MD07G1150800.v1.1

Cyclic pyranopterin monophosphate synthase accessory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0012432
Physical Location & Seq
Reverse (-)
21902306 .. 21904128
1823 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1150800.v1.1.491

Sequence Viewer

Length: 696 bp
ATGTTTCTTCGGAGGCTCGCGGTATCATCTTTCTGCTCGAGAAGGTTTTTTAGTACTAACAGCGGTAACAATGATCTTGCAAGAACTATTGCTGAGCTTAATAAGGAAATGGAATCGGTATTTGGTGAACATCCAGCAAGTGGACCCCCAAGTTTTCCAAATAACGATTCTATGGCTCAAGAACCCCAATTAGCACCTCAAGAAGAGGGCGAAAACGAACTTGTCCTAACTCACATTGGCAGTACAGGGGAAGCGCAAATGGTGGATGTTTCTCCCAAACAAAGCAGTAAGAGAACTGCCATTTCTAGCGGCAAGGTAATTCTTGGAAAGCAGGTGTTTGATTTGGTTGTAGCTAACCAGATGGCCAAGGGTGACGTCCTTAGCGTGGCGAAAATTGCTGGAATAAGTGGAGCAAAGCACACAAGTAATCTCATCCCGCTATGCCACAACATACCACTGACCCATGTTAGCATTAATCTGTCACTAAATCCGCAGGATTTCAGCGTGGACATTGAAGCGGAAGCCAGTTCAACAGGGAAAACAGGGGTTGAAATGGAAGCAATGACAGCAGTGAGTGTTGCTGGGCTAACAGTGTATGATATGTGCAAGGCTGCTTCGAAATCTATACAGATCACGGATATTCGACTCGAGAGTAAAACTGGTGGGAAGAGTGGGGACTGGTCCAGGGAGGAGTAA

Protein Analysis

232

Amino Acids

24.66

Weight (kDa)

5.44

Isoelectric Point (pI)

