AccIII TCCGGA 2 cut(s) 371, 1508
AciI CCGC 8 cut(s) 286, 400, 1557, 1631, 1912, 2537, 2672, 2958
AclI AACGTT 1 cut(s) 608
AclWI GGATC 5 cut(s) 1554, 1567, 2059, 2060, 2528
AcoI YGGCCR 2 cut(s) 287, 401
AcsI RAATTY 9 cut(s) 95, 170, 746, 979, 1150, 1645, 1929, 2194, 2284
AcuI CTGAAG 2 cut(s) 743, 1905
AfaI GTAC 4 cut(s) 512, 897, 1111, 2400
AgeI ACCGGT 1 cut(s) 2023
AhdI GACNNNNNGTC 1 cut(s) 1722
AhlI ACTAGT 1 cut(s) 203
AjnI CCWGG 2 cut(s) 901, 1729
AjuI GAANNNNNNNTTGG 6 cut(s) 918, 950, 957, 989, 1965, 1997
AloI GAACNNNNNNTCC 2 cut(s) 12, 44
Alw21I GWGCWC 2 cut(s) 1433, 2023
Alw26I GTCTC 4 cut(s) 399, 784, 1246, 1415
AlwI GGATC 5 cut(s) 1554, 1567, 2059, 2060, 2528
AlwNI CAGNNNCTG 1 cut(s) 721
Aor13HI TCCGGA 2 cut(s) 371, 1508
AoxI GGCC 5 cut(s) 287, 401, 427, 2040, 2510
ApaI GGGCCC 1 cut(s) 2044
ApeKI GCWGC 8 cut(s) 563, 833, 878, 1753, 2168, 2276, 2416, 2861
ApoI RAATTY 9 cut(s) 95, 170, 746, 979, 1150, 1645, 1929, 2194, 2284
ArsI GACNNNNNNTTYG 1 cut(s) 2984
AseI ATTAAT 1 cut(s) 680
AsiGI ACCGGT 1 cut(s) 2023
Asp700I GAANNNNTTC 1 cut(s) 667
Asp718I GGTACC 1 cut(s) 895
AspLEI GCGC 1 cut(s) 2893
AspS9I GGNCC 8 cut(s) 938, 1319, 2040, 2041, 2351, 2447, 2510, 2807
AsuHPI GGTGA 9 cut(s) 533, 755, 1148, 1149, 1294, 1316, 1559, 2621, 2669
AsuNHI GCTAGC 1 cut(s) 2954
AvaII GGWCC 5 cut(s) 938, 1319, 2351, 2447, 2807
AxyI CCTNAGG 1 cut(s) 2148
BaeGI GKGCMC 1 cut(s) 2044
BamHI GGATCC 1 cut(s) 1559
BanI GGYRCC 2 cut(s) 895, 1169
BanII GRGCYC 3 cut(s) 1433, 2044, 2977
BarI GAAGNNNNNNTAC 2 cut(s) 1093, 1125
BbsI GAAGAC 1 cut(s) 2196
Bbv12I GWGCWC 2 cut(s) 1433, 2023
BbvI GCAGC 8 cut(s) 575, 820, 865, 1765, 2180, 2288, 2428, 2848
BccI CCATC 8 cut(s) 419, 1846, 2150, 2430, 2634, 2851, 2889, 2905
BciT130I CCWGG 2 cut(s) 903, 1731
BciVI GTATCC 1 cut(s) 577
BclI TGATCA 1 cut(s) 1588
BcoDI GTCTC 4 cut(s) 399, 784, 1246, 1415
BcuI ACTAGT 1 cut(s) 203
BfaI CTAG 6 cut(s) 204, 261, 453, 858, 1602, 2955
BfmI CTRYAG 4 cut(s) 1233, 1359, 1662, 1905
BfoI RGCGCY 1 cut(s) 2894
BfuAI ACCTGC 3 cut(s) 400, 1636, 2688
BfuI GTATCC 1 cut(s) 577
BmcAI AGTACT 1 cut(s) 1111
Bme1390I CCNGG 2 cut(s) 903, 1731
Bme18I GGWCC 5 cut(s) 938, 1319, 2351, 2447, 2807
BmeRI GACNNNNNGTC 1 cut(s) 1722
