Rh1AG314700

Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain II

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0004407
Physical Location & Seq
Forward (+)
54751338 .. 54757478
6141 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG314700.1

Sequence Viewer

Length: 1392 bp
ATGATCAATTTCCAGGATGCAATTTCACGGGGAATTGCTGGTGCTGGTCTGGATGTTGTTCAATACGGATTGGCATCCACACCAGCAATGTTTAATAGCACAGTTACCGAAGATGATGGTTTCTTATGTCCTGCCGATGGGGCCATAATGATAACAGCAAGTCATTTGCCTTACAACAGGAATGGATTCAAATTCTTCACAAATGCTGGAGGGCTTGGGAAAGCTGACATTAAAAACATCTTAGAGCGTGCTGCAGATATTTACAATAAGTTTACAGTGGAAGGTTTGACAAGTTCAAAAGAAAAGGCACTTGCTTCTGTGAAGAGAGTTGATTATATGAACGTATATACATCTGATCTTGTAAAGGCAGTTCGCAAAGCTGCAGGAAATATAGAGAAGCCACTGGAAGGATTCCATATTGTTGTTGATGCAGGGAATGGAGCAGGAGGATTTTTTGCTGCAAAAGTGCTTGAACCTCTTGGGGCAGTCACTTCTGGTAGTCAGTTCTTGGAGCCAGATGGTATGTTTCCAAACCATATTCCAAACCCAGAGGACAAAGCAGCCATGAAAGCTATCACCCAGGCAGTCCTTGATAACAAGGCTGATTTAGGGATCATCTTTGATACAGACGTTGATAGATCTGCTGCTGTGGACTCTTCTGGCAACGAGTTCAATCGGAATCGGCTGATTGCCTTGATGTCTGCCATTGTTCTTGAGGAACATCCTGGAACAACTATTGTCACAGACAGTGTCACCTCAGATGGTCTTACCACATTCATCGAGCAGAAACTTGGGGGGAAGCACCACCGTTTCAAAAGAGGTTACAAAAATGTCATTGATGAAGCTATTCGTTTGAACTCTCTGGGTGAGGAATCACATCTGGCTATTGAAACTAGTGGCCATGGAGCTCTCAAGGAAAACCATTGGCTTGATGATGGTGCATACCTCATGGTCAAAATTTTAAATAAACTTGCCTCAGCTAGAGCTTCAGGACATGGTGGTGGCAGCAAAGTTTTGACTGATCTTTTAGAAGGTTTGGAGGAACCAGGTTTTTCAGTGGAACTGAGATTAAAGATCAATCAAAGTCATCAAGATCTTAAAGGAGGATCTTTCCGAGACTATGGAGAAGCAGTGTTGAAACATTTAGGAAACTATATTGAATCGTATCCTAAGCTTAAAAAAGCCCCTGTTAACCATGAAGGGGTACGAGTTTCTGGCTATGGTGGGTGGTTTCTTCTTAGACTCTCACTTCACGATCCTGTACTTCCCCTTAACATAGAGGCACCAAGCAAGAGTGATGCTGTATTACTTGGAAATGTTGTGCGTGCTGCTTTGACGGAGTTCTCTGCTTTGGATACATCTTCTTTAGATAAATTTGTCCAAGCATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PGM_PMM_I PGM_PMM_II PGM_PMM_III

Protein Analysis

463

Amino Acids

49.74

Weight (kDa)

5.8

Isoelectric Point (pI)