45.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MoaC PF01967 87 - 222 5.2e-54 MoaC family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012432)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01290 AT1G01290
fragaria_vesca FvH4_7g16680
malus_domestica MD07G1150800.v1.1
prunus_persica Prupe.2G189500_v2.0.a1 Prupe.2G189500_v2.0.a1 Prupe.2G189500_v2.0.a1
pyrus_communis pycom07g14910
rosa_chinensis RchiOBHm_Chr1g0359831
rosa_laevigata RLG00000027850
rosa_multiflora Rmu_sc0003191.1_g000005 Rmu_ssc0000371.1_g000002
rosa_roxburghii Rroxscaffold_4G00296780
rosa_rugosa Rorug01G0272900
rosa_samantha Rh1AG287000 Rh1BG252800 Rh1CG270000 Rh1DG281800
rosa_wichuraiana Rw1G025490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 322
AatII GACGTC 1 cut(s) 378
Acc36I ACCTGC 1 cut(s) 322
AccB7I CCANNNNNTGG 1 cut(s) 140
AccII CGCG 1 cut(s) 20
AciI CCGC 6 cut(s) 20, 63, 309, 437, 491, 518
AcoI YGGCCR 1 cut(s) 363
AcyI GRCGYC 1 cut(s) 375
AfaI GTAC 2 cut(s) 55, 244
AfiI CCNNNNNNNGG 2 cut(s) 140, 385
AgsI TTSAA 3 cut(s) 515, 531, 551
AjnI CCWGG 1 cut(s) 683
AjuI GAANNNNNNNTTGG 4 cut(s) 105, 137, 151, 183
AluBI AGCT 2 cut(s) 97, 353
AluI AGCT 2 cut(s) 97, 353
Ama87I CYCGRG 2 cut(s) 37, 647
AoxI GGCC 1 cut(s) 363
ApeKI GCWGC 1 cut(s) 611
AseI ATTAAT 1 cut(s) 474
AspLEI GCGC 1 cut(s) 256
AspS9I GGNCC 2 cut(s) 143, 681
AsuHPI GGTGA 2 cut(s) 137, 383
AsuII TTCGAA 1 cut(s) 617
AvaI CYCGRG 2 cut(s) 37, 647
AvaII GGWCC 2 cut(s) 143, 681
BalI TGGCCA 1 cut(s) 365
BbvI GCAGC 1 cut(s) 598
BccI CCATC 1 cut(s) 355
BciT130I CCWGG 1 cut(s) 685
BfaI CTAG 1 cut(s) 306
BfuAI ACCTGC 1 cut(s) 322
BisI GCNGC 2 cut(s) 310, 612
BlpI GCTNAGC 1 cut(s) 93
BlsI GCNGC 2 cut(s) 311, 613
BmcAI AGTACT 1 cut(s) 55
Bme1390I CCNGG 1 cut(s) 685
Bme18I GGWCC 2 cut(s) 143, 681
BmeT110I CYCGRG 2 cut(s) 37, 647
BmgT120I GGNCC 2 cut(s) 143, 681
BmiI GGNNCC 1 cut(s) 145
BmrFI CCNGG 1 cut(s) 685
Bpu10I CCTNAGC 1 cut(s) 380
Bpu1102I GCTNAGC 1 cut(s) 93
Bpu14I TTCGAA 1 cut(s) 617
BpuEI CTTGAG 2 cut(s) 162, 183
BsaHI GRCGYC 1 cut(s) 375
BsaJI CCNNGG 2 cut(s) 366, 684
Bsc4I CCNNNNNNNGG 2 cut(s) 140, 385
Bse1I ACTGG 3 cut(s) 525, 664, 683
Bse3DI GCAATG 1 cut(s) 567
BseBI CCWGG 1 cut(s) 685
BseDI CCNNGG 2 cut(s) 366, 684
BseGI GGATG 3 cut(s) 130, 271, 432
BseLI CCNNNNNNNGG 2 cut(s) 140, 385
BseMI GCAATG 1 cut(s) 567
BseMII CTCAG 1 cut(s) 84
BseNI ACTGG 3 cut(s) 525, 664, 683
BseXI GCAGC 1 cut(s) 598
BseYI CCCAGC 1 cut(s) 581
Bsh1236I CGCG 1 cut(s) 20
BshFI GGCC 1 cut(s) 365
BsiHKCI CYCGRG 2 cut(s) 37, 647
BslFI GGGAC 1 cut(s) 689
BslI CCNNNNNNNGG 2 cut(s) 140, 385
BsmFI GGGAC 1 cut(s) 689
BsnI GGCC 1 cut(s) 365