BmgT120I GGNCC 8 cut(s) 938, 1319, 2040, 2041, 2351, 2447, 2510, 2807
BmrFI CCNGG 2 cut(s) 903, 1731
BmrI ACTGGG 3 cut(s) 1115, 1485, 2607
BmsI GCATC 9 cut(s) 169, 1408, 1790, 1939, 2137, 2605, 2628, 2637, 2722
BmtI GCTAGC 1 cut(s) 2958
BmuI ACTGGG 3 cut(s) 1115, 1485, 2607
BpiI GAAGAC 1 cut(s) 2196
BpmI CTGGAG 2 cut(s) 492, 1228
Bpu10I CCTNAGC 2 cut(s) 417, 1749
BpuEI CTTGAG 5 cut(s) 970, 1010, 1102, 1149, 2952
BsaAI YACGTR 1 cut(s) 1586
BsaJI CCNNGG 3 cut(s) 295, 901, 1730
BsaWI WCCGGW 3 cut(s) 371, 1508, 2023
BsaXI ACNNNNNCTCC 2 cut(s) 2010, 2040
Bse118I RCCGGY 2 cut(s) 2023, 2864
Bse21I CCTNAGG 1 cut(s) 2148
Bse3DI GCAATG 1 cut(s) 404
BseAI TCCGGA 2 cut(s) 371, 1508
BseBI CCWGG 2 cut(s) 903, 1731
BseDI CCNNGG 3 cut(s) 295, 901, 1730
BseMI GCAATG 1 cut(s) 404
BseMII CTCAG 6 cut(s) 408, 612, 1386, 1740, 2139, 2426
BseRI GAGGAG 5 cut(s) 802, 827, 1372, 2165, 2227
BseSI GKGCMC 1 cut(s) 2044
BseXI GCAGC 8 cut(s) 575, 820, 865, 1765, 2180, 2288, 2428, 2848
BseYI CCCAGC 4 cut(s) 461, 1574, 1785, 2709
BsgI GTGCAG 1 cut(s) 864
Bsh1236I CGCG 1 cut(s) 286
BshFI GGCC 5 cut(s) 289, 403, 429, 2042, 2512
BshNI GGYRCC 2 cut(s) 895, 1169
BshTI ACCGGT 1 cut(s) 2023
BsiHKAI GWGCWC 2 cut(s) 1433, 2023
BsiSI CCGG 5 cut(s) 372, 1509, 1563, 2024, 2865
BslFI GGGAC 4 cut(s) 1048, 1103, 2433, 2793
BsmAI GTCTC 4 cut(s) 399, 784, 1246, 1415
BsmFI GGGAC 4 cut(s) 1048, 1103, 2433, 2793
BsmI GAATGC 1 cut(s) 2844
BsnI GGCC 5 cut(s) 289, 403, 429, 2042, 2512
Bsp120I GGGCCC 1 cut(s) 2040
Bsp1286I GDGCHC 4 cut(s) 1433, 2023, 2044, 2977
Bsp13I TCCGGA 2 cut(s) 371, 1508
Bsp1407I TGTACA 1 cut(s) 2398
Bsp143I GATC 9 cut(s) 34, 190, 497, 1536, 1559, 1588, 2051, 2065, 2533
Bsp19I CCATGG 1 cut(s) 295
BspACI CCGC 8 cut(s) 286, 400, 1557, 1631, 1912, 2537, 2672, 2958
BspANI GGCC 5 cut(s) 289, 403, 429, 2042, 2512
BspCNI CTCAG 6 cut(s) 409, 613, 1387, 1741, 2140, 2425
BspEI TCCGGA 2 cut(s) 371, 1508
BspFNI CGCG 1 cut(s) 286
BspMAI CTGCAG 1 cut(s) 1909
BspMI ACCTGC 3 cut(s) 400, 1636, 2688
BspOI GCTAGC 1 cut(s) 2958
BspPI GGATC 5 cut(s) 1554, 1567, 2059, 2060, 2528
BspQI GCTCTTC 1 cut(s) 658
BspT107I GGYRCC 2 cut(s) 895, 1169
BsrDI GCAATG 1 cut(s) 404
BsrFI RCCGGY 2 cut(s) 2023, 2864
