Rorug01G0272900

Cyclic pyranopterin monophosphate synthase accessory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0012432
Physical Location & Seq
Reverse (-)
38547096 .. 38549998
2903 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0272900.1

Sequence Viewer

Length: 1305 bp
ATGGATCGAAGGCAAATTATGTTGCTCTTATTGGTGGAGTTGTGGCACCTAGAGACAATGTGTGCTTGTACTATGCTTGTGCTACTGATGTTTAATTTGAGACGAAGACGTGCTGAAAGACATGCTCTAAATAATCGTTCATTCGTTAGACGTGAGACTCGTTTGAGTTATCTAGATAGTATTATAGGTAATAGTGACACTGAATGTGTACACGAATTAAGAATGGATAGAAGGGCTTTTGGCCTTTTGTGTGAACTGCTTCGCACAGATGGAAAGATAAAGAGTGATGGTTTGGTGTCTGTGGAAGAGCAAGTATGTATGTTTTTACATATACTTTCACATCATGTGAAGAATCGTACTATTAGAGGTAGATTTTTTCGATCTGGAGAGACTATTAGTAGGTATTTCCATTCTATACTACAAGGTGTTTTACGATTGCAAGGTAGTTTGTTGAGGGTACCTGATCCGGTACTTGCTGACTGCACAGATCGTAGATGGAAGTGCTTTAAGAATTGCTTGGGGGCACTAGATGGAACTTACATTAGAGTGCGTGTACCTGCAATTGACAAGCCAAGATACCGTACAAGAAAGGGTGAAGTTGCTACAAATGTGTTAGCTGCTTGTTCTCGTGATATGCAGTTCATATTTGTCTTACCGGGTTGGGAGGGATCGGCATCGGACTCTAGAGTTCTTCACGATGCAGTGACTAGGCCGAATGGATTAAGAGTTCCCACTGGTTATTATTACCTTGTAGATGGGGGTTACACAAATGGTGAAGGATTTCTTGCACCATATAGAGGAACAAGGTATCATTTATCAGAATGGGATGAAAGAAATATGCCTAGGGATCATGTGGAATATTTTAACATGAAGCATTCGAAGGCAAGAAATGTCATAGAACGGTGTTTTAGAATGCTAAAAGGAAGGTGGGGAATACTAAGGAGTCCATCATTTTATCCAATAAAGACACAATGCCGCATAATTACGGTTTGTTGTCTTTTACATAATTTTATTAGAAGAGAAATGTCCATTGATCCGATGGAGTATGCAGTATGTGAATTAGATGCTCAAAATGGGGAAGAAGTTGATGTAGTTGGCACGGTTGAAGCGTCTAATCAATGGACTACTTGGAGGAATGAATTAGCTTCACAAATGCATAATGAATGGACGGGAAGAAGGGTTGTAAGGGCTGCCGGAGATACAGGGGTTTCAGGTGCTGCTGCAAGGGCTGCAAGGGATGCAAGAGCTGCAAGGGATGCAAGGGATGCAAGGGCTGCAAGGGATGCAAGAGCTTCAAGAGAATAA

Protein Analysis

434

Amino Acids

50.05

Weight (kDa)

9.53

Isoelectric Point (pI)

