Rw1G027890

Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain II

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0004407
Physical Location & Seq
Forward (+)
55397826 .. 55403139
5314 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G027890.1

Sequence Viewer

Length: 1827 bp
ATGGAAGCAATGTCAGGGAAGATTGTCGAAAATGTTACTGTGTCACAATGCTACCAACTAAGTAGGAAGTTCGACAACCAATATCGAAGGGACCATTGTGCCCCTTTTATGCGCAAAGTGCTTCCCTTACAGCAAGGGAAGTTAGCATGGACTGCCATTCATTCCATGCAATTGCGCAACTTATCAAAATACCAGAGCAGTTTTGTAAAGCGAAGAACTATTCATTGCAATGCTGCTGCATCTACCACTGCAGTTCCATATCTTGAAAAAGTTGATTTTTTGAAGCTTCAAAATGGCAGTGATATTCGAGGTGTTGCTGTTGCTGGGGTTGAAGGAGAGCCAATCAATTTGACTGAACCTGTGGCAGAAGCAATAGCAGCTGGTTTTGCTGCATGGTTGGCGGAAAAGAAGAAGGCCGACTCTAAACGTTTGAGAGTTTCTGTTGGCCATGATTCTCGAATATCTGCACAACAGTTAGAGGTATGTCCTAAATGTAACAGCATATTGGCATCCACACCAGCAATGTTTAATAGCACAGTTACCGAAGATGATGGTTTCTTATGTCCTGCCGATGGGGCCATAATGATAACAGCAAGTCATTTGCCTTACAACAGGAATGGATTCAAATTCTTCACAAATGCTGGAGGGCTTGGGAAAGCTGACATTAAAAACATCTTAGAGCGTGCTGCAGATATTTACAATAAGTTTACAGTGGAAGGTTTGACAAGTTCAAAAGAAAAGGCACTTACTTCTGTGAAGAGAGTTGATTATATGAACGTATATACATCTGATCTTGTAAAGGCAGTTCGCAAAGCTGCAGGAAATATAGAGAAGCCACTGGAAGGATTCCATATTGTTGTTGATGCAGGGAATGGAGCAGGAGGATTTTTTGCTGCAAAAGTGCTTGAACCTCTTGGGGCAGTCACTTCTGGTAGTCAGTTCTTGGAGCCAGATGGTATGTTTCCAAACCATATTCCAAACCCAGAGGACAAAGCAGCCATGAAAGCTATCACCCAGGCAGTCCTTGATAACAAGGCTGATTTAGGGATCATCTTTGATACAGACGTTGATAGATCTGCTGCTGTGGACTCTTCTGGCAACGAGTTCAATCGGAATCGGCTGATTGCCTTGATGTCTGCCATTGTTCTTGAGGAACATCCTGGAACAACTATTGTCACAGACAGTGTCACCTCAGATGGTCTTACCACATTCATCGAGCAGAAACTTGGGGGGAAGCACCACCGTTTCAAAAGAGGTTACAAAAATGTCATTGATGAAGCTATTCGTTTGAACTCTCTGGGTGAGGAATCACATCTGGCTATTGAAACTAGTGGCCATGGAGCTCTCAAGGAAAACCATTGGCTTGATGATGGTGCATACCTCATGGTCAAAATTTTAAATAAACTTGCCTCAGCTAGAGCTTCAGGACATGGTGGTGGCAGCAAAGTTTTGACTGATCTTTTAGAAGGTTTGGAGGAACCAGGTTTTTCAGTGGAACTGAGATTAAAGATCAATCAAAGTCATCAAGATCTTAAAGGAGGATCTTTCCGAGACTATGGAGAAGCAGTGTTGAAACATTTAGGAAACTATATTGAATCGTATCCTAAGCTTAAAAAAGCCCCTGTTAACCATGAAGGGGTACGAGTTTCTGGCTATGGTGGGTGGTTTCTTCTTAGACTCTCACTTCACGATCCTGTACTTCCCCTTAACATAGAGGCACCAAGCAAGAGTGATGCTGTATTACTTGGAAATGTTGTGCGTGCTGCTTTGACGGAGTTCTCTGCTTTGGATACATCTTCTTTAGATAAATTTGTCCAAGCATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PGM_PMM_I PGM_PMM_II PGM_PMM_III

