RLG00000027851

Belongs to the peptidase A1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0015794
Physical Location & Seq
Forward (+)
16054948 .. 16056113
1166 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027851

Sequence Viewer

Length: 1065 bp
ATGGTCCTCGACACCGGCTCCGACGTCGTTTGGCTCCAGTGCGCCCCATGCAAACGCTGCTACGCCCAAACCGACCCGGTCTTCGACCCGAGAAAATCCTCCACCTCCTCCCCCATCCCTTGCTCCTCCCCCCTCTGCCGCAAGCTCGACACCCCCGGCTGCAACTCCAGGAAGACCTGCCTCTACCAAGTCTCCTACGGCGACGGCTCCTTCACCGTCGGCGAGTTCTCCACCGAAACCCTAACCTTCCGCCGCTCCAAAGTCCCGCGCGTCGCCGTCGGCTGCGGCCACGACAACGAAGGCCTCTTCGTCGGCGCCGCCGGTCTGTTGGGTCTCGGCCGCGGGAAGCTATCGTTCCCCTCCCAGACCGGTCCCCGGTTCAAGTCCAAATTCTCCTACTGCCTCGTAGACCGGTCCGCCTCCTCCAAACCCTCCTCCGTCGTTTTCGGCGACTCGGCCATCTCCCGAACCGCCCGGTTCACTCCTCTCGTCTCCAACCCGAAGCTCGACACGTTCTACTACGTCGAACTCATCGGTATCAGCGTCGGCGGGACACGTGTTCCGGGAATCACGGCCTCGTTGTTCAAACTCGACCCGGCTGGAAACGGTGGGGTCATAATCGACTCGGGCACCTCCGTGACCCGGTTGACCCGATCCGCTTACACCTCCCTAAGGAATGCTTTCCGGGCCGGAACCCCGACCCTGAAGCGGGCGCCCGAGTTTTCTCTCTTCGATACTTGCTTTGATCTCTCCGGTAAGACGGAGGTGAAGGTTCCGACGGTGGTGCTGCATTTCCGAGGCGCCGACGTGTCGTTGCCGGCGACGAACTACTTGATTCCGGTGGATAGTAGCGGCACCTTCTGCTTTGCTTTTGCGGGTACGATGGGCGGGTTGTCCATAATTGGGAATATCCAGCAGCAAGGGTTTCGGGTGGTTTATGACTTGGCGGGTTCGCGGGTCGGGTTTGCTCCACATAAAAGATCAAAAAAGGAGAATACAAGAAATCTTTCTTGCATTTTAAGGTCTAGGAATATTGCGATGTTGTCTTGTTTAAAAGGTTTTTAG

Protein Analysis

355

Amino Acids

38.0

Weight (kDa)

9.59

Isoelectric Point (pI)

