Rroxscaffold_4G00296780

Cyclic pyranopterin monophosphate synthase accessory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0012432
Physical Location & Seq
Forward (+)
16823453 .. 16825351
1899 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00296780.1

Sequence Viewer

Length: 705 bp
ATGTTTCTTCGGCGAATGGCGGTTTCGTTTTCTCACTCGACAAGGTTTCTTAGTAGCAGTAGCAGTAACAGCAGCATTGATATTGCGAAAGCGATTGATGAGCTTAACAAGGAAATGGAATCCGTATTTGGTGAACCTCCAGCAGATAGTGGGCTTGCCAGTTCTGCAAGAAATGATGTTACGGCTCAAGAACCTCAGTTAGCATCTCGAGAAAAAGGGGAAGATACGCATGGCTTAACCCACATTGGCAGTACAGGGGAGGCTCAAATGGTGGATGTTTCTCTCAAACAAAGTACCAAGAGAACTGCAGTTTCTAGTGGCAAGGTACTTCTTGGCAAGCAGGTGTTTGATTTGGTCTTAGCCAACCAGATGGCCAAGGGAGATGTCCTTAGTGTGGCTAAGATCGCTGGAATAAATGGAGCAAAGCACACAAGCAGTCTCATCCCGCTATGCCACAACATACCACTGACCCATGTCCGCGTTGATCTGACTCTAAATCCCAAGGATTTCAGTGTGGATATCGAAGCGGAAGCAAGTTCGACCGGGAAAACTGGGGTCGAAATGGAAGCAATGACTGCTGTGAGTGTTGCTGGCCTAACAGTCTATGATATGTGCAAAGCTGCTTCGAAGTCTATACAGATCACTGATATAAGACTTGAGAGTAAAACTGGTGGGAAGAGTGGAGACTGGTCCAGGGAGGAGTAA

Protein Analysis

234

Amino Acids

24.84

Weight (kDa)

5.9

Isoelectric Point (pI)

