Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0015794

Physical Location & Seq

Genomic Coordinates Reverse (-)

21887777 .. 21889240

Gene Structure 1464 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1150600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1464 bp

ATGGAAGGGAAAGCAATACTTCCCCTTCTCTTCCTTTCCCTCTCCGCCATTTTCCTCTCCCTCTCCTCCGCCCTCGACCACCAGACCCTCCTCCTCAACCCCCTCCCCTCCCCACCCACCACCCTCTCATGGTCCGACACCGTCTCCGAATCCGATTCCGCCCAACTCCACCCCAACTCCCCCTCCGCCACCGCCACAACCCCAAACACTCTCTCCGTACAATTACACCACATCGACGCCATGGCGCTTAACAAAACCCCCACCCAACTCTTCCACCTCCGCCTCCAGCGCGACGCCTTCCGTGTCAAAGCCCTTACCACCCTCGCCGCCGCCGCGGCGTCCCCAAACAGAACAACCAGAGGCGGTGGTCACGTGCCTGTCACGGGCTTCAGCAGCTCCGTCGTCTCCGGACTCTCTCAGGGCAGCGGCGAGTACTTCACGCGCCTTGGCGTCGGCACGCCTCCCAGGTACGTCTACATGGTCCTCGACACCGGAAGCGACGTCGTTTGGCTCCAATGCGCCCCCTGCAAGCGGTGCTACACCCAGACCGACCCCATTTTCGACCCACGTAAATCCGGATCCTTCGCCACAATCCCCTGTGGGTCCCCCCTCTGCCGGAAACTCGACTCGCCGGGCTGCAATTCCAAGAAGACCTGCCTCTACCAAGTCTCCTACGGGGACGGTTCCTTCACTGTCGGCGAGTTCTCCACCGAAACGCTGACGTTTCGCGGCACCAAAGTGGGACGCGTGGCGATCGGATGCGGTCACGACAACGAGGGCCTCTTCGTCGGTGCGGCGGGGCTTTTGGGACTCGGCCGGGGGAAGTTATCATTTCCCGCCCAGACCGGCTCCCAATTCAACCGGAAATTCTCCTACTGCCTAGTGGACCGGTCCTCTTCTTCCAAACCGTCCTCAGTCCTCTTCGGTGACTCGGCCGTTTCCCGGACCGCCCGGTTCACCCCCCTCCTCGCGAACCCAAAACTCGACACGTTTTACTACGTCGAGCTCATCGGTATCAGTGTCGGTGGGACACGTGTCCGGGGTATCACGGCATCGCTTTTCAATCTCGACCCGGCAGGAAACGGAGGGGTCATCCTCGATTCGGGCACTTCCGTGACCCGATTGACCCGATCGGCTTACAATGCTCTCCGCGATGCTTTCCGGGCCGGTACTTCGGGGCTTAAGCGTGGGCCCGAGTTCTCTCTGTTCGACACGTGTTTCGACTTGTCCGGAAAGACCGAAGTCAAAGTCCCCACGGTCGTGCTGCATTTCCGAAACGCGGACGTTTCACTTCCGGCAACCAACTACCTGATCCCAGTGGACAGTAGCGGCAGCTTCTGCTTTGCTTTCGCGGGTACGATGGGCGGGTTGTCCATAATTGGTAACATCCAGCAGCAAGGTTTCCGGGTGGTGTATGACCTGGCGGGCTCCCGGGTCGGGTTTTCTCCACGTGGGTGCGCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004190 GO:0005575 GO:0005618 GO:0005623 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0009056 GO:0009057 GO:0009505 GO:0009628 GO:0016020 GO:0016787 GO:0019538 GO:0030163 GO:0030312 GO:0043170 GO:0044238 GO:0044464 GO:0050896 GO:0070001 GO:0070011 GO:0071704 GO:0071944 GO:0080167 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko04071 ko04140 ko04142 ko04210 ko04915 ko05152 map04071 map04140 map04142 map04210 map04915 map05152

Pfam Domains

Protein Families

TAXi_C TAXi_N

Protein Analysis

488

Amino Acids

51.63

Weight (kDa)

9.18

Isoelectric Point (pI)