30.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGM_PMM_I PF02878 4 - 85 3.2e-14 Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain I
PGM_PMM_II PF02879 120 - 221 6.7e-21 Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain II
PGM_PMM_III PF02880 226 - 327 5.5e-10 Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1282
AclWI GGATC 3 cut(s) 620, 1114, 1250
AcoI YGGCCR 1 cut(s) 898
AcsI RAATTY 3 cut(s) 191, 957, 1373
AcuI CTGAAG 1 cut(s) 972
AfaI GTAC 2 cut(s) 1206, 1263
AfiI CCNNNNNNNGG 3 cut(s) 137, 407, 1201
AhlI ACTAGT 1 cut(s) 893
AjnI CCWGG 4 cut(s) 12, 579, 724, 1045
AluBI AGCT 8 cut(s) 224, 380, 572, 845, 908, 980, 986, 1174
AluI AGCT 8 cut(s) 224, 380, 572, 845, 908, 980, 986, 1174
Alw21I GWGCWC 1 cut(s) 910
Alw26I GTCTC 1 cut(s) 1110
AlwI GGATC 3 cut(s) 620, 1114, 1250
AoxI GGCC 2 cut(s) 141, 898
ApeKI GCWGC 7 cut(s) 251, 380, 458, 560, 644, 1005, 1328
ApoI RAATTY 3 cut(s) 191, 957, 1373
Asp700I GAANNNNTTC 2 cut(s) 185, 846
AspS9I GGNCC 1 cut(s) 141
AsuHPI GGTGA 3 cut(s) 568, 745, 878
BaeI ACNNNNGTAYC 2 cut(s) 615, 648
BalI TGGCCA 1 cut(s) 900
BanI GGYRCC 1 cut(s) 1282
BanII GRGCYC 1 cut(s) 910
Bbv12I GWGCWC 1 cut(s) 910
BbvCI CCTCAGC 1 cut(s) 976
BbvI GCAGC 7 cut(s) 238, 367, 445, 572, 631, 1017, 1315
BccI CCATC 5 cut(s) 110, 131, 512, 755, 929
BciT130I CCWGG 4 cut(s) 14, 581, 726, 1047
BciVI GTATCC 2 cut(s) 1176, 1348
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 1 cut(s) 1110
BcuI ACTAGT 1 cut(s) 893
BfaI CTAG 3 cut(s) 894, 981, 1390
BfmI CTRYAG 2 cut(s) 252, 381
BfuI GTATCC 2 cut(s) 1176, 1348
BglI GCCNNNNNGGC 1 cut(s) 140
BglII AGATCT 2 cut(s) 638, 1093
BisI GCNGC 7 cut(s) 252, 381, 459, 561, 645, 1006, 1329
BlsI GCNGC 7 cut(s) 253, 382, 460, 562, 646, 1007, 1330
Bme1390I CCNGG 4 cut(s) 14, 581, 726, 1047
BmgT120I GGNCC 1 cut(s) 141
BmiI GGNNCC 4 cut(s) 142, 513, 1044, 1284
BmrFI CCNGG 4 cut(s) 14, 581, 726, 1047
BmsI GCATC 4 cut(s) 7, 83, 418, 1288
BpmI CTGGAG 1 cut(s) 228
Bpu10I CCTNAGC 2 cut(s) 976, 1170
BpuEI CTTGAG 2 cut(s) 734, 896
BsaBI GATNNNNATC 1 cut(s) 73
BsaJI CCNNGG 2 cut(s) 579, 901
BsaXI ACNNNNNCTCC 2 cut(s) 1116, 1146
Bsc4I CCNNNNNNNGG 3 cut(s) 137, 407, 1201
Bse1I ACTGG 1 cut(s) 408
Bse3DI GCAATG 1 cut(s) 93
Bse8I GATNNNNATC 1 cut(s) 73
BseBI CCWGG 4 cut(s) 14, 581, 726, 1047
BseDI CCNNGG 2 cut(s) 579, 901
BseGI GGATG 5 cut(s) 22, 58, 74, 721, 1385
BseJI GATNNNNATC 1 cut(s) 73
BseLI CCNNNNNNNGG 3 cut(s) 137, 407, 1201