Protein Analysis

608

Amino Acids

66.04

Weight (kDa)

7.3

Isoelectric Point (pI)

35.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGM_PMM_I PF02878 98 - 230 2e-13 Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain I
PGM_PMM_II PF02879 265 - 366 1.1e-20 Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain II
PGM_PMM_III PF02880 371 - 472 8.7e-10 Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 113, 176
AccB1I GGYRCC 1 cut(s) 1717
AciI CCGC 1 cut(s) 401
AclI AACGTT 1 cut(s) 427
AclWI GGATC 3 cut(s) 1055, 1549, 1685
AcoI YGGCCR 2 cut(s) 445, 1333
AcsI RAATTY 3 cut(s) 626, 1392, 1808
AcuI CTGAAG 1 cut(s) 1407
AfaI GTAC 2 cut(s) 1641, 1698
AfiI CCNNNNNNNGG 3 cut(s) 572, 842, 1636
AhlI ACTAGT 1 cut(s) 1328
AjnI CCWGG 3 cut(s) 1014, 1159, 1480
Alw21I GWGCWC 1 cut(s) 1345
Alw26I GTCTC 1 cut(s) 1545
AlwI GGATC 3 cut(s) 1055, 1549, 1685
AoxI GGCC 4 cut(s) 414, 445, 576, 1333
ApoI RAATTY 3 cut(s) 626, 1392, 1808
Asp700I GAANNNNTTC 2 cut(s) 620, 1281
AspLEI GCGC 2 cut(s) 114, 177
AspS9I GGNCC 2 cut(s) 91, 576
AsuHPI GGTGA 3 cut(s) 1003, 1180, 1313
AvaII GGWCC 1 cut(s) 91
BaeGI GKGCMC 1 cut(s) 103
BaeI ACNNNNGTAYC 2 cut(s) 1050, 1083
BalI TGGCCA 2 cut(s) 447, 1335
BanI GGYRCC 1 cut(s) 1717
BanII GRGCYC 1 cut(s) 1345
Bbv12I GWGCWC 1 cut(s) 1345
BbvCI CCTCAGC 1 cut(s) 1411
BccI CCATC 5 cut(s) 545, 566, 947, 1190, 1364
BciT130I CCWGG 3 cut(s) 1016, 1161, 1482
BciVI GTATCC 2 cut(s) 1611, 1783
BcoDI GTCTC 1 cut(s) 1545
BcuI ACTAGT 1 cut(s) 1328
BfaI CTAG 3 cut(s) 1329, 1416, 1825
BfmI CTRYAG 3 cut(s) 249, 687, 816
BfuI GTATCC 2 cut(s) 1611, 1783
BglI GCCNNNNNGGC 1 cut(s) 575
BglII AGATCT 2 cut(s) 1073, 1528
Bme1390I CCNGG 3 cut(s) 1016, 1161, 1482
Bme18I GGWCC 1 cut(s) 91
BmgT120I GGNCC 2 cut(s) 91, 576
BmiI GGNNCC 5 cut(s) 92, 577, 948, 1479, 1719
BmrFI CCNGG 3 cut(s) 1016, 1161, 1482
BmsI GCATC 4 cut(s) 248, 518, 853, 1723
BpmI CTGGAG 1 cut(s) 663
Bpu10I CCTNAGC 2 cut(s) 1411, 1605
BpuEI CTTGAG 2 cut(s) 1169, 1331
BsaJI CCNNGG 2 cut(s) 1014, 1336
BsaXI ACNNNNNCTCC 2 cut(s) 1551, 1581
Bsc4I CCNNNNNNNGG 3 cut(s) 572, 842, 1636
Bse1I ACTGG 1 cut(s) 843
