Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0012648

Physical Location & Seq

Genomic Coordinates Reverse (-)

20018667 .. 20022616

Gene Structure 3950 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1137500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1956 bp

ATGGCTCAGAATCATGAGAGGGTGCCGGGGAATCTGAGAAAACAGTTTGCTGTTGCTGTGAGGAGTATTAAGTGGAGCTATGCAATTTTCTGGTCATTGTCAACTACACAACAAGGAGTGCTGGAATGGGGTGAGGGGTACTACAATGGAGACATCAAAACCCGAAAAAAAGTTGAAGGTGTGGAACTTAAAACCGATAAAATGGGTTTACAGAGGAATGTGCAACTCAGAGAGCTGTATAAGTCTCTTCTGGAAGGTGAGACTGAGACTGAGCAGCAAGCTAAAGCGCCTTCTGCTGTATTGTCCCCGGAAGATCTCACAGATGCCGAGTGGTATTACTTGCTTTGCATGTCCTTCATATTCAATCCTGGCGAAGGTTTGCCAGGAAGAGCATTGGCAACTGGCCAAACCATTTGGTTGTGCAATGCTCAACATACGGATAGTAAAGTTTTCTCGCGCTCTTTGCTGGCGAAGAGTGCATCTGTTCAGACTGTAGTCTGCTTTCCCTACCTGGGGGGTGTTGTTGAGCTAGGTGTGACTGAGCTGGTATCGGAGGACCTTAATCTCATTCAACACATCAAGGCTTCCTTACTAGATTTTTCAAAGCCTGATTGCTGTGAGAAATCTTCCTCTGCCCCTCACAAACCAGACGATGATTCAGAGCAAATAGTTGCCAAGGTTGACCATGACGTAGTTGATACATTGCCTTTAGAGAACCTATATTCCCCTTCGGAAGAAATCAAATTTGATCAGAGGGGAATTAATGGTTTACTCGGAAACCATGAAGAGGTCAACATGGACTCTTCTGATGAATGTTCTAATGGTTGTGATCACAATCATCCGACAGAAGACTCCATGATGCTTGAAGGTACCAATGCTGTGGCTTCTCAGGTTCAGAGTTGGCATTTCATGGATGAAGACTTCAGCAGTGGCGTTCAAGATTCCATGAATTCTAGTGACTCCATATCTGAAGCTTTTGTTAATCAAGGAAAGGCTCACTCTTTTGCTAAACACGAGAATGCGAACCATATCCATTTAAAGGAACTTCAAAACTTCAATGACACAAAACTAAGTTCTTTGTATCTTGGATCTGTTGATGAACATGTACACTACAAAAGAACTCTTTCTACTCTTCTAGGAAGCTCAATGAAGCTGATTGAAAATCCATGTTTTTGCAACGGAGAGAGCAAATCCAGTTTTGTGAAATGGAAGAAAGAAGTTGTTGGTAGTTGTAGGCCAACAGTACATCAGAAGACATTAAAGAAGATTTTGTTCACTGTTCCTTTAATGTATGGTGTTCACTCGCCTATGGCAACCGGTAAAGAAAATACGGGAAAAGATTTGCTCCCAAATTTGCAAGGTGACGATATTAACAGGGAACATGATAAAATGAGAGAGAACGCAAAATTGTTGGTCCTCAGGTCAATGGTTCCTTCTATCACTGAGGTTGATAAAGCTTCGATCCTTGATGATACGATCAAGTACTTGAAAGAGCTTGAGGCACGAGCAGAAGAGATGGAATCCTGCATGGACACCGTGGAAGCTATTTCTAGAGGGAAATTCCTGAACAGGGTAGAGAAGACATCAGATAACTATGATAAAACAAAGAAGAACAATGTGAAAAAGTCTTTAGTAAAGAAGAGAAAGGCCTGTGACATTGACGAAACTGACCCATATCCCAATATGCTTGTTTCCGGAGAAAGCTTGCCACTAGATGTGAAAGTGTGTGTAAAAGAGCAAGAGGTTCTGATAGAGATGAGATGCCCTTACCGGGAATATATCTTGCTTGATATAATGGATGCCATTAACAATCTGTACTTAGATGCACATTCGGTCCAATCATCCATCCTTGACGGTGTTCTCACATTGAGCCTTAAATCAAAGTTTCGAGGAGCGGCGATTTCACCAGTGGGGATGATTAAGCAGGTGCTTTGGAAAATTGCTGGTAAGTGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HLH bHLH-MYC_N