47.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 56 - 144 6.8e-24 Domain of unknown function (DUF8040)
DDE_Tnp_1 PF01609 172 - 334 2.3e-10 Transposase DDE domain
DDE_Tnp_4 PF13359 176 - 336 6e-28 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012432)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01290 AT1G01290
fragaria_vesca FvH4_7g16680
malus_domestica MD07G1150800.v1.1
prunus_persica Prupe.2G189500_v2.0.a1 Prupe.2G189500_v2.0.a1 Prupe.2G189500_v2.0.a1
pyrus_communis pycom07g14910
rosa_chinensis RchiOBHm_Chr1g0359831
rosa_laevigata RLG00000027850
rosa_multiflora Rmu_sc0003191.1_g000005 Rmu_ssc0000371.1_g000002
rosa_roxburghii Rroxscaffold_4G00296780
rosa_rugosa Rorug01G0272900
rosa_samantha Rh1AG287000 Rh1BG252800 Rh1CG270000 Rh1DG281800
rosa_wichuraiana Rw1G025490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 565
Acc65I GGTACC 1 cut(s) 457
AccB1I GGYRCC 2 cut(s) 45, 457
AciI CCGC 1 cut(s) 976
AclWI GGATC 5 cut(s) 12, 458, 676, 855, 1028
AfaI GTAC 7 cut(s) 70, 210, 358, 459, 471, 555, 583
AfiI CCNNNNNNNGG 1 cut(s) 797
AgsI TTSAA 2 cut(s) 1106, 1296
AjiI CACGTC 2 cut(s) 110, 152
AloI GAACNNNNNNTCC 2 cut(s) 711, 743
AluBI AGCT 4 cut(s) 617, 1145, 1247, 1292
AluI AGCT 4 cut(s) 617, 1145, 1247, 1292
Alw26I GTCTC 4 cut(s) 47, 94, 149, 383
AlwI GGATC 5 cut(s) 12, 458, 676, 855, 1028
AlwNI CAGNNNCTG 1 cut(s) 1217
AoxI GGCC 2 cut(s) 241, 710
ApeKI GCWGC 7 cut(s) 617, 1190, 1217, 1220, 1229, 1247, 1274
Asp700I GAANNNNTTC 2 cut(s) 258, 780
Asp718I GGTACC 1 cut(s) 457
AspA2I CCTAGG 1 cut(s) 842
AsuC2I CCSGG 1 cut(s) 657
AsuHPI GGTGA 2 cut(s) 605, 785
AsuII TTCGAA 1 cut(s) 878
AvrII CCTAGG 1 cut(s) 842
BaeGI GKGCMC 1 cut(s) 526
BaeI ACNNNNGTAYC 2 cut(s) 1191, 1224
BanI GGYRCC 2 cut(s) 45, 457
BauI CACGAG 1 cut(s) 627
BbsI GAAGAC 1 cut(s) 112
BbvI GCAGC 7 cut(s) 604, 1177, 1204, 1207, 1216, 1234, 1261
BccI CCATC 7 cut(s) 263, 281, 489, 524, 749, 955, 1033
BcnI CCSGG 1 cut(s) 657
BcoDI GTCTC 4 cut(s) 47, 94, 149, 383
BfaI CTAG 6 cut(s) 50, 173, 527, 684, 708, 843
BfuAI ACCTGC 1 cut(s) 565
BisI GCNGC 8 cut(s) 618, 976, 1191, 1218, 1221, 1230, 1248, 1275
BlnI CCTAGG 1 cut(s) 842
BlsI GCNGC 8 cut(s) 619, 977, 1192, 1219, 1222, 1231, 1249, 1276
Bme1390I CCNGG 1 cut(s) 657
BmgBI CACGTC 2 cut(s) 110, 152
BmiI GGNNCC 2 cut(s) 47, 459
BmrFI CCNGG 1 cut(s) 657
BmsI GCATC 7 cut(s) 683, 688, 1054, 1228, 1246, 1255, 1273
BpiI GAAGAC 1 cut(s) 112