BsrGI TGTACA 1 cut(s) 2398
BssAI RCCGGY 2 cut(s) 2023, 2864
BssECI CCNNGG 3 cut(s) 295, 901, 1730
BssMI GATC 9 cut(s) 34, 190, 497, 1536, 1559, 1588, 2051, 2065, 2533
BssT1I CCWWGG 1 cut(s) 295
Bst2UI CCWGG 2 cut(s) 903, 1731
Bst4CI ACNGT 9 cut(s) 105, 510, 919, 1666, 1803, 2014, 2084, 2181, 2922
Bst6I CTCTTC 2 cut(s) 642, 658
BstAPI GCANNNNNTGC 1 cut(s) 2647
BstAUI TGTACA 1 cut(s) 2398
BstBAI YACGTR 1 cut(s) 1586
BstC8I GCNNGC 6 cut(s) 1008, 1088, 2173, 2745, 2850, 2956
BstDEI CTNAG 9 cut(s) 7, 417, 621, 1293, 1395, 1749, 2106, 2148, 2412
BstDSI CCRYGG 1 cut(s) 295
BstENI CCTNNNNNAGG 1 cut(s) 261
BstFNI CGCG 1 cut(s) 286
BstH2I RGCGCY 1 cut(s) 2894
BstHHI GCGC 1 cut(s) 2893
BstKTI GATC 9 cut(s) 37, 193, 500, 1539, 1562, 1591, 2054, 2068, 2536
BstMAI GTCTC 4 cut(s) 399, 784, 1246, 1415
BstMBI GATC 9 cut(s) 34, 190, 497, 1536, 1559, 1588, 2051, 2065, 2533
BstMWI GCNNNNNNNGC 7 cut(s) 406, 1563, 1753, 2647, 2680, 2858, 2899
BstNI CCWGG 2 cut(s) 903, 1731
BstNSI RCATGY 1 cut(s) 1821
BstSCI CCNGG 2 cut(s) 901, 1729
BstSFI CTRYAG 4 cut(s) 1233, 1359, 1662, 1905
BstSLI GKGCMC 1 cut(s) 2044
BstUI CGCG 1 cut(s) 286
BstV1I GCAGC 8 cut(s) 575, 820, 865, 1765, 2180, 2288, 2428, 2848
BstV2I GAAGAC 1 cut(s) 2196
BstX2I RGATCY 3 cut(s) 1559, 2051, 2065
BstXI CCANNNNNNTGG 2 cut(s) 892, 922
BstYI RGATCY 3 cut(s) 1559, 2051, 2065
Bsu36I CCTNAGG 1 cut(s) 2148
BsuI GTATCC 1 cut(s) 577
BsuRI GGCC 5 cut(s) 289, 403, 429, 2042, 2512
BtgI CCRYGG 1 cut(s) 295
BtgZI GCGATG 1 cut(s) 2829
BtsI GCAGTG 3 cut(s) 808, 1437, 1902
BtsIMutI CAGTG 9 cut(s) 808, 1392, 1437, 1498, 1799, 1881, 1902, 2089, 2595
BveI ACCTGC 3 cut(s) 400, 1636, 2688
Cac8I GCNNGC 6 cut(s) 1008, 1088, 2173, 2745, 2850, 2956
CaiI CAGNNNCTG 1 cut(s) 721
CfoI GCGC 1 cut(s) 2893
Cfr10I RCCGGY 2 cut(s) 2023, 2864
Cfr13I GGNCC 8 cut(s) 938, 1319, 2040, 2041, 2351, 2447, 2510, 2807
CseI GACGC 2 cut(s) 1317, 2867
Csp6I GTAC 4 cut(s) 511, 896, 1110, 2399
CspAI ACCGGT 1 cut(s) 2023
CspCI CAANNNNNGTGG 2 cut(s) 2809, 2844
CviQI GTAC 4 cut(s) 511, 896, 1110, 2399
DdeI CTNAG 9 cut(s) 7, 417, 621, 1293, 1395, 1749, 2106, 2148, 2412
DpnI GATC 9 cut(s) 36, 192, 499, 1538, 1561, 1590, 2053, 2067, 2535
