FvH4_7g16680

Cyclic pyranopterin monophosphate synthase accessory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0012432
Physical Location & Seq
Reverse (-)
14251511 .. 14252356
846 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g16680.t1

Sequence Viewer

Length: 702 bp
ATGTTTCTTCGGCGAATGGCGGTTTCGCTTTCTCACTCCAGAAGGTTTCTTAGCAGTAGCAGCAACAGCAGCATTGACATTGCGAAAGCAATTGATGAGCTTAACAAGGAAATGGAAGCAGTGTTTGGTGAACCTCCAGCAGATAGCGGGCTTGCCAGTTCTGCAAGAAATGATGTTATGGCTCAAGAACCCGAGTTAGTATCACGAGAAAAAGGAGAAGACATGCATGGCCTAACCCACATTGGCGCTACGGGGGAGGCTCAGATGGTGGATGTTTCTCCAAAACCAAGTACCAAGAGAACTGCCGTTTCTAGTTGCAAGGTACTTCTTGGCAAGCAGGTGTTTGATTTGGTGTTAGCCAACCAGATGGCTAAGGGAGATGTGCTTAGTGTGGCCAAGATTGCTGGAATAAATGGAGCAAAGCACACAAGCAATCTCATCCCGCTATGCCACAACATACCTCTGACCCATGTCCGCGTTGATCTGACACTAAATCCCAAGGATTTCAGTGTGGATATCGAAGCAGAAGCTAGTTCAACCGGGAAAACTGGGGTTGAAATGGAAGCCATGACAGCTGCAAGTGTTGCTGGCCTAACCGTCTATGATATGTGCAAAGCTGCTTCGAAGTATATACAGATCACTGATATAAGACTTGAGAGTAAGGCTGGTGGGAAGAGTGGAGACTGGCACAGGGAGGAGTAA

Protein Analysis

234

Amino Acids

24.93

Weight (kDa)

5.99

Isoelectric Point (pI)