BpmI CTGGAG 1 cut(s) 405
Bpu14I TTCGAA 1 cut(s) 878
BpuMI CCSGG 1 cut(s) 657
BsaBI GATNNNNATC 1 cut(s) 673
BsaJI CCNNGG 1 cut(s) 842
BsaWI WCCGGW 1 cut(s) 466
Bsc4I CCNNNNNNNGG 1 cut(s) 797
Bse1I ACTGG 1 cut(s) 739
Bse8I GATNNNNATC 1 cut(s) 673
BseDI CCNNGG 1 cut(s) 842
BseGI GGATG 5 cut(s) 832, 1243, 1261, 1270, 1288
BseJI GATNNNNATC 1 cut(s) 673
BseLI CCNNNNNNNGG 1 cut(s) 797
BseNI ACTGG 1 cut(s) 739
BseSI GKGCMC 1 cut(s) 526
BseXI GCAGC 7 cut(s) 604, 1177, 1204, 1207, 1216, 1234, 1261
BsgI GTGCAG 1 cut(s) 466
BshFI GGCC 2 cut(s) 243, 712
BshNI GGYRCC 2 cut(s) 45, 457
BsiSI CCGG 3 cut(s) 467, 656, 1194
BslI CCNNNNNNNGG 1 cut(s) 797
BsmAI GTCTC 4 cut(s) 47, 94, 149, 383
BsmBI CGTCTC 1 cut(s) 94
BsmI GAATGC 2 cut(s) 874, 918
BsnI GGCC 2 cut(s) 243, 712
Bsp119I TTCGAA 1 cut(s) 878
Bsp1286I GDGCHC 1 cut(s) 526
Bsp1407I TGTACA 1 cut(s) 208
Bsp143I GATC 7 cut(s) 4, 380, 463, 487, 668, 847, 1033
BspACI CCGC 1 cut(s) 976
BspANI GGCC 2 cut(s) 243, 712
BspLI GGNNCC 2 cut(s) 47, 459
BspMI ACCTGC 1 cut(s) 565
BspPI GGATC 5 cut(s) 12, 458, 676, 855, 1028
BspQI GCTCTTC 1 cut(s) 300
BspT104I TTCGAA 1 cut(s) 878
BspT107I GGYRCC 2 cut(s) 45, 457
BsrGI TGTACA 1 cut(s) 208
BsrI ACTGG 1 cut(s) 739
BssECI CCNNGG 1 cut(s) 842
BssMI GATC 7 cut(s) 4, 380, 463, 487, 668, 847, 1033
BssSI CACGAG 1 cut(s) 627
BssT1I CCWWGG 1 cut(s) 842
Bst2BI CACGAG 1 cut(s) 627
Bst4CI ACNGT 4 cut(s) 581, 903, 988, 1102
Bst6I CTCTTC 2 cut(s) 300, 1012
BstAPI GCANNNNNTGC 7 cut(s) 1229, 1238, 1247, 1256, 1265, 1274, 1283
BstAUI TGTACA 1 cut(s) 208
BstBI TTCGAA 1 cut(s) 878
BstDEI CTNAG 1 cut(s) 938
BstF5I GGATG 5 cut(s) 832, 1243, 1261, 1270, 1288
BstKTI GATC 7 cut(s) 7, 383, 466, 490, 671, 850, 1036
BstMAI GTCTC 4 cut(s) 47, 94, 149, 383
BstMBI GATC 7 cut(s) 4, 380, 463, 487, 668, 847, 1033
BstMWI GCNNNNNNNGC 8 cut(s) 1226, 1229, 1238, 1247, 1256, 1265, 1274, 1283
BstNSI RCATGY 1 cut(s) 125
BstSCI CCNGG 1 cut(s) 655
BstSLI GKGCMC 1 cut(s) 526
BstV1I GCAGC 7 cut(s) 604, 1177, 1204, 1207, 1216, 1234, 1261
BstV2I GAAGAC 1 cut(s) 112
BsuRI GGCC 2 cut(s) 243, 712
BtrI CACGTC 2 cut(s) 110, 152
BtsCI GGATG 5 cut(s) 832, 1243, 1261, 1270, 1288
BtsI GCAGTG 1 cut(s) 708
BtsIMutI CAGTG 3 cut(s) 198, 708, 732
BveI ACCTGC 1 cut(s) 565
CaiI CAGNNNCTG 1 cut(s) 1217
CseI GACGC 1 cut(s) 1098
Csp6I GTAC 7 cut(s) 69, 209, 357, 458, 470, 554, 582
CviAII CATG 4 cut(s) 122, 344, 851, 868