33.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Asp	PF00026	144 - 196	2e-06	Eukaryotic aspartyl protease
TAXi_N	PF14543	145 - 310	4.9e-56	Xylanase inhibitor N-terminal
Asp	PF00026	221 - 483	1.9e-13	Eukaryotic aspartyl protease
TAXi_C	PF14541	332 - 482	1.7e-34	Xylanase inhibitor C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015794)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G01300 AT3G61820
fragaria_vesca	FvH4_7g16670
malus_domestica	MD01G1082200.v1.1 MD07G1150600.v1.1
prunus_persica	Prupe.2G189400_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0359821
rosa_laevigata	RLG00000027851
rosa_roxburghii	Rroxscaffold_4G00296790
rosa_rugosa	Rorug01G0272900
rosa_samantha	Rh1AG286900 Rh1BG252700 Rh1CG269900 Rh1DG281700
rosa_wichuraiana	Rw1G025480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	504
Acc36I	ACCTGC	1 cut(s)	662
AccB1I	GGYRCC	1 cut(s)	731
AccI	GTMKAC	1 cut(s)	474
AccII	CGCG	9 cut(s)	291, 335, 442, 729, 747, 971, 1152, 1280, 1352
AccIII	TCCGGA	3 cut(s)	407, 575, 1229
AclWI	GGATC	3 cut(s)	573, 586, 1306
AcoI	YGGCCR	2 cut(s)	814, 933
AcsI	RAATTY	1 cut(s)	866
AcuI	CTGAAG	1 cut(s)	373
AcvI	CACGTG	4 cut(s)	373, 1034, 1215, 1451
AcyI	GRCGYC	5 cut(s)	237, 294, 338, 450, 501
AfaI	GTAC	5 cut(s)	219, 434, 470, 1171, 1357
AfiI	CCNNNNNNNGG	8 cut(s)	129, 383, 531, 615, 942, 1102, 1279, 1437
AflII	CTTAAG	1 cut(s)	1181
AflIII	ACRYGT	6 cut(s)	745, 987, 1031, 1033, 1212, 1214
AgeI	ACCGGT	1 cut(s)	888
AgsI	TTSAA	2 cut(s)	859, 1063
AjnI	CCWGG	2 cut(s)	464, 1419
AleI	CACNNNNGTG	4 cut(s)	737, 1112, 1259, 1453
AluBI	AGCT	3 cut(s)	396, 1006, 1335
AluI	AGCT	3 cut(s)	396, 1006, 1335
Alw21I	GWGCWC	1 cut(s)	1008
Alw26I	GTCTC	3 cut(s)	148, 409, 673
AlwI	GGATC	3 cut(s)	573, 586, 1306
AlwNI	CAGNNNCTG	1 cut(s)	1338
Ama87I	CYCGRG	2 cut(s)	1193, 1431
Aor13HI	TCCGGA	3 cut(s)	407, 575, 1229
AoxI	GGCC	5 cut(s)	778, 814, 933, 1164, 1190
ApaI	GGGCCC	1 cut(s)	1194
ApeKI	GCWGC	6 cut(s)	393, 423, 636, 1264, 1332, 1393
ApoI	RAATTY	1 cut(s)	866
ArsI	GACNNNNNNTTYG	6 cut(s)	542, 574, 1202, 1233, 1234, 1265
AsiGI	ACCGGT	1 cut(s)	888
AspLEI	GCGC	5 cut(s)	247, 291, 444, 521, 1460
AsuHPI	GGTGA	2 cut(s)	938, 949
AvaI	CYCGRG	2 cut(s)	1193, 1431
AvaII	GGWCC	6 cut(s)	132, 481, 603, 886, 891, 945
BaeGI	GKGCMC	2 cut(s)	1109, 1194
BamHI	GGATCC	1 cut(s)	578