Protein Analysis

652

Amino Acids

72.89

Weight (kDa)

5.54

Isoelectric Point (pI)

39.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
bHLH-MYC_N	PF14215	12 - 195	1.1e-52	bHLH-MYC and R2R3-MYB transcription factors N-terminal
bHLH-TF_ACT-like_plant	PF22754	572 - 647	1.3e-17	Plant bHLH transcription factor, ACT-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012648)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g14230 FvH4_7g14230 FvH4_7g14230
malus_domestica	MD01G1060200.v1.1 MD07G1137500.v1.1
prunus_persica	Prupe.2G170100_v2.0.a1 Prupe.2G170100_v2.0.a1
pyrus_communis	pycom01g09030 pycom07g13540
rosa_chinensis	RchiOBHm_Chr1g0355211
rosa_laevigata	RLG00000028171
rosa_multiflora	Rmu_sc0003115.1_g000041
rosa_roxburghii	Rroxscaffold_4G00300600
rosa_rugosa	Rorug01G0245600
rosa_samantha	Rh1AG258000 Rh1BG227600 Rh1CG241700 Rh1DG255400
rosa_wichuraiana	Rw1G022760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1915
Acc36I	ACCTGC	1 cut(s)	1915
Acc65I	GGTACC	1 cut(s)	869
AccB1I	GGYRCC	2 cut(s)	22, 869
AccBSI	CCGCTC	1 cut(s)	1895
AccII	CGCG	1 cut(s)	457
AccIII	TCCGGA	1 cut(s)	1694
AciI	CCGC	1 cut(s)	1895
AclWI	GGATC	2 cut(s)	1096, 1456
AcoI	YGGCCR	1 cut(s)	403
AcsI	RAATTY	4 cut(s)	743, 949, 1351, 1559
AcuI	CTGAAG	2 cut(s)	907, 990
AfaI	GTAC	6 cut(s)	140, 871, 1107, 1245, 1484, 1817
AfiI	CCNNNNNNNGG	7 cut(s)	374, 512, 513, 787, 1039, 1570, 1771
AflIII	ACRYGT	1 cut(s)	1102
AgeI	ACCGGT	1 cut(s)	1316
AjnI	CCWGG	3 cut(s)	367, 382, 510
AloI	GAACNNNNNNTCC	2 cut(s)	707, 739
Alw26I	GTCTC	4 cut(s)	144, 249, 254, 260
AlwI	GGATC	2 cut(s)	1096, 1456
Aor13HI	TCCGGA	1 cut(s)	1694
AoxI	GGCC	3 cut(s)	403, 1235, 1647
ApeKI	GCWGC	1 cut(s)	274
ApoI	RAATTY	4 cut(s)	743, 949, 1351, 1559
AseI	ATTAAT	1 cut(s)	762
AsiGI	ACCGGT	1 cut(s)	1316
Asp700I	GAANNNNTTC	1 cut(s)	1123
Asp718I	GGTACC	1 cut(s)	869
AspLEI	GCGC	2 cut(s)	289, 459
AspS9I	GGNCC	3 cut(s)	556, 1414, 1834
AsuC2I	CCSGG	3 cut(s)	27, 308, 1772
AsuHPI	GGTGA	4 cut(s)	143, 269, 1373, 1896
AvaII	GGWCC	3 cut(s)	556, 1414, 1834
AxyI	CCTNAGG	1 cut(s)	1418
BalI	TGGCCA	1 cut(s)	405