37.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MoaC PF01967 90 - 225 2.8e-54 MoaC family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012432)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01290 AT1G01290
fragaria_vesca FvH4_7g16680
malus_domestica MD07G1150800.v1.1
prunus_persica Prupe.2G189500_v2.0.a1 Prupe.2G189500_v2.0.a1 Prupe.2G189500_v2.0.a1
pyrus_communis pycom07g14910
rosa_chinensis RchiOBHm_Chr1g0359831
rosa_laevigata RLG00000027850
rosa_multiflora Rmu_sc0003191.1_g000005 Rmu_ssc0000371.1_g000002
rosa_roxburghii Rroxscaffold_4G00296780
rosa_rugosa Rorug01G0272900
rosa_samantha Rh1AG287000 Rh1BG252800 Rh1CG270000 Rh1DG281800
rosa_wichuraiana Rw1G025490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 331
Acc36I ACCTGC 1 cut(s) 331
AccII CGCG 1 cut(s) 480
AciI CCGC 4 cut(s) 20, 446, 478, 527
AcoI YGGCCR 1 cut(s) 372
AfaI GTAC 3 cut(s) 253, 295, 327
AfiI CCNNNNNNNGG 1 cut(s) 394
AjnI CCWGG 1 cut(s) 692
AjuI GAANNNNNNNTTGG 2 cut(s) 111, 143
AluBI AGCT 2 cut(s) 103, 620
AluI AGCT 2 cut(s) 103, 620
Alw26I GTCTC 2 cut(s) 443, 678
Ama87I CYCGRG 1 cut(s) 207
AoxI GGCC 2 cut(s) 372, 592
ApeKI GCWGC 2 cut(s) 72, 620
AspS9I GGNCC 1 cut(s) 690
AsuC2I CCSGG 1 cut(s) 544
AsuHPI GGTGA 1 cut(s) 143
AsuII TTCGAA 1 cut(s) 626
AvaI CYCGRG 1 cut(s) 207
AvaII GGWCC 1 cut(s) 690
BalI TGGCCA 1 cut(s) 374
BbvI GCAGC 2 cut(s) 84, 607
BccI CCATC 1 cut(s) 364
BceAI ACGGC 1 cut(s) 198
BciT130I CCWGG 1 cut(s) 694
BcnI CCSGG 1 cut(s) 544
BcoDI GTCTC 2 cut(s) 443, 678
BfaI CTAG 1 cut(s) 315
BfmI CTRYAG 1 cut(s) 306
BfuAI ACCTGC 1 cut(s) 331
BisI GCNGC 2 cut(s) 73, 621
BlsI GCNGC 2 cut(s) 74, 622
Bme1390I CCNGG 2 cut(s) 544, 694
Bme18I GGWCC 1 cut(s) 690
BmeT110I CYCGRG 1 cut(s) 207
BmgT120I GGNCC 1 cut(s) 690
BmrFI CCNGG 2 cut(s) 544, 694
BmrI ACTGGG 1 cut(s) 561
BmsI GCATC 1 cut(s) 212
BmuI ACTGGG 1 cut(s) 561
BoxI GACNNNNGTC 1 cut(s) 473
BpmI CTGGAG 1 cut(s) 123
Bpu14I TTCGAA 1 cut(s) 626
BpuEI CTTGAG 2 cut(s) 171, 677
BpuMI CCSGG 1 cut(s) 544
BsaJI CCNNGG 3 cut(s) 375, 501, 693
Bsc4I CCNNNNNNNGG 1 cut(s) 394
Bse1I ACTGG 4 cut(s) 159, 556, 673, 692
Bse3DI GCAATG 1 cut(s) 576
BseBI CCWGG 1 cut(s) 694
BseDI CCNNGG 3 cut(s) 375, 501, 693
BseGI GGATG 2 cut(s) 280, 441
BseLI CCNNNNNNNGG 1 cut(s) 394
BseMI GCAATG 1 cut(s) 576
BseMII CTCAG 1 cut(s) 209
BseNI ACTGG 4 cut(s) 159, 556, 673, 692
BseXI GCAGC 2 cut(s) 84, 607
Bsh1236I CGCG 1 cut(s) 480
Bsh1285I CGRYCG 1 cut(s) 543