BsoBI CYCGRG 2 cut(s) 37, 647
Bsp119I TTCGAA 1 cut(s) 617
Bsp143I GATC 2 cut(s) 73, 630
Bsp1720I GCTNAGC 1 cut(s) 93
BspACI CCGC 6 cut(s) 20, 63, 309, 437, 491, 518
BspANI GGCC 1 cut(s) 365
BspCNI CTCAG 1 cut(s) 85
BspFNI CGCG 1 cut(s) 20
BspLI GGNNCC 1 cut(s) 145
BspMI ACCTGC 1 cut(s) 322
BspT104I TTCGAA 1 cut(s) 617
BsrDI GCAATG 1 cut(s) 567
BsrI ACTGG 3 cut(s) 525, 664, 683
BssECI CCNNGG 2 cut(s) 366, 684
BssMI GATC 2 cut(s) 73, 630
BssNI GRCGYC 1 cut(s) 375
BssT1I CCWWGG 1 cut(s) 366
Bst2UI CCWGG 1 cut(s) 685
Bst4CI ACNGT 1 cut(s) 592
Bst6I CTCTTC 2 cut(s) 198, 662
BstACI GRCGYC 1 cut(s) 375
BstBI TTCGAA 1 cut(s) 617
BstC8I GCNNGC 1 cut(s) 18
BstDEI CTNAG 2 cut(s) 93, 380
BstF5I GGATG 3 cut(s) 130, 271, 432
BstFNI CGCG 1 cut(s) 20
BstHHI GCGC 1 cut(s) 256
BstKTI GATC 2 cut(s) 76, 633
BstMBI GATC 2 cut(s) 73, 630
BstMWI GCNNNNNNNGC 2 cut(s) 395, 566
BstNI CCWGG 1 cut(s) 685
BstSCI CCNGG 1 cut(s) 683
BstUI CGCG 1 cut(s) 20
BstV1I GCAGC 1 cut(s) 598
BsuRI GGCC 1 cut(s) 365
BtsCI GGATG 3 cut(s) 130, 271, 432
BtsI GCAGTG 1 cut(s) 576
BtsIMutI CAGTG 3 cut(s) 455, 576, 597
BveI ACCTGC 1 cut(s) 322
Cac8I GCNNGC 1 cut(s) 18
CfoI GCGC 1 cut(s) 256
Cfr13I GGNCC 2 cut(s) 143, 681
Csp6I GTAC 2 cut(s) 54, 243
CviAII CATG 1 cut(s) 464
CviJI RGCY 8 cut(s) 16, 97, 176, 353, 365, 524, 586, 611
CviKI_1 RGCY 8 cut(s) 16, 97, 176, 353, 365, 524, 586, 611
CviQI GTAC 2 cut(s) 54, 243
DdeI CTNAG 2 cut(s) 93, 380
DpnI GATC 2 cut(s) 75, 632
DpnII GATC 2 cut(s) 73, 630
EaeI YGGCCR 1 cut(s) 363
Eam1104I CTCTTC 2 cut(s) 198, 662
EarI CTCTTC 2 cut(s) 198, 662
Eco130I CCWWGG 1 cut(s) 366
Eco47I GGWCC 2 cut(s) 143, 681
Eco88I CYCGRG 2 cut(s) 37, 647
EcoRII CCWGG 1 cut(s) 683
EcoT14I CCWWGG 1 cut(s) 366
ErhI CCWWGG 1 cut(s) 366
FaeI CATG 1 cut(s) 467
FaiI YATR 7 cut(s) 173, 442, 452, 465, 597, 602, 626
FaqI GGGAC 1 cut(s) 689
FatI CATG 1 cut(s) 463
FauI CCCGC 1 cut(s) 444
Fnu4HI GCNGC 2 cut(s) 310, 612
FokI GGATG 3 cut(s) 117, 278, 419
Fsp4HI GCNGC 2 cut(s) 310, 612
FspBI CTAG 1 cut(s) 306
GlaI GCGC 1 cut(s) 255
GluI GCNGC 2 cut(s) 310, 612
GsaI CCCAGC 1 cut(s) 585
HaeIII GGCC 1 cut(s) 365
HhaI GCGC 1 cut(s) 256
Hin1I GRCGYC 1 cut(s) 375
Hin1II CATG 1 cut(s) 467
Hin6I GCGC 1 cut(s) 254
HinP1I GCGC 1 cut(s) 254
HinfI GANTC 3 cut(s) 113, 167, 645
HphI GGTGA 2 cut(s) 137, 383
Hpy166II GTNNAC 3 cut(s) 128, 143, 508
Hpy188I TCNGA 1 cut(s) 12
Hpy188III TCNNGA 4 cut(s) 39, 179, 200, 649
Hpy8I GTNNAC 3 cut(s) 128, 143, 508
HpyAV CCTTC 1 cut(s) 36
HpyCH4III ACNGT 1 cut(s) 592
HpyCH4IV ACGT 1 cut(s) 375
HpyCH4V TGCA 2 cut(s) 80, 606
HpyF10VI GCNNNNNNNGC 2 cut(s) 395, 566