BanI	GGYRCC	2 cut(s)	22, 869
BauI	CACGAG	2 cut(s)	1013, 1503
BbsI	GAAGAC	4 cut(s)	855, 924, 1259, 1586
BbvI	GCAGC	1 cut(s)	286
BccI	CCATC	2 cut(s)	1510, 1853
BciT130I	CCWGG	3 cut(s)	369, 384, 512
BclI	TGATCA	2 cut(s)	748, 829
BcnI	CCSGG	3 cut(s)	27, 308, 1772
BcoDI	GTCTC	4 cut(s)	144, 249, 254, 260
BfaI	CTAG	6 cut(s)	530, 593, 954, 1136, 1551, 1712
BfmI	CTRYAG	1 cut(s)	492
BfoI	RGCGCY	1 cut(s)	290
BfuAI	ACCTGC	1 cut(s)	1915
BglII	AGATCT	1 cut(s)	313
BisI	GCNGC	2 cut(s)	275, 1896
BlsI	GCNGC	2 cut(s)	276, 1897
BmcAI	AGTACT	1 cut(s)	1484
Bme1390I	CCNGG	6 cut(s)	27, 308, 369, 384, 512, 1772
Bme18I	GGWCC	3 cut(s)	556, 1414, 1834
BmgT120I	GGNCC	3 cut(s)	556, 1414, 1834
BmiI	GGNNCC	3 cut(s)	24, 871, 1431
BmrFI	CCNGG	6 cut(s)	27, 308, 369, 384, 512, 1772
BmsI	GCATC	6 cut(s)	313, 488, 849, 1751, 1789, 1813
BoxI	GACNNNNGTC	1 cut(s)	494
BpiI	GAAGAC	4 cut(s)	855, 924, 1259, 1586
BpuEI	CTTGAG	1 cut(s)	1517
BpuMI	CCSGG	3 cut(s)	27, 308, 1772
BsaBI	GATNNNNATC	1 cut(s)	834
BsaJI	CCNNGG	5 cut(s)	26, 306, 511, 675, 1536
BsaWI	WCCGGW	2 cut(s)	1316, 1694
Bsc4I	CCNNNNNNNGG	7 cut(s)	374, 512, 513, 787, 1039, 1570, 1771
Bse118I	RCCGGY	1 cut(s)	1316
Bse1I	ACTGG	3 cut(s)	406, 1194, 1907
Bse21I	CCTNAGG	1 cut(s)	1418
Bse3DI	GCAATG	2 cut(s)	430, 701
Bse8I	GATNNNNATC	1 cut(s)	834
BseAI	TCCGGA	1 cut(s)	1694
BseBI	CCWGG	3 cut(s)	369, 384, 512
BseDI	CCNNGG	5 cut(s)	26, 306, 511, 675, 1536
BseGI	GGATG	6 cut(s)	838, 919, 1804, 1841, 1845, 1920
BseJI	GATNNNNATC	1 cut(s)	834
BseLI	CCNNNNNNNGG	7 cut(s)	374, 512, 513, 787, 1039, 1570, 1771
BseMI	GCAATG	2 cut(s)	430, 701
BseMII	CTCAG	9 cut(s)	20, 26, 241, 255, 261, 531, 902, 1432, 1434
BseNI	ACTGG	3 cut(s)	406, 1194, 1907
BseRI	GAGGAG	2 cut(s)	76, 1905
BseXI	GCAGC	1 cut(s)	286
Bsh1236I	CGCG	1 cut(s)	457
BshFI	GGCC	3 cut(s)	405, 1237, 1649
BshNI	GGYRCC	2 cut(s)	22, 869
BshTI	ACCGGT	1 cut(s)	1316
BsiSI	CCGG	5 cut(s)	26, 308, 1317, 1695, 1771
BslFI	GGGAC	1 cut(s)	289
BslI	CCNNNNNNNGG	7 cut(s)	374, 512, 513, 787, 1039, 1570, 1771
BsmAI	GTCTC	4 cut(s)	144, 249, 254, 260
BsmFI	GGGAC	1 cut(s)	289