CviQI GTAC 7 cut(s) 69, 209, 357, 458, 470, 554, 582
DdeI CTNAG 1 cut(s) 938
DpnI GATC 7 cut(s) 6, 382, 465, 489, 670, 849, 1035
DpnII GATC 7 cut(s) 4, 380, 463, 487, 668, 847, 1033
Eam1104I CTCTTC 2 cut(s) 300, 1012
EarI CTCTTC 2 cut(s) 300, 1012
Eco130I CCWWGG 1 cut(s) 842
EcoT14I CCWWGG 1 cut(s) 842
EcoT22I ATGCAT 1 cut(s) 1158
ErhI CCWWGG 1 cut(s) 842
Esp3I CGTCTC 1 cut(s) 94
FaeI CATG 4 cut(s) 125, 347, 854, 871
FalI AAGNNNNNCTT 4 cut(s) 500, 532, 768, 800
FatI CATG 4 cut(s) 121, 343, 850, 867
Fnu4HI GCNGC 8 cut(s) 618, 976, 1191, 1218, 1221, 1230, 1248, 1275
FokI GGATG 5 cut(s) 839, 1250, 1268, 1277, 1295
Fsp4HI GCNGC 8 cut(s) 618, 976, 1191, 1218, 1221, 1230, 1248, 1275
FspBI CTAG 6 cut(s) 50, 173, 527, 684, 708, 843
GluI GCNGC 8 cut(s) 618, 976, 1191, 1218, 1221, 1230, 1248, 1275
GsuI CTGGAG 1 cut(s) 405
HaeIII GGCC 2 cut(s) 243, 712
HapII CCGG 3 cut(s) 467, 656, 1194
HgaI GACGC 1 cut(s) 1098
Hin1II CATG 4 cut(s) 125, 347, 854, 871
HinfI GANTC 4 cut(s) 157, 352, 680, 943
HpaII CCGG 3 cut(s) 467, 656, 1194
HphI GGTGA 2 cut(s) 605, 785
Hpy166II GTNNAC 4 cut(s) 209, 211, 254, 554
Hpy188I TCNGA 3 cut(s) 679, 820, 1038
Hpy188III TCNNGA 6 cut(s) 173, 384, 629, 684, 695, 1296
Hpy8I GTNNAC 4 cut(s) 209, 211, 254, 554
HpyAV CCTTC 6 cut(s) 3, 225, 770, 874, 918, 1170
HpyCH4III ACNGT 4 cut(s) 581, 903, 988, 1102
HpyCH4IV ACGT 2 cut(s) 109, 151
HpyF10VI GCNNNNNNNGC 8 cut(s) 1226, 1229, 1238, 1247, 1256, 1265, 1274, 1283
HpyF3I CTNAG 1 cut(s) 938
HpySE526I ACGT 2 cut(s) 109, 151
Hsp92II CATG 4 cut(s) 125, 347, 854, 871
KpnI GGTACC 1 cut(s) 461
Kzo9I GATC 7 cut(s) 4, 380, 463, 487, 668, 847, 1033
LguI GCTCTTC 1 cut(s) 300
LpnPI CCDG 9 cut(s) 369, 474, 480, 570, 669, 720, 1188, 1197, 1207
Lsp1109I GCAGC 7 cut(s) 604, 1177, 1204, 1207, 1216, 1234, 1261
LweI GCATC 7 cut(s) 683, 688, 1054, 1228, 1246, 1255, 1273
MaeI CTAG 6 cut(s) 50, 173, 527, 684, 708, 843
MaeII ACGT 2 cut(s) 109, 151
MaeIII GTNAC 3 cut(s) 194, 703, 761
MalI GATC 7 cut(s) 6, 382, 465, 489, 670, 849, 1035
MboI GATC 7 cut(s) 4, 380, 463, 487, 668, 847, 1033
MboII GAAGA 7 cut(s) 117, 317, 361, 683, 1029, 1091, 1185
MfeI CAATTG 1 cut(s) 561
MhlI GDGCHC 1 cut(s) 526
MluCI AATT 9 cut(s) 15, 94, 215, 511, 561, 981, 1006, 1058, 1139
MlyI GAGTC 3 cut(s) 151, 674, 952
MnlI CCTC 5 cut(s) 359, 447, 658, 791, 1125
Mph1103I ATGCAT 1 cut(s) 1158
MroXI GAANNNNTTC 2 cut(s) 258, 780
MseI TTAA 5 cut(s) 93, 218, 507, 722, 864