HpyF3I CTNAG 2 cut(s) 93, 380
HpySE526I ACGT 1 cut(s) 375
Hsp92I GRCGYC 1 cut(s) 375
Hsp92II CATG 1 cut(s) 467
HspAI GCGC 1 cut(s) 254
Kzo9I GATC 2 cut(s) 73, 630
LmnI GCTCC 1 cut(s) 410
Lsp1109I GCAGC 1 cut(s) 598
MaeI CTAG 1 cut(s) 306
MaeII ACGT 1 cut(s) 375
MaeIII GTNAC 3 cut(s) 65, 371, 480
MalI GATC 2 cut(s) 75, 632
MboI GATC 2 cut(s) 73, 630
MboII GAAGA 2 cut(s) 215, 679
MlsI TGGCCA 1 cut(s) 365
MluCI AATT 3 cut(s) 188, 318, 393
MluNI TGGCCA 1 cut(s) 365
MlyI GAGTC 1 cut(s) 639
MnlI CCTC 4 cut(s) 6, 199, 207, 682
Mox20I TGGCCA 1 cut(s) 365
MscI TGGCCA 1 cut(s) 365
MseI TTAA 2 cut(s) 99, 474
Msp20I TGGCCA 1 cut(s) 365
MspA1I CMGCKG 1 cut(s) 63
MspR9I CCNGG 1 cut(s) 685
MvaI CCWGG 1 cut(s) 685
MvnI CGCG 1 cut(s) 20
MwoI GCNNNNNNNGC 2 cut(s) 395, 566
NdeII GATC 2 cut(s) 73, 630
NlaIII CATG 1 cut(s) 467
NlaIV GGNNCC 1 cut(s) 145
NmuCI GTSAC 2 cut(s) 371, 480
NspV TTCGAA 1 cut(s) 617
PaeR7I CTCGAG 2 cut(s) 37, 647
PaqCI CACCTGC 1 cut(s) 322
PcsI WCGNNNNNNNCGW 1 cut(s) 381
PfeI GAWTC 2 cut(s) 113, 167
PflMI CCANNNNNTGG 1 cut(s) 140
PkrI GCNGC 2 cut(s) 311, 613
PleI GAGTC 1 cut(s) 639
PpsI GAGTC 1 cut(s) 639
PshBI ATTAAT 1 cut(s) 474
Psp6I CCWGG 1 cut(s) 683
PspFI CCCAGC 1 cut(s) 581
PspGI CCWGG 1 cut(s) 683
PspN4I GGNNCC 1 cut(s) 145
PspPI GGNCC 2 cut(s) 143, 681
RsaI GTAC 2 cut(s) 55, 244
RsaNI GTAC 2 cut(s) 54, 243
SaqAI TTAA 2 cut(s) 99, 474
SatI GCNGC 2 cut(s) 310, 612
Sau3AI GATC 2 cut(s) 73, 630
Sau96I GGNCC 2 cut(s) 143, 681
ScaI AGTACT 1 cut(s) 55
SchI GAGTC 1 cut(s) 639
ScrFI CCNGG 1 cut(s) 685
SetI ASST 7 cut(s) 47, 99, 199, 318, 336, 355, 378
Sfr274I CTCGAG 2 cut(s) 37, 647
SfuI TTCGAA 1 cut(s) 617
SinI GGWCC 2 cut(s) 143, 681
SlaI CTCGAG 2 cut(s) 37, 647
SmlI CTYRAG 4 cut(s) 37, 177, 198, 647
SmoI CTYRAG 4 cut(s) 37, 177, 198, 647
Sse9I AATT 3 cut(s) 188, 318, 393
SsiI CCGC 6 cut(s) 20, 63, 309, 437, 491, 518
SspMI CTAG 1 cut(s) 306
StyD4I CCNGG 1 cut(s) 683
StyI CCWWGG 1 cut(s) 366
TaaI ACNGT 1 cut(s) 592
TaiI ACGT 1 cut(s) 378
TaqI TCGA 4 cut(s) 38, 617, 643, 648
TasI AATT 3 cut(s) 188, 318, 393
TatI WGTACW 2 cut(s) 53, 242
TauI GCSGC 1 cut(s) 312
TfiI GAWTC 2 cut(s) 113, 167
Tru1I TTAA 2 cut(s) 99, 474
Tru9I TTAA 2 cut(s) 99, 474
TscAI CASTG 3 cut(s) 462, 576, 597
TseFI GTSAC 2 cut(s) 371, 480
TseI GCWGC 1 cut(s) 611
Tsp45I GTSAC 2 cut(s) 371, 480
TspGWI ACGGA 1 cut(s) 650
TspRI CASTG 3 cut(s) 462, 576, 597
Van91I CCANNNNNTGG 1 cut(s) 140
VpaK11BI GGWCC 2 cut(s) 143, 681
VspI ATTAAT 1 cut(s) 474
XhoI CTCGAG 2 cut(s) 37, 647
XspI CTAG 1 cut(s) 306
ZraI GACGTC 1 cut(s) 376
ZrmI AGTACT 1 cut(s) 55
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.