DpnII GATC 9 cut(s) 34, 190, 497, 1536, 1559, 1588, 2051, 2065, 2533
DraI TTTAAA 2 cut(s) 1029, 2296
DriI GACNNNNNGTC 1 cut(s) 1722
EaeI YGGCCR 2 cut(s) 287, 401
Eam1104I CTCTTC 2 cut(s) 642, 658
Eam1105I GACNNNNNGTC 1 cut(s) 1722
EarI CTCTTC 2 cut(s) 642, 658
EciI GGCGGA 3 cut(s) 1572, 2526, 2661
Ecl136II GAGCTC 1 cut(s) 1431
Eco130I CCWWGG 1 cut(s) 295
Eco24I GRGCYC 3 cut(s) 1433, 2044, 2977
Eco32I GATATC 1 cut(s) 480
Eco47I GGWCC 5 cut(s) 938, 1319, 2351, 2447, 2807
Eco53kI GAGCTC 1 cut(s) 1431
Eco57I CTGAAG 2 cut(s) 743, 1905
Eco81I CCTNAGG 1 cut(s) 2148
EcoICRI GAGCTC 1 cut(s) 1431
EcoNI CCTNNNNNAGG 1 cut(s) 261
EcoO109I RGGNCCY 4 cut(s) 1319, 2040, 2041, 2447
EcoRI GAATTC 5 cut(s) 95, 170, 979, 1645, 2284
EcoRII CCWGG 2 cut(s) 901, 1729
EcoRV GATATC 1 cut(s) 480
EcoT14I CCWWGG 1 cut(s) 295
EcoT22I ATGCAT 1 cut(s) 1783
EcoT38I GRGCYC 3 cut(s) 1433, 2044, 2977
ErhI CCWWGG 1 cut(s) 295
FaqI GGGAC 4 cut(s) 1048, 1103, 2433, 2793
FbaI TGATCA 1 cut(s) 1588
FriOI GRGCYC 3 cut(s) 1433, 2044, 2977
FspBI CTAG 6 cut(s) 204, 261, 453, 858, 1602, 2955
GlaI GCGC 1 cut(s) 2892
GsaI CCCAGC 4 cut(s) 465, 1578, 1789, 2713
GsuI CTGGAG 2 cut(s) 492, 1228
HaeII RGCGCY 1 cut(s) 2894
HaeIII GGCC 5 cut(s) 289, 403, 429, 2042, 2512
HapII CCGG 5 cut(s) 372, 1509, 1563, 2024, 2865
HgaI GACGC 2 cut(s) 1317, 2867
HhaI GCGC 1 cut(s) 2893
Hin6I GCGC 1 cut(s) 2891
HinP1I GCGC 1 cut(s) 2891
HincII GTYRAC 4 cut(s) 627, 1202, 2257, 2482
HindII GTYRAC 4 cut(s) 627, 1202, 2257, 2482
HindIII AAGCTT 1 cut(s) 1343
HpaII CCGG 5 cut(s) 372, 1509, 1563, 2024, 2865
HphI GGTGA 9 cut(s) 533, 755, 1148, 1149, 1294, 1316, 1559, 2621, 2669
Hpy166II GTNNAC 6 cut(s) 468, 627, 1202, 2257, 2482, 2576
Hpy8I GTNNAC 6 cut(s) 468, 627, 1202, 2257, 2482, 2576
HpyAV CCTTC 7 cut(s) 294, 302, 369, 1506, 1804, 1880, 2418
HpyCH4III ACNGT 9 cut(s) 105, 510, 919, 1666, 1803, 2014, 2084, 2181, 2922
HpyCH4IV ACGT 2 cut(s) 608, 1585
HpyF10VI GCNNNNNNNGC 7 cut(s) 406, 1563, 1753, 2647, 2680, 2858, 2899
HpyF3I CTNAG 9 cut(s) 7, 417, 621, 1293, 1395, 1749, 2106, 2148, 2412
HpySE526I ACGT 2 cut(s) 608, 1585
HspAI GCGC 1 cut(s) 2891
KflI GGGWCCC 1 cut(s) 2447
Kpn2I TCCGGA 2 cut(s) 371, 1508
KpnI GGTACC 1 cut(s) 899