BseMI GCAATG 1 cut(s) 93
BseMII CTCAG 3 cut(s) 771, 990, 1055
BseNI ACTGG 1 cut(s) 408
BseXI GCAGC 7 cut(s) 238, 367, 445, 572, 631, 1017, 1315
BshFI GGCC 2 cut(s) 143, 900
BshNI GGYRCC 1 cut(s) 1282
BsiHKAI GWGCWC 1 cut(s) 910
BslI CCNNNNNNNGG 3 cut(s) 137, 407, 1201
BsmAI GTCTC 1 cut(s) 1110
BsnI GGCC 2 cut(s) 143, 900
Bsp1286I GDGCHC 1 cut(s) 910
Bsp143I GATC 9 cut(s) 3, 355, 612, 638, 1021, 1074, 1093, 1106, 1255
Bsp19I CCATGG 1 cut(s) 901
BspANI GGCC 2 cut(s) 143, 900
BspCNI CTCAG 3 cut(s) 770, 989, 1056
BspLI GGNNCC 4 cut(s) 142, 513, 1044, 1284
BspMAI CTGCAG 2 cut(s) 256, 385
BspPI GGATC 3 cut(s) 620, 1114, 1250
BspT107I GGYRCC 1 cut(s) 1282
BsrDI GCAATG 1 cut(s) 93
BsrI ACTGG 1 cut(s) 408
BssECI CCNNGG 2 cut(s) 579, 901
BssMI GATC 9 cut(s) 3, 355, 612, 638, 1021, 1074, 1093, 1106, 1255
BssT1I CCWWGG 1 cut(s) 901
Bst2UI CCWGG 4 cut(s) 14, 581, 726, 1047
Bst4CI ACNGT 4 cut(s) 103, 277, 749, 809
Bst6I CTCTTC 2 cut(s) 317, 661
BstC8I GCNNGC 2 cut(s) 249, 1326
BstDEI CTNAG 6 cut(s) 241, 757, 976, 1064, 1170, 1238
BstDSI CCRYGG 1 cut(s) 901
BstF5I GGATG 5 cut(s) 22, 58, 74, 721, 1385
BstKTI GATC 9 cut(s) 6, 358, 615, 641, 1024, 1077, 1096, 1109, 1258
BstMAI GTCTC 1 cut(s) 1110
BstMBI GATC 9 cut(s) 3, 355, 612, 638, 1021, 1074, 1093, 1106, 1255
BstMWI GCNNNNNNNGC 2 cut(s) 140, 569
BstNI CCWGG 4 cut(s) 14, 581, 726, 1047
BstSCI CCNGG 4 cut(s) 12, 579, 724, 1045
BstSFI CTRYAG 2 cut(s) 252, 381
BstV1I GCAGC 7 cut(s) 238, 367, 445, 572, 631, 1017, 1315
BstX2I RGATCY 3 cut(s) 638, 1093, 1106
BstYI RGATCY 3 cut(s) 638, 1093, 1106
BsuI GTATCC 2 cut(s) 1176, 1348
BsuRI GGCC 2 cut(s) 143, 900
BtgI CCRYGG 1 cut(s) 901
BtsCI GGATG 5 cut(s) 22, 58, 74, 721, 1385
BtsI GCAGTG 1 cut(s) 1137
BtsIMutI CAGTG 5 cut(s) 282, 401, 754, 1062, 1137
Cac8I GCNNGC 2 cut(s) 249, 1326
Cfr13I GGNCC 1 cut(s) 141
CsiI ACCWGGT 1 cut(s) 1045
Csp6I GTAC 2 cut(s) 1205, 1262
CviAII CATG 5 cut(s) 565, 902, 949, 995, 1196
CviQI GTAC 2 cut(s) 1205, 1262
DdeI CTNAG 6 cut(s) 241, 757, 976, 1064, 1170, 1238
DpnI GATC 9 cut(s) 5, 357, 614, 640, 1023, 1076, 1095, 1108, 1257
DpnII GATC 9 cut(s) 3, 355, 612, 638, 1021, 1074, 1093, 1106, 1255
DraI TTTAAA 1 cut(s) 963
EaeI YGGCCR 1 cut(s) 898
Eam1104I CTCTTC 2 cut(s) 317, 661
EarI CTCTTC 2 cut(s) 317, 661
Ecl136II GAGCTC 1 cut(s) 908
Eco130I CCWWGG 1 cut(s) 901
Eco24I GRGCYC 1 cut(s) 910
Eco53kI GAGCTC 1 cut(s) 908
Eco57I CTGAAG 1 cut(s) 972
EcoICRI GAGCTC 1 cut(s) 908