Bse3DI GCAATG 4 cut(s) 15, 223, 235, 528
BseBI CCWGG 3 cut(s) 1016, 1161, 1482
BseDI CCNNGG 2 cut(s) 1014, 1336
BseGI GGATG 3 cut(s) 509, 1156, 1820
BseLI CCNNNNNNNGG 3 cut(s) 572, 842, 1636
BseMI GCAATG 4 cut(s) 15, 223, 235, 528
BseMII CTCAG 3 cut(s) 1206, 1425, 1490
BseNI ACTGG 1 cut(s) 843
BseSI GKGCMC 1 cut(s) 103
BseYI CCCAGC 1 cut(s) 323
BsgI GTGCAG 1 cut(s) 450
BshFI GGCC 4 cut(s) 416, 447, 578, 1335
BshNI GGYRCC 1 cut(s) 1717
BsiHKAI GWGCWC 1 cut(s) 1345
BslFI GGGAC 1 cut(s) 104
BslI CCNNNNNNNGG 3 cut(s) 572, 842, 1636
BsmAI GTCTC 1 cut(s) 1545
BsmFI GGGAC 1 cut(s) 104
BsnI GGCC 4 cut(s) 416, 447, 578, 1335
Bsp1286I GDGCHC 2 cut(s) 103, 1345
Bsp143I GATC 8 cut(s) 790, 1047, 1073, 1456, 1509, 1528, 1541, 1690
Bsp19I CCATGG 1 cut(s) 1336
BspACI CCGC 1 cut(s) 401
BspANI GGCC 4 cut(s) 416, 447, 578, 1335
BspCNI CTCAG 3 cut(s) 1205, 1424, 1491
BspLI GGNNCC 5 cut(s) 92, 577, 948, 1479, 1719
BspMAI CTGCAG 3 cut(s) 253, 691, 820
BspPI GGATC 3 cut(s) 1055, 1549, 1685
BspT107I GGYRCC 1 cut(s) 1717
BsrDI GCAATG 4 cut(s) 15, 223, 235, 528
BsrI ACTGG 1 cut(s) 843
BssECI CCNNGG 2 cut(s) 1014, 1336
BssMI GATC 8 cut(s) 790, 1047, 1073, 1456, 1509, 1528, 1541, 1690
BssT1I CCWWGG 1 cut(s) 1336
Bst2UI CCWGG 3 cut(s) 1016, 1161, 1482
Bst4CI ACNGT 6 cut(s) 40, 474, 538, 712, 1184, 1244
Bst6I CTCTTC 2 cut(s) 752, 1096
BstAPI GCANNNNNTGC 1 cut(s) 152
BstC8I GCNNGC 2 cut(s) 684, 1761
BstDEI CTNAG 7 cut(s) 59, 676, 1192, 1411, 1499, 1605, 1673
BstDSI CCRYGG 1 cut(s) 1336
BstF5I GGATG 3 cut(s) 509, 1156, 1820
BstHHI GCGC 2 cut(s) 114, 177
BstKTI GATC 8 cut(s) 793, 1050, 1076, 1459, 1512, 1531, 1544, 1693
BstMAI GTCTC 1 cut(s) 1545
BstMBI GATC 8 cut(s) 790, 1047, 1073, 1456, 1509, 1528, 1541, 1690
BstMWI GCNNNNNNNGC 7 cut(s) 118, 152, 377, 386, 398, 575, 1004
BstNI CCWGG 3 cut(s) 1016, 1161, 1482
BstSCI CCNGG 3 cut(s) 1014, 1159, 1480
BstSFI CTRYAG 3 cut(s) 249, 687, 816
BstSLI GKGCMC 1 cut(s) 103
BstX2I RGATCY 3 cut(s) 1073, 1528, 1541
BstYI RGATCY 3 cut(s) 1073, 1528, 1541
BsuI GTATCC 2 cut(s) 1611, 1783
BsuRI GGCC 4 cut(s) 416, 447, 578, 1335
