MD07G1174200.v1.1

Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain II

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004407
Physical Location & Seq
Forward (+)
25215070 .. 25216533
1464 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1174200.v1.1.491

Sequence Viewer

Length: 477 bp
ATGACTCTTGAATATCTACAAAAAACAAGTCATTTGCCTTACAACAGGAATGGGTTCAAATTTTTCACAAATTCCGGAGGCCTTGGGAAACCTGACATTAAGAACATCTTTGAGCGTGCTGCAGATATATATGATAAATTTACGGTAGAAGGTTTGACAAACTCAAAACAAAAATTGCTTGCCTCCGTAAAGAGAGTTGATTGCATGAATGTATATACATCTGATCTTGTAAAGGCAGTCCGCAAAGCTGCAGGAAATATAGAGAAGCCACTGGAAGGGTTCCATATTGTAGTTGACAAAGGGAATGGAGCAGGAGGATTTTTCGCTGCAAAAGTGCTTGAACCTCTTGGTGCAGTCACTACTGGTAGTCAGTTCTTGGAGCCCGATGGTATGTTCCCAAATCATATACCGAACCCAGAGGACAAAACAGCTATGAAGGCTATCACCCAGGCAGTCCTTGATAACAAAGCTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PGM_PMM_I PGM_PMM_II PGM_PMM_III

Protein Analysis

159

Amino Acids

17.27

Weight (kDa)

8.59

Isoelectric Point (pI)