BanI	GGYRCC	1 cut(s)	731
BanII	GRGCYC	3 cut(s)	1008, 1194, 1430
BbrPI	CACGTG	4 cut(s)	373, 1034, 1215, 1451
BbsI	GAAGAC	1 cut(s)	656
Bbv12I	GWGCWC	1 cut(s)	1008
BbvI	GCAGC	6 cut(s)	405, 435, 623, 1251, 1344, 1405
BccI	CCATC	1 cut(s)	1354
BceAI	ACGGC	2 cut(s)	920, 1065
BcgI	CGANNNNNNTGC	2 cut(s)	382, 416
BciT130I	CCWGG	2 cut(s)	466, 1421
BcoDI	GTCTC	3 cut(s)	148, 409, 673
BfaI	CTAG	1 cut(s)	881
BfoI	RGCGCY	1 cut(s)	248
BfrI	CTTAAG	1 cut(s)	1181
BfuAI	ACCTGC	1 cut(s)	662
BglI	GCCNNNNNGGC	1 cut(s)	335
BmcAI	AGTACT	1 cut(s)	434
Bme18I	GGWCC	6 cut(s)	132, 481, 603, 886, 891, 945
BmeT110I	CYCGRG	2 cut(s)	1193, 1431
BmiI	GGNNCC	9 cut(s)	512, 580, 604, 605, 685, 733, 850, 1192, 1429
BmrI	ACTGGG	1 cut(s)	1310
BmsI	GCATC	3 cut(s)	749, 1061, 1144
BmuI	ACTGGG	1 cut(s)	1310
BoxI	GACNNNNGTC	2 cut(s)	1034, 1241
BpiI	GAAGAC	1 cut(s)	656
BpmI	CTGGAG	1 cut(s)	269
BsaAI	YACGTR	5 cut(s)	373, 569, 1034, 1215, 1451
BsaHI	GRCGYC	5 cut(s)	237, 294, 338, 450, 501
BsaJI	CCNNGG	8 cut(s)	240, 333, 445, 464, 817, 1039, 1254, 1431
BsaWI	WCCGGW	6 cut(s)	407, 491, 575, 861, 888, 1229
Bsc4I	CCNNNNNNNGG	8 cut(s)	129, 383, 531, 615, 942, 1102, 1279, 1437
Bse118I	RCCGGY	3 cut(s)	845, 888, 1166
Bse1I	ACTGG	1 cut(s)	1316
BseAI	TCCGGA	3 cut(s)	407, 575, 1229
BseBI	CCWGG	2 cut(s)	466, 1421
BseDI	CCNNGG	8 cut(s)	240, 333, 445, 464, 817, 1039, 1254, 1431
BseGI	GGATG	3 cut(s)	764, 1092, 1386
BseLI	CCNNNNNNNGG	8 cut(s)	129, 383, 531, 615, 942, 1102, 1279, 1437
BseMII	CTCAG	2 cut(s)	431, 927
BseNI	ACTGG	1 cut(s)	1316
BseRI	GAGGAG	4 cut(s)	55, 80, 83, 956
BseSI	GKGCMC	2 cut(s)	1109, 1194
BseX3I	CGGCCG	2 cut(s)	814, 933
BseXI	GCAGC	6 cut(s)	405, 435, 623, 1251, 1344, 1405
Bsh1236I	CGCG	9 cut(s)	291, 335, 442, 729, 747, 971, 1152, 1280, 1352
Bsh1285I	CGRYCG	5 cut(s)	756, 817, 936, 1133, 1260
BshFI	GGCC	5 cut(s)	780, 816, 935, 1166, 1192
BshNI	GGYRCC	1 cut(s)	731
BshTI	ACCGGT	1 cut(s)	888
BsiEI	CGRYCG	5 cut(s)	756, 817, 936, 1133, 1260
BsiHKAI	GWGCWC	1 cut(s)	1008
BsiHKCI	CYCGRG	2 cut(s)	1193, 1431
BslFI	GGGAC	7 cut(s)	325, 589, 692, 756, 822, 1042, 1235
BslI	CCNNNNNNNGG	8 cut(s)	129, 383, 531, 615, 942, 1102, 1279, 1437
BsmAI	GTCTC	3 cut(s)	148, 409, 673
BsmBI	CGTCTC	2 cut(s)	148, 409
BsmFI	GGGAC	7 cut(s)	325, 589, 692, 756, 822, 1042, 1235
BsnI	GGCC	5 cut(s)	780, 816, 935, 1166, 1192