MslI CAYNNNNRTG 1 cut(s) 545
MspI CCGG 3 cut(s) 467, 656, 1194
MspR9I CCNGG 1 cut(s) 657
MunI CAATTG 1 cut(s) 561
Mva1269I GAATGC 2 cut(s) 874, 918
MwoI GCNNNNNNNGC 8 cut(s) 1226, 1229, 1238, 1247, 1256, 1265, 1274, 1283
NciI CCSGG 1 cut(s) 657
NdeII GATC 7 cut(s) 4, 380, 463, 487, 668, 847, 1033
NlaIII CATG 4 cut(s) 125, 347, 854, 871
NlaIV GGNNCC 2 cut(s) 47, 459
NmuCI GTSAC 2 cut(s) 194, 703
NsiI ATGCAT 1 cut(s) 1158
NspI RCATGY 1 cut(s) 125
NspV TTCGAA 1 cut(s) 878
PciSI GCTCTTC 1 cut(s) 300
PcsI WCGNNNNNNNCGW 2 cut(s) 157, 1106
PctI GAATGC 2 cut(s) 874, 918
PdmI GAANNNNTTC 2 cut(s) 258, 780
PfeI GAWTC 1 cut(s) 352
PkrI GCNGC 8 cut(s) 619, 977, 1192, 1219, 1222, 1231, 1249, 1276
PleI GAGTC 3 cut(s) 151, 674, 951
PpsI GAGTC 3 cut(s) 151, 674, 951
PspN4I GGNNCC 2 cut(s) 47, 459
PstNI CAGNNNCTG 1 cut(s) 1217
RsaI GTAC 7 cut(s) 70, 210, 358, 459, 471, 555, 583
RsaNI GTAC 7 cut(s) 69, 209, 357, 458, 470, 554, 582
RseI CAYNNNNRTG 1 cut(s) 545
SapI GCTCTTC 1 cut(s) 300
SaqAI TTAA 5 cut(s) 93, 218, 507, 722, 864
SatI GCNGC 8 cut(s) 618, 976, 1191, 1218, 1221, 1230, 1248, 1275
Sau3AI GATC 7 cut(s) 4, 380, 463, 487, 668, 847, 1033
SchI GAGTC 3 cut(s) 151, 674, 952
ScrFI CCNGG 1 cut(s) 657
SduI GDGCHC 1 cut(s) 526
SfaNI GCATC 7 cut(s) 683, 688, 1054, 1228, 1246, 1255, 1273
SfuI TTCGAA 1 cut(s) 878
SmiMI CAYNNNNRTG 1 cut(s) 545
Sse9I AATT 9 cut(s) 15, 94, 215, 511, 561, 981, 1006, 1058, 1139
SsiI CCGC 1 cut(s) 976
SspI AATATT 1 cut(s) 860
SspMI CTAG 6 cut(s) 50, 173, 527, 684, 708, 843
StyD4I CCNGG 1 cut(s) 655
StyI CCWWGG 1 cut(s) 842
TaaI ACNGT 4 cut(s) 581, 903, 988, 1102
TaiI ACGT 2 cut(s) 112, 154
TaqI TCGA 3 cut(s) 7, 379, 878
TasI AATT 9 cut(s) 15, 94, 215, 511, 561, 981, 1006, 1058, 1139
TatI WGTACW 2 cut(s) 68, 208
TauI GCSGC 1 cut(s) 978
TfiI GAWTC 1 cut(s) 352
Tru1I TTAA 5 cut(s) 93, 218, 507, 722, 864
Tru9I TTAA 5 cut(s) 93, 218, 507, 722, 864
TscAI CASTG 3 cut(s) 205, 708, 739
TseFI GTSAC 2 cut(s) 194, 703
TseI GCWGC 7 cut(s) 617, 1190, 1217, 1220, 1229, 1247, 1274
Tsp45I GTSAC 2 cut(s) 194, 703
TspDTI ATGAA 6 cut(s) 129, 631, 843, 884, 1152, 1176
TspRI CASTG 3 cut(s) 205, 708, 739
XbaI TCTAGA 2 cut(s) 172, 683
XceI RCATGY 1 cut(s) 125
XcmI CCANNNNNNNNNTGG 1 cut(s) 1036
XmaJI CCTAGG 1 cut(s) 842
XmnI GAANNNNTTC 2 cut(s) 258, 780
XspI CTAG 6 cut(s) 50, 173, 527, 684, 708, 843
Zsp2I ATGCAT 1 cut(s) 1158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.