41.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TAXi_N PF14543 1 - 151 1.7e-50 Xylanase inhibitor N-terminal
Asp PF00026 62 - 324 1.9e-14 Eukaryotic aspartyl protease
TAXi_C PF14541 173 - 323 3.6e-35 Xylanase inhibitor C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015794)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01300 AT3G61820
fragaria_vesca FvH4_7g16670
malus_domestica MD01G1082200.v1.1 MD07G1150600.v1.1
prunus_persica Prupe.2G189400_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0359821
rosa_laevigata RLG00000027851
rosa_roxburghii Rroxscaffold_4G00296790
rosa_rugosa Rorug01G0272900
rosa_samantha Rh1AG286900 Rh1BG252700 Rh1CG269900 Rh1DG281700
rosa_wichuraiana Rw1G025480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 27
Acc36I ACCTGC 1 cut(s) 185
AccB1I GGYRCC 5 cut(s) 314, 629, 712, 800, 854
AccBSI CCGCTC 1 cut(s) 255
AccI GTMKAC 1 cut(s) 408
AccII CGCG 4 cut(s) 268, 270, 342, 955
AclWI GGATC 1 cut(s) 648
AcoI YGGCCR 3 cut(s) 286, 337, 456
AcsI RAATTY 1 cut(s) 389
AcuI CTGAAG 1 cut(s) 725
AcvI CACGTG 1 cut(s) 557
AcyI GRCGYC 4 cut(s) 24, 315, 713, 801
AfaI GTAC 1 cut(s) 880
AfiI CCNNNNNNNGG 6 cut(s) 375, 642, 672, 708, 709, 903
AflIII ACRYGT 4 cut(s) 510, 554, 556, 807
AgeI ACCGGT 2 cut(s) 368, 411
AgsI TTSAA 2 cut(s) 382, 586
AjiI CACGTC 1 cut(s) 808
AjnI CCWGG 1 cut(s) 167
AleI CACNNNNGTG 1 cut(s) 635
AluBI AGCT 3 cut(s) 145, 349, 505
AluI AGCT 3 cut(s) 145, 349, 505
Alw26I GTCTC 3 cut(s) 196, 338, 496
AlwI GGATC 1 cut(s) 648
Ama87I CYCGRG 3 cut(s) 88, 625, 716
AoxI GGCC 6 cut(s) 286, 301, 337, 456, 573, 687
ApeKI GCWGC 5 cut(s) 57, 159, 282, 787, 916
ApoI RAATTY 1 cut(s) 389
ArsI GACNNNNNNTTYG 2 cut(s) 65, 97
AsiGI ACCGGT 2 cut(s) 368, 411
Asp700I GAANNNNTTC 2 cut(s) 680, 1006
AspLEI GCGC 5 cut(s) 44, 270, 317, 715, 803
AspS9I GGNCC 4 cut(s) 4, 371, 414, 687
AsuC2I CCSGG 8 cut(s) 77, 156, 376, 475, 564, 596, 643, 686
AsuHPI GGTGA 2 cut(s) 205, 778
AvaI CYCGRG 3 cut(s) 88, 625, 716
AvaII GGWCC 3 cut(s) 4, 371, 414
AxyI CCTNAGG 1 cut(s) 671
BaeGI GKGCMC 1 cut(s) 632
BanI GGYRCC 5 cut(s) 314, 629, 712, 800, 854
BbrPI CACGTG 1 cut(s) 557
BbsI GAAGAC 2 cut(s) 73, 179
BbvI GCAGC 5 cut(s) 44, 146, 269, 774, 928
BccI CCATC 3 cut(s) 122, 467, 877
BceAI ACGGC 4 cut(s) 214, 220, 260, 588
BcgI CGANNNNNNTGC 4 cut(s) 766, 800, 908, 942
BciT130I CCWGG 1 cut(s) 169