BsoBI	CYCGRG	2 cut(s)	1193, 1431
Bsp120I	GGGCCC	1 cut(s)	1190
Bsp1286I	GDGCHC	4 cut(s)	1008, 1109, 1194, 1430
Bsp13I	TCCGGA	3 cut(s)	407, 575, 1229
Bsp143I	GATC	4 cut(s)	578, 753, 1130, 1311
Bsp19I	CCATGG	1 cut(s)	240
Bsp68I	TCGCGA	1 cut(s)	971
BspANI	GGCC	5 cut(s)	780, 816, 935, 1166, 1192
BspCNI	CTCAG	2 cut(s)	430, 926
BspEI	TCCGGA	3 cut(s)	407, 575, 1229
BspFNI	CGCG	9 cut(s)	291, 335, 442, 729, 747, 971, 1152, 1280, 1352
BspLI	GGNNCC	9 cut(s)	512, 580, 604, 605, 685, 733, 850, 1192, 1429
BspMI	ACCTGC	1 cut(s)	662
BspPI	GGATC	3 cut(s)	573, 586, 1306
BspT107I	GGYRCC	1 cut(s)	731
BspTI	CTTAAG	1 cut(s)	1181
BsrFI	RCCGGY	3 cut(s)	845, 888, 1166
BsrI	ACTGG	1 cut(s)	1316
BssAI	RCCGGY	3 cut(s)	845, 888, 1166
BssECI	CCNNGG	8 cut(s)	240, 333, 445, 464, 817, 1039, 1254, 1431
BssMI	GATC	4 cut(s)	578, 753, 1130, 1311
BssNI	GRCGYC	5 cut(s)	237, 294, 338, 450, 501
BssT1I	CCWWGG	2 cut(s)	240, 445
Bst2UI	CCWGG	2 cut(s)	466, 1421
Bst4CI	ACNGT	6 cut(s)	142, 683, 694, 909, 1258, 1325
Bst6I	CTCTTC	5 cut(s)	35, 275, 788, 901, 926
BstACI	GRCGYC	5 cut(s)	237, 294, 338, 450, 501
BstAFI	CTTAAG	1 cut(s)	1181
BstAPI	GCANNNNNTGC	2 cut(s)	534, 1338
BstBAI	YACGTR	5 cut(s)	373, 569, 1034, 1215, 1451
BstC8I	GCNNGC	3 cut(s)	458, 530, 1426
BstDEI	CTNAG	2 cut(s)	417, 913
BstDSI	CCRYGG	3 cut(s)	240, 333, 1254
BstF5I	GGATG	3 cut(s)	764, 1092, 1386
BstFNI	CGCG	9 cut(s)	291, 335, 442, 729, 747, 971, 1152, 1280, 1352
BstH2I	RGCGCY	1 cut(s)	248
BstHHI	GCGC	5 cut(s)	247, 291, 444, 521, 1460
BstKTI	GATC	4 cut(s)	581, 756, 1133, 1314
BstMAI	GTCTC	3 cut(s)	148, 409, 673
BstMBI	GATC	4 cut(s)	578, 753, 1130, 1311
BstMCI	CGRYCG	5 cut(s)	756, 817, 936, 1133, 1260
BstMWI	GCNNNNNNNGC	9 cut(s)	288, 332, 335, 393, 525, 534, 1142, 1163, 1338
BstNI	CCWGG	2 cut(s)	466, 1421
BstPAI	GACNNNNGTC	2 cut(s)	1034, 1241
BstSLI	GKGCMC	2 cut(s)	1109, 1194
BstUI	CGCG	9 cut(s)	291, 335, 442, 729, 747, 971, 1152, 1280, 1352
BstV1I	GCAGC	6 cut(s)	405, 435, 623, 1251, 1344, 1405
BstV2I	GAAGAC	1 cut(s)	656
BstX2I	RGATCY	1 cut(s)	578
BstYI	RGATCY	1 cut(s)	578
BstZI	CGGCCG	2 cut(s)	814, 933
BsuRI	GGCC	5 cut(s)	780, 816, 935, 1166, 1192
BtgI	CCRYGG	3 cut(s)	240, 333, 1254
BtgZI	GCGATG	2 cut(s)	1038, 1167
BtsCI	GGATG	3 cut(s)	764, 1092, 1386
BtsIMutI	CAGTG	3 cut(s)	690, 1024, 1323
