Rw1G025490

Cyclic pyranopterin monophosphate synthase accessory protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0012432
Physical Location & Seq
Reverse (-)
52165257 .. 52166468
1212 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G025490.1

Sequence Viewer

Length: 717 bp
ATGTTTCTTCGGCGAATGGCGGTTTCGTTTTCTCACTCGAGAAGGTTTCTTAGTAGCAGTAGCAGTAGCAGTAGCAGTAACAGCAGCATTGATATTGCGAAAGCGATTGATGAGCTGAACAAGGAAATGGAATCCGTATTTGGTGAACCTCCAGCAGATAGTGGGCTTGCCAGTTCTGCAAGAAATGATGTTACGGCTCAAGAACCTGAGTTAGCATCTCAAGAAAAAAGGGAAGATACGCATGACCTAACCCACATTGGCAGTACAGGGGAGGCTCAAATGGTGGATGTTTCTCCCAAACAAAGTACCAAGAGAACTGCCGTTTCTAGTGGCAAGGTACTTCTTGGCAAGCAGGTGTTTGATTTGGTCTTAGCCAACCAGATGGCCAAGGGAGATGTCCTTAGTGTGGCCAAGATTGCTGGAATAAATGGAGCAAAGTACACAAGCAGTCTCATCCCGCTATGCCACAACATACCACTGACCCATGTCCGCGTTGATCTGACTCTAAATCCCAAGGATTTCAGTGTGGACATCAAAGCAGAAGCAAGTTCAACCGGGAAAACTGGGGTCGAAATGGAAGCAATGACTGCTGTGAGTGTTGCTGGCCTAACAGTCTATGATATGTGCAAAGCTGCTTCGAAGTCTATACATATCACTGATTTAAGACTTGAGAGTAAAGCTGGTGGGAAGAGTGGAGACTGGTCCAGGGAGGAGTAA

Protein Analysis

238

Amino Acids

25.36

Weight (kDa)

6.09

Isoelectric Point (pI)

49.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MoaC PF01967 94 - 229 4.5e-53 MoaC family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012432)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01290 AT1G01290
fragaria_vesca FvH4_7g16680
malus_domestica MD07G1150800.v1.1
prunus_persica Prupe.2G189500_v2.0.a1 Prupe.2G189500_v2.0.a1 Prupe.2G189500_v2.0.a1
pyrus_communis pycom07g14910
rosa_chinensis RchiOBHm_Chr1g0359831
rosa_laevigata RLG00000027850
rosa_multiflora Rmu_sc0003191.1_g000005 Rmu_ssc0000371.1_g000002
rosa_roxburghii Rroxscaffold_4G00296780
rosa_rugosa Rorug01G0272900
rosa_samantha Rh1AG287000 Rh1BG252800 Rh1CG270000 Rh1DG281800
rosa_wichuraiana Rw1G025490