BsmI	GAATGC	1 cut(s)	1024
BsnI	GGCC	3 cut(s)	405, 1237, 1649
Bsp13I	TCCGGA	1 cut(s)	1694
Bsp1407I	TGTACA	1 cut(s)	1105
Bsp143I	GATC	6 cut(s)	313, 748, 829, 1088, 1461, 1476
BspACI	CCGC	1 cut(s)	1895
BspANI	GGCC	3 cut(s)	405, 1237, 1649
BspCNI	CTCAG	9 cut(s)	19, 27, 240, 256, 262, 532, 901, 1431, 1435
BspEI	TCCGGA	1 cut(s)	1694
BspFNI	CGCG	1 cut(s)	457
BspHI	TCATGA	1 cut(s)	13
BspLI	GGNNCC	3 cut(s)	24, 871, 1431
BspMI	ACCTGC	1 cut(s)	1915
BspPI	GGATC	2 cut(s)	1096, 1456
BspQI	GCTCTTC	1 cut(s)	382
BspT107I	GGYRCC	2 cut(s)	22, 869
BsrBI	CCGCTC	1 cut(s)	1895
BsrDI	GCAATG	2 cut(s)	430, 701
BsrFI	RCCGGY	1 cut(s)	1316
BsrGI	TGTACA	1 cut(s)	1105
BsrI	ACTGG	3 cut(s)	406, 1194, 1907
BssAI	RCCGGY	1 cut(s)	1316
BssECI	CCNNGG	5 cut(s)	26, 306, 511, 675, 1536
BssMI	GATC	6 cut(s)	313, 748, 829, 1088, 1461, 1476
BssSI	CACGAG	2 cut(s)	1013, 1503
BssT1I	CCWWGG	1 cut(s)	675
Bst2BI	CACGAG	2 cut(s)	1013, 1503
Bst2UI	CCWGG	3 cut(s)	369, 384, 512
Bst4CI	ACNGT	6 cut(s)	45, 493, 1243, 1279, 1537, 1856
Bst6I	CTCTTC	8 cut(s)	252, 382, 467, 780, 808, 1137, 1506, 1634
BstAUI	TGTACA	1 cut(s)	1105
BstC8I	GCNNGC	3 cut(s)	279, 468, 1706
BstDSI	CCRYGG	1 cut(s)	1536
BstENI	CCTNNNNNAGG	1 cut(s)	372
BstF5I	GGATG	6 cut(s)	838, 919, 1804, 1841, 1845, 1920
BstFNI	CGCG	1 cut(s)	457
BstH2I	RGCGCY	1 cut(s)	290
BstHHI	GCGC	2 cut(s)	289, 459
BstKTI	GATC	6 cut(s)	316, 751, 832, 1091, 1464, 1479
BstMAI	GTCTC	4 cut(s)	144, 249, 254, 260
BstMBI	GATC	6 cut(s)	313, 748, 829, 1088, 1461, 1476
BstMWI	GCNNNNNNNGC	3 cut(s)	293, 463, 476
BstNI	CCWGG	3 cut(s)	369, 384, 512
BstNSI	RCATGY	2 cut(s)	352, 1106
BstPAI	GACNNNNGTC	1 cut(s)	494
BstSCI	CCNGG	6 cut(s)	25, 306, 367, 382, 510, 1770
BstSFI	CTRYAG	1 cut(s)	492
BstUI	CGCG	1 cut(s)	457
BstV1I	GCAGC	1 cut(s)	286
BstV2I	GAAGAC	4 cut(s)	855, 924, 1259, 1586
BstX2I	RGATCY	2 cut(s)	313, 1088
BstXI	CCANNNNNNTGG	1 cut(s)	880
BstYI	RGATCY	2 cut(s)	313, 1088
Bsu36I	CCTNAGG	1 cut(s)	1418
BsuRI	GGCC	3 cut(s)	405, 1237, 1649
BtgI	CCRYGG	1 cut(s)	1536
BtsCI	GGATG	6 cut(s)	838, 919, 1804, 1841, 1845, 1920
BtsI	GCAGTG	1 cut(s)	934