43.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MoaC PF01967 89 - 224 2e-53 MoaC family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012432)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01290 AT1G01290
fragaria_vesca FvH4_7g16680
malus_domestica MD07G1150800.v1.1
prunus_persica Prupe.2G189500_v2.0.a1 Prupe.2G189500_v2.0.a1 Prupe.2G189500_v2.0.a1
pyrus_communis pycom07g14910
rosa_chinensis RchiOBHm_Chr1g0359831
rosa_laevigata RLG00000027850
rosa_multiflora Rmu_sc0003191.1_g000005 Rmu_ssc0000371.1_g000002
rosa_roxburghii Rroxscaffold_4G00296780
rosa_rugosa Rorug01G0272900
rosa_samantha Rh1AG287000 Rh1BG252800 Rh1CG270000 Rh1DG281800
rosa_wichuraiana Rw1G025490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 328
Acc36I ACCTGC 1 cut(s) 328
AccII CGCG 1 cut(s) 477
AciI CCGC 4 cut(s) 20, 147, 443, 475
AcoI YGGCCR 1 cut(s) 393
AfaI GTAC 2 cut(s) 292, 324
AgsI TTSAA 2 cut(s) 537, 557
AjuI GAANNNNNNNTTGG 2 cut(s) 108, 140
AluBI AGCT 4 cut(s) 100, 530, 575, 617
AluI AGCT 4 cut(s) 100, 530, 575, 617
Alw26I GTCTC 1 cut(s) 675
Ama87I CYCGRG 1 cut(s) 191
AoxI GGCC 3 cut(s) 229, 393, 589
ApeKI GCWGC 4 cut(s) 60, 69, 575, 617
AspLEI GCGC 1 cut(s) 248
AsuC2I CCSGG 1 cut(s) 541
AsuHPI GGTGA 1 cut(s) 140
AsuII TTCGAA 1 cut(s) 623
AvaI CYCGRG 1 cut(s) 191
BalI TGGCCA 1 cut(s) 395
BauI CACGAG 1 cut(s) 204
BbsI GAAGAC 1 cut(s) 225
BbvI GCAGC 4 cut(s) 72, 81, 562, 604
BccI CCATC 2 cut(s) 259, 361
BceAI ACGGC 1 cut(s) 290
BcnI CCSGG 1 cut(s) 541
BcoDI GTCTC 1 cut(s) 675
BfaI CTAG 2 cut(s) 312, 531
BfoI RGCGCY 1 cut(s) 249
BfuAI ACCTGC 1 cut(s) 328
BisI GCNGC 4 cut(s) 61, 70, 576, 618
BlsI GCNGC 4 cut(s) 62, 71, 577, 619
Bme1390I CCNGG 1 cut(s) 541
BmeT110I CYCGRG 1 cut(s) 191
BmrFI CCNGG 1 cut(s) 541
BmrI ACTGGG 1 cut(s) 558
BmuI ACTGGG 1 cut(s) 558
BoxI GACNNNNGTC 1 cut(s) 470
BpiI GAAGAC 1 cut(s) 225
BpmI CTGGAG 2 cut(s) 22, 120
Bpu10I CCTNAGC 1 cut(s) 372
Bpu14I TTCGAA 1 cut(s) 623
BpuEI CTTGAG 2 cut(s) 168, 674
BpuMI CCSGG 1 cut(s) 541
BsaJI CCNNGG 1 cut(s) 498
Bse1I ACTGG 3 cut(s) 156, 553, 689
Bse3DI GCAATG 1 cut(s) 78
BseDI CCNNGG 1 cut(s) 498
BseGI GGATG 2 cut(s) 277, 438
BseMI GCAATG 1 cut(s) 78
BseMII CTCAG 1 cut(s) 275
BseNI ACTGG 3 cut(s) 156, 553, 689
BseXI GCAGC 4 cut(s) 72, 81, 562, 604
Bsh1236I CGCG 1 cut(s) 477
BshFI GGCC 3 cut(s) 231, 395, 591
BsiHKCI CYCGRG 1 cut(s) 191
BsiSI CCGG 1 cut(s) 540
BsmAI GTCTC 1 cut(s) 675
BsnI GGCC 3 cut(s) 231, 395, 591
BsoBI CYCGRG 1 cut(s) 191
Bsp119I TTCGAA 1 cut(s) 623
Bsp143I GATC 2 cut(s) 481, 636
BspACI CCGC 4 cut(s) 20, 147, 443, 475
BspANI GGCC 3 cut(s) 231, 395, 591
BspCNI CTCAG 1 cut(s) 274
BspFNI CGCG 1 cut(s) 477
BspMI ACCTGC 1 cut(s) 328
BspT104I TTCGAA 1 cut(s) 623
BsrDI GCAATG 1 cut(s) 78
BsrI ACTGG 3 cut(s) 156, 553, 689
BssECI CCNNGG 1 cut(s) 498
BssMI GATC 2 cut(s) 481, 636
BssSI CACGAG 1 cut(s) 204
BssT1I CCWWGG 1 cut(s) 498
Bst2BI CACGAG 1 cut(s) 204
Bst4CI ACNGT 1 cut(s) 598
Bst6I CTCTTC 1 cut(s) 668
BstAPI GCANNNNNTGC 1 cut(s) 584
BstBI TTCGAA 1 cut(s) 623
BstC8I GCNNGC 4 cut(s) 149, 153, 335, 589
BstDEI CTNAG 4 cut(s) 50, 261, 372, 386
BstF5I GGATG 2 cut(s) 277, 438
BstFNI CGCG 1 cut(s) 477
BstH2I RGCGCY 1 cut(s) 249
BstHHI GCGC 1 cut(s) 248
BstKTI GATC 2 cut(s) 484, 639
BstMAI GTCTC 1 cut(s) 675
BstMBI GATC 2 cut(s) 481, 636
BstMWI GCNNNNNNNGC 7 cut(s) 60, 66, 69, 161, 401, 572, 584
BstNSI RCATGY 1 cut(s) 226
BstPAI GACNNNNGTC 1 cut(s) 470
BstSCI CCNGG 1 cut(s) 539
BstUI CGCG 1 cut(s) 477
BstV1I GCAGC 4 cut(s) 72, 81, 562, 604