BshFI GGCC 2 cut(s) 374, 594
BsiEI CGRYCG 1 cut(s) 543
BsiHKCI CYCGRG 1 cut(s) 207
BsiSI CCGG 1 cut(s) 543
BslI CCNNNNNNNGG 1 cut(s) 394
BsmAI GTCTC 2 cut(s) 443, 678
BsnI GGCC 2 cut(s) 374, 594
BsoBI CYCGRG 1 cut(s) 207
Bsp119I TTCGAA 1 cut(s) 626
Bsp143I GATC 3 cut(s) 402, 484, 639
BspACI CCGC 4 cut(s) 20, 446, 478, 527
BspANI GGCC 2 cut(s) 374, 594
BspCNI CTCAG 1 cut(s) 208
BspFNI CGCG 1 cut(s) 480
BspMAI CTGCAG 1 cut(s) 310
BspMI ACCTGC 1 cut(s) 331
BspT104I TTCGAA 1 cut(s) 626
BsrDI GCAATG 1 cut(s) 576
BsrI ACTGG 4 cut(s) 159, 556, 673, 692
BssECI CCNNGG 3 cut(s) 375, 501, 693
BssMI GATC 3 cut(s) 402, 484, 639
BssT1I CCWWGG 2 cut(s) 375, 501
Bst2UI CCWGG 1 cut(s) 694
Bst4CI ACNGT 1 cut(s) 601
Bst6I CTCTTC 1 cut(s) 671
BstAPI GCANNNNNTGC 1 cut(s) 575
BstBI TTCGAA 1 cut(s) 626
BstC8I GCNNGC 3 cut(s) 156, 338, 592
BstDEI CTNAG 5 cut(s) 50, 195, 358, 389, 399
BstF5I GGATG 2 cut(s) 280, 441
BstFNI CGCG 1 cut(s) 480
BstKTI GATC 3 cut(s) 405, 487, 642
BstMAI GTCTC 2 cut(s) 443, 678
BstMBI GATC 3 cut(s) 402, 484, 639
BstMCI CGRYCG 1 cut(s) 543
BstMWI GCNNNNNNNGC 4 cut(s) 69, 164, 404, 575
BstNI CCWGG 1 cut(s) 694
BstPAI GACNNNNGTC 1 cut(s) 473
BstSCI CCNGG 2 cut(s) 542, 692
BstSFI CTRYAG 1 cut(s) 306
BstUI CGCG 1 cut(s) 480
BstV1I GCAGC 2 cut(s) 84, 607
BstXI CCANNNNNNTGG 1 cut(s) 370
BsuRI GGCC 2 cut(s) 374, 594
BtsCI GGATG 2 cut(s) 280, 441
BtsIMutI CAGTG 3 cut(s) 464, 517, 642
BveI ACCTGC 1 cut(s) 331
Cac8I GCNNGC 3 cut(s) 156, 338, 592
Cfr13I GGNCC 1 cut(s) 690
Csp6I GTAC 3 cut(s) 252, 294, 326
CviAII CATG 2 cut(s) 230, 473
CviQI GTAC 3 cut(s) 252, 294, 326
DdeI CTNAG 5 cut(s) 50, 195, 358, 389, 399
DpnI GATC 3 cut(s) 404, 486, 641
DpnII GATC 3 cut(s) 402, 484, 639
EaeI YGGCCR 1 cut(s) 372
Eam1104I CTCTTC 1 cut(s) 671
EarI CTCTTC 1 cut(s) 671
Eco130I CCWWGG 2 cut(s) 375, 501
Eco32I GATATC 1 cut(s) 520
Eco47I GGWCC 1 cut(s) 690
Eco88I CYCGRG 1 cut(s) 207
EcoRII CCWGG 1 cut(s) 692
EcoRV GATATC 1 cut(s) 520
EcoT14I CCWWGG 2 cut(s) 375, 501
ErhI CCWWGG 2 cut(s) 375, 501
FaeI CATG 2 cut(s) 233, 476
FaiI YATR 8 cut(s) 231, 451, 461, 474, 606, 611, 635, 650
FatI CATG 2 cut(s) 229, 472
FauI CCCGC 1 cut(s) 453
Fnu4HI GCNGC 2 cut(s) 73, 621
FokI GGATG 2 cut(s) 287, 428
Fsp4HI GCNGC 2 cut(s) 73, 621
FspBI CTAG 1 cut(s) 315
GluI GCNGC 2 cut(s) 73, 621
GsuI CTGGAG 1 cut(s) 123
HaeIII GGCC 2 cut(s) 374, 594
HapII CCGG 1 cut(s) 543
Hin1II CATG 2 cut(s) 233, 476
HinfI GANTC 2 cut(s) 119, 490
HpaII CCGG 1 cut(s) 543