BtsIMutI	CAGTG	4 cut(s)	934, 1275, 1440, 1914
BveI	ACCTGC	1 cut(s)	1915
Cac8I	GCNNGC	3 cut(s)	279, 468, 1706
CciI	TCATGA	1 cut(s)	13
CfoI	GCGC	2 cut(s)	289, 459
Cfr10I	RCCGGY	1 cut(s)	1316
Cfr13I	GGNCC	3 cut(s)	556, 1414, 1834
Csp6I	GTAC	6 cut(s)	139, 870, 1106, 1244, 1483, 1816
CspAI	ACCGGT	1 cut(s)	1316
CviQI	GTAC	6 cut(s)	139, 870, 1106, 1244, 1483, 1816
DpnI	GATC	6 cut(s)	315, 750, 831, 1090, 1463, 1478
DpnII	GATC	6 cut(s)	313, 748, 829, 1088, 1461, 1476
DraI	TTTAAA	1 cut(s)	1038
EaeI	YGGCCR	1 cut(s)	403
Eam1104I	CTCTTC	8 cut(s)	252, 382, 467, 780, 808, 1137, 1506, 1634
EarI	CTCTTC	8 cut(s)	252, 382, 467, 780, 808, 1137, 1506, 1634
Eco130I	CCWWGG	1 cut(s)	675
Eco147I	AGGCCT	1 cut(s)	1649
Eco47I	GGWCC	3 cut(s)	556, 1414, 1834
Eco57I	CTGAAG	2 cut(s)	907, 990
Eco81I	CCTNAGG	1 cut(s)	1418
EcoNI	CCTNNNNNAGG	1 cut(s)	372
EcoO109I	RGGNCCY	1 cut(s)	556
EcoRI	GAATTC	1 cut(s)	949
EcoRII	CCWGG	3 cut(s)	367, 382, 510
EcoT14I	CCWWGG	1 cut(s)	675
ErhI	CCWWGG	1 cut(s)	675
FalI	AAGNNNNNCTT	2 cut(s)	572, 604
FaqI	GGGAC	1 cut(s)	289
FbaI	TGATCA	2 cut(s)	748, 829
Fnu4HI	GCNGC	2 cut(s)	275, 1896
FokI	GGATG	6 cut(s)	825, 926, 1811, 1828, 1832, 1927
Fsp4HI	GCNGC	2 cut(s)	275, 1896
FspBI	CTAG	6 cut(s)	530, 593, 954, 1136, 1551, 1712
GlaI	GCGC	2 cut(s)	288, 458
GluI	GCNGC	2 cut(s)	275, 1896
HaeII	RGCGCY	1 cut(s)	290
HaeIII	GGCC	3 cut(s)	405, 1237, 1649
HapII	CCGG	5 cut(s)	26, 308, 1317, 1695, 1771
HhaI	GCGC	2 cut(s)	289, 459
Hin6I	GCGC	2 cut(s)	287, 457
HinP1I	GCGC	2 cut(s)	287, 457
HincII	GTYRAC	3 cut(s)	102, 682, 793
HindII	GTYRAC	3 cut(s)	102, 682, 793
HindIII	AAGCTT	3 cut(s)	972, 1455, 1702
HinfI	GANTC	8 cut(s)	10, 31, 656, 800, 851, 941, 959, 1520
HpaII	CCGG	5 cut(s)	26, 308, 1317, 1695, 1771
HphI	GGTGA	4 cut(s)	143, 269, 1373, 1896
Hpy166II	GTNNAC	8 cut(s)	102, 209, 682, 770, 793, 1108, 1275, 1300
Hpy188III	TCNNGA	6 cut(s)	14, 251, 938, 1551, 1564, 1695
Hpy8I	GTNNAC	8 cut(s)	102, 209, 682, 770, 793, 1108, 1275, 1300
HpyAV	CCTTC	8 cut(s)	170, 248, 300, 364, 368, 738, 860, 1443
HpyCH4III	ACNGT	6 cut(s)	45, 493, 1243, 1279, 1537, 1856