BcnI CCSGG 8 cut(s) 77, 156, 376, 475, 564, 596, 643, 686
BcoDI GTCTC 3 cut(s) 196, 338, 496
BfaI CTAG 1 cut(s) 1026
BfoI RGCGCY 3 cut(s) 318, 716, 804
BfuAI ACCTGC 1 cut(s) 185
Bme1390I CCNGG 9 cut(s) 77, 156, 169, 376, 475, 564, 596, 643, 686
Bme18I GGWCC 3 cut(s) 4, 371, 414
BmeT110I CYCGRG 3 cut(s) 88, 625, 716
BmgBI CACGTC 1 cut(s) 808
BmgT120I GGNCC 4 cut(s) 4, 371, 414, 687
BmrFI CCNGG 9 cut(s) 77, 156, 169, 376, 475, 564, 596, 643, 686
BpiI GAAGAC 2 cut(s) 73, 179
BpmI CTGGAG 2 cut(s) 20, 151
BpuMI CCSGG 8 cut(s) 77, 156, 376, 475, 564, 596, 643, 686
BsaAI YACGTR 1 cut(s) 557
BsaHI GRCGYC 4 cut(s) 24, 315, 713, 801
BsaI GGTCTC 1 cut(s) 338
BsaJI CCNNGG 4 cut(s) 154, 340, 374, 796
BsaWI WCCGGW 4 cut(s) 368, 411, 752, 838
BsaXI ACNNNNNCTCC 3 cut(s) 32, 176, 206
Bsc4I CCNNNNNNNGG 6 cut(s) 375, 642, 672, 708, 709, 903
Bse118I RCCGGY 5 cut(s) 14, 320, 368, 411, 817
Bse1I ACTGG 1 cut(s) 37
Bse21I CCTNAGG 1 cut(s) 671
BseBI CCWGG 1 cut(s) 169
BseDI CCNNGG 4 cut(s) 154, 340, 374, 796
BseGI GGATG 1 cut(s) 114
BseLI CCNNNNNNNGG 6 cut(s) 375, 642, 672, 708, 709, 903
BseNI ACTGG 1 cut(s) 37
BseRI GAGGAG 5 cut(s) 97, 115, 412, 424, 474
BseSI GKGCMC 1 cut(s) 632
BseX3I CGGCCG 1 cut(s) 337
BseXI GCAGC 5 cut(s) 44, 146, 269, 774, 928
Bsh1236I CGCG 4 cut(s) 268, 270, 342, 955
Bsh1285I CGRYCG 1 cut(s) 340
BshFI GGCC 6 cut(s) 288, 303, 339, 458, 575, 689
BshNI GGYRCC 5 cut(s) 314, 629, 712, 800, 854
BshTI ACCGGT 2 cut(s) 368, 411
BsiEI CGRYCG 1 cut(s) 340
BsiHKCI CYCGRG 3 cut(s) 88, 625, 716
BslFI GGGAC 3 cut(s) 248, 357, 565
BslI CCNNNNNNNGG 6 cut(s) 375, 642, 672, 708, 709, 903
BsmAI GTCTC 3 cut(s) 196, 338, 496
BsmBI CGTCTC 1 cut(s) 496
BsmFI GGGAC 3 cut(s) 248, 357, 565
BsmI GAATGC 1 cut(s) 682
BsnI GGCC 6 cut(s) 288, 303, 339, 458, 575, 689
Bso31I GGTCTC 1 cut(s) 338
BsoBI CYCGRG 3 cut(s) 88, 625, 716
Bsp1286I GDGCHC 1 cut(s) 632
Bsp143I GATC 3 cut(s) 653, 745, 980
BspANI GGCC 6 cut(s) 288, 303, 339, 458, 575, 689
BspFNI CGCG 4 cut(s) 268, 270, 342, 955
BspMI ACCTGC 1 cut(s) 185
BspPI GGATC 1 cut(s) 648
BspT107I GGYRCC 5 cut(s) 314, 629, 712, 800, 854
BspTNI GGTCTC 1 cut(s) 338
BsrBI CCGCTC 1 cut(s) 255
BsrFI RCCGGY 5 cut(s) 14, 320, 368, 411, 817
BsrI ACTGG 1 cut(s) 37
BssAI RCCGGY 5 cut(s) 14, 320, 368, 411, 817
BssECI CCNNGG 4 cut(s) 154, 340, 374, 796
BssMI GATC 3 cut(s) 653, 745, 980