24.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGM_PMM_II PF02879 75 - 158 7.6e-12 Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 74
AciI CCGC 1 cut(s) 241
AcsI RAATTY 3 cut(s) 59, 70, 137
AfiI CCNNNNNNNGG 1 cut(s) 275
AgsI TTSAA 3 cut(s) 11, 58, 341
AjnI CCWGG 1 cut(s) 447
AluBI AGCT 3 cut(s) 248, 431, 470
AluI AGCT 3 cut(s) 248, 431, 470
Aor13HI TCCGGA 1 cut(s) 74
AoxI GGCC 1 cut(s) 79
ApeKI GCWGC 3 cut(s) 119, 248, 326
ApoI RAATTY 3 cut(s) 59, 70, 137
ArsI GACNNNNNNTTYG 2 cut(s) 13, 45
Asp700I GAANNNNTTC 1 cut(s) 53
AsuHPI GGTGA 1 cut(s) 436
BanII GRGCYC 1 cut(s) 384
BbvI GCAGC 3 cut(s) 106, 235, 313
BccI CCATC 1 cut(s) 380
BciT130I CCWGG 1 cut(s) 449
BfmI CTRYAG 2 cut(s) 120, 249
BisI GCNGC 3 cut(s) 120, 249, 327
BlsI GCNGC 3 cut(s) 121, 250, 328
Bme1390I CCNGG 1 cut(s) 449
BmiI GGNNCC 2 cut(s) 281, 381
BmrFI CCNGG 1 cut(s) 449
BsaJI CCNNGG 2 cut(s) 82, 447
BsaWI WCCGGW 1 cut(s) 74
Bsc4I CCNNNNNNNGG 1 cut(s) 275
Bse1I ACTGG 2 cut(s) 276, 367
BseAI TCCGGA 1 cut(s) 74
BseBI CCWGG 1 cut(s) 449
BseDI CCNNGG 2 cut(s) 82, 447
BseLI CCNNNNNNNGG 1 cut(s) 275
BseNI ACTGG 2 cut(s) 276, 367
BseXI GCAGC 3 cut(s) 106, 235, 313
BsgI GTGCAG 1 cut(s) 372
BshFI GGCC 1 cut(s) 81
BsiSI CCGG 1 cut(s) 75
BslI CCNNNNNNNGG 1 cut(s) 275
BsnI GGCC 1 cut(s) 81
Bsp1286I GDGCHC 1 cut(s) 384
Bsp13I TCCGGA 1 cut(s) 74
Bsp143I GATC 1 cut(s) 223
BspACI CCGC 1 cut(s) 241
BspANI GGCC 1 cut(s) 81
BspEI TCCGGA 1 cut(s) 74
BspLI GGNNCC 2 cut(s) 281, 381
BspMAI CTGCAG 2 cut(s) 124, 253
BsrI ACTGG 2 cut(s) 276, 367
BssECI CCNNGG 2 cut(s) 82, 447
BssMI GATC 1 cut(s) 223
BssT1I CCWWGG 1 cut(s) 82
Bst2UI CCWGG 1 cut(s) 449
Bst4CI ACNGT 1 cut(s) 145
BstC8I GCNNGC 2 cut(s) 117, 180
BstKTI GATC 1 cut(s) 226
BstMBI GATC 1 cut(s) 223
BstMWI GCNNNNNNNGC 1 cut(s) 437
BstNI CCWGG 1 cut(s) 449
BstSCI CCNGG 1 cut(s) 447
BstSFI CTRYAG 2 cut(s) 120, 249
BstV1I GCAGC 3 cut(s) 106, 235, 313
BsuRI GGCC 1 cut(s) 81
BtsIMutI CAGTG 1 cut(s) 269
Cac8I GCNNGC 2 cut(s) 117, 180
CviAII CATG 1 cut(s) 205
CviJI RGCY 7 cut(s) 81, 248, 268, 382, 431, 440, 470
CviKI_1 RGCY 7 cut(s) 81, 248, 268, 382, 431, 440, 470
DpnI GATC 1 cut(s) 225
DpnII GATC 1 cut(s) 223
Eco130I CCWWGG 1 cut(s) 82
Eco147I AGGCCT 1 cut(s) 81
Eco24I GRGCYC 1 cut(s) 384
EcoRII CCWGG 1 cut(s) 447
EcoT14I CCWWGG 1 cut(s) 82
EcoT38I GRGCYC 1 cut(s) 384
ErhI CCWWGG 1 cut(s) 82
FaeI CATG 1 cut(s) 208
FalI AAGNNNNNCTT 2 cut(s) 92, 124
FatI CATG 1 cut(s) 204
Fnu4HI GCNGC 3 cut(s) 120, 249, 327
FriOI GRGCYC 1 cut(s) 384
Fsp4HI GCNGC 3 cut(s) 120, 249, 327
GluI GCNGC 3 cut(s) 120, 249, 327
HaeIII GGCC 1 cut(s) 81
HapII CCGG 1 cut(s) 75
Hin1II CATG 1 cut(s) 208
HincII GTYRAC 1 cut(s) 295
HindII GTYRAC 1 cut(s) 295
HinfI GANTC 1 cut(s) 4
HpaII CCGG 1 cut(s) 75
HphI GGTGA 1 cut(s) 436
Hpy166II GTNNAC 1 cut(s) 295
Hpy188I TCNGA 1 cut(s) 223
Hpy188III TCNNGA 2 cut(s) 8, 75
Hpy8I GTNNAC 1 cut(s) 295
HpyAV CCTTC 3 cut(s) 143, 269, 430
HpyCH4III ACNGT 1 cut(s) 145
HpyCH4V TGCA 5 cut(s) 122, 204, 251, 329, 353
HpyF10VI GCNNNNNNNGC 1 cut(s) 437
Hsp92II CATG 1 cut(s) 208
Kpn2I TCCGGA 1 cut(s) 74
Kzo9I GATC 1 cut(s) 223
LmnI GCTCC 2 cut(s) 308, 379
Lsp1109I GCAGC 3 cut(s) 106, 235, 313
MaeIII GTNAC 1 cut(s) 355
MalI GATC 1 cut(s) 225
MboI GATC 1 cut(s) 223
MhlI GDGCHC 1 cut(s) 384
MluCI AATT 4 cut(s) 59, 70, 137, 173
MnlI CCTC 5 cut(s) 71, 193, 308, 354, 412
MroI TCCGGA 1 cut(s) 74
MroXI GAANNNNTTC 1 cut(s) 53
MseI TTAA 1 cut(s) 99
MspI CCGG 1 cut(s) 75
MspR9I CCNGG 1 cut(s) 449
MvaI CCWGG 1 cut(s) 449
MwoI GCNNNNNNNGC 1 cut(s) 437
NdeII GATC 1 cut(s) 223
NlaIII CATG 1 cut(s) 208
NlaIV GGNNCC 2 cut(s) 281, 381
NmuCI GTSAC 1 cut(s) 355
PceI AGGCCT 1 cut(s) 81
PdmI GAANNNNTTC 1 cut(s) 53
PkrI GCNGC 3 cut(s) 121, 250, 328
Psp6I CCWGG 1 cut(s) 447
PspGI CCWGG 1 cut(s) 447
PspN4I GGNNCC 2 cut(s) 281, 381
PstI CTGCAG 2 cut(s) 124, 253
SaqAI TTAA 1 cut(s) 99
SatI GCNGC 3 cut(s) 120, 249, 327
Sau3AI GATC 1 cut(s) 223
ScrFI CCNGG 1 cut(s) 449
SduI GDGCHC 1 cut(s) 384
SetI ASST 6 cut(s) 94, 154, 250, 346, 433, 472
SfcI CTRYAG 2 cut(s) 120, 249
Sse9I AATT 4 cut(s) 59, 70, 137, 173
SseBI AGGCCT 1 cut(s) 81
SsiI CCGC 1 cut(s) 241
StuI AGGCCT 1 cut(s) 81
StyD4I CCNGG 1 cut(s) 447
StyI CCWWGG 1 cut(s) 82
TaaI ACNGT 1 cut(s) 145
TasI AATT 4 cut(s) 59, 70, 137, 173
Tru1I TTAA 1 cut(s) 99
Tru9I TTAA 1 cut(s) 99
TscAI CASTG 1 cut(s) 276
TseFI GTSAC 1 cut(s) 355
TseI GCWGC 3 cut(s) 119, 248, 326
Tsp45I GTSAC 1 cut(s) 355
TspDTI ATGAA 2 cut(s) 221, 449
TspGWI ACGGA 1 cut(s) 175
TspRI CASTG 1 cut(s) 276
XapI RAATTY 3 cut(s) 59, 70, 137
XmnI GAANNNNTTC 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.