BtuMI	TCGCGA	1 cut(s)	971
BveI	ACCTGC	1 cut(s)	662
Cac8I	GCNNGC	3 cut(s)	458, 530, 1426
CaiI	CAGNNNCTG	1 cut(s)	1338
CfoI	GCGC	5 cut(s)	247, 291, 444, 521, 1460
Cfr10I	RCCGGY	3 cut(s)	845, 888, 1166
Cfr42I	CCGCGG	1 cut(s)	336
Cfr9I	CCCGGG	1 cut(s)	1431
CpoI	CGGWCCG	1 cut(s)	945
CseI	GACGC	5 cut(s)	245, 302, 327, 439, 753
Csp6I	GTAC	5 cut(s)	218, 433, 469, 1170, 1356
CspAI	ACCGGT	1 cut(s)	888
CspI	CGGWCCG	1 cut(s)	945
CviAII	CATG	3 cut(s)	129, 241, 478
CviQI	GTAC	5 cut(s)	218, 433, 469, 1170, 1356
DdeI	CTNAG	2 cut(s)	417, 913
DpnI	GATC	4 cut(s)	580, 755, 1132, 1313
DpnII	GATC	4 cut(s)	578, 753, 1130, 1311
EaeI	YGGCCR	2 cut(s)	814, 933
EagI	CGGCCG	2 cut(s)	814, 933
Eam1104I	CTCTTC	5 cut(s)	35, 275, 788, 901, 926
EarI	CTCTTC	5 cut(s)	35, 275, 788, 901, 926
EciI	GGCGGA	5 cut(s)	34, 58, 148, 175, 269
Ecl136II	GAGCTC	1 cut(s)	1006
EclXI	CGGCCG	2 cut(s)	814, 933
Eco130I	CCWWGG	2 cut(s)	240, 445
Eco24I	GRGCYC	3 cut(s)	1008, 1194, 1430
Eco47I	GGWCC	6 cut(s)	132, 481, 603, 886, 891, 945
Eco52I	CGGCCG	2 cut(s)	814, 933
Eco53kI	GAGCTC	1 cut(s)	1006
Eco57I	CTGAAG	1 cut(s)	373
Eco72I	CACGTG	4 cut(s)	373, 1034, 1215, 1451
Eco88I	CYCGRG	2 cut(s)	1193, 1431
EcoICRI	GAGCTC	1 cut(s)	1006
EcoO109I	RGGNCCY	2 cut(s)	603, 778
EcoRII	CCWGG	2 cut(s)	464, 1419
EcoT14I	CCWWGG	2 cut(s)	240, 445
EcoT38I	GRGCYC	3 cut(s)	1008, 1194, 1430
ErhI	CCWWGG	2 cut(s)	240, 445
Esp3I	CGTCTC	2 cut(s)	148, 409
FaeI	CATG	3 cut(s)	132, 244, 481
FaiI	YATR	5 cut(s)	130, 242, 479, 1376, 1416
FalI	AAGNNNNNCTT	1 cut(s)	35
FaqI	GGGAC	7 cut(s)	325, 589, 692, 756, 822, 1042, 1235
FatI	CATG	3 cut(s)	128, 240, 477
FauI	CCCGC	5 cut(s)	790, 844, 1345, 1358, 1417
FblI	GTMKAC	1 cut(s)	474
FokI	GGATG	3 cut(s)	771, 1079, 1373
FriOI	GRGCYC	3 cut(s)	1008, 1194, 1430
FspBI	CTAG	1 cut(s)	881
GlaI	GCGC	5 cut(s)	246, 290, 443, 520, 1459
GsuI	CTGGAG	1 cut(s)	269
HaeII	RGCGCY	1 cut(s)	248
HaeIII	GGCC	5 cut(s)	780, 816, 935, 1166, 1192
HgaI	GACGC	5 cut(s)	245, 302, 327, 439, 753
HhaI	GCGC	5 cut(s)	247, 291, 444, 521, 1460
Hin1I	GRCGYC	5 cut(s)	237, 294, 338, 450, 501
Hin1II	CATG	3 cut(s)	132, 244, 481
Hin6I	GCGC	5 cut(s)	245, 289, 442, 519, 1458
HinP1I	GCGC	5 cut(s)	245, 289, 442, 519, 1458
HinfI	GANTC	7 cut(s)	149, 155, 411, 626, 810, 929, 1100
HphI	GGTGA	2 cut(s)	938, 949
Hpy166II	GTNNAC	4 cut(s)	475, 886, 957, 1321