BtgI CCRYGG 1 cut(s) 1336
BtsCI GGATG 3 cut(s) 509, 1156, 1820
BtsI GCAGTG 3 cut(s) 246, 304, 1572
BtsIMutI CAGTG 7 cut(s) 246, 304, 717, 836, 1189, 1497, 1572
Cac8I GCNNGC 2 cut(s) 684, 1761
CfoI GCGC 2 cut(s) 114, 177
Cfr13I GGNCC 2 cut(s) 91, 576
CsiI ACCWGGT 1 cut(s) 1480
Csp6I GTAC 2 cut(s) 1640, 1697
CviAII CATG 9 cut(s) 147, 166, 393, 449, 1000, 1337, 1384, 1430, 1631
CviQI GTAC 2 cut(s) 1640, 1697
DdeI CTNAG 7 cut(s) 59, 676, 1192, 1411, 1499, 1605, 1673
DpnI GATC 8 cut(s) 792, 1049, 1075, 1458, 1511, 1530, 1543, 1692
DpnII GATC 8 cut(s) 790, 1047, 1073, 1456, 1509, 1528, 1541, 1690
DraI TTTAAA 1 cut(s) 1398
EaeI YGGCCR 2 cut(s) 445, 1333
Eam1104I CTCTTC 2 cut(s) 752, 1096
EarI CTCTTC 2 cut(s) 752, 1096
EciI GGCGGA 1 cut(s) 416
Ecl136II GAGCTC 1 cut(s) 1343
Eco130I CCWWGG 1 cut(s) 1336
Eco24I GRGCYC 1 cut(s) 1345
Eco47I GGWCC 1 cut(s) 91
Eco53kI GAGCTC 1 cut(s) 1343
Eco57I CTGAAG 1 cut(s) 1407
EcoICRI GAGCTC 1 cut(s) 1343
EcoRII CCWGG 3 cut(s) 1014, 1159, 1480
EcoT14I CCWWGG 1 cut(s) 1336
EcoT38I GRGCYC 1 cut(s) 1345
ErhI CCWWGG 1 cut(s) 1336
FaeI CATG 9 cut(s) 150, 169, 396, 452, 1003, 1340, 1387, 1433, 1634
FaqI GGGAC 1 cut(s) 104
FatI CATG 9 cut(s) 146, 165, 392, 448, 999, 1336, 1383, 1429, 1630
FokI GGATG 3 cut(s) 496, 1143, 1807
FriOI GRGCYC 1 cut(s) 1345
FspBI CTAG 3 cut(s) 1329, 1416, 1825
FspI TGCGCA 2 cut(s) 113, 176
GlaI GCGC 2 cut(s) 113, 176
GsaI CCCAGC 1 cut(s) 327
GsuI CTGGAG 1 cut(s) 663
HaeIII GGCC 4 cut(s) 416, 447, 578, 1335
HhaI GCGC 2 cut(s) 114, 177
Hin1II CATG 9 cut(s) 150, 169, 396, 452, 1003, 1340, 1387, 1433, 1634
Hin6I GCGC 2 cut(s) 112, 175
HinP1I GCGC 2 cut(s) 112, 175
HincII GTYRAC 1 cut(s) 1627
HindII GTYRAC 1 cut(s) 1627
HindIII AAGCTT 2 cut(s) 284, 1607
HinfI GANTC 9 cut(s) 419, 452, 621, 846, 1088, 1114, 1307, 1595, 1677
HpaI GTTAAC 1 cut(s) 1627
HphI GGTGA 3 cut(s) 1003, 1180, 1313
Hpy166II GTNNAC 3 cut(s) 708, 1087, 1627
Hpy188I TCNGA 4 cut(s) 790, 1113, 1195, 1550
Hpy188III TCNNGA 6 cut(s) 263, 456, 1148, 1425, 1526, 1688
Hpy8I GTNNAC 3 cut(s) 708, 1087, 1627
HpyAV CCTTC 7 cut(s) 81, 326, 406, 710, 836, 1460, 1628