Ksp22I TGATCA 1 cut(s) 1588
Kzo9I GATC 9 cut(s) 34, 190, 497, 1536, 1559, 1588, 2051, 2065, 2533
LguI GCTCTTC 1 cut(s) 658
Lsp1109I GCAGC 8 cut(s) 575, 820, 865, 1765, 2180, 2288, 2428, 2848
LweI GCATC 9 cut(s) 169, 1408, 1790, 1939, 2137, 2605, 2628, 2637, 2722
MaeI CTAG 6 cut(s) 204, 261, 453, 858, 1602, 2955
MaeII ACGT 2 cut(s) 608, 1585
MaeIII GTNAC 4 cut(s) 329, 341, 1155, 2004
MalI GATC 9 cut(s) 36, 192, 499, 1538, 1561, 1590, 2053, 2067, 2535
MboI GATC 9 cut(s) 34, 190, 497, 1536, 1559, 1588, 2051, 2065, 2533
MfeI CAATTG 3 cut(s) 1682, 2046, 2432
MflI RGATCY 3 cut(s) 1559, 2051, 2065
MhlI GDGCHC 4 cut(s) 1433, 2023, 2044, 2977
MlyI GAGTC 5 cut(s) 338, 1207, 1732, 1960, 2462
MmeI TCCRAC 2 cut(s) 1996, 2068
Mph1103I ATGCAT 1 cut(s) 1783
MroI TCCGGA 2 cut(s) 371, 1508
MroXI GAANNNNTTC 1 cut(s) 667
MseI TTAA 8 cut(s) 597, 680, 1028, 1269, 1367, 2034, 2295, 2394
MslI CAYNNNNRTG 1 cut(s) 1101
MspA1I CMGCKG 3 cut(s) 461, 833, 1756
MspI CCGG 5 cut(s) 372, 1509, 1563, 2024, 2865
MspR9I CCNGG 2 cut(s) 903, 1731
MunI CAATTG 3 cut(s) 1682, 2046, 2432
Mva1269I GAATGC 1 cut(s) 2844
MvaI CCWGG 2 cut(s) 903, 1731
MvnI CGCG 1 cut(s) 286
MwoI GCNNNNNNNGC 7 cut(s) 406, 1563, 1753, 2647, 2680, 2858, 2899
NcoI CCATGG 1 cut(s) 295
NdeII GATC 9 cut(s) 34, 190, 497, 1536, 1559, 1588, 2051, 2065, 2533
NheI GCTAGC 1 cut(s) 2954
NmeAIII GCCGAG 1 cut(s) 376
NmuCI GTSAC 3 cut(s) 329, 341, 1155
NsiI ATGCAT 1 cut(s) 1783
NspI RCATGY 1 cut(s) 1821
PaqCI CACCTGC 1 cut(s) 2688
PciSI GCTCTTC 1 cut(s) 658
PctI GAATGC 1 cut(s) 2844
PdmI GAANNNNTTC 1 cut(s) 667
PflMI CCANNNNNTGG 1 cut(s) 296
PinAI ACCGGT 1 cut(s) 2023
PleI GAGTC 5 cut(s) 338, 1206, 1731, 1960, 2462
PpsI GAGTC 5 cut(s) 338, 1206, 1731, 1960, 2462
Ppu21I YACGTR 1 cut(s) 1586
PpuMI RGGWCCY 2 cut(s) 1319, 2447
PshBI ATTAAT 1 cut(s) 680
Psp124BI GAGCTC 1 cut(s) 1433
Psp1406I AACGTT 1 cut(s) 608
Psp5II RGGWCCY 2 cut(s) 1319, 2447
Psp6I CCWGG 2 cut(s) 901, 1729
PspFI CCCAGC 4 cut(s) 461, 1574, 1785, 2709
PspGI CCWGG 2 cut(s) 901, 1729
PspOMI GGGCCC 1 cut(s) 2040
PspPI GGNCC 8 cut(s) 938, 1319, 2040, 2041, 2351, 2447, 2510, 2807
PspPPI RGGWCCY 2 cut(s) 1319, 2447
PstI CTGCAG 1 cut(s) 1909
PstNI CAGNNNCTG 1 cut(s) 721