Hpy188I	TCNGA	5 cut(s)	136, 148, 154, 758, 1274
Hpy188III	TCNNGA	6 cut(s)	408, 576, 767, 970, 1067, 1230
Hpy8I	GTNNAC	4 cut(s)	475, 886, 957, 1321
Hpy99I	CGWCG	8 cut(s)	239, 296, 404, 455, 503, 506, 791, 1004
HpyAV	CCTTC	4 cut(s)	35, 307, 592, 697
HpyCH4III	ACNGT	6 cut(s)	142, 683, 694, 909, 1258, 1325
HpyCH4V	TGCA	3 cut(s)	528, 639, 1267
HpyF10VI	GCNNNNNNNGC	9 cut(s)	288, 332, 335, 393, 525, 534, 1142, 1163, 1338
HpyF3I	CTNAG	2 cut(s)	417, 913
Hsp92I	GRCGYC	5 cut(s)	237, 294, 338, 450, 501
Hsp92II	CATG	3 cut(s)	132, 244, 481
HspAI	GCGC	5 cut(s)	245, 289, 442, 519, 1458
KflI	GGGWCCC	1 cut(s)	603
Kpn2I	TCCGGA	3 cut(s)	407, 575, 1229
KspI	CCGCGG	1 cut(s)	336
Kzo9I	GATC	4 cut(s)	578, 753, 1130, 1311
LmnI	GCTCC	4 cut(s)	401, 516, 854, 1433
Lsp1109I	GCAGC	6 cut(s)	405, 435, 623, 1251, 1344, 1405
LweI	GCATC	3 cut(s)	749, 1061, 1144
MaeI	CTAG	1 cut(s)	881
MaeIII	GTNAC	6 cut(s)	368, 379, 764, 926, 1114, 1382
MalI	GATC	4 cut(s)	580, 755, 1132, 1313
MboI	GATC	4 cut(s)	578, 753, 1130, 1311
MboII	GAAGA	7 cut(s)	22, 262, 661, 775, 888, 891, 913
MflI	RGATCY	1 cut(s)	578
MhlI	GDGCHC	4 cut(s)	1008, 1109, 1194, 1430
MluCI	AATT	5 cut(s)	221, 640, 854, 866, 1377
MluI	ACGCGT	1 cut(s)	745
MlyI	GAGTC	4 cut(s)	405, 620, 804, 923
MmeI	TCCRAC	1 cut(s)	159
MroI	TCCGGA	3 cut(s)	407, 575, 1229
MseI	TTAA	3 cut(s)	249, 1182, 1462
MslI	CAYNNNNRTG	4 cut(s)	737, 1112, 1259, 1453
MspA1I	CMGCKG	2 cut(s)	335, 426
MspCI	CTTAAG	1 cut(s)	1181
MvaI	CCWGG	2 cut(s)	466, 1421
MvnI	CGCG	9 cut(s)	291, 335, 442, 729, 747, 971, 1152, 1280, 1352
MwoI	GCNNNNNNNGC	9 cut(s)	288, 332, 335, 393, 525, 534, 1142, 1163, 1338
NcoI	CCATGG	1 cut(s)	240
NdeII	GATC	4 cut(s)	578, 753, 1130, 1311
NlaIII	CATG	3 cut(s)	132, 244, 481
NlaIV	GGNNCC	9 cut(s)	512, 580, 604, 605, 685, 733, 850, 1192, 1429
NmeAIII	GCCGAG	2 cut(s)	792, 911
NmuCI	GTSAC	5 cut(s)	368, 379, 764, 926, 1114
NruI	TCGCGA	1 cut(s)	971
OliI	CACNNNNGTG	4 cut(s)	737, 1112, 1259, 1453
PfeI	GAWTC	3 cut(s)	149, 155, 1100
PflFI	GACNNNGTC	1 cut(s)	140
PfoI	TCCNGGA	1 cut(s)	941
PinAI	ACCGGT	1 cut(s)	888
Ple19I	CGATCG	2 cut(s)	756, 1133
PleI	GAGTC	4 cut(s)	405, 620, 804, 923
PmaCI	CACGTG	4 cut(s)	373, 1034, 1215, 1451
PmlI	CACGTG	4 cut(s)	373, 1034, 1215, 1451
PpsI	GAGTC	4 cut(s)	405, 620, 804, 923