BstV2I GAAGAC 1 cut(s) 225
BstXI CCANNNNNNTGG 1 cut(s) 367
BsuRI GGCC 3 cut(s) 231, 395, 591
BtsCI GGATG 2 cut(s) 277, 438
BtsI GCAGTG 1 cut(s) 126
BtsIMutI CAGTG 3 cut(s) 126, 514, 639
BveI ACCTGC 1 cut(s) 328
Cac8I GCNNGC 4 cut(s) 149, 153, 335, 589
CfoI GCGC 1 cut(s) 248
Csp6I GTAC 2 cut(s) 291, 323
CviAII CATG 4 cut(s) 223, 227, 470, 568
CviQI GTAC 2 cut(s) 291, 323
DdeI CTNAG 4 cut(s) 50, 261, 372, 386
DpnI GATC 2 cut(s) 483, 638
DpnII GATC 2 cut(s) 481, 636
EaeI YGGCCR 1 cut(s) 393
Eam1104I CTCTTC 1 cut(s) 668
EarI CTCTTC 1 cut(s) 668
Eco130I CCWWGG 1 cut(s) 498
Eco32I GATATC 1 cut(s) 517
Eco88I CYCGRG 1 cut(s) 191
EcoRV GATATC 1 cut(s) 517
EcoT14I CCWWGG 1 cut(s) 498
EcoT22I ATGCAT 1 cut(s) 228
ErhI CCWWGG 1 cut(s) 498
FaeI CATG 4 cut(s) 226, 230, 473, 571
FatI CATG 4 cut(s) 222, 226, 469, 567
FauI CCCGC 2 cut(s) 140, 450
Fnu4HI GCNGC 4 cut(s) 61, 70, 576, 618
FokI GGATG 2 cut(s) 284, 425
Fsp4HI GCNGC 4 cut(s) 61, 70, 576, 618
FspBI CTAG 2 cut(s) 312, 531
GlaI GCGC 1 cut(s) 247
GluI GCNGC 4 cut(s) 61, 70, 576, 618
GsuI CTGGAG 2 cut(s) 22, 120
HaeII RGCGCY 1 cut(s) 249
HaeIII GGCC 3 cut(s) 231, 395, 591
HapII CCGG 1 cut(s) 540
HhaI GCGC 1 cut(s) 248
Hin1II CATG 4 cut(s) 226, 230, 473, 571
Hin6I GCGC 1 cut(s) 246
HinP1I GCGC 1 cut(s) 246
HpaII CCGG 1 cut(s) 540
HphI GGTGA 1 cut(s) 140
Hpy166II GTNNAC 1 cut(s) 131
Hpy188I TCNGA 3 cut(s) 264, 465, 486
Hpy188III TCNNGA 3 cut(s) 39, 185, 204
Hpy8I GTNNAC 1 cut(s) 131
HpyAV CCTTC 1 cut(s) 36
HpyCH4III ACNGT 1 cut(s) 598
HpyCH4V TGCA 5 cut(s) 164, 226, 318, 578, 612
HpyF10VI GCNNNNNNNGC 7 cut(s) 60, 66, 69, 161, 401, 572, 584
HpyF3I CTNAG 4 cut(s) 50, 261, 372, 386
Hsp92II CATG 4 cut(s) 226, 230, 473, 571
HspAI GCGC 1 cut(s) 246
Kzo9I GATC 2 cut(s) 481, 636
LmnI GCTCC 1 cut(s) 416
Lsp1109I GCAGC 4 cut(s) 72, 81, 562, 604
MaeI CTAG 2 cut(s) 312, 531
MalI GATC 2 cut(s) 483, 638
MboI GATC 2 cut(s) 481, 636
MboII GAAGA 2 cut(s) 230, 685
MfeI CAATTG 1 cut(s) 90
MlsI TGGCCA 1 cut(s) 395
MluCI AATT 1 cut(s) 90
MluNI TGGCCA 1 cut(s) 395
MnlI CCTC 4 cut(s) 144, 250, 471, 688
Mox20I TGGCCA 1 cut(s) 395
Mph1103I ATGCAT 1 cut(s) 228
MscI TGGCCA 1 cut(s) 395
MseI TTAA 1 cut(s) 102
Msp20I TGGCCA 1 cut(s) 395
MspA1I CMGCKG 1 cut(s) 575
MspI CCGG 1 cut(s) 540
MspR9I CCNGG 1 cut(s) 541
MunI CAATTG 1 cut(s) 90
MvnI CGCG 1 cut(s) 477
MwoI GCNNNNNNNGC 7 cut(s) 60, 66, 69, 161, 401, 572, 584
NciI CCSGG 1 cut(s) 541
NdeII GATC 2 cut(s) 481, 636
NlaIII CATG 4 cut(s) 226, 230, 473, 571
NsiI ATGCAT 1 cut(s) 228
NspI RCATGY 1 cut(s) 226
NspV TTCGAA 1 cut(s) 623
PaqCI CACCTGC 1 cut(s) 328
PkrI GCNGC 4 cut(s) 62, 71, 577, 619
PshAI GACNNNNGTC 1 cut(s) 470
PvuII CAGCTG 1 cut(s) 575
RsaI GTAC 2 cut(s) 292, 324
RsaNI GTAC 2 cut(s) 291, 323
SaqAI TTAA 1 cut(s) 102
SatI GCNGC 4 cut(s) 61, 70, 576, 618
Sau3AI GATC 2 cut(s) 481, 636
ScrFI CCNGG 1 cut(s) 541
SetI ASST 9 cut(s) 47, 102, 136, 324, 342, 463, 532, 577, 619
SfuI TTCGAA 1 cut(s) 623
SmlI CTYRAG 2 cut(s) 183, 653
SmoI CTYRAG 2 cut(s) 183, 653
Sse9I AATT 1 cut(s) 90
SsiI CCGC 4 cut(s) 20, 147, 443, 475
SspMI CTAG 2 cut(s) 312, 531
StyD4I CCNGG 1 cut(s) 539
StyI CCWWGG 1 cut(s) 498
TaaI ACNGT 1 cut(s) 598
TaqI TCGA 2 cut(s) 519, 623
TasI AATT 1 cut(s) 90
Tru1I TTAA 1 cut(s) 102
Tru9I TTAA 1 cut(s) 102
TscAI CASTG 3 cut(s) 126, 514, 646
TseI GCWGC 4 cut(s) 60, 69, 575, 617
TspRI CASTG 3 cut(s) 126, 514, 646
XceI RCATGY 1 cut(s) 226
XspI CTAG 2 cut(s) 312, 531
Zsp2I ATGCAT 1 cut(s) 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.