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 343
Acc36I ACCTGC 1 cut(s) 343
AccII CGCG 1 cut(s) 492
AciI CCGC 3 cut(s) 20, 458, 490
AcoI YGGCCR 2 cut(s) 384, 408
AfaI GTAC 4 cut(s) 265, 307, 339, 440
AfiI CCNNNNNNNGG 1 cut(s) 406
AgsI TTSAA 1 cut(s) 552
AjnI CCWGG 1 cut(s) 704
AjuI GAANNNNNNNTTGG 2 cut(s) 123, 155
AluBI AGCT 3 cut(s) 115, 632, 680
AluI AGCT 3 cut(s) 115, 632, 680
Alw26I GTCTC 2 cut(s) 455, 690
Ama87I CYCGRG 1 cut(s) 37
AoxI GGCC 3 cut(s) 384, 408, 604
ApeKI GCWGC 2 cut(s) 84, 632
AspS9I GGNCC 1 cut(s) 702
AsuC2I CCSGG 1 cut(s) 556
AsuHPI GGTGA 1 cut(s) 155
AsuII TTCGAA 1 cut(s) 638
AvaI CYCGRG 1 cut(s) 37
AvaII GGWCC 1 cut(s) 702
BalI TGGCCA 2 cut(s) 386, 410
BbvI GCAGC 2 cut(s) 96, 619
BccI CCATC 1 cut(s) 376
BceAI ACGGC 2 cut(s) 210, 305
BciT130I CCWGG 1 cut(s) 706
BcnI CCSGG 1 cut(s) 556
BcoDI GTCTC 2 cut(s) 455, 690
BfaI CTAG 1 cut(s) 327
BfuAI ACCTGC 1 cut(s) 343
BisI GCNGC 2 cut(s) 85, 633
BlsI GCNGC 2 cut(s) 86, 634
Bme1390I CCNGG 2 cut(s) 556, 706
Bme18I GGWCC 1 cut(s) 702
BmeT110I CYCGRG 1 cut(s) 37
BmgT120I GGNCC 1 cut(s) 702
BmrFI CCNGG 2 cut(s) 556, 706
BmrI ACTGGG 1 cut(s) 573
BmsI GCATC 1 cut(s) 224
BmuI ACTGGG 1 cut(s) 573
BoxI GACNNNNGTC 1 cut(s) 485
BpmI CTGGAG 1 cut(s) 135
Bpu14I TTCGAA 1 cut(s) 638
BpuEI CTTGAG 3 cut(s) 183, 204, 689
BpuMI CCSGG 1 cut(s) 556
BsaJI CCNNGG 3 cut(s) 387, 513, 705
Bsc4I CCNNNNNNNGG 1 cut(s) 406
Bse1I ACTGG 3 cut(s) 171, 568, 704
Bse3DI GCAATG 1 cut(s) 588
BseBI CCWGG 1 cut(s) 706
BseDI CCNNGG 3 cut(s) 387, 513, 705
BseGI GGATG 2 cut(s) 292, 453
BseLI CCNNNNNNNGG 1 cut(s) 406
BseMI GCAATG 1 cut(s) 588
BseMII CTCAG 1 cut(s) 198
BseNI ACTGG 3 cut(s) 171, 568, 704
BseXI GCAGC 2 cut(s) 96, 619
Bsh1236I CGCG 1 cut(s) 492
BshFI GGCC 3 cut(s) 386, 410, 606
BsiHKCI CYCGRG 1 cut(s) 37
BsiSI CCGG 1 cut(s) 555
BslI CCNNNNNNNGG 1 cut(s) 406
BsmAI GTCTC 2 cut(s) 455, 690
BsnI GGCC 3 cut(s) 386, 410, 606
BsoBI CYCGRG 1 cut(s) 37
Bsp119I TTCGAA 1 cut(s) 638
Bsp143I GATC 1 cut(s) 496
BspACI CCGC 3 cut(s) 20, 458, 490
BspANI GGCC 3 cut(s) 386, 410, 606
BspCNI CTCAG 1 cut(s) 199
BspFNI CGCG 1 cut(s) 492
BspMI ACCTGC 1 cut(s) 343
BspT104I TTCGAA 1 cut(s) 638
BsrDI GCAATG 1 cut(s) 588
BsrI ACTGG 3 cut(s) 171, 568, 704
BssECI CCNNGG 3 cut(s) 387, 513, 705
BssMI GATC 1 cut(s) 496
BssT1I CCWWGG 2 cut(s) 387, 513
Bst2UI CCWGG 1 cut(s) 706
Bst4CI ACNGT 1 cut(s) 613
Bst6I CTCTTC 1 cut(s) 683
BstAPI GCANNNNNTGC 1 cut(s) 587
BstBI TTCGAA 1 cut(s) 638
BstC8I GCNNGC 3 cut(s) 168, 350, 604
BstDEI CTNAG 4 cut(s) 50, 207, 370, 401
BstF5I GGATG 2 cut(s) 292, 453
BstFNI CGCG 1 cut(s) 492
BstKTI GATC 1 cut(s) 499
BstMAI GTCTC 2 cut(s) 455, 690
BstMBI GATC 1 cut(s) 496
BstMWI GCNNNNNNNGC 4 cut(s) 81, 176, 416, 587
BstNI CCWGG 1 cut(s) 706
BstPAI GACNNNNGTC 1 cut(s) 485
BstSCI CCNGG 2 cut(s) 554, 704
BstUI CGCG 1 cut(s) 492
BstV1I GCAGC 2 cut(s) 96, 619
BstXI CCANNNNNNTGG 1 cut(s) 382
BsuRI GGCC 3 cut(s) 386, 410, 606
BtsCI GGATG 2 cut(s) 292, 453
BtsIMutI CAGTG 3 cut(s) 476, 529, 654
BveI ACCTGC 1 cut(s) 343
Cac8I GCNNGC 3 cut(s) 168, 350, 604
Cfr13I GGNCC 1 cut(s) 702
Csp6I GTAC 4 cut(s) 264, 306, 338, 439
CviAII CATG 2 cut(s) 242, 485
CviQI GTAC 4 cut(s) 264, 306, 338, 439