HpyCH4IV	ACGT	1 cut(s)	690
HpyCH4V	TGCA	9 cut(s)	83, 223, 348, 423, 479, 1176, 1357, 1527, 1826
HpyF10VI	GCNNNNNNNGC	3 cut(s)	293, 463, 476
HpySE526I	ACGT	1 cut(s)	690
HspAI	GCGC	2 cut(s)	287, 457
Kpn2I	TCCGGA	1 cut(s)	1694
KpnI	GGTACC	1 cut(s)	873
Ksp22I	TGATCA	2 cut(s)	748, 829
Kzo9I	GATC	6 cut(s)	313, 748, 829, 1088, 1461, 1476
LguI	GCTCTTC	1 cut(s)	382
LmnI	GCTCC	3 cut(s)	75, 1350, 1892
Lsp1109I	GCAGC	1 cut(s)	286
LweI	GCATC	6 cut(s)	313, 488, 849, 1751, 1789, 1813
MaeI	CTAG	6 cut(s)	530, 593, 954, 1136, 1551, 1712
MaeII	ACGT	1 cut(s)	690
MaeIII	GTNAC	4 cut(s)	535, 956, 1361, 1652
MalI	GATC	6 cut(s)	315, 750, 831, 1090, 1463, 1478
MbiI	CCGCTC	1 cut(s)	1895
MboI	GATC	6 cut(s)	313, 748, 829, 1088, 1461, 1476
MflI	RGATCY	2 cut(s)	313, 1088
MlsI	TGGCCA	1 cut(s)	405
MluCI	AATT	8 cut(s)	84, 743, 759, 949, 1351, 1406, 1559, 1938
MluNI	TGGCCA	1 cut(s)	405
MlyI	GAGTC	3 cut(s)	794, 845, 953
MmeI	TCCRAC	1 cut(s)	866
Mox20I	TGGCCA	1 cut(s)	405
MroI	TCCGGA	1 cut(s)	1694
MroXI	GAANNNNTTC	1 cut(s)	1123
MscI	TGGCCA	1 cut(s)	405
MslI	CAYNNNNRTG	1 cut(s)	1017
Msp20I	TGGCCA	1 cut(s)	405
MspI	CCGG	5 cut(s)	26, 308, 1317, 1695, 1771
MspR9I	CCNGG	6 cut(s)	27, 308, 369, 384, 512, 1772
Mva1269I	GAATGC	1 cut(s)	1024
MvaI	CCWGG	3 cut(s)	369, 384, 512
MvnI	CGCG	1 cut(s)	457
MwoI	GCNNNNNNNGC	3 cut(s)	293, 463, 476
NciI	CCSGG	3 cut(s)	27, 308, 1772
NdeII	GATC	6 cut(s)	313, 748, 829, 1088, 1461, 1476
NlaIV	GGNNCC	3 cut(s)	24, 871, 1431
NmeAIII	GCCGAG	1 cut(s)	352
NmuCI	GTSAC	4 cut(s)	535, 956, 1361, 1652
NspI	RCATGY	2 cut(s)	352, 1106
PagI	TCATGA	1 cut(s)	13
PaqCI	CACCTGC	1 cut(s)	1915
PceI	AGGCCT	1 cut(s)	1649
PciI	ACATGT	1 cut(s)	1102
PciSI	GCTCTTC	1 cut(s)	382
PctI	GAATGC	1 cut(s)	1024
PdmI	GAANNNNTTC	1 cut(s)	1123
PfeI	GAWTC	5 cut(s)	10, 31, 656, 941, 1520
PinAI	ACCGGT	1 cut(s)	1316
PkrI	GCNGC	2 cut(s)	276, 1897
PleI	GAGTC	3 cut(s)	794, 845, 953
PpsI	GAGTC	3 cut(s)	794, 845, 953
PpuMI	RGGWCCY	1 cut(s)	556
PscI	ACATGT	1 cut(s)	1102
PshAI	GACNNNNGTC	1 cut(s)	494
PshBI	ATTAAT	1 cut(s)	762
Psp5II	RGGWCCY	1 cut(s)	556