EcoRII CCWGG 4 cut(s) 12, 579, 724, 1045
EcoT14I CCWWGG 1 cut(s) 901
EcoT38I GRGCYC 1 cut(s) 910
ErhI CCWWGG 1 cut(s) 901
FaeI CATG 5 cut(s) 568, 905, 952, 998, 1199
FatI CATG 5 cut(s) 564, 901, 948, 994, 1195
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 7 cut(s) 252, 381, 459, 561, 645, 1006, 1329
FokI GGATG 5 cut(s) 29, 61, 65, 708, 1372
FriOI GRGCYC 1 cut(s) 910
Fsp4HI GCNGC 7 cut(s) 252, 381, 459, 561, 645, 1006, 1329
FspBI CTAG 3 cut(s) 894, 981, 1390
GluI GCNGC 7 cut(s) 252, 381, 459, 561, 645, 1006, 1329
GsuI CTGGAG 1 cut(s) 228
HaeIII GGCC 2 cut(s) 143, 900
Hin1II CATG 5 cut(s) 568, 905, 952, 998, 1199
HincII GTYRAC 1 cut(s) 1192
HindII GTYRAC 1 cut(s) 1192
HindIII AAGCTT 1 cut(s) 1172
HinfI GANTC 7 cut(s) 186, 411, 653, 679, 872, 1160, 1242
HpaI GTTAAC 1 cut(s) 1192
HphI GGTGA 3 cut(s) 568, 745, 878
Hpy166II GTNNAC 3 cut(s) 273, 652, 1192
Hpy188I TCNGA 4 cut(s) 355, 678, 760, 1115
Hpy188III TCNNGA 5 cut(s) 50, 713, 990, 1091, 1253
Hpy8I GTNNAC 3 cut(s) 273, 652, 1192
HpyAV CCTTC 4 cut(s) 275, 401, 1025, 1193
HpyCH4III ACNGT 4 cut(s) 103, 277, 749, 809
HpyCH4IV ACGT 2 cut(s) 342, 630
HpyCH4V TGCA 6 cut(s) 20, 254, 383, 431, 461, 941
HpyF10VI GCNNNNNNNGC 2 cut(s) 140, 569
HpyF3I CTNAG 6 cut(s) 241, 757, 976, 1064, 1170, 1238
HpySE526I ACGT 2 cut(s) 342, 630
Hsp92II CATG 5 cut(s) 568, 905, 952, 998, 1199
Ksp22I TGATCA 1 cut(s) 3
KspAI GTTAAC 1 cut(s) 1192
Kzo9I GATC 9 cut(s) 3, 355, 612, 638, 1021, 1074, 1093, 1106, 1255
LmnI GCTCC 3 cut(s) 440, 511, 905
Lsp1109I GCAGC 7 cut(s) 238, 367, 445, 572, 631, 1017, 1315
LweI GCATC 4 cut(s) 7, 83, 418, 1288
MabI ACCWGGT 1 cut(s) 1045
MaeI CTAG 3 cut(s) 894, 981, 1390
MaeII ACGT 2 cut(s) 342, 630
MaeIII GTNAC 5 cut(s) 103, 487, 739, 751, 821
MalI GATC 9 cut(s) 5, 357, 614, 640, 1023, 1076, 1095, 1108, 1257
MboI GATC 9 cut(s) 3, 355, 612, 638, 1021, 1074, 1093, 1106, 1255
MboII GAAGA 6 cut(s) 122, 187, 334, 648, 1226, 1353
MflI RGATCY 3 cut(s) 638, 1093, 1106
MhlI GDGCHC 1 cut(s) 910
MlsI TGGCCA 1 cut(s) 900
MluCI AATT 6 cut(s) 7, 21, 33, 191, 957, 1373
MluNI TGGCCA 1 cut(s) 900
MlyI GAGTC 2 cut(s) 647, 1236
Mox20I TGGCCA 1 cut(s) 900
MroXI GAANNNNTTC 2 cut(s) 185, 846
MscI TGGCCA 1 cut(s) 900
MseI TTAA 8 cut(s) 93, 231, 962, 1070, 1098, 1176, 1191, 1272
MslI CAYNNNNRTG 1 cut(s) 999
Msp20I TGGCCA 1 cut(s) 900
MspR9I CCNGG 4 cut(s) 14, 581, 726, 1047
MvaI CCWGG 4 cut(s) 14, 581, 726, 1047
MwoI GCNNNNNNNGC 2 cut(s) 140, 569