PsuI RGATCY 3 cut(s) 1559, 2051, 2065
PvuII CAGCTG 3 cut(s) 461, 833, 1756
RsaI GTAC 4 cut(s) 512, 897, 1111, 2400
RsaNI GTAC 4 cut(s) 511, 896, 1110, 2399
RseI CAYNNNNRTG 1 cut(s) 1101
SacI GAGCTC 1 cut(s) 1433
SapI GCTCTTC 1 cut(s) 658
SaqAI TTAA 8 cut(s) 597, 680, 1028, 1269, 1367, 2034, 2295, 2394
Sau3AI GATC 9 cut(s) 34, 190, 497, 1536, 1559, 1588, 2051, 2065, 2533
Sau96I GGNCC 8 cut(s) 938, 1319, 2040, 2041, 2351, 2447, 2510, 2807
ScaI AGTACT 1 cut(s) 1111
SchI GAGTC 5 cut(s) 338, 1207, 1732, 1960, 2462
ScrFI CCNGG 2 cut(s) 903, 1731
SduI GDGCHC 4 cut(s) 1433, 2023, 2044, 2977
SfaNI GCATC 9 cut(s) 169, 1408, 1790, 1939, 2137, 2605, 2628, 2637, 2722
SfcI CTRYAG 4 cut(s) 1233, 1359, 1662, 1905
SinI GGWCC 5 cut(s) 938, 1319, 2351, 2447, 2807
SmiMI CAYNNNNRTG 1 cut(s) 1101
SmlI CTYRAG 5 cut(s) 949, 989, 1081, 1164, 2967
SmoI CTYRAG 5 cut(s) 949, 989, 1081, 1164, 2967
SpeI ACTAGT 1 cut(s) 203
SsiI CCGC 8 cut(s) 286, 400, 1557, 1631, 1912, 2537, 2672, 2958
SspMI CTAG 6 cut(s) 204, 261, 453, 858, 1602, 2955
SstI GAGCTC 1 cut(s) 1433
StyD4I CCNGG 2 cut(s) 901, 1729
StyI CCWWGG 1 cut(s) 295
TaaI ACNGT 9 cut(s) 105, 510, 919, 1666, 1803, 2014, 2084, 2181, 2922
TaiI ACGT 2 cut(s) 611, 1588
TaqI TCGA 7 cut(s) 74, 150, 174, 193, 496, 1715, 2140
TatI WGTACW 2 cut(s) 1109, 2398
TauI GCSGC 2 cut(s) 289, 403
Tru1I TTAA 8 cut(s) 597, 680, 1028, 1269, 1367, 2034, 2295, 2394
Tru9I TTAA 8 cut(s) 597, 680, 1028, 1269, 1367, 2034, 2295, 2394
TscAI CASTG 9 cut(s) 808, 1399, 1444, 1498, 1806, 1888, 1909, 2089, 2602
TseFI GTSAC 3 cut(s) 329, 341, 1155
TseI GCWGC 8 cut(s) 563, 833, 878, 1753, 2168, 2276, 2416, 2861
Tsp45I GTSAC 3 cut(s) 329, 341, 1155
TspGWI ACGGA 4 cut(s) 340, 592, 1441, 2994
TspRI CASTG 9 cut(s) 808, 1399, 1444, 1498, 1806, 1888, 1909, 2089, 2602
Van91I CCANNNNNTGG 1 cut(s) 296
VpaK11BI GGWCC 5 cut(s) 938, 1319, 2351, 2447, 2807
VspI ATTAAT 1 cut(s) 680
XagI CCTNNNNNAGG 1 cut(s) 261
XapI RAATTY 9 cut(s) 95, 170, 746, 979, 1150, 1645, 1929, 2194, 2284
XceI RCATGY 1 cut(s) 1821
XmnI GAANNNNTTC 1 cut(s) 667
XspI CTAG 6 cut(s) 204, 261, 453, 858, 1602, 2955
ZrmI AGTACT 1 cut(s) 1111
Zsp2I ATGCAT 1 cut(s) 1783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.