HpyCH4III ACNGT 6 cut(s) 40, 474, 538, 712, 1184, 1244
HpyCH4IV ACGT 3 cut(s) 427, 777, 1065
HpyF10VI GCNNNNNNNGC 7 cut(s) 118, 152, 377, 386, 398, 575, 1004
HpyF3I CTNAG 7 cut(s) 59, 676, 1192, 1411, 1499, 1605, 1673
HpySE526I ACGT 3 cut(s) 427, 777, 1065
Hsp92II CATG 9 cut(s) 150, 169, 396, 452, 1003, 1340, 1387, 1433, 1634
HspAI GCGC 2 cut(s) 112, 175
KspAI GTTAAC 1 cut(s) 1627
Kzo9I GATC 8 cut(s) 790, 1047, 1073, 1456, 1509, 1528, 1541, 1690
LmnI GCTCC 3 cut(s) 875, 946, 1340
LweI GCATC 4 cut(s) 248, 518, 853, 1723
MabI ACCWGGT 1 cut(s) 1480
MaeI CTAG 3 cut(s) 1329, 1416, 1825
MaeII ACGT 3 cut(s) 427, 777, 1065
MaeIII GTNAC 8 cut(s) 34, 42, 494, 538, 922, 1174, 1186, 1256
MalI GATC 8 cut(s) 792, 1049, 1075, 1458, 1511, 1530, 1543, 1692
MboI GATC 8 cut(s) 790, 1047, 1073, 1456, 1509, 1528, 1541, 1690
MboII GAAGA 9 cut(s) 31, 225, 421, 557, 622, 769, 1083, 1661, 1788
MfeI CAATTG 1 cut(s) 170
MflI RGATCY 3 cut(s) 1073, 1528, 1541
MhlI GDGCHC 2 cut(s) 103, 1345
MlsI TGGCCA 2 cut(s) 447, 1335
MluCI AATT 5 cut(s) 170, 346, 626, 1392, 1808
MluNI TGGCCA 2 cut(s) 447, 1335
MlyI GAGTC 3 cut(s) 413, 1082, 1671
Mox20I TGGCCA 2 cut(s) 447, 1335
MroXI GAANNNNTTC 2 cut(s) 620, 1281
MscI TGGCCA 2 cut(s) 447, 1335
MseI TTAA 8 cut(s) 528, 666, 1397, 1505, 1533, 1611, 1626, 1707
MslI CAYNNNNRTG 2 cut(s) 228, 1434
Msp20I TGGCCA 2 cut(s) 447, 1335
MspA1I CMGCKG 1 cut(s) 380
MspR9I CCNGG 3 cut(s) 1016, 1161, 1482
MunI CAATTG 1 cut(s) 170
MvaI CCWGG 3 cut(s) 1016, 1161, 1482
MwoI GCNNNNNNNGC 7 cut(s) 118, 152, 377, 386, 398, 575, 1004
NcoI CCATGG 1 cut(s) 1336
NdeII GATC 8 cut(s) 790, 1047, 1073, 1456, 1509, 1528, 1541, 1690
NlaIII CATG 9 cut(s) 150, 169, 396, 452, 1003, 1340, 1387, 1433, 1634
NlaIV GGNNCC 5 cut(s) 92, 577, 948, 1479, 1719
NmuCI GTSAC 4 cut(s) 42, 922, 1174, 1186
NsbI TGCGCA 2 cut(s) 113, 176
PdmI GAANNNNTTC 2 cut(s) 620, 1281
PfeI GAWTC 6 cut(s) 452, 621, 846, 1114, 1307, 1595
PflFI GACNNNGTC 1 cut(s) 1184
PfoI TCCNGGA 1 cut(s) 1159
PleI GAGTC 3 cut(s) 413, 1082, 1671
PpsI GAGTC 3 cut(s) 413, 1082, 1671
Psp124BI GAGCTC 1 cut(s) 1345
Psp1406I AACGTT 1 cut(s) 427