Ppu21I	YACGTR	5 cut(s)	373, 569, 1034, 1215, 1451
PpuMI	RGGWCCY	1 cut(s)	603
PshAI	GACNNNNGTC	2 cut(s)	1034, 1241
Psp124BI	GAGCTC	1 cut(s)	1008
Psp5II	RGGWCCY	1 cut(s)	603
Psp6I	CCWGG	2 cut(s)	464, 1419
PspCI	CACGTG	4 cut(s)	373, 1034, 1215, 1451
PspGI	CCWGG	2 cut(s)	464, 1419
PspN4I	GGNNCC	9 cut(s)	512, 580, 604, 605, 685, 733, 850, 1192, 1429
PspOMI	GGGCCC	1 cut(s)	1190
PspPPI	RGGWCCY	1 cut(s)	603
PstNI	CAGNNNCTG	1 cut(s)	1338
PsuI	RGATCY	1 cut(s)	578
PsyI	GACNNNGTC	1 cut(s)	140
PvuI	CGATCG	2 cut(s)	756, 1133
RruI	TCGCGA	1 cut(s)	971
RsaI	GTAC	5 cut(s)	219, 434, 470, 1171, 1357
RsaNI	GTAC	5 cut(s)	218, 433, 469, 1170, 1356
RseI	CAYNNNNRTG	4 cut(s)	737, 1112, 1259, 1453
Rsr2I	CGGWCCG	1 cut(s)	945
RsrII	CGGWCCG	1 cut(s)	945
SacI	GAGCTC	1 cut(s)	1008
SacII	CCGCGG	1 cut(s)	336
SaqAI	TTAA	3 cut(s)	249, 1182, 1462
Sau3AI	GATC	4 cut(s)	578, 753, 1130, 1311
ScaI	AGTACT	1 cut(s)	434
SchI	GAGTC	4 cut(s)	405, 620, 804, 923
SduI	GDGCHC	4 cut(s)	1008, 1109, 1194, 1430
SfaNI	GCATC	3 cut(s)	749, 1061, 1144
Sfr303I	CCGCGG	1 cut(s)	336
SgrBI	CCGCGG	1 cut(s)	336
SinI	GGWCC	6 cut(s)	132, 481, 603, 886, 891, 945
SmaI	CCCGGG	1 cut(s)	1433
SmiMI	CAYNNNNRTG	4 cut(s)	737, 1112, 1259, 1453
SmlI	CTYRAG	1 cut(s)	1181
SmoI	CTYRAG	1 cut(s)	1181
Sse9I	AATT	5 cut(s)	221, 640, 854, 866, 1377
SspMI	CTAG	1 cut(s)	881
SstI	GAGCTC	1 cut(s)	1008
StyI	CCWWGG	2 cut(s)	240, 445
TaaI	ACNGT	6 cut(s)	142, 683, 694, 909, 1258, 1325
TaqII	GACCGA	2 cut(s)	563, 1253
TasI	AATT	5 cut(s)	221, 640, 854, 866, 1377
TatI	WGTACW	1 cut(s)	432
TauI	GCSGC	8 cut(s)	329, 332, 335, 338, 429, 732, 797, 1332
TfiI	GAWTC	3 cut(s)	149, 155, 1100
Tru1I	TTAA	3 cut(s)	249, 1182, 1462
Tru9I	TTAA	3 cut(s)	249, 1182, 1462
TscAI	CASTG	3 cut(s)	697, 1024, 1323
TseFI	GTSAC	5 cut(s)	368, 379, 764, 926, 1114
TseI	GCWGC	6 cut(s)	393, 423, 636, 1264, 1332, 1393
Tsp45I	GTSAC	5 cut(s)	368, 379, 764, 926, 1114
TspGWI	ACGGA	5 cut(s)	205, 290, 388, 1098, 1102
TspMI	CCCGGG	1 cut(s)	1431
TspRI	CASTG	3 cut(s)	697, 1024, 1323
Tth111I	GACNNNGTC	1 cut(s)	140
Vha464I	CTTAAG	1 cut(s)	1181
VpaK11BI	GGWCC	6 cut(s)	132, 481, 603, 886, 891, 945
XapI	RAATTY	1 cut(s)	866
XmaI	CCCGGG	1 cut(s)	1431
XmiI	GTMKAC	1 cut(s)	474
XspI	CTAG	1 cut(s)	881
ZraI	GACGTC	1 cut(s)	502
ZrmI	AGTACT	1 cut(s)	434

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.