DdeI CTNAG 4 cut(s) 50, 207, 370, 401
DpnI GATC 1 cut(s) 498
DpnII GATC 1 cut(s) 496
EaeI YGGCCR 2 cut(s) 384, 408
Eam1104I CTCTTC 1 cut(s) 683
EarI CTCTTC 1 cut(s) 683
Eco130I CCWWGG 2 cut(s) 387, 513
Eco47I GGWCC 1 cut(s) 702
Eco88I CYCGRG 1 cut(s) 37
EcoRII CCWGG 1 cut(s) 704
EcoT14I CCWWGG 2 cut(s) 387, 513
ErhI CCWWGG 2 cut(s) 387, 513
FaeI CATG 2 cut(s) 245, 488
FaiI YATR 8 cut(s) 243, 463, 473, 486, 618, 623, 647, 651
FatI CATG 2 cut(s) 241, 484
FauI CCCGC 1 cut(s) 465
Fnu4HI GCNGC 2 cut(s) 85, 633
FokI GGATG 2 cut(s) 299, 440
Fsp4HI GCNGC 2 cut(s) 85, 633
FspBI CTAG 1 cut(s) 327
GluI GCNGC 2 cut(s) 85, 633
GsuI CTGGAG 1 cut(s) 135
HaeIII GGCC 3 cut(s) 386, 410, 606
HapII CCGG 1 cut(s) 555
Hin1II CATG 2 cut(s) 245, 488
HinfI GANTC 2 cut(s) 131, 502
HpaII CCGG 1 cut(s) 555
HphI GGTGA 1 cut(s) 155
Hpy166II GTNNAC 3 cut(s) 146, 441, 529
Hpy188I TCNGA 1 cut(s) 501
Hpy188III TCNNGA 3 cut(s) 39, 200, 221
Hpy8I GTNNAC 3 cut(s) 146, 441, 529
HpyAV CCTTC 1 cut(s) 36
HpyCH4III ACNGT 1 cut(s) 613
HpyCH4V TGCA 2 cut(s) 179, 627
HpyF10VI GCNNNNNNNGC 4 cut(s) 81, 176, 416, 587
HpyF3I CTNAG 4 cut(s) 50, 207, 370, 401
Hsp92II CATG 2 cut(s) 245, 488
Kzo9I GATC 1 cut(s) 496
LmnI GCTCC 1 cut(s) 431
Lsp1109I GCAGC 2 cut(s) 96, 619
LweI GCATC 1 cut(s) 224
MaeI CTAG 1 cut(s) 327
MaeIII GTNAC 2 cut(s) 77, 190
MalI GATC 1 cut(s) 498
MboI GATC 1 cut(s) 496
MboII GAAGA 2 cut(s) 245, 700
MlsI TGGCCA 2 cut(s) 386, 410
MluNI TGGCCA 2 cut(s) 386, 410
MlyI GAGTC 1 cut(s) 496
MnlI CCTC 3 cut(s) 159, 265, 703
Mox20I TGGCCA 2 cut(s) 386, 410
MscI TGGCCA 2 cut(s) 386, 410
MseI TTAA 1 cut(s) 662
Msp20I TGGCCA 2 cut(s) 386, 410
MspI CCGG 1 cut(s) 555
MspR9I CCNGG 2 cut(s) 556, 706
MvaI CCWGG 1 cut(s) 706
MvnI CGCG 1 cut(s) 492
MwoI GCNNNNNNNGC 4 cut(s) 81, 176, 416, 587
NciI CCSGG 1 cut(s) 556
NdeII GATC 1 cut(s) 496
NlaIII CATG 2 cut(s) 245, 488
NspV TTCGAA 1 cut(s) 638
PaeR7I CTCGAG 1 cut(s) 37
PaqCI CACCTGC 1 cut(s) 343
PfeI GAWTC 1 cut(s) 131
PkrI GCNGC 2 cut(s) 86, 634
PleI GAGTC 1 cut(s) 496
PpsI GAGTC 1 cut(s) 496
PshAI GACNNNNGTC 1 cut(s) 485
Psp6I CCWGG 1 cut(s) 704
PspGI CCWGG 1 cut(s) 704
PspPI GGNCC 1 cut(s) 702
RsaI GTAC 4 cut(s) 265, 307, 339, 440
RsaNI GTAC 4 cut(s) 264, 306, 338, 439
SaqAI TTAA 1 cut(s) 662
SatI GCNGC 2 cut(s) 85, 633
Sau3AI GATC 1 cut(s) 496
Sau96I GGNCC 1 cut(s) 702
SchI GAGTC 1 cut(s) 496
ScrFI CCNGG 2 cut(s) 556, 706
SetI ASST 9 cut(s) 47, 117, 151, 208, 249, 339, 357, 634, 682
SfaNI GCATC 1 cut(s) 224
Sfr274I CTCGAG 1 cut(s) 37
SfuI TTCGAA 1 cut(s) 638
SinI GGWCC 1 cut(s) 702
SlaI CTCGAG 1 cut(s) 37
SmlI CTYRAG 4 cut(s) 37, 198, 219, 668
SmoI CTYRAG 4 cut(s) 37, 198, 219, 668
SsiI CCGC 3 cut(s) 20, 458, 490
SspMI CTAG 1 cut(s) 327
StyD4I CCNGG 2 cut(s) 554, 704
StyI CCWWGG 2 cut(s) 387, 513
TaaI ACNGT 1 cut(s) 613
TaqI TCGA 3 cut(s) 38, 570, 638
TatI WGTACW 2 cut(s) 263, 438
TfiI GAWTC 1 cut(s) 131
Tru1I TTAA 1 cut(s) 662
Tru9I TTAA 1 cut(s) 662
TscAI CASTG 3 cut(s) 483, 529, 661
TseI GCWGC 2 cut(s) 84, 632
TspGWI ACGGA 1 cut(s) 124
TspRI CASTG 3 cut(s) 483, 529, 661
VpaK11BI GGWCC 1 cut(s) 702
XhoI CTCGAG 1 cut(s) 37
XspI CTAG 1 cut(s) 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.