Psp6I	CCWGG	3 cut(s)	367, 382, 510
PspGI	CCWGG	3 cut(s)	367, 382, 510
PspN4I	GGNNCC	3 cut(s)	24, 871, 1431
PspPI	GGNCC	3 cut(s)	556, 1414, 1834
PspPPI	RGGWCCY	1 cut(s)	556
PsrI	GAACNNNNNNTAC	2 cut(s)	1111, 1143
PsuI	RGATCY	2 cut(s)	313, 1088
RsaI	GTAC	6 cut(s)	140, 871, 1107, 1245, 1484, 1817
RsaNI	GTAC	6 cut(s)	139, 870, 1106, 1244, 1483, 1816
RseI	CAYNNNNRTG	1 cut(s)	1017
SapI	GCTCTTC	1 cut(s)	382
SatI	GCNGC	2 cut(s)	275, 1896
Sau3AI	GATC	6 cut(s)	313, 748, 829, 1088, 1461, 1476
Sau96I	GGNCC	3 cut(s)	556, 1414, 1834
ScaI	AGTACT	1 cut(s)	1484
SchI	GAGTC	3 cut(s)	794, 845, 953
ScrFI	CCNGG	6 cut(s)	27, 308, 369, 384, 512, 1772
SfaNI	GCATC	6 cut(s)	313, 488, 849, 1751, 1789, 1813
SfcI	CTRYAG	1 cut(s)	492
SinI	GGWCC	3 cut(s)	556, 1414, 1834
SmiMI	CAYNNNNRTG	1 cut(s)	1017
SmlI	CTYRAG	1 cut(s)	1496
SmoI	CTYRAG	1 cut(s)	1496
Sse9I	AATT	8 cut(s)	84, 743, 759, 949, 1351, 1406, 1559, 1938
SseBI	AGGCCT	1 cut(s)	1649
SsiI	CCGC	1 cut(s)	1895
SspMI	CTAG	6 cut(s)	530, 593, 954, 1136, 1551, 1712
StuI	AGGCCT	1 cut(s)	1649
StyD4I	CCNGG	6 cut(s)	25, 306, 367, 382, 510, 1770
StyI	CCWWGG	1 cut(s)	675
TaaI	ACNGT	6 cut(s)	45, 493, 1243, 1279, 1537, 1856
TaiI	ACGT	1 cut(s)	693
TaqI	TCGA	2 cut(s)	1460, 1888
TaqII	GACCGA	1 cut(s)	1822
TasI	AATT	8 cut(s)	84, 743, 759, 949, 1351, 1406, 1559, 1938
TatI	WGTACW	4 cut(s)	1105, 1243, 1482, 1815
TauI	GCSGC	1 cut(s)	1898
TfiI	GAWTC	5 cut(s)	10, 31, 656, 941, 1520
TscAI	CASTG	4 cut(s)	934, 1282, 1447, 1914
TseFI	GTSAC	4 cut(s)	535, 956, 1361, 1652
TseI	GCWGC	1 cut(s)	274
Tsp45I	GTSAC	4 cut(s)	535, 956, 1361, 1652
TspDTI	ATGAA	8 cut(s)	346, 798, 825, 898, 930, 962, 1113, 1163
TspGWI	ACGGA	2 cut(s)	452, 1194
TspRI	CASTG	4 cut(s)	934, 1282, 1447, 1914
VpaK11BI	GGWCC	3 cut(s)	556, 1414, 1834
VspI	ATTAAT	1 cut(s)	762
XagI	CCTNNNNNAGG	1 cut(s)	372
XapI	RAATTY	4 cut(s)	743, 949, 1351, 1559
XbaI	TCTAGA	1 cut(s)	1550
XceI	RCATGY	2 cut(s)	352, 1106
XmnI	GAANNNNTTC	1 cut(s)	1123
XspI	CTAG	6 cut(s)	530, 593, 954, 1136, 1551, 1712
ZrmI	AGTACT	1 cut(s)	1484

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.