BssNI GRCGYC 4 cut(s) 24, 315, 713, 801
Bst2UI CCWGG 1 cut(s) 169
Bst4CI ACNGT 3 cut(s) 217, 608, 781
Bst6I CTCTTC 2 cut(s) 311, 734
BstACI GRCGYC 4 cut(s) 24, 315, 713, 801
BstAPI GCANNNNNTGC 2 cut(s) 57, 861
BstBAI YACGTR 1 cut(s) 557
BstC8I GCNNGC 3 cut(s) 143, 711, 819
BstDEI CTNAG 1 cut(s) 671
BstDSI CCRYGG 1 cut(s) 340
BstENI CCTNNNNNAGG 1 cut(s) 670
BstF5I GGATG 1 cut(s) 114
BstFNI CGCG 4 cut(s) 268, 270, 342, 955
BstH2I RGCGCY 3 cut(s) 318, 716, 804
BstHHI GCGC 5 cut(s) 44, 270, 317, 715, 803
BstKTI GATC 3 cut(s) 656, 748, 983
BstMAI GTCTC 3 cut(s) 196, 338, 496
BstMBI GATC 3 cut(s) 653, 745, 980
BstMCI CGRYCG 1 cut(s) 340
BstMWI GCNNNNNNNGC 4 cut(s) 48, 57, 686, 861
BstNI CCWGG 1 cut(s) 169
BstSCI CCNGG 9 cut(s) 75, 154, 167, 374, 473, 562, 594, 641, 684
BstSLI GKGCMC 1 cut(s) 632
BstUI CGCG 4 cut(s) 268, 270, 342, 955
BstV1I GCAGC 5 cut(s) 44, 146, 269, 774, 928
BstV2I GAAGAC 2 cut(s) 73, 179
BstZI CGGCCG 1 cut(s) 337
Bsu36I CCTNAGG 1 cut(s) 671
BsuRI GGCC 6 cut(s) 288, 303, 339, 458, 575, 689
BtgI CCRYGG 1 cut(s) 340
BtgZI GCGATG 1 cut(s) 1052
BtrI CACGTC 1 cut(s) 808
BtsCI GGATG 1 cut(s) 114
BtsIMutI CAGTG 1 cut(s) 44
BveI ACCTGC 1 cut(s) 185
Cac8I GCNNGC 3 cut(s) 143, 711, 819
CfoI GCGC 5 cut(s) 44, 270, 317, 715, 803
Cfr10I RCCGGY 5 cut(s) 14, 320, 368, 411, 817
Cfr13I GGNCC 4 cut(s) 4, 371, 414, 687
Cfr42I CCGCGG 1 cut(s) 343
CpoI CGGWCCG 1 cut(s) 414
CseI GACGC 2 cut(s) 259, 532
Csp6I GTAC 1 cut(s) 879
CspAI ACCGGT 2 cut(s) 368, 411
CspI CGGWCCG 1 cut(s) 414
CviAII CATG 1 cut(s) 48
CviQI GTAC 1 cut(s) 879
DdeI CTNAG 1 cut(s) 671
DinI GGCGCC 3 cut(s) 316, 714, 802
DpnI GATC 3 cut(s) 655, 747, 982
DpnII GATC 3 cut(s) 653, 745, 980
DraI TTTAAA 1 cut(s) 1053
EaeI YGGCCR 3 cut(s) 286, 337, 456
EagI CGGCCG 1 cut(s) 337
Eam1104I CTCTTC 2 cut(s) 311, 734
EarI CTCTTC 2 cut(s) 311, 734
EciI GGCGGA 2 cut(s) 239, 406
EclXI CGGCCG 1 cut(s) 337
Eco147I AGGCCT 1 cut(s) 303
Eco31I GGTCTC 1 cut(s) 338
Eco47I GGWCC 3 cut(s) 4, 371, 414
Eco52I CGGCCG 1 cut(s) 337
Eco57I CTGAAG 1 cut(s) 725
Eco72I CACGTG 1 cut(s) 557
Eco81I CCTNAGG 1 cut(s) 671
Eco88I CYCGRG 3 cut(s) 88, 625, 716
EcoNI CCTNNNNNAGG 1 cut(s) 670
EcoRII CCWGG 1 cut(s) 167
EgeI GGCGCC 3 cut(s) 316, 714, 802
EheI GGCGCC 3 cut(s) 316, 714, 802
Esp3I CGTCTC 1 cut(s) 496
FaeI CATG 1 cut(s) 51
FaiI YATR 5 cut(s) 49, 617, 899, 939, 975