HphI GGTGA 1 cut(s) 143
Hpy166II GTNNAC 1 cut(s) 134
Hpy188I TCNGA 1 cut(s) 489
Hpy188III TCNNGA 3 cut(s) 188, 207, 209
Hpy8I GTNNAC 1 cut(s) 134
HpyCH4III ACNGT 1 cut(s) 601
HpyCH4V TGCA 3 cut(s) 167, 308, 615
HpyF10VI GCNNNNNNNGC 4 cut(s) 69, 164, 404, 575
HpyF3I CTNAG 5 cut(s) 50, 195, 358, 389, 399
Hsp92II CATG 2 cut(s) 233, 476
Kzo9I GATC 3 cut(s) 402, 484, 639
LmnI GCTCC 1 cut(s) 419
Lsp1109I GCAGC 2 cut(s) 84, 607
LweI GCATC 1 cut(s) 212
MaeI CTAG 1 cut(s) 315
MaeIII GTNAC 2 cut(s) 65, 178
MalI GATC 3 cut(s) 404, 486, 641
MboI GATC 3 cut(s) 402, 484, 639
MboII GAAGA 2 cut(s) 233, 688
MlsI TGGCCA 1 cut(s) 374
MluNI TGGCCA 1 cut(s) 374
MlyI GAGTC 1 cut(s) 484
MnlI CCTC 4 cut(s) 147, 204, 253, 691
Mox20I TGGCCA 1 cut(s) 374
MscI TGGCCA 1 cut(s) 374
MseI TTAA 2 cut(s) 105, 236
Msp20I TGGCCA 1 cut(s) 374
MspI CCGG 1 cut(s) 543
MspR9I CCNGG 2 cut(s) 544, 694
MvaI CCWGG 1 cut(s) 694
MvnI CGCG 1 cut(s) 480
MwoI GCNNNNNNNGC 4 cut(s) 69, 164, 404, 575
NciI CCSGG 1 cut(s) 544
NdeII GATC 3 cut(s) 402, 484, 639
NlaIII CATG 2 cut(s) 233, 476
NspV TTCGAA 1 cut(s) 626
PaeR7I CTCGAG 1 cut(s) 207
PaqCI CACCTGC 1 cut(s) 331
PfeI GAWTC 1 cut(s) 119
PkrI GCNGC 2 cut(s) 74, 622
PleI GAGTC 1 cut(s) 484
PpsI GAGTC 1 cut(s) 484
PshAI GACNNNNGTC 1 cut(s) 473
Psp6I CCWGG 1 cut(s) 692
PspGI CCWGG 1 cut(s) 692
PspPI GGNCC 1 cut(s) 690
PstI CTGCAG 1 cut(s) 310
RsaI GTAC 3 cut(s) 253, 295, 327
RsaNI GTAC 3 cut(s) 252, 294, 326
SaqAI TTAA 2 cut(s) 105, 236
SatI GCNGC 2 cut(s) 73, 621
Sau3AI GATC 3 cut(s) 402, 484, 639
Sau96I GGNCC 1 cut(s) 690
SchI GAGTC 1 cut(s) 484
ScrFI CCNGG 2 cut(s) 544, 694
SetI ASST 7 cut(s) 47, 105, 139, 196, 327, 345, 622
SfaNI GCATC 1 cut(s) 212
SfcI CTRYAG 1 cut(s) 306
Sfr274I CTCGAG 1 cut(s) 207
SfuI TTCGAA 1 cut(s) 626
SinI GGWCC 1 cut(s) 690
SlaI CTCGAG 1 cut(s) 207
SmlI CTYRAG 3 cut(s) 186, 207, 656
SmoI CTYRAG 3 cut(s) 186, 207, 656
SsiI CCGC 4 cut(s) 20, 446, 478, 527
SspMI CTAG 1 cut(s) 315
StyD4I CCNGG 2 cut(s) 542, 692
StyI CCWWGG 2 cut(s) 375, 501
TaaI ACNGT 1 cut(s) 601
TaqI TCGA 6 cut(s) 38, 208, 522, 539, 558, 626
TatI WGTACW 1 cut(s) 251
TfiI GAWTC 1 cut(s) 119
Tru1I TTAA 2 cut(s) 105, 236
Tru9I TTAA 2 cut(s) 105, 236
TscAI CASTG 3 cut(s) 471, 517, 649
TseI GCWGC 2 cut(s) 72, 620
TspGWI ACGGA 1 cut(s) 112
TspRI CASTG 3 cut(s) 471, 517, 649
VpaK11BI GGWCC 1 cut(s) 690
XhoI CTCGAG 1 cut(s) 207
XspI CTAG 1 cut(s) 315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.