NcoI CCATGG 1 cut(s) 901
NdeII GATC 9 cut(s) 3, 355, 612, 638, 1021, 1074, 1093, 1106, 1255
NlaIII CATG 5 cut(s) 568, 905, 952, 998, 1199
NlaIV GGNNCC 4 cut(s) 142, 513, 1044, 1284
NmuCI GTSAC 3 cut(s) 487, 739, 751
PdmI GAANNNNTTC 2 cut(s) 185, 846
PfeI GAWTC 5 cut(s) 186, 411, 679, 872, 1160
PflFI GACNNNGTC 1 cut(s) 749
PfoI TCCNGGA 2 cut(s) 12, 724
PkrI GCNGC 7 cut(s) 253, 382, 460, 562, 646, 1007, 1330
PleI GAGTC 2 cut(s) 647, 1236
PpsI GAGTC 2 cut(s) 647, 1236
Psp124BI GAGCTC 1 cut(s) 910
Psp6I CCWGG 4 cut(s) 12, 579, 724, 1045
PspGI CCWGG 4 cut(s) 12, 579, 724, 1045
PspN4I GGNNCC 4 cut(s) 142, 513, 1044, 1284
PspPI GGNCC 1 cut(s) 141
PsrI GAACNNNNNNTAC 2 cut(s) 354, 386
PstI CTGCAG 2 cut(s) 256, 385
PsuI RGATCY 3 cut(s) 638, 1093, 1106
PsyI GACNNNGTC 1 cut(s) 749
RsaI GTAC 2 cut(s) 1206, 1263
RsaNI GTAC 2 cut(s) 1205, 1262
RseI CAYNNNNRTG 1 cut(s) 999
SacI GAGCTC 1 cut(s) 910
SaqAI TTAA 8 cut(s) 93, 231, 962, 1070, 1098, 1176, 1191, 1272
SatI GCNGC 7 cut(s) 252, 381, 459, 561, 645, 1006, 1329
Sau3AI GATC 9 cut(s) 3, 355, 612, 638, 1021, 1074, 1093, 1106, 1255
Sau96I GGNCC 1 cut(s) 141
SchI GAGTC 2 cut(s) 647, 1236
ScrFI CCNGG 4 cut(s) 14, 581, 726, 1047
SduI GDGCHC 1 cut(s) 910
SexAI ACCWGGT 1 cut(s) 1045
SfaNI GCATC 4 cut(s) 7, 83, 418, 1288
SfcI CTRYAG 2 cut(s) 252, 381
SmiMI CAYNNNNRTG 1 cut(s) 999
SmlI CTYRAG 2 cut(s) 713, 911
SmoI CTYRAG 2 cut(s) 713, 911
SpeI ACTAGT 1 cut(s) 893
Sse9I AATT 6 cut(s) 7, 21, 33, 191, 957, 1373
SspMI CTAG 3 cut(s) 894, 981, 1390
SstI GAGCTC 1 cut(s) 910
StyD4I CCNGG 4 cut(s) 12, 579, 724, 1045
StyI CCWWGG 1 cut(s) 901
TaaI ACNGT 4 cut(s) 103, 277, 749, 809
TaiI ACGT 2 cut(s) 345, 633
TaqI TCGA 1 cut(s) 780
TasI AATT 6 cut(s) 7, 21, 33, 191, 957, 1373
TatI WGTACW 1 cut(s) 1261
TfiI GAWTC 5 cut(s) 186, 411, 679, 872, 1160
Tru1I TTAA 8 cut(s) 93, 231, 962, 1070, 1098, 1176, 1191, 1272
Tru9I TTAA 8 cut(s) 93, 231, 962, 1070, 1098, 1176, 1191, 1272
TscAI CASTG 5 cut(s) 282, 408, 754, 1062, 1137
TseFI GTSAC 3 cut(s) 487, 739, 751
TseI GCWGC 7 cut(s) 251, 380, 458, 560, 644, 1005, 1328
Tsp45I GTSAC 3 cut(s) 487, 739, 751
TspDTI ATGAA 5 cut(s) 353, 581, 766, 855, 1212
TspGWI ACGGA 2 cut(s) 81, 1352
TspRI CASTG 5 cut(s) 282, 408, 754, 1062, 1137
Tth111I GACNNNGTC 1 cut(s) 749
XapI RAATTY 3 cut(s) 191, 957, 1373
XmnI GAANNNNTTC 2 cut(s) 185, 846
XspI CTAG 3 cut(s) 894, 981, 1390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.