FalI AAGNNNNNCTT 2 cut(s) 664, 696
FaqI GGGAC 3 cut(s) 248, 357, 565
FatI CATG 1 cut(s) 47
FauI CCCGC 8 cut(s) 273, 335, 542, 702, 868, 881, 940, 948
FblI GTMKAC 1 cut(s) 408
FokI GGATG 1 cut(s) 101
FspBI CTAG 1 cut(s) 1026
GlaI GCGC 5 cut(s) 43, 269, 316, 714, 802
GsuI CTGGAG 2 cut(s) 20, 151
HaeII RGCGCY 3 cut(s) 318, 716, 804
HaeIII GGCC 6 cut(s) 288, 303, 339, 458, 575, 689
HgaI GACGC 2 cut(s) 259, 532
HhaI GCGC 5 cut(s) 44, 270, 317, 715, 803
Hin1I GRCGYC 4 cut(s) 24, 315, 713, 801
Hin1II CATG 1 cut(s) 51
Hin6I GCGC 5 cut(s) 42, 268, 315, 713, 801
HinP1I GCGC 5 cut(s) 42, 268, 315, 713, 801
HincII GTYRAC 1 cut(s) 648
HindII GTYRAC 1 cut(s) 648
HinfI GANTC 4 cut(s) 452, 567, 623, 835
HphI GGTGA 2 cut(s) 205, 778
Hpy166II GTNNAC 3 cut(s) 409, 480, 648
Hpy188I TCNGA 3 cut(s) 22, 777, 797
Hpy188III TCNNGA 1 cut(s) 465
Hpy8I GTNNAC 3 cut(s) 409, 480, 648
HpyAV CCTTC 5 cut(s) 220, 256, 293, 763, 868
HpyCH4III ACNGT 3 cut(s) 217, 608, 781
HpyCH4IV ACGT 5 cut(s) 24, 512, 522, 556, 807
HpyCH4V TGCA 4 cut(s) 51, 162, 790, 1014
HpyF10VI GCNNNNNNNGC 4 cut(s) 48, 57, 686, 861
HpyF3I CTNAG 1 cut(s) 671
HpySE526I ACGT 5 cut(s) 24, 512, 522, 556, 807
Hsp92I GRCGYC 4 cut(s) 24, 315, 713, 801
Hsp92II CATG 1 cut(s) 51
HspAI GCGC 5 cut(s) 42, 268, 315, 713, 801
KasI GGCGCC 3 cut(s) 314, 712, 800
KroI GCCGGC 1 cut(s) 817
KroNI GCCGGC 1 cut(s) 819
KspI CCGCGG 1 cut(s) 343
Kzo9I GATC 3 cut(s) 653, 745, 980
LmnI GCTCC 6 cut(s) 23, 39, 128, 212, 260, 973
Lsp1109I GCAGC 5 cut(s) 44, 146, 269, 774, 928
MaeI CTAG 1 cut(s) 1026
MaeII ACGT 5 cut(s) 24, 512, 522, 556, 807
MaeIII GTNAC 1 cut(s) 637
MalI GATC 3 cut(s) 655, 747, 982
MbiI CCGCTC 1 cut(s) 255
MboI GATC 3 cut(s) 653, 745, 980
MboII GAAGA 4 cut(s) 73, 184, 298, 721
MhlI GDGCHC 1 cut(s) 632
MluCI AATT 2 cut(s) 389, 900
Mly113I GGCGCC 3 cut(s) 315, 713, 801
MlyI GAGTC 2 cut(s) 446, 617
MmeI TCCRAC 3 cut(s) 45, 519, 800
MroNI GCCGGC 1 cut(s) 817
MroXI GAANNNNTTC 2 cut(s) 680, 1006
MseI TTAA 2 cut(s) 1019, 1052
MslI CAYNNNNRTG 1 cut(s) 635
MspA1I CMGCKG 1 cut(s) 342
MspR9I CCNGG 9 cut(s) 77, 156, 169, 376, 475, 564, 596, 643, 686
Mva1269I GAATGC 1 cut(s) 682
MvaI CCWGG 1 cut(s) 169
MvnI CGCG 4 cut(s) 268, 270, 342, 955
MwoI GCNNNNNNNGC 4 cut(s) 48, 57, 686, 861
NaeI GCCGGC 1 cut(s) 819
NarI GGCGCC 3 cut(s) 315, 713, 801