Psp6I CCWGG 3 cut(s) 1014, 1159, 1480
PspFI CCCAGC 1 cut(s) 323
PspGI CCWGG 3 cut(s) 1014, 1159, 1480
PspN4I GGNNCC 5 cut(s) 92, 577, 948, 1479, 1719
PspPI GGNCC 2 cut(s) 91, 576
PsrI GAACNNNNNNTAC 2 cut(s) 789, 821
PstI CTGCAG 3 cut(s) 253, 691, 820
PsuI RGATCY 3 cut(s) 1073, 1528, 1541
PsyI GACNNNGTC 1 cut(s) 1184
PvuII CAGCTG 1 cut(s) 380
RsaI GTAC 2 cut(s) 1641, 1698
RsaNI GTAC 2 cut(s) 1640, 1697
RseI CAYNNNNRTG 2 cut(s) 228, 1434
SacI GAGCTC 1 cut(s) 1345
SaqAI TTAA 8 cut(s) 528, 666, 1397, 1505, 1533, 1611, 1626, 1707
Sau3AI GATC 8 cut(s) 790, 1047, 1073, 1456, 1509, 1528, 1541, 1690
Sau96I GGNCC 2 cut(s) 91, 576
SchI GAGTC 3 cut(s) 413, 1082, 1671
ScrFI CCNGG 3 cut(s) 1016, 1161, 1482
SduI GDGCHC 2 cut(s) 103, 1345
SexAI ACCWGGT 1 cut(s) 1480
SfaNI GCATC 4 cut(s) 248, 518, 853, 1723
SfcI CTRYAG 3 cut(s) 249, 687, 816
SinI GGWCC 1 cut(s) 91
SmiMI CAYNNNNRTG 2 cut(s) 228, 1434
SmlI CTYRAG 2 cut(s) 1148, 1346
SmoI CTYRAG 2 cut(s) 1148, 1346
SpeI ACTAGT 1 cut(s) 1328
Sse9I AATT 5 cut(s) 170, 346, 626, 1392, 1808
SsiI CCGC 1 cut(s) 401
SspMI CTAG 3 cut(s) 1329, 1416, 1825
SstI GAGCTC 1 cut(s) 1345
StyD4I CCNGG 3 cut(s) 1014, 1159, 1480
StyI CCWWGG 1 cut(s) 1336
TaaI ACNGT 6 cut(s) 40, 474, 538, 712, 1184, 1244
TaiI ACGT 3 cut(s) 430, 780, 1068
TaqI TCGA 6 cut(s) 27, 72, 85, 307, 457, 1215
TasI AATT 5 cut(s) 170, 346, 626, 1392, 1808
TatI WGTACW 1 cut(s) 1696
TfiI GAWTC 6 cut(s) 452, 621, 846, 1114, 1307, 1595
Tru1I TTAA 8 cut(s) 528, 666, 1397, 1505, 1533, 1611, 1626, 1707
Tru9I TTAA 8 cut(s) 528, 666, 1397, 1505, 1533, 1611, 1626, 1707
TscAI CASTG 7 cut(s) 253, 304, 717, 843, 1189, 1497, 1572
TseFI GTSAC 4 cut(s) 42, 922, 1174, 1186
Tsp45I GTSAC 4 cut(s) 42, 922, 1174, 1186
TspDTI ATGAA 7 cut(s) 149, 212, 788, 1016, 1201, 1290, 1647
TspGWI ACGGA 1 cut(s) 1787
TspRI CASTG 7 cut(s) 253, 304, 717, 843, 1189, 1497, 1572
Tth111I GACNNNGTC 1 cut(s) 1184
VpaK11BI GGWCC 1 cut(s) 91
XapI RAATTY 3 cut(s) 626, 1392, 1808
XmnI GAANNNNTTC 2 cut(s) 620, 1281
XspI CTAG 3 cut(s) 1329, 1416, 1825
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.