NciI CCSGG 8 cut(s) 77, 156, 376, 475, 564, 596, 643, 686
NdeII GATC 3 cut(s) 653, 745, 980
NgoMIV GCCGGC 1 cut(s) 817
NlaIII CATG 1 cut(s) 51
NmeAIII GCCGAG 2 cut(s) 315, 434
NmuCI GTSAC 1 cut(s) 637
OliI CACNNNNGTG 1 cut(s) 635
PceI AGGCCT 1 cut(s) 303
PcsI WCGNNNNNNNCGW 4 cut(s) 69, 447, 540, 818
PctI GAATGC 1 cut(s) 682
PdiI GCCGGC 1 cut(s) 819
PdmI GAANNNNTTC 2 cut(s) 680, 1006
PfeI GAWTC 2 cut(s) 567, 835
PflFI GACNNNGTC 1 cut(s) 77
PfoI TCCNGGA 2 cut(s) 167, 562
PinAI ACCGGT 2 cut(s) 368, 411
PleI GAGTC 2 cut(s) 446, 617
PluTI GGCGCC 3 cut(s) 318, 716, 804
PmaCI CACGTG 1 cut(s) 557
PmlI CACGTG 1 cut(s) 557
PpsI GAGTC 2 cut(s) 446, 617
Ppu21I YACGTR 1 cut(s) 557
Psp6I CCWGG 1 cut(s) 167
PspCI CACGTG 1 cut(s) 557
PspGI CCWGG 1 cut(s) 167
PspPI GGNCC 4 cut(s) 4, 371, 414, 687
PsyI GACNNNGTC 1 cut(s) 77
RsaI GTAC 1 cut(s) 880
RsaNI GTAC 1 cut(s) 879
RseI CAYNNNNRTG 1 cut(s) 635
Rsr2I CGGWCCG 1 cut(s) 414
RsrII CGGWCCG 1 cut(s) 414
SacII CCGCGG 1 cut(s) 343
SaqAI TTAA 2 cut(s) 1019, 1052
Sau3AI GATC 3 cut(s) 653, 745, 980
Sau96I GGNCC 4 cut(s) 4, 371, 414, 687
SchI GAGTC 2 cut(s) 446, 617
ScrFI CCNGG 9 cut(s) 77, 156, 169, 376, 475, 564, 596, 643, 686
SduI GDGCHC 1 cut(s) 632
SfoI GGCGCC 3 cut(s) 316, 714, 802
Sfr303I CCGCGG 1 cut(s) 343
SgrBI CCGCGG 1 cut(s) 343
SinI GGWCC 3 cut(s) 4, 371, 414
SmiMI CAYNNNNRTG 1 cut(s) 635
Sse9I AATT 2 cut(s) 389, 900
SseBI AGGCCT 1 cut(s) 303
SspDI GGCGCC 3 cut(s) 314, 712, 800
SspI AATATT 1 cut(s) 1033
SspMI CTAG 1 cut(s) 1026
StuI AGGCCT 1 cut(s) 303
StyD4I CCNGG 9 cut(s) 75, 154, 167, 374, 473, 562, 594, 641, 684
TaaI ACNGT 3 cut(s) 217, 608, 781
TaiI ACGT 5 cut(s) 27, 515, 525, 559, 810
TaqI TCGA 8 cut(s) 9, 84, 147, 507, 525, 591, 621, 732
TasI AATT 2 cut(s) 389, 900
TauI GCSGC 6 cut(s) 141, 255, 288, 320, 342, 855
TfiI GAWTC 2 cut(s) 567, 835
Tru1I TTAA 2 cut(s) 1019, 1052
Tru9I TTAA 2 cut(s) 1019, 1052
TscAI CASTG 1 cut(s) 44
TseFI GTSAC 1 cut(s) 637
TseI GCWGC 5 cut(s) 57, 159, 282, 787, 916
Tsp45I GTSAC 1 cut(s) 637
TspGWI ACGGA 3 cut(s) 427, 625, 776
TspRI CASTG 1 cut(s) 44
Tth111I GACNNNGTC 1 cut(s) 77
VpaK11BI GGWCC 3 cut(s) 4, 371, 414
XagI CCTNNNNNAGG 1 cut(s) 670
XapI RAATTY 1 cut(s) 389
XmiI GTMKAC 1 cut(s) 408
XmnI GAANNNNTTC 2 cut(s) 680, 1006
XspI CTAG 1 cut(s) 1026
ZraI GACGTC 1